Restriction Fragments for KEL2/YGR238C



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The currently selected gene/sequence is :

KEL2/YGR238C on chromosome VII from coordinates 968687 to 966039.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.20021.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + KpnI GGTACC 7 3 . . 3 8 + NlaIV GGNNCC 5 5 . . 3 8 + HgiCI* GGYRCC 3 7 . . 3 8 + Acc65I GGTACC 3 7 . . 4 7 + Csp6I GTAC 4 6 . . 4 7 + RsaI GTAC 5 5 . . 6 9 + SetI ASST 9 5 . . 14 17 + SetI ASST 17 13 . . 14 17 + CviJI RGCY 15 15 . . 14 17 + AluI AGCT 15 15 . . 26 29 + SetI ASST 29 25 . . 27 32 + KpnI GGTACC 31 27 . . 27 32 + NlaIV GGNNCC 29 29 . . 27 32 + HgiCI* GGYRCC 27 31 . . 27 32 + Acc65I GGTACC 27 31 . . 28 31 + Csp6I GTAC 28 30 . . 28 31 + RsaI GTAC 29 29 . . 30 33 + SetI ASST 33 29 . . 34 38 + TspGWI ACGGA 49 47 . . 42 48 + PpuMI RGGWCCY 43 46 . . 42 48 + DraII RGGNCCY 43 46 . . 42 46 + BslFI GGGAC 56 60 . . 42 47 + NlaIV GGNNCC 44 44 . . 43 47 + BmgT120I GGNCC 44 45 . . 43 47 + AvaII GGWCC 43 46 . . 43 47 + AsuI* GGNCC 43 46 . . 45 48 + SetI ASST 48 44 . . 46 50 + Hin4II* CCTTC 56 55 . . 51 55 + Hpy188I TCNGA 53 52 . . 61 64 + Hin6I GCGC 61 63 . . 61 64 + GlaI GCGC 62 62 . . 61 64 + HhaI GCGC 63 61 . . 68 73 + Hpy166II GTNNAC 70 70 . . 70 73 + Csp6I GTAC 70 72 . . 70 73 + RsaI GTAC 71 71 . . 99 102 + TaqI TCGA 99 101 . . 110 113 + FnuDII* CGCG 111 111 . . 118 122 + MaeIII GTNAC 117 122 . . 123 126 + SetI ASST 126 122 . . 127 137 + BsaXI ACNNNNNCTCC 117 114 147 144 132 135 + SetI ASST 135 131 . . 133 136 + MnlI CCTC 143 142 . . 149 152 - MnlI CCTC 142 141 . . 152 162 + MwoI GCNNNNNNNGC 158 155 . . 161 164 + Hin6I GCGC 161 163 . . 161 164 + GlaI GCGC 162 162 . . 161 164 + HhaI GCGC 163 161 . . 162 165 + FnuDII* CGCG 163 163 . . 163 173 + MwoI GCNNNNNNNGC 169 166 . . 164 168 + Hpy99I CGWCG 168 163 . . 165 170 + SalI GTCGAC 165 169 . . 165 170 + Hpy166II GTNNAC 167 167 . . 165 170 + HindII GTYRAC 167 167 . . 165 170 + AccI GTMKAC 166 168 . . 166 169 + TaqI TCGA 166 168 . . 163 167 - HgaI GACGC 152 157 . . 172 178 + SapI GCTCTTC 179 182 . . 173 178 + Ksp632I* CTCTTC 179 182 . . 174 178 - MboII GAAGA 166 165 . . 178 183 + SecI* CCNNGG 178 182 . . 179 183 + BssKI CCNGG 178 183 . . 179 183 + BseBI CCWGG 180 181 . . 179 183 + ScrFI CCNGG 180 181 . . 179 183 + EcoRII CCWGG 178 183 . . 182 187 + Eco31I GGTCTC 188 192 . . 183 187 + BsmAI GTCTC 188 192 . . 186 191 + Hpy178III* TCNNGA 187 189 . . 185 190 - Eco57MI CTGRAG 170 168 . . 185 190 - GsuI CTGGAG 170 168 . . 243 247 - TspGWI ACGGA 233 231 . . 258 261 + TaqI TCGA 258 260 . . 264 269 + Ksp632I* CTCTTC 270 273 . . 269 272 + MnlI CCTC 279 278 . . 265 269 - MboII GAAGA 257 256 . . 280 284 + MaeIII GTNAC 279 284 . . 304 308 + Tsp45I GTSAC 303 308 . . 304 308 + MaeIII GTNAC 303 308 . . 307 312 + Cfr10I RCCGGY 307 311 . . 307 312 + BetI* WCCGGW 307 311 . . 307 312 + AgeI ACCGGT 307 311 . . 308 311 + HpaII CCGG 308 310 . . 311 316 + Hpy166II GTNNAC 313 313 . . 318 323 + Hpy178III* TCNNGA 319 321 . . 322 325 + DpnI GATC 323 323 . . 322 325 + BstKTI GATC 324 322 . . 322 325 + MboI GATC 321 325 . . 339 343 + FokI GGATG 352 356 . . 339 343 + BseGI GGATG 345 343 . . 354 357 + CviJI RGCY 355 355 . . 355 359 + TauI GCSGC 358 355 . . 355 365 + MwoI GCNNNNNNNGC 361 358 . . 355 359 + BlsI GCNGC 357 356 . . 355 359 + BisI GCNGC 356 357 . . 356 359 + AciI CCGC 356 358 . . 363 366 + FnuDII* CGCG 364 364 . . 366 370 + FokI GGATG 379 383 . . 366 370 + BseGI GGATG 372 370 . . 364 367 - AciI CCGC 364 366 . . 371 374 + Csp6I GTAC 371 373 . . 367 371 - BccI CCATC 361 362 . . 371 374 + RsaI GTAC 372 372 . . 