Restriction Fragments for RPS23A/YGR118W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

RPS23A/YGR118W on chromosome VII from coordinates 726974 to 727730.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.20193.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 12 + SetI ASST 12 8 . . 14 17 + CviJI RGCY 15 15 . . 21 24 + SetI ASST 24 20 . . 20 23 - MnlI CCTC 13 12 . . 35 38 + MaeI CTAG 35 37 . . 41 44 + SetI ASST 44 40 . . 41 44 + CviJI RGCY 42 42 . . 41 44 + AluI AGCT 42 42 . . 44 49 + BsaAI YACGTR 46 46 . . 45 48 + TaiI ACGT 48 44 . . 45 48 + SetI ASST 48 44 . . 45 48 + MaeII ACGT 45 47 . . 45 50 + AflIII ACRYGT 45 49 . . 49 54 + Hpy166II GTNNAC 51 51 . . 56 60 + MboII GAAGA 68 67 . . 70 75 + Hpy166II GTNNAC 72 72 . . 70 75 + HindII GTYRAC 72 72 . . 84 87 + TspEI AATT 83 87 . . 86 89 + MseI TTAA 86 88 . . 114 119 + EcoRV GATATC 116 116 . . 125 128 + SetI ASST 128 124 . . 126 130 + HphI GGTGA 138 137 . . 129 133 - Hin4II* CCTTC 123 122 . . 132 143 - TstI CACNNNNNNTCC 156 151 124 119 143 148 + SacI GAGCTC 147 143 . . 143 148 + Ecl136II GAGCTC 145 145 . . 143 148 + SduI GDGCHC 147 143 . . 143 148 + HgiJII* GRGCYC 147 143 . . 143 148 + HgiAI* GWGCWC 147 143 . . 144 147 + SetI ASST 147 143 . . 144 147 + CviJI RGCY 145 145 . . 144 147 + AluI AGCT 145 145 . . 147 152 + Hpy178III* TCNNGA 148 150 . . 148 153 + Eco57MI CTGRAG 169 167 . . 148 153 + GsuI CTGGAG 169 167 . . 158 163 + ApoI RAATTY 158 162 . . 159 162 + TspEI AATT 158 162 . . 168 172 + MaeIII GTNAC 167 172 . . 177 181 + BssKI CCNGG 176 181 . . 177 181 + BseBI CCWGG 178 179 . . 177 181 + ScrFI CCNGG 178 179 . . 177 181 + EcoRII CCWGG 176 181 . . 181 184 + Hin6I GCGC 181 183 . . 181 184 + GlaI GCGC 182 182 . . 181 184 + HhaI GCGC 183 181 . . 200 203 + CviJI RGCY 201 201 . . 198 201 - MnlI CCTC 191 190 . . 203 206 + MseI TTAA 203 205 . . 211 216 + AflIII ACRYGT 211 215 . . 212 217 + BtrI CACGTC 214 214 . . 213 216 + TaiI ACGT 216 212 . . 213 216 + SetI ASST 216 212 . . 213 216 + MaeII ACGT 213 215 . . 215 219 + Tsp45I GTSAC 214 219 . . 215 219 + MaeIII GTNAC 214 219 . . 218 222 + Tsp4CI* ACNGT 220 219 . . 219 225 + TspRI CASTGNN 225 216 . . 234 238 + TspDTI ATGAA 249 247 . . 241 246 + Hpy166II GTNNAC 243 243 . . 245 249 + Tsp4CI* ACNGT 247 246 . . 251 254 + SetI ASST 254 250 . . 251 254 + CviJI RGCY 252 252 . . 251 254 + AluI AGCT 252 252 . . 256 261 + CfrI YGGCCR 256 260 . . 257 260 + CviJI RGCY 258 258 . . 257 260 + HaeIII GGCC 258 258 . . 263 267 - MboII GAAGA 255 254 . . 273 278 + SfeI* CTRYAG 273 277 . . 291 294 + SetI ASST 294 290 . . 297 301 - MboII GAAGA 289 288 . . 311 315 + DdeI CTNAG 311 314 . . 311 315 + BspCNI CTCAG 324 322 . . 311 315 + BseMII CTCAG 325 323 . . 312 316 + Hpy188I TCNGA 314 313 . . 319 323 + Tsp45I GTSAC 318 323 . . 319 323 + MaeIII GTNAC 318 323 . . 328 331 + CviJI RGCY 329 329 . . 328 331 + HaeIII GGCC 329 329 . . 326 329 - MnlI CCTC 319 318 . . 329 333 - BceAI ACGGC 314 316 . . 342 346 + TspGWI ACGGA 357 355 . . 345 349 + TfiI GAWTC 345 348 . . 