Restriction Fragments for YGR109W-B



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The currently selected gene/sequence is :

YGR109W-B on chromosome VII from coordinates 707610 to 712254.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.9389.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 7 + SetI ASST 7 3 . . 4 7 + CviJI RGCY 5 5 . . 4 7 + AluI AGCT 5 5 . . 12 16 + BinI* GGATC 20 21 . . 13 16 + DpnI GATC 14 14 . . 13 16 + BstKTI GATC 15 13 . . 13 16 + MboI GATC 12 16 . . 21 26 + SecI* CCNNGG 21 25 . . 22 26 + BssKI CCNGG 21 26 . . 22 26 + BseBI CCWGG 23 24 . . 22 26 + ScrFI CCNGG 23 24 . . 22 26 + EcoRII CCWGG 21 26 . . 22 32 + BsiYI* CCNNNNNNNGG 28 25 . . 26 31 + BseRI GAGGAG 41 39 . . 26 29 - MnlI CCTC 19 18 . . 29 32 - MnlI CCTC 22 21 . . 34 37 + TspEI AATT 33 37 . . 49 53 - BsrI ACTGG 49 47 . . 48 53 - BfiI ACTGGG 43 42 . . 55 60 + BsmI GAATGC 61 59 . . 55 64 + XmnI GAANNNNTTC 59 59 . . 60 64 + Hin4II* CCTTC 70 69 . . 52 64 - BarI GAAGNNNNNNTAC 76 71 44 39 71 76 + Hpy178III* TCNNGA 72 74 . . 75 78 + DpnI GATC 76 76 . . 75 78 + BstKTI GATC 77 75 . . 75 78 + MboI GATC 74 78 . . 77 82 + MmeI TCCRAC 102 100 . . 75 79 - BinI* GGATC 69 70 . . 82 86 + BccI CCATC 90 91 . . 91 94 + SetI ASST 94 90 . . 92 96 + Hin4II* CCTTC 102 101 . . 95 99 + Hpy188I TCNGA 97 96 . . 93 98 - Eco57MI CTGRAG 78 76 . . 93 98 - Eco57I CTGAAG 78 76 . . 99 102 + SetI ASST 102 98 . . 98 101 - MnlI CCTC 91 90 . . 109 113 + HinfI GANTC 109 112 . . 109 113 - PleI GAGTC 103 104 . . 109 113 - MlyI GAGTC 103 103 . . 128 138 + BsaXI ACNNNNNCTCC 118 115 148 145 136 140 + Hpy188I TCNGA 138 137 . . 148 151 + MseI TTAA 148 150 . . 152 157 + BspLU11I* ACATGT 152 156 . . 152 157 + NspI RCATGY 156 152 . . 152 157 + AflIII ACRYGT 152 156 . . 153 162 + MslI CAYNNNNRTG 157 157 . . 153 156 + FatI CATG 152 156 . . 153 156 + NlaIII CATG 156 152 . . 153 156 + CviAII CATG 153 155 . . 162 167 + EcoRV GATATC 164 164 . . 169 174 + CfrI YGGCCR 169 173 . . 170 173 + CviJI RGCY 171 171 . . 170 173 + HaeIII GGCC 171 171 . . 178 182 - SfaNI GCATC 168 172 . . 182 186 - BsrI ACTGG 182 180 . . 193 198 + Hpy166II GTNNAC 195 195 . . 194 199 + TatI WGTACW 194 198 . . 195 198 + Csp6I GTAC 195 197 . . 195 198 + RsaI GTAC 196 196 . . 198 203 + PstI CTGCAG 202 198 . . 198 203 + SfeI* CTRYAG 198 202 . . 199 202 + CviRI* TGCA 200 200 . . 203 207 + MboII GAAGA 215 214 . . 203 208 + BbvII* GAAGAC 210 214 . . 214 217 + MseI TTAA 214 216 . . 220 224 + FauI CCCGC 228 230 . . 221 226 + NspBII* CMGCKG 223 223 . . 221 224 + AciI CCGC 221 223 . . 223 227 + BlsI GCNGC 225 224 . . 223 227 + BisI GCNGC 224 225 . . 223 227 + TseI GCWGC 223 226 . . 226 236 + MwoI GCNNNNNNNGC 232 229 . . 227 231 + DdeI CTNAG 227 230 . . 227 231 + BspCNI CTCAG 240 238 . . 227 231 + BseMII CTCAG 241 239 . . 223 227 - BbvI GCAGC 210 214 . . 229 235 + TspRI CASTGNN 235 226 . . 234 237 + CviJI RGCY 235 235 . . 246 251 + TatI WGTACW 246 250 . . 246 251 + Bsp1407I TGTACA 246 250 . . 247 250 + Csp6I GTAC 247 249 . . 247 250 + RsaI GTAC 248 248 . . 253 265 + PsrI GAACNNNNNNTAC 245 240 277 272 257 267 + FalI AAGNNNNNCTT 248 243 280 275 258 261 + SetI ASST 261 257 . . 260 265 + Hpy166II GTNNAC 262 262 . . 260 265 + BssNAI GTATAC 262 262 . . 260 265 + AccI GTMKAC 261 263 . . 257 267 - FalI AAGNNNNNCTT 280 275 248 243 278 281 + TaqI TCGA 278 280 . . 286 290 - TspDTI ATGAA 276 274 . . 317 320 + CviJI RGCY 318 318 . . 325 330 + EcoRV GATATC 327 327 . . 341 344 + MseI TTAA 341 343 . . 345 348 + CviRI* TGCA 346 346 . . 361 365 + Hpy188I TCNGA 363 362 . . 364 377 + AjuI GAANNNNNNNTTGG 356 351 388 383 365 375 + FalI AAGNNNNNCTT 356 351 388 383 366 369 + SetI ASST 369 365 . . 366 369 + CviJI RGCY 367 367 . . 366 369 + AluI AGCT 367 367 . . 365 375 - FalI AAGNNNNNCTT 388 383 356 351 385 390 + Esp3I CGTCTC 391 395 . . 386 390 + BsmAI GTCTC 391 395 . . 384 388 - HgaI GACGC 373 378 . . 398 402 + TfiI GAWTC 398 401 . . 398 402 + HinfI GANTC 398 401 . . 401 405 + Hpy188I TCNGA 403 402 . . 413 417 + BslFI GGGAC 427 431 . . 419 424 + BsmI GAATGC 425 423 . . 434 439 + Hpy178III* TCNNGA 435 437 . . 434 439 + BspHI TCATGA 434 438 . . 435 438 + FatI CATG 434 438 . . 435 438 + NlaIII CATG 438 434 . . 435 438 + CviAII CATG 435 437 . . 433 437 - TspDTI ATGAA 423 421 . . 438 443 + TaqII GACCGA 454 452 . . 441 452 + BcgI CGANNNNNNTGC 430 428 464 462 447 450 + SetI ASST 450 446 . . 447 450 + CviJI RGCY 448 448 . . 447 450 + AluI AGCT 448 448 . . 448 452 + BlsI GCNGC 450 449 . . 448 452 + BisI GCNGC 449 450 . . 448 452 + TseI GCWGC 448 451 . . 448 452 - BbvI GCAGC 435 439 . . 467 470 + MaeI CTAG 467 469 . . 470 473 + CviJI RGCY 471 471 . . 487 490 + SetI ASST 490 486 . . 493 498 + SspI AATATT 495 495 . . 500 504 + Hpy188I TCNGA 502 501 . . 503 508 + BsmI GAATGC 509 507 . . 506 509 + CviRI* TGCA 507 507 . . 517 521 - BsmAI GTCTC 511 515 . . 523 526 + MseI TTAA 523 525 . . 529 533 + HgaI GACGC 538 543 . . 529 534 + AcyI GRCGYC 530 532 . . 533 538 + DsaI* CCRYGG 533 537 . . 533 538 + StyI CCWWGG 533 537 . . 533 538 + NcoI CCATGG 533 537 . . 533 538 + SecI* CCNNGG 533 537 . . 534 537 + FatI CATG 533 537 . . 534 537 + NlaIII CATG 537 533 . . 534 537 + CviAII CATG 534 536 . . 538 542 + MboII GAAGA 550 549 . . 543 546 + CviJI RGCY 544 544 . . 538 543 - Ksp632I* CTCTTC 533 536 . . 541 544 - MnlI CCTC 534 533 . . 558 561 + CviRI* TGCA 559 559 . . 557 562 - BsgI GTGCAG 542 540 . . 572 576 + TfiI GAWTC 572 575 . . 572 576 + HinfI GANTC 572 575 . . 575 579 - MboII GAAGA 567 566 . . 579 584 + SecI* CCNNGG 579 583 . . 580 584 + BssKI CCNGG 579 584 . . 580 584 + BseBI CCWGG 581 582 . . 580 584 + ScrFI CCNGG 581 582 . . 580 584 + EcoRII CCWGG 579 584 . . 584 588 + TfiI GAWTC 584 587 . . 584 588 + HinfI GANTC 584 587 . . 586 591 + AsuII TTCGAA 587 589 . . 587 590 + TaqI TCGA 587 589 . . 588 599 + BcgI CGANNNNNNTGC 577 575 611 609 595 599 - SfaNI GCATC 585 589 . . 601 605 - BccI CCATC 595 596 . . 605 609 - BsmAI GTCTC 599 603 . . 