Restriction Fragments for THI4/YGR144W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

THI4/YGR144W on chromosome VII from coordinates 780399 to 781379.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.15612.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 13 + SetI ASST 13 9 . . 11 14 + MnlI CCTC 21 20 . . 35 38 + MnlI CCTC 45 44 . . 40 45 + MfeI CAATTG 40 44 . . 41 44 + TspEI AATT 40 44 . . 44 47 + CviRI* TGCA 45 45 . . 49 52 + MseI TTAA 49 51 . . 64 68 + MaeIII GTNAC 63 68 . . 59 64 - Eco57MI CTGRAG 44 42 . . 59 64 - GsuI CTGGAG 44 42 . . 61 65 - BsrI ACTGG 61 59 . . 70 76 + TspRI CASTGNN 76 67 . . 70 75 - BtsI GCAGTG 69 67 . . 79 83 + Hpy188I TCNGA 81 80 . . 89 92 + MseI TTAA 89 91 . . 97 101 + MboII GAAGA 109 108 . . 106 110 + Hpy188I TCNGA 108 107 . . 112 117 + AhaIII* TTTAAA 114 114 . . 113 116 + MseI TTAA 113 115 . . 115 120 + ApoI RAATTY 115 119 . . 116 119 + TspEI AATT 115 119 . . 125 129 + BccI CCATC 133 134 . . 129 132 + AciI CCGC 129 131 . . 126 130 - FokI GGATG 112 116 . . 126 130 - BseGI GGATG 125 123 . . 130 133 + FnuDII* CGCG 131 131 . . 133 137 + TfiI GAWTC 133 136 . . 133 137 + HinfI GANTC 133 136 . . 138 144 + TspRI CASTGNN 144 135 . . 139 143 + Tsp4CI* ACNGT 141 140 . . 142 146 + BsmAI GTCTC 147 151 . . 146 151 - BsiI* CACGAG 146 150 . . 174 179 + XhoII RGATCY 174 178 . . 174 178 + BinI* GGATC 182 183 . . 175 178 + DpnI GATC 176 176 . . 175 178 + BstKTI GATC 177 175 . . 175 178 + MboI GATC 174 178 . . 177 182 + Hpy178III* TCNNGA 178 180 . . 190 194 - BceAI ACGGC 175 177 . . 196 200 + Hpy188I TCNGA 198 197 . . 200 203 + TaiI ACGT 203 199 . . 200 203 + SetI ASST 203 199 . . 200 203 + MaeII ACGT 200 202 . . 199 204 - BtrI CACGTC 201 201 . . 217 222 + Cac8I GCNNGC 219 219 . . 219 224 + SduI GDGCHC 223 219 . . 219 224 + HgiJII* GRGCYC 223 219 . . 220 223 + CviJI RGCY 221 221 . . 217 220 - AciI CCGC 217 219 . . 217 221 - FauI CCCGC 210 212 . . 221 227 + SapI GCTCTTC 228 231 . . 222 227 + Ksp632I* CTCTTC 228 231 . . 223 227 - MboII GAAGA 215 214 . . 228 231 + SetI ASST 231 227 . . 224 229 - Eco57MI CTGRAG 209 207 . . 224 229 - Eco57I CTGAAG 209 207 . . 236 239 + AciI CCGC 236 238 . . 238 242 + TauI GCSGC 241 238 . . 238 242 + BlsI GCNGC 240 239 . . 238 242 + BisI GCNGC 239 240 . . 239 242 + AciI CCGC 239 241 . . 235 240 - EciI GGCGGA 225 223 . . 245 248 + TaiI ACGT 248 244 . . 245 248 + SetI ASST 248 244 . . 245 248 + MaeII ACGT 245 247 . . 249 254 - BtgZI GCGATG 234 238 . . 275 278 + SetI ASST 278 274 . . 273 277 - Hin4II* CCTTC 267 266 . . 287 290 + TaqI TCGA 287 289 . . 300 303 + CviRI* TGCA 301 301 . . 302 310 + DraIII CACNNNGTG 307 304 . . 303 309 + SexAI ACCWGGT 303 308 . . 304 308 + BssKI CCNGG 303 308 . . 304 308 + BseBI CCWGG 305 306 . . 304 308 + ScrFI CCNGG 305 306 . . 304 308 + EcoRII CCWGG 303 308 . . 304 314 + BsiYI* CCNNNNNNNGG 310 307 . . 