345 349 + HinfI GANTC 345 348 . . 362 366 + Tsp4CI* ACNGT 364 363 . . 383 386 + CviJI RGCY 384 384 . . 384 388 - BceAI ACGGC 369 371 . . 390 394 + BmgT120I GGNCC 391 392 . . 390 394 + AsuI* GGNCC 390 393 . . 391 394 + CviJI RGCY 392 392 . . 391 394 + HaeIII GGCC 392 392 . . 409 413 + MboII GAAGA 421 420 . . 409 422 + AjuI GAANNNNNNNTTGG 401 396 433 428 409 414 - Ksp632I* CTCTTC 404 407 . . 423 426 + Csp6I GTAC 423 425 . . 423 426 + RsaI GTAC 424 424 . . 429 433 + Hin4II* CCTTC 439 438 . . 437 441 - MboII GAAGA 429 428 . . 436 441 - BbvII* GAAGAC 429 433 . . 443 453 + BsiYI* CCNNNNNNNGG 449 446 . . 443 453 + PflMI CCANNNNNTGG 449 446 . . 455 459 - MboII GAAGA 447 446 . . 461 464 + FatI CATG 460 464 . . 461 464 + NlaIII CATG 464 460 . . 461 464 + CviAII CATG 461 463 . . 465 470 + StyI CCWWGG 465 469 . . 465 470 + SecI* CCNNGG 465 469 . . 486 489 + TspEI AATT 485 489 . . 495 498 + TaqI TCGA 495 497 . . 497 501 + TfiI GAWTC 497 500 . . 497 501 + HinfI GANTC 497 500 . . 508 511 + SetI ASST 511 507 . . 522 526 + Hpy188I TCNGA 524 523 . . 543 546 + TspEI AATT 542 546 . . 544 549 + VspI ATTAAT 545 547 . . 545 548 + MseI TTAA 545 547 . . 549 554 + Hpy178III* TCNNGA 550 552 . . 555 559 + Tsp4CI* ACNGT 557 556 . . 564 567 + SetI ASST 567 563 . . 566 570 + Tsp45I GTSAC 565 570 . . 566 570 + MaeIII GTNAC 565 570 . . 562 566 - Hin4II* CCTTC 556 555 . . 568 574 + TspRI CASTGNN 574 565 . . 568 573 - BtsI GCAGTG 567 565 . . 581 591 + BsiYI* CCNNNNNNNGG 587 584 . . 581 591 + PflMI CCANNNNNTGG 587 584 . . 587 591 - BccI CCATC 581 582 . . 605 610 + SalI GTCGAC 605 609 . . 605 610 + Hpy166II GTNNAC 607 607 . . 605 610 + HindII GTYRAC 607 607 . . 605 610 + AccI GTMKAC 606 608 . . 606 609 + TaqI TCGA 606 608 . . 607 611 + Hpy99I CGWCG 611 606 . . 618 622 + TspDTI ATGAA 633 631 . . 628 633 + Cac8I GCNNGC 630 630 . . 628 633 + BmtI GCTAGC 632 628 . . 628 633 + NheI GCTAGC 628 632 . . 629 632 + MaeI CTAG 629 631 . . 634 637 + SetI ASST 637 633 . . 632 637 - BspMI ACCTGC 623 627 . . 655 658 + SetI ASST 658 654 . . 655 658 + CviJI RGCY 656 656 . . 655 658 + AluI AGCT 656 656 . . 657 661 + DdeI CTNAG 657 660 . . 656 662 - Bpu10I CCTNAGC 657 660 . . 662 666 + HphI GGTGA 674 673 . . 671 675 + BssKI CCNGG 670 675 . . 671 675 + BseBI CCWGG 672 673 . . 671 675 + ScrFI CCNGG 672 673 . . 671 675 + EcoRII CCWGG 670 675 . . 673 676 + SetI ASST 676 672 . . 681 685 + TfiI GAWTC 681 684 . . 681 685 + HinfI GANTC 681 684 . . 681 691 + Hin4I GAYNNNNNVTC 672 667 704 699 687 690 + SetI ASST 690 686 . . 681 691 - Hin4I GAYNNNNNVTC 704 699 672 667 693 696 + MseI TTAA 693 695 . . 696 699 + SetI ASST 699 695 . . 697 702 + Eco31I GGTCTC 703 707 . . 698 702 + BsmAI GTCTC 703 707 . . 707 711 + BsmAI GTCTC 712 716 . . 718 721 + CviJI RGCY 719 719 . . 736 740 - Hin4II* CCTTC 730 729 . . 744 747 + CviJI RGCY 745 745 . . 750 753 + DpnI GATC 751 751 . . 750 753 + BstKTI GATC 752 750 . . 