Restriction Fragments for GOS1/YHL031C



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The currently selected gene/sequence is :

GOS1/YHL031C on chromosome VIII from coordinates 39486 to 38815.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.12892.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + SacI GAGCTC 7 3 . . 3 8 + Ecl136II GAGCTC 5 5 . . 3 8 + SduI GDGCHC 7 3 . . 3 8 + HgiJII* GRGCYC 7 3 . . 3 8 + HgiAI* GWGCWC 7 3 . . 4 7 + SetI ASST 7 3 . . 4 7 + CviJI RGCY 5 5 . . 4 7 + AluI AGCT 5 5 . . 12 16 + Tsp4CI* ACNGT 14 13 . . 22 26 + Tsp45I GTSAC 21 26 . . 22 26 + MaeIII GTNAC 21 26 . . 23 27 - HphI GGTGA 15 14 . . 39 42 + CviJI RGCY 40 40 . . 39 42 + HaeIII GGCC 40 40 . . 48 53 + XbaI TCTAGA 48 52 . . 48 53 + Hpy178III* TCNNGA 49 51 . . 49 52 + MaeI CTAG 49 51 . . 61 65 + TspGWI ACGGA 76 74 . . 64 68 + PleI GAGTC 72 73 . . 64 68 + MlyI GAGTC 73 73 . . 64 68 + HinfI GANTC 64 67 . . 66 70 + Tsp45I GTSAC 65 70 . . 66 70 + MaeIII GTNAC 65 70 . . 80 85 + TatI WGTACW 80 84 . . 80 85 + ScaI AGTACT 82 82 . . 81 84 + Csp6I GTAC 81 83 . . 81 84 + RsaI GTAC 82 82 . . 93 96 + FnuDII* CGCG 94 94 . . 94 97 + Hin6I GCGC 94 96 . . 94 97 + GlaI GCGC 95 95 . . 94 97 + HhaI GCGC 96 94 . . 103 108 + SpeI ACTAGT 103 107 . . 104 107 + MaeI CTAG 104 106 . . 109 113 + Hpy188I TCNGA 111 110 . . 118 122 + BsrI ACTGG 123 121 . . 120 125 + BalI TGGCCA 122 122 . . 120 125 + CfrI YGGCCR 120 124 . . 121 124 + CviJI RGCY 122 122 . . 121 124 + HaeIII GGCC 122 122 . . 132 136 + MboII GAAGA 144 143 . . 147 152 - MmeI TCCRAC 128 126 . . 151 154 - MnlI CCTC 144 143 . . 155 159 + TfiI GAWTC 155 158 . . 155 159 + HinfI GANTC 155 158 . . 159 163 + DdeI CTNAG 159 162 . . 163 166 + CviJI RGCY 164 164 . . 163 166 + HaeIII GGCC 164 164 . . 165 175 + BsiYI* CCNNNNNNNGG 171 168 . . 168 172 - BsmAI GTCTC 162 166 . . 174 178 + FokI GGATG 187 191 . . 174 178 + BseGI GGATG 180 178 . . 181 186 + ClaI ATCGAT 182 184 . . 182 185 + TaqI TCGA 182 184 . . 184 188 + TfiI GAWTC 184 187 . . 184 188 + HinfI GANTC 184 187 . . 186 190 - TspDTI ATGAA 176 174 . . 190 193 + MseI TTAA 190 192 . . 205 209 + TfiI GAWTC 205 208 . . 205 209 + HinfI GANTC 205 208 . . 213 217 + BssKI CCNGG 212 217 . . 213 217 + ScrFI CCNGG 214 215 . . 213 217 + CauII* CCSGG 214 215 . . 214 217 + HpaII CCGG 214 216 . . 217 227 + MwoI GCNNNNNNNGC 223 220 . . 224 227 + AciI CCGC 224 226 . . 227 230 + MnlI CCTC 237 236 . . 223 228 - EciI GGCGGA 213 211 . . 232 237 + HindIII AAGCTT 232 236 . . 233 236 + SetI ASST 236 232 . . 233 236 + CviJI RGCY 234 234 . . 233 236 + AluI AGCT 234 234 . . 245 248 + CviRI* TGCA 246 246 . . 244 249 - BsgI GTGCAG 229 227 . . 249 259 + BsiYI* CCNNNNNNNGG 255 252 . . 