376 379 + SetI ASST 379 375 . . 376 379 + CviJI RGCY 377 377 . . 376 379 + AluI AGCT 377 377 . . 392 396 + TfiI GAWTC 392 395 . . 392 396 + HinfI GANTC 392 395 . . 394 399 + ClaI ATCGAT 395 397 . . 395 398 + TaqI TCGA 395 397 . . 397 406 + BsaBI GATNNNNATC 401 401 . . 402 407 + BclI TGATCA 402 406 . . 403 406 + DpnI GATC 404 404 . . 403 406 + BstKTI GATC 405 403 . . 403 406 + MboI GATC 402 406 . . 414 417 + SetI ASST 417 413 . . 415 418 + MnlI CCTC 425 424 . . 420 430 + BsiYI* CCNNNNNNNGG 426 423 . . 421 431 + BsiYI* CCNNNNNNNGG 427 424 . . 429 433 + BslFI GGGAC 443 447 . . 432 437 + NspI RCATGY 436 432 . . 433 436 + FatI CATG 432 436 . . 433 436 + NlaIII CATG 436 432 . . 433 436 + CviAII CATG 433 435 . . 437 440 + MnlI CCTC 447 446 . . 449 452 - AciI CCGC 449 451 . . 488 491 + SetI ASST 491 487 . . 488 491 + CviJI RGCY 489 489 . . 488 491 + AluI AGCT 489 489 . . 498 511 + AjuI GAANNNNNNNTTGG 490 485 522 517 500 504 + TspGWI ACGGA 515 513 . . 504 508 + Tsp4CI* ACNGT 506 505 . . 510 514 + FokI GGATG 523 527 . . 510 514 + BseGI GGATG 516 514 . . 511 520 + BsaBI GATNNNNATC 515 515 . . 507 512 - MmeI TCCRAC 488 486 . . 517 520 + DpnI GATC 518 518 . . 517 520 + BstKTI GATC 519 517 . . 517 526 + BsaBI GATNNNNATC 521 521 . . 517 520 + MboI GATC 516 520 . . 532 537 + SspI AATATT 534 534 . . 535 540 + VspI ATTAAT 536 538 . . 536 539 + MseI TTAA 536 538 . . 538 541 + TspEI AATT 537 541 . . 543 548 + PsiI TTATAA 545 545 . . 549 554 + Hpy166II GTNNAC 551 551 . . 551 555 + BmgT120I GGNCC 552 553 . . 551 555 + AvaII GGWCC 551 554 . . 551 555 + AsuI* GGNCC 551 554 . . 564 567 + SetI ASST 567 563 . . 570 575 + McrI* CGRYCG 573 571 . . 570 575 + XmaIII* CGGCCG 570 574 . . 570 575 + CfrI YGGCCR 570 574 . . 571 574 + CviJI RGCY 572 572 . . 571 574 + HaeIII GGCC 572 572 . . 572 576 - BceAI ACGGC 557 559 . . 578 581 + CviJI RGCY 579 579 . . 578 581 + HaeIII GGCC 579 579 . . 579 583 - BceAI ACGGC 564 566 . . 587 597 + MwoI GCNNNNNNNGC 593 590 . . 589 592 + SetI ASST 592 588 . . 591 594 + Csp6I GTAC 591 593 . . 591 594 + RsaI GTAC 592 592 . . 587 592 - BspMI ACCTGC 578 582 . . 593 597 + BceAI ACGGC 609 611 . . 594 599 + CfrI YGGCCR 594 598 . . 595 598 + CviJI RGCY 596 596 . . 595 598 + HaeIII GGCC 596 596 . . 602 607 + ApoI RAATTY 602 606 . . 603 606 + TspEI AATT 602 606 . . 612 615 + TspEI AATT 611 615 . . 616 621 + Cac8I GCNNGC 618 618 . . 616 621 + BmtI GCTAGC 620 616 . . 616 621 + NheI GCTAGC 616 620 . . 617 620 + MaeI CTAG 617 619 . . 620 631 + AlfI GCANNNNNNTGC 609 607 643 641 629 632 + CviRI* TGCA 630 630 . . 620 631 - AlfI GCANNNNNNTGC 643 641 609 607 652 657 + EciI GGCGGA 668 666 . . 653 656 - AciI CCGC 653 655 . . 665 669 + TspDTI ATGAA 680 678 . . 677 680 + MseI TTAA 677 679 . . 682 685 + DpnI GATC 683 683 . . 682 685 + BstKTI GATC 684 682 . . 682 685 + MboI GATC 681 685 . . 697 707 + Hin4I GAYNNNNNVTC 688 683 720 715 697 707 - Hin4I GAYNNNNNVTC 720 715 688 683 710 714 - TspGWI ACGGA 700 698 . . 716 719 + CviJI RGCY 717 717 . . 716 719 + HaeIII GGCC 717 717 . . 720 725 + EcoRI GAATTC 720 724 . . 720 725 + ApoI RAATTY 720 724 . . 721 724 + TspEI AATT 720 724 . . 733 738 + ApoI RAATTY 733 737 . . 734 737 + TspEI AATT 733 737 . . 743 746 + CviJI RGCY 744 744 . . 755 758 + SetI ASST 758 754 . . 755 760 + BspMI ACCTGC 764 768 . . 760 763 + MnlI CCTC 770 769 . . 763 766 + MnlI CCTC 773 772 . . 761 766 - BseRI GAGGAG 752 750 . . 769 772 + MseI TTAA 769 771 . . 791 796 + NdeI CATATG 792 794 . . 803 806 + TspEI AATT 802 806 . . 818 821 - AciI CCGC 818 820 . . 818 822 - FauI CCCGC 811 813 . . 842 847 + BsrDI GCAATG 849 847 . . 855 858 + AciI CCGC 855 857 . . 