304 314 + PflMI CCANNNNNTGG 310 307 . . 306 309 + SetI ASST 309 305 . . 323 328 - TaqII CACCCA 313 311 . . 332 335 + TspEI AATT 331 335 . . 344 349 + DsaI* CCRYGG 344 348 . . 344 349 + StyI CCWWGG 344 348 . . 344 349 + NcoI CCATGG 344 348 . . 344 349 + SecI* CCNNGG 344 348 . . 345 354 + MslI CAYNNNNRTG 349 349 . . 345 348 + FatI CATG 344 348 . . 345 348 + NlaIII CATG 348 344 . . 345 348 + CviAII CATG 345 347 . . 347 352 - TsoI TARCCA 337 335 . . 363 366 + SetI ASST 366 362 . . 363 366 + CviJI RGCY 364 364 . . 363 366 + AluI AGCT 364 364 . . 384 389 - MmeI TCCRAC 365 363 . . 400 404 + MboII GAAGA 412 411 . . 400 405 + BbvII* GAAGAC 407 411 . . 408 411 + SetI ASST 411 407 . . 409 413 + HphI GGTGA 421 420 . . 410 414 + Tsp45I GTSAC 409 414 . . 410 414 + MaeIII GTNAC 409 414 . . 406 410 - Hin4II* CCTTC 400 399 . . 412 423 + DrdI GACNNNNNNGTC 418 416 . . 425 428 + MseI TTAA 425 427 . . 429 434 + NspI RCATGY 433 429 . . 429 434 + Cac8I GCNNGC 431 431 . . 429 434 + SphI GCATGC 433 429 . . 430 433 + FatI CATG 429 433 . . 430 433 + NlaIII CATG 433 429 . . 430 433 + CviAII CATG 430 432 . . 433 437 + TauI GCSGC 436 433 . . 433 437 + BlsI GCNGC 435 434 . . 433 437 + BisI GCNGC 434 435 . . 434 437 + AciI CCGC 434 436 . . 442 446 - TspDTI ATGAA 432 430 . . 451 455 + Tsp4CI* ACNGT 453 452 . . 456 466 + EcoNI CCTNNNNNAGG 460 461 . . 456 466 + BsiYI* CCNNNNNNNGG 462 459 . . 464 467 + SetI ASST 467 463 . . 470 473 + CviRI* TGCA 471 471 . . 473 476 + TspEI AATT 472 476 . . 485 488 + MseI TTAA 485 487 . . 489 493 + Tsp4CI* ACNGT 491 490 . . 502 505 + SetI ASST 505 501 . . 511 515 + MboII GAAGA 523 522 . . 519 525 + BstEII GGTNACC 519 524 . . 520 524 + MaeIII GTNAC 519 524 . . 518 523 - TsoI TARCCA 508 506 . . 531 534 + SetI ASST 534 530 . . 550 554 + Tsp45I GTSAC 549 554 . . 550 554 + MaeIII GTNAC 549 554 . . 553 557 + Tsp4CI* ACNGT 555 554 . . 551 555 - HphI GGTGA 543 542 . . 568 572 + Tsp45I GTSAC 567 572 . . 568 572 + MaeIII GTNAC 567 572 . . 569 573 - HphI GGTGA 561 560 . . 575 579 + BsrI ACTGG 580 578 . . 580 583 + TaiI ACGT 583 579 . . 580 583 + SetI ASST 583 579 . . 580 583 + MaeII ACGT 580 582 . . 586 590 + MaeIII GTNAC 585 590 . . 594 597 + SetI ASST 597 593 . . 594 597 + CviJI RGCY 595 595 . . 594 597 + AluI AGCT 595 595 . . 599 603 + Tsp4CI* ACNGT 601 600 . . 602 605 + Csp6I GTAC 602 604 . . 602 605 + RsaI GTAC 603 603 . . 613 616 + CviRI* TGCA 614 614 . . 615 618 + FatI CATG 614 618 . . 615 618 + NlaIII CATG 618 614 . . 615 618 + CviAII CATG 615 617 . . 618 623 + NlaIV GGNNCC 620 620 . . 618 622 + BmgT120I GGNCC 619 620 . . 618 622 + AvaII GGWCC 618 621 . . 618 622 + AsuI* GGNCC 618 621 . . 626 629 + TaiI ACGT 629 625 . . 626 629 + SetI ASST 629 625 . . 626 629 + MaeII ACGT 626 628 . . 630 633 + TspEI AATT 629 633 . . 635 638 + TspEI AATT 634 638 . . 642 645 + SetI ASST 645 641 . . 644 648 + MaeIII GTNAC 643 648 . . 