Restriction Fragments for PFK1/YGR240C



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The currently selected gene/sequence is :

PFK1/YGR240C on chromosome VII from coordinates 973734 to 970771.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.27746.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 8 13 + SmlI CTYRAG 8 12 . . 9 14 + Hpy178III* TCNNGA 10 12 . . 13 17 + TfiI GAWTC 13 16 . . 13 17 + HinfI GANTC 13 16 . . 17 20 + FatI CATG 16 20 . . 17 20 + NlaIII CATG 20 16 . . 17 20 + CviAII CATG 17 19 . . 15 19 - TspDTI ATGAA 5 3 . . 23 27 + Tsp4CI* ACNGT 25 24 . . 30 33 + CviRI* TGCA 31 31 . . 35 39 + Hpy188I TCNGA 37 36 . . 31 36 - BsmI GAATGC 31 29 . . 37 42 + XhoII RGATCY 37 41 . . 37 42 + BglII AGATCT 37 41 . . 38 48 + Hin4I GAYNNNNNVTC 29 24 61 56 38 41 + DpnI GATC 39 39 . . 38 41 + BstKTI GATC 40 38 . . 38 41 + MboI GATC 37 41 . . 38 48 - Hin4I GAYNNNNNVTC 61 56 29 24 56 60 + TspDTI ATGAA 71 69 . . 59 64 + HindIII AAGCTT 59 63 . . 60 63 + SetI ASST 63 59 . . 60 63 + CviJI RGCY 61 61 . . 60 63 + AluI AGCT 61 61 . . 68 73 + Hpy178III* TCNNGA 69 71 . . 72 76 + MboII GAAGA 84 83 . . 72 77 + BbvII* GAAGAC 79 83 . . 97 100 + MaeI CTAG 97 99 . . 105 108 + CviRI* TGCA 106 106 . . 109 113 + Tsp4CI* ACNGT 111 110 . . 113 124 + BdaI TGANNNNNNTCA 102 100 136 134 114 123 + XmnI GAANNNNTTC 118 118 . . 113 124 - BdaI TGANNNNNNTCA 136 134 102 100 127 132 + ApoI RAATTY 127 131 . . 128 131 + TspEI AATT 127 131 . . 136 139 + FatI CATG 135 139 . . 136 139 + NlaIII CATG 139 135 . . 136 139 + CviAII CATG 136 138 . . 139 143 + HphI GGTGA 151 150 . . 148 151 + SetI ASST 151 147 . . 148 151 + CviJI RGCY 149 149 . . 148 151 + AluI AGCT 149 149 . . 157 161 - MboII GAAGA 149 148 . . 158 163 - Eco57MI CTGRAG 143 141 . . 163 167 + BslFI GGGAC 177 181 . . 158 163 - Eco57I CTGAAG 143 141 . . 175 179 + TfiI GAWTC 175 178 . . 175 179 + HinfI GANTC 175 178 . . 180 185 + SmlI CTYRAG 180 184 . . 180 185 + Bce83I* CTTGAG 201 199 . . 193 198 - TsoI TARCCA 183 181 . . 199 203 + TfiI GAWTC 199 202 . . 199 203 + HinfI GANTC 199 202 . . 218 221 + SetI ASST 221 217 . . 217 220 - MnlI CCTC 210 209 . . 223 227 - SfaNI GCATC 213 217 . . 233 238 + NlaIV GGNNCC 235 235 . . 239 244 + Hpy166II GTNNAC 241 241 . . 239 244 + BssNAI GTATAC 241 241 . . 239 244 + AccI GTMKAC 240 242 . . 236 240 - TspGWI ACGGA 226 224 . . 255 258 + SetI ASST 258 254 . . 255 258 + CviJI RGCY 256 256 . . 255 258 + AluI AGCT 256 256 . . 267 270 + CviJI RGCY 268 268 . . 279 282 + SetI ASST 282 278 . . 281 286 + SduI GDGCHC 285 281 . . 281 286 + HgiAI* GWGCWC 285 281 . . 289 292 + MseI TTAA 289 291 . . 294 298 + TfiI GAWTC 294 297 . . 294 298 + HinfI GANTC 294 297 . . 306 316 + Hin4I GAYNNNNNVTC 297 292 329 324 319 324 + ClaI ATCGAT 320 322 . . 320 323 + TaqI TCGA 320 322 . . 323 327 + TspDTI ATGAA 338 336 . . 332 335 + TaqI TCGA 332 334 . . 347 350 + AciI CCGC 347 349 . . 389 392 + TaqI TCGA 389 391 . . 389 394 + Hpy178III* TCNNGA 390 392 . . 394 399 + ApoI RAATTY 394 398 . . 395 398 + TspEI AATT 394 398 . . 408 411 + CviJI RGCY 409 409 . . 416 419 + TspEI AATT 415 419 . . 421 425 + Hpy188I TCNGA 423 422 . . 427 430 + SetI ASST 430 426 . . 429 433 + DdeI CTNAG 429 432 . . 424 434 - BaeI ACNNNNGTAYC 449 444 416 411 444 449 + XhoII RGATCY 444 448 . . 445 448 + DpnI GATC 446 446 . . 445 448 + BstKTI GATC 447 445 . . 445 448 + MboI GATC 444 448 . . 445 449 - BinI* GGATC 439 440 . . 466 469 + SetI ASST 469 465 . . 467 471 + Hin4II* CCTTC 477 476 . . 464 468 - HphI GGTGA 456 455 . . 471 475 + DdeI CTNAG 471 474 . . 471 475 + BspCNI CTCAG 484 482 . . 471 475 + BseMII CTCAG 485 483 . . 475 478 + CviJI RGCY 476 476 . . 476 482 + EspI* GCTNAGC 477 480 . . 477 481 + DdeI CTNAG 477 480 . . 480 483 + SetI ASST 483 479 . . 480 483 + CviJI RGCY 481 481 . . 480 483 + AluI AGCT 481 481 . . 486 489 + CviRI* TGCA 487 487 . . 493 497 + TfiI GAWTC 493 496 . . 493 497 + HinfI GANTC 493 496 . . 497 500 + AciI CCGC 497 499 . . 497 502 + BsrBI CCGCTC 499 499 . . 501 506 + MmeI TCCRAC 526 524 . . 514 518 - SfaNI GCATC 504 508 . . 522 526 + DdeI CTNAG 522 525 . . 509 522 - AjuI GAANNNNNNNTTGG 534 529 502 497 528 531 + SetI ASST 531 527 . . 