Restriction Fragments for RPN1/YHR027C



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The currently selected gene/sequence is :

RPN1/YHR027C on chromosome VIII from coordinates 164711 to 161730.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20346.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + Hpy166II GTNNAC 6 6 . . 4 9 + AccI GTMKAC 5 7 . . 37 47 + Hin4I GAYNNNNNVTC 28 23 60 55 38 42 + TspDTI ATGAA 53 51 . . 37 47 - Hin4I GAYNNNNNVTC 60 55 28 23 57 62 + Hpy178III* TCNNGA 58 60 . . 81 85 - Hin4II* CCTTC 75 74 . . 97 100 - MnlI CCTC 90 89 . . 100 104 + MboII GAAGA 112 111 . . 103 108 + BseRI GAGGAG 118 116 . . 100 105 - Ksp632I* CTCTTC 95 98 . . 103 106 - MnlI CCTC 96 95 . . 108 112 + BlsI GCNGC 110 109 . . 108 112 + BisI GCNGC 109 110 . . 108 112 + BbvI GCAGC 120 124 . . 108 112 + TseI GCWGC 108 111 . . 110 113 + SetI ASST 113 109 . . 110 113 + CviJI RGCY 111 111 . . 110 113 + AluI AGCT 111 111 . . 115 119 + Hpy188I TCNGA 117 116 . . 106 116 - Hin4I GAYNNNNNVTC 129 124 97 92 117 128 + BcgI CGANNNNNNTGC 106 104 140 138 118 122 + MboII GAAGA 130 129 . . 121 125 + MboII GAAGA 133 132 . . 126 129 + CviRI* TGCA 127 127 . . 124 128 - SfaNI GCATC 114 118 . . 130 135 + HindIII AAGCTT 130 134 . . 131 134 + SetI ASST 134 130 . . 131 134 + CviJI RGCY 132 132 . . 131 134 + AluI AGCT 132 132 . . 134 137 + MseI TTAA 134 136 . . 149 152 + SetI ASST 152 148 . . 149 152 + CviJI RGCY 150 150 . . 149 152 + AluI AGCT 150 150 . . 164 167 + CviJI RGCY 165 165 . . 172 176 + MboII GAAGA 184 183 . . 172 177 + BbvII* GAAGAC 179 183 . . 175 185 + Hin4I GAYNNNNNVTC 166 161 198 193 178 182 + TfiI GAWTC 178 181 . . 178 182 + HinfI GANTC 178 181 . . 180 184 - TspDTI ATGAA 170 168 . . 175 185 - Hin4I GAYNNNNNVTC 198 193 166 161 191 195 + TspDTI ATGAA 206 204 . . 196 200 - HgaI GACGC 185 190 . . 204 209 + BsmI GAATGC 210 208 . . 211 214 + MseI TTAA 211 213 . . 217 221 + TfiI GAWTC 217 220 . . 217 221 + HinfI GANTC 217 220 . . 219 224 + ClaI ATCGAT 220 222 . . 220 223 + TaqI TCGA 220 222 . . 222 225 + DpnI GATC 223 223 . . 222 225 + BstKTI GATC 224 222 . . 222 225 + MboI GATC 221 225 . . 228 233 + ApoI RAATTY 228 232 . . 229 232 + TspEI AATT 228 232 . . 248 251 + AciI CCGC 248 250 . . 250 254 - BceAI ACGGC 235 237 . . 261 264 + SetI ASST 264 260 . . 268 273 + ApoI RAATTY 268 272 . . 269 272 + TspEI AATT 268 272 . . 273 277 + Hin4II* CCTTC 283 282 . . 279 284 + MmeI TCCRAC 304 302 . . 279 283 + Hpy188I TCNGA 281 280 . . 288 293 + Hpy178III* TCNNGA 289 291 . . 293 296 + SetI ASST 296 292 . . 321 326 + XhoII RGATCY 321 325 . . 322 325 + DpnI GATC 323 323 . . 322 325 + BstKTI GATC 324 322 . . 322 325 + MboI GATC 321 325 . . 322 326 - BinI* GGATC 316 317 . . 338 341 + MnlI CCTC 348 347 . . 348 352 + FokI GGATG 361 365 . . 348 352 + BseGI GGATG 354 352 . . 349 359 + Hin4I GAYNNNNNVTC 340 335 372 367 346 349 - AciI CCGC 346 348 . . 349 359 - Hin4I GAYNNNNNVTC 372 367 340 335 365 370 + TsoI TARCCA 381 379 . . 366 369 + CviJI RGCY 367 367 . . 369 372 + FatI CATG 368 372 . . 369 372 + NlaIII CATG 372 368 . . 369 372 + CviAII CATG 369 371 . . 379 383 + Hpy188I TCNGA 381 380 . . 389 394 + Hpy166II GTNNAC 391 391 . . 397 401 + TfiI GAWTC 397 400 . . 397 401 + HinfI GANTC 397 400 . . 399 403 - TspDTI ATGAA 389 387 . . 421 426 + MmeI TCCRAC 446 444 . . 421 425 + Hpy188I TCNGA 423 422 . . 423 427 + Hpy99I CGWCG 427 422 . . 425 428 + TaiI ACGT 428 424 . . 425 428 + SetI ASST 428 424 . . 425 428 + MaeII ACGT 425 427 . . 430 434 + Hpy188I TCNGA 432 431 . . 439 443 - Hin4II* CCTTC 433 432 . . 447 451 + BslFI GGGAC 461 465 . . 451 454 + FatI CATG 450 454 . . 451 454 + NlaIII CATG 454 450 . . 451 454 + CviAII CATG 451 453 . . 455 460 + TatI WGTACW 455 459 . . 456 459 + Csp6I GTAC 456 458 . . 456 459 + RsaI GTAC 457 457 . . 459 463 - FokI GGATG 445 449 . . 459 463 - BseGI GGATG 458 456 . . 461 465 - TspGWI ACGGA 451 449 . . 471 474 + SetI ASST 474 470 . . 