Restriction Fragments for COX6/YHR051W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

COX6/YHR051W on chromosome VIII from coordinates 209705 to 210151.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.19722.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 15 + BmgT120I GGNCC 12 13 . . 11 15 + AsuI* GGNCC 11 14 . . 12 15 + CviJI RGCY 13 13 . . 12 15 + HaeIII GGCC 13 13 . . 20 24 + Hpy188I TCNGA 22 21 . . 28 32 - BsrI ACTGG 28 26 . . 32 37 + PsiI TTATAA 34 34 . . 54 57 + CviJI RGCY 55 55 . . 60 63 + SetI ASST 63 59 . . 60 66 + SexAI ACCWGGT 60 65 . . 61 65 + BssKI CCNGG 60 65 . . 61 65 + BseBI CCWGG 62 63 . . 61 65 + ScrFI CCNGG 62 63 . . 61 65 + EcoRII CCWGG 60 65 . . 67 77 + MwoI GCNNNNNNNGC 73 70 . . 73 76 + FatI CATG 72 76 . . 73 76 + NlaIII CATG 76 72 . . 73 76 + CviAII CATG 73 75 . . 75 78 + CviRI* TGCA 76 76 . . 80 83 + MaeI CTAG 80 82 . . 97 100 + TaiI ACGT 100 96 . . 97 100 + SetI ASST 100 96 . . 97 100 + MaeII ACGT 97 99 . . 121 125 + Hpy188I TCNGA 123 122 . . 124 128 + HgaI GACGC 133 138 . . 130 133 + FatI CATG 129 133 . . 130 133 + NlaIII CATG 133 129 . . 130 133 + CviAII CATG 130 132 . . 134 138 + TspDTI ATGAA 149 147 . . 136 140 + MboII GAAGA 148 147 . . 142 145 + SetI ASST 145 141 . . 148 151 - MnlI CCTC 141 140 . . 151 156 + EcoRI GAATTC 151 155 . . 151 156 + ApoI RAATTY 151 155 . . 152 155 + TspEI AATT 151 155 . . 158 161 + AciI CCGC 158 160 . . 155 159 - HphI GGTGA 147 146 . . 182 186 + TspDTI ATGAA 197 195 . . 186 189 + CviJI RGCY 187 187 . . 206 209 + CviRI* TGCA 207 207 . . 207 217 + MwoI GCNNNNNNNGC 213 210 . . 207 217 + BstAPI GCANNNNNTGC 213 210 . . 214 219 + SduI GDGCHC 218 214 . . 214 219 + HgiAI* GWGCWC 218 214 . . 224 228 + Tsp4CI* ACNGT 226 225 . . 254 259 - EcoP15I CAGCAG 226 228 . . 269 274 + HindIII AAGCTT 269 273 . . 270 273 + SetI ASST 273 269 . . 270 273 + CviJI RGCY 271 271 . . 270 273 + AluI AGCT 271 271 . . 271 281 + MwoI GCNNNNNNNGC 277 274 . . 276 279 - MnlI CCTC 269 268 . . 279 282 + CviJI RGCY 280 280 . . 280 284 + BlsI GCNGC 282 281 . . 280 284 + BisI GCNGC 281 282 . . 280 284 + TseI GCWGC 280 283 . . 280 284 - BbvI GCAGC 267 271 . . 287 291 + MboII GAAGA 299 298 . . 290 294 + PleI GAGTC 298 299 . . 290 294 + MlyI GAGTC 299 299 . . 290 294 + HinfI GANTC 290 293 . . 287 292 - Ksp632I* CTCTTC 282 285 . . 303 306 + SetI ASST 306 302 . . 308 311 + AciI CCGC 308 310 . . 312 315 + TspEI AATT 311 315 . . 327 330 + CviJI RGCY 328 328 . . 325 328 - MnlI CCTC 318 317 . . 341 344 + SetI ASST 344 340 . . 350 354 + TspDTI ATGAA 365 363 . . 352 356 + MboII GAAGA 364 363 . . 352 357 + BbvII* GAAGAC 359 363 . . 357 367 + BsiYI* CCNNNNNNNGG 363 360 . . 364 374 + FalI AAGNNNNNCTT 355 350 387 382 365 370 + StuI AGGCCT 367 367 . . 366 369 + CviJI RGCY 367 367 . . 366 369 + HaeIII GGCC 367 367 . . 364 374 - FalI AAGNNNNNCTT 387 382 355 350 375 379 + FokI GGATG 388 392 . . 