528 531 + CviJI RGCY 529 529 . . 528 531 + AluI AGCT 529 529 . . 532 535 + SetI ASST 535 531 . . 533 536 + MnlI CCTC 543 542 . . 535 539 + Hpy188I TCNGA 537 536 . . 538 542 + MboII GAAGA 550 549 . . 538 543 + BbvII* GAAGAC 545 549 . . 553 558 + XbaI TCTAGA 553 557 . . 553 558 + Hpy178III* TCNNGA 554 556 . . 554 557 + MaeI CTAG 554 556 . . 558 561 + SetI ASST 561 557 . . 558 561 + CviJI RGCY 559 559 . . 558 561 + AluI AGCT 559 559 . . 565 569 + Hpy188I TCNGA 567 566 . . 562 566 - MboII GAAGA 554 553 . . 568 572 - SfaNI GCATC 558 562 . . 574 578 + Hpy188I TCNGA 576 575 . . 583 587 + DdeI CTNAG 583 586 . . 597 602 + SduI GDGCHC 601 597 . . 597 602 + HgiJII* GRGCYC 601 597 . . 598 601 + CviJI RGCY 599 599 . . 599 605 + SapI GCTCTTC 606 609 . . 600 605 + Ksp632I* CTCTTC 606 609 . . 601 605 - MboII GAAGA 593 592 . . 612 616 + MboII GAAGA 624 623 . . 615 619 + MboII GAAGA 627 626 . . 618 622 + MboII GAAGA 630 629 . . 629 634 + Hpy178III* TCNNGA 630 632 . . 629 634 + BspHI TCATGA 629 633 . . 630 633 + FatI CATG 629 633 . . 630 633 + NlaIII CATG 633 629 . . 630 633 + CviAII CATG 630 632 . . 643 647 + HphI GGTGA 655 654 . . 646 650 + TfiI GAWTC 646 649 . . 646 650 + HinfI GANTC 646 649 . . 652 656 + BssKI CCNGG 651 656 . . 652 656 + BseBI CCWGG 653 654 . . 652 656 + ScrFI CCNGG 653 654 . . 652 656 + EcoRII CCWGG 651 656 . . 654 657 + SetI ASST 657 653 . . 650 655 - Eco57MI CTGRAG 635 633 . . 650 655 - GsuI CTGGAG 635 633 . . 658 662 + TspDTI ATGAA 673 671 . . 660 665 + BsmI GAATGC 666 664 . . 664 668 + TauI GCSGC 667 664 . . 664 668 + BlsI GCNGC 666 665 . . 664 668 + BisI GCNGC 665 666 . . 665 670 + NspBII* CMGCKG 667 667 . . 665 668 + AciI CCGC 665 667 . . 667 677 + MwoI GCNNNNNNNGC 673 670 . . 676 680 - BceAI ACGGC 661 663 . . 686 689 + Csp6I GTAC 686 688 . . 686 689 + RsaI GTAC 687 687 . . 690 693 + SetI ASST 693 689 . . 692 697 + Hpy166II GTNNAC 694 694 . . 692 697 + BssNAI GTATAC 694 694 . . 692 697 + AccI GTMKAC 693 695 . . 703 706 + CviJI RGCY 704 704 . . 721 726 + Hpy166II GTNNAC 723 723 . . 729 732 + SetI ASST 732 728 . . 731 735 + MaeIII GTNAC 730 735 . . 727 731 - Hin4II* CCTTC 721 720 . . 738 741 + SetI ASST 741 737 . . 736 740 - Hin4II* CCTTC 730 729 . . 740 745 + Hpy166II GTNNAC 742 742 . . 745 749 + DdeI CTNAG 745 748 . . 749 752 - MnlI CCTC 742 741 . . 752 755 - AciI CCGC 752 754 . . 758 763 + SspI AATATT 760 760 . . 762 767 + AhaIII* TTTAAA 764 764 . . 763 766 + MseI TTAA 763 765 . . 774 777 + CviJI RGCY 775 775 . . 781 785 + MboII GAAGA 793 792 . . 788 792 + Hpy188I TCNGA 790 789 . . 792 795 + SetI ASST 795 791 . . 791 794 - MnlI CCTC 784 783 . . 799 802 + MseI TTAA 799 801 . . 796 801 - TsoI TARCCA 786 784 . . 807 810 + SetI ASST 810 806 . . 805 809 - Hin4II* CCTTC 799 798 . . 812 815 + Csp6I GTAC 812 814 . . 812 815 + RsaI GTAC 813 813 . . 824 827 + Csp6I GTAC 824 826 . . 824 827 + RsaI GTAC 825 825 . . 824 836 - PsrI GAACNNNNNNTAC 848 843 816 811 841 846 + EcoRI GAATTC 841 845 . . 841 846 + ApoI RAATTY 841 845 . . 842 845 + TspEI AATT 841 845 . . 845 849 + Hpy188I TCNGA 847 846 . . 856 859 - MnlI CCTC 849 848 . . 863 868 + Hpy166II GTNNAC 865 865 . . 863 868 + AccI GTMKAC 864 866 . . 870 873 + SetI ASST 873 869 . . 870 873 + CviJI RGCY 871 871 . . 870 873 + AluI AGCT 871 871 . . 871 875 + BlsI GCNGC 873 872 . . 871 875 + BisI GCNGC 872 873 . . 871 875 + TseI GCWGC 871 874 . . 872 877 + PstI CTGCAG 876 872 . . 872 877 + SfeI* CTRYAG 872 876 . . 873 876 + CviRI* TGCA 874 874 . . 874 879 + Cac8I GCNNGC 876 876 . . 871 875 - BbvI GCAGC 858 862 . . 880 883 + TspEI AATT 879 883 . . 883 886 + MseI TTAA 883 885 . . 885 888 + TspEI AATT 884 888 . . 894 897 + SetI ASST 897 893 . . 901 905 + HgaI GACGC 910 915 . . 922 926 + HphI GGTGA 934 933 . . 925 929 - BccI CCATC 919 920 . . 934 937 + MseI TTAA 934 936 . . 937 942 + Cfr10I RCCGGY 937 941 . . 937 942 + BetI* WCCGGW 937 941 . . 937 942 + AgeI ACCGGT 937 941 . . 938 941 + HpaII CCGG 938 940 . . 946 949 + DpnI GATC 947 947 . . 946 949 + BstKTI GATC 948 946 . . 946 949 + MboI GATC 945 949 . . 953 957 + Hpy188I TCNGA 955 954 . . 964 969 + BalI TGGCCA 966 966 . . 964 969 + CfrI YGGCCR 964 968 . . 965 968 + CviJI RGCY 966 966 . . 