471 474 + CviJI RGCY 472 472 . . 471 474 + AluI AGCT 472 472 . . 480 483 + DpnI GATC 481 481 . . 480 483 + BstKTI GATC 482 480 . . 480 483 + MboI GATC 479 483 . . 484 488 + HphI GGTGA 496 495 . . 498 503 + BclI TGATCA 498 502 . . 499 502 + DpnI GATC 500 500 . . 499 502 + BstKTI GATC 501 499 . . 499 502 + MboI GATC 498 502 . . 514 518 - SfaNI GCATC 504 508 . . 518 523 + Eco57MI CTGRAG 539 537 . . 518 523 + Eco57I CTGAAG 539 537 . . 520 524 + MboII GAAGA 532 531 . . 523 533 + Hin4I GAYNNNNNVTC 514 509 546 541 524 528 + TspDTI ATGAA 539 537 . . 523 533 - Hin4I GAYNNNNNVTC 546 541 514 509 535 539 + Hpy188I TCNGA 537 536 . . 541 546 + XhoII RGATCY 541 545 . . 541 545 + BinI* GGATC 549 550 . . 542 545 + DpnI GATC 543 543 . . 542 545 + BstKTI GATC 544 542 . . 542 545 + MboI GATC 541 545 . . 538 542 - BccI CCATC 532 533 . . 551 556 + BtgZI GCGATG 566 570 . . 552 563 + BcgI CGANNNNNNTGC 541 539 575 573 556 561 + XhoII RGATCY 556 560 . . 556 560 + BinI* GGATC 564 565 . . 557 560 + DpnI GATC 558 558 . . 557 560 + BstKTI GATC 559 557 . . 557 560 + MboI GATC 556 560 . . 553 557 - BccI CCATC 547 548 . . 562 566 + TauI GCSGC 565 562 . . 562 566 + BlsI GCNGC 564 563 . . 562 566 + BisI GCNGC 563 564 . . 564 567 + CviJI RGCY 565 565 . . 562 565 - AciI CCGC 562 564 . . 572 576 + BssKI CCNGG 571 576 . . 572 575 + HpaII CCGG 572 574 . . 572 576 + ScrFI CCNGG 573 574 . . 572 576 + CauII* CCSGG 573 574 . . 577 582 + AsuII TTCGAA 578 580 . . 578 581 + TaqI TCGA 578 580 . . 580 585 + ApoI RAATTY 580 584 . . 581 584 + TspEI AATT 580 584 . . 587 592 + StyI CCWWGG 587 591 . . 587 592 + SecI* CCNNGG 587 591 . . 592 596 + MboII GAAGA 604 603 . . 593 603 + FalI AAGNNNNNCTT 584 579 616 611 599 604 + Ksp632I* CTCTTC 605 608 . . 593 603 - FalI AAGNNNNNCTT 616 611 584 579 600 604 - MboII GAAGA 592 591 . . 616 624 + Tth111I GACNNNGTC 619 620 . . 622 626 - BslFI GGGAC 607 611 . . 640 649 + MslI CAYNNNNRTG 644 644 . . 640 649 + OliI CACNNNNGTG 644 644 . . 644 648 + Tsp4CI* ACNGT 646 645 . . 646 650 + HphI GGTGA 658 657 . . 649 653 + MboII GAAGA 661 660 . . 652 656 + MboII GAAGA 664 663 . . 652 657 + BbvII* GAAGAC 659 663 . . 655 659 + HgaI GACGC 664 669 . . 657 660 + FnuDII* CGCG 658 658 . . 658 661 - AciI CCGC 658 660 . . 678 681 + TspEI AATT 677 681 . . 682 686 + TfiI GAWTC 682 685 . . 682 686 + HinfI GANTC 682 685 . . 695 698 + SetI ASST 698 694 . . 695 698 + CviJI RGCY 696 696 . . 695 698 + AluI AGCT 696 696 . . 699 702 + SetI ASST 702 698 . . 700 703 + MnlI CCTC 710 709 . . 704 707 + TspEI AATT 703 707 . . 710 713 + TaqI TCGA 710 712 . . 717 726 + XmnI GAANNNNTTC 721 721 . . 721 724 + SetI ASST 724 720 . . 722 726 + Hin4II* CCTTC 732 731 . . 750 753 + CviRI* TGCA 751 751 . . 751 756 + NspI RCATGY 755 751 . . 752 755 + FatI CATG 751 755 . . 752 755 + NlaIII CATG 755 751 . . 752 755 + CviAII CATG 752 754 . . 771 774 + SetI ASST 774 770 . . 772 775 + MnlI CCTC 782 781 . . 774 779 + Hpy178III* TCNNGA 775 777 . . 776 781 + Eco57MI CTGRAG 797 795 . . 776 781 + Eco57I CTGAAG 797 795 . . 778 782 + MboII GAAGA 790 789 . . 778 783 + BbvII* GAAGAC 785 789 . . 782 785 + TaiI ACGT 785 781 . . 782 785 + SetI ASST 785 781 . . 782 785 + MaeII ACGT 782 784 . . 792 797 + AhaIII* TTTAAA 794 794 . . 793 796 + MseI TTAA 793 795 . . 800 803 + AciI CCGC 800 802 . . 821 825 + DdeI CTNAG 821 824 . . 821 825 + BspCNI CTCAG 834 832 . . 821 825 + BseMII CTCAG 835 833 . . 822 826 + Hpy188I TCNGA 824 823 . . 840 843 + CviRI* TGCA 841 841 . . 842 847 + MfeI CAATTG 842 846 . . 838 842 - SfaNI GCATC 828 832 . . 843 846 + TspEI AATT 842 846 . . 862 865 + MaeI CTAG 862 864 . . 868 872 + MboII GAAGA 880 879 . . 868 873 - Ksp632I* CTCTTC 863 866 . . 871 874 - MnlI CCTC 864 863 . . 878 883 + BclI TGATCA 878 882 . . 879 882 + DpnI GATC 880 880 . . 879 882 + BstKTI GATC 881 879 . . 879 882 + MboI GATC 878 882 . . 881 885 + Hpy188I TCNGA 883 882 . . 883 888 + XhoII RGATCY 883 887 . . 884 887 + DpnI GATC 885 885 . . 