Restriction Fragments for YGRCdelta20



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGRCdelta20 on chromosome VII from coordinates 713384 to 713084.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.19137.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 21 24 + SetI ASST 24 20 . . 22 25 + MnlI CCTC 32 31 . . 24 28 - TspGWI ACGGA 14 12 . . 36 40 - MboII GAAGA 28 27 . . 35 40 - BbvII* GAAGAC 28 32 . . 38 42 - TspDTI ATGAA 28 26 . . 46 51 + SpeI ACTAGT 46 50 . . 47 50 + MaeI CTAG 47 49 . . 87 90 + TspEI AATT 86 90 . . 93 97 + TfiI GAWTC 93 96 . . 93 97 + HinfI GANTC 93 96 . . 91 101 - Hin4I GAYNNNNNVTC 114 109 82 77 105 110 + ApoI RAATTY 105 109 . . 106 109 + TspEI AATT 105 109 . . 117 120 + CviJI RGCY 118 118 . . 119 122 + HpaII CCGG 119 121 . . 134 137 + CviRI* TGCA 135 135 . . 140 143 + CviRI* TGCA 141 141 . . 152 156 + TspDTI ATGAA 167 165 . . 198 201 - AciI CCGC 198 200 . . 203 208 + EcoRV GATATC 205 205 . . 209 214 + NlaIV GGNNCC 211 211 . . 222 227 + EcoRI GAATTC 222 226 . . 222 227 + ApoI RAATTY 222 226 . . 223 226 + TspEI AATT 222 226 . . 235 239 + TspDTI ATGAA 250 248 . . 237 241 - Hin4II* CCTTC 231 230 . . 243 248 + ApoI RAATTY 243 247 . . 244 247 + TspEI AATT 243 247 . . 252 257 + Hpy166II GTNNAC 254 254 . . 252 257 + BssNAI GTATAC 254 254 . . 252 257 + AccI GTMKAC 253 255 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 48 253 # # AciI: # [CCGC] # 103 198 # # ApoI: # [RAATTY] # 21 58 105 117 # # BbvII*: # [GAAGAC] # 28 273 # # BssNAI: # [GTATAC] # 47 254 # # CviJI: # [RGCY] # 118 183 # # CviRI*: # [TGCA] # 6 135 160 # # EcoRI: # [GAATTC] # 79 222 # # EcoRV: # [GATATC] # 96 205 # # Hin4I: # [GAYNNNNNVTC] # 32 82 187 # # Hin4II*: # [CCTTC] # 70 231 # # HinfI: # [GANTC] # 93 208 # # HpaII: # [CCGG] # 119 182 # # Hpy166II: # [GTNNAC] # 47 254 # # MaeI: # [CTAG] # 47 254 # # MboII: # [GAAGA] # 28 273 # # MnlI: # [CCTC] # 32 269 # # NlaIV: # [GGNNCC] # 90 211 # # SetI: # [ASST] # 24 277 # # SpeI: # [ACTAGT] # 46 255 # # TfiI: # [GAWTC] # 93 208 # # TspDTI: # [ATGAA] # 28 51 83 139 # # TspEI: # [AATT] # 19 21 58 86 117 # # TspGWI: # [ACGGA] # 14 287 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 301 # Reported_sequences: 1 # Reported_hitcount: 32 #---------------------------------------