Restriction Fragments for SPG1/YGR236C



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The currently selected gene/sequence is :

SPG1/YGR236C on chromosome VII from coordinates 962817 to 962530.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.5314.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 1 5 + TspDTI ATGAA 16 14 . . 10 14 + TfiI GAWTC 10 13 . . 10 14 + HinfI GANTC 10 13 . . 13 18 + Hpy178III* TCNNGA 14 16 . . 24 28 + DdeI CTNAG 24 27 . . 24 28 + BspCNI CTCAG 37 35 . . 24 28 + BseMII CTCAG 38 36 . . 25 29 + Hpy188I TCNGA 27 26 . . 28 31 - MnlI CCTC 21 20 . . 77 80 + CviJI RGCY 78 78 . . 85 90 + HaeII RGCGCY 89 85 . . 86 89 + Hin6I GCGC 86 88 . . 86 89 + GlaI GCGC 87 87 . . 86 89 + HhaI GCGC 88 86 . . 88 92 + BlsI GCNGC 90 89 . . 88 92 + BisI GCNGC 89 90 . . 88 92 + TseI GCWGC 88 91 . . 88 92 - BbvI GCAGC 75 79 . . 89 94 - EcoP15I CAGCAG 61 63 . . 96 101 + KpnI GGTACC 100 96 . . 96 101 + NlaIV GGNNCC 98 98 . . 96 101 + HgiCI* GGYRCC 96 100 . . 96 101 + Acc65I GGTACC 96 100 . . 97 100 + Csp6I GTAC 97 99 . . 97 100 + RsaI GTAC 98 98 . . 101 106 + McrI* CGRYCG 104 102 . . 104 107 + AciI CCGC 104 106 . . 111 114 + FatI CATG 110 114 . . 111 114 + NlaIII CATG 114 110 . . 111 114 + CviAII CATG 111 113 . . 114 119 + BseRI GAGGAG 129 127 . . 114 117 - MnlI CCTC 107 106 . . 121 124 + CviJI RGCY 122 122 . . 119 122 - MnlI CCTC 112 111 . . 127 131 + Tsp4CI* ACNGT 129 128 . . 129 134 + NlaIV GGNNCC 131 131 . . 135 138 + CviRI* TGCA 136 136 . . 134 139 - BsgI GTGCAG 119 117 . . 142 145 + SetI ASST 145 141 . . 142 145 + CviJI RGCY 143 143 . . 142 145 + AluI AGCT 143 143 . . 144 148 + DdeI CTNAG 144 147 . . 158 162 + BceAI ACGGC 174 176 . . 163 168 + Hpy166II GTNNAC 165 165 . . 163 168 + AccI GTMKAC 164 166 . . 173 177 + DdeI CTNAG 173 176 . . 177 182 + AcyI GRCGYC 178 180 . . 178 182 - HgaI GACGC 167 172 . . 191 194 + Csp6I GTAC 191 193 . . 191 194 + RsaI GTAC 192 192 . . 205 209 + BlsI GCNGC 207 206 . . 205 209 + BisI GCNGC 206 207 . . 205 209 + BbvI GCAGC 217 221 . . 205 209 + TseI GCWGC 205 208 . . 207 210 + SetI ASST 210 206 . . 207 210 + CviJI RGCY 208 208 . . 207 210 + AluI AGCT 208 208 . . 216 220 - Hin4II* CCTTC 210 209 . . 227 232 + Hpy166II GTNNAC 229 229 . . 243 247 + FokI GGATG 256 260 . . 243 247 + BseGI GGATG 249 247 . . 240 244 - BccI CCATC 234 235 . . 251 255 + TspDTI ATGAA 266 264 . . 256 260 + MaeIII GTNAC 255 260 . . 259 262 + TaiI ACGT 262 258 . . 259 262 + SetI ASST 262 258 . . 259 262 + MaeII ACGT 259 261 . . 261 273 - PsrI GAACNNNNNNTAC 285 280 253 248 273 278 + Ksp632I* CTCTTC 279 282 . . 274 278 - MboII GAAGA 266 265 . . 282 285 + FatI CATG 281 285 . . 282 285 + NlaIII CATG 285 281 . . 282 285 + CviAII CATG 282 284 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 96 192 # # AccI: # [GTMKAC] # 124 164 # # AciI: # [CCGC] # 104 184 # # AcyI: # [GRCGYC] # 110 178 # # AluI: # [AGCT] # 65 80 143 # # BbvI: # [GCAGC] # 71 75 142 # # BccI: # [CCATC] # 54 234 # # BceAI: # [ACGGC] # 114 174 # # BisI: # [GCNGC] # 82 89 117 # # BlsI: # [GCNGC] # 81 90 117 # # BseGI: # [GGATG] # 39 249 # # BseMII: # [CTCAG] # 38 250 # # BseRI: # [GAGGAG] # 129 159 # # BsgI: # [GTGCAG] # 119 169 # # BspCNI: # [CTCAG] # 37 251 # # Csp6I: # [GTAC] # 94 97 97 # # CviAII: # [CATG] # 6 111 171 # # CviJI: # [RGCY] # 21 44 65 78 80 # # CviRI*: # [TGCA] # 136 152 # # DdeI: # [CTNAG] # 24 29 115 120 # # EcoP15I: # [CAGCAG] # 61 227 # # FatI: # [CATG] # 7 110 171 # # FokI: # [GGATG] # 32 256 # # GlaI: # [GCGC] # 87 201 # # HaeII: # [RGCGCY] # 89 199 # # HgaI: # [GACGC] # 121 167 # # HgiCI*: # [GGYRCC] # 96 192 # # HhaI: # [GCGC] # 88 200 # # Hin4II*: # [CCTTC] # 78 210 # # Hin6I: # [GCGC] # 86 202 # # HinfI: # [GANTC] # 10 278 # # Hpy166II: # [GTNNAC] # 59 64 165 # # Hpy178III*: # [TCNNGA] # 14 274 # # Hpy188I: # [TCNGA] # 27 261 # # KpnI: # [GGTACC] # 100 188 # # Ksp632I*: # [CTCTTC] # 9 279 # # MaeII: # [ACGT] # 29 259 # # MaeIII: # [GTNAC] # 33 255 # # MboII: # [GAAGA] # 22 266 # # McrI*: # [CGRYCG] # 104 184 # # MnlI: # [CCTC] # 5 21 86 176 # # NlaIII: # [CATG] # 3 114 171 # # NlaIV: # [GGNNCC] # 33 98 157 # # PsrI: # [GAACNNNNNNTAC] # 3 32 253 # # RsaI: # [GTAC] # 94 96 98 # # SetI: # [ASST] # 26 52 65 145 # # TaiI: # [ACGT] # 26 262 # # TfiI: # [GAWTC] # 10 278 # # TseI: # [GCWGC] # 83 88 117 # # Tsp4CI*: # [ACNGT] # 129 159 # # TspDTI: # [ATGAA] # 16 22 250 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 288 # Reported_sequences: 1 # Reported_hitcount: 73 #---------------------------------------