605 615 - Hin4I GAYNNNNNVTC 628 623 596 591 619 624 + NlaIV GGNNCC 621 621 . . 619 624 + HgiCI* GGYRCC 619 623 . . 640 644 + MboII GAAGA 652 651 . . 649 653 + HinfI GANTC 649 652 . . 652 656 + Hpy188I TCNGA 654 653 . . 649 653 - PleI GAGTC 643 644 . . 649 653 - MlyI GAGTC 643 643 . . 661 665 + TfiI GAWTC 661 664 . . 661 665 + HinfI GANTC 661 664 . . 664 668 + Hpy188I TCNGA 666 665 . . 667 671 + MboII GAAGA 679 678 . . 675 679 + Hpy188I TCNGA 677 676 . . 692 695 + TaiI ACGT 695 691 . . 692 695 + SetI ASST 695 691 . . 692 695 + MaeII ACGT 692 694 . . 688 693 - BciVI GTATCC 682 681 . . 700 704 + Tsp4CI* ACNGT 702 701 . . 711 721 + FalI AAGNNNNNCTT 702 697 734 729 713 717 + MboII GAAGA 725 724 . . 715 720 + XhoII RGATCY 715 719 . . 715 720 + BglII AGATCT 715 719 . . 716 719 + DpnI GATC 717 717 . . 716 719 + BstKTI GATC 718 716 . . 716 719 + MboI GATC 715 719 . . 711 721 - FalI AAGNNNNNCTT 734 729 702 697 744 747 + TaqI TCGA 744 746 . . 766 770 + BccI CCATC 774 775 . . 769 774 + XbaI TCTAGA 769 773 . . 769 774 + Hpy178III* TCNNGA 770 772 . . 770 773 + MaeI CTAG 770 772 . . 775 779 + MboII GAAGA 787 786 . . 794 797 + CviJI RGCY 795 795 . . 819 823 + BslFI GGGAC 833 837 . . 817 820 - MnlI CCTC 810 809 . . 823 828 - BtgZI GCGATG 808 812 . . 842 847 + SduI GDGCHC 846 842 . . 842 847 + HgiAI* GWGCWC 846 842 . . 844 854 + MwoI GCNNNNNNNGC 850 847 . . 845 850 + BsaAI YACGTR 847 847 . . 846 849 + TaiI ACGT 849 845 . . 846 849 + SetI ASST 849 845 . . 846 849 + MaeII ACGT 846 848 . . 848 860 - PsrI GAACNNNNNNTAC 872 867 840 835 866 869 + DpnI GATC 867 867 . . 866 869 + BstKTI GATC 868 866 . . 866 869 + MboI GATC 865 869 . . 868 873 + Hpy178III* TCNNGA 869 871 . . 876 879 + TaqI TCGA 876 878 . . 878 882 + TfiI GAWTC 878 881 . . 878 882 + HinfI GANTC 878 881 . . 911 914 + SetI ASST 914 910 . . 918 923 + MfeI CAATTG 918 922 . . 919 922 + TspEI AATT 918 922 . . 922 927 + TatI WGTACW 922 926 . . 922 927 + Bsp1407I TGTACA 922 926 . . 923 928 + Hpy166II GTNNAC 925 925 . . 923 926 + Csp6I GTAC 923 925 . . 923 926 + RsaI GTAC 924 924 . . 936 950 + XcmI CCANNNNNNNNNTGG 943 942 . . 936 940 + BccI CCATC 944 945 . . 940 950 + BsiYI* CCNNNNNNNGG 946 943 . . 937 941 - FokI GGATG 923 927 . . 937 941 - BseGI GGATG 936 934 . . 941 946 + BmeT110I CYCGRG 942 944 . . 941 946 + AvaI CYCGRG 941 945 . . 946 950 - BccI CCATC 940 941 . . 957 960 + AciI CCGC 957 959 . . 1007 1017 + Hin4I GAYNNNNNVTC 998 993 1030 1025 1008 1012 + Tsp4CI* ACNGT 1010 1009 . . 1009 1015 + TspRI CASTGNN 1015 1006 . . 1013 1017 + BinI* GGATC 1021 1022 . . 1014 1017 + DpnI GATC 1015 1015 . . 1014 1017 + BstKTI GATC 1016 1014 . . 1014 1017 + MboI GATC 1013 1017 . . 1017 1022 + TaqII CACCCA 1033 1031 . . 1007 1017 - Hin4I GAYNNNNNVTC 1030 1025 998 993 1016 1020 - HphI GGTGA 1008 1007 . . 1021 1026 + BtrI CACGTC 1023 1023 . . 1022 1025 + TaiI ACGT 1025 1021 . . 1022 1025 + SetI ASST 1025 1021 . . 1022 1025 + MaeII ACGT 1022 1024 . . 1032 1036 + Hpy188I TCNGA 1034 1033 . . 1035 1039 + MboII GAAGA 1047 1046 . . 1035 1040 - Ksp632I* CTCTTC 1030 1033 . . 1073 1077 - BsmAI GTCTC 1067 1071 . . 1073 1078 - Eco31I GGTCTC 1067 1071 . . 1079 1082 + MnlI CCTC 1089 1088 . . 1086 1090 - TspGWI ACGGA 1076 1074 . . 1098 1102 + TfiI GAWTC 1098 1101 . . 1095 1098 - MnlI CCTC 1088 1087 . . 1098 1102 + HinfI GANTC 1098 1101 . . 1104 1109 + TsoI TARCCA 1120 1118 . . 1105 1108 + CviJI RGCY 1106 1106 . . 1116 1119 + AciI CCGC 1116 1118 . . 1115 1120 - EciI GGCGGA 1105 1103 . . 1121 1125 + MaeIII GTNAC 1120 1125 . . 1118 1122 - BceAI ACGGC 1103 1105 . . 1127 1131 + Hpy188I TCNGA 1129 1128 . . 1125 1129 - FokI GGATG 1111 1115 . . 1125 1129 - BseGI GGATG 1124 1122 . . 1136 1140 + Tsp45I GTSAC 1135 1140 . . 1136 1140 + MaeIII GTNAC 1135 1140 . . 1137 1141 - HphI GGTGA 1129 1128 . . 1146 1149 + SetI ASST 1149 1145 . . 1147 1150 + MnlI CCTC 1157 1156 . . 1161 1164 + SetI ASST 1164 1160 . . 1161 1166 + BspMI ACCTGC 1170 1174 . . 1164 1167 + CviRI* TGCA 1165 1165 . . 1177 1180 + CviJI RGCY 1178 1178 . . 1178 1182 + TauI GCSGC 1181 1178 . . 1178 1182 + BlsI GCNGC 1180 1179 . . 1178 1182 + BisI GCNGC 1179 1180 . . 1179 1182 + AciI CCGC 1179 1181 . . 1180 1183 + FnuDII* CGCG 1181 1181 . . 1183 1186 + Csp6I GTAC 1183 1185 . . 1183 1186 + RsaI GTAC 1184 1184 . . 1189 1193 + BsrI ACTGG 1194 1192 . . 1198 1201 + FatI CATG 1197 1201 . . 1198 1201 + NlaIII CATG 1201 1197 . . 1198 1201 + CviAII CATG 1198 1200 . . 1207 1221 + XcmI CCANNNNNNNNNTGG 1214 1213 . . 1208 1213 + MfeI CAATTG 1208 1212 . . 1209 1212 + TspEI AATT 1208 1212 . . 1214 1217 + MseI TTAA 1214 1216 . . 1216 1219 + TspEI AATT 1215 1219 . . 1236 1240 + TauI GCSGC 1239 1236 . . 1236 1240 + BlsI GCNGC 1238 1237 . . 1236 1240 + BisI GCNGC 1237 1238 . . 1237 1240 + AciI CCGC 1237 1239 . . 1245 1256 + BcgI CGANNNNNNTGC 1234 1232 1268 1266 1254 1257 + CviRI* TGCA 1255 1255 . . 1253 1258 - BsgI GTGCAG 1238 1236 . . 1259 1263 + Tsp4CI* ACNGT 1261 1260 . . 1261 1266 + TatI WGTACW 1261 1265 . . 1261 1266 + ScaI AGTACT 1263 1263 . . 1262 1265 + Csp6I GTAC 1262 1264 . . 1262 1265 + RsaI GTAC 1263 1263 . . 1279 1284 + SduI GDGCHC 1283 1279 . . 1279 1284 + HgiJII* GRGCYC 1283 1279 . . 1280 1283 + CviJI RGCY 1281 1281 . . 1286 1290 + HinfI GANTC 1286 1289 . . 1286 1290 - PleI GAGTC 1280 1281 . . 1286 1290 - MlyI GAGTC 1280 1280 . . 1292 1295 + MseI TTAA 1292 1294 . . 1296 1299 + CviJI RGCY 1297 1297 . . 1313 1317 + Hpy188I TCNGA 1315 1314 . . 1319 1323 + Tsp4CI* ACNGT 1321 1320 . . 1323 1326 + MseI TTAA 1323 1325 . . 1328 1333 + AclI AACGTT 1329 1331 . . 1329 1332 + TaiI ACGT 1332 1328 . . 1329 1332 + SetI ASST 1332 1328 . . 1329 1332 + MaeII ACGT 1329 1331 . . 1337 1340 + SetI ASST 1340 1336 . . 1344 1349 + NspBII* CMGCKG 1346 1346 . . 1344 1347 + AciI CCGC 1344 1346 . . 1358 1364 + BstEII GGTNACC 1358 1363 . . 1359 1363 + MaeIII GTNAC 1358 1363 . . 1378 1382 + Tsp4CI* ACNGT 1380 1379 . . 1399 1404 + Hpy178III* TCNNGA 1400 1402 . . 1401 1404 + TaqI TCGA 1401 1403 . . 