640 645 - BspMI ACCTGC 631 635 . . 656 660 + TspGWI ACGGA 671 669 . . 663 668 + Hpy178III* TCNNGA 664 666 . . 665 669 + Tsp45I GTSAC 664 669 . . 665 669 + MaeIII GTNAC 664 669 . . 666 677 + BdaI TGANNNNNNTCA 655 653 689 687 667 676 + PshAI GACNNNNGTC 671 671 . . 662 667 - BsiI* CACGAG 662 666 . . 669 674 + SmlI CTYRAG 669 673 . . 669 674 + Bce83I* CTTGAG 690 688 . . 672 676 + PleI GAGTC 680 681 . . 672 676 + MlyI GAGTC 681 681 . . 672 676 + HinfI GANTC 672 675 . . 666 677 - BdaI TGANNNNNNTCA 689 687 655 653 682 685 + FatI CATG 681 685 . . 682 685 + NlaIII CATG 685 681 . . 682 685 + CviAII CATG 682 684 . . 703 708 + Cfr10I RCCGGY 703 707 . . 703 708 + BetI* WCCGGW 703 707 . . 703 708 + AgeI ACCGGT 703 707 . . 704 707 + HpaII CCGG 704 706 . . 708 713 + Hpy178III* TCNNGA 709 711 . . 708 713 + BspHI TCATGA 708 712 . . 709 712 + FatI CATG 708 712 . . 709 712 + NlaIII CATG 712 708 . . 709 712 + CviAII CATG 709 711 . . 715 719 + BmgT120I GGNCC 716 717 . . 715 719 + AvaII GGWCC 715 718 . . 715 719 + AsuI* GGNCC 715 718 . . 712 716 - BccI CCATC 706 707 . . 727 737 + MwoI GCNNNNNNNGC 733 730 . . 734 737 + Hin6I GCGC 734 736 . . 734 737 + GlaI GCGC 735 735 . . 734 737 + HhaI GCGC 736 734 . . 743 747 + TfiI GAWTC 743 746 . . 743 747 + HinfI GANTC 743 746 . . 747 751 + Hpy99I CGWCG 751 746 . . 748 753 + SalI GTCGAC 748 752 . . 748 753 + Hpy166II GTNNAC 750 750 . . 748 753 + HindII GTYRAC 750 750 . . 748 753 + AccI GTMKAC 749 751 . . 749 752 + TaqI TCGA 749 751 . . 756 761 + BclI TGATCA 756 760 . . 757 760 + DpnI GATC 758 758 . . 757 760 + BstKTI GATC 759 757 . . 757 760 + MboI GATC 756 760 . . 765 775 + BsiYI* CCNNNNNNNGG 771 768 . . 765 775 + PflMI CCANNNNNTGG 771 768 . . 770 773 + TspEI AATT 769 773 . . 776 779 - AciI CCGC 776 778 . . 781 785 + TspDTI ATGAA 796 794 . . 783 787 - Hin4II* CCTTC 777 776 . . 789 794 + Hpy178III* TCNNGA 790 792 . . 795 798 + FatI CATG 794 798 . . 795 798 + NlaIII CATG 798 794 . . 795 798 + CviAII CATG 795 797 . . 796 800 + TspDTI ATGAA 811 809 . . 802 805 + FatI CATG 801 805 . . 802 805 + NlaIII CATG 805 801 . . 802 805 + CviAII CATG 802 804 . . 826 835 + MslI CAYNNNNRTG 830 830 . . 826 835 + OliI CACNNNNGTG 830 830 . . 834 837 + CviRI* TGCA 835 835 . . 840 847 + SgrAI CRCCGGYG 841 845 . . 841 846 + Cfr10I RCCGGY 841 845 . . 842 845 + HpaII CCGG 842 844 . . 847 852 + Hpy166II GTNNAC 849 849 . . 847 852 + HindII GTYRAC 849 849 . . 854 859 + BspLU11I* ACATGT 854 858 . . 854 859 + NspI RCATGY 858 854 . . 854 859 + AflIII ACRYGT 854 858 . . 855 858 + FatI CATG 854 858 . . 855 858 + NlaIII CATG 858 854 . . 855 858 + CviAII CATG 855 857 . . 857 862 + TatI WGTACW 857 861 . . 858 861 + Csp6I GTAC 858 860 . . 858 861 + RsaI GTAC 859 859 . . 885 889 + BsrI ACTGG 890 888 . . 888 892 + FokI GGATG 901 905 . . 888 892 + BseGI GGATG 894 892 . . 889 893 - BccI CCATC 883 884 . . 