965 968 + HaeIII GGCC 966 966 . . 967 971 + BccI CCATC 975 976 . . 980 984 + TspDTI ATGAA 995 993 . . 983 986 + TspEI AATT 982 986 . . 990 993 + CviRI* TGCA 991 991 . . 996 999 + SetI ASST 999 995 . . 994 998 - Hin4II* CCTTC 988 987 . . 1001 1005 + TfiI GAWTC 1001 1004 . . 1001 1005 + HinfI GANTC 1001 1004 . . 1012 1016 + MboII GAAGA 1024 1023 . . 1032 1037 + ApoI RAATTY 1032 1036 . . 1033 1036 + TspEI AATT 1032 1036 . . 1057 1062 + NlaIV GGNNCC 1059 1059 . . 1061 1065 + BccI CCATC 1069 1070 . . 1063 1068 + ClaI ATCGAT 1064 1066 . . 1064 1067 + TaqI TCGA 1064 1066 . . 1082 1085 + Csp6I GTAC 1082 1084 . . 1082 1085 + RsaI GTAC 1083 1083 . . 1087 1091 + HinfI GANTC 1087 1090 . . 1087 1091 - PleI GAGTC 1081 1082 . . 1087 1091 - MlyI GAGTC 1081 1081 . . 1102 1112 + MwoI GCNNNNNNNGC 1108 1105 . . 1121 1125 + TfiI GAWTC 1121 1124 . . 1121 1125 + HinfI GANTC 1121 1124 . . 1135 1140 + Hpy166II GTNNAC 1137 1137 . . 1135 1140 + HindII GTYRAC 1137 1137 . . 1147 1151 - SfaNI GCATC 1137 1141 . . 1154 1157 + AciI CCGC 1154 1156 . . 1165 1174 + MslI CAYNNNNRTG 1169 1169 . . 1165 1174 + OliI CACNNNNGTG 1169 1169 . . 1196 1201 + Hpy166II GTNNAC 1198 1198 . . 1196 1201 + AccI GTMKAC 1197 1199 . . 1215 1218 + CviJI RGCY 1216 1216 . . 1215 1218 + HaeIII GGCC 1216 1216 . . 1216 1226 + MwoI GCNNNNNNNGC 1222 1219 . . 1216 1226 + BglI GCCNNNNNGGC 1222 1219 . . 1224 1227 + CviJI RGCY 1225 1225 . . 1225 1235 + MwoI GCNNNNNNNGC 1231 1228 . . 1221 1225 - BccI CCATC 1215 1216 . . 1234 1244 + MwoI GCNNNNNNNGC 1240 1237 . . 1237 1242 + Cfr10I RCCGGY 1237 1241 . . 1237 1242 + BetI* WCCGGW 1237 1241 . . 1237 1242 + AgeI ACCGGT 1237 1241 . . 1238 1241 + HpaII CCGG 1238 1240 . . 1240 1245 + NlaIV GGNNCC 1242 1242 . . 1240 1245 + HgiCI* GGYRCC 1240 1244 . . 1260 1265 + Hpy178III* TCNNGA 1261 1263 . . 1269 1272 + SetI ASST 1272 1268 . . 1269 1272 + CviJI RGCY 1270 1270 . . 1269 1272 + AluI AGCT 1270 1270 . . 1276 1279 + MnlI CCTC 1286 1285 . . 1280 1284 + TspGWI ACGGA 1295 1293 . . 1298 1301 + TspEI AATT 1297 1301 . . 1302 1306 - Hin4II* CCTTC 1296 1295 . . 1337 1341 + MboII GAAGA 1349 1348 . . 1350 1353 + TspEI AATT 1349 1353 . . 1360 1370 + MwoI GCNNNNNNNGC 1366 1363 . . 1361 1366 + Eco57MI CTGRAG 1382 1380 . . 1361 1366 + Eco57I CTGAAG 1382 1380 . . 1365 1368 + SetI ASST 1368 1364 . . 1363 1367 - Hin4II* CCTTC 1357 1356 . . 1377 1382 + BclI TGATCA 1377 1381 . . 1378 1381 + DpnI GATC 1379 1379 . . 1378 1381 + BstKTI GATC 1380 1378 . . 1378 1381 + MboI GATC 1377 1381 . . 1382 1385 + TspEI AATT 1381 1385 . . 1384 1387 + MseI TTAA 1384 1386 . . 1393 1397 + MaeIII GTNAC 1392 1397 . . 1405 1410 + ZraI GACGTC 1407 1407 . . 1405 1410 + AcyI GRCGYC 1406 1408 . . 1405 1410 + AatII GACGTC 1409 1405 . . 1406 1409 + TaiI ACGT 1409 1405 . . 1406 1409 + SetI ASST 1409 1405 . . 1406 1409 + MaeII ACGT 1406 1408 . . 1414 1418 - SfaNI GCATC 1404 1408 . . 1427 1430 + TspEI AATT 1426 1430 . . 1434 1439 + XbaI TCTAGA 1434 1438 . . 1434 1439 + Hpy178III* TCNNGA 1435 1437 . . 1435 1438 + MaeI CTAG 1435 1437 . . 1442 1447 + StyI CCWWGG 1442 1446 . . 1442 1447 + SecI* CCNNGG 1442 1446 . . 1445 1448 + SetI ASST 1448 1444 . . 1446 1452 + BstEII GGTNACC 1446 1451 . . 1447 1451 + MaeIII GTNAC 1446 1451 . . 1448 1453 + TsoI TARCCA 1464 1462 . . 1456 1459 + MaeI CTAG 1456 1458 . . 1458 1461 + SetI ASST 1461 1457 . . 1460 1464 + Tsp45I GTSAC 1459 1464 . . 1460 1464 + MaeIII GTNAC 1459 1464 . . 1463 1466 + TaiI ACGT 1466 1462 . . 1463 1466 + SetI ASST 1466 1462 . . 1463 1466 + MaeII ACGT 1463 1465 . . 1473 1476 + SetI ASST 1476 1472 . . 1472 1475 - MnlI CCTC 1465 1464 . . 1478 1481 + Csp6I GTAC 1478 1480 . . 1478 1481 + RsaI GTAC 1479 1479 . . 1481 1486 + PvuII CAGCTG 1483 1483 . . 1481 1486 + NspBII* CMGCKG 1483 1483 . . 1482 1485 + SetI ASST 1485 1481 . . 1482 1485 + CviJI RGCY 1483 1483 . . 1482 1485 + AluI AGCT 1483 1483 . . 1477 1487 - BaeI ACNNNNGTAYC 1502 1497 1469 1464 1491 1496 + Hpy178III* TCNNGA 1492 1494 . . 1491 1496 + BspHI TCATGA 1491 1495 . . 1492 1495 + FatI CATG 1491 1495 . . 1492 1495 + NlaIII CATG 1495 1491 . . 1492 1495 + CviAII CATG 1492 1494 . . 1499 1503 - BccI CCATC 1493 1494 . . 1501 1506 - TsoI TARCCA 1491 1489 . . 