249 252 + AciI CCGC 249 251 . . 256 266 + FalI AAGNNNNNCTT 247 242 279 274 260 265 + XhoII RGATCY 260 264 . . 260 265 + BglII AGATCT 260 264 . . 261 264 + DpnI GATC 262 262 . . 261 264 + BstKTI GATC 263 261 . . 261 264 + MboI GATC 260 264 . . 256 266 - FalI AAGNNNNNCTT 279 274 247 242 274 280 + TspRI CASTGNN 280 271 . . 275 279 + BsrI ACTGG 280 278 . . 279 283 + MboII GAAGA 291 290 . . 279 288 + XmnI GAANNNNTTC 283 283 . . 283 286 + SetI ASST 286 282 . . 283 286 + CviJI RGCY 284 284 . . 283 286 + AluI AGCT 284 284 . . 279 284 - Ksp632I* CTCTTC 274 277 . . 279 285 - SapI GCTCTTC 274 277 . . 288 291 + AciI CCGC 288 290 . . 298 301 + SetI ASST 301 297 . . 298 304 + PpuMI RGGWCCY 299 302 . . 298 304 + DraII RGGNCCY 299 302 . . 299 303 + BmgT120I GGNCC 300 301 . . 299 303 + AvaII GGWCC 299 302 . . 299 303 + AsuI* GGNCC 299 302 . . 302 305 + MnlI CCTC 312 311 . . 305 309 + BccI CCATC 313 314 . . 306 310 - FokI GGATG 292 296 . . 306 310 - BseGI GGATG 305 303 . . 318 321 + Hin6I GCGC 318 320 . . 318 321 + GlaI GCGC 319 319 . . 318 321 + HhaI GCGC 320 318 . . 348 352 + MboII GAAGA 360 359 . . 364 367 + TspEI AATT 363 367 . . 373 377 + TspGWI ACGGA 388 386 . . 376 380 + HgaI GACGC 385 390 . . 378 381 + FnuDII* CGCG 379 379 . . 379 382 + Hin6I GCGC 379 381 . . 379 382 + GlaI GCGC 380 380 . . 379 382 + HhaI GCGC 381 379 . . 381 386 + Cac8I GCNNGC 383 383 . . 384 387 + SetI ASST 387 383 . . 384 387 + CviJI RGCY 385 385 . . 384 387 + AluI AGCT 385 385 . . 389 394 + SfeI* CTRYAG 389 393 . . 396 400 + FokI GGATG 409 413 . . 396 400 + BseGI GGATG 402 400 . . 397 401 - SfaNI GCATC 387 391 . . 407 412 + TatI WGTACW 407 411 . . 408 411 + Csp6I GTAC 408 410 . . 408 411 + RsaI GTAC 409 409 . . 414 418 + Hpy188I TCNGA 416 415 . . 431 435 + TfiI GAWTC 431 434 . . 431 435 + HinfI GANTC 431 434 . . 433 438 + ClaI ATCGAT 434 436 . . 434 437 + TaqI TCGA 434 436 . . 436 439 + DpnI GATC 437 437 . . 436 439 + BstKTI GATC 438 436 . . 436 439 + MboI GATC 435 439 . . 442 447 + MmeI TCCRAC 467 465 . . 449 452 + TaiI ACGT 452 448 . . 449 452 + SetI ASST 452 448 . . 449 452 + MaeII ACGT 449 451 . . 454 459 + SalI GTCGAC 454 458 . . 454 459 + Hpy166II GTNNAC 456 456 . . 454 459 + HindII GTYRAC 456 456 . . 454 459 + AccI GTMKAC 455 457 . . 455 458 + TaqI TCGA 455 457 . . 456 461 + McrI* CGRYCG 459 457 . . 458 462 + Tsp4CI* ACNGT 460 459 . . 460 465 + Esp3I CGTCTC 466 470 . . 461 465 + BsmAI GTCTC 466 470 . . 470 480 + BsiYI* CCNNNNNNNGG 476 473 . . 471 481 + BsiYI* CCNNNNNNNGG 477 474 . . 474 477 + CviJI RGCY 475 475 . . 476 480 + BssKI CCNGG 475 480 . . 476 480 + BseBI CCWGG 477 478 . . 476 481 + SecI* CCNNGG 476 480 . . 