1412 1422 + Hin4I GAYNNNNNVTC 1403 1398 1435 1430 1422 1427 + NspBII* CMGCKG 1424 1424 . . 1412 1422 - Hin4I GAYNNNNNVTC 1435 1430 1403 1398 1422 1425 + AciI CCGC 1422 1424 . . 1436 1440 + HphI GGTGA 1448 1447 . . 1437 1441 + Tsp45I GTSAC 1436 1441 . . 1437 1441 + MaeIII GTNAC 1436 1441 . . 1439 1443 + HinfI GANTC 1439 1442 . . 1442 1447 + Hpy178III* TCNNGA 1443 1445 . . 1439 1443 - PleI GAGTC 1433 1434 . . 1439 1443 - MlyI GAGTC 1433 1433 . . 1441 1446 - Eco57MI CTGRAG 1426 1424 . . 1441 1446 - GsuI CTGGAG 1426 1424 . . 1459 1463 + BsmAI GTCTC 1464 1468 . . 1465 1468 + CviRI* TGCA 1466 1466 . . 1469 1479 + BsaXI ACNNNNNCTCC 1459 1456 1489 1486 1479 1484 + PstI CTGCAG 1483 1479 . . 1479 1484 + SfeI* CTRYAG 1479 1483 . . 1480 1483 + CviRI* TGCA 1481 1481 . . 1481 1485 + BlsI GCNGC 1483 1482 . . 1481 1485 + BisI GCNGC 1482 1483 . . 1481 1485 + BbvI GCAGC 1493 1497 . . 1481 1485 + TseI GCWGC 1481 1484 . . 1485 1490 + MfeI CAATTG 1485 1489 . . 1486 1489 + TspEI AATT 1485 1489 . . 1492 1496 + FauI CCCGC 1500 1502 . . 1493 1496 + AciI CCGC 1493 1495 . . 1513 1518 + SecI* CCNNGG 1513 1517 . . 1514 1518 + BssKI CCNGG 1513 1518 . . 1514 1518 + BseBI CCWGG 1515 1516 . . 1514 1518 + ScrFI CCNGG 1515 1516 . . 1514 1518 + EcoRII CCWGG 1513 1518 . . 1516 1519 + SetI ASST 1519 1515 . . 1520 1525 + TatI WGTACW 1520 1524 . . 1520 1525 + ScaI AGTACT 1522 1522 . . 1521 1524 + Csp6I GTAC 1521 1523 . . 1521 1524 + RsaI GTAC 1522 1522 . . 1529 1534 + TatI WGTACW 1529 1533 . . 1530 1533 + Csp6I GTAC 1530 1532 . . 1530 1533 + RsaI GTAC 1531 1531 . . 1537 1542 + MstI* TGCGCA 1539 1539 . . 1538 1541 + Hin6I GCGC 1538 1540 . . 1538 1541 + GlaI GCGC 1539 1539 . . 1538 1541 + HhaI GCGC 1540 1538 . . 1542 1545 + TspEI AATT 1541 1545 . . 1545 1550 + Hpy178III* TCNNGA 1546 1548 . . 1552 1555 + SetI ASST 1555 1551 . . 1554 1559 + Eco57MI CTGRAG 1575 1573 . . 1554 1559 + Eco57I CTGAAG 1575 1573 . . 1556 1560 + MboII GAAGA 1568 1567 . . 1562 1567 + Cac8I GCNNGC 1564 1564 . . 1566 1570 + SfaNI GCATC 1575 1579 . . 1570 1580 + EcoNI CCTNNNNNAGG 1574 1575 . . 1570 1580 + BsiYI* CCNNNNNNNGG 1576 1573 . . 1567 1571 - FokI GGATG 1553 1557 . . 1567 1571 - BseGI GGATG 1566 1564 . . 1571 1574 + MaeI CTAG 1571 1573 . . 1574 1578 + MboII GAAGA 1586 1585 . . 1579 1583 + FokI GGATG 1592 1596 . . 1574 1579 - Ksp632I* CTCTTC 1569 1572 . . 1579 1583 + BseGI GGATG 1585 1583 . . 1577 1580 - MnlI CCTC 1570 1569 . . 1580 1584 - BccI CCATC 1574 1575 . . 1592 1597 + MmeI TCCRAC 1617 1615 . . 1595 1600 + AclI AACGTT 1596 1598 . . 1596 1599 + TaiI ACGT 1599 1595 . . 1596 1599 + SetI ASST 1599 1595 . . 1596 1599 + MaeII ACGT 1596 1598 . . 1601 1605 + BsmAI GTCTC 1606 1610 . . 1612 1616 + Hpy188I TCNGA 1614 1613 . . 1624 1627 + SetI ASST 1627 1623 . . 1636 1641 + BclI TGATCA 1636 1640 . . 1637 1640 + DpnI GATC 1638 1638 . . 1637 1640 + BstKTI GATC 1639 1637 . . 1637 1640 + MboI GATC 1636 1640 . . 1649 1659 + FalI AAGNNNNNCTT 1640 1635 1672 1667 1655 1658 + SetI ASST 1658 1654 . . 1649 1659 - FalI AAGNNNNNCTT 1672 1667 1640 1635 1661 1664 + CviRI* TGCA 1662 1662 . . 1670 1674 - BsrI ACTGG 1670 1668 . . 1678 1682 + MaeIII GTNAC 1677 1682 . . 1676 1681 - TsoI TARCCA 1666 1664 . . 1699 1702 + DpnI GATC 1700 1700 . . 1699 1702 + BstKTI GATC 1701 1699 . . 1699 1702 + MboI GATC 1698 1702 . . 1704 1709 + SnaBI TACGTA 1706 1706 . . 1704 1709 + BsaAI YACGTR 1706 1706 . . 1705 1708 + TaiI ACGT 1708 1704 . . 1705 1708 + SetI ASST 1708 1704 . . 1705 1708 + MaeII ACGT 1705 1707 . . 1712 1715 + DpnI GATC 1713 1713 . . 1712 1715 + BstKTI GATC 1714 1712 . . 1712 1715 + MboI GATC 1711 1715 . . 1726 1729 + SetI ASST 1729 1725 . . 1726 1731 + BspMI ACCTGC 1735 1739 . . 1736 1741 + VspI ATTAAT 1737 1739 . . 1737 1740 + MseI TTAA 1737 1739 . . 1744 1748 - FokI GGATG 1730 1734 . . 1744 1748 - BseGI GGATG 1743 1741 . . 1757 1760 + FatI CATG 1756 1760 . . 1757 1760 + NlaIII CATG 1760 1756 . . 1757 1760 + CviAII CATG 1757 1759 . . 1768 1771 + TspEI AATT 1767 1771 . . 1770 1773 + MseI TTAA 1770 1772 . . 1774 1777 + SetI ASST 1777 1773 . . 1775 1779 + BssKI CCNGG 1774 1779 . . 1775 1779 + BseBI CCWGG 1776 1777 . . 1775 1779 + ScrFI CCNGG 1776 1777 . . 1775 1779 + EcoRII CCWGG 1774 1779 . . 1779 1782 + Hin6I GCGC 1779 1781 . . 1779 1782 + GlaI GCGC 1780 1780 . . 1779 1782 + HhaI GCGC 1781 1779 . . 1789 1792 + SetI ASST 1792 1788 . . 1790 1793 + MnlI CCTC 1800 1799 . . 1792 1795 + TaqI TCGA 1792 1794 . . 1796 1801 + SfeI* CTRYAG 1796 1800 . . 1800 1803 + CviJI RGCY 1801 1801 . . 1808 1811 + FatI CATG 1807 1811 . . 1808 1811 + NlaIII CATG 1811 1807 . . 1808 1811 + CviAII CATG 1808 1810 . . 1811 1815 + MaeIII GTNAC 1810 1815 . . 1860 1863 + TaqI TCGA 1860 1862 . . 1871 1875 - TspDTI ATGAA 1861 1859 . . 1881 1884 + MnlI CCTC 1891 1890 . . 1895 1898 + CviRI* TGCA 1896 1896 . . 1896 1900 + BlsI GCNGC 1898 1897 . . 1896 1900 + BisI GCNGC 1897 1898 . . 1896 1900 + BbvI GCAGC 1908 1912 . . 1896 1900 + TseI GCWGC 1896 1899 . . 1898 1901 + SetI ASST 1901 1897 . . 1898 1901 + CviJI RGCY 1899 1899 . . 1898 1901 + AluI AGCT 1899 1899 . . 1905 1910 + SfeI* CTRYAG 1905 1909 . . 1912 1915 + MnlI CCTC 1922 1921 . . 1917 1922 + Hpy178III* TCNNGA 1918 1920 . . 1916 1920 - BslFI GGGAC 1901 1905 . . 1921 1925 + MboII GAAGA 1933 1932 . . 1929 1933 + Tsp4CI* ACNGT 1931 1930 . . 1931 1936 + KpnI GGTACC 1935 1931 . . 1931 1936 + NlaIV GGNNCC 1933 1933 . . 1931 1936 + HgiCI* GGYRCC 1931 1935 . . 1931 1936 + Acc65I GGTACC 1931 1935 . . 1932 1935 + Csp6I GTAC 1932 1934 . . 1932 1935 + RsaI GTAC 1933 1933 . . 1934 1937 + SetI ASST 1937 1933 . . 1935 1939 + Hin4II* CCTTC 1945 1944 . . 1938 1943 + MmeI TCCRAC 1963 1961 . . 1938 1942 + Hpy188I TCNGA 1940 1939 . . 1941 1945 + HinfI GANTC 1941 1944 . . 1941 1951 + Hin4I GAYNNNNNVTC 1932 1927 1964 1959 1941 1951 + Eam1105I GACNNNNNGTC 1946 1945 . . 1941 1945 - PleI GAGTC 1935 1936 . . 1941 1945 - MlyI GAGTC 1935 1935 . . 1948 1952 + Hpy99I CGWCG 1952 1947 . . 1950 1953 + TaqI TCGA 1950 1952 . . 