Restriction Fragments for GUT1/YHL032C



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The currently selected gene/sequence is :

GUT1/YHL032C on chromosome VIII from coordinates 38508 to 36379.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.28281.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 11 + MnlI CCTC 18 17 . . 13 18 + Ksp632I* CTCTTC 19 22 . . 17 22 + MmeI TCCRAC 42 40 . . 17 21 + Hpy188I TCNGA 19 18 . . 14 18 - MboII GAAGA 6 5 . . 38 43 + AclI AACGTT 39 41 . . 39 42 + TaiI ACGT 42 38 . . 39 42 + SetI ASST 42 38 . . 39 42 + MaeII ACGT 39 41 . . 41 45 + MaeIII GTNAC 40 45 . . 50 54 + Hpy188I TCNGA 52 51 . . 53 56 + DpnI GATC 54 54 . . 53 56 + BstKTI GATC 55 53 . . 53 56 + MboI GATC 52 56 . . 58 61 + CviJI RGCY 59 59 . . 59 64 + Cac8I GCNNGC 61 61 . . 62 67 + HaeII RGCGCY 66 62 . . 63 66 + Hin6I GCGC 63 65 . . 63 66 + GlaI GCGC 64 64 . . 63 66 + HhaI GCGC 65 63 . . 73 78 + SpeI ACTAGT 73 77 . . 74 77 + MaeI CTAG 74 76 . . 78 83 + AhaIII* TTTAAA 80 80 . . 79 82 + MseI TTAA 79 81 . . 94 97 + CviJI RGCY 95 95 . . 95 99 - BceAI ACGGC 80 82 . . 104 115 + BstXI CCANNNNNNTGG 111 107 . . 115 119 + MboII GAAGA 127 126 . . 128 132 + Tsp45I GTSAC 127 132 . . 128 132 + MaeIII GTNAC 127 132 . . 130 138 + Tth111I GACNNNGTC 133 134 . . 134 137 + TaiI ACGT 137 133 . . 134 137 + SetI ASST 137 133 . . 134 137 + MaeII ACGT 134 136 . . 138 142 + FauI CCCGC 146 148 . . 139 142 + AciI CCGC 139 141 . . 136 140 - BslFI GGGAC 121 125 . . 149 153 - BsrI ACTGG 149 147 . . 169 172 + SetI ASST 172 168 . . 170 173 + MnlI CCTC 180 179 . . 173 182 + MslI CAYNNNNRTG 177 177 . . 175 180 + Hpy178III* TCNNGA 176 178 . . 173 177 - FokI GGATG 159 163 . . 173 177 - BseGI GGATG 172 170 . . 180 185 + EcoT22I ATGCAT 184 180 . . 181 184 + CviRI* TGCA 182 182 . . 179 183 - SfaNI GCATC 169 173 . . 182 187 - BsmI GAATGC 182 180 . . 200 205 + NlaIV GGNNCC 202 202 . . 201 205 + BmgT120I GGNCC 202 203 . . 197 201 - BccI CCATC 191 192 . . 201 205 + AsuI* GGNCC 201 204 . . 202 205 + CviJI RGCY 203 203 . . 202 205 + HaeIII GGCC 203 203 . . 204 208 + BssKI CCNGG 203 208 . . 204 208 + BseBI CCWGG 205 206 . . 204 208 + ScrFI CCNGG 205 206 . . 204 208 + EcoRII CCWGG 203 208 . . 209 212 + TaiI ACGT 212 208 . . 209 212 + SetI ASST 212 208 . . 209 212 + MaeII ACGT 209 211 . . 225 228 + TspEI AATT 224 228 . . 230 235 + SspI AATATT 232 232 . . 244 248 + SfaNI GCATC 253 257 . . 239 244 - Eco57MI CTGRAG 224 222 . . 239 244 - Eco57I CTGAAG 224 222 . . 247 250 + TaqI TCGA 247 249 . . 249 253 - Hin4II* CCTTC 243 242 . . 271 274 + CviJI RGCY 272 272 . . 271 274 + HaeIII GGCC 272 272 . . 273 279 + SauI* CCTNAGG 274 277 . . 274 278 + DdeI CTNAG 274 277 . . 279 283 - BsmAI GTCTC 273 277 . . 284 287 + MnlI CCTC 294 293 . . 279 284 - Eco31I GGTCTC 273 277 . . 287 292 + SfeI* CTRYAG 287 291 . . 291 294 + CviJI RGCY 292 292 . . 294 299 + GsaI CCCAGC 298 294 . . 294 299 + BseYI CCCAGC 294 298 . . 297 300 + SetI ASST 300 296 . . 297 300 + CviJI RGCY 298 298 . . 297 300 + AluI AGCT 298 298 . . 300 305 + Hpy178III* TCNNGA 301 303 . . 299 304 - BsiI* CACGAG 299 303 . . 310 320 + BsiYI* CCNNNNNNNGG 316 313 . . 315 322 + SgrAI CRCCGGYG 316 320 . . 316 321 + Cfr10I RCCGGY 316 320 . . 317 320 + HpaII CCGG 317 319 . . 319 323 + HphI GGTGA 331 330 . . 320 324 + Tsp45I GTSAC 319 324 . . 320 324 + MaeIII GTNAC 319 324 . . 333 338 + NspBII* CMGCKG 335 335 . . 335 338 - AciI CCGC 335 337 . . 340 350 + FalI AAGNNNNNCTT 331 326 363 358 341 344 + CviJI RGCY 342 342 . . 344 348 + BccI CCATC 352 353 . . 340 350 - FalI AAGNNNNNCTT 363 358 331 326 353 358 + PstI CTGCAG 357 353 . . 353 358 + SfeI* CTRYAG 353 357 . . 