862 865 + DpnI GATC 863 863 . . 862 865 + BstKTI GATC 864 862 . . 862 865 + MboI GATC 861 865 . . 862 866 - BinI* GGATC 856 857 . . 869 877 + DraIII CACNNNGTG 874 871 . . 871 882 + CspCI CAANNNNNGTGG 859 857 894 892 890 893 + MseI TTAA 890 892 . . 908 911 + CviJI RGCY 909 909 . . 910 913 + MnlI CCTC 920 919 . . 913 916 + AciI CCGC 913 915 . . 912 917 - EciI GGCGGA 902 900 . . 927 932 + NspI RCATGY 931 927 . . 927 932 + Cac8I GCNNGC 929 929 . . 927 932 + SphI GCATGC 931 927 . . 928 937 + MslI CAYNNNNRTG 932 932 . . 928 931 + FatI CATG 927 931 . . 928 931 + NlaIII CATG 931 927 . . 928 931 + CviAII CATG 928 930 . . 932 935 + MaeI CTAG 932 934 . . 952 955 + MseI TTAA 952 954 . . 968 971 - AciI CCGC 968 970 . . 975 980 - BciVI GTATCC 969 968 . . 994 999 + MmeI TCCRAC 1019 1017 . . 1011 1016 + AhaIII* TTTAAA 1013 1013 . . 1012 1015 + MseI TTAA 1012 1014 . . 1039 1044 + HindIII AAGCTT 1039 1043 . . 1040 1043 + SetI ASST 1043 1039 . . 1040 1043 + CviJI RGCY 1041 1041 . . 1040 1043 + AluI AGCT 1041 1041 . . 1047 1057 + BsiYI* CCNNNNNNNGG 1053 1050 . . 1051 1055 + TspDTI ATGAA 1066 1064 . . 1053 1057 - Hin4II* CCTTC 1047 1046 . . 1057 1060 - MnlI CCTC 1050 1049 . . 1065 1068 + SetI ASST 1068 1064 . . 1066 1069 + MnlI CCTC 1076 1075 . . 1067 1072 + SmlI CTYRAG 1067 1071 . . 1068 1073 + Hpy178III* TCNNGA 1069 1071 . . 1067 1072 - Bce83I* CTTGAG 1052 1050 . . 1078 1083 + Hpy178III* TCNNGA 1079 1081 . . 1090 1093 + MseI TTAA 1090 1092 . . 1093 1096 + SetI ASST 1096 1092 . . 1096 1099 + MseI TTAA 1096 1098 . . 1099 1103 + TspDTI ATGAA 1114 1112 . . 1101 1105 + MboII GAAGA 1113 1112 . . 1111 1116 + HindIII AAGCTT 1111 1115 . . 1112 1115 + SetI ASST 1115 1111 . . 1112 1115 + CviJI RGCY 1113 1113 . . 1112 1115 + AluI AGCT 1113 1113 . . 1122 1125 + FatI CATG 1121 1125 . . 1122 1125 + NlaIII CATG 1125 1121 . . 1122 1125 + CviAII CATG 1122 1124 . . 1124 1129 - TaqII CACCCA 1114 1112 . . 1147 1152 + Cac8I GCNNGC 1149 1149 . . 1150 1153 + CviJI RGCY 1151 1151 . . 1156 1161 + SspI AATATT 1158 1158 . . 1161 1166 + Hpy178III* TCNNGA 1162 1164 . . 1165 1168 + DpnI GATC 1166 1166 . . 1165 1168 + BstKTI GATC 1167 1165 . . 1165 1168 + MboI GATC 1164 1168 . . 1177 1181 + Hpy188I TCNGA 1179 1178 . . 1178 1186 + AlwNI CAGNNNCTG 1183 1180 . . 1175 1180 - Eco57MI CTGRAG 1160 1158 . . 1175 1180 - Eco57I CTGAAG 1160 1158 . . 1188 1193 + StyI CCWWGG 1188 1192 . . 1188 1193 + SecI* CCNNGG 1188 1192 . . 1195 1207 + BarI GAAGNNNNNNTAC 1187 1182 1219 1214 1195 1199 - Hin4II* CCTTC 1189 1188 . . 1206 1210 + Tsp4CI* ACNGT 1208 1207 . . 1209 1214 + Hpy166II GTNNAC 1211 1211 . . 1209 1214 + BssNAI GTATAC 1211 1211 . . 1209 1214 + AccI GTMKAC 1210 1212 . . 1213 1216 + SetI ASST 1216 1212 . . 1215 1219 + DdeI CTNAG 1215 1218 . . 1219 1229 + Hin4I GAYNNNNNVTC 1210 1205 1242 1237 1219 1229 - Hin4I GAYNNNNNVTC 1242 1237 1210 1205 1238 1241 + SetI ASST 1241 1237 . . 1238 1241 + CviJI RGCY 1239 1239 . . 1238 1241 + AluI AGCT 1239 1239 . . 1245 1250 + SecI* CCNNGG 1245 1249 . . 1246 1250 + BssKI CCNGG 1245 1250 . . 1246 1250 + BseBI CCWGG 1247 1248 . . 1246 1250 + ScrFI CCNGG 1247 1248 . . 1246 1250 + EcoRII CCWGG 1245 1250 . . 1261 1265 + TfiI GAWTC 1261 1264 . . 1261 1265 + HinfI GANTC 1261 1264 . . 1269 1274 + NspI RCATGY 1273 1269 . . 1270 1273 + FatI CATG 1269 1273 . . 1270 1273 + NlaIII CATG 1273 1269 . . 1270 1273 + CviAII CATG 1270 1272 . . 1272 1275 + CviRI* TGCA 1273 1273 . . 1301 1305 + BssKI CCNGG 1300 1305 . . 1301 1304 + HpaII CCGG 1301 1303 . . 1301 1306 + SecI* CCNNGG 1301 1305 . . 1301 1305 + ScrFI CCNGG 1302 1303 . . 1301 1305 + CauII* CCSGG 1302 1303 . . 1306 1309 + CviJI RGCY 1307 1307 . . 