1506 1509 + SetI ASST 1509 1505 . . 1506 1509 + CviJI RGCY 1507 1507 . . 1506 1509 + AluI AGCT 1507 1507 . . 1518 1521 + SetI ASST 1521 1517 . . 1523 1526 + TaqI TCGA 1523 1525 . . 1528 1538 + MwoI GCNNNNNNNGC 1534 1531 . . 1525 1529 - SfaNI GCATC 1515 1519 . . 1532 1535 + MseI TTAA 1532 1534 . . 1536 1539 + CviJI RGCY 1537 1537 . . 1536 1539 + HaeIII GGCC 1537 1537 . . 1537 1541 - BceAI ACGGC 1522 1524 . . 1542 1547 + Hpy178III* TCNNGA 1543 1545 . . 1546 1551 + ApoI RAATTY 1546 1550 . . 1547 1550 + TspEI AATT 1546 1550 . . 1561 1571 + BsaXI ACNNNNNCTCC 1551 1548 1581 1578 1564 1568 + Hin4II* CCTTC 1574 1573 . . 1570 1580 + BsiYI* CCNNNNNNNGG 1576 1573 . . 1570 1580 + PflMI CCANNNNNTGG 1576 1573 . . 1572 1577 + VspI ATTAAT 1573 1575 . . 1573 1576 + MseI TTAA 1573 1575 . . 1575 1578 + TspEI AATT 1574 1578 . . 1599 1602 + TspEI AATT 1598 1602 . . 1604 1609 + BsmI GAATGC 1610 1608 . . 1618 1622 + TfiI GAWTC 1618 1621 . . 1618 1622 + HinfI GANTC 1618 1621 . . 1629 1634 + Hpy166II GTNNAC 1631 1631 . . 1629 1634 + HindII GTYRAC 1631 1631 . . 1647 1653 + TspRI CASTGNN 1653 1644 . . 1647 1652 - BtsI GCAGTG 1646 1644 . . 1646 1657 - CspCI CAANNNNNGTGG 1670 1668 1635 1633 1670 1673 + TaqI TCGA 1670 1672 . . 1680 1683 + TspEI AATT 1679 1683 . . 1687 1690 + MseI TTAA 1687 1689 . . 1691 1695 - BsmAI GTCTC 1685 1689 . . 1699 1704 + ApoI RAATTY 1699 1703 . . 1700 1703 + TspEI AATT 1699 1703 . . 1735 1739 + Tsp4CI* ACNGT 1737 1736 . . 1739 1742 + MseI TTAA 1739 1741 . . 1750 1755 + NlaIV GGNNCC 1752 1752 . . 1747 1751 - BccI CCATC 1741 1742 . . 1753 1757 + Hpy188I TCNGA 1755 1754 . . 1757 1760 + TspEI AATT 1756 1760 . . 1766 1774 + AlwNI CAGNNNCTG 1771 1768 . . 1765 1769 - BsrI ACTGG 1765 1763 . . 1771 1775 + Hpy188I TCNGA 1773 1772 . . 1798 1807 + MslI CAYNNNNRTG 1802 1802 . . 1798 1801 + FatI CATG 1797 1801 . . 1798 1801 + NlaIII CATG 1801 1797 . . 1798 1801 + CviAII CATG 1798 1800 . . 1804 1809 + NlaIV GGNNCC 1806 1806 . . 1804 1809 + HgiCI* GGYRCC 1804 1808 . . 1805 1810 + SduI GDGCHC 1809 1805 . . 1805 1810 + BseSI GKGCMC 1809 1805 . . 1807 1817 + MwoI GCNNNNNNNGC 1813 1810 . . 1810 1814 + BccI CCATC 1818 1819 . . 1816 1820 + BlsI GCNGC 1818 1817 . . 1816 1820 + BisI GCNGC 1817 1818 . . 1816 1820 + TseI GCWGC 1816 1819 . . 1814 1819 - EcoP15I CAGCAG 1786 1788 . . 1819 1829 + MwoI GCNNNNNNNGC 1825 1822 . . 1816 1820 - BbvI GCAGC 1803 1807 . . 1828 1832 + BlsI GCNGC 1830 1829 . . 1828 1832 + BisI GCNGC 1829 1830 . . 1828 1832 + TseI GCWGC 1828 1831 . . 1831 1841 + MwoI GCNNNNNNNGC 1837 1834 . . 1828 1832 - BbvI GCAGC 1815 1819 . . 1840 1844 + TauI GCSGC 1843 1840 . . 1840 1844 + BlsI GCNGC 1842 1841 . . 1840 1844 + BisI GCNGC 1841 1842 . . 1841 1844 + AciI CCGC 1841 1843 . . 1853 1857 + Tsp4CI* ACNGT 1855 1854 . . 1860 1864 + BsmAI GTCTC 1865 1869 . . 1865 1869 + BceAI ACGGC 1881 1883 . . 1866 1871 + CfrI YGGCCR 1866 1870 . . 1867 1870 + CviJI RGCY 1868 1868 . . 1867 1870 + HaeIII GGCC 1868 1868 . . 1886 1891 + Hpy178III* TCNNGA 1887 1889 . . 1886 1891 + BspHI TCATGA 1886 1890 . . 1887 1890 + FatI CATG 1886 1890 . . 1887 1890 + NlaIII CATG 1890 1886 . . 1887 1890 + CviAII CATG 1887 1889 . . 1888 1892 + TspDTI ATGAA 1903 1901 . . 1889 1900 + BdaI TGANNNNNNTCA 1878 1876 1912 1910 1890 1899 + XmnI GAANNNNTTC 1894 1894 . . 1899 1905 + TspRI CASTGNN 1905 1896 . . 1889 1900 - BdaI TGANNNNNNTCA 1912 1910 1878 1876 1908 1912 + TfiI GAWTC 1908 1911 . . 1908 1912 + HinfI GANTC 1908 1911 . . 1915 1920 + Cfr10I RCCGGY 1915 1919 . . 1915 1920 + BetI* WCCGGW 1915 1919 . . 1915 1920 + AgeI ACCGGT 1915 1919 . . 1916 1919 + HpaII CCGG 1916 1918 . . 1918 1922 + HphI GGTGA 1930 1929 . . 1926 1930 - Hin4II* CCTTC 1920 1919 . . 1937 1940 + FatI CATG 1936 1940 . . 1937 1940 + NlaIII CATG 1940 1936 . . 1937 1940 + CviAII CATG 1937 1939 . . 1949 1952 + TaqI TCGA 1949 1951 . . 1955 1959 + BsrI ACTGG 1960 1958 . . 1967 1972 - TaqII CACCCA 1957 1955 . . 1972 1977 + NlaIV GGNNCC 1974 1974 . . 1975 1979 + Hpy188I TCNGA 1977 1976 . . 1985 1988 + Csp6I GTAC 1985 1987 . . 1985 1988 + RsaI GTAC 1986 1986 . . 1993 1998 + XhoII RGATCY 1993 1997 . . 1993 1998 + BglII AGATCT 1993 1997 . . 