1941 1951 - Hin4I GAYNNNNNVTC 1964 1959 1932 1927 1947 1951 - HgaI GACGC 1936 1941 . . 1957 1960 + AciI CCGC 1957 1959 . . 1972 1975 + SetI ASST 1975 1971 . . 1972 1975 + CviJI RGCY 1973 1973 . . 1972 1975 + AluI AGCT 1973 1973 . . 1977 1981 + BccI CCATC 1985 1986 . . 1982 1987 + MmeI TCCRAC 2007 2005 . . 1982 1986 + Hpy188I TCNGA 1984 1983 . . 2004 2008 + TfiI GAWTC 2004 2007 . . 2004 2008 + HinfI GANTC 2004 2007 . . 2007 2010 + TaqI TCGA 2007 2009 . . 2013 2016 + SetI ASST 2016 2012 . . 2021 2024 + CviJI RGCY 2022 2022 . . 2022 2026 - BceAI ACGGC 2007 2009 . . 2053 2063 + MwoI GCNNNNNNNGC 2059 2056 . . 2054 2057 + MaeI CTAG 2054 2056 . . 2061 2064 + CviRI* TGCA 2062 2062 . . 2069 2075 + BstEII GGTNACC 2069 2074 . . 2070 2074 + MaeIII GTNAC 2069 2074 . . 2068 2073 - TsoI TARCCA 2058 2056 . . 2079 2084 + XhoII RGATCY 2079 2083 . . 2080 2083 + DpnI GATC 2081 2081 . . 2080 2083 + BstKTI GATC 2082 2080 . . 2080 2083 + MboI GATC 2079 2083 . . 2082 2087 + Hpy178III* TCNNGA 2083 2085 . . 2080 2084 - BinI* GGATC 2074 2075 . . 2089 2094 + NlaIV GGNNCC 2091 2091 . . 2086 2090 - BccI CCATC 2080 2081 . . 2101 2104 + AciI CCGC 2101 2103 . . 2112 2115 + AciI CCGC 2112 2114 . . 2120 2124 + Tsp45I GTSAC 2119 2124 . . 2120 2124 + MaeIII GTNAC 2119 2124 . . 2126 2130 + BccI CCATC 2134 2135 . . 2129 2134 + BetI* WCCGGW 2129 2133 . . 2130 2133 + HpaII CCGG 2130 2132 . . 2127 2131 - FokI GGATG 2113 2117 . . 2127 2131 - BseGI GGATG 2126 2124 . . 2139 2143 + TspDTI ATGAA 2154 2152 . . 2147 2151 + Tsp4CI* ACNGT 2149 2148 . . 2152 2155 + FatI CATG 2151 2155 . . 2152 2155 + NlaIII CATG 2155 2151 . . 2152 2155 + CviAII CATG 2152 2154 . . 2162 2165 + CviJI RGCY 2163 2163 . . 2164 2168 + DdeI CTNAG 2164 2167 . . 2173 2176 + CviRI* TGCA 2174 2174 . . 2176 2179 + SetI ASST 2179 2175 . . 2178 2181 + MaeI CTAG 2178 2180 . . 2180 2185 + TatI WGTACW 2180 2184 . . 2181 2184 + Csp6I GTAC 2181 2183 . . 2181 2184 + RsaI GTAC 2182 2182 . . 2197 2200 + FatI CATG 2196 2200 . . 2197 2200 + NlaIII CATG 2200 2196 . . 2197 2200 + CviAII CATG 2197 2199 . . 2200 2203 + CviJI RGCY 2201 2201 . . 2217 2220 + SetI ASST 2220 2216 . . 2214 2218 - BsmAI GTCTC 2208 2212 . . 2219 2223 + DdeI CTNAG 2219 2222 . . 2214 2219 - Eco31I GGTCTC 2208 2212 . . 2220 2231 + BdaI TGANNNNNNTCA 2209 2207 2243 2241 2223 2227 + TfiI GAWTC 2223 2226 . . 2223 2227 + HinfI GANTC 2223 2226 . . 2219 2223 - BspCNI CTCAG 2211 2209 . . 2219 2223 - BseMII CTCAG 2210 2208 . . 2220 2231 - BdaI TGANNNNNNTCA 2243 2241 2209 2207 2234 2239 + Hpy166II GTNNAC 2236 2236 . . 2238 2241 + SetI ASST 2241 2237 . . 2251 2256 + VspI ATTAAT 2252 2254 . . 2252 2255 + MseI TTAA 2252 2254 . . 2254 2259 + SspI AATATT 2256 2256 . . 2261 2265 + Hpy188I TCNGA 2263 2262 . . 2264 2268 + TfiI GAWTC 2264 2267 . . 2264 2268 + HinfI GANTC 2264 2267 . . 2269 2274 + Hpy178III* TCNNGA 2270 2272 . . 2270 2284 + XcmI CCANNNNNNNNNTGG 2277 2276 . . 2273 2277 + MboII GAAGA 2285 2284 . . 2268 2273 - Eco57MI CTGRAG 2253 2251 . . 2268 2273 - GsuI CTGGAG 2253 2251 . . 2297 2301 + Tsp4CI* ACNGT 2299 2298 . . 2300 2303 + Csp6I GTAC 2300 2302 . . 2300 2303 + RsaI GTAC 2301 2301 . . 2303 2306 + MaeI CTAG 2303 2305 . . 2312 2315 + MseI TTAA 2312 2314 . . 2325 2328 + SetI ASST 2328 2324 . . 2326 2329 + MnlI CCTC 2336 2335 . . 2334 2337 + MseI TTAA 2334 2336 . . 2338 2342 + MboII GAAGA 2350 2349 . . 2351 2356 + ApoI RAATTY 2351 2355 . . 2352 2355 + TspEI AATT 2351 2355 . . 2356 2359 + CviRI* TGCA 2357 2357 . . 2357 2361 + SfaNI GCATC 2366 2370 . . 2360 2364 + Hpy188I TCNGA 2362 2361 . . 2361 2366 + Eco57MI CTGRAG 2382 2380 . . 2361 2366 + Eco57I CTGAAG 2382 2380 . . 2363 2367 + MboII GAAGA 2375 2374 . . 2370 2374 + DdeI CTNAG 2370 2373 . . 2370 2374 - BspCNI CTCAG 2362 2360 . . 2370 2374 - BseMII CTCAG 2361 2359 . . 2381 2384 + CviJI RGCY 2382 2382 . . 2383 2388 + SfeI* CTRYAG 2383 2387 . . 2394 2398 + TfiI GAWTC 2394 2397 . . 2394 2398 + HinfI GANTC 2394 2397 . . 2397 2402 + Hpy178III* TCNNGA 2398 2400 . . 2407 2410 + SetI ASST 2410 2406 . . 2407 2410 + CviJI RGCY 2408 2408 . . 2407 2410 + AluI AGCT 2408 2408 . . 2414 2419 + SfeI* CTRYAG 2414 2418 . . 2419 2430 + AlfI GCANNNNNNTGC 2408 2406 2442 2440 2420 2429 + MslI CAYNNNNRTG 2424 2424 . . 2420 2429 + OliI CACNNNNGTG 2424 2424 . . 2427 2432 + BsgI GTGCAG 2448 2446 . . 2428 2431 + CviRI* TGCA 2429 2429 . . 2429 2433 + BlsI GCNGC 2431 2430 . . 2429 2433 + BisI GCNGC 2430 2431 . . 2429 2433 + BbvI GCAGC 2441 2445 . . 2429 2433 + TseI GCWGC 2429 2432 . . 2419 2430 - AlfI GCANNNNNNTGC 2442 2440 2408 2406 2436 2440 + Hpy188I TCNGA 2438 2437 . . 2439 2443 - BsmAI GTCTC 2433 2437 . . 2446 2451 + MmeI TCCRAC 2471 2469 . . 2446 2450 + Hpy188I TCNGA 2448 2447 . . 2448 2452 + Hpy99I CGWCG 2452 2447 . . 2449 2453 + HgaI GACGC 2458 2463 . . 2449 2454 + AcyI GRCGYC 2450 2452 . . 2459 2463 + Tsp4CI* ACNGT 2461 2460 . . 2492 2497 + VspI ATTAAT 2493 2495 . . 2493 2496 + MseI TTAA 2493 2495 . . 2495 2498 + TspEI AATT 2494 2498 . . 2500 2505 + SfeI* CTRYAG 2500 2504 . . 2508 2512 + TfiI GAWTC 2508 2511 . . 2508 2512 + HinfI GANTC 2508 2511 . . 2510 2514 - TspDTI ATGAA 2500 2498 . . 2519 2522 + TspEI AATT 2518 2522 . . 2533 2536 + CviRI* TGCA 2534 2534 . . 2535 2546 + TstI CACNNNNNNTCC 2526 2521 2558 2553 2538 2541 + CviJI RGCY 2539 2539 . . 2544 2549 + MmeI TCCRAC 2569 2567 . . 2548 2552 + Tsp4CI* ACNGT 2550 2549 . . 2559 2563 + Tsp45I GTSAC 2558 2563 . . 2559 2563 + MaeIII GTNAC 2558 2563 . . 2560 2571 + BdaI TGANNNNNNTCA 2549 2547 2583 2581 2561 2570 + PshAI GACNNNNGTC 2565 2565 . . 2560 2571 - BdaI TGANNNNNNTCA 2583 2581 2549 2547 2595 2600 + MfeI CAATTG 2595 2599 . . 2596 2599 + TspEI AATT 2595 2599 . . 2612 2616 + HgaI GACGC 2621 2626 . . 2612 2617 + AcyI GRCGYC 2613 2615 . . 2622 2626 + MaeIII GTNAC 2621 2626 . . 2636 2639 + MaeI CTAG 2636 2638 . . 2639 2642 + Csp6I GTAC 2639 2641 . . 2639 2642 + RsaI GTAC 2640 2640 . . 2678 2682 + HgaI GACGC 2687 2692 . . 2678 2683 + AcyI GRCGYC 2679 2681 . . 2682 2685 + MnlI CCTC 2692 2691 . . 