Restriction Fragments for YGRCdelta25



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The currently selected gene/sequence is :

YGRCdelta25 on chromosome VII from coordinates 779515 to 779225.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.1831.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 22 26 + BccI CCATC 30 31 . . 26 30 + BccI CCATC 34 35 . . 23 27 - FokI GGATG 9 13 . . 23 27 - BseGI GGATG 22 20 . . 28 33 + ClaI ATCGAT 29 31 . . 29 32 + TaqI TCGA 29 31 . . 41 44 + FatI CATG 40 44 . . 41 44 + NlaIII CATG 44 40 . . 41 44 + CviAII CATG 41 43 . . 39 43 - TspDTI ATGAA 29 27 . . 44 48 + Tsp45I GTSAC 43 48 . . 44 48 + MaeIII GTNAC 43 48 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 55 60 + VspI ATTAAT 56 58 . . 56 59 + MseI TTAA 56 58 . . 58 61 + TspEI AATT 57 61 . . 81 84 - MnlI CCTC 74 73 . . 101 105 + Tsp4CI* ACNGT 103 102 . . 110 113 + SetI ASST 113 109 . . 114 117 + MseI TTAA 114 116 . . 121 126 + HindIII AAGCTT 121 125 . . 122 125 + SetI ASST 125 121 . . 122 125 + CviJI RGCY 123 123 . . 122 125 + AluI AGCT 123 123 . . 125 128 + MseI TTAA 125 127 . . 135 139 + BinI* GGATC 143 144 . . 133 136 - MnlI CCTC 126 125 . . 136 139 + DpnI GATC 137 137 . . 136 139 + BstKTI GATC 138 136 . . 136 139 + MboI GATC 135 139 . . 137 142 + ClaI ATCGAT 138 140 . . 138 141 + TaqI TCGA 138 140 . . 141 145 + TspDTI ATGAA 156 154 . . 196 199 + SetI ASST 199 195 . . 209 213 + TfiI GAWTC 209 212 . . 209 213 + HinfI GANTC 209 212 . . 220 224 + TfiI GAWTC 220 223 . . 220 224 + HinfI GANTC 220 223 . . 233 236 + CviRI* TGCA 234 234 . . 237 241 + MboII GAAGA 249 248 . . 274 277 + CviJI RGCY 275 275 . . 279 283 + MaeIII GTNAC 278 283 . . 287 290 + TspEI AATT 286 290 . . #--------------------------------------- # # # AluI: # [AGCT] # 123 168 # # BccI: # [CCATC] # 4 30 257 # # BinI*: # [GGATC] # 143 148 # # BseGI: # [GGATG] # 22 269 # # BstKTI: # [GATC] # 138 153 # # ClaI: # [ATCGAT] # 29 109 153 # # CviAII: # [CATG] # 41 250 # # CviJI: # [RGCY] # 16 123 152 # # CviRI*: # [TGCA] # 57 234 # # DpnI: # [GATC] # 137 154 # # FatI: # [CATG] # 40 251 # # FokI: # [GGATG] # 9 282 # # HindIII: # [AAGCTT] # 121 170 # # HinfI: # [GANTC] # 11 71 209 # # MaeI: # [CTAG] # 48 243 # # MaeIII: # [GTNAC] # 13 43 235 # # MboI: # [GATC] # 135 156 # # MboII: # [GAAGA] # 42 249 # # MnlI: # [CCTC] # 52 74 165 # # MseI: # [TTAA] # 11 56 58 166 # # NlaIII: # [CATG] # 44 247 # # SetI: # [ASST] # 12 74 92 113 # # SpeI: # [ACTAGT] # 47 244 # # TaqI: # [TCGA] # 29 109 153 # # TfiI: # [GAWTC] # 11 71 209 # # Tsp45I: # [GTSAC] # 43 248 # # Tsp4CI*: # [ACNGT] # 103 188 # # TspDTI: # [ATGAA] # 29 127 135 # # TspEI: # [AATT] # 5 57 229 # # VspI: # [ATTAAT] # 56 235 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 291 # Reported_sequences: 1 # Reported_hitcount: 44 #---------------------------------------