1316 1319 + MnlI CCTC 1326 1325 . . 1319 1323 + DdeI CTNAG 1319 1322 . . 1323 1328 + Hpy166II GTNNAC 1325 1325 . . 1323 1328 + AccI GTMKAC 1324 1326 . . 1341 1346 + Hpy178III* TCNNGA 1342 1344 . . 1395 1400 + PsiI TTATAA 1397 1397 . . 1409 1413 + TspDTI ATGAA 1424 1422 . . 1411 1415 - Hin4II* CCTTC 1405 1404 . . 1416 1419 + CviJI RGCY 1417 1417 . . 1424 1428 + MaeIII GTNAC 1423 1428 . . 1430 1441 + BstXI CCANNNNNNTGG 1437 1433 . . 1432 1437 + HaeII RGCGCY 1436 1432 . . 1432 1437 + Eco47III AGCGCT 1434 1434 . . 1433 1436 + Hin6I GCGC 1433 1435 . . 1433 1436 + GlaI GCGC 1434 1434 . . 1428 1433 - Eco57MI CTGRAG 1413 1411 . . 1428 1433 - GsuI CTGGAG 1413 1411 . . 1433 1436 + HhaI GCGC 1435 1433 . . 1440 1444 + FokI GGATG 1453 1457 . . 1440 1444 + BseGI GGATG 1446 1444 . . 1465 1469 + Hpy188I TCNGA 1467 1466 . . 1474 1477 - MnlI CCTC 1467 1466 . . 1492 1496 - MboII GAAGA 1484 1483 . . 1504 1508 + TfiI GAWTC 1504 1507 . . 1504 1508 + HinfI GANTC 1504 1507 . . 1511 1515 + SfaNI GCATC 1520 1524 . . 1527 1533 + PfoI TCCNGGA 1527 1532 . . 1528 1532 + BssKI CCNGG 1527 1532 . . 1528 1531 + HpaII CCGG 1528 1530 . . 1528 1532 + ScrFI CCNGG 1529 1530 . . 1528 1532 + CauII* CCSGG 1529 1530 . . 1528 1538 + BsiYI* CCNNNNNNNGG 1534 1531 . . 1534 1538 + BsrI ACTGG 1539 1537 . . 1557 1562 + ApoI RAATTY 1557 1561 . . 1558 1561 + TspEI AATT 1557 1561 . . 1574 1577 - MnlI CCTC 1567 1566 . . 1582 1586 + Tsp4CI* ACNGT 1584 1583 . . 1583 1589 + TspRI CASTGNN 1589 1580 . . 1576 1587 - TstI CACNNNNNNTCC 1600 1595 1568 1563 1588 1592 + TfiI GAWTC 1588 1591 . . 1588 1592 + HinfI GANTC 1588 1591 . . 1591 1595 + Hpy188I TCNGA 1593 1592 . . 1594 1598 + MboII GAAGA 1606 1605 . . 1597 1607 + BplI GAGNNNNNCTC 1588 1583 1620 1615 1594 1599 - Ksp632I* CTCTTC 1589 1592 . . 1603 1606 + CviJI RGCY 1604 1604 . . 1604 1610 + SapI GCTCTTC 1611 1614 . . 1605 1610 + Ksp632I* CTCTTC 1611 1614 . . 1597 1607 - BplI GAGNNNNNCTC 1620 1615 1588 1583 1606 1610 - MboII GAAGA 1598 1597 . . 1609 1613 - MboII GAAGA 1601 1600 . . 1611 1615 - TspDTI ATGAA 1601 1599 . . 1628 1631 + FatI CATG 1627 1631 . . 1628 1631 + NlaIII CATG 1631 1627 . . 1628 1631 + CviAII CATG 1628 1630 . . 1640 1645 + EcoRI GAATTC 1640 1644 . . 1640 1645 + ApoI RAATTY 1640 1644 . . 1641 1644 + TspEI AATT 1640 1644 . . 1665 1670 + AhaIII* TTTAAA 1667 1667 . . 1666 1669 + MseI TTAA 1666 1668 . . 1670 1673 + SetI ASST 1673 1669 . . 1676 1679 + SetI ASST 1679 1675 . . 1689 1692 + TaiI ACGT 1692 1688 . . 1689 1692 + SetI ASST 1692 1688 . . 1689 1692 + MaeII ACGT 1689 1691 . . 1690 1695 + Esp3I CGTCTC 1696 1700 . . 1691 1695 + BsmAI GTCTC 1696 1700 . . 1692 1697 + Hpy178III* TCNNGA 1693 1695 . . 1694 1697 + TaqI TCGA 1694 1696 . . 1698 1701 + SetI ASST 1701 1697 . . 1698 1701 + CviJI RGCY 1699 1699 . . 1698 1701 + AluI AGCT 1699 1699 . . 1724 1727 + MaeI CTAG 1724 1726 . . 1739 1742 + TspEI AATT 1738 1742 . . 1754 1759 + Hpy178III* TCNNGA 1755 1757 . . 1760 1764 + HinfI GANTC 1760 1763 . . 1758 1762 - BsmAI GTCTC 1752 1756 . . 1760 1764 - PleI GAGTC 1754 1755 . . 1760 1764 - MlyI GAGTC 1754 1754 . . 1764 1767 + FatI CATG 1763 1767 . . 1764 1767 + NlaIII CATG 1767 1763 . . 1764 1767 + CviAII CATG 1764 1766 . . 1778 1783 + HindIII AAGCTT 1778 1782 . . 1779 1782 + SetI ASST 1782 1778 . . 1779 1782 + CviJI RGCY 1780 1780 . . 1779 1782 + AluI AGCT 1780 1780 . . 1789 1793 + TfiI GAWTC 1789 1792 . . 1789 1793 + HinfI GANTC 1789 1792 . . 1794 1799 + ApoI RAATTY 1794 1798 . . 1795 1798 + TspEI AATT 1794 1798 . . 1809 1813 + BceAI ACGGC 1825 1827 . . 1811 1814 + CviJI RGCY 1812 1812 . . 1814 1817 + MseI TTAA 1814 1816 . . 1821 1826 + XbaI TCTAGA 1821 1825 . . 