2691 2695 + BceAI ACGGC 2707 2709 . . 2707 2712 + XbaI TCTAGA 2707 2711 . . 2707 2712 + Hpy178III* TCNNGA 2708 2710 . . 2708 2711 + MaeI CTAG 2708 2710 . . 2711 2715 + MboII GAAGA 2723 2722 . . 2717 2722 + SalI GTCGAC 2717 2721 . . 2717 2722 + Hpy166II GTNNAC 2719 2719 . . 2717 2722 + HindII GTYRAC 2719 2719 . . 2717 2722 + AccI GTMKAC 2718 2720 . . 2718 2721 + TaqI TCGA 2718 2720 . . 2746 2750 + Hpy99I CGWCG 2750 2745 . . 2751 2755 + MaeIII GTNAC 2750 2755 . . 2767 2771 + DdeI CTNAG 2767 2770 . . 2775 2780 + SduI GDGCHC 2779 2775 . . 2775 2780 + BseSI GKGCMC 2779 2775 . . 2795 2800 + Cac8I GCNNGC 2797 2797 . . 2793 2798 - EcoP15I CAGCAG 2765 2767 . . 2802 2805 + TspEI AATT 2801 2805 . . 2818 2821 + TspEI AATT 2817 2821 . . 2841 2845 + Hpy188I TCNGA 2843 2842 . . 2856 2860 + TspGWI ACGGA 2871 2869 . . 2870 2873 + TaiI ACGT 2873 2869 . . 2870 2873 + SetI ASST 2873 2869 . . 2870 2873 + MaeII ACGT 2870 2872 . . 2873 2876 + MseI TTAA 2873 2875 . . 2921 2925 + FauI CCCGC 2929 2931 . . 2922 2925 + AciI CCGC 2922 2924 . . 2927 2931 + Hpy99I CGWCG 2931 2926 . . 2928 2932 + HgaI GACGC 2937 2942 . . 2929 2934 + MluI ACGCGT 2929 2933 . . 2929 2934 + AflIII ACRYGT 2929 2933 . . 2930 2933 + FnuDII* CGCG 2931 2931 . . 2931 2941 + MwoI GCNNNNNNNGC 2937 2934 . . 2931 2936 + Cac8I GCNNGC 2933 2933 . . 2934 2937 + CviRI* TGCA 2935 2935 . . 2942 2947 - TsoI TARCCA 2932 2930 . . 2952 2955 + SetI ASST 2955 2951 . . 2954 2957 + MaeI CTAG 2954 2956 . . 2955 2960 + TsoI TARCCA 2971 2969 . . 2956 2959 + CviJI RGCY 2957 2957 . . 2961 2966 + NdeI CATATG 2962 2964 . . 2972 2975 + SetI ASST 2975 2971 . . 2974 2978 + DdeI CTNAG 2974 2977 . . 2970 2974 - HphI GGTGA 2962 2961 . . 2982 2985 + SetI ASST 2985 2981 . . 2984 2987 + MaeI CTAG 2984 2986 . . 2986 2989 + SetI ASST 2989 2985 . . 2986 2989 + CviJI RGCY 2987 2987 . . 2986 2989 + AluI AGCT 2987 2987 . . 2990 2995 + NlaIV GGNNCC 2992 2992 . . 2990 2994 + BmgT120I GGNCC 2991 2992 . . 2990 2994 + AvaII GGWCC 2990 2993 . . 2990 2994 + AsuI* GGNCC 2990 2993 . . 2999 3004 + AclI AACGTT 3000 3002 . . 3000 3003 + TaiI ACGT 3003 2999 . . 3000 3003 + SetI ASST 3003 2999 . . 3000 3003 + MaeII ACGT 3000 3002 . . 3011 3021 + Hin4I GAYNNNNNVTC 3002 2997 3034 3029 3011 3015 - SfaNI GCATC 3001 3005 . . 3011 3021 - Hin4I GAYNNNNNVTC 3034 3029 3002 2997 3026 3030 - BceAI ACGGC 3011 3013 . . 3053 3058 + Hpy178III* TCNNGA 3054 3056 . . 3051 3055 - FokI GGATG 3037 3041 . . 3051 3055 - BseGI GGATG 3050 3048 . . 3058 3061 + SetI ASST 3061 3057 . . 3063 3066 + TaqI TCGA 3063 3065 . . 3074 3077 + SetI ASST 3077 3073 . . 3074 3077 + CviJI RGCY 3075 3075 . . 3074 3077 + AluI AGCT 3075 3075 . . 3095 3099 + Hpy188I TCNGA 3097 3096 . . 3119 3122 + MseI TTAA 3119 3121 . . 3121 3124 + TspEI AATT 3120 3124 . . 3125 3130 + NdeI CATATG 3126 3128 . . 3123 3127 - TspDTI ATGAA 3113 3111 . . 3128 3132 + TspDTI ATGAA 3143 3141 . . 3135 3138 + TspEI AATT 3134 3138 . . 3150 3153 + TaiI ACGT 3153 3149 . . 3150 3153 + SetI ASST 3153 3149 . . 3150 3153 + MaeII ACGT 3150 3152 . . 3152 3156 + Tsp45I GTSAC 3151 3156 . . 3152 3156 + MaeIII GTNAC 3151 3156 . . 3157 3160 + SetI ASST 3160 3156 . . 3159 3164 + Eco57MI CTGRAG 3180 3178 . . 3159 3164 + Eco57I CTGAAG 3180 3178 . . 3161 3165 + MboII GAAGA 3173 3172 . . 3172 3175 + CviJI RGCY 3173 3173 . . 3174 3178 + Hin4II* CCTTC 3184 3183 . . 3177 3181 - TspGWI ACGGA 3167 3165 . . 3183 3187 + MaeIII GTNAC 3182 3187 . . 3190 3194 + MboII GAAGA 3202 3201 . . 3198 3201 + TaqI TCGA 3198 3200 . . 3206 3210 + Hpy188I TCNGA 3208 3207 . . 3212 3215 + SetI ASST 3215 3211 . . 3213 3217 + Hin4II* CCTTC 3223 3222 . . 3209 3213 - BsmAI GTCTC 3203 3207 . . 3209 3214 - Eco31I GGTCTC 3203 3207 . . 3216 3220 + Hpy188I TCNGA 3218 3217 . . 3224 3227 + TspEI AATT 3223 3227 . . 3233 3236 + MaeI CTAG 3233 3235 . . 3236 3240 + MboII GAAGA 3248 3247 . . 3236 3241 + BbvII* GAAGAC 3243 3247 . . 3247 3250 + DpnI GATC 3248 3248 . . 3247 3250 + BstKTI GATC 3249 3247 . . 3247 3250 + MboI GATC 3246 3250 . . 3260 3265 + TaqII GACCGA 3276 3274 . . 3265 3270 + SpeI ACTAGT 3265 3269 . . 3266 3269 + MaeI CTAG 3266 3268 . . 3272 3275 + Csp6I GTAC 3272 3274 . . 3272 3275 + RsaI GTAC 3273 3273 . . 3301 3305 - BsmAI GTCTC 3295 3299 . . 3310 3315 + Hpy178III* TCNNGA 3311 3313 . . 3310 3315 + BspHI TCATGA 3310 3314 . . 3301 3311 - Hin4I GAYNNNNNVTC 3324 3319 3292 3287 3311 3314 + FatI CATG 3310 3314 . . 3311 3314 + NlaIII CATG 3314 3310 . . 3311 3314 + CviAII CATG 3311 3313 . . 3316 3330 + XcmI CCANNNNNNNNNTGG 3323 3322 . . 3320 3323 + SetI ASST 3323 3319 . . 3330 3333 - MnlI CCTC 3323 3322 . . 3335 3344 + MslI CAYNNNNRTG 3339 3339 . . 3344 3348 + MaeIII GTNAC 3343 3348 . . 3347 3351 + Tsp4CI* ACNGT 3349 3348 . . 3348 3354 + TspRI CASTGNN 3354 3345 . . 3350 3354 + Tsp45I GTSAC 3349 3354 . . 3350 3354 + MaeIII GTNAC 3349 3354 . . 3368 3371 + CviJI RGCY 3369 3369 . . 3371 3385 + XcmI CCANNNNNNNNNTGG 3378 3377 . . 3373 3376 + TspEI AATT 3372 3376 . . 3383 3388 + BalI TGGCCA 3385 3385 . . 3383 3388 + CfrI YGGCCR 3383 3387 . . 3384 3387 + CviJI RGCY 3385 3385 . . 3384 3387 + HaeIII GGCC 3385 3385 . . 3390 3393 + TspEI AATT 3389 3393 . . 3401 3404 + TaqI TCGA 3401 3403 . . 3403 3406 + DpnI GATC 3404 3404 . . 3403 3406 + BstKTI GATC 3405 3403 . . 3403 3406 + MboI GATC 3402 3406 . . 3417 3422 + Hpy178III* TCNNGA 3418 3420 . . 3418 3426 + AlwNI CAGNNNCTG 3423 3420 . . 3419 3425 + PpuMI RGGWCCY 3420 3423 . . 3419 3425 + DraII RGGNCCY 3420 3423 . . 3420 3424 + BmgT120I GGNCC 3421 3422 . . 3420 3424 + AvaII GGWCC 3420 3423 . . 3420 3424 + AsuI* GGNCC 3420 3423 . . 3422 3425 + SetI ASST 3425 3421 . . 3422 3427 + BspMI ACCTGC 3431 3435 . . 3428 3431 + Csp6I GTAC 3428 3430 . . 3428 3431 + RsaI GTAC 3429 3429 . . 3435 3440 + Hpy166II GTNNAC 3437 3437 . . 3435 3440 + HindII GTYRAC 3437 3437 . . 3467 3470 + FatI CATG 3466 3470 . . 3467 3470 + NlaIII CATG 3470 3466 . . 3467 3470 + CviAII CATG 3467 3469 . . 3478 3488 + BsaXI ACNNNNNCTCC 3468 3465 3498 3495 3489 3494 + SfeI* CTRYAG 3489 3493 . . 