895 898 + MseI TTAA 895 897 . . 899 903 + Tsp4CI* ACNGT 901 900 . . 906 911 + NlaIV GGNNCC 908 908 . . 907 911 + BmgT120I GGNCC 908 909 . . 907 911 + AvaII GGWCC 907 910 . . 907 911 + AsuI* GGNCC 907 910 . . 909 914 + MmeI TCCRAC 934 932 . . 910 920 + BsiYI* CCNNNNNNNGG 916 913 . . 910 920 + PflMI CCANNNNNTGG 916 913 . . 921 924 + SetI ASST 924 920 . . 921 924 + CviJI RGCY 922 922 . . 921 924 + AluI AGCT 922 922 . . 927 930 + CviJI RGCY 928 928 . . 943 946 + FatI CATG 942 946 . . 943 946 + NlaIII CATG 946 942 . . 943 946 + CviAII CATG 943 945 . . 941 945 - TspDTI ATGAA 931 929 . . 946 950 + BlsI GCNGC 948 947 . . 946 950 + BisI GCNGC 947 948 . . 946 950 + TseI GCWGC 946 949 . . 949 955 + EspI* GCTNAGC 950 953 . . 950 954 + DdeI CTNAG 950 953 . . 946 950 - BbvI GCAGC 933 937 . . 947 952 - EcoP15I CAGCAG 919 921 . . 950 954 - BspCNI CTCAG 942 940 . . 950 954 - BseMII CTCAG 941 939 . . 956 961 + ApoI RAATTY 956 960 . . 957 960 + TspEI AATT 956 960 . . 973 977 + BlsI GCNGC 975 974 . . 973 977 + BisI GCNGC 974 975 . . 973 977 + TseI GCWGC 973 976 . . 977 981 + DdeI CTNAG 977 980 . . 973 977 - BbvI GCAGC 960 964 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 232 749 # # AciI: # [CCGC] # 3 19 88 129 195 205 # 342 # # AflIII: # [ACRYGT] # 127 854 # # AgeI: # [ACCGGT] # 278 703 # # AhaIII*: # [TTTAAA] # 114 867 # # AluI: # [AGCT] # 59 231 327 364 # # ApoI: # [RAATTY] # 25 115 841 # # AsuI*: # [GGNCC] # 74 97 192 618 # # AvaII: # [GGWCC] # 74 97 192 618 # # BbvI: # [GCAGC] # 21 27 933 # # BbvII*: # [GAAGAC] # 407 574 # # BccI: # [CCATC] # 98 133 177 573 # # Bce83I*: # [CTTGAG] # 291 690 # # BceAI: # [ACGGC] # 175 806 # # BclI: # [TGATCA] # 225 756 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 292 655 # # BetI*: # [WCCGGW] # 278 703 # # BinI*: # [GGATC] # 182 799 # # BisI: # [GCNGC] # 7 27 195 239 513 # # BlsI: # [GCNGC] # 6 27 195 240 513 # # BmgT120I: # [GGNCC] # 73 97 192 619 # # BseBI: # [CCWGG] # 305 676 # # BseGI: # [GGATG] # 87 125 769 # # BseMII: # [CTCAG] # 40 941 # # BsiI*: # [CACGAG] # 146 319 516 # # BsiYI*: # [CCNNNNNNNGG] # 65 145 152 309 310 # # BsmAI: # [GTCTC] # 147 834 # # BspCNI: # [CTCAG] # 39 942 # # BspHI: # [TCATGA] # 273 708 # # BspLU11I*: # [ACATGT] # 127 854 # # BspMI: # [ACCTGC] # 350 631 # # BsrI: # [ACTGG] # 61 91 310 519 # # BssKI: # [CCNGG] # 303 678 # # BstEII: # [GGTNACC] # 462 519 # # BstKTI: # [GATC] # 177 222 582 # # BtgZI: # [GCGATG] # 234 747 # # BtrI: # [CACGTC] # 201 780 # # BtsI: # [GCAGTG] # 69 912 # # Cac8I: # [GCNNGC] # 212 219 550 # # Cfr10I: # [RCCGGY] # 138 140 703 # # Csp6I: # [GTAC] # 123 256 602 # # CviAII: # [CATG] # 7 27 38 53 67 85 # 86 88 185 345 # # CviJI: # [RGCY] # 6 53 143 221 231 327 # # CviRI*: # [TGCA] # 45 143 146 170 221 256 # # DdeI: # [CTNAG] # 4 27 950 # # DpnI: # [GATC] # 176 223 582 # # DraIII: # [CACNNNGTG] # 307 