476 480 + ScrFI CCNGG 477 478 . . 476 480 + EcoRII CCWGG 475 480 . . 483 487 + HinfI GANTC 483 486 . . 483 491 + Tth111I GACNNNGTC 486 487 . . 481 485 - BsmAI GTCTC 475 479 . . 483 487 - PleI GAGTC 477 478 . . 483 487 - MlyI GAGTC 477 477 . . 487 492 + McrI* CGRYCG 490 488 . . 480 490 - BsaXI ACNNNNNCTCC 502 499 472 469 486 491 - TaqII GACCGA 476 474 . . 481 491 - Hin4I GAYNNNNNVTC 504 499 472 467 512 515 + TaiI ACGT 515 511 . . 512 515 + SetI ASST 515 511 . . 512 515 + MaeII ACGT 512 514 . . 523 527 + BceAI ACGGC 539 541 . . 536 539 + SetI ASST 539 535 . . 542 545 + CviRI* TGCA 543 543 . . 561 567 + SexAI ACCWGGT 561 566 . . 562 566 + BssKI CCNGG 561 566 . . 562 566 + BseBI CCWGG 563 564 . . 562 566 + ScrFI CCNGG 563 564 . . 562 566 + EcoRII CCWGG 561 566 . . 564 567 + SetI ASST 567 563 . . 575 578 + SetI ASST 578 574 . . 575 578 + CviJI RGCY 576 576 . . 575 578 + AluI AGCT 576 576 . . 583 587 + TspDTI ATGAA 598 596 . . 585 589 + MboII GAAGA 597 596 . . 590 593 + MseI TTAA 590 592 . . 594 599 + BsiI* CACGAG 594 598 . . 603 607 + MboII GAAGA 615 614 . . 599 603 - Hin4II* CCTTC 593 592 . . 611 616 + MluI ACGCGT 611 615 . . 611 616 + AflIII ACRYGT 611 615 . . 612 615 + FnuDII* CGCG 613 613 . . 623 628 + BalI TGGCCA 625 625 . . 623 628 + CfrI YGGCCR 623 627 . . 624 627 + CviJI RGCY 625 625 . . 624 627 + HaeIII GGCC 625 625 . . 632 637 + TsoI TARCCA 648 646 . . 644 649 + Hpy166II GTNNAC 646 646 . . 644 649 + BssNAI GTATAC 646 646 . . 644 649 + AccI GTMKAC 645 647 . . 648 652 + Tsp4CI* ACNGT 650 649 . . 665 668 + FatI CATG 664 668 . . 665 668 + NlaIII CATG 668 664 . . 665 668 + CviAII CATG 665 667 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 27 190 455 # # AciI: # [CCGC] # 25 39 224 384 # # AflIII: # [ACRYGT] # 61 611 # # AluI: # [AGCT] # 5 50 96 101 191 229 # # AsuI*: # [GGNCC] # 299 373 # # AvaII: # [GGWCC] # 299 373 # # BalI: # [TGGCCA] # 47 122 503 # # BccI: # [CCATC] # 313 359 # # BceAI: # [ACGGC] # 133 539 # # BglII: # [AGATCT] # 260 412 # # BmgT120I: # [GGNCC] # 300 372 # # BsaXI: # [ACNNNNNCTCC] # 30 170 472 # # BseBI: # [CCWGG] # 86 109 477 # # BseGI: # [GGATG] # 97 125 180 270 # # BsgI: # [GTGCAG] # 229 443 # # BsiI*: # [CACGAG] # 78 594 # # BsiYI*: # [CCNNNNNNNGG] # 1 84 171 195 221 # # BsmAI: # [GTCTC] # 9 162 197 304 # # BsrI: # [ACTGG] # 123 157 392 # # BssKI: # [CCNGG] # 86 111 212 263 # # BssNAI: # [GTATAC] # 26 646 # # BstKTI: # [GATC] # 175 234 263 # # Cac8I: # [GCNNGC] # 289 383 # # CauII*: # [CCSGG] # 214 458 # # CfrI: # [YGGCCR] # 49 120 503 # # ClaI: # [ATCGAT] # 182 238 252 # # Csp6I: # [GTAC] # 81 264 327 # # CviAII: # [CATG] # 7 665 # # CviJI: # [RGCY] # 5 35 42 47 49 50 # 70 82 90 101 101 # # CviRI*: # [TGCA] # 129 246 297 # # DdeI: # [CTNAG] # 159 513 # # DpnI: # [GATC] # 175 235 262 # # DraII: # [RGGNCCY] # 299 373 # # EciI: # [GGCGGA] # 213 459 # # Ecl136II: # [GAGCTC] # 5 667 # # EcoRII: # [CCWGG] # 86 111 475 # # Esp3I: # [CGTCTC] # 206 466 # # FalI: # [AAGNNNNNCTT] # 0 0 32 247 393 # # FatI: # [CATG] # 8 664 # # FnuDII*: # [CGCG] # 59 94 234 285 # # FokI: # [GGATG] # 105 117 187 263 # # GlaI: # [GCGC] # 61 95 224 292 # # HaeIII: # [GGCC] # 40 42 47 82 461 # # HgaI: # [GACGC] # 287 385 # # HgiAI*: # [GWGCWC] # 7 665 # # HgiJII*: # [GRGCYC] # 7 665 # # HhaI: # [GCGC] # 61 96 224 291 # # Hin4I: # [GAYNNNNNVTC] # 32 168 472 # # Hin4II*: # [CCTTC] # 79 593 # # Hin6I: # [GCGC] # 61 94 224 293 # # HindII: # [GTYRAC] # 216 456 # # HindIII: # [AAGCTT] # 232 440 # # HinfI: # [GANTC] # 21 29 52 64 91 189 # 226 # # HpaII: # [CCGG] # 214 458 # # HphI: # [GGTGA] # 15 657 # # Hpy166II: # [GTNNAC] # 26 190 456 # # Hpy178III*: # [TCNNGA] # 49 623 # # Hpy188I: # [TCNGA] # 111 256 305 # # Ksp632I*: # [CTCTTC] # 274 398 # # MaeI: # [CTAG] # 49 55 568 # # MaeII: # [ACGT] # 63 160 449 # # MaeIII: # [GTNAC] # 21 44 607 # # MboI: # [GATC] # 175 237 260 # # MboII: # [GAAGA] # 18 57 69 144 147 237 # # McrI*: # [CGRYCG] # 31 182 459 # # MluI: # [ACGCGT] # 61 611 # # MlyI: # [GAGTC] # 73 195 404 # # MmeI: # [TCCRAC] # 128 205 339 # # MnlI: # [CCTC] # 75 93 144 360 # # MseI: # [TTAA] # 82 190 400 # # MwoI: # [GCNNNNNNNGC] # 223 449 # # NlaIII: # [CATG] # 4 668 # # PleI: # [GAGTC] # 72 195 405 # # PpuMI: # [RGGWCCY] # 299 373 # # RsaI: # [GTAC] # 82 263 327 # # SacI: # [GAGCTC] # 7 665 # # SalI: # [GTCGAC] # 218 454 # # SapI: # [GCTCTTC] # 274 398 # # ScaI: # [AGTACT] # 82 590 # # ScrFI: # [CCNGG] # 86 109 214 263 # # SduI: # [GDGCHC] # 7 665 # # SecI*: # [CCNNGG] # 196 476 # # SetI: # [ASST] # 7 11 15 24 28 50 # 63 65 86 94 229 # # SexAI: # [ACCWGGT] # 111 561 # # SfaNI: # [GCATC] # 285 387 # # SfeI*: # [CTRYAG] # 283 389 # # SpeI: # [ACTAGT] # 103 569 # # TaiI: # [ACGT] # 63 157 452 # # TaqI: # [TCGA] # 21 182 217 252 # # TaqII: # [GACCGA] # 196 476 # # TatI: # [WGTACW] # 80 265 327 # # TfiI: # [GAWTC] # 21 29 155 226 241 # # TsoI: # [TARCCA] # 24 648 # # Tsp45I: # [GTSAC] # 21 44 607 # # Tsp4CI*: # [ACNGT] # 14 22 190 446 # # TspDTI: # [ATGAA] # 74 176 422 # # TspEI: # [AATT] # 309 363 # # TspGWI: # [ACGGA] # 76 284 312 # # TspRI: # [CASTGNN] # 280 392 # # Tth111I: # [GACNNNGTC] # 186 486 # # XbaI: # [TCTAGA] # 48 624 # # XhoII: # [RGATCY] # 260 412 # # XmnI: # [GAANNNNTTC] # 283 389 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 672 # Reported_sequences: 1 # Reported_hitcount: 198 #---------------------------------------