3501 3505 + MboII GAAGA 3513 3512 . . 3497 3501 - Hin4II* CCTTC 3491 3490 . . 3507 3510 + CviJI RGCY 3508 3508 . . 3504 3508 - BccI CCATC 3498 3499 . . 3530 3534 + Tsp45I GTSAC 3529 3534 . . 3530 3534 + MaeIII GTNAC 3529 3534 . . 3568 3571 + DpnI GATC 3569 3569 . . 3568 3571 + BstKTI GATC 3570 3568 . . 3568 3571 + MboI GATC 3567 3571 . . 3571 3574 + MnlI CCTC 3581 3580 . . 3568 3572 - BinI* GGATC 3562 3563 . . 3574 3578 + Hpy99I CGWCG 3578 3573 . . 3584 3587 + DpnI GATC 3585 3585 . . 3584 3587 + BstKTI GATC 3586 3584 . . 3584 3587 + MboI GATC 3583 3587 . . 3592 3597 + AsuII TTCGAA 3593 3595 . . 3593 3596 + TaqI TCGA 3593 3595 . . 3599 3602 + FnuDII* CGCG 3600 3600 . . 3600 3603 + Hin6I GCGC 3600 3602 . . 3600 3603 + GlaI GCGC 3601 3601 . . 3600 3603 + HhaI GCGC 3602 3600 . . 3608 3612 - TspDTI ATGAA 3598 3596 . . 3613 3616 + SetI ASST 3616 3612 . . 3613 3616 + CviJI RGCY 3614 3614 . . 3613 3616 + AluI AGCT 3614 3614 . . 3626 3629 + SetI ASST 3629 3625 . . 3628 3632 + DdeI CTNAG 3628 3631 . . 3632 3636 + HgaI GACGC 3641 3646 . . 3649 3654 + XhoII RGATCY 3649 3653 . . 3649 3654 + BglII AGATCT 3649 3653 . . 3650 3653 + DpnI GATC 3651 3651 . . 3650 3653 + BstKTI GATC 3652 3650 . . 3650 3653 + MboI GATC 3649 3653 . . 3661 3664 + TaqI TCGA 3661 3663 . . 3673 3677 - TspDTI ATGAA 3663 3661 . . 3680 3683 + FatI CATG 3679 3683 . . 3680 3683 + NlaIII CATG 3683 3679 . . 3680 3683 + CviAII CATG 3680 3682 . . 3689 3694 + SecI* CCNNGG 3689 3693 . . 3690 3694 + BssKI CCNGG 3689 3694 . . 3690 3694 + BseBI CCWGG 3691 3692 . . 3690 3694 + ScrFI CCNGG 3691 3692 . . 3690 3694 + EcoRII CCWGG 3689 3694 . . 3699 3704 + TsoI TARCCA 3715 3713 . . 3703 3709 + TspRI CASTGNN 3709 3700 . . 3702 3706 - BsrI ACTGG 3702 3700 . . 3717 3721 - TspGWI ACGGA 3707 3705 . . 3726 3729 + AciI CCGC 3726 3728 . . 3739 3744 + Hpy178III* TCNNGA 3740 3742 . . 3747 3752 + Hpy178III* TCNNGA 3748 3750 . . 3758 3761 + MaeI CTAG 3758 3760 . . 3770 3773 + TaqI TCGA 3770 3772 . . 3772 3780 + Tth111I GACNNNGTC 3775 3776 . . 3779 3783 - MboII GAAGA 3771 3770 . . 3778 3783 - BbvII* GAAGAC 3771 3775 . . 3783 3786 + AciI CCGC 3783 3785 . . 3784 3787 + FnuDII* CGCG 3785 3785 . . 3796 3807 + BstXI CCANNNNNNTGG 3803 3799 . . 3803 3813 + Hin4I GAYNNNNNVTC 3794 3789 3826 3821 3803 3807 - BccI CCATC 3797 3798 . . 3812 3816 + Hpy188I TCNGA 3814 3813 . . 3803 3813 - Hin4I GAYNNNNNVTC 3826 3821 3794 3789 3815 3827 + PsrI GAACNNNNNNTAC 3807 3802 3839 3834 3833 3836 + MseI TTAA 3833 3835 . . 3839 3842 + SetI ASST 3842 3838 . . 3841 3845 + MaeIII GTNAC 3840 3845 . . 3845 3849 + DdeI CTNAG 3845 3848 . . 3850 3853 + CviJI RGCY 3851 3851 . . 3866 3871 + SspI AATATT 3868 3868 . . 3871 3875 + Hpy188I TCNGA 3873 3872 . . 3875 3878 + TspEI AATT 3874 3878 . . 3880 3885 + NspI RCATGY 3884 3880 . . 3881 3884 + FatI CATG 3880 3884 . . 3881 3884 + NlaIII CATG 3884 3880 . . 3881 3884 + CviAII CATG 3881 3883 . . 3900 3903 + TaqI TCGA 3900 3902 . . 3902 3907 + ApoI RAATTY 3902 3906 . . 3903 3906 + TspEI AATT 3902 3906 . . 3908 3913 + Hpy166II GTNNAC 3910 3910 . . 3914 3917 + TspEI AATT 3913 3917 . . 3922 3925 + SetI ASST 3925 3921 . . 3927 3930 + MaeI CTAG 3927 3929 . . 3944 3948 - HphI GGTGA 3936 3935 . . 3955 3958 + TspEI AATT 3954 3958 . . 3990 3993 + MseI TTAA 3990 3992 . . 3995 3999 + Hpy188I TCNGA 3997 3996 . . 4001 4009 + Tth111I GACNNNGTC 4004 4005 . . 4007 4012 + Hpy166II GTNNAC 4009 4009 . . 4007 4012 + HindII GTYRAC 4009 4009 . . 4028 4032 - BceAI ACGGC 4013 4015 . . 4047 4050 + SetI ASST 4050 4046 . . 4048 4051 + MnlI CCTC 4058 4057 . . 4054 4057 + CviJI RGCY 4055 4055 . . 4062 4066 + BccI CCATC 4070 4071 . . 4066 4076 + BsiYI* CCNNNNNNNGG 4072 4069 . . 4063 4067 - FokI GGATG 4049 4053 . . 4063 4067 - BseGI GGATG 4062 4060 . . 4072 4076 - Hin4II* CCTTC 4066 4065 . . 4080 4083 + SetI ASST 4083 4079 . . 4080 4083 + CviJI RGCY 4081 4081 . . 4080 4083 + AluI AGCT 4081 4081 . . 4082 4085 + MaeI CTAG 4082 4084 . . 4098 4101 + TaqI TCGA 4098 4100 . . 4126 4129 + TaiI ACGT 4129 4125 . . 4126 4129 + SetI ASST 4129 4125 . . 4126 4129 + MaeII ACGT 4126 4128 . . 4128 4133 + Hpy166II GTNNAC 4130 4130 . . 4142 4145 + MseI TTAA 4142 4144 . . 4154 4157 + DpnI GATC 4155 4155 . . 4154 4157 + BstKTI GATC 4156 4154 . . 4154 4157 + MboI GATC 4153 4157 . . 4157 4162 + BsaAI YACGTR 4159 4159 . . 4158 4161 + TaiI ACGT 4161 4157 . . 4158 4161 + SetI ASST 4161 4157 . . 4158 4161 + MaeII ACGT 4158 4160 . . 4151 4161 - BsaXI ACNNNNNCTCC 4173 4170 4143 4140 4167 4170 + CviRI* TGCA 4168 4168 . . 4168 4179 + AlfI GCANNNNNNTGC 4157 4155 4191 4189 4169 4178 + MslI CAYNNNNRTG 4173 4173 . . 4176 4181 + EcoT22I ATGCAT 4180 4176 . . 4177 4180 + CviRI* TGCA 4178 4178 . . 4175 4179 - SfaNI GCATC 4165 4169 . . 4168 4179 - AlfI GCANNNNNNTGC 4191 4189 4157 4155 4185 4190 + Hpy178III* TCNNGA 4186 4188 . . 4192 4195 + SetI ASST 4195 4191 . . 4202 4206 + TspDTI ATGAA 4217 4215 . . 4207 4212 + TatI WGTACW 4207 4211 . . 4208 4211 + Csp6I GTAC 4208 4210 . . 4208 4211 + RsaI GTAC 4209 4209 . . 4221 4224 + TaiI ACGT 4224 4220 . . 4221 4224 + SetI ASST 4224 4220 . . 4221 4224 + MaeII ACGT 4221 4223 . . 4222 4226 + Hpy99I CGWCG 4226 4221 . . 4224 4228 + Hpy188I TCNGA 4226 4225 . . 4226 4230 + BmgT120I GGNCC 4227 4228 . . 4226 4230 + AvaII GGWCC 4226 4229 . . 4226 4230 + AsuI* GGNCC 4226 4229 . . 4223 4228 - MmeI TCCRAC 4204 4202 . . 4234 4237 + TaqI TCGA 4234 4236 . . 4242 4247 + Hpy178III* TCNNGA 4243 4245 . . 4271 4274 + MaeI CTAG 4271 4273 . . 4274 4284 + Hin4I GAYNNNNNVTC 4265 4260 4297 4292 4276 4281 + AhaIII* TTTAAA 4278 4278 . . 4277 4280 + MseI TTAA 4277 4279 . . 4307 4312 + VspI ATTAAT 4308 4310 . . 4308 4311 + MseI TTAA 4308 4310 . . 4312 4317 + TatI WGTACW 4312 4316 . . 4312 4317 + Bsp1407I TGTACA 4312 4316 . . 4313 4316 + Csp6I GTAC 4313 4315 . . 4313 4316 + RsaI GTAC 4314 4314 . . 4317 4320 + TspEI AATT 4316 4320 . . 4320 4325 + Hpy178III* TCNNGA 4321 4323 . . 4334 4339 + Hpy166II GTNNAC 4336 4336 . . 