1994 1997 + DpnI GATC 1995 1995 . . 1994 1997 + BstKTI GATC 1996 1994 . . 1994 1997 + MboI GATC 1993 1997 . . 2005 2009 + Hpy188I TCNGA 2007 2006 . . 2008 2012 + MboII GAAGA 2020 2019 . . 2003 2008 - Eco57MI CTGRAG 1988 1986 . . 2003 2008 - Eco57I CTGAAG 1988 1986 . . 2017 2022 + KpnI GGTACC 2021 2017 . . 2017 2022 + NlaIV GGNNCC 2019 2019 . . 2017 2022 + HgiCI* GGYRCC 2017 2021 . . 2017 2022 + Acc65I GGTACC 2017 2021 . . 2018 2021 + Csp6I GTAC 2018 2020 . . 2018 2021 + RsaI GTAC 2019 2019 . . 2022 2027 - BsrDI GCAATG 2021 2019 . . 2048 2051 + SetI ASST 2051 2047 . . 2048 2051 + CviJI RGCY 2049 2049 . . 2048 2051 + AluI AGCT 2049 2049 . . 2050 2053 + MaeI CTAG 2050 2052 . . 2054 2058 + Tsp4CI* ACNGT 2056 2055 . . 2082 2085 + SetI ASST 2085 2081 . . 2080 2084 - Hin4II* CCTTC 2074 2073 . . 2089 2092 + SetI ASST 2092 2088 . . 2089 2095 + PpuMI RGGWCCY 2090 2093 . . 2089 2095 + DraII RGGNCCY 2090 2093 . . 2090 2094 + BmgT120I GGNCC 2091 2092 . . 2090 2094 + AvaII GGWCC 2090 2093 . . 2090 2094 + AsuI* GGNCC 2090 2093 . . 2101 2106 + MfeI CAATTG 2101 2105 . . 2102 2105 + TspEI AATT 2101 2105 . . 2108 2112 + Tsp45I GTSAC 2107 2112 . . 2108 2112 + MaeIII GTNAC 2107 2112 . . 2111 2115 + Tsp4CI* ACNGT 2113 2112 . . 2125 2130 + TaqII CACCCA 2141 2139 . . 2135 2138 + MseI TTAA 2135 2137 . . 2139 2148 + MslI CAYNNNNRTG 2143 2143 . . 2154 2158 + TfiI GAWTC 2154 2157 . . 2154 2158 + HinfI GANTC 2154 2157 . . 2159 2167 + AlwNI CAGNNNCTG 2164 2161 . . 2160 2163 + CviJI RGCY 2161 2161 . . 2163 2169 + TspRI CASTGNN 2169 2160 . . 2164 2168 + Tsp4CI* ACNGT 2166 2165 . . 2176 2181 + AclI AACGTT 2177 2179 . . 2177 2180 + TaiI ACGT 2180 2176 . . 2177 2180 + SetI ASST 2180 2176 . . 2177 2180 + MaeII ACGT 2177 2179 . . 2182 2186 + BssKI CCNGG 2181 2186 . . 2182 2186 + BseBI CCWGG 2183 2184 . . 2182 2186 + ScrFI CCNGG 2183 2184 . . 2182 2186 + EcoRII CCWGG 2181 2186 . . 2183 2191 + AlwNI CAGNNNCTG 2188 2185 . . 2184 2187 + SetI ASST 2187 2183 . . 2186 2189 + Csp6I GTAC 2186 2188 . . 2186 2189 + RsaI GTAC 2187 2187 . . 2191 2204 + AjuI GAANNNNNNNTTGG 2183 2178 2215 2210 2198 2206 + DraIII CACNNNGTG 2203 2200 . . 2212 2215 + SetI ASST 2215 2211 . . 2233 2236 + TspEI AATT 2232 2236 . . 2238 2244 + TspRI CASTGNN 2244 2235 . . 2273 2277 + MboII GAAGA 2285 2284 . . 2276 2280 + MboII GAAGA 2288 2287 . . 2279 2283 + PleI GAGTC 2287 2288 . . 2279 2283 + MlyI GAGTC 2288 2288 . . 2279 2283 + HinfI GANTC 2279 2282 . . 2276 2281 - Ksp632I* CTCTTC 2271 2274 . . 2282 2286 - MboII GAAGA 2274 2273 . . 2281 2286 - BbvII* GAAGAC 2274 2278 . . 2279 2289 - Hin4I GAYNNNNNVTC 2302 2297 2270 2265 2298 2303 + StyI CCWWGG 2298 2302 . . 2298 2303 + SecI* CCNNGG 2298 2302 . . 2301 2304 + SetI ASST 2304 2300 . . 2306 2310 + Tsp45I GTSAC 2305 2310 . . 2306 2310 + MaeIII GTNAC 2305 2310 . . 2315 2319 + MaeIII GTNAC 2314 2319 . . 2313 2318 - TsoI TARCCA 2303 2301 . . 2319 2324 - BtgZI GCGATG 2304 2308 . . 2331 2337 + TspRI CASTGNN 2337 2328 . . 2332 2336 + BsrI ACTGG 2337 2335 . . 2338 2341 + MseI TTAA 2338 2340 . . 2343 2349 + TspRI CASTGNN 2349 2340 . . 2344 2348 + BsrI ACTGG 2349 2347 . . 2357 2363 + TspRI CASTGNN 2363 2354 . . 2359 2364 + Hpy166II GTNNAC 2361 2361 . . 2360 2365 + TatI WGTACW 2360 2364 . . 2360 2365 + Bsp1407I TGTACA 2360 2364 . . 2361 2366 + Hpy166II GTNNAC 2363 2363 . . 2361 2364 + Csp6I GTAC 2361 2363 . . 2361 2364 + RsaI GTAC 2362 2362 . . 2367 2372 + Hpy178III* TCNNGA 2368 2370 . . 2366 2371 - Eco57MI CTGRAG 2351 2349 . . 2366 2371 - GsuI CTGGAG 2351 2349 . . 2376 2380 + MboII GAAGA 2388 2387 . . 2377 2387 + FalI AAGNNNNNCTT 2368 2363 2400 2395 2379 2382 + DpnI GATC 2380 2380 . . 2379 2382 + BstKTI GATC 2381 2379 . . 2379 2382 + MboI GATC 2378 2382 . . 2381 2384 + TaqI TCGA 2381 2383 . . 2385 2389 + DdeI CTNAG 2385 2388 . . 2377 2387 - FalI AAGNNNNNCTT 2400 2395 2368 2363 2388 2391 + SetI ASST 2391 2387 . . 2388 2391 + CviJI RGCY 2389 2389 . . 2388 2391 + AluI AGCT 2389 2389 . . 2396 2400 + Hpy188I TCNGA 2398 2397 . . 2401 2405 + MboII GAAGA 2413 2412 . . 2416 2419 + MseI TTAA 2416 2418 . . 2432 2436 + Tsp45I GTSAC 2431 2436 . . 2432 2436 + MaeIII GTNAC 2431 2436 . . 2433 2438 + Hpy178III* TCNNGA 2434 2436 . . 