Restriction Fragments for YHLCsigma1



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The currently selected gene/sequence is :

YHLCsigma1 on chromosome VIII from coordinates 85538 to 85388.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20062.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 5 9 + TfiI GAWTC 5 8 . . 5 9 + HinfI GANTC 5 8 . . 37 41 + MaeIII GTNAC 36 41 . . 44 48 - TspGWI ACGGA 34 32 . . 50 53 + TspEI AATT 49 53 . . 51 56 + VspI ATTAAT 52 54 . . 52 55 + MseI TTAA 52 54 . . 56 60 + Hpy188I TCNGA 58 57 . . 64 68 + Tsp4CI* ACNGT 66 65 . . 76 80 + BsrI ACTGG 81 79 . . 80 84 + MaeIII GTNAC 79 84 . . 78 83 - TsoI TARCCA 68 66 . . 89 93 + DdeI CTNAG 89 92 . . 94 98 + Tsp4CI* ACNGT 96 95 . . 113 118 + Hpy178III* TCNNGA 114 116 . . 122 127 + BetI* WCCGGW 122 126 . . 123 126 + HpaII CCGG 123 125 . . 131 138 + PspXI VCTCGAGB 132 136 . . 132 137 + SmlI CTYRAG 132 136 . . 132 137 + XhoI CTCGAG 132 136 . . 132 137 + BmeT110I CYCGRG 133 135 . . 132 137 + AvaI CYCGRG 132 136 . . 133 136 + TaqI TCGA 133 135 . . 135 140 + SduI GDGCHC 139 135 . . 135 140 + HgiJII* GRGCYC 139 135 . . 136 139 + CviJI RGCY 137 137 . . #--------------------------------------- # # # AvaI: # [CYCGRG] # 19 132 # # BetI*: # [WCCGGW] # 29 122 # # BmeT110I: # [CYCGRG] # 18 133 # # BsrI: # [ACTGG] # 70 81 # # CviJI: # [RGCY] # 14 137 # # DdeI: # [CTNAG] # 62 89 # # HgiJII*: # [GRGCYC] # 12 139 # # HinfI: # [GANTC] # 5 146 # # HpaII: # [CCGG] # 28 123 # # Hpy178III*: # [TCNNGA] # 37 114 # # Hpy188I: # [TCNGA] # 58 93 # # MaeIII: # [GTNAC] # 36 43 72 # # MseI: # [TTAA] # 52 99 # # PspXI: # [VCTCGAGB] # 19 132 # # SduI: # [GDGCHC] # 12 139 # # SmlI: # [CTYRAG] # 19 132 # # TaqI: # [TCGA] # 18 133 # # TfiI: # [GAWTC] # 5 146 # # TsoI: # [TARCCA] # 68 83 # # Tsp4CI*: # [ACNGT] # 30 55 66 # # TspEI: # [AATT] # 49 102 # # TspGWI: # [ACGGA] # 34 117 # # VspI: # [ATTAAT] # 52 99 # # XhoI: # [CTCGAG] # 19 132 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 151 # Reported_sequences: 1 # Reported_hitcount: 26 #---------------------------------------