354 357 + CviRI* TGCA 355 355 . . 361 364 + CviJI RGCY 362 362 . . 358 362 - Hin4II* CCTTC 352 351 . . 372 377 + Hpy178III* TCNNGA 373 375 . . 382 387 + ApoI RAATTY 382 386 . . 383 386 + TspEI AATT 382 386 . . 395 398 + TaqI TCGA 395 397 . . 397 400 - MnlI CCTC 390 389 . . 401 404 + TspEI AATT 400 404 . . 430 433 + TaiI ACGT 433 429 . . 430 433 + SetI ASST 433 429 . . 430 433 + MaeII ACGT 430 432 . . 441 451 + BsiYI* CCNNNNNNNGG 447 444 . . 442 452 + BsiYI* CCNNNNNNNGG 448 445 . . 448 452 + BssKI CCNGG 447 452 . . 448 451 + HpaII CCGG 448 450 . . 448 452 + ScrFI CCNGG 449 450 . . 448 452 + CauII* CCSGG 449 450 . . 465 469 + BccI CCATC 473 474 . . 469 472 + AciI CCGC 469 471 . . 466 470 - FokI GGATG 452 456 . . 466 470 - BseGI GGATG 465 463 . . 476 479 + TspEI AATT 475 479 . . 480 484 + BsrI ACTGG 485 483 . . 483 487 + HphI GGTGA 495 494 . . 484 489 + Hpy166II GTNNAC 486 486 . . 488 491 + TaiI ACGT 491 487 . . 488 491 + SetI ASST 491 487 . . 488 491 + MaeII ACGT 488 490 . . 489 493 + Hpy99I CGWCG 493 488 . . 506 509 + MnlI CCTC 516 515 . . 507 512 + SmlI CTYRAG 507 511 . . 507 512 - Bce83I* CTTGAG 492 490 . . 516 526 + MwoI GCNNNNNNNGC 522 519 . . 523 528 + PstI CTGCAG 527 523 . . 523 528 + SfeI* CTRYAG 523 527 . . 524 527 + CviRI* TGCA 525 525 . . 543 546 + TaiI ACGT 546 542 . . 543 546 + SetI ASST 546 542 . . 543 546 + MaeII ACGT 543 545 . . 543 548 + AflIII ACRYGT 543 547 . . 554 558 + Tsp4CI* ACNGT 556 555 . . 556 561 + Eco31I GGTCTC 562 566 . . 557 561 + BsmAI GTCTC 562 566 . . 564 567 + SetI ASST 567 563 . . 572 575 + SetI ASST 575 571 . . 579 584 + EcoT22I ATGCAT 583 579 . . 580 583 + CviRI* TGCA 581 581 . . 582 585 + FatI CATG 581 585 . . 582 585 + NlaIII CATG 585 581 . . 582 585 + CviAII CATG 582 584 . . 597 600 + FatI CATG 596 600 . . 597 600 + NlaIII CATG 600 596 . . 597 600 + CviAII CATG 597 599 . . 612 615 + TspEI AATT 611 615 . . 620 625 + NlaIV GGNNCC 622 622 . . 620 624 + BmgT120I GGNCC 621 622 . . 620 624 + AvaII GGWCC 620 623 . . 620 624 + AsuI* GGNCC 620 623 . . 623 627 + FauI CCCGC 631 633 . . 624 627 + AciI CCGC 624 626 . . 621 625 - BslFI GGGAC 606 610 . . 626 630 + TauI GCSGC 629 626 . . 626 630 + BlsI GCNGC 628 627 . . 626 630 + BisI GCNGC 627 628 . . 627 630 + AciI CCGC 627 629 . . 641 646 + MfeI CAATTG 641 645 . . 642 645 + TspEI AATT 641 645 . . 646 651 + Hpy166II GTNNAC 648 648 . . 646 651 + HpaI GTTAAC 648 648 . . 646 651 + HindII GTYRAC 648 648 . . 647 650 + MseI TTAA 647 649 . . 653 657 + Tsp4CI* ACNGT 655 654 . . 681 684 + DpnI GATC 682 682 . . 681 684 + BstKTI GATC 683 681 . . 681 690 + BsaBI GATNNNNATC 685 685 . . 681 684 + MboI GATC 680 684 . . 695 699 - BsmAI GTCTC 689 693 . . 713 716 + MaeI CTAG 713 715 . . 717 721 + Hpy99I CGWCG 721 716 . . 719 722 + TaqI TCGA 719 721 . . 716 720 - HgaI GACGC 705 710 . . 726 736 + MwoI GCNNNNNNNGC 732 729 . . 730 735 + PstI CTGCAG 734 730 . . 730 735 + SfeI* CTRYAG 730 734 . . 731 734 + CviRI* TGCA 732 732 . . 732 736 + BlsI GCNGC 734 733 . . 732 736 + BisI GCNGC 733 734 . . 732 736 + BbvI GCAGC 744 748 . . 732 736 + TseI GCWGC 732 735 . . 734 737 + SetI ASST 737 733 . . 734 737 + CviJI RGCY 735 735 . . 734 737 + AluI AGCT 735 735 . . 736 740 + DdeI CTNAG 736 739 . . 744 748 + MboII GAAGA 756 755 . . 744 749 + BbvII* GAAGAC 751 755 . . 748 752 + BsrI ACTGG 753 751 . . 758 763 - BsrDI GCAATG 757 755 . . 768 773 + BsaAI YACGTR 770 770 . . 769 772 + TaiI ACGT 772 768 . . 769 772 + SetI ASST 772 768 . . 769 772 + MaeII ACGT 769 771 . . 788 791 + SetI ASST 791 787 . . 788 791 + CviJI RGCY 789 789 . . 788 791 + AluI AGCT 789 789 . . 789 793 + BlsI GCNGC 791 790 . . 