884 887 + BstKTI GATC 886 884 . . 884 887 + MboI GATC 883 887 . . 884 888 - BinI* GGATC 878 879 . . 886 890 - TspGWI ACGGA 876 874 . . 893 896 + TaqI TCGA 893 895 . . 895 899 - SfaNI GCATC 885 889 . . 902 905 + MaeI CTAG 902 904 . . 907 910 + DpnI GATC 908 908 . . 907 910 + BstKTI GATC 909 907 . . 907 910 + MboI GATC 906 910 . . 911 917 + TspRI CASTGNN 917 908 . . 907 911 - BinI* GGATC 901 902 . . 910 914 - BsrI ACTGG 910 908 . . 916 921 + EcoT22I ATGCAT 920 916 . . 917 920 + CviRI* TGCA 918 918 . . 915 919 - SfaNI GCATC 905 909 . . 926 929 + TspEI AATT 925 929 . . 942 945 + CviJI RGCY 943 943 . . 943 947 + TauI GCSGC 946 943 . . 943 947 + BlsI GCNGC 945 944 . . 943 947 + BisI GCNGC 944 945 . . 944 947 + AciI CCGC 944 946 . . 968 972 + TspDTI ATGAA 983 981 . . 969 980 + BdaI TGANNNNNNTCA 958 956 992 990 970 979 + XmnI GAANNNNTTC 974 974 . . 972 975 + SetI ASST 975 971 . . 970 974 - Hin4II* CCTTC 964 963 . . 978 983 + Hpy178III* TCNNGA 979 981 . . 969 980 - BdaI TGANNNNNNTCA 992 990 958 956 987 990 + TspEI AATT 986 990 . . 1001 1004 + TspEI AATT 1000 1004 . . 1006 1010 + Hpy188I TCNGA 1008 1007 . . 1034 1037 + TspEI AATT 1033 1037 . . 1038 1042 + TfiI GAWTC 1038 1041 . . 1038 1042 + HinfI GANTC 1038 1041 . . 1045 1049 + BsrI ACTGG 1050 1048 . . 1045 1050 + BfiI ACTGGG 1055 1054 . . 1048 1052 + BmgT120I GGNCC 1049 1050 . . 1048 1052 + AsuI* GGNCC 1048 1051 . . 1049 1052 + CviJI RGCY 1050 1050 . . 1049 1052 + HaeIII GGCC 1050 1050 . . 1057 1061 - BslFI GGGAC 1042 1046 . . 1063 1067 + MboII GAAGA 1075 1074 . . 1063 1068 + BbvII* GAAGAC 1070 1074 . . 1071 1076 + PsiI TTATAA 1073 1073 . . 1078 1081 + CviJI RGCY 1079 1079 . . 1107 1112 + SmlI CTYRAG 1107 1111 . . 1111 1116 + HaeII RGCGCY 1115 1111 . . 1112 1115 + Hin6I GCGC 1112 1114 . . 1112 1115 + GlaI GCGC 1113 1113 . . 1107 1112 - Bce83I* CTTGAG 1092 1090 . . 1112 1115 + HhaI GCGC 1114 1112 . . 1114 1119 + NaeI GCCGGC 1116 1116 . . 1114 1119 + MroNI GCCGGC 1114 1118 . . 1114 1119 + Cac8I GCNNGC 1116 1116 . . 1114 1119 + Cfr10I RCCGGY 1114 1118 . . 1115 1118 + HpaII CCGG 1115 1117 . . 1117 1120 + CviJI RGCY 1118 1118 . . 1117 1120 + HaeIII GGCC 1118 1118 . . 1120 1123 + MaeI CTAG 1120 1122 . . 1123 1127 + TfiI GAWTC 1123 1126 . . 1123 1127 + HinfI GANTC 1123 1126 . . 1141 1144 + MaeI CTAG 1141 1143 . . 1143 1146 + SetI ASST 1146 1142 . . 1143 1146 + CviJI RGCY 1144 1144 . . 1143 1146 + AluI AGCT 1144 1144 . . 1156 1161 + Hpy166II GTNNAC 1158 1158 . . 1156 1161 + HindII GTYRAC 1158 1158 . . 1168 1171 + MseI TTAA 1168 1170 . . 1173 1177 + DdeI CTNAG 1173 1176 . . 1176 1179 + SetI ASST 1179 1175 . . 1184 1188 + MaeIII GTNAC 1183 1188 . . 1193 1196 + TspEI AATT 1192 1196 . . 1194 1199 + VspI ATTAAT 1195 1197 . . 1195 1198 + MseI TTAA 1195 1197 . . 1197 1200 + TspEI AATT 1196 1200 . . 1213 1216 + TspEI AATT 1212 1216 . . 1233 1236 + SetI ASST 1236 1232 . . 1234 1238 + HphI GGTGA 1246 1245 . . 1235 1239 + Tsp45I GTSAC 1234 1239 . . 1235 1239 + MaeIII GTNAC 1234 1239 . . 1238 1242 + Tsp4CI* ACNGT 1240 1239 . . 1250 1255 + NspBII* CMGCKG 1252 1252 . . 1250 1255 + SacII CCGCGG 1253 1251 . . 1250 1255 + DsaI* CCRYGG 1250 1254 . . 1250 1255 + SecI* CCNNGG 1250 1254 . . 1250 1253 + AciI CCGC 1250 1252 . . 1251 1254 + FnuDII* CGCG 1252 1252 . . 1252 1255 - AciI CCGC 1252 1254 . . 1257 1260 + SetI ASST 1260 1256 . . 1257 1260 + CviJI RGCY 1258 1258 . . 1257 1260 + AluI AGCT 1258 1258 . . 1259 1262 + MaeI CTAG 1259 1261 . . 1267 1272 + XhoII RGATCY 1267 1271 . . 1267 1271 + BinI* GGATC 1275 1276 . . 1268 1271 + DpnI GATC 1269 1269 . . 1268 1271 + BstKTI GATC 1270 1268 . . 1268 1271 + MboI GATC 1267 1271 . . 1279 1285 + TspRI CASTGNN 1285 1276 . . 1278 1282 - BsrI ACTGG 1278 1276 . . 1284 1288 + TfiI GAWTC 1284 1287 . . 1284 1288 + HinfI GANTC 1284 1287 . . 1287 1292 + Hpy178III* TCNNGA 1288 1290 . . 1289 1292 + TaqI TCGA 1289 1291 . . 1291 1295 - BccI CCATC 1285 1286 . . 1298 1301 + CviRI* TGCA 1299 1299 . . 