Restriction Fragments for YGR035W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGR035W-A on chromosome VII from coordinates 557565 to 557786.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26996.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 33 36 + MnlI CCTC 43 42 . . 40 44 + Tsp4CI* ACNGT 42 41 . . 61 64 + CviRI* TGCA 62 62 . . 62 72 + MwoI GCNNNNNNNGC 68 65 . . 62 72 + BstAPI GCANNNNNTGC 68 65 . . 82 86 + DdeI CTNAG 82 85 . . 91 95 + HphI GGTGA 103 102 . . 92 96 + Tsp45I GTSAC 91 96 . . 92 96 + MaeIII GTNAC 91 96 . . 89 94 - TaqII CACCCA 79 77 . . 95 100 + NspI RCATGY 99 95 . . 96 99 + FatI CATG 95 99 . . 96 99 + NlaIII CATG 99 95 . . 96 99 + CviAII CATG 96 98 . . 99 109 + MwoI GCNNNNNNNGC 105 102 . . 106 111 + NspBII* CMGCKG 108 108 . . 106 111 + SacII CCGCGG 109 107 . . 106 111 + DsaI* CCRYGG 106 110 . . 106 111 + SecI* CCNNGG 106 110 . . 106 109 + AciI CCGC 106 108 . . 107 110 + FnuDII* CGCG 108 108 . . 108 112 + TauI GCSGC 111 108 . . 108 112 + BlsI GCNGC 110 109 . . 108 112 + BisI GCNGC 109 110 . . 110 113 + CviJI RGCY 111 111 . . 108 111 - AciI CCGC 108 110 . . 117 122 + SmlI CTYRAG 117 121 . . 117 122 + AflII CTTAAG 117 121 . . 118 121 + MseI TTAA 118 120 . . 120 125 + HindIII AAGCTT 120 124 . . 121 124 + SetI ASST 124 120 . . 121 124 + CviJI RGCY 122 122 . . 121 124 + AluI AGCT 122 122 . . 135 139 + BccI CCATC 143 144 . . 147 152 + Hpy178III* TCNNGA 148 150 . . 153 156 + SetI ASST 156 152 . . 153 156 + CviJI RGCY 154 154 . . 153 156 + AluI AGCT 154 154 . . 157 162 + NspBII* CMGCKG 159 159 . . 157 160 + AciI CCGC 157 159 . . 168 173 + DsaI* CCRYGG 168 172 . . 168 173 + SecI* CCNNGG 168 172 . . 167 171 - TspGWI ACGGA 157 155 . . 190 194 - BceAI ACGGC 175 177 . . 206 211 + Hpy178III* TCNNGA 207 209 . . 207 212 + SmlI CTYRAG 207 211 . . 207 212 + XhoI CTCGAG 207 211 . . 203 207 - MboII GAAGA 195 194 . . 207 212 + BmeT110I CYCGRG 208 210 . . 207 212 + AvaI CYCGRG 207 211 . . 208 211 + TaqI TCGA 208 210 . . 208 213 + Hpy178III* TCNNGA 209 211 . . #--------------------------------------- # # # AciI: # [CCGC] # 2 49 65 106 # # AflII: # [CTTAAG] # 105 117 # # AluI: # [AGCT] # 32 68 122 # # AvaI: # [CYCGRG] # 15 207 # # BccI: # [CCATC] # 79 143 # # BceAI: # [ACGGC] # 47 175 # # BisI: # [GCNGC] # 109 113 # # BlsI: # [GCNGC] # 110 112 # # BmeT110I: # [CYCGRG] # 14 208 # # BstAPI: # [GCANNNNNTGC] # 68 154 # # CviAII: # [CATG] # 96 126 # # CviJI: # [RGCY] # 11 32 68 111 # # CviRI*: # [TGCA] # 62 160 # # DdeI: # [CTNAG] # 82 140 # # DsaI*: # [CCRYGG] # 54 62 106 # # FatI: # [CATG] # 95 127 # # FnuDII*: # [CGCG] # 108 114 # # HindIII: # [AAGCTT] # 102 120 # # HphI: # [GGTGA] # 103 119 # # Hpy178III*: # [TCNNGA] # 2 13 59 148 # # MaeIII: # [GTNAC] # 91 131 # # MboII: # [GAAGA] # 27 195 # # MnlI: # [CCTC] # 43 179 # # MseI: # [TTAA] # 104 118 # # MwoI: # [GCNNNNNNNGC] # 37 68 117 # # NlaIII: # [CATG] # 99 123 # # NspBII*: # [CMGCKG] # 51 63 108 # # NspI: # [RCATGY] # 99 123 # # SacII: # [CCGCGG] # 109 113 # # SecI*: # [CCNNGG] # 54 62 106 # # SetI: # [ASST] # 32 66 124 # # SmlI: # [CTYRAG] # 15 90 117 # # TaqI: # [TCGA] # 14 208 # # TaqII: # [CACCCA] # 79 143 # # TauI: # [GCSGC] # 111 111 # # Tsp45I: # [GTSAC] # 91 131 # # Tsp4CI*: # [ACNGT] # 42 180 # # TspGWI: # [ACGGA] # 65 157 # # XhoI: # [CTCGAG] # 15 207 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 222 # Reported_sequences: 1 # Reported_hitcount: 52 #---------------------------------------