674 # # DrdI: # [GACNNNNNNGTC] # 418 563 # # DsaI*: # [CCRYGG] # 344 637 # # EciI: # [GGCGGA] # 225 756 # # Eco57I: # [CTGAAG] # 209 772 # # Eco57MI: # [CTGRAG] # 44 165 772 # # EcoNI: # [CCTNNNNNAGG] # 460 521 # # EcoP15I: # [CAGCAG] # 62 919 # # EcoRII: # [CCWGG] # 303 678 # # EspI*: # [GCTNAGC] # 31 950 # # FatI: # [CATG] # 7 27 39 53 67 85 # 86 88 185 344 # # FauI: # [CCCGC] # 210 771 # # FnuDII*: # [CGCG] # 131 850 # # FokI: # [GGATG] # 80 112 789 # # GlaI: # [GCGC] # 246 735 # # GsuI: # [CTGGAG] # 44 937 # # HgiJII*: # [GRGCYC] # 223 758 # # HhaI: # [GCGC] # 245 736 # # Hin4II*: # [CCTTC] # 133 204 267 377 # # Hin6I: # [GCGC] # 247 734 # # HindII: # [GTYRAC] # 99 132 750 # # HinfI: # [GANTC] # 71 133 238 539 # # HpaII: # [CCGG] # 138 139 704 # # HphI: # [GGTGA] # 18 122 420 421 # # Hpy166II: # [GTNNAC] # 99 132 750 # # Hpy178III*: # [TCNNGA] # 45 81 178 191 486 # # Hpy188I: # [TCNGA] # 27 81 90 783 # # Hpy99I: # [CGWCG] # 230 751 # # Ksp632I*: # [CTCTTC] # 228 753 # # MaeII: # [ACGT] # 45 46 200 335 355 # # MaeIII: # [GTNAC] # 18 18 21 30 58 63 # 110 317 346 # # MboI: # [GATC] # 174 225 582 # # MboII: # [GAAGA] # 106 109 111 197 458 # # MfeI: # [CAATTG] # 40 941 # # MlyI: # [GAGTC] # 300 681 # # MmeI: # [TCCRAC] # 47 365 569 # # MnlI: # [CCTC] # 21 24 936 # # MseI: # [TTAA] # 24 40 49 60 86 312 # 410 # # MslI: # [CAYNNNNRTG] # 151 349 481 # # MwoI: # [GCNNNNNNNGC] # 248 733 # # NcoI: # [CCATGG] # 344 637 # # NlaIII: # [CATG] # 7 27 35 53 67 85 # 86 88 185 348 # # NlaIV: # [GGNNCC] # 73 288 620 # # NspI: # [RCATGY] # 123 425 433 # # OliI: # [CACNNNNGTG] # 151 830 # # PflMI: # [CCANNNNNTGG] # 65 145 310 461 # # PleI: # [GAGTC] # 301 680 # # PshAI: # [GACNNNNGTC] # 310 671 # # RsaI: # [GTAC] # 122 256 603 # # SalI: # [GTCGAC] # 233 748 # # SapI: # [GCTCTTC] # 228 753 # # ScrFI: # [CCNGG] # 305 676 # # SduI: # [GDGCHC] # 223 758 # # SecI*: # [CCNNGG] # 344 637 # # SetI: # [ASST] # 13 14 16 17 28 29 # 30 31 32 38 45 49 # 56 57 57 190 279 # # SexAI: # [ACCWGGT] # 303 678 # # SgrAI: # [CRCCGGYG] # 140 841 # # SmlI: # [CTYRAG] # 312 669 # # SphI: # [GCATGC] # 433 548 # # StyI: # [CCWWGG] # 344 637 # # TaiI: # [ACGT] # 45 46 203 335 352 # # TaqI: # [TCGA] # 232 287 462 # # TaqII: # [CACCCA] # 313 668 # # TatI: # [WGTACW] # 124 857 # # TauI: # [GCSGC] # 195 241 545 # # TfiI: # [GAWTC] # 133 238 610 # # TseI: # [GCWGC] # 8 27 946 # # TsoI: # [TARCCA] # 171 337 473 # # Tsp45I: # [GTSAC] # 18 97 140 317 409 # # Tsp4CI*: # [ACNGT] # 38 46 64 80 141 300 # 312 # # TspDTI: # [ATGAA] # 15 50 120 364 432 # # TspEI: # [AATT] # 5 25 40 75 135 141 # 157 187 216 # # TspGWI: # [ACGGA] # 310 671 # # TspRI: # [CASTGNN] # 68 76 837 # # XhoII: # [RGATCY] # 174 807 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 981 # Reported_sequences: 1 # Reported_hitcount: 275 #---------------------------------------