1821 1826 + Hpy178III* TCNNGA 1822 1824 . . 1822 1825 + MaeI CTAG 1822 1824 . . 1826 1831 + ApoI RAATTY 1826 1830 . . 1827 1830 + TspEI AATT 1826 1830 . . 1830 1834 + Hpy188I TCNGA 1832 1831 . . 1837 1840 + SetI ASST 1840 1836 . . 1846 1850 + TfiI GAWTC 1846 1849 . . 1846 1850 + HinfI GANTC 1846 1849 . . 1849 1854 + XbaI TCTAGA 1849 1853 . . 1849 1854 + Hpy178III* TCNNGA 1850 1852 . . 1850 1853 + MaeI CTAG 1850 1852 . . 1854 1857 + TspEI AATT 1853 1857 . . 1855 1860 + VspI ATTAAT 1856 1858 . . 1856 1859 + MseI TTAA 1856 1858 . . 1863 1868 + AhaIII* TTTAAA 1865 1865 . . 1864 1867 + MseI TTAA 1864 1866 . . 1869 1874 + ApoI RAATTY 1869 1873 . . 1870 1873 + TspEI AATT 1869 1873 . . 1878 1882 - BccI CCATC 1872 1873 . . 1886 1889 + TspEI AATT 1885 1889 . . 1892 1896 + DdeI CTNAG 1892 1895 . . 1892 1896 + BspCNI CTCAG 1905 1903 . . 1892 1896 + BseMII CTCAG 1906 1904 . . 1901 1906 + Hpy166II GTNNAC 1903 1903 . . 1910 1914 + Tsp45I GTSAC 1909 1914 . . 1910 1914 + MaeIII GTNAC 1909 1914 . . 1917 1920 + TspEI AATT 1916 1920 . . 1923 1927 + TfiI GAWTC 1923 1926 . . 1923 1927 + HinfI GANTC 1923 1926 . . 1914 1927 - AjuI GAANNNNNNNTTGG 1939 1934 1907 1902 1940 1943 + MseI TTAA 1940 1942 . . 1945 1950 + NspI RCATGY 1949 1945 . . 1946 1949 + FatI CATG 1945 1949 . . 1946 1949 + NlaIII CATG 1949 1945 . . 1946 1949 + CviAII CATG 1946 1948 . . 1951 1955 + Hpy188I TCNGA 1953 1952 . . 1956 1961 + BspLU11I* ACATGT 1956 1960 . . 1956 1961 + NspI RCATGY 1960 1956 . . 1956 1961 + AflIII ACRYGT 1956 1960 . . 1957 1960 + FatI CATG 1956 1960 . . 1957 1960 + NlaIII CATG 1960 1956 . . 1957 1960 + CviAII CATG 1957 1959 . . 1960 1964 + Tsp45I GTSAC 1959 1964 . . 1960 1964 + MaeIII GTNAC 1959 1964 . . 1967 1970 + MseI TTAA 1967 1969 . . 1975 1980 + EcoRV GATATC 1977 1977 . . 1981 1986 + ClaI ATCGAT 1982 1984 . . 1982 1985 + TaqI TCGA 1982 1984 . . 1984 1987 + DpnI GATC 1985 1985 . . 1984 1987 + BstKTI GATC 1986 1984 . . 1984 1987 + MboI GATC 1983 1987 . . 1986 1991 + Hpy178III* TCNNGA 1987 1989 . . 1987 1992 + SmlI CTYRAG 1987 1991 . . 1987 1992 + Bce83I* CTTGAG 2008 2006 . . 2012 2017 + HaeII RGCGCY 2016 2012 . . 2013 2016 + Hin6I GCGC 2013 2015 . . 2013 2016 + GlaI GCGC 2014 2014 . . 2013 2016 + HhaI GCGC 2015 2013 . . 2042 2045 + CviRI* TGCA 2043 2043 . . 2063 2067 - TspDTI ATGAA 2053 2051 . . 2068 2071 + SetI ASST 2071 2067 . . 2076 2088 + AloI GAACNNNNNNTCC 2068 2063 2100 2095 2080 2084 + TfiI GAWTC 2080 2083 . . 2080 2084 + HinfI GANTC 2080 2083 . . 2102 2105 + TspEI AATT 2101 2105 . . 2107 2110 + MseI TTAA 2107 2109 . . 2113 2116 + SetI ASST 2116 2112 . . 2113 2116 + CviJI RGCY 2114 2114 . . 2113 2116 + AluI AGCT 2114 2114 . . 2123 2133 + BsiYI* CCNNNNNNNGG 2129 2126 . . 2124 2130 + TspRI CASTGNN 2130 2121 . . 2126 2131 + Hpy166II GTNNAC 2128 2128 . . 2126 2131 + SduI GDGCHC 2130 2126 . . 2126 2131 + BseSI GKGCMC 2130 2126 . . 2126 2131 + ApaLI GTGCAC 2126 2130 . . 2126 2131 + HgiAI* GWGCWC 2130 2126 . . 2127 2130 + CviRI* TGCA 2128 2128 . . 2123 2127 - BsrI ACTGG 2123 2121 . . 2130 2134 + BceAI ACGGC 2146 2148 . . 2131 2136 + CfrI YGGCCR 2131 2135 . . 2132 2135 + CviJI RGCY 2133 2133 . . 2132 2135 + HaeIII GGCC 2133 2133 . . 2136 2139 + SetI ASST 2139 2135 . . 2137 2141 + Hin4II* CCTTC 2147 2146 . . 2173 2177 + TfiI GAWTC 2173 2176 . . 2173 2177 + HinfI GANTC 2173 2176 . . 2204 2208 + TspDTI ATGAA 2219 2217 . . 2210 2213 + CviRI* TGCA 2211 2211 . . 2218 2222 + Tsp4CI* ACNGT 2220 2219 . . 2223 2228 + Cac8I GCNNGC 2225 2225 . . 2237 2240 + TspEI AATT 2236 2240 . . 2240 2243 + CviRI* TGCA 2241 2241 . . 2243 2246 + SetI ASST 2246 2242 . . 2252 2256 + DdeI CTNAG 2252 2255 . . 