1305 1309 + BsrI ACTGG 1310 1308 . . 1309 1318 + BsaBI GATNNNNATC 1313 1313 . . 1322 1325 + TaiI ACGT 1325 1321 . . 1322 1325 + SetI ASST 1325 1321 . . 1322 1325 + MaeII ACGT 1322 1324 . . 1331 1334 + CviJI RGCY 1332 1332 . . 1340 1343 + MseI TTAA 1340 1342 . . 1344 1347 + SetI ASST 1347 1343 . . 1344 1347 + CviJI RGCY 1345 1345 . . 1344 1347 + AluI AGCT 1345 1345 . . 1350 1353 + CviRI* TGCA 1351 1351 . . 1359 1362 + SetI ASST 1362 1358 . . 1373 1376 + AciI CCGC 1373 1375 . . 1378 1383 + BetI* WCCGGW 1378 1382 . . 1379 1382 + HpaII CCGG 1379 1381 . . 1367 1379 - BarI GAAGNNNNNNTAC 1391 1386 1359 1354 1385 1388 + CviRI* TGCA 1386 1386 . . 1387 1396 + MslI CAYNNNNRTG 1391 1391 . . 1387 1390 + FatI CATG 1386 1390 . . 1387 1390 + NlaIII CATG 1390 1386 . . 1387 1390 + CviAII CATG 1387 1389 . . 1393 1397 + HphI GGTGA 1405 1404 . . 1390 1394 - BccI CCATC 1384 1385 . . 1407 1410 + SetI ASST 1410 1406 . . 1407 1410 + CviJI RGCY 1408 1408 . . 1407 1410 + AluI AGCT 1408 1408 . . 1413 1417 + MaeIII GTNAC 1412 1417 . . 1421 1424 + CviRI* TGCA 1422 1422 . . 1432 1436 + MaeIII GTNAC 1431 1436 . . 1452 1455 + TspEI AATT 1451 1455 . . 1460 1465 + EcoP15I CAGCAG 1490 1492 . . 1462 1466 + BlsI GCNGC 1464 1463 . . 1462 1466 + BisI GCNGC 1463 1464 . . 1462 1466 + BbvI GCAGC 1474 1478 . . 1462 1466 + TseI GCWGC 1462 1465 . . 1464 1467 + SetI ASST 1467 1463 . . 1464 1467 + CviJI RGCY 1465 1465 . . 1464 1467 + AluI AGCT 1465 1465 . . 1474 1477 + CviJI RGCY 1475 1475 . . 1474 1477 + HaeIII GGCC 1475 1475 . . 1476 1480 + BssKI CCNGG 1475 1480 . . 1476 1480 + BseBI CCWGG 1477 1478 . . 1476 1481 + SecI* CCNNGG 1476 1480 . . 1476 1480 + ScrFI CCNGG 1477 1478 . . 1476 1480 + EcoRII CCWGG 1475 1480 . . 1485 1488 + CviRI* TGCA 1486 1486 . . 1532 1537 + MfeI CAATTG 1532 1536 . . 1533 1536 + TspEI AATT 1532 1536 . . 1537 1541 + TauI GCSGC 1540 1537 . . 1537 1541 + BlsI GCNGC 1539 1538 . . 1537 1541 + BisI GCNGC 1538 1539 . . 1538 1541 + AciI CCGC 1538 1540 . . 1540 1544 + SfaNI GCATC 1549 1553 . . 1545 1551 + TspRI CASTGNN 1551 1542 . . 1541 1545 - FokI GGATG 1527 1531 . . 1541 1545 - BseGI GGATG 1540 1538 . . 1565 1570 + PstI CTGCAG 1569 1565 . . 1565 1570 + SfeI* CTRYAG 1565 1569 . . 1566 1569 + CviRI* TGCA 1567 1567 . . 1567 1577 + MwoI GCNNNNNNNGC 1573 1570 . . 1567 1571 + BlsI GCNGC 1569 1568 . . 1567 1571 + BisI GCNGC 1568 1569 . . 1567 1571 + BbvI GCAGC 1579 1583 . . 1567 1571 + TseI GCWGC 1567 1570 . . 1569 1572 + SetI ASST 1572 1568 . . 1569 1572 + CviJI RGCY 1570 1570 . . 1569 1572 + AluI AGCT 1570 1570 . . 1575 1578 + CviJI RGCY 1576 1576 . . 1576 1586 + MwoI GCNNNNNNNGC 1582 1579 . . 1580 1586 + Bpu10I CCTNAGC 1581 1584 . . 1581 1585 + DdeI CTNAG 1581 1584 . . 1584 1587 + SetI ASST 1587 1583 . . 1584 1587 + CviJI RGCY 1585 1585 . . 1584 1587 + AluI AGCT 1585 1585 . . 1590 1593 + CviJI RGCY 1591 1591 . . 1594 1597 + FatI CATG 1593 1597 . . 1594 1597 + NlaIII CATG 1597 1593 . . 1594 1597 + CviAII CATG 1594 1596 . . 1607 1610 + Csp6I GTAC 1607 1609 . . 1607 1610 + RsaI GTAC 1608 1608 . . 1618 1622 + HphI GGTGA 1630 1629 . . 1619 1623 + Tsp45I GTSAC 1618 1623 . . 1619 1623 + MaeIII GTNAC 1618 1623 . . 1638 1641 + FatI CATG 1637 1641 . . 1638 1641 + NlaIII CATG 1641 1637 . . 1638 1641 + CviAII CATG 1638 1640 . . 1658 1661 + Csp6I GTAC 1658 1660 . . 1658 1661 + RsaI GTAC 1659 1659 . . 1662 1665 + CviJI RGCY 1663 1663 . . 1664 1668 + BccI CCATC 1672 1673 . . 1667 1670 + TaqI TCGA 1667 1669 . . 1674 1678 + MboII GAAGA 1686 1685 . . 1674 1679 + BbvII* GAAGAC 1681 1685 . . 1677 1682 + TaqII GACCGA 1693 1691 . . 1682 1686 + BsrI ACTGG 1687 1685 . . 1682 1687 + BfiI ACTGGG 1692 1691 . . 1690 1693 + SetI ASST 1693 1689 . . 1710 1713 + SetI ASST 1713 1709 . . 1722 1725 + FatI CATG 1721 1725 . . 1722 1725 + NlaIII CATG 1725 1721 . . 1722 1725 + CviAII CATG 1722 1724 . . 1725 1729 + BmgT120I GGNCC 1726 1727 . . 1725 1729 + AsuI* GGNCC 1725 1728 . . 