Restriction Fragments for YGRWdelta26



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGRWdelta26 on chromosome VII from coordinates 793237 to 793559.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.10283.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + CviRI* TGCA 5 5 . . 12 16 + TfiI GAWTC 12 15 . . 12 16 + HinfI GANTC 12 15 . . 18 22 + MaeIII GTNAC 17 22 . . 22 26 + BccI CCATC 30 31 . . 24 29 + ClaI ATCGAT 25 27 . . 25 28 + TaqI TCGA 25 27 . . 26 31 + McrI* CGRYCG 29 27 . . 26 31 + PvuI CGATCG 29 27 . . 27 30 + DpnI GATC 28 28 . . 27 30 + BstKTI GATC 29 27 . . 27 30 + MboI GATC 26 30 . . 28 33 + ClaI ATCGAT 29 31 . . 29 32 + TaqI TCGA 29 31 . . 36 41 + EcoRI GAATTC 36 40 . . 36 41 + ApoI RAATTY 36 40 . . 37 40 + TspEI AATT 36 40 . . 39 43 - TspDTI ATGAA 29 27 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 50 55 + TatI WGTACW 50 54 . . 50 55 + ScaI AGTACT 52 52 . . 51 54 + Csp6I GTAC 51 53 . . 51 54 + RsaI GTAC 52 52 . . 57 60 + CviRI* TGCA 58 58 . . 64 70 + TspRI CASTGNN 70 61 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 74 78 + MboII GAAGA 86 85 . . 97 101 + Tsp4CI* ACNGT 99 98 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 110 113 + MseI TTAA 110 112 . . 126 131 + BsiI* CACGAG 126 130 . . 122 126 - BsmAI GTCTC 116 120 . . 129 132 - MnlI CCTC 122 121 . . 140 144 + TspDTI ATGAA 155 153 . . 165 169 + MboII GAAGA 177 176 . . 192 197 + EcoRV GATATC 194 194 . . 195 200 + ClaI ATCGAT 196 198 . . 196 199 + TaqI TCGA 196 198 . . 198 202 + TfiI GAWTC 198 201 . . 198 202 + HinfI GANTC 198 201 . . 202 212 + BsiYI* CCNNNNNNNGG 208 205 . . 203 213 + BsiYI* CCNNNNNNNGG 209 206 . . 212 217 + HaeII RGCGCY 216 212 . . 213 216 + Hin6I GCGC 213 215 . . 213 216 + GlaI GCGC 214 214 . . 213 216 + HhaI GCGC 215 213 . . 222 227 + Hpy178III* TCNNGA 223 225 . . 223 228 + Eco57MI CTGRAG 244 242 . . 223 228 + GsuI CTGGAG 244 242 . . 226 231 + BseRI GAGGAG 241 239 . . 226 229 - MnlI CCTC 219 218 . . 237 240 + MaeI CTAG 237 239 . . 268 279 + BdaI TGANNNNNNTCA 257 255 291 289 269 272 + DpnI GATC 270 270 . . 269 272 + BstKTI GATC 271 269 . . 269 278 + BsaBI GATNNNNATC 273 273 . . 269 272 + MboI GATC 268 272 . . 268 279 - BdaI TGANNNNNNTCA 291 289 257 255 280 284 + Tsp4CI* ACNGT 282 281 . . 281 287 + TspRI CASTGNN 287 278 . . 286 290 + TfiI GAWTC 286 289 . . 286 290 + HinfI GANTC 286 289 . . 301 304 + CviRI* TGCA 302 302 . . 304 307 + TspEI AATT 303 307 . . 310 314 - HphI GGTGA 302 301 . . #--------------------------------------- # # # ApoI: # [RAATTY] # 36 287 # # BccI: # [CCATC] # 30 293 # # BdaI: # [TGANNNNNNTCA] # 0 0 32 34 257 # # BsaBI: # [GATNNNNATC] # 50 273 # # BseRI: # [GAGGAG] # 82 241 # # BsiI*: # [CACGAG] # 126 197 # # BsiYI*: # [CCNNNNNNNGG] # 1 114 208 # # BsmAI: # [GTCTC] # 116 207 # # BstKTI: # [GATC] # 29 52 242 # # ClaI: # [ATCGAT] # 4 25 127 167 # # Csp6I: # [GTAC] # 51 272 # # CviRI*: # [TGCA] # 5 21 53 244 # # DpnI: # [GATC] # 28 53 242 # # Eco57MI: # [CTGRAG] # 79 244 # # EcoRI: # [GAATTC] # 36 287 # # EcoRV: # [GATATC] # 129 194 # # GlaI: # [GCGC] # 109 214 # # GsuI: # [CTGGAG] # 79 244 # # HaeII: # [RGCGCY] # 107 216 # # HhaI: # [GCGC] # 108 215 # # Hin4I: # [GAYNNNNNVTC] # 32 83 208 # # Hin6I: # [GCGC] # 110 213 # # HinfI: # [GANTC] # 12 37 88 186 # # HphI: # [GGTGA] # 21 302 # # Hpy178III*: # [TCNNGA] # 100 223 # # MaeI: # [CTAG] # 48 86 189 # # MaeIII: # [GTNAC] # 17 306 # # MboI: # [GATC] # 26 55 242 # # MboII: # [GAAGA] # 86 91 146 # # McrI*: # [CGRYCG] # 29 294 # # MnlI: # [CCTC] # 97 104 122 # # MseI: # [TTAA] # 110 213 # # PvuI: # [CGATCG] # 29 294 # # RsaI: # [GTAC] # 52 271 # # ScaI: # [AGTACT] # 52 271 # # SpeI: # [ACTAGT] # 47 276 # # TaqI: # [TCGA] # 4 25 127 167 # # TatI: # [WGTACW] # 50 273 # # TfiI: # [GAWTC] # 12 37 88 186 # # Tsp4CI*: # [ACNGT] # 32 41 67 183 # # TspDTI: # [ATGAA] # 29 126 168 # # TspEI: # [AATT] # 20 36 267 # # TspRI: # [CASTGNN] # 36 70 217 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 323 # Reported_sequences: 1 # Reported_hitcount: 66 #---------------------------------------