Restriction Fragments for YHL009W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YHL009W-A on chromosome VIII from coordinates 85909 to 87150.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.7303.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 18 + BsiYI* CCNNNNNNNGG 14 11 . . 9 19 + BsiYI* CCNNNNNNNGG 15 12 . . 11 17 + TspRI CASTGNN 17 8 . . 10 14 - BsrI ACTGG 10 8 . . 9 14 - BfiI ACTGGG 4 3 . . 15 18 - MnlI CCTC 8 7 . . 18 22 + FokI GGATG 31 35 . . 18 22 + BseGI GGATG 24 22 . . 20 24 + TspDTI ATGAA 35 33 . . 55 58 + Hin6I GCGC 55 57 . . 55 58 + GlaI GCGC 56 56 . . 55 58 + HhaI GCGC 57 55 . . 56 59 + FnuDII* CGCG 57 57 . . 60 64 + TfiI GAWTC 60 63 . . 60 64 + HinfI GANTC 60 63 . . 57 60 - AciI CCGC 57 59 . . 67 70 + TaqI TCGA 67 69 . . 88 92 + Tsp4CI* ACNGT 90 89 . . 92 96 + Hpy188I TCNGA 94 93 . . 99 103 + HgaI GACGC 108 113 . . 99 104 + AcyI GRCGYC 100 102 . . 103 107 + BccI CCATC 111 112 . . 112 115 + MseI TTAA 112 114 . . 116 125 + XmnI GAANNNNTTC 120 120 . . 128 131 + MseI TTAA 128 130 . . 134 138 + TspDTI ATGAA 149 147 . . 140 143 + SetI ASST 143 139 . . 141 145 + HphI GGTGA 153 152 . . 163 166 + MseI TTAA 163 165 . . 169 172 + SetI ASST 172 168 . . 172 177 + AhaIII* TTTAAA 174 174 . . 173 176 + MseI TTAA 173 175 . . 192 197 + AhaIII* TTTAAA 194 194 . . 193 196 + MseI TTAA 193 195 . . 202 206 + MboII GAAGA 214 213 . . 202 207 - Ksp632I* CTCTTC 197 200 . . 205 208 - MnlI CCTC 198 197 . . 217 221 + Hpy188I TCNGA 219 218 . . 218 222 + DdeI CTNAG 218 221 . . 218 222 - BspCNI CTCAG 210 208 . . 218 222 - BseMII CTCAG 209 207 . . 220 224 - BsmAI GTCTC 214 218 . . 225 236 + BdaI TGANNNNNNTCA 214 212 248 246 234 238 + Hpy188I TCNGA 236 235 . . 225 236 - BdaI TGANNNNNNTCA 248 246 214 212 240 245 + Cac8I GCNNGC 242 242 . . 240 245 + BmtI GCTAGC 244 240 . . 240 245 + NheI GCTAGC 240 244 . . 237 241 - SfaNI GCATC 227 231 . . 241 244 + MaeI CTAG 241 243 . . 243 246 + SetI ASST 246 242 . . 243 246 + CviJI RGCY 244 244 . . 243 246 + AluI AGCT 244 244 . . 251 254 + TspEI AATT 250 254 . . 256 259 + MseI TTAA 256 258 . . 263 267 + Hpy188I TCNGA 265 264 . . 281 284 + SetI ASST 284 280 . . 294 304 + Hin4I GAYNNNNNVTC 285 280 317 312 300 305 + XhoII RGATCY 300 304 . . 300 304 + BinI* GGATC 308 309 . . 301 304 + DpnI GATC 302 302 . . 301 304 + BstKTI GATC 303 301 . . 301 304 + MboI GATC 300 304 . . 294 304 - Hin4I GAYNNNNNVTC 317 312 285 280 304 307 + MaeI CTAG 304 306 . . 320 323 + TspEI AATT 319 323 . . 355 358 + FatI CATG 354 358 . . 355 358 + NlaIII CATG 358 354 . . 355 358 + CviAII CATG 355 357 . . 356 360 + TspDTI ATGAA 371 369 . . 361 370 + MslI CAYNNNNRTG 365 365 . . 367 371 - SfaNI GCATC 357 361 . . 