1726 1729 + CviJI RGCY 1727 1727 . . 1726 1729 + HaeIII GGCC 1727 1727 . . 1728 1733 + StyI CCWWGG 1728 1732 . . 1728 1733 + SecI* CCNNGG 1728 1732 . . 1731 1734 + SetI ASST 1734 1730 . . 1732 1736 + HphI GGTGA 1744 1743 . . 1733 1738 + Hpy166II GTNNAC 1735 1735 . . 1739 1742 + SetI ASST 1742 1738 . . 1743 1747 + FokI GGATG 1756 1760 . . 1743 1747 + BseGI GGATG 1749 1747 . . 1777 1781 - FokI GGATG 1763 1767 . . 1777 1781 - BseGI GGATG 1776 1774 . . 1791 1794 + SetI ASST 1794 1790 . . 1791 1794 + CviJI RGCY 1792 1792 . . 1791 1794 + AluI AGCT 1792 1792 . . 1798 1811 + AjuI GAANNNNNNNTTGG 1790 1785 1822 1817 1814 1817 + FatI CATG 1813 1817 . . 1814 1817 + NlaIII CATG 1817 1813 . . 1814 1817 + CviAII CATG 1814 1816 . . 1812 1816 - TspDTI ATGAA 1802 1800 . . 1825 1829 + BsrI ACTGG 1830 1828 . . 1829 1832 + Csp6I GTAC 1829 1831 . . 1829 1832 + RsaI GTAC 1830 1830 . . 1834 1838 + HphI GGTGA 1846 1845 . . 1846 1849 + MseI TTAA 1846 1848 . . 1848 1851 + TspEI AATT 1847 1851 . . 1851 1856 + Hpy178III* TCNNGA 1852 1854 . . 1860 1864 + MaeIII GTNAC 1859 1864 . . 1883 1886 + TaiI ACGT 1886 1882 . . 1883 1886 + SetI ASST 1886 1882 . . 1883 1886 + MaeII ACGT 1883 1885 . . 1890 1893 + SetI ASST 1893 1889 . . 1891 1895 + HphI GGTGA 1903 1902 . . 1894 1898 + MboII GAAGA 1906 1905 . . 1897 1901 + MboII GAAGA 1909 1908 . . 1902 1905 + CviRI* TGCA 1903 1903 . . 1900 1904 - SfaNI GCATC 1890 1894 . . 1907 1911 + TspDTI ATGAA 1922 1920 . . 1909 1913 + MboII GAAGA 1921 1920 . . 1912 1916 + MboII GAAGA 1924 1923 . . 1924 1928 - Hin4II* CCTTC 1918 1917 . . 1946 1950 + Tsp45I GTSAC 1945 1950 . . 1946 1950 + MaeIII GTNAC 1945 1950 . . 1957 1961 + HphI GGTGA 1969 1968 . . 1955 1960 - TaqII CACCCA 1945 1943 . . 1970 1974 + TspDTI ATGAA 1985 1983 . . 1974 1977 + SetI ASST 1977 1973 . . 1987 1990 - MnlI CCTC 1980 1979 . . 1992 1995 + SetI ASST 1995 1991 . . 1992 1995 + CviJI RGCY 1993 1993 . . 1992 1995 + AluI AGCT 1993 1993 . . 1994 1998 + DdeI CTNAG 1994 1997 . . 1998 2001 + DpnI GATC 1999 1999 . . 1998 2001 + BstKTI GATC 2000 1998 . . 1994 1998 - BspCNI CTCAG 1986 1984 . . 1994 1998 - BseMII CTCAG 1985 1983 . . 1998 2001 + MboI GATC 1997 2001 . . 2000 2003 + TaqI TCGA 2000 2002 . . 2005 2010 + Hpy166II GTNNAC 2007 2007 . . 2018 2021 + SetI ASST 2021 2017 . . 2017 2020 - MnlI CCTC 2010 2009 . . 2023 2027 - SfaNI GCATC 2013 2017 . . 2029 2033 - Hin4II* CCTTC 2023 2022 . . 2035 2038 - MnlI CCTC 2028 2027 . . 2032 2042 - BsaXI ACNNNNNCTCC 2054 2051 2024 2021 2048 2051 + MseI TTAA 2048 2050 . . 2067 2071 + MboII GAAGA 2079 2078 . . 2070 2074 + MboII GAAGA 2082 2081 . . 2077 2081 + HphI GGTGA 2089 2088 . . 2091 2094 + SetI ASST 2094 2090 . . 2092 2096 + HphI GGTGA 2104 2103 . . 2089 2093 - Hin4II* CCTTC 2083 2082 . . 2095 2099 + MboII GAAGA 2107 2106 . . 2095 2100 - Ksp632I* CTCTTC 2090 2093 . . 2107 2111 + Hpy188I TCNGA 2109 2108 . . 2098 2108 - Hin4I GAYNNNNNVTC 2121 2116 2089 2084 2110 2114 + MboII GAAGA 2122 2121 . . 2118 2122 - Hin4II* CCTTC 2112 2111 . . 2123 2132 + XmnI GAANNNNTTC 2127 2127 . . 2131 2135 + Hpy188I TCNGA 2133 2132 . . 2128 2132 - MboII GAAGA 2120 2119 . . 2133 2144 + BcgI CGANNNNNNTGC 2122 2120 2156 2154 2139 2143 + FokI GGATG 2152 2156 . . 2139 2143 + BseGI GGATG 2145 2143 . . 2140 2144 - SfaNI GCATC 2130 2134 . . 2149 2153 + TspGWI ACGGA 2164 2162 . . 2170 2175 + BseRI GAGGAG 2185 2183 . . 2170 2173 - MnlI CCTC 2163 2162 . . 2173 2176 - MnlI CCTC 2166 2165 . . 2176 2180 + MboII GAAGA 2188 2187 . . 2188 2193 + Cac8I GCNNGC 2190 2190 . . 2198 2201 + TaqI TCGA 2198 2200 . . 2199 2210 + BcgI CGANNNNNNTGC 2188 2186 2222 2220 2201 2205 + TspDTI ATGAA 2216 2214 . . 2214 2217 + CviRI* TGCA 2215 2215 . . 2230 2240 + MwoI GCNNNNNNNGC 2236 2233 . . 2241 2246 + StyI CCWWGG 2241 2245 . . 2241 2246 + SecI* CCNNGG 2241 2245 . . 2245 2249 + HphI GGTGA 2257 2256 . . 2248 2252 + MboII GAAGA 2260 2259 . . 2274 2277 + TaiI ACGT 2277 2273 . . 2274 2277 + SetI ASST 2277 2273 . . 