4334 4339 + BssNAI GTATAC 4336 4336 . . 4334 4339 + AccI GTMKAC 4335 4337 . . 4338 4342 + Tsp4CI* ACNGT 4340 4339 . . 4342 4347 + Hpy178III* TCNNGA 4343 4345 . . 4346 4350 + HgaI GACGC 4355 4360 . . 4347 4352 + MluI ACGCGT 4347 4351 . . 4347 4352 + AflIII ACRYGT 4347 4351 . . 4348 4351 + FnuDII* CGCG 4349 4349 . . 4351 4354 + Csp6I GTAC 4351 4353 . . 4351 4354 + RsaI GTAC 4352 4352 . . 4373 4376 + SetI ASST 4376 4372 . . 4373 4376 + CviJI RGCY 4374 4374 . . 4373 4376 + AluI AGCT 4374 4374 . . 4378 4384 + TspRI CASTGNN 4384 4375 . . 4387 4390 + TspEI AATT 4386 4390 . . 4389 4392 + MseI TTAA 4389 4391 . . 4395 4400 + SduI GDGCHC 4399 4395 . . 4395 4400 + HgiAI* GWGCWC 4399 4395 . . 4402 4413 + BcgI CGANNNNNNTGC 4391 4389 4425 4423 4406 4410 + MaeIII GTNAC 4405 4410 . . 4411 4414 + CviRI* TGCA 4412 4412 . . 4410 4415 - BsgI GTGCAG 4395 4393 . . 4452 4456 + Tsp45I GTSAC 4451 4456 . . 4452 4456 + MaeIII GTNAC 4451 4456 . . 4455 4460 + NspI RCATGY 4459 4455 . . 4456 4459 + FatI CATG 4455 4459 . . 4456 4459 + NlaIII CATG 4459 4455 . . 4456 4459 + CviAII CATG 4456 4458 . . 4458 4461 + CviRI* TGCA 4459 4459 . . 4466 4471 + Hpy166II GTNNAC 4468 4468 . . 4466 4471 + AccI GTMKAC 4467 4469 . . 4492 4496 + DdeI CTNAG 4492 4495 . . 4492 4496 + BspCNI CTCAG 4505 4503 . . 4492 4496 + BseMII CTCAG 4506 4504 . . 4493 4497 + Hpy188I TCNGA 4495 4494 . . 4494 4502 + AlwNI CAGNNNCTG 4499 4496 . . 4498 4501 + SetI ASST 4501 4497 . . 4498 4501 + CviJI RGCY 4499 4499 . . 4498 4501 + AluI AGCT 4499 4499 . . 4502 4507 + ApoI RAATTY 4502 4506 . . 4503 4506 + TspEI AATT 4502 4506 . . 4512 4517 + ApoI RAATTY 4512 4516 . . 4513 4516 + TspEI AATT 4512 4516 . . 4516 4521 + Hpy178III* TCNNGA 4517 4519 . . 4530 4534 + MboII GAAGA 4542 4541 . . 4533 4536 + DpnI GATC 4534 4534 . . 4533 4536 + BstKTI GATC 4535 4533 . . 4533 4536 + MboI GATC 4532 4536 . . 4537 4542 + SspI AATATT 4539 4539 . . 4542 4547 + TsoI TARCCA 4558 4556 . . 4543 4546 + CviJI RGCY 4544 4544 . . 4581 4585 + DdeI CTNAG 4581 4584 . . 4581 4585 - BspCNI CTCAG 4573 4571 . . 4581 4585 - BseMII CTCAG 4572 4570 . . 4589 4592 - MnlI CCTC 4582 4581 . . 4592 4596 + MboII GAAGA 4604 4603 . . 4595 4605 + Hin4I GAYNNNNNVTC 4586 4581 4618 4613 4595 4605 - Hin4I GAYNNNNNVTC 4618 4613 4586 4581 4612 4623 + BdaI TGANNNNNNTCA 4601 4599 4635 4633 4612 4623 - BdaI TGANNNNNNTCA 4635 4633 4601 4599 4628 4638 + Hin4I GAYNNNNNVTC 4619 4614 . . 4628 4638 + Eam1105I GACNNNNNGTC 4633 4632 . . 4634 4637 + TaiI ACGT 4637 4633 . . 4634 4637 + SetI ASST 4637 4633 . . 4634 4637 + MaeII ACGT 4634 4636 . . 4636 4640 + Tsp45I GTSAC 4635 4640 . . 4636 4640 + MaeIII GTNAC 4635 4640 . . 4637 4641 - HphI GGTGA 4629 4628 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 1931 2714 # # AccI: # [GTMKAC] # 132 178 261 1617 2457 # # AciI: # [CCGC] # 11 57 58 63 71 78 # 107 144 159 221 464 736 # 804 810 862 # # AclI: # [AACGTT] # 267 1329 1404 1645 # # AcyI: # [GRCGYC] # 66 163 530 1920 1966 # # AflIII: # [ACRYGT] # 152 298 1418 2777 # # AhaIII*: # [TTTAAA] # 367 4278 # # AjuI: # [GAANNNNNNNTTGG] # 32 356 4257 # # AlfI: # [GCANNNNNNTGC] # 0 0 0 0 34 34 # 454 1715 2408 # # AluI: # [AGCT] # 5 74 81 88 125 146 # 293 362 435 467 539 579 # 1451 # # AlwNI: # [CAGNNNCTG] # 146 1076 3423 # # ApoI: # [RAATTY] # 10 133 600 1551 2351 # # AsuI*: # [GGNCC] # 419 430 806 2990 # # AsuII: # [TTCGAA] # 587 1052 3006 # # AvaI: # [CYCGRG] # 941 3704 # # AvaII: # [GGWCC] # 419 430 806 2990 # # BalI: # [TGGCCA] # 1260 3385 # # BarI: # [GAAGNNNNNNTAC] # 32 44 4569 # # BbvI: # [GCAGC] # 210 225 415 533 1058 2204 # # BbvII*: # [GAAGAC] # 210 528 874 3033 # # BccI: # [CCATC] # 4 54 90 95 166 179 # 273 299 411 505 575 630 # 1364 # # BceAI: # [ACGGC] # 304 632 700 904 1002 1103 # # BcgI: # [CGANNNNNNTGC] # 34 34 34 34 113 220 # 430 623 3123 # # BciVI: # [GTATCC] # 682 3963 # # BclI: # [TGATCA] # 1636 3009 # # BdaI: # [TGANNNNNNTCA] # 0 0 0 0 0 0 # 10 34 34 34 306 2018 # 2209 # # BetI*: # [WCCGGW] # 2129 2516 # # BfiI: # [ACTGGG] # 43 4602 # # BglII: # [AGATCT] # 715 996 2934 # # BinI*: # [GGATC] # 20 49 952 1053 1083 1488 # # BisI: # [GCNGC] # 58 224 225 245 415 533 # 730 2215 # # BlsI: # [GCNGC] # 58 225 225 245 415 533 # 730 2214 # # BmeT110I: # [CYCGRG] # 942 3703 # # BmgT120I: # [GGNCC] # 418 430 806 2991 # # BsaAI: # [YACGTR] # 486 847 859 2453 # # BsaXI: # [ACNNNNNCTCC] # 30 30 30 30 118 472 # 645 1311 1979 # # BseBI: # [CCWGG] # 23 261 558 934 954 1915 # # BseGI: # [GGATG] # 19 158 188 383 442 583 # 924 936 1012 # # BseMII: # [CTCAG] # 66 73 151 241 1969 2145 # # BseRI: # [GAGGAG] # 41 4604 # # BseSI: # [GKGCMC] # 1866 2779 # # BsgI: # [GTGCAG] # 250 542 696 1210 1947 # # BsiYI*: # [CCNNNNNNNGG] # 28 573 630 918 2496 # # BslFI: # [GGGAC] # 406 427 1068 2744 # # BsmAI: # [GTCTC] # 88 92 120 142 225 391 # 397 468 602 770 1350 # # BsmI: # [GAATGC] # 61 84 364 4136 # # Bsp1407I: # [TGTACA] # 246 333 676 3390 # # BspCNI: # [CTCAG] # 68 72 151 240 1971 2143 # # BspHI: # [TCATGA] # 434 1335 2876 # # BspLU11I*: # [ACATGT] # 152 4493 # # BspMI: # [ACCTGC] # 565 1170 1214 1696 # # BsrI: # [ACTGG] # 49 133 476 943 1012 2032 # # BssKI: # [CCNGG] # 21 261 558 934 956 1915 # # BssNAI: # [GTATAC] # 262 309 4074 # # BstEII: # [GGTNACC] # 711 1358 2576 # # BstKTI: # [GATC] # 13 15 16 62 62 66 # 110 148 150 156 165 368 # 379 504 623 641 1167 # # BstXI: # [CCANNNNNNTGG] # 842 3803 # # BtgZI: # [GCGATG] # 808 3837 # # BtrI: # [CACGTC] # 1023 3622 # # Cac8I: # [GCNNGC] # 136 1233 1564 1712 # # CfrI: # [YGGCCR] # 169 1262 3214 # # Csp6I: # [GTAC] # 9 38 52 79 105 119 # 156 195 249 259 260 294 # 339 402 633 676 780 # # CviAII: # [CATG] # 45 51 99 153 156 189 # 201 213 282 344 559 575 # 664 1114 # # CviJI: # [RGCY] # 5 16 16 23 26 26 # 30 38 45 49 49 64 # 72 73 74 81 83 88 # 98 98 101 103 106 123 # 125 131 141 166 181 196 # 204 237 251 293 311 418 # 504 # # CviRI*: # [TGCA] # 10 15 47 52 72 90 # 105 112 146 161 166 181 # 183 186 200 211 234 234 # 401 606 1233 # # DdeI: # [CTNAG] # 