371 376 + SfeI* CTRYAG 371 375 . . 376 380 + BslFI GGGAC 390 394 . . 392 395 + TaqI TCGA 392 394 . . 397 400 + TaiI ACGT 400 396 . . 397 400 + SetI ASST 400 396 . . 397 400 + MaeII ACGT 397 399 . . 399 404 + Hpy166II GTNNAC 401 401 . . 399 404 + HpaI GTTAAC 401 401 . . 399 404 + HindII GTYRAC 401 401 . . 400 403 + MseI TTAA 400 402 . . 419 423 + TfiI GAWTC 419 422 . . 419 423 + HinfI GANTC 419 422 . . 410 423 - AjuI GAANNNNNNNTTGG 435 430 403 398 427 433 + SauI* CCTNAGG 428 431 . . 428 432 + DdeI CTNAG 428 431 . . 464 467 + TaqI TCGA 464 466 . . 464 469 + Hpy178III* TCNNGA 465 467 . . 484 487 + MseI TTAA 484 486 . . 490 493 + TspEI AATT 489 493 . . 493 498 + AhaIII* TTTAAA 495 495 . . 494 497 + MseI TTAA 494 496 . . 499 504 + ApoI RAATTY 499 503 . . 500 503 + TspEI AATT 499 503 . . 502 506 - TspDTI ATGAA 492 490 . . 506 511 + Hpy178III* TCNNGA 507 509 . . 518 521 + TspEI AATT 517 521 . . 541 546 + EcoRV GATATC 543 543 . . 553 558 + ClaI ATCGAT 554 556 . . 554 557 + TaqI TCGA 554 556 . . 556 559 + DpnI GATC 557 557 . . 556 559 + BstKTI GATC 558 556 . . 556 559 + MboI GATC 555 559 . . 559 562 + MnlI CCTC 569 568 . . 556 560 - BinI* GGATC 550 551 . . 561 566 + Hpy178III* TCNNGA 562 564 . . 560 565 - BsiI* CACGAG 560 564 . . 569 573 + TspDTI ATGAA 584 582 . . 580 583 + CviRI* TGCA 581 581 . . 595 599 - Hin4II* CCTTC 589 588 . . 599 603 + HinfI GANTC 599 602 . . 599 603 - PleI GAGTC 593 594 . . 599 603 - MlyI GAGTC 593 593 . . 604 608 + HphI GGTGA 616 615 . . 616 621 + EcoRV GATATC 618 618 . . 620 625 + Hpy178III* TCNNGA 621 623 . . 620 625 + BspHI TCATGA 620 624 . . 621 624 + FatI CATG 620 624 . . 621 624 + NlaIII CATG 624 620 . . 621 624 + CviAII CATG 621 623 . . 622 626 + TspDTI ATGAA 637 635 . . 655 660 + ClaI ATCGAT 656 658 . . 656 659 + TaqI TCGA 656 658 . . 658 662 - BccI CCATC 652 653 . . 663 668 + AhaIII* TTTAAA 665 665 . . 664 667 + MseI TTAA 664 666 . . 683 688 + Hpy166II GTNNAC 685 685 . . 688 691 + FatI CATG 687 691 . . 688 691 + NlaIII CATG 691 687 . . 688 691 + CviAII CATG 688 690 . . 701 705 + Hpy188I TCNGA 703 702 . . 705 708 + TspEI AATT 704 708 . . 711 714 + CviJI RGCY 712 712 . . 713 717 + BssKI CCNGG 712 717 . . 713 717 + BseBI CCWGG 714 715 . . 713 718 + SecI* CCNNGG 713 717 . . 713 717 + ScrFI CCNGG 714 715 . . 713 717 + EcoRII CCWGG 712 717 . . 722 725 + SetI ASST 725 721 . . 739 744 + BspLU11I* ACATGT 739 743 . . 739 744 + NspI RCATGY 743 739 . . 739 744 + AflIII ACRYGT 739 743 . . 740 743 + FatI CATG 739 743 . . 740 743 + NlaIII CATG 743 739 . . 740 743 + CviAII CATG 740 742 . . 747 752 + ApoI RAATTY 747 751 . . 748 751 + TspEI AATT 747 751 . . 752 755 + AciI CCGC 752 754 . . 