789 793 + BisI GCNGC 790 791 . . 789 793 + TseI GCWGC 789 792 . . 792 795 + Hin6I GCGC 792 794 . . 792 795 + GlaI GCGC 793 793 . . 792 795 + HhaI GCGC 794 792 . . 789 793 - BbvI GCAGC 776 780 . . 797 802 + NlaIV GGNNCC 799 799 . . 801 804 + MnlI CCTC 811 810 . . 803 806 + TaqI TCGA 803 805 . . 812 815 + CviJI RGCY 813 813 . . 814 817 + MnlI CCTC 824 823 . . 819 824 + Hpy166II GTNNAC 821 821 . . 821 824 + Csp6I GTAC 821 823 . . 821 824 + RsaI GTAC 822 822 . . 824 829 + StyI CCWWGG 824 828 . . 824 829 + SecI* CCNNGG 824 828 . . 835 840 + BseRI GAGGAG 850 848 . . 835 838 - MnlI CCTC 828 827 . . 845 848 + SetI ASST 848 844 . . 858 864 + TspRI CASTGNN 864 855 . . 859 863 + Tsp4CI* ACNGT 861 860 . . 862 867 + Hpy166II GTNNAC 864 864 . . 869 872 + FatI CATG 868 872 . . 869 872 + NlaIII CATG 872 868 . . 869 872 + CviAII CATG 869 871 . . 872 875 + CviJI RGCY 873 873 . . 884 887 + TspEI AATT 883 887 . . 886 889 + MseI TTAA 886 888 . . 910 914 + Hpy188I TCNGA 912 911 . . 914 917 + TaiI ACGT 917 913 . . 914 917 + SetI ASST 917 913 . . 914 917 + MaeII ACGT 914 916 . . 916 920 + MaeIII GTNAC 915 920 . . 917 922 + TsoI TARCCA 933 931 . . 928 933 + Hpy178III* TCNNGA 929 931 . . 934 938 + BsrI ACTGG 939 937 . . 943 947 + TspDTI ATGAA 958 956 . . 947 950 + SetI ASST 950 946 . . 948 951 + MnlI CCTC 958 957 . . 957 962 + AhaIII* TTTAAA 959 959 . . 958 961 + MseI TTAA 958 960 . . 963 966 + Csp6I GTAC 963 965 . . 963 966 + RsaI GTAC 964 964 . . 982 987 + ApoI RAATTY 982 986 . . 983 986 + TspEI AATT 982 986 . . 1004 1007 + SetI ASST 1007 1003 . . 1008 1012 + TfiI GAWTC 1008 1011 . . 1008 1012 + HinfI GANTC 1008 1011 . . 1013 1018 + NspI RCATGY 1017 1013 . . 1014 1017 + FatI CATG 1013 1017 . . 1014 1017 + NlaIII CATG 1017 1013 . . 1014 1017 + CviAII CATG 1014 1016 . . 1023 1026 + TspEI AATT 1022 1026 . . 1031 1034 + MnlI CCTC 1041 1040 . . 1046 1050 + Tsp4CI* ACNGT 1048 1047 . . 1048 1052 + HphI GGTGA 1060 1059 . . 1049 1053 + Tsp45I GTSAC 1048 1053 . . 1049 1053 + MaeIII GTNAC 1048 1053 . . 1062 1067 + Hpy178III* TCNNGA 1063 1065 . . 1058 1063 - BsmI GAATGC 1058 1056 . . 1082 1085 + SetI ASST 1085 1081 . . 1082 1085 + CviJI RGCY 1083 1083 . . 1082 1085 + AluI AGCT 1083 1083 . . 1090 1094 + TfiI GAWTC 1090 1093 . . 1090 1094 + HinfI GANTC 1090 1093 . . 1102 1106 + Tsp4CI* ACNGT 1104 1103 . . 1104 1109 + TatI WGTACW 1104 1108 . . 1104 1109 + ScaI AGTACT 1106 1106 . . 1105 1108 + Csp6I GTAC 1105 1107 . . 1105 1108 + RsaI GTAC 1106 1106 . . 1113 1118 + XhoII RGATCY 1113 1117 . . 1113 1118 + BglII AGATCT 1113 1117 . . 1114 1117 + DpnI GATC 1115 1115 . . 1114 1117 + BstKTI GATC 1116 1114 . . 1114 1117 + MboI GATC 1113 1117 . . 1117 1120 + MaeI CTAG 1117 1119 . . 1121 1126 + Hpy178III* TCNNGA 1122 1124 . . 1112 1122 - Hin4I GAYNNNNNVTC 1135 1130 1103 1098 1130 1133 + SetI ASST 1133 1129 . . 1130 1135 + BspMI ACCTGC 1139 1143 . . 1135 1145 + BsiYI* CCNNNNNNNGG 1141 1138 . . 1144 1147 + CviJI RGCY 1145 1145 . . 1145 1155 + MwoI GCNNNNNNNGC 1151 1148 . . 1154 1164 + MwoI GCNNNNNNNGC 1160 1157 . . 1163 1166 + Hin6I GCGC 1163 1165 . . 1163 1166 + GlaI GCGC 1164 1164 . . 1163 1166 + HhaI GCGC 1165 1163 . . 1165 1169 + SfaNI GCATC 1174 1178 . . 1169 1174 + DsaI* CCRYGG 1169 1173 . . 1169 1174 + StyI CCWWGG 1169 1173 . . 1169 1174 + NcoI CCATGG 1169 1173 . . 1169 1174 + SecI* CCNNGG 1169 1173 . . 1169 1179 + BsiYI* CCNNNNNNNGG 1175 1172 . . 1166 1170 - FokI GGATG 1152 1156 . . 1170 1173 + FatI CATG 1169 1173 . . 1170 1173 + NlaIII CATG 1173 1169 . . 1170 1173 + CviAII CATG 1170 1172 . . 1166 1170 - BseGI GGATG 1165 1163 . . 1195 1199 + BssKI CCNGG 1194 1199 . . 