2442 2445 + SetI ASST 2445 2441 . . 2443 2447 + HphI GGTGA 2455 2454 . . 2456 2460 + Tsp4CI* ACNGT 2458 2457 . . 2462 2465 + SetI ASST 2465 2461 . . 2462 2465 + CviJI RGCY 2463 2463 . . 2462 2465 + AluI AGCT 2463 2463 . . 2468 2473 - TsoI TARCCA 2458 2456 . . 2483 2488 + HindIII AAGCTT 2483 2487 . . 2484 2487 + SetI ASST 2487 2483 . . 2484 2487 + CviJI RGCY 2485 2485 . . 2484 2487 + AluI AGCT 2485 2485 . . 2489 2492 + MaeI CTAG 2489 2491 . . 2492 2502 + MwoI GCNNNNNNNGC 2498 2495 . . 2506 2511 + MfeI CAATTG 2506 2510 . . 2507 2510 + TspEI AATT 2506 2510 . . 2514 2517 + CviJI RGCY 2515 2515 . . 2518 2528 + Hin4I GAYNNNNNVTC 2509 2504 2541 2536 2527 2531 + Hpy188I TCNGA 2529 2528 . . 2528 2533 + Eco57MI CTGRAG 2549 2547 . . 2528 2533 + Eco57I CTGAAG 2549 2547 . . 2533 2538 + Cac8I GCNNGC 2535 2535 . . 2568 2573 + SecI* CCNNGG 2568 2572 . . 2569 2573 + BssKI CCNGG 2568 2573 . . 2569 2573 + BseBI CCWGG 2570 2571 . . 2569 2573 + ScrFI CCNGG 2570 2571 . . 2569 2573 + EcoRII CCWGG 2568 2573 . . 2572 2575 + CviJI RGCY 2573 2573 . . 2572 2575 + HaeIII GGCC 2573 2573 . . 2575 2578 + FatI CATG 2574 2578 . . 2575 2578 + NlaIII CATG 2578 2574 . . 2575 2578 + CviAII CATG 2575 2577 . . 2586 2589 + SetI ASST 2589 2585 . . 2596 2600 + BccI CCATC 2604 2605 . . 2599 2603 - MboII GAAGA 2591 2590 . . 2608 2616 + Tth111I GACNNNGTC 2611 2612 . . 2609 2613 + Tsp4CI* ACNGT 2611 2610 . . 2614 2618 + Tsp45I GTSAC 2613 2618 . . 2614 2618 + MaeIII GTNAC 2613 2618 . . 2618 2621 + AciI CCGC 2618 2620 . . 2615 2619 - HphI GGTGA 2607 2606 . . 2623 2628 + Hpy178III* TCNNGA 2624 2626 . . 2627 2637 + Hin4I GAYNNNNNVTC 2618 2613 2650 2645 2627 2637 - Hin4I GAYNNNNNVTC 2650 2645 2618 2613 2654 2657 + TaqI TCGA 2654 2656 . . 2675 2679 + TspDTI ATGAA 2690 2688 . . 2678 2683 + HindIII AAGCTT 2678 2682 . . 2679 2682 + SetI ASST 2682 2678 . . 2679 2682 + CviJI RGCY 2680 2680 . . 2679 2682 + AluI AGCT 2680 2680 . . 2691 2697 + TspRI CASTGNN 2697 2688 . . 2695 2699 + HgaI GACGC 2704 2709 . . 2699 2703 + DdeI CTNAG 2699 2702 . . 2702 2705 + SetI ASST 2705 2701 . . 2698 2704 - Bpu10I CCTNAGC 2699 2702 . . 2711 2715 + TfiI GAWTC 2711 2714 . . 2711 2715 + HinfI GANTC 2711 2714 . . 2720 2723 + TaqI TCGA 2720 2722 . . 2729 2733 + Tsp4CI* ACNGT 2731 2730 . . 2731 2735 + HphI GGTGA 2743 2742 . . 2738 2741 + SetI ASST 2741 2737 . . 2739 2744 + KpnI GGTACC 2743 2739 . . 2739 2744 + NlaIV GGNNCC 2741 2741 . . 2739 2744 + HgiCI* GGYRCC 2739 2743 . . 2739 2744 + Acc65I GGTACC 2739 2743 . . 2740 2743 + Csp6I GTAC 2740 2742 . . 2740 2743 + RsaI GTAC 2741 2741 . . 2746 2750 + Tsp4CI* ACNGT 2748 2747 . . 2752 2757 + SduI GDGCHC 2756 2752 . . 2752 2757 + HgiAI* GWGCWC 2756 2752 . . 2755 2764 + MslI CAYNNNNRTG 2759 2759 . . 2758 2762 + MboII GAAGA 2770 2769 . . 2764 2768 + HinfI GANTC 2764 2767 . . 2764 2768 - PleI GAGTC 2758 2759 . . 2764 2768 - MlyI GAGTC 2758 2758 . . 2770 2774 + BlsI GCNGC 2772 2771 . . 2770 2774 + BisI GCNGC 2771 2772 . . 2770 2774 + TseI GCWGC 2770 2773 . . 2768 2773 - EcoP15I CAGCAG 2740 2742 . . 2770 2774 - BbvI GCAGC 2757 2761 . . 2771 2776 - EcoP15I CAGCAG 2743 2745 . . 2786 2789 + MseI TTAA 2786 2788 . . 2798 2801 + TaiI ACGT 2801 2797 . . 2798 2801 + SetI ASST 2801 2797 . . 2798 2801 + MaeII ACGT 2798 2800 . . 2804 2808 + Hin4II* CCTTC 2814 2813 . . 2810 2813 + CviJI RGCY 2811 2811 . . 2813 2818 + MfeI CAATTG 2813 2817 . . 2814 2817 + TspEI AATT 2813 2817 . . 2822 2825 + SetI ASST 2825 2821 . . 2842 2847 + AclI AACGTT 2843 2845 . . 2843 2846 + TaiI ACGT 2846 2842 . . 2843 2846 + SetI ASST 2846 2842 . . 2843 2846 + MaeII ACGT 2843 2845 . . 2849 2852 + TspEI AATT 2848 2852 . . 2851 2854 + MseI TTAA 2851 2853 . . 2869 2874 + Hpy166II GTNNAC 2871 2871 . . 2872 2878 + TspRI CASTGNN 2878 2869 . . 2873 2877 + BsrI ACTGG 2878 2876 . . 2873 2878 + BfiI ACTGGG 2883 2882 . . 2877 2880 + CviJI RGCY 2878 2878 . . 2896 2900 + HphI GGTGA 2908 2907 . . 2897 2901 + Tsp45I GTSAC 2896 2901 . . 2897 2901 + MaeIII GTNAC 2896 2901 . . 2899 2909 + Hin4I GAYNNNNNVTC 2890 2885 2922 2917 2899 2909 + Eam1105I GACNNNNNGTC 2904 2903 . . 2901 2905 - FokI GGATG 2887 2891 . . 2901 2905 - BseGI GGATG 2900 2898 . . 