760 763 + MseI TTAA 760 762 . . 763 767 + TspDTI ATGAA 778 776 . . 784 787 + Csp6I GTAC 784 786 . . 784 787 + RsaI GTAC 785 785 . . 815 819 + BlsI GCNGC 817 816 . . 815 819 + BisI GCNGC 816 817 . . 815 819 + TseI GCWGC 815 818 . . 815 819 - BbvI GCAGC 802 806 . . 845 848 + SetI ASST 848 844 . . 855 858 + SetI ASST 858 854 . . 855 858 + CviJI RGCY 856 856 . . 855 858 + AluI AGCT 856 856 . . 861 864 + FatI CATG 860 864 . . 861 864 + NlaIII CATG 864 860 . . 861 864 + CviAII CATG 861 863 . . 865 870 + NlaIV GGNNCC 867 867 . . 868 871 + SetI ASST 871 867 . . 874 877 + FatI CATG 873 877 . . 874 877 + NlaIII CATG 877 873 . . 874 877 + CviAII CATG 874 876 . . 875 879 + TspDTI ATGAA 890 888 . . 877 881 + TfiI GAWTC 877 880 . . 877 881 + HinfI GANTC 877 880 . . 882 887 + SmlI CTYRAG 882 886 . . 882 887 + AflII CTTAAG 882 886 . . 883 886 + MseI TTAA 883 885 . . 888 893 + ApoI RAATTY 888 892 . . 889 892 + TspEI AATT 888 892 . . 902 907 + AclI AACGTT 903 905 . . 903 906 + TaiI ACGT 906 902 . . 903 906 + SetI ASST 906 902 . . 903 906 + MaeII ACGT 903 905 . . 908 913 + TatI WGTACW 908 912 . . 908 913 + Bsp1407I TGTACA 908 912 . . 909 912 + Csp6I GTAC 909 911 . . 909 912 + RsaI GTAC 910 910 . . 926 929 + TaiI ACGT 929 925 . . 926 929 + SetI ASST 929 925 . . 926 929 + MaeII ACGT 926 928 . . 933 936 + TspEI AATT 932 936 . . 934 939 + VspI ATTAAT 935 937 . . 935 938 + MseI TTAA 935 937 . . 939 944 + Hpy178III* TCNNGA 940 942 . . 943 947 + MboII GAAGA 955 954 . . 943 952 + XmnI GAANNNNTTC 947 947 . . 946 951 + ApoI RAATTY 946 950 . . 947 950 + TspEI AATT 946 950 . . 961 965 + Tsp4CI* ACNGT 963 962 . . 973 977 + Tsp4CI* ACNGT 975 974 . . 978 981 + TspEI AATT 977 981 . . 987 992 + SduI GDGCHC 991 987 . . 987 992 + HgiJII* GRGCYC 991 987 . . 988 991 + CviJI RGCY 989 989 . . 992 997 + MfeI CAATTG 992 996 . . 993 996 + TspEI AATT 992 996 . . 996 999 + CviRI* TGCA 997 997 . . 1003 1006 + DpnI GATC 1004 1004 . . 1003 1006 + BstKTI GATC 1005 1003 . . 1003 1006 + MboI GATC 1002 1006 . . 1018 1021 + SetI ASST 1021 1017 . . 1018 1021 + CviJI RGCY 1019 1019 . . 1018 1021 + AluI AGCT 1019 1019 . . 1019 1023 + BlsI GCNGC 1021 1020 . . 1019 1023 + BisI GCNGC 1020 1021 . . 1019 1023 + TseI GCWGC 1019 1022 . . 1021 1024 + CviRI* TGCA 1022 1022 . . 1022 1027 + NspI RCATGY 1026 1022 . . 1022 1032 + MwoI GCNNNNNNNGC 1028 1025 . . 1022 1032 + BstAPI GCANNNNNTGC 1028 1025 . . 1023 1026 + FatI CATG 1022 1026 . . 1023 1026 + NlaIII CATG 1026 1022 . . 1023 1026 + CviAII CATG 1023 1025 . . 1019 1023 - BbvI GCAGC 1006 1010 . . 1030 1033 + CviRI* TGCA 1031 1031 . . 1045 1050 - BsrDI GCAATG 1044 1042 . . 1053 1056 + TspEI AATT 1052 1056 . . 