2274 2277 + MaeII ACGT 2274 2276 . . 2290 2293 + MseI TTAA 2290 2292 . . 2293 2298 + EcoT22I ATGCAT 2297 2293 . . 2294 2297 + CviRI* TGCA 2295 2295 . . 2306 2310 + TspDTI ATGAA 2321 2319 . . 2316 2326 + BsiYI* CCNNNNNNNGG 2322 2319 . . 2317 2321 + Hpy99I CGWCG 2321 2316 . . 2313 2317 - FokI GGATG 2299 2303 . . 2313 2317 - BseGI GGATG 2312 2310 . . 2315 2319 - TspGWI ACGGA 2305 2303 . . 2325 2330 + NlaIV GGNNCC 2327 2327 . . 2335 2340 + BtgZI GCGATG 2350 2354 . . 2337 2341 - BccI CCATC 2331 2332 . . 2350 2354 - TspGWI ACGGA 2340 2338 . . 2356 2360 + Hpy188I TCNGA 2358 2357 . . 2359 2362 + DpnI GATC 2360 2360 . . 2359 2362 + BstKTI GATC 2361 2359 . . 2359 2362 + MboI GATC 2358 2362 . . 2359 2363 - BinI* GGATC 2353 2354 . . 2368 2372 + TspDTI ATGAA 2383 2381 . . 2370 2379 + XmnI GAANNNNTTC 2374 2374 . . 2372 2375 + SetI ASST 2375 2371 . . 2370 2374 - Hin4II* CCTTC 2364 2363 . . 2378 2381 + TaqI TCGA 2378 2380 . . 2383 2386 + SetI ASST 2386 2382 . . 2386 2389 + MseI TTAA 2386 2388 . . 2401 2404 + FatI CATG 2400 2404 . . 2401 2404 + NlaIII CATG 2404 2400 . . 2401 2404 + CviAII CATG 2401 2403 . . 2404 2408 + HgaI GACGC 2413 2418 . . 2404 2409 + AcyI GRCGYC 2405 2407 . . 2425 2429 + TspDTI ATGAA 2440 2438 . . 2427 2432 + EcoRI GAATTC 2427 2431 . . 2427 2432 + ApoI RAATTY 2427 2431 . . 2428 2431 + TspEI AATT 2427 2431 . . 2433 2436 + TspEI AATT 2432 2436 . . 2462 2465 + Csp6I GTAC 2462 2464 . . 2462 2465 + RsaI GTAC 2463 2463 . . 2472 2475 + CviRI* TGCA 2473 2473 . . 2473 2483 + MwoI GCNNNNNNNGC 2479 2476 . . 2476 2479 + SetI ASST 2479 2475 . . 2481 2484 + SetI ASST 2484 2480 . . 2481 2484 + CviJI RGCY 2482 2482 . . 2481 2484 + AluI AGCT 2482 2482 . . 2491 2494 + MseI TTAA 2491 2493 . . 2496 2500 + Tsp4CI* ACNGT 2498 2497 . . 2516 2521 + BsaAI YACGTR 2518 2518 . . 2516 2521 + PmaCI CACGTG 2518 2518 . . 2517 2520 + TaiI ACGT 2520 2516 . . 2517 2520 + SetI ASST 2520 2516 . . 2517 2520 + MaeII ACGT 2517 2519 . . 2519 2524 + Hpy166II GTNNAC 2521 2521 . . 2527 2531 + HgaI GACGC 2536 2541 . . 2540 2544 - HphI GGTGA 2532 2531 . . 2564 2567 + CviRI* TGCA 2565 2565 . . 2565 2569 + SfaNI GCATC 2574 2578 . . 2577 2580 + SetI ASST 2580 2576 . . 2579 2582 + Csp6I GTAC 2579 2581 . . 2579 2582 + RsaI GTAC 2580 2580 . . 2592 2596 + FokI GGATG 2605 2609 . . 2592 2596 + BseGI GGATG 2598 2596 . . 2607 2610 + CviRI* TGCA 2608 2608 . . 2605 2609 - SfaNI GCATC 2595 2599 . . 2610 2615 + BspLU11I* ACATGT 2610 2614 . . 2610 2615 + NspI RCATGY 2614 2610 . . 2610 2615 + AflIII ACRYGT 2610 2614 . . 2611 2614 + FatI CATG 2610 2614 . . 2611 2614 + NlaIII CATG 2614 2610 . . 2611 2614 + CviAII CATG 2611 2613 . . 2624 2627 + SetI ASST 2627 2623 . . 2625 2629 + HphI GGTGA 2637 2636 . . 2626 2630 + Tsp45I GTSAC 2625 2630 . . 2626 2630 + MaeIII GTNAC 2625 2630 . . 2635 2639 + SfaNI GCATC 2644 2648 . . 2640 2645 + SspI AATATT 2642 2642 . . 2644 2647 + MseI TTAA 2644 2646 . . 2651 2654 + AciI CCGC 2651 2653 . . 2679 2682 + FatI CATG 2678 2682 . . 2679 2682 + NlaIII CATG 2682 2678 . . 2679 2682 + CviAII CATG 2679 2681 . . 2677 2681 - TspDTI ATGAA 2667 2665 . . 2688 2692 + SfaNI GCATC 2697 2701 . . 2695 2700 + MfeI CAATTG 2695 2699 . . 2696 2699 + TspEI AATT 2695 2699 . . 2705 2710 + BspLU11I* ACATGT 2705 2709 . . 2705 2710 + NspI RCATGY 2709 2705 . . 2705 2710 + AflIII ACRYGT 2705 2709 . . 2706 2709 + FatI CATG 2705 2709 . . 2706 2709 + NlaIII CATG 2709 2705 . . 2706 2709 + CviAII CATG 2706 2708 . . 2712 2717 + BsmI GAATGC 2718 2716 . . 2716 2721 + Cfr10I RCCGGY 2716 2720 . . 2717 2720 + HpaII CCGG 2717 2719 . . 2717 2727 + BsiYI* CCNNNNNNNGG 2723 2720 . . 2726 2729 + CviJI RGCY 2727 2727 . . 2726 2729 + HaeIII GGCC 2727 2727 . . 2740 2743 + MaeI CTAG 2740 2742 . . 2746 2749 + MseI TTAA 2746 2748 . . 2755 2758 - MnlI CCTC 2748 2747 . . 2762 2765 + CviJI RGCY 2763 2763 . . 2771 2774 + SetI ASST 2774 2770 . . 2774 2777 + MseI TTAA 2774 2776 . . 2781 2784 + Hin6I GCGC 2781 2783 . . 