2908 2913 + BetI* WCCGGW 2908 2912 . . 2899 2909 - Hin4I GAYNNNNNVTC 2922 2917 2890 2885 2909 2912 + HpaII CCGG 2909 2911 . . 2917 2920 + MseI TTAA 2917 2919 . . 2928 2931 + SetI ASST 2931 2927 . . 2928 2931 + CviJI RGCY 2929 2929 . . 2928 2931 + AluI AGCT 2929 2929 . . 2929 2939 + MwoI GCNNNNNNNGC 2935 2932 . . 2930 2934 + DdeI CTNAG 2930 2933 . . 2933 2936 + SetI ASST 2936 2932 . . 2930 2934 - BspCNI CTCAG 2922 2920 . . 2930 2934 - BseMII CTCAG 2921 2919 . . 2932 2935 - MnlI CCTC 2925 2924 . . 2929 2935 - Bpu10I CCTNAGC 2930 2933 . . 2929 2935 - BbvCI CCTCAGC 2930 2933 . . 2937 2940 + CviJI RGCY 2938 2938 . . 2938 2942 + TauI GCSGC 2941 2938 . . 2938 2948 + MwoI GCNNNNNNNGC 2944 2941 . . 2938 2942 + BlsI GCNGC 2940 2939 . . 2938 2942 + BisI GCNGC 2939 2940 . . 2939 2942 + AciI CCGC 2939 2941 . . 2941 2951 + MwoI GCNNNNNNNGC 2947 2944 . . 2946 2949 + CviJI RGCY 2947 2947 . . 2947 2951 + TauI GCSGC 2950 2947 . . 2947 2951 + BlsI GCNGC 2949 2948 . . 2947 2951 + BisI GCNGC 2948 2949 . . 2948 2953 + NspBII* CMGCKG 2950 2950 . . 2948 2951 + AciI CCGC 2948 2950 . . #--------------------------------------- # # # AatII: # [GACGTC] # 1409 1555 # # Acc65I: # [GGTACC] # 225 722 2017 # # AccI: # [GTMKAC] # 171 240 333 453 1767 # # AciI: # [CCGC] # 9 16 87 150 168 321 # 347 402 687 777 # # AclI: # [AACGTT] # 121 666 2177 # # AcyI: # [GRCGYC] # 1406 1558 # # AgeI: # [ACCGGT] # 300 678 937 1049 # # AhaIII*: # [TTTAAA] # 764 2200 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 502 749 1649 # # AluI: # [AGCT] # 22 24 30 35 48 61 # 74 88 107 195 213 225 # 249 312 340 399 542 # # AlwNI: # [CAGNNNCTG] # 24 393 776 1771 # # ApoI: # [RAATTY] # 127 153 191 267 447 514 # 1265 # # AsuI*: # [GGNCC] # 874 2090 # # AvaII: # [GGWCC] # 874 2090 # # BaeI: # [ACNNNNGTAYC] # 33 33 416 1020 1462 # # BalI: # [TGGCCA] # 966 1998 # # BbvCI: # [CCTCAGC] # 34 2930 # # BbvI: # [GCAGC] # 12 207 858 942 945 # # BbvII*: # [GAAGAC] # 79 466 690 1729 # # BccI: # [CCATC] # 56 77 94 146 248 278 # 360 786 919 # # Bce83I*: # [CTTGAG] # 201 2763 # # BceAI: # [ACGGC] # 359 661 861 1083 # # BclI: # [TGATCA] # 1377 1587 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 34 34 # 102 1052 1742 # # BetI*: # [WCCGGW] # 56 300 678 937 993 # # BfiI: # [ACTGGG] # 81 2883 # # BglI: # [GCCNNNNNGGC] # 1222 1742 # # BglII: # [AGATCT] # 37 971 1956 # # BinI*: # [GGATC] # 439 2525 # # BisI: # [GCNGC] # 9 12 12 16 168 207 # 665 930 945 # # BlsI: # [GCNGC] # 9 12 12 15 168 207 # 666 930 945 # # BmgT120I: # [GGNCC] # 873 2091 # # Bpu10I: # [CCTNAGC] # 34 231 2699 # # BsaXI: # [ACNNNNNCTCC] # 30 1383 1551 # # BseBI: # [CCWGG] # 387 394 653 1530 # # BseGI: # [GGATG] # 64 2900 # # BseMII: # [CTCAG] # 43 485 2436 # # BseSI: # [GKGCMC] # 1155 1809 # # BsiYI*: # [CCNNNNNNNGG] # 1388 1576 # # BslFI: # [GGGAC] # 177 2787 # # BsmAI: # [GTCTC] # 180 1099 1685 # # BsmI: # [GAATGC] # 31 635 944 1354 # # Bsp1407I: # [TGTACA] # 604 2360 # # BspCNI: # [CTCAG] # 42 484 2438 # # BspHI: # [TCATGA] # 395 629 862 1078 # # BsrBI: # [CCGCTC] # 499 2465 # # BsrDI: # [GCAATG] # 943 2021 # # BsrI: # [ACTGG] # 12 86 195 377 529 1765 # # BssKI: # [CCNGG] # 387 396 651 1530 # # BssNAI: # [GTATAC] # 241 453 2270 # # BstEII: # [GGTNACC] # 1446 1518 # # BstKTI: # [GATC] # 40 385 407 432 501 583 # 616 # # BtgZI: # [GCGATG] # 660 2304 # # BtsI: # [GCAGTG] # 1318 1646 # # Cac8I: # [GCNNGC] # 429 876 1659 # # Cfr10I: # [RCCGGY] # 300 678 937 1049 # # CfrI: # [YGGCCR] # 902 964 1098 # # ClaI: # [ATCGAT] # 320 744 1900 # # Csp6I: # [GTAC] # 12 33 126 168 175 224 # 258 379 396 507 686 # # CspCI: # [CAANNNNNGTGG] # 35 1294 1635 # # CviAII: # [CATG] # 17 50 89 119 306 389 # 494 638 862 # # CviJI: # [RGCY] # 5 9 9 9 12 17 # 22 24 30 30 30 40 # 45 48 51 58 61 67 # 67 71 74 88 95 96 # 105 107 107 112 131 141 # 181 213 228 250 331 # # CviRI*: # [TGCA] # 3 28 75 117 381 387 # 1973 # # DdeI: # [CTNAG] # 6 34 42 45 61 162 # 231 314 429 1640 # # DpnI: # [GATC] # 39 385 407 432 501 584 # 616 # # DraII: # [RGGNCCY] # 874 2090 # # DraIII: # [CACNNNGTG] # 761 2203 # # Eam1105I: # [GACNNNNNGTC] # 60 2904 # # Eco57I: # [CTGAAG] # 143 415 561 606 1239 # # Eco57MI: # [CTGRAG] # 143 198 