1055 1058 + MseI TTAA 1055 1057 . . 1060 1063 + CviRI* TGCA 1061 1061 . . 1084 1088 + DdeI CTNAG 1084 1087 . . 1087 1092 + StuI AGGCCT 1089 1089 . . 1088 1091 + CviJI RGCY 1089 1089 . . 1088 1091 + HaeIII GGCC 1089 1089 . . 1093 1097 + HinfI GANTC 1093 1096 . . 1096 1099 + TaqI TCGA 1096 1098 . . 1093 1097 - PleI GAGTC 1087 1088 . . 1093 1097 - MlyI GAGTC 1087 1087 . . 1102 1105 + TspEI AATT 1101 1105 . . 1114 1117 + SetI ASST 1117 1113 . . 1128 1131 + SetI ASST 1131 1127 . . 1130 1135 + Hpy166II GTNNAC 1132 1132 . . 1130 1135 + SduI GDGCHC 1134 1130 . . 1130 1135 + BseSI GKGCMC 1134 1130 . . 1130 1135 + ApaLI GTGCAC 1130 1134 . . 1130 1135 + HgiAI* GWGCWC 1134 1130 . . 1131 1134 + CviRI* TGCA 1132 1132 . . 1137 1141 - BsmAI GTCTC 1131 1135 . . 1150 1155 + SmlI CTYRAG 1150 1154 . . 1150 1155 + AflII CTTAAG 1150 1154 . . 1151 1154 + MseI TTAA 1151 1153 . . 1158 1164 + TspRI CASTGNN 1164 1155 . . 1161 1164 + CviRI* TGCA 1162 1162 . . 1157 1161 - BsrI ACTGG 1157 1155 . . 1211 1216 + Hpy166II GTNNAC 1213 1213 . . 1212 1217 + TatI WGTACW 1212 1216 . . 1212 1217 + Bsp1407I TGTACA 1212 1216 . . 1213 1216 + Csp6I GTAC 1213 1215 . . 1213 1216 + RsaI GTAC 1214 1214 . . 1228 1232 + BsrI ACTGG 1233 1231 . . 1234 1237 + TspEI AATT 1233 1237 . . #--------------------------------------- # # # AciI: # [CCGC] # 57 490 695 # # AclI: # [AACGTT] # 339 903 # # AcyI: # [GRCGYC] # 100 1142 # # AflII: # [CTTAAG] # 92 268 882 # # AflIII: # [ACRYGT] # 503 739 # # AhaIII*: # [TTTAAA] # 20 170 174 301 577 # # AjuI: # [GAANNNNNNNTTGG] # 32 403 807 # # AluI: # [AGCT] # 163 223 244 612 # # ApaLI: # [GTGCAC] # 112 1130 # # ApoI: # [RAATTY] # 58 141 248 296 499 # # BbvI: # [GCAGC] # 204 236 802 # # BccI: # [CCATC] # 111 541 590 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 214 994 # # BfiI: # [ACTGGG] # 4 1238 # # BinI*: # [GGATC] # 242 308 692 # # BisI: # [GCNGC] # 204 222 816 # # BlsI: # [GCNGC] # 204 221 817 # # BmtI: # [GCTAGC] # 244 998 # # BseBI: # [CCWGG] # 528 714 # # BseGI: # [GGATG] # 24 1218 # # BseMII: # [CTCAG] # 209 1033 # # BseSI: # [GKGCMC] # 108 1134 # # BsiI*: # [CACGAG] # 560 682 # # BsiYI*: # [CCNNNNNNNGG] # 1 14 1227 # # BslFI: # [GGGAC] # 390 852 # # BsmAI: # [GTCTC] # 111 214 917 # # Bsp1407I: # [TGTACA] # 30 304 908 # # BspCNI: # [CTCAG] # 210 1032 # # BspHI: # [TCATGA] # 620 622 # # BspLU11I*: # [ACATGT] # 503 739 # # BsrDI: # [GCAATG] # 198 1044 # # BsrI: # [ACTGG] # 9 10 76 1147 # # BssKI: # [CCNGG] # 530 712 # # BstAPI: # [GCANNNNNTGC] # 214 1028 # # BstKTI: # [GATC] # 237 255 303 447 # # Cac8I: # [GCNNGC] # 242 1000 # # ClaI: # [ATCGAT] # 102 554 586 # # Csp6I: # [GTAC] # 29 125 304 784 # # CviAII: # [CATG] # 13 52 67 121 149 219 # 266 355 # # CviJI: # [RGCY] # 30 70 133 144 153 244 # 468 # # CviRI*: # [TGCA] # 9 25 30 30 71 80 # 416 581 # # DdeI: # [CTNAG] # 158 210 218 656 # # DpnI: # [GATC] # 238 255 302 447 # # EcoRII: # [CCWGG] # 530 712 # # EcoRV: # [GATATC] # 75 543 624 # # FatI: # [CATG] # 13 52 67 121 149 220 # 266 354 # # FnuDII*: # [CGCG] # 57 1185 # # FokI: # [GGATG] # 31 1211 # # GlaI: # [GCGC] # 56 1186 # # HaeIII: # [GGCC] # 153 1089 # # HgaI: # [GACGC] # 108 1134 # # HgiAI*: # [GWGCWC] # 108 1134 # # HgiJII*: # [GRGCYC] # 251 991 # # HhaI: # [GCGC] # 57 1185 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 285 925 # # Hin4II*: # [CCTTC] # 589 653 # # Hin6I: # [GCGC] # 55 1187 # # HindII: # [GTYRAC] # 401 841 # # HinfI: # [GANTC] # 60 149 180 216 278 359 # # HpaI: # [GTTAAC] # 401 841 # # HphI: # [GGTGA] # 153 463 626 # # Hpy166II: # [GTNNAC] # 29 81 284 401 447 # # Hpy178III*: # [TCNNGA] # 42 55 59 302 319 465 # # Hpy188I: # [TCNGA] # 17 29 94 125 438 539 # # Ksp632I*: # [CTCTTC] # 197 1045 # # MaeI: # [CTAG] # 63 241 938 # # MaeII: # [ACGT] # 23 316 397 506 # # MboI: # [GATC] # 240 255 300 447 # # MboII: # [GAAGA] # 214 287 741 # # MfeI: # [CAATTG] # 250 992 # # MlyI: # [GAGTC] # 155 494 593 # # MnlI: # [CCTC] # 8 190 371 673 # # MseI: # [TTAA] # 10 10 16 20 35 52 # 63 84 91 96 96 112 # 120 123 144 170 # # MslI: # [CAYNNNNRTG] # 365 877 # # MwoI: # [GCNNNNNNNGC] # 214 1028 # # NheI: # [GCTAGC] # 240 1002 # # NlaIII: # [CATG] # 13 52 67 121 149 216 # 266 358 # # NlaIV: # [GGNNCC] # 375 867 # # NspI: # [RCATGY] # 216 283 743 # # PleI: # [GAGTC] # 155 494 593 # # RsaI: # [GTAC] # 28 125 304 785 # # SauI*: # [CCTNAGG] # 428 814 # # ScrFI: # [CCNGG] # 528 714 # # SduI: # [GDGCHC] # 108 143 991 # # SecI*: # [CCNNGG] # 529 713 # # SetI: # [ASST] # 10 13 14 23 29 35 # 38 74 92 96 111 116 # 123 143 325 # # SfaNI: # [GCATC] # 130 227 885 # # SfeI*: # [CTRYAG] # 371 871 # # SmlI: # [CTYRAG] # 92 268 882 # # StuI: # [AGGCCT] # 153 1089 # # TaiI: # [ACGT] # 23 313 400 506 # # TaqI: # [TCGA] # 67 72 90 102 146 325 # 440 # # TatI: # [WGTACW] # 30 304 908 # # TfiI: # [GAWTC] # 60 359 365 458 # # TseI: # [GCWGC] # 204 223 815 # # Tsp4CI*: # [ACNGT] # 12 90 267 873 # # TspDTI: # [ATGAA] # 35 53 92 112 114 121 # 141 222 352 # # TspEI: # [AATT] # 9 10 14 15 18 31 # 43 44 49 60 69 132 # 141 170 187 250 # # TspRI: # [CASTGNN] # 17 78 1147 # # VspI: # [ATTAAT] # 307 935 # # XhoII: # [RGATCY] # 300 942 # # XmnI: # [GAANNNNTTC] # 120 295 827 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1242 # Reported_sequences: 1 # Reported_hitcount: 258 #---------------------------------------