375 379 + BseGI GGATG 381 379 . . 377 381 + TspDTI ATGAA 392 390 . . 380 383 + TspEI AATT 379 383 . . 383 394 + BdaI TGANNNNNNTCA 372 370 406 404 387 391 + FokI GGATG 400 404 . . 387 391 + BseGI GGATG 393 391 . . 384 388 - Hin4II* CCTTC 378 377 . . 392 396 + Hpy188I TCNGA 394 393 . . 383 394 - BdaI TGANNNNNNTCA 406 404 372 370 401 404 + TspEI AATT 400 404 . . 415 418 + MseI TTAA 415 417 . . 421 425 + MboII GAAGA 433 432 . . 425 428 + SetI ASST 428 424 . . 425 428 + CviJI RGCY 426 426 . . 425 428 + AluI AGCT 426 426 . . 421 426 - Ksp632I* CTCTTC 416 419 . . 421 427 - SapI GCTCTTC 416 419 . . 435 445 + FalI AAGNNNNNCTT 426 421 . . 436 439 + SetI ASST 439 435 . . 436 439 + CviJI RGCY 437 437 . . 436 439 + AluI AGCT 437 437 . . 439 443 - MboII GAAGA 431 430 . . 444 447 + MseI TTAA 444 446 . . #--------------------------------------- # # # AciI: # [CCGC] # 139 150 158 # # AluI: # [AGCT] # 10 11 155 271 # # ApoI: # [RAATTY] # 151 296 # # AsuI*: # [GGNCC] # 11 436 # # BbvI: # [GCAGC] # 180 267 # # BbvII*: # [GAAGAC] # 88 359 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 41 372 # # BisI: # [GCNGC] # 166 281 # # BlsI: # [GCNGC] # 165 282 # # BmgT120I: # [GGNCC] # 12 435 # # BseBI: # [CCWGG] # 62 385 # # BseGI: # [GGATG] # 12 54 381 # # BsiYI*: # [CCNNNNNNNGG] # 84 363 # # BsrI: # [ACTGG] # 28 419 # # BssKI: # [CCNGG] # 60 387 # # BstAPI: # [GCANNNNNTGC] # 213 234 # # CviAII: # [CATG] # 57 73 317 # # CviJI: # [RGCY] # 9 10 11 13 39 42 # 48 59 84 132 # # CviRI*: # [TGCA] # 76 131 240 # # EcoP15I: # [CAGCAG] # 221 226 # # EcoRI: # [GAATTC] # 151 296 # # EcoRII: # [CCWGG] # 60 387 # # FalI: # [AAGNNNNNCTT] # 0 0 21 32 39 355 # # FatI: # [CATG] # 57 72 318 # # FokI: # [GGATG] # 12 47 388 # # HaeIII: # [GGCC] # 13 80 354 # # HgaI: # [GACGC] # 133 314 # # HgiAI*: # [GWGCWC] # 218 229 # # Hin4II*: # [CCTTC] # 69 378 # # HindIII: # [AAGCTT] # 178 269 # # HinfI: # [GANTC] # 157 290 # # HphI: # [GGTGA] # 147 300 # # Hpy188I: # [TCNGA] # 22 53 101 271 # # Ksp632I*: # [CTCTTC] # 31 134 282 # # MaeI: # [CTAG] # 80 367 # # MaeII: # [ACGT] # 97 350 # # MboII: # [GAAGA] # 2 14 65 67 148 151 # # MlyI: # [GAGTC] # 148 299 # # MnlI: # [CCTC] # 49 128 129 141 # # MseI: # [TTAA] # 3 29 415 # # MwoI: # [GCNNNNNNNGC] # 64 73 140 170 # # NlaIII: # [CATG] # 57 76 314 # # PleI: # [GAGTC] # 149 298 # # PsiI: # [TTATAA] # 34 413 # # SapI: # [GCTCTTC] # 31 416 # # ScrFI: # [CCNGG] # 62 385 # # SduI: # [GDGCHC] # 218 229 # # SetI: # [ASST] # 8 11 33 37 38 45 # 63 84 128 # # SexAI: # [ACCWGGT] # 60 387 # # StuI: # [AGGCCT] # 80 367 # # TaiI: # [ACGT] # 100 347 # # TseI: # [GCWGC] # 167 280 # # Tsp4CI*: # [ACNGT] # 221 226 # # TspDTI: # [ATGAA] # 27 48 55 149 168 # # TspEI: # [AATT] # 21 47 68 151 160 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 447 # Reported_sequences: 1 # Reported_hitcount: 101 #---------------------------------------