2781 2784 + GlaI GCGC 2782 2782 . . 2781 2784 + HhaI GCGC 2783 2781 . . 2782 2785 + FnuDII* CGCG 2783 2783 . . 2794 2799 + BtsI GCAGTG 2801 2799 . . 2795 2801 + TspRI CASTGNN 2801 2792 . . 2803 2807 + Tsp4CI* ACNGT 2805 2804 . . 2808 2813 + CfrI YGGCCR 2808 2812 . . 2809 2812 + CviJI RGCY 2810 2810 . . 2809 2812 + HaeIII GGCC 2810 2810 . . 2810 2820 + MwoI GCNNNNNNNGC 2816 2813 . . 2810 2820 + BglI GCCNNNNNGGC 2816 2813 . . 2815 2820 + Cac8I GCNNGC 2817 2817 . . 2836 2840 + BsrI ACTGG 2841 2839 . . 2839 2842 + CviJI RGCY 2840 2840 . . 2875 2878 + FatI CATG 2874 2878 . . 2875 2878 + NlaIII CATG 2878 2874 . . 2875 2878 + CviAII CATG 2875 2877 . . 2893 2896 + MaeI CTAG 2893 2895 . . 2903 2907 + TspDTI ATGAA 2918 2916 . . 2915 2919 + MaeIII GTNAC 2914 2919 . . 2923 2926 + CviJI RGCY 2924 2924 . . 2925 2935 + BsiYI* CCNNNNNNNGG 2931 2928 . . 2932 2935 - MnlI CCTC 2925 2924 . . 2943 2946 + TspEI AATT 2942 2946 . . 2946 2951 + AhaIII* TTTAAA 2948 2948 . . 2947 2950 + MseI TTAA 2947 2949 . . 2952 2956 + MboII GAAGA 2964 2963 . . 2967 2972 + Hpy178III* TCNNGA 2968 2970 . . 2971 2976 - Ksp632I* CTCTTC 2966 2969 . . 2974 2977 - MnlI CCTC 2967 2966 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 5 2977 # # AciI: # [CCGC] # 2 96 98 121 142 144 # 165 216 248 306 331 1113 # # AcyI: # [GRCGYC] # 577 2405 # # AflIII: # [ACRYGT] # 95 277 2610 # # AhaIII*: # [TTTAAA] # 34 794 2154 # # AjuI: # [GAANNNNNNNTTGG] # 32 1160 1790 # # AluI: # [AGCT] # 15 18 21 57 63 87 # 105 111 114 201 207 224 # 322 448 489 500 # # ApoI: # [RAATTY] # 40 228 312 555 1847 # # AsuI*: # [GGNCC] # 677 1048 1257 # # AsuII: # [TTCGAA] # 578 2404 # # BarI: # [GAAGNNNNNNTAC] # 32 1359 1591 # # BbvI: # [GCAGC] # 105 120 1354 1403 # # BbvII*: # [GAAGAC] # 126 179 285 480 611 1301 # # BccI: # [CCATC] # 15 99 288 532 651 659 # 738 # # Bce83I*: # [CTTGAG] # 1092 1890 # # BceAI: # [ACGGC] # 235 2747 # # BcgI: # [CGANNNNNNTGC] # 32 34 34 34 34 106 # 401 760 1547 # # BclI: # [TGATCA] # 380 498 2104 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 958 1990 # # BetI*: # [WCCGGW] # 1378 1604 # # BfiI: # [ACTGGG] # 637 1055 1290 # # BglI: # [GCCNNNNNGGC] # 166 2816 # # BinI*: # [GGATC] # 15 23 233 314 316 374 # 629 1078 # # BisI: # [GCNGC] # 30 75 109 381 454 519 # 1414 # # BlsI: # [GCNGC] # 30 75 110 381 454 519 # 1413 # # BmgT120I: # [GGNCC] # 677 1049 1256 # # Bpu10I: # [CCTNAGC] # 1401 1581 # # BsaAI: # [YACGTR] # 464 2518 # # BsaBI: # [GATNNNNATC] # 1313 1669 # # BsaXI: # [ACNNNNNCTCC] # 30 928 2024 # # BseBI: # [CCWGG] # 1477 1505 # # BseGI: # [GGATG] # 27 104 167 209 286 354 # 369 384 1082 # # BseMII: # [CTCAG] # 835 997 1150 # # BseRI: # [GAGGAG] # 118 797 2067 # # BsiYI*: # [CCNNNNNNNGG] # 51 208 401 2322 # # BslFI: # [GGGAC] # 146 435 461 1940 # # BsmI: # [GAATGC] # 210 264 2508 # # BspCNI: # [CTCAG] # 834 996 1152 # # BspLU11I*: # [ACATGT] # 95 277 2610 # # BsrI: # [ACTGG] # 32 140 141 143 228 377 # 910 1011 # # BssKI: # [CCNGG] # 571 904 1507 # # BstKTI: # [GATC] # 5 15 19 23 43 100 # 158 224 322 361 361 621 # 730 # # BtgZI: # [GCGATG] # 566 632 1784 # # BtsI: # [GCAGTG] # 181 2801 # # Cac8I: # [GCNNGC] # 165 627 1074 1116 # # CauII*: # [CCSGG] # 573 2409 # # Cfr10I: # [RCCGGY] # 266 1114 1602 # # CfrI: # [YGGCCR] # 174 2808 # # ClaI: # [ATCGAT] # 220 2762 # # Csp6I: # [GTAC] # 51 117 171 403 456 633 # 1151 # # CviAII: # [CATG] # 27 44 68 82 84 92 # 107 169 207 210 301 369 # 587 635 # # CviJI: # [RGCY] # 6 6 9 10 13 15 # 18 21 26 29 30 36 # 39 47 57 58 63 64 # 65 72 74 84 93 95 # 105 107 111 114 131 201 # 202 245 247 489 # # CviRI*: # [TGCA] # 35 36 43 52 64 77 # 80 81 90 92 127 178 # 312 336 374 381 624 # # DdeI: # [CTNAG] # 352 408 413 821 988 # # DpnI: # [GATC] # 5 15 19 23 43 100 # 158 223 322 361 361 622 # 730 # # DsaI*: # [CCRYGG] # 1250 1732 # # Eco57I: # [CTGAAG] # 258 539 2185 # # Eco57MI: # [CTGRAG] # 258 539 2185 # # EcoP15I: # [CAGCAG] # 1490 1492 # # EcoRI: # [GAATTC] # 555 2427 # # EcoRII: # [CCWGG] # 1475 1507 # # EcoT22I: # [ATGCAT] # 685 920 1377 # # FalI: # [AAGNNNNNCTT] # 0 0 32 584 2366 # # FatI: # [CATG] # 27 44 68 82 84 92 # 108 169 207 210 301 368 # 587 635 # # FnuDII*: # [CGCG] # 199 594 658 1531 # # FokI: # [GGATG] # 7 84 147 229 306 361 # 377 389 1082 # # GlaI: # [GCGC] # 200 1113 1669 # # HaeII: # [RGCGCY] # 1115 1867 # # HaeIII: # [GGCC] # 68 83 172 252 357 1000 # 1050 # # HgaI: # [GACGC] # 123 185 446 479 1749 # # HhaI: # [GCGC] # 199 1114 1669 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 28 32 32 32 # 32 32 32 37 37 142 # 142 861 1543 # # Hin4II*: # [CCTTC] # 29 60 75 105 150 208 # 232 252 299 618 954 # # Hin6I: # [GCGC] # 201 1112 1669 # # HindII: # [GTYRAC] # 1158 1824 # # HindIII: # [AAGCTT] # 130 2852 # # HinfI: # [GANTC] # 39 85 161 178 180 285 # 356 1698 # # HpaII: # [CCGG] # 264 265 543 572 1338 # # HphI: # [GGTGA] # 15 57 66 102 105 114 # 120 153 159 162 225 275 # 345 496 588 # # Hpy166II: # [GTNNAC] # 6 272 385 461 514 577 # 767 # # Hpy178III*: # [TCNNGA] # 14 58 204 231 309 486 # 564 1116 # # Hpy188I: # [TCNGA] # 9 24 42 59 100 105 # 117 125 164 225 287 624 # 1101 # # Hpy99I: # [CGWCG] # 427 661 1894 # # Ksp632I*: # [CTCTTC] # 16 95 258 510 876 1227 # # MaeI: # [CTAG] # 21 40 89 118 153 218 # 862 1481 # # MaeII: # [ACGT] # 243 357 391 425 465 540 # 561 # # MaeIII: # [GTNAC] # 19 51 68 86 178 187 # 241 289 680 1183 # # MboI: # [GATC] # 5 15 19 23 43 100 # 158 221 322 361 361 624 # 730 # # MboII: # [GAAGA] # 2 3 3 3 3 3 # 12 12 13 18 18 25 # 51 57 60 66 72 90 # 112 126 155 195 220 348 # 611 704 # # MfeI: # [CAATTG] # 287 690 842 1163 # # MmeI: # [TCCRAC] # 142 304 2536 # # MnlI: # [CCTC] # 3 6 15 18 30 42 # 72 82 90 135 177 252 # 362 582 1116 # # MroNI: # [GCCGGC] # 1114 1868 # # MseI: # [TTAA] # 27 28 35 77 96 102 # 105 134 145 153 173 202 # 242 375 506 582 # # MslI: # [CAYNNNNRTG] # 644 747 1591 # # MwoI: # [GCNNNNNNNGC] # 9 166 243 337 654 1573 # # NaeI: # [GCCGGC] # 1116 1866 # # NlaIII: # [CATG] # 27 44 68 82 84 92 # 104 169 207 210 301 372 # 587 635 # # NlaIV: # [GGNNCC] # 655 2327 # # NspBII*: # [CMGCKG] # 1252 1730 # # NspI: # [RCATGY] # 95 273 755 1859 # # OliI: # [CACNNNNGTG] # 644 2338 # # PmaCI: # [CACGTG] # 464 2518 # # PsiI: # [TTATAA] # 1073 1909 # # PstI: # [CTGCAG] # 1413 1569 # # RsaI: # [GTAC] # 51 117 171 402 457 633 # 1151 # # SacII: # [CCGCGG] # 1253 1729 # # ScrFI: # [CCNGG] # 573 904 1505 # # SecI*: # [CCNNGG] # 226 252 513 587 663 741 # # SetI: # [ASST] # 4 5 7 8 11 11 # 15 15 18 18 20 21 # 21 22 22 24 26 32 # 33 36 46 47 48 50 # 52 57 57 60 65 73 # 84 92 93 98 105 106 # 112 113 132 147 171 183 # 190 208 224 # # SfaNI: # [GCATC] # 20 21 49 53 57 114 # 117 123 285 324 341 390 # 444 644 # # SfeI*: # [CTRYAG] # 1417 1565 # # SmlI: # [CTYRAG] # 1107 1875 # # SspI: # [AATATT] # 340 2642 # # StyI: # [CCWWGG] # 513 587 741 1141 # # TaiI: # [ACGT] # 243 357 391 428 462 540 # 561 # # TaqI: # [TCGA] # 132 180 183 198 220 333 # 358 378 396 604 # # TaqII: # [GACCGA] # 252 1037 1693 # # TatI: # [WGTACW] # 455 2527 # # TauI: # [GCSGC] # 381 565 594 1442 # # TfiI: # [GAWTC] # 39 85 161 178 180 285 # 356 1698 # # TseI: # [GCWGC] # 105 108 1354 1415 # # TsoI: # [TARCCA] # 381 2601 # # Tsp45I: # [GTSAC] # 327 357 384 680 1234 # # Tsp4CI*: # [ACNGT] # 177 307 594 646 1258 # # TspDTI: # [ATGAA] # 36 53 57 62 63 64 # 105 117 120 150 183 227 # 231 251 444 819 # # TspEI: # [AATT] # 4 5 14 16 26 33 # 40 40 61 81 83 97 # 139 159 228 239 247 263 # 312 315 580 # # TspGWI: # [ACGGA] # 35 141 425 451 642 1288 # # TspRI: # [CASTGNN] # 181 266 368 917 1250 # # Tth111I: # [GACNNNGTC] # 619 2363 # # VspI: # [ATTAAT] # 1195 1787 # # XhoII: # [RGATCY] # 15 220 321 327 384 1715 # # XmnI: # [GAANNNNTTC] # 247 253 608 721 1153 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2982 # Reported_sequences: 1 # Reported_hitcount: 663 #---------------------------------------