363 415 492 606 # 747 # # EcoP15I: # [CAGCAG] # 3 221 954 1786 # # EcoRI: # [GAATTC] # 841 2123 # # EcoRII: # [CCWGG] # 387 396 651 1530 # # EspI*: # [GCTNAGC] # 477 2487 # # FalI: # [AAGNNNNNCTT] # 0 0 32 564 2368 # # FatI: # [CATG] # 16 50 89 119 306 390 # 494 638 862 # # FokI: # [GGATG] # 77 2887 # # GsuI: # [CTGGAG] # 613 635 1716 # # HaeIII: # [GGCC] # 250 321 331 391 705 966 # # HgaI: # [GACGC] # 260 910 1794 # # HgiAI*: # [GWGCWC] # 208 285 2471 # # HgiCI*: # [GGYRCC] # 213 225 564 722 1240 # # HgiJII*: # [GRGCYC] # 601 2363 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 29 32 32 32 32 32 # 32 42 77 207 236 240 # 1941 # # Hin4II*: # [CCTTC] # 9 61 69 150 154 189 # 217 244 308 346 477 740 # # HindII: # [GTYRAC] # 494 1137 1333 # # HindIII: # [AAGCTT] # 59 195 286 2424 # # HinfI: # [GANTC] # 13 24 34 53 86 95 # 125 153 162 199 200 246 # 290 355 432 497 # # HpaII: # [CCGG] # 55 300 678 938 993 # # HphI: # [GGTGA] # 56 136 151 152 165 199 # 279 305 525 996 # # Hpy166II: # [GTNNAC] # 2 19 29 61 93 123 # 241 272 433 453 508 730 # # Hpy178III*: # [TCNNGA] # 10 51 57 59 66 76 # 164 174 190 321 340 344 # 481 631 # # Hpy188I: # [TCNGA] # 9 18 30 30 37 57 # 108 114 131 204 214 386 # 391 435 800 # # KpnI: # [GGTACC] # 221 722 2021 # # Ksp632I*: # [CTCTTC] # 606 693 1665 # # MaeI: # [CTAG] # 21 97 439 457 475 594 # 881 # # MaeII: # [ACGT] # 45 57 121 621 714 1406 # # MaeIII: # [GTNAC] # 9 13 54 68 117 182 # 198 283 648 662 730 # # MboI: # [GATC] # 37 385 407 432 501 586 # 616 # # MboII: # [GAAGA] # 3 3 3 4 11 25 # 31 39 65 84 100 163 # 178 179 194 231 254 325 # 401 671 # # MfeI: # [CAATTG] # 151 307 405 2101 # # MlyI: # [GAGTC] # 206 470 1081 1207 # # MmeI: # [TCCRAC] # 526 2438 # # MnlI: # [CCTC] # 39 42 65 179 199 210 # 333 437 1460 # # MseI: # [TTAA] # 36 41 47 51 52 65 # 66 78 84 114 148 203 # 289 370 396 450 474 # # MslI: # [CAYNNNNRTG] # 205 341 616 633 1169 # # MwoI: # [GCNNNNNNNGC] # 3 9 9 9 12 12 # 17 114 126 168 279 435 # 437 661 673 # # NlaIII: # [CATG] # 20 50 89 119 306 386 # 494 638 862 # # NlaIV: # [GGNNCC] # 45 54 168 183 223 235 # 510 722 824 # # NspBII*: # [CMGCKG] # 14 667 816 1467 # # OliI: # [CACNNNNGTG] # 1169 1795 # # PflMI: # [CCANNNNNTGG] # 1388 1576 # # PleI: # [GAGTC] # 206 471 1081 1206 # # PpuMI: # [RGGWCCY] # 874 2090 # # PsrI: # [GAACNNNNNNTAC] # 32 816 2116 # # PstI: # [CTGCAG] # 876 2088 # # PvuII: # [CAGCTG] # 1481 1483 # # RsaI: # [GTAC] # 12 33 126 168 175 223 # 258 379 396 507 687 # # SapI: # [GCTCTTC] # 606 2358 # # ScrFI: # [CCNGG] # 387 394 653 1530 # # SduI: # [GDGCHC] # 208 285 316 947 1208 # # SecI*: # [CCNNGG] # 270 396 856 1442 # # SetI: # [ASST] # 4 5 5 7 7 9 # 9 10 12 13 14 15 # 20 21 22 23 24 24 # 24 24 26 28 28 34 # 36 36 37 39 39 39 # 41 48 54 54 60 63 # 63 70 85 87 88 88 # 89 93 96 96 102 102 # 148 273 530 # # SfaNI: # [GCATC] # 54 111 213 267 291 579 # 1449 # # SfeI*: # [CTRYAG] # 872 2092 # # SmlI: # [CTYRAG] # 8 172 2784 # # SspI: # [AATATT] # 760 2204 # # StyI: # [CCWWGG] # 666 856 1442 # # TaiI: # [ACGT] # 45 57 118 621 714 1409 # # TaqI: # [TCGA] # 12 57 66 147 244 273 # 279 320 432 459 675 # # TaqII: # [CACCCA] # 184 823 1957 # # TatI: # [WGTACW] # 604 2360 # # TauI: # [GCSGC] # 9 14 667 1098 1176 # # TfiI: # [GAWTC] # 13 24 95 120 153 162 # 199 246 253 290 355 497 # 557 # # TseI: # [GCWGC] # 12 194 871 942 945 # # TsoI: # [TARCCA] # 27 155 183 506 603 678 # 812 # # Tsp45I: # [GTSAC] # 68 126 182 198 283 648 # 1459 # # Tsp4CI*: # [ACNGT] # 17 25 53 57 86 118 # 120 153 201 216 292 1626 # # TspDTI: # [ATGAA] # 5 66 267 274 322 335 # 787 908 # # TspEI: # [AATT] # 5 20 21 24 28 32 # 35 38 45 50 52 57 # 81 98 116 120 127 131 # 265 267 274 307 345 426 # # TspGWI: # [ACGGA] # 226 1069 1669 # # TspRI: # [CASTGNN] # 12 14 75 86 93 181 # 252 264 334 1653 # # Tth111I: # [GACNNNGTC] # 353 2611 # # VspI: # [ATTAAT] # 1391 1573 # # XbaI: # [TCTAGA] # 553 881 1530 # # XhoII: # [RGATCY] # 37 407 971 1549 # # XmnI: # [GAANNNNTTC] # 118 1070 1776 # # ZraI: # [GACGTC] # 1407 1557 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2964 # Reported_sequences: 1 # Reported_hitcount: 694 #---------------------------------------