1195 1199 + ScrFI CCNGG 1196 1197 . . 1195 1199 + CauII* CCSGG 1196 1197 . . 1196 1199 + HpaII CCGG 1196 1198 . . 1201 1205 + BlsI GCNGC 1203 1202 . . 1201 1205 + BisI GCNGC 1202 1203 . . 1201 1205 + TseI GCWGC 1201 1204 . . 1203 1206 + CviRI* TGCA 1204 1204 . . 1201 1205 - BbvI GCAGC 1188 1192 . . 1210 1215 + TatI WGTACW 1210 1214 . . 1211 1214 + Csp6I GTAC 1211 1213 . . 1211 1214 + RsaI GTAC 1212 1212 . . 1219 1224 + KpnI GGTACC 1223 1219 . . 1219 1224 + NlaIV GGNNCC 1221 1221 . . 1219 1224 + HgiCI* GGYRCC 1219 1223 . . 1219 1224 + Acc65I GGTACC 1219 1223 . . 1220 1223 + Csp6I GTAC 1220 1222 . . 1220 1223 + RsaI GTAC 1221 1221 . . 1222 1227 + Cfr10I RCCGGY 1222 1226 . . 1222 1227 + BetI* WCCGGW 1222 1226 . . 1222 1227 + AgeI ACCGGT 1222 1226 . . 1223 1226 + HpaII CCGG 1223 1225 . . 1236 1240 + Tsp4CI* ACNGT 1238 1237 . . 1238 1243 + TatI WGTACW 1238 1242 . . 1238 1243 + Bsp1407I TGTACA 1238 1242 . . 1239 1242 + Csp6I GTAC 1239 1241 . . 1239 1242 + RsaI GTAC 1240 1240 . . 1249 1253 + BslFI GGGAC 1263 1267 . . 1249 1254 + NlaIV GGNNCC 1251 1251 . . 1250 1254 + BmgT120I GGNCC 1251 1252 . . 1250 1254 + AvaII GGWCC 1250 1253 . . 1250 1254 + AsuI* GGNCC 1250 1253 . . 1259 1262 + TspEI AATT 1258 1262 . . 1263 1266 + DpnI GATC 1264 1264 . . 1263 1266 + BstKTI GATC 1265 1263 . . 1263 1266 + MboI GATC 1262 1266 . . 1273 1276 + FatI CATG 1272 1276 . . 1273 1276 + NlaIII CATG 1276 1272 . . 1273 1276 + CviAII CATG 1273 1275 . . 1277 1280 + Hin6I GCGC 1277 1279 . . 1277 1280 + GlaI GCGC 1278 1278 . . 1277 1280 + HhaI GCGC 1279 1277 . . 1280 1286 + TspRI CASTGNN 1286 1277 . . 1287 1291 + HinfI GANTC 1287 1290 . . 1287 1291 - PleI GAGTC 1281 1282 . . 1287 1291 - MlyI GAGTC 1281 1281 . . 1291 1294 + MaeI CTAG 1291 1293 . . 1301 1306 + NlaIV GGNNCC 1303 1303 . . 1313 1316 + CviRI* TGCA 1314 1314 . . 1320 1323 + Csp6I GTAC 1320 1322 . . 1320 1323 + RsaI GTAC 1321 1321 . . 1322 1326 + Tsp4CI* ACNGT 1324 1323 . . 1326 1331 + BalI TGGCCA 1328 1328 . . 1326 1331 + CfrI YGGCCR 1326 1330 . . 1327 1330 + CviJI RGCY 1328 1328 . . 1327 1330 + HaeIII GGCC 1328 1328 . . 1340 1343 + TspEI AATT 1339 1343 . . 1346 1351 + Cac8I GCNNGC 1348 1348 . . 1349 1352 + CviJI RGCY 1350 1350 . . 1359 1362 + CviRI* TGCA 1360 1360 . . 1366 1369 - MnlI CCTC 1359 1358 . . 1369 1374 + NlaIV GGNNCC 1371 1371 . . 1374 1378 + Hpy99I CGWCG 1378 1373 . . 1372 1376 - TspGWI ACGGA 1362 1360 . . 1383 1386 + CviJI RGCY 1384 1384 . . 1395 1399 + BmgT120I GGNCC 1396 1397 . . 1395 1399 + AvaII GGWCC 1395 1398 . . 1395 1399 + AsuI* GGNCC 1395 1398 . . 1403 1406 + CviJI RGCY 1404 1404 . . 1406 1411 + BsaAI YACGTR 1408 1408 . . 1402 1407 - TsoI TARCCA 1392 1390 . . 1407 1410 + TaiI ACGT 1410 1406 . . 1407 1410 + SetI ASST 1410 1406 . . 1407 1410 + MaeII ACGT 1407 1409 . . 1414 1417 + TspEI AATT 1413 1417 . . 1425 1428 + DpnI GATC 1426 1426 . . 1425 1428 + BstKTI GATC 1427 1425 . . 1425 1428 + MboI GATC 1424 1428 . . 1426 1431 + ClaI ATCGAT 1427 1429 . . 1427 1430 + TaqI TCGA 1427 1429 . . 1435 1439 + Hpy188I TCNGA 1437 1436 . . 1440 1444 + FokI GGATG 1453 1457 . . 1440 1444 + BseGI GGATG 1446 1444 . . 1438 1441 - MnlI CCTC 1431 1430 . . 1445 1449 + Hpy188I TCNGA 1447 1446 . . 1446 1452 + RsrII CGGWCCG 1447 1450 . . 1447 1451 + BmgT120I GGNCC 1448 1449 . . 1447 1451 + AvaII GGWCC 1447 1450 . . 1447 1451 + AsuI* GGNCC 1447 1450 . . 1448 1453 + TaqII GACCGA 1464 1462 . . 1444 1449 - MmeI TCCRAC 1425 1423 . . 1455 1458 + CviRI* TGCA 1456 1456 . . 1456 1460 + SfaNI GCATC 1465 1469 . . 1462 1466 + Tsp4CI* ACNGT 1464 1463 . . 1464 1469 + NlaIV GGNNCC 1466 1466 . . 1467 1472 + Hpy178III* TCNNGA 1468 1470 . . 