Restriction Fragments for YGRWdelta31



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGRWdelta31 on chromosome VII from coordinates 875951 to 876279.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25059.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 12 + TspDTI ATGAA 23 21 . . 19 22 + TspEI AATT 18 22 . . 67 71 + Tsp4CI* ACNGT 69 68 . . 79 83 - BccI CCATC 73 74 . . 99 102 - AciI CCGC 99 101 . . 94 104 - Hin4I GAYNNNNNVTC 117 112 85 80 115 118 - MnlI CCTC 108 107 . . 120 123 + SetI ASST 123 119 . . 120 123 + CviJI RGCY 121 121 . . 120 123 + AluI AGCT 121 121 . . 131 134 - MnlI CCTC 124 123 . . 150 154 + TspDTI ATGAA 165 163 . . 164 168 + TspGWI ACGGA 179 177 . . 171 174 - MnlI CCTC 164 163 . . 171 181 - Hin4I GAYNNNNNVTC 194 189 162 157 184 189 + SspI AATATT 186 186 . . 206 210 + TfiI GAWTC 206 209 . . 206 210 + HinfI GANTC 206 209 . . 210 220 + BsiYI* CCNNNNNNNGG 216 213 . . 220 224 + TfiI GAWTC 220 223 . . 217 220 - MnlI CCTC 210 209 . . 220 224 + HinfI GANTC 220 223 . . 225 228 + MaeI CTAG 225 227 . . 227 232 + XhoII RGATCY 227 231 . . 228 231 + DpnI GATC 229 229 . . 228 231 + BstKTI GATC 230 228 . . 228 231 + MboI GATC 227 231 . . 231 238 + PspXI VCTCGAGB 232 236 . . 231 234 + MnlI CCTC 241 240 . . 231 238 + AbsI CCTCGAGG 232 236 . . 232 237 + SmlI CTYRAG 232 236 . . 232 237 + XhoI CTCGAG 232 236 . . 232 237 + BmeT110I CYCGRG 233 235 . . 232 237 + AvaI CYCGRG 232 236 . . 228 232 - BinI* GGATC 222 223 . . 233 236 + TaqI TCGA 233 235 . . 235 240 + BseRI GAGGAG 250 248 . . 235 238 - MnlI CCTC 228 227 . . 246 249 + MaeI CTAG 246 248 . . 258 263 + Hpy166II GTNNAC 260 260 . . 258 263 + BssNAI GTATAC 260 260 . . 258 263 + AccI GTMKAC 259 261 . . 262 265 + SetI ASST 265 261 . . 266 271 + SspI AATATT 268 268 . . 274 277 + CviJI RGCY 275 275 . . 292 296 + TfiI GAWTC 292 295 . . 292 296 + HinfI GANTC 292 295 . . 302 305 + TspEI AATT 301 305 . . 307 312 + Hpy178III* TCNNGA 308 310 . . 309 312 + TaqI TCGA 309 311 . . 312 317 + ApoI RAATTY 312 316 . . 313 316 + TspEI AATT 312 316 . . 316 320 - HphI GGTGA 308 307 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 97 232 # # AccI: # [GTMKAC] # 70 259 # # AciI: # [CCGC] # 99 230 # # AluI: # [AGCT] # 121 208 # # ApoI: # [RAATTY] # 17 312 # # AvaI: # [CYCGRG] # 97 232 # # BccI: # [CCATC] # 73 256 # # BinI*: # [GGATC] # 107 222 # # BmeT110I: # [CYCGRG] # 96 233 # # BseRI: # [GAGGAG] # 79 250 # # BsiYI*: # [CCNNNNNNNGG] # 113 216 # # BssNAI: # [GTATAC] # 69 260 # # BstKTI: # [GATC] # 99 230 # # CviJI: # [RGCY] # 54 121 154 # # DpnI: # [GATC] # 100 229 # # Hin4I: # [GAYNNNNNVTC] # 32 32 45 85 135 # # HinfI: # [GANTC] # 14 37 72 206 # # HphI: # [GGTGA] # 21 308 # # Hpy166II: # [GTNNAC] # 69 260 # # Hpy178III*: # [TCNNGA] # 21 308 # # MaeI: # [CTAG] # 21 83 225 # # MboI: # [GATC] # 102 227 # # MnlI: # [CCTC] # 13 16 18 40 46 88 # 108 # # PspXI: # [VCTCGAGB] # 97 232 # # SetI: # [ASST] # 64 123 142 # # SmlI: # [CTYRAG] # 97 232 # # SspI: # [AATATT] # 61 82 186 # # TaqI: # [TCGA] # 20 76 233 # # TfiI: # [GAWTC] # 14 37 72 206 # # Tsp4CI*: # [ACNGT] # 69 260 # # TspDTI: # [ATGAA] # 23 142 164 # # TspEI: # [AATT] # 11 17 18 283 # # TspGWI: # [ACGGA] # 150 179 # # XhoI: # [CTCGAG] # 97 232 # # XhoII: # [RGATCY] # 102 227 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 329 # Reported_sequences: 1 # Reported_hitcount: 53 #---------------------------------------