2252 2256 + BspCNI CTCAG 2265 2263 . . 2252 2256 + BseMII CTCAG 2266 2264 . . 2253 2257 + Hpy188I TCNGA 2255 2254 . . 2257 2262 + HindIII AAGCTT 2257 2261 . . 2258 2261 + SetI ASST 2261 2257 . . 2258 2261 + CviJI RGCY 2259 2259 . . 2258 2261 + AluI AGCT 2259 2259 . . 2261 2264 + MseI TTAA 2261 2263 . . 2267 2271 + TspDTI ATGAA 2282 2280 . . 2271 2281 + FalI AAGNNNNNCTT 2262 2257 2294 2289 2275 2279 + TfiI GAWTC 2275 2278 . . 2275 2279 + HinfI GANTC 2275 2278 . . 2271 2281 - FalI AAGNNNNNCTT 2294 2289 2262 2257 2283 2288 + Cac8I GCNNGC 2285 2285 . . 2286 2289 + CviJI RGCY 2287 2287 . . 2283 2286 - AciI CCGC 2283 2285 . . 2283 2287 - FauI CCCGC 2276 2278 . . 2290 2293 + TspEI AATT 2289 2293 . . 2297 2300 + TaqI TCGA 2297 2299 . . 2316 2320 + FokI GGATG 2329 2333 . . 2316 2320 + BseGI GGATG 2322 2320 . . 2313 2318 - MmeI TCCRAC 2294 2292 . . 2320 2323 + Hin6I GCGC 2320 2322 . . 2320 2323 + GlaI GCGC 2321 2321 . . 2320 2323 + HhaI GCGC 2322 2320 . . 2317 2321 - SfaNI GCATC 2307 2311 . . 2323 2327 + DdeI CTNAG 2323 2326 . . 2335 2339 + Hpy188I TCNGA 2337 2336 . . 2338 2348 + Hin4I GAYNNNNNVTC 2329 2324 2361 2356 2338 2342 - BccI CCATC 2332 2333 . . 2344 2347 + SetI ASST 2347 2343 . . 2344 2347 + CviJI RGCY 2345 2345 . . 2344 2347 + AluI AGCT 2345 2345 . . 2347 2350 + TaqI TCGA 2347 2349 . . 2358 2362 + BmgT120I GGNCC 2359 2360 . . 2358 2362 + AvaII GGWCC 2358 2361 . . 2358 2362 + AsuI* GGNCC 2358 2361 . . 2360 2363 + SetI ASST 2363 2359 . . 2373 2376 + CviJI RGCY 2374 2374 . . 2370 2374 - Hin4II* CCTTC 2364 2363 . . 2381 2384 + SetI ASST 2384 2380 . . 2379 2383 - HphI GGTGA 2371 2370 . . 2392 2396 + Hpy188I TCNGA 2394 2393 . . 2395 2400 + SacI GAGCTC 2399 2395 . . 2395 2400 + Ecl136II GAGCTC 2397 2397 . . 2395 2400 + SduI GDGCHC 2399 2395 . . 2395 2400 + HgiJII* GRGCYC 2399 2395 . . 2395 2400 + HgiAI* GWGCWC 2399 2395 . . 2396 2399 + SetI ASST 2399 2395 . . 2396 2399 + CviJI RGCY 2397 2397 . . 2396 2399 + AluI AGCT 2397 2397 . . 2399 2402 + TaqI TCGA 2399 2401 . . 2421 2425 + Hpy99I CGWCG 2425 2420 . . 2433 2437 - Hin4II* CCTTC 2427 2426 . . 2448 2451 + TspEI AATT 2447 2451 . . 2450 2453 + MseI TTAA 2450 2452 . . 2463 2466 + SetI ASST 2466 2462 . . 2463 2466 + CviJI RGCY 2464 2464 . . 2463 2466 + AluI AGCT 2464 2464 . . 2472 2476 + FokI GGATG 2485 2489 . . 2472 2476 + BseGI GGATG 2478 2476 . . 2481 2491 + MwoI GCNNNNNNNGC 2487 2484 . . 2494 2499 + NarI GGCGCC 2495 2497 . . 2494 2499 + KasI GGCGCC 2494 2498 . . 2494 2499 + DinI GGCGCC 2496 2496 . . 2494 2499 + HaeII RGCGCY 2498 2494 . . 2494 2499 + NlaIV GGNNCC 2496 2496 . . 2494 2499 + AcyI GRCGYC 2495 2497 . . 2494 2499 + HgiCI* GGYRCC 2494 2498 . . 2495 2498 + Hin6I GCGC 2495 2497 . . 2495 2498 + GlaI GCGC 2496 2496 . . 2495 2498 + HhaI GCGC 2497 2495 . . 2498 2502 + BccI CCATC 2506 2507 . . 2506 2509 + FatI CATG 2505 2509 . . 2506 2509 + NlaIII CATG 2509 2505 . . 2506 2509 + CviAII CATG 2506 2508 . . 2511 2516 + ApoI RAATTY 2511 2515 . . 2512 2515 + TspEI AATT 2511 2515 . . 2518 2522 - SfaNI GCATC 2508 2512 . . 2533 2537 + TspDTI ATGAA 2548 2546 . . 2547 2551 + Hpy188I TCNGA 2549 2548 . . 2554 2557 - AciI CCGC 2554 2556 . . 2559 2563 + Tsp4CI* ACNGT 2561 2560 . . 2569 2572 + TaqI TCGA 2569 2571 . . 2582 2592 + BplI GAGNNNNNCTC 2573 2568 2605 2600 2584 2588 + TfiI GAWTC 2584 2587 . . 2584 2588 + HinfI GANTC 2584 2587 . . 2589 2592 + MnlI CCTC 2599 2598 . . 2590 2595 + SmlI CTYRAG 2590 2594 . . 2582 2592 - BplI GAGNNNNNCTC 2605 2600 2573 2568 2590 2595 - Bce83I* CTTGAG 2575 2573 . . 2604 2609 + Cac8I GCNNGC 2606 2606 . . 2604 2609 + BmtI GCTAGC 2608 2604 . . 2604 2609 + NheI GCTAGC 2604 2608 . . 