1468 1478 + BsiYI* CCNNNNNNNGG 1474 1471 . . 1471 1475 + TfiI GAWTC 1471 1474 . . 1471 1475 + HinfI GANTC 1471 1474 . . 1495 1499 + FauI CCCGC 1503 1505 . . 1492 1496 - BslFI GGGAC 1477 1481 . . 1496 1499 + AciI CCGC 1496 1498 . . 1507 1510 + CviJI RGCY 1508 1508 . . 1507 1510 + HaeIII GGCC 1508 1508 . . 1526 1532 + SanDI GGGWCCC 1527 1530 . . 1526 1532 + PpuMI RGGWCCY 1527 1530 . . 1526 1532 + DraII RGGNCCY 1527 1530 . . 1526 1530 + BslFI GGGAC 1540 1544 . . 1526 1531 + NlaIV GGNNCC 1528 1528 . . 1527 1532 + NlaIV GGNNCC 1529 1529 . . 1527 1531 + BmgT120I GGNCC 1528 1529 . . 1527 1531 + AvaII GGWCC 1527 1530 . . 1527 1531 + AsuI* GGNCC 1527 1530 . . 1534 1538 - SfaNI GCATC 1524 1528 . . 1542 1545 + CviJI RGCY 1543 1543 . . 1544 1555 + BstXI CCANNNNNNTGG 1551 1547 . . 1545 1554 + MslI CAYNNNNRTG 1549 1549 . . 1547 1557 + BsiYI* CCNNNNNNNGG 1553 1550 . . 1556 1560 + FokI GGATG 1569 1573 . . 1556 1560 + BseGI GGATG 1562 1560 . . 1560 1564 + BsmAI GTCTC 1565 1569 . . 1565 1568 + TspEI AATT 1564 1568 . . 1577 1580 + MnlI CCTC 1587 1586 . . 1588 1598 + MwoI GCNNNNNNNGC 1594 1591 . . 1589 1603 + XcmI CCANNNNNNNNNTGG 1596 1595 . . 1584 1589 - BtgZI GCGATG 1569 1573 . . 1593 1596 + SetI ASST 1596 1592 . . 1593 1596 + CviJI RGCY 1594 1594 . . 1593 1596 + AluI AGCT 1594 1594 . . 1594 1598 + BlsI GCNGC 1596 1595 . . 1594 1598 + BisI GCNGC 1595 1596 . . 1594 1598 + TseI GCWGC 1594 1597 . . 1598 1603 + DsaI* CCRYGG 1598 1602 . . 1598 1603 + SecI* CCNNGG 1598 1602 . . 1594 1598 - BbvI GCAGC 1581 1585 . . 1597 1601 - BceAI ACGGC 1582 1584 . . 1605 1608 + SetI ASST 1608 1604 . . 1603 1607 - Hin4II* CCTTC 1597 1596 . . 1620 1623 + CviJI RGCY 1621 1621 . . 1622 1626 + BssKI CCNGG 1621 1626 . . 1622 1626 + BseBI CCWGG 1623 1624 . . 1622 1627 + SecI* CCNNGG 1622 1626 . . 1622 1626 + ScrFI CCNGG 1623 1624 . . 1622 1626 + EcoRII CCWGG 1621 1626 . . 1626 1629 + CviJI RGCY 1627 1627 . . 1631 1636 + Hpy178III* TCNNGA 1632 1634 . . 1635 1639 - Hin4II* CCTTC 1629 1628 . . 1639 1644 + BsrDI GCAATG 1646 1644 . . 1648 1652 + Hpy188I TCNGA 1650 1649 . . 1651 1655 + HgaI GACGC 1660 1665 . . 1652 1657 + MluI ACGCGT 1652 1656 . . 1652 1657 + AflIII ACRYGT 1652 1656 . . 1653 1656 + FnuDII* CGCG 1654 1654 . . 1660 1664 + HphI GGTGA 1672 1671 . . 1665 1668 + SetI ASST 1668 1664 . . 1666 1671 + NlaIV GGNNCC 1668 1668 . . 1663 1667 - Hin4II* CCTTC 1657 1656 . . 1670 1680 + BsiYI* CCNNNNNNNGG 1676 1673 . . 1679 1683 + BslFI GGGAC 1693 1697 . . 1690 1693 - MnlI CCTC 1683 1682 . . 1694 1699 + ApoI RAATTY 1694 1698 . . 1695 1698 + TspEI AATT 1694 1698 . . 1699 1704 + MmeI TCCRAC 1724 1722 . . 1699 1703 + Hpy188I TCNGA 1701 1700 . . 1701 1705 + Hpy99I CGWCG 1705 1700 . . 1702 1707 + ZraI GACGTC 1704 1704 . . 1702 1707 + AcyI GRCGYC 1703 1705 . . 1702 1707 + AatII GACGTC 1706 1702 . . 1703 1706 + TaiI ACGT 1706 1702 . . 1703 1706 + SetI ASST 1706 1702 . . 1703 1706 + MaeII ACGT 1703 1705 . . 1705 1709 + Tsp45I GTSAC 1704 1709 . . 1705 1709 + MaeIII GTNAC 1704 1709 . . 1709 1714 + NdeI CATATG 1710 1712 . . 1712 1716 + TspDTI ATGAA 1727 1725 . . 1738 1743 + BtsI GCAGTG 1745 1743 . . 1739 1745 + TspRI CASTGNN 1745 1736 . . 1743 1747 + FokI GGATG 1756 1760 . . 1743 1747 + BseGI GGATG 1749 1747 . . 1744 1748 - BccI CCATC 1738 1739 . . 1751 1755 + FokI GGATG 1764 1768 . . 1751 1755 + BseGI GGATG 1757 1755 . . 1748 1751 - AciI CCGC 1748 1750 . . 1748 1752 - FauI CCCGC 1741 1743 . . 1756 1759 + TaqI TCGA 1756 1758 . . 1759 1762 + SetI ASST 1762 1758 . . 1758 1761 - MnlI CCTC 1751 1750 . . 1766 1770 + TspDTI ATGAA 1781 1779 . . 1773 1776 + FatI CATG 1772 1776 . . 