Restriction Fragments for YHL046W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YHL046W-A on chromosome VIII from coordinates 9981 to 10307.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.13045.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 3 8 + BsmI GAATGC 9 7 . . 6 9 + CviRI* TGCA 7 7 . . 24 27 + SetI ASST 27 23 . . 24 27 + CviJI RGCY 25 25 . . 24 27 + AluI AGCT 25 25 . . 29 32 + MseI TTAA 29 31 . . 31 34 + TspEI AATT 30 34 . . 53 58 + TsoI TARCCA 69 67 . . 54 57 + CviJI RGCY 55 55 . . 61 64 + SetI ASST 64 60 . . 61 64 + CviJI RGCY 62 62 . . 61 64 + AluI AGCT 62 62 . . 63 67 + DdeI CTNAG 63 66 . . 63 67 + BspCNI CTCAG 76 74 . . 63 67 + BseMII CTCAG 77 75 . . 64 68 + Hpy188I TCNGA 66 65 . . 98 101 + MseI TTAA 98 100 . . 100 103 + TspEI AATT 99 103 . . 109 113 - TspDTI ATGAA 99 97 . . 114 118 - TspDTI ATGAA 104 102 . . 126 130 + Hpy99I CGWCG 130 125 . . 127 137 + Hin4I GAYNNNNNVTC 118 113 150 145 127 137 + Eam1105I GACNNNNNGTC 132 131 . . 130 134 + TfiI GAWTC 130 133 . . 130 134 + HinfI GANTC 130 133 . . 127 137 - Hin4I GAYNNNNNVTC 150 145 118 113 140 144 + BccI CCATC 148 149 . . 137 141 - FokI GGATG 123 127 . . 137 141 - BseGI GGATG 136 134 . . 147 151 - TspDTI ATGAA 137 135 . . 155 159 + BccI CCATC 163 164 . . 162 166 - TspDTI ATGAA 152 150 . . 170 173 + TspEI AATT 169 173 . . 200 203 + CviJI RGCY 201 201 . . 202 205 + MaeI CTAG 202 204 . . 199 204 - TsoI TARCCA 189 187 . . 214 218 - MboII GAAGA 206 205 . . 213 218 - BbvII* GAAGAC 206 210 . . 220 224 + BslFI GGGAC 234 238 . . 226 229 + TaqI TCGA 226 228 . . 228 231 + DpnI GATC 229 229 . . 228 231 + BstKTI GATC 230 228 . . 228 231 + MboI GATC 227 231 . . 243 246 + CviJI RGCY 244 244 . . 243 246 + HaeIII GGCC 244 244 . . 243 247 + BmgT120I GGNCC 244 245 . . 243 247 + AsuI* GGNCC 243 246 . . 253 257 + MaeIII GTNAC 252 257 . . 259 263 + Tsp4CI* ACNGT 261 260 . . 269 274 + SacI GAGCTC 273 269 . . 269 274 + Ecl136II GAGCTC 271 271 . . 269 274 + SduI GDGCHC 273 269 . . 269 274 + HgiJII* GRGCYC 273 269 . . 269 274 + HgiAI* GWGCWC 273 269 . . 270 273 + SetI ASST 273 269 . . 270 273 + CviJI RGCY 271 271 . . 270 273 + AluI AGCT 271 271 . . 272 277 + SmlI CTYRAG 272 276 . . 272 277 - Bce83I* CTTGAG 257 255 . . 268 278 - BsaXI ACNNNNNCTCC 290 287 260 257 268 279 - TstI CACNNNNNNTCC 292 287 260 255 299 303 - MboII GAAGA 291 290 . . 306 310 + BssKI CCNGG 305 310 . . 306 310 + BseBI CCWGG 307 308 . . 306 310 + ScrFI CCNGG 307 308 . . 306 310 + EcoRII CCWGG 305 310 . . 309 314 + XhoII RGATCY 309 313 . . 309 313 + BinI* GGATC 317 318 . . 310 320 + Hin4I GAYNNNNNVTC 301 296 . . 310 313 + DpnI GATC 311 311 . . 310 313 + BstKTI GATC 312 310 . . 310 313 + MboI GATC 309 313 . . 315 318 + FatI CATG 314 318 . . 315 318 + NlaIII CATG 318 314 . . 315 318 + CviAII CATG 315 317 . . #--------------------------------------- # # # AluI: # [AGCT] # 25 37 56 209 # # AsuI*: # [GGNCC] # 84 243 # # BbvII*: # [GAAGAC] # 121 206 # # BccI: # [CCATC] # 15 148 164 # # Bce83I*: # [CTTGAG] # 70 257 # # BinI*: # [GGATC] # 10 317 # # BmgT120I: # [GGNCC] # 83 244 # # BsaXI: # [ACNNNNNCTCC] # 30 37 260 # # BseBI: # [CCWGG] # 20 307 # # BseGI: # [GGATG] # 136 191 # # BseMII: # [CTCAG] # 77 250 # # BslFI: # [GGGAC] # 93 234 # # BsmI: # [GAATGC] # 9 318 # # BspCNI: # [CTCAG] # 76 251 # # BssKI: # [CCNGG] # 22 305 # # BstKTI: # [GATC] # 15 82 230 # # CviAII: # [CATG] # 12 315 # # CviJI: # [RGCY] # 7 25 27 30 43 56 # 139 # # CviRI*: # [TGCA] # 7 320 # # DdeI: # [CTNAG] # 63 264 # # DpnI: # [GATC] # 16 82 229 # # Eam1105I: # [GACNNNNNGTC] # 132 195 # # Ecl136II: # [GAGCTC] # 56 271 # # EcoRII: # [CCWGG] # 22 305 # # FatI: # [CATG] # 13 314 # # FokI: # [GGATG] # 123 204 # # HaeIII: # [GGCC] # 83 244 # # HgiAI*: # [GWGCWC] # 54 273 # # HgiJII*: # [GRGCYC] # 54 273 # # Hin4I: # [GAYNNNNNVTC] # 0 0 26 32 118 151 # # HinfI: # [GANTC] # 130 197 # # Hpy188I: # [TCNGA] # 66 261 # # Hpy99I: # [CGWCG] # 130 197 # # MaeI: # [CTAG] # 125 202 # # MaeIII: # [GTNAC] # 75 252 # # MboI: # [GATC] # 18 82 227 # # MboII: # [GAAGA] # 36 85 206 # # MseI: # [TTAA] # 29 69 229 # # NlaIII: # [CATG] # 9 318 # # SacI: # [GAGCTC] # 54 273 # # ScrFI: # [CCNGG] # 20 307 # # SduI: # [GDGCHC] # 54 273 # # SetI: # [ASST] # 27 37 54 209 # # SmlI: # [CTYRAG] # 55 272 # # TaqI: # [TCGA] # 101 226 # # TfiI: # [GAWTC] # 130 197 # # TsoI: # [TARCCA] # 69 120 138 # # Tsp4CI*: # [ACNGT] # 66 261 # # TspDTI: # [ATGAA] # 5 15 16 33 83 175 # # TspEI: # [AATT] # 30 69 70 158 # # TstI: # [CACNNNNNNTCC] # 32 35 260 # # XhoII: # [RGATCY] # 18 309 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 327 # Reported_sequences: 1 # Reported_hitcount: 76 #---------------------------------------