2605 2608 + MaeI CTAG 2605 2607 . . 2607 2610 + SetI ASST 2610 2606 . . 2607 2610 + CviJI RGCY 2608 2608 . . 2607 2610 + AluI AGCT 2608 2608 . . 2610 2615 + AhaIII* TTTAAA 2612 2612 . . 2611 2614 + MseI TTAA 2611 2613 . . 2631 2636 + Hpy178III* TCNNGA 2632 2634 . . 2633 2636 + TaqI TCGA 2633 2635 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 3 24 2622 # # AccI: # [GTMKAC] # 114 166 1044 1325 # # AciI: # [CCGC] # 8 37 55 58 85 95 # 165 204 271 356 1315 # # AcyI: # [GRCGYC] # 154 2495 # # AflIII: # [ACRYGT] # 693 1956 # # AgeI: # [ACCGGT] # 307 2342 # # AhaIII*: # [TTTAAA] # 37 198 654 747 1013 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 490 710 1385 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 609 2006 # # AloI: # [GAACNNNNNNTCC] # 32 549 2068 # # AluI: # [AGCT] # 15 41 52 67 72 81 # 86 112 126 144 145 334 # 362 460 552 # # AlwNI: # [CAGNNNCTG] # 1183 1466 # # ApaLI: # [GTGCAC] # 523 2126 # # ApoI: # [RAATTY] # 13 32 43 83 118 138 # 154 602 642 824 # # AsuI*: # [GGNCC] # 43 291 508 1807 # # AvaII: # [GGWCC] # 43 291 508 1807 # # BarI: # [GAAGNNNNNNTAC] # 32 1187 1430 # # BccI: # [CCATC] # 143 174 361 460 1511 # # Bce83I*: # [CTTGAG] # 74 567 956 1052 # # BceAI: # [ACGGC] # 7 45 321 503 557 1216 # # BciVI: # [GTATCC] # 969 1680 # # BclI: # [TGATCA] # 402 2247 # # BetI*: # [WCCGGW] # 307 2342 # # BinI*: # [GGATC] # 856 1793 # # BisI: # [GCNGC] # 356 2293 # # BlsI: # [GCNGC] # 357 2292 # # BmgT120I: # [GGNCC] # 44 290 508 1807 # # BmtI: # [GCTAGC] # 41 620 1988 # # BplI: # [GAGNNNNNCTC] # 0 0 0 0 32 32 # 44 953 1588 # # BsaBI: # [GATNNNNATC] # 6 114 401 2128 # # BsaXI: # [ACNNNNNCTCC] # 30 117 2502 # # BseBI: # [CCWGG] # 180 1067 1402 # # BseGI: # [GGATG] # 27 144 156 171 345 876 # 930 # # BseMII: # [CTCAG] # 360 383 1906 # # BseRI: # [GAGGAG] # 752 1897 # # BseSI: # [GKGCMC] # 519 2130 # # BsiYI*: # [CCNNNNNNNGG] # 1 426 481 520 595 626 # # BslFI: # [GGGAC] # 56 387 2206 # # BsmAI: # [GTCTC] # 56 188 897 1508 # # BspCNI: # [CTCAG] # 360 384 1905 # # BspLU11I*: # [ACATGT] # 693 1956 # # BspMI: # [ACCTGC] # 186 578 1885 # # BsrDI: # [GCAATG] # 849 1800 # # BsrI: # [ACTGG] # 526 584 1539 # # BssKI: # [CCNGG] # 55 178 227 1067 1122 # # BssNAI: # [GTATAC] # 1211 1438 # # BstKTI: # [GATC] # 81 114 165 180 303 324 # 663 819 # # BstXI: # [CCANNNNNNTGG] # 1212 1437 # # Cac8I: # [GCNNGC] # 43 60 220 311 321 618 # 1076 # # CauII*: # [CCSGG] # 227 1120 1302 # # Cfr10I: # [RCCGGY] # 307 2342 # # CfrI: # [YGGCCR] # 24 518 570 1537 # # ClaI: # [ATCGAT] # 395 667 1587 # # Csp6I: # [GTAC] # 4 24 42 220 301 2058 # # CspCI: # [CAANNNNNGTGG] # 35 859 1755 # # CviAII: # [CATG] # 11 136 143 148 182 194 # 358 433 495 549 # # CviJI: # [RGCY] # 7 15 17 19 22 23 # 27 28 29 32 38 41 # 58 67 68 72 81 83 # 88 95 110 112 121 126 # 132 144 165 187 302 340 # # CviRI*: # [TGCA] # 30 83 85 408 630 643 # 770 # # DdeI: # [CTNAG] # 71 104 326 360 573 1215 # # DinI: # [GGCGCC] # 153 2496 # # DpnI: # [GATC] # 81 114 165 180 303 323 # 664 819 # # DraII: # [RGGNCCY] # 43 2606 # # DraIII: # [CACNNNGTG] # 874 1775 # # EciI: # [GGCGGA] # 234 668 1747 # # Ecl136II: # [GAGCTC] # 252 2397 # # Eco31I: # [GGTCTC] # 188 2461 # # Eco47III: # [AGCGCT] # 1215 1434 # # Eco57I: # [CTGAAG] # 1160 1489 # # Eco57MI: # [CTGRAG] # 170 253 990 1236 # # EcoRI: # [GAATTC] # 720 920 1009 # # EcoRII: # [CCWGG] # 178 1067 1404 # # EcoRV: # [GATATC] # 672 1977 # # Esp3I: # [CGTCTC] # 953 1696 # # FalI: # [AAGNNNNNCTT] # 0 0 32 355 2262 # # FatI: # [CATG] # 11 136 144 148 182 194 # 358 432 495 549 # # FauI: # [CCCGC] # 373 811 1465 # # FnuDII*: # [CGCG] # 52 111 201 2285 # # FokI: # [GGATG] # 27 144 156 164 352 876 # 930 # # GlaI: # [GCGC] # 62 100 153 175 307 580 # 1272 # # GsuI: # [CTGGAG] # 170 1236 1243 # # HaeII: # [RGCGCY] # 151 482 580 1436 # # HaeIII: # [GGCC] # 7 17 121 516 572 1416 # # HgaI: # [GACGC] # 152 2497 # # HgiAI*: # [GWGCWC] # 250 269 2130 # # HgiCI*: # [GGYRCC] # 3 24 155 2467 # # HgiJII*: # [GRGCYC] # 250 2399 # # HhaI: # [GCGC] # 63 100 152 175 307 580 # 1272 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 288 490 688 1087 # # Hin4II*: # [CCTTC] # 56 63 142 216 217 222 # 742 991 # # Hin6I: # [GCGC] # 61 100 154 175 307 580 # 1272 # # HindII: # [GTYRAC] # 167 2482 # # HindIII: # [AAGCTT] # 72 392 479 667 1039 # # HinfI: # [GANTC] # 29 57 65 77 84 93 # 102 157 172 243 309 392 # 869 # # HpaII: # [CCGG] # 227 308 993 1121 # # HphI: # [GGTGA] # 278 2371 # # Hpy166II: # [GTNNAC] # 70 97 114 146 225 238 # 521 578 660 # # Hpy178III*: # [TCNNGA] # 10 17 28 62 67 83 # 132 137 180 187 351 645 # 750 # # Hpy188I: # [TCNGA] # 53 57 82 100 121 126 # 155 239 288 302 1126 # # Hpy99I: # [CGWCG] # 168 224 2257 # # KasI: # [GGCGCC] # 155 2494 # # KpnI: # [GGTACC] # 7 24 2618 # # Ksp632I*: # [CTCTTC] # 22 91 179 1038 1319 # # MaeI: # [CTAG] # 28 44 98 315 617 755 # 792 # # MaeII: # [ACGT] # 960 1689 # # MaeIII: # [GTNAC] # 24 50 117 162 486 690 # 1120 # # MboI: # [GATC] # 81 114 165 180 303 321 # 666 819 # # MboII: # [GAAGA] # 3 5 91 114 166 371 # 856 1043 # # McrI*: # [CGRYCG] # 573 2076 # # MlyI: # [GAGTC] # 895 1754 # # MmeI: # [TCCRAC] # 355 488 531 1275 # # MnlI: # [CCTC] # 1 3 22 26 50 100 # 130 136 141 142 146 147 # 250 323 1032 # # MseI: # [TTAA] # 6 8 27 38 42 60 # 62 76 78 92 121 140 # 141 148 154 161 189 536 # 570 # # MslI: # [CAYNNNNRTG] # 932 1717 # # MwoI: # [GCNNNNNNNGC] # 11 158 162 192 232 1894 # # NarI: # [GGCGCC] # 154 2495 # # NdeI: # [CATATG] # 792 1857 # # NheI: # [GCTAGC] # 45 616 1988 # # NlaIII: # [CATG] # 11 136 140 148 182 194 # 358 436 495 549 # # NlaIV: # [GGNNCC] # 5 15 24 153 2452 # # NspI: # [RCATGY] # 11 342 436 495 676 689 # # PfoI: # [TCCNGGA] # 1122 1527 # # PleI: # [GAGTC] # 895 1754 # # PpuMI: # [RGGWCCY] # 43 2606 # # PsiI: # [TTATAA] # 545 852 1252 # # RsaI: # [GTAC] # 5 24 42 220 301 2057 # # SacI: # [GAGCTC] # 250 2399 # # SalI: # [GTCGAC] # 165 2484 # # SapI: # [GCTCTTC] # 179 1038 1432 # # ScrFI: # [CCNGG] # 55 180 227 1067 1120 # # SduI: # [GDGCHC] # 250 269 2130 # # SecI*: # [CCNNGG] # 56 57 178 1010 1348 # # SetI: # [ASST] # 4 6 8 9 9 9 # 12 13 15 15 15 16 # 19 21 23 25 25 25 # 28 38 39 45 58 67 # 74 76 78 81 86 101 # 107 144 166 231 244 285 # 432 # # SfaNI: # [GCATC] # 141 201 787 1520 # # SmlI: # [CTYRAG] # 59 603 920 1067 # # SphI: # [GCATGC] # 931 1718 # # SspI: # [AATATT] # 534 624 1491 # # StyI: # [CCWWGG] # 1188 1461 # # TaiI: # [ACGT] # 957 1692 # # TaqI: # [TCGA] # 16 50 52 64 67 92 # 99 137 170 288 315 1299 # # TaqII: # [CACCCA] # 1114 1535 # # TauI: # [GCSGC] # 358 2291 # # TfiI: # [GAWTC] # 57 65 77 84 93 102 # 157 201 243 309 392 869 # # Tsp45I: # [GTSAC] # 50 303 690 1606 # # Tsp4CI*: # [ACNGT] # 88 341 376 506 636 702 # # TspDTI: # [ATGAA] # 48 63 101 166 177 266 # 310 386 452 680 # # TspEI: # [AATT] # 9 13 16 16 27 31 # 32 53 56 64 65 69 # 83 98 109 135 138 158 # 185 537 755 # # TspGWI: # [ACGGA] # 49 184 185 282 1949 # # TspRI: # [CASTGNN] # 519 541 1589 # # TstI: # [CACNNNNNNTCC] # 32 1049 1568 # # VspI: # [ATTAAT] # 536 793 1320 # # XbaI: # [TCTAGA] # 28 800 1821 # # XmaIII*: # [CGGCCG] # 570 2079 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 2649 # Reported_sequences: 1 # Reported_hitcount: 592 #---------------------------------------