750 753 + MboI GATC 749 753 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 151 606 # # AflIII: # [ACRYGT] # 45 166 546 # # AjuI: # [GAANNNNNNNTTGG] # 32 324 401 # # AluI: # [AGCT] # 42 101 103 107 404 # # ApoI: # [RAATTY] # 158 599 # # AsuI*: # [GGNCC] # 367 390 # # BbvII*: # [GAAGAC] # 328 429 # # BccI: # [CCATC] # 176 581 # # BceAI: # [ACGGC] # 55 314 388 # # BmgT120I: # [GGNCC] # 366 391 # # BmtI: # [GCTAGC] # 125 632 # # Bpu10I: # [CCTNAGC] # 100 657 # # BsaAI: # [YACGTR] # 46 711 # # BseBI: # [CCWGG] # 85 178 494 # # BseMII: # [CTCAG] # 325 432 # # BsiYI*: # [CCNNNNNNNGG] # 138 170 449 # # BsmAI: # [GTCTC] # 9 45 703 # # BspCNI: # [CTCAG] # 324 433 # # BspMI: # [ACCTGC] # 134 623 # # BssKI: # [CCNGG] # 87 176 494 # # BstKTI: # [GATC] # 5 752 # # BtrI: # [CACGTC] # 214 543 # # BtsI: # [GCAGTG] # 190 567 # # Cac8I: # [GCNNGC] # 127 630 # # CfrI: # [YGGCCR] # 256 501 # # Csp6I: # [GTAC] # 334 423 # # CviAII: # [CATG] # 296 461 # # CviJI: # [RGCY] # 6 8 12 15 26 27 # 51 55 56 63 71 103 # 264 # # DdeI: # [CTNAG] # 100 311 346 # # DpnI: # [GATC] # 6 751 # # Ecl136II: # [GAGCTC] # 145 612 # # Eco31I: # [GGTCTC] # 54 703 # # Eco57MI: # [CTGRAG] # 169 588 # # EcoRII: # [CCWGG] # 87 176 494 # # EcoRV: # [GATATC] # 116 641 # # FatI: # [CATG] # 297 460 # # GlaI: # [GCGC] # 182 575 # # GsuI: # [CTGGAG] # 169 588 # # HaeIII: # [GGCC] # 63 71 258 365 # # HgiAI*: # [GWGCWC] # 147 610 # # HgiJII*: # [GRGCYC] # 147 610 # # HhaI: # [GCGC] # 183 574 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 53 672 # # Hin4II*: # [CCTTC] # 27 117 123 174 316 # # Hin6I: # [GCGC] # 181 576 # # HindII: # [GTYRAC] # 72 150 535 # # HinfI: # [GANTC] # 76 152 184 345 # # HphI: # [GGTGA] # 83 138 536 # # Hpy166II: # [GTNNAC] # 21 51 150 171 364 # # Hpy178III*: # [TCNNGA] # 148 207 402 # # Hpy188I: # [TCNGA] # 210 233 314 # # Hpy99I: # [CGWCG] # 146 611 # # Ksp632I*: # [CTCTTC] # 353 404 # # MaeI: # [CTAG] # 35 128 594 # # MaeII: # [ACGT] # 45 168 544 # # MaeIII: # [GTNAC] # 47 104 167 192 247 # # MboI: # [GATC] # 8 749 # # MboII: # [GAAGA] # 8 18 34 68 132 187 # 310 # # MnlI: # [CCTC] # 13 128 178 438 # # MseI: # [TTAA] # 64 86 117 148 342 # # NheI: # [GCTAGC] # 129 628 # # NlaIII: # [CATG] # 293 464 # # PflMI: # [CCANNNNNTGG] # 138 170 449 # # RsaI: # [GTAC] # 333 424 # # SacI: # [GAGCTC] # 147 610 # # SalI: # [GTCGAC] # 152 605 # # ScrFI: # [CCNGG] # 85 178 494 # # SduI: # [GDGCHC] # 147 610 # # SecI*: # [CCNNGG] # 292 465 # # SetI: # [ASST] # 4 9 12 12 14 18 # 19 20 21 38 40 56 # 58 69 70 80 217 # # SfeI*: # [CTRYAG] # 273 484 # # StyI: # [CCWWGG] # 292 465 # # TaiI: # [ACGT] # 48 168 541 # # TaqI: # [TCGA] # 111 151 495 # # TfiI: # [GAWTC] # 76 152 184 345 # # Tsp45I: # [GTSAC] # 104 192 214 247 # # Tsp4CI*: # [ACNGT] # 27 117 193 200 220 # # TspDTI: # [ATGAA] # 124 249 384 # # TspEI: # [AATT] # 57 75 83 215 327 # # TspGWI: # [ACGGA] # 357 400 # # TspRI: # [CASTGNN] # 183 225 349 # # TstI: # [CACNNNNNNTCC] # 32 124 601 # # VspI: # [ATTAAT] # 212 545 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 757 # Reported_sequences: 1 # Reported_hitcount: 166 #---------------------------------------