1773 1776 + NlaIII CATG 1776 1772 . . 1773 1776 + CviAII CATG 1773 1775 . . 1775 1778 + CviRI* TGCA 1776 1776 . . 1778 1783 + ApoI RAATTY 1778 1782 . . 1779 1782 + TspEI AATT 1778 1782 . . 1785 1788 + CviJI RGCY 1786 1786 . . 1789 1794 + EcoRV GATATC 1791 1791 . . 1794 1798 + DdeI CTNAG 1794 1797 . . 1797 1800 + SetI ASST 1800 1796 . . 1797 1803 + PpuMI RGGWCCY 1798 1801 . . 1797 1803 + DraII RGGNCCY 1798 1801 . . 1798 1803 + NlaIV GGNNCC 1800 1800 . . 1798 1802 + BmgT120I GGNCC 1799 1800 . . 1798 1802 + AvaII GGWCC 1798 1801 . . 1798 1802 + AsuI* GGNCC 1798 1801 . . 1799 1803 - BslFI GGGAC 1784 1788 . . 1814 1818 + Hpy188I TCNGA 1816 1815 . . 1819 1822 + SetI ASST 1822 1818 . . 1820 1825 + Eco31I GGTCTC 1826 1830 . . 1817 1821 - Hin4II* CCTTC 1811 1810 . . 1821 1825 + BsmAI GTCTC 1826 1830 . . 1824 1829 + MmeI TCCRAC 1849 1847 . . 1824 1828 + Hpy188I TCNGA 1826 1825 . . 1829 1834 + NspBII* CMGCKG 1831 1831 . . 1831 1834 - AciI CCGC 1831 1833 . . 1838 1841 + Csp6I GTAC 1838 1840 . . 1838 1841 + RsaI GTAC 1839 1839 . . 1841 1851 + BsiYI* CCNNNNNNNGG 1847 1844 . . 1841 1844 + AciI CCGC 1841 1843 . . 1843 1853 + MwoI GCNNNNNNNGC 1849 1846 . . 1852 1862 + MwoI GCNNNNNNNGC 1858 1855 . . 1852 1862 + BstAPI GCANNNNNTGC 1858 1855 . . 1852 1856 + BlsI GCNGC 1854 1853 . . 1852 1856 + BisI GCNGC 1853 1854 . . 1852 1856 + BbvI GCAGC 1864 1868 . . 1852 1856 + TseI GCWGC 1852 1855 . . 1854 1857 + CviJI RGCY 1855 1855 . . 1855 1865 + MwoI GCNNNNNNNGC 1861 1858 . . 1860 1863 + CviRI* TGCA 1861 1861 . . 1861 1865 + BlsI GCNGC 1863 1862 . . 1861 1865 + BisI GCNGC 1862 1863 . . 1861 1865 + BbvI GCAGC 1873 1877 . . 1861 1865 + TseI GCWGC 1861 1864 . . 1857 1862 - BsrDI GCAATG 1856 1854 . . 1863 1866 + CviJI RGCY 1864 1864 . . 1864 1874 + MwoI GCNNNNNNNGC 1870 1867 . . 1864 1874 + BglI GCCNNNNNGGC 1870 1867 . . 1872 1875 + CviJI RGCY 1873 1873 . . 1865 1878 - AjuI GAANNNNNNNTTGG 1890 1885 1858 1853 1881 1885 + FokI GGATG 1894 1898 . . 1881 1885 + BseGI GGATG 1887 1885 . . 1885 1890 + Hpy166II GTNNAC 1887 1887 . . 1892 1897 + HaeII RGCGCY 1896 1892 . . 1893 1896 + Hin6I GCGC 1893 1895 . . 1893 1896 + GlaI GCGC 1894 1894 . . 1893 1896 + HhaI GCGC 1895 1893 . . 1907 1913 + PpuMI RGGWCCY 1908 1911 . . 1907 1913 + DraII RGGNCCY 1908 1911 . . 1908 1912 + BmgT120I GGNCC 1909 1910 . . 1908 1912 + AvaII GGWCC 1908 1911 . . 1908 1912 + AsuI* GGNCC 1908 1911 . . 1905 1909 - Hin4II* CCTTC 1899 1898 . . 1910 1913 + SetI ASST 1913 1909 . . 1922 1925 + MseI TTAA 1922 1924 . . 1969 1973 - HphI GGTGA 1961 1960 . . 1977 1980 + CviJI RGCY 1978 1978 . . 1975 1978 - MnlI CCTC 1968 1967 . . 1981 1985 - FokI GGATG 1967 1971 . . 1981 1985 - BseGI GGATG 1980 1978 . . 1988 1991 + SetI ASST 1991 1987 . . 1990 1995 + SmlI CTYRAG 1990 1994 . . 1990 1995 + AflII CTTAAG 1990 1994 . . 1991 1994 + MseI TTAA 1991 1993 . . 2000 2004 + Hpy188I TCNGA 2002 2001 . . 2008 2012 + TfiI GAWTC 2008 2011 . . 2008 2012 + HinfI GANTC 2008 2011 . . 2011 2016 + MmeI TCCRAC 2036 2034 . . 2011 2015 + Hpy188I TCNGA 2013 2012 . . 2013 2017 + Hpy99I CGWCG 2017 2012 . . 2017 2021 - SfaNI GCATC 2007 2011 . . 2057 2062 + DsaI* CCRYGG 2057 2061 . . 2057 2062 + SecI* CCNNGG 2057 2061 . . 2056 2060 - BceAI ACGGC 2041 2043 . . 2065 2070 + XhoII RGATCY 2065 2069 . . 2066 2069 + DpnI GATC 2067 2067 . . 2066 2069 + BstKTI GATC 2068 2066 . . 2066 2069 + MboI GATC 2065 2069 . . 2069 2079 + BsiYI* CCNNNNNNNGG 2075 2072 . . 2069 2079 + PflMI CCANNNNNTGG 2075 2072 . . 2066 2070 - BinI* GGATC 2060 2061 . . 2073 2076 + SetI ASST 2076 2072 . . 2078 2081 + CviJI RGCY 2079 2079 . . 2080 2085 + Eco57MI CTGRAG 2101 2099 . . 