55 64 89 151 207 217 # 227 397 647 654 1937 # # DpnI: # [GATC] # 13 14 16 62 62 66 # 111 148 150 156 165 368 # 379 504 623 641 1167 # # DraII: # [RGGNCCY] # 1225 3420 # # DsaI*: # [CCRYGG] # 533 4112 # # Eam1105I: # [GACNNNNNGTC] # 12 1946 2687 # # EciI: # [GGCGGA] # 1105 3540 # # Eco31I: # [GGTCTC] # 995 1067 1141 1442 # # Eco57I: # [CTGAAG] # 78 798 807 1465 1497 # # Eco57MI: # [CTGRAG] # 78 129 149 678 798 1348 # 1465 # # EcoNI: # [CCTNNNNNAGG] # 1574 3071 # # EcoP15I: # [CAGCAG] # 1880 2765 # # EcoRII: # [CCWGG] # 21 261 558 934 956 1915 # # EcoRV: # [GATATC] # 163 164 4318 # # EcoT22I: # [ATGCAT] # 465 4180 # # Esp3I: # [CGTCTC] # 391 4254 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 0 0 # 0 0 32 32 32 32 # 76 248 314 906 2973 # # FatI: # [CATG] # 45 51 99 152 156 190 # 201 213 282 344 559 575 # 664 1114 # # FauI: # [CCCGC] # 228 1272 1429 1716 # # FnuDII*: # [CGCG] # 185 296 564 669 1181 1750 # # FokI: # [GGATG] # 39 138 188 383 442 596 # 923 924 1012 # # GlaI: # [GCGC] # 241 1044 1539 1821 # # GsuI: # [CTGGAG] # 827 1426 2392 # # HaeIII: # [GGCC] # 171 1260 3214 # # HgaI: # [GACGC] # 66 163 165 250 290 373 # 522 704 714 1398 # # HgiAI*: # [GWGCWC] # 246 846 3553 # # HgiCI*: # [GGYRCC] # 619 1312 2714 # # HgiJII*: # [GRGCYC] # 1283 3362 # # HhaI: # [GCGC] # 241 1043 1540 1821 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 0 0 # 0 0 0 0 0 0 # 1 26 32 32 32 32 # 32 32 32 32 32 258 # 289 370 373 439 470 497 # 596 1038 # # Hin4II*: # [CCTTC] # 32 39 70 268 575 579 # 1239 1843 # # Hin6I: # [GCGC] # 241 1045 1538 1821 # # HindII: # [GTYRAC] # 572 636 718 2719 # # HinfI: # [GANTC] # 12 12 41 63 65 109 # 114 130 153 174 188 217 # 219 220 289 502 2137 # # HpaII: # [CCGG] # 2130 2515 # # HphI: # [GGTGA] # 16 121 319 693 974 1008 # 1514 # # Hpy166II: # [GTNNAC] # 67 99 121 132 177 195 # 206 473 483 663 718 1311 # # Hpy178III*: # [TCNNGA] # 8 22 43 57 72 78 # 99 103 107 128 128 165 # 174 187 257 310 322 335 # 346 363 372 438 531 # # Hpy188I: # [TCNGA] # 10 10 11 12 40 41 # 44 59 76 95 97 99 # 99 111 124 150 152 186 # 225 229 254 269 279 299 # 326 357 395 596 # # Hpy99I: # [CGWCG] # 181 298 419 500 647 648 # 1952 # # KpnI: # [GGTACC] # 1935 2710 # # Ksp632I*: # [CTCTTC] # 497 533 539 3076 # # MaeI: # [CTAG] # 30 33 72 124 125 155 # 169 189 246 249 303 333 # 374 467 483 492 801 # # MaeII: # [ACGT] # 11 32 63 109 130 150 # 154 176 267 307 413 692 # 976 1165 # # MaeIII: # [GTNAC] # 6 10 15 31 46 50 # 63 78 129 133 161 180 # 184 223 241 259 311 401 # 439 565 1120 # # MboI: # [GATC] # 12 13 16 62 62 66 # 113 148 150 156 165 368 # 379 504 623 641 1167 # # MboII: # [GAAGA] # 17 18 25 27 29 41 # 46 46 62 62 65 85 # 215 258 260 265 335 347 # 348 352 450 521 771 # # MfeI: # [CAATTG] # 277 290 918 1110 2050 # # MluI: # [ACGCGT] # 298 1418 2929 # # MlyI: # [GAGTC] # 103 153 502 540 637 2710 # # MmeI: # [TCCRAC] # 44 98 102 346 441 464 # 1515 1635 # # MnlI: # [CCTC] # 1 3 19 31 63 68 # 69 91 230 258 276 278 # 356 413 414 443 477 524 # 631 # # MseI: # [TTAA] # 22 31 31 33 60 66 # 78 81 127 135 148 152 # 157 159 182 246 256 380 # 414 482 691 714 # # MslI: # [CAYNNNNRTG] # 157 472 834 915 2267 # # MstI*: # [TGCGCA] # 1539 3106 # # MwoI: # [GCNNNNNNNGC] # 232 618 878 1209 1708 # # NcoI: # [CCATGG] # 533 4112 # # NdeI: # [CATATG] # 164 1519 2962 # # NlaIII: # [CATG] # 45 51 99 156 156 186 # 201 213 282 344 559 575 # 664 1114 # # NlaIV: # [GGNNCC] # 158 621 901 1312 1653 # # NspBII*: # [CMGCKG] # 78 223 1123 3221 # # NspI: # [RCATGY] # 156 186 575 3728 # # OliI: # [CACNNNNGTG] # 2221 2424 # # PleI: # [GAGTC] # 103 153 502 540 637 2710 # # PpuMI: # [RGGWCCY] # 1225 3420 # # PshAI: # [GACNNNNGTC] # 2080 2565 # # PsrI: # [GAACNNNNNNTAC] # 32 32 32 245 563 806 # 2935 # # PstI: # [CTGCAG] # 202 1281 3162 # # RsaI: # [GTAC] # 9 38 52 79 105 119 # 156 196 249 259 260 293 # 339 402 633 676 780 # # SalI: # [GTCGAC] # 1928 2717 # # ScaI: # [AGTACT] # 259 1263 3123 # # ScrFI: # [CCNGG] # 23 261 558 934 954 1915 # # SduI: # [GDGCHC] # 246 437 846 1496 1620 # # SecI*: # [CCNNGG] # 21 46 512 934 956 2176 # # SetI: # [ASST] # 4 7 7 8 8 8 # 10 13 14 15 15 16 # 20 21 21 28 29 31 # 32 33 34 36 36 38 # 40 41 41 44 46 48 # 50 55 58 65 76 81 # 82 82 83 87 87 102 # 108 108 109 111 124 125 # 125 136 152 154 159 163 # 168 179 191 205 213 463 # # SfaNI: # [GCATC] # 168 417 480 635 791 990 # 1164 # # SfeI*: # [CTRYAG] # 31 86 109 198 317 478 # 989 1156 1281 # # SnaBI: # [TACGTA] # 1706 2939 # # SpeI: # [ACTAGT] # 1380 3265 # # SspI: # [AATATT] # 106 495 671 1612 1761 # # StyI: # [CCWWGG] # 533 4112 # # TaiI: # [ACGT] # 8 32 63 109 130 150 # 154 176 267 307 413 695 # 976 1165 # # TaqI: # [TCGA] # 57 68 68 90 109 130 # 132 135 136 157 192 198 # 203 278 309 345 391 411 # 525 711 # # TaqII: # [GACCGA] # 454 579 1369 2243 # # TatI: # [WGTACW] # 9 52 105 194 259 333 # 339 651 676 2027 # # TauI: # [GCSGC] # 58 1181 3406 # # TfiI: # [GAWTC] # 12 41 77 114 130 174 # 217 219 220 398 906 2137 # # TseI: # [GCWGC] # 223 225 415 533 1033 2216 # # TsoI: # [TARCCA] # 39 87 392 546 744 843 # 874 1120 # # Tsp45I: # [GTSAC] # 10 180 184 198 301 439 # 593 683 922 1135 # # Tsp4CI*: # [ACNGT] # 59 60 89 150 162 218 # 251 305 308 551 702 799 # 991 # # TspDTI: # [ATGAA] # 30 65 147 276 293 346 # 428 455 554 613 1438 # # TspEI: # [AATT] # 7 10 11 14 16 17 # 24 28 33 41 56 70 # 77 89 116 133 143 149 # 206 226 270 290 303 362 # 485 584 885 # # TspGWI: # [ACGGA] # 296 540 938 1076 1795 # # TspRI: # [CASTGNN] # 235 261 355 675 780 2339 # # TstI: # [CACNNNNNNTCC] # 32 2087 2526 # # Tth111I: # [GACNNNGTC] # 229 641 3775 # # VspI: # [ATTAAT] # 241 337 515 1737 1815 # # XbaI: # [TCTAGA] # 769 1938 1938 # # XcmI: # [CCANNNNNNNNNTGG] # 55 271 943 1046 1063 1267 # # XhoII: # [RGATCY] # 715 996 1364 1570 # # XmnI: # [GAANNNNTTC] # 59 4586 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 4645 # Reported_sequences: 1 # Reported_hitcount: 1012 #---------------------------------------