2080 2085 + Eco57I CTGAAG 2101 2099 . . 2082 2086 - Hin4II* CCTTC 2076 2075 . . 2094 2097 + SetI ASST 2097 2093 . . 2095 2099 + HphI GGTGA 2107 2106 . . 2092 2096 - Hin4II* CCTTC 2086 2085 . . 2096 2101 + Hpy166II GTNNAC 2098 2098 . . 2104 2113 + XmnI GAANNNNTTC 2108 2108 . . 2108 2111 + SetI ASST 2111 2107 . . 2112 2117 + XbaI TCTAGA 2112 2116 . . 2112 2117 + Hpy178III* TCNNGA 2113 2115 . . 2113 2116 + MaeI CTAG 2113 2115 . . #--------------------------------------- # # # AatII: # [GACGTC] # 424 1706 # # Acc65I: # [GGTACC] # 911 1219 # # AciI: # [CCGC] # 3 10 83 134 139 155 # 196 252 289 869 # # AclI: # [AACGTT] # 39 2091 # # AcyI: # [GRCGYC] # 427 1703 # # AflII: # [CTTAAG] # 140 1990 # # AflIII: # [ACRYGT] # 478 543 1109 # # AgeI: # [ACCGGT] # 908 1222 # # AhaIII*: # [TTTAAA] # 80 879 1171 # # AjuI: # [GAANNNNNNNTTGG] # 32 240 1858 # # AluI: # [AGCT] # 54 294 298 437 511 536 # # ApoI: # [RAATTY] # 84 352 382 600 712 # # AsuI*: # [GGNCC] # 52 80 110 145 201 222 # 271 419 630 # # AvaII: # [GGWCC] # 52 80 110 145 222 271 # 620 630 # # BalI: # [TGGCCA] # 802 1328 # # BbvI: # [GCAGC] # 9 32 257 283 393 412 # 744 # # BbvII*: # [GAAGAC] # 751 1379 # # BccI: # [CCATC] # 121 161 191 392 1265 # # Bce83I*: # [CTTGAG] # 492 1638 # # BceAI: # [ACGGC] # 80 89 459 1502 # # BetI*: # [WCCGGW] # 908 1222 # # BglI: # [GCCNNNNNGGC] # 260 1870 # # BglII: # [AGATCT] # 1017 1113 # # BinI*: # [GGATC] # 70 2060 # # BisI: # [GCNGC] # 9 57 106 258 268 393 # 412 627 # # BlsI: # [GCNGC] # 9 57 106 258 267 393 # 412 628 # # BmgT120I: # [GGNCC] # 52 80 110 145 202 221 # 271 419 630 # # BsaAI: # [YACGTR] # 638 722 770 # # BsaBI: # [GATNNNNATC] # 685 1445 # # BseBI: # [CCWGG] # 205 507 1418 # # BseGI: # [GGATG] # 8 93 116 130 150 172 # 187 281 293 700 # # BseRI: # [GAGGAG] # 850 1280 # # BseYI: # [CCCAGC] # 294 1836 # # BsiI*: # [CACGAG] # 299 1831 # # BsiYI*: # [CCNNNNNNNGG] # 1 34 55 79 123 131 # 171 228 299 316 693 # # BslFI: # [GGGAC] # 63 91 121 153 214 346 # 485 657 # # BsmAI: # [GTCTC] # 127 261 273 289 304 876 # # BsmI: # [GAATGC] # 182 876 1072 # # Bsp1407I: # [TGTACA] # 892 1238 # # BspMI: # [ACCTGC] # 991 1139 # # BsrDI: # [GCAATG] # 210 274 757 889 # # BsrI: # [ACTGG] # 149 186 268 336 1191 # # BssKI: # [CCNGG] # 203 244 427 509 747 # # BstAPI: # [GCANNNNNTGC] # 272 1858 # # BstKTI: # [GATC] # 55 62 149 162 433 628 # 641 # # BstXI: # [CCANNNNNNTGG] # 111 579 1440 # # BtgZI: # [GCGATG] # 561 1569 # # BtsI: # [GCAGTG] # 385 1745 # # Cac8I: # [GCNNGC] # 61 782 1287 # # CauII*: # [CCSGG] # 449 747 934 # # Cfr10I: # [RCCGGY] # 316 906 908 # # CfrI: # [YGGCCR] # 804 1326 # # ClaI: # [ATCGAT] # 703 1427 # # Csp6I: # [GTAC] # 9 19 81 106 142 142 # 292 518 821 # # CviAII: # [CATG] # 15 103 145 156 272 357 # 500 582 # # CviJI: # [RGCY] # 6 6 9 9 20 20 # 20 22 24 27 34 35 # 36 44 51 51 54 59 # 60 62 69 69 101 104 # 105 108 159 183 210 373 # # CviRI*: # [TGCA] # 46 56 85 96 110 151 # 170 173 182 269 320 472 # # DdeI: # [CTNAG] # 274 336 462 1058 # # DpnI: # [GATC] # 54 63 149 162 433 628 # 641 # # DraII: # [RGGNCCY] # 110 222 271 1527 # # DsaI*: # [CCRYGG] # 73 429 459 1169 # # Eco31I: # [GGTCTC] # 273 289 304 1264 # # Eco57I: # [CTGAAG] # 29 224 1877 # # Eco57MI: # [CTGRAG] # 29 224 1877 # # EcoRII: # [CCWGG] # 203 509 1418 # # EcoRV: # [GATATC] # 339 1791 # # EcoT22I: # [ATGCAT] # 184 399 1547 # # FalI: # [AAGNNNNNCTT] # 0 0 32 331 1767 # # FatI: # [CATG] # 15 103 145 156 272 358 # 500 581 # # FauI: # [CCCGC] # 146 238 389 485 872 # # FnuDII*: # [CGCG] # 476 1654 # # FokI: # [GGATG] # 8 73 116 130 159 163 # 187 293 301 700 # # GlaI: # [GCGC] # 64 114 236 371 616 729 # # GsaI: # [CCCAGC] # 298 1832 # # HaeII: # [RGCGCY] # 66 234 1830 # # HaeIII: # [GGCC] # 69 180 203 622 1056 # # HgaI: # [GACGC] # 470 705 955 # # HgiCI*: # [GGYRCC] # 911 1219 # # HhaI: # [GCGC] # 65 114 235 371 616 729 # # Hin4I: # [GAYNNNNNVTC] # 32 995 1103 # # Hin4II*: # [CCTTC] # 10 28 32 44 88 109 # 154 177 243 1245 # # Hin6I: # [GCGC] # 63 114 237 371 616 729 # # HindII: # [GTYRAC] # 648 1482 # # HinfI: # [GANTC] # 82 122 184 197 537 1008 # # HpaI: # [GTTAAC] # 648 1482 # # HpaII: # [CCGG] # 27 131 317 748 907 # # HphI: # [GGTGA] # 23 146 164 289 331 565 # 612 # # Hpy166II: # [GTNNAC] # 32 43 162 173 211 486 # 1023 # # Hpy178III*: # [TCNNGA] # 17 59 72 125 134 164 # 176 346 481 556 # # Hpy188I: # [TCNGA] # 10 10 11 19 33 51 # 115 117 176 203 525 860 # # Hpy99I: # [CGWCG] # 113 228 312 327 493 657 # # KpnI: # [GGTACC] # 907 1223 # # Ksp632I*: # [CTCTTC] # 19 2111 # # MaeI: # [CTAG] # 17 74 174 404 639 822 # # MaeII: # [ACGT] # 39 55 58 75 95 145 # 221 226 296 427 493 # # MaeIII: # [GTNAC] # 40 87 133 192 426 596 # 656 # # MboI: # [GATC] # 52 65 149 162 433 628 # 641 # # MboII: # [GAAGA] # 6 121 629 1374 # # MfeI: # [CAATTG] # 641 1489 # # MluI: # [ACGCGT] # 478 1652 # # MlyI: # [GAGTC] # 849 1281 # # MmeI: # [TCCRAC] # 42 94 125 187 299 1383 # # MnlI: # [CCTC] # 4 13 18 68 72 83 # 96 96 114 126 130 156 # 162 162 217 295 318 # # MseI: # [TTAA] # 69 72 79 139 239 568 # 964 # # MslI: # [CAYNNNNRTG] # 177 581 1372 # # MwoI: # [GCNNNNNNNGC] # 3 9 9 9 210 255 # 260 419 434 522 # # NcoI: # [CCATGG] # 961 1169 # # NdeI: # [CATATG] # 420 1710 # # NlaIII: # [CATG] # 15 103 145 156 272 354 # 500 585 # # NlaIV: # [GGNNCC] # 1 30 52 62 68 95 # 132 139 177 202 330 420 # 422 # # NspBII*: # [CMGCKG] # 299 335 1496 # # NspI: # [RCATGY] # 1017 1113 # # PflMI: # [CCANNNNNTGG] # 55 2075 # # PleI: # [GAGTC] # 849 1281 # # PpuMI: # [RGGWCCY] # 110 222 271 1527 # # PstI: # [CTGCAG] # 170 207 357 1396 # # RsaI: # [GTAC] # 9 19 81 106 142 142 # 291 518 822 # # RsrII: # [CGGWCCG] # 683 1447 # # SanDI: # [GGGWCCC] # 603 1527 # # SauI*: # [CCTNAGG] # 274 1856 # # ScaI: # [AGTACT] # 1024 1106 # # ScrFI: # [CCNGG] # 205 244 427 507 747 # # SecI*: # [CCNNGG] # 24 73 345 429 435 824 # # SetI: # [ASST] # 8 12 14 19 19 21 # 21 22 33 35 35 38 # 38 40 42 48 55 56 # 57 57 58 60 69 78 # 78 85 88 91 95 133 # 162 186 277 # # SfaNI: # [GCATC] # 59 84 123 169 291 483 # 921 # # SfeI*: # [CTRYAG] # 66 170 207 287 1400 # # SgrAI: # [CRCCGGYG] # 316 1814 # # SmlI: # [CTYRAG] # 140 507 1483 # # SpeI: # [ACTAGT] # 73 2057 # # SspI: # [AATATT] # 232 1898 # # StyI: # [CCWWGG] # 345 824 961 # # TaiI: # [ACGT] # 42 55 58 75 95 145 # 221 226 296 424 493 # # TaqI: # [TCGA] # 84 148 247 324 329 374 # 624 # # TaqII: # [GACCGA] # 666 1464 # # TatI: # [WGTACW] # 28 106 892 1104 # # TauI: # [GCSGC] # 629 1501 # # TfiI: # [GAWTC] # 82 122 381 537 1008 # # TseI: # [GCWGC] # 9 57 258 269 393 412 # 732 # # TsoI: # [TARCCA] # 459 738 933 # # Tsp45I: # [GTSAC] # 127 192 426 656 729 # # Tsp4CI*: # [ACNGT] # 56 86 99 134 140 187 # 206 556 666 # # TspDTI: # [ATGAA] # 54 349 769 958 # # TspEI: # [AATT] # 18 30 40 74 75 81 # 84 99 130 136 151 158 # 224 236 242 352 # # TspGWI: # [ACGGA] # 768 1362 # # TspRI: # [CASTGNN] # 385 422 459 864 # # Tth111I: # [GACNNNGTC] # 133 1997 # # XbaI: # [TCTAGA] # 18 2112 # # XcmI: # [CCANNNNNNNNNTGG] # 534 1596 # # XhoII: # [RGATCY] # 65 952 1113 # # XmnI: # [GAANNNNTTC] # 22 2108 # # ZraI: # [GACGTC] # 426 1704 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2130 # Reported_sequences: 1 # Reported_hitcount: 596 #---------------------------------------