Restriction Fragments for MSB2/YGR014W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

MSB2/YGR014W on chromosome VII from coordinates 516943 to 520863.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.32222.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 20 24 + BsmAI GTCTC 25 29 . . 28 31 + TaqI TCGA 28 30 . . 39 42 + TspEI AATT 38 42 . . 54 57 + CviJI RGCY 55 55 . . 54 57 + HaeIII GGCC 55 55 . . 54 58 + BmgT120I GGNCC 55 56 . . 54 58 + AsuI* GGNCC 54 57 . . 55 62 + SrfI GCCCGGGC 58 58 . . 56 61 + SmaI CCCGGG 58 58 . . 56 61 + Cfr9I CCCGGG 56 60 . . 56 60 + BssKI CCNGG 55 60 . . 56 61 + SecI* CCNNGG 56 60 . . 56 61 + BmeT110I CYCGRG 57 59 . . 56 60 + ScrFI CCNGG 57 58 . . 56 60 + CauII* CCSGG 57 58 . . 56 61 + AvaI CYCGRG 56 60 . . 57 61 + BssKI CCNGG 56 61 . . 57 60 + HpaII CCGG 57 59 . . 57 61 + ScrFI CCNGG 58 59 . . 57 61 + CauII* CCSGG 58 59 . . 59 63 + BmgT120I GGNCC 60 61 . . 59 63 + AsuI* GGNCC 59 62 . . 60 63 + CviJI RGCY 61 61 . . 60 63 + HaeIII GGCC 61 61 . . 61 66 + Cac8I GCNNGC 63 63 . . 64 67 + CviJI RGCY 65 65 . . 68 72 + Hin4II* CCTTC 78 77 . . 71 74 + TaqI TCGA 71 73 . . 86 91 + BsrDI GCAATG 93 91 . . 102 105 + SetI ASST 105 101 . . 102 105 + CviJI RGCY 103 103 . . 102 105 + AluI AGCT 103 103 . . 104 108 + DdeI CTNAG 104 107 . . 104 108 + BspCNI CTCAG 117 115 . . 104 108 + BseMII CTCAG 118 116 . . 105 109 + Hpy188I TCNGA 107 106 . . 109 112 + CviJI RGCY 110 110 . . 120 124 + PleI GAGTC 128 129 . . 120 124 + MlyI GAGTC 129 129 . . 120 124 + HinfI GANTC 120 123 . . 126 129 + SetI ASST 129 125 . . 120 130 - Hin4I GAYNNNNNVTC 143 138 111 106 140 144 - HphI GGTGA 132 131 . . 146 150 + TspDTI ATGAA 161 159 . . 147 158 + BdaI TGANNNNNNTCA 136 134 170 168 149 154 + HindIII AAGCTT 149 153 . . 150 153 + SetI ASST 153 149 . . 150 153 + CviJI RGCY 151 151 . . 150 153 + AluI AGCT 151 151 . . 155 159 + DdeI CTNAG 155 158 . . 155 159 + BspCNI CTCAG 168 166 . . 155 159 + BseMII CTCAG 169 167 . . 156 161 + Hpy178III* TCNNGA 157 159 . . 147 158 - BdaI TGANNNNNNTCA 170 168 136 134 167 181 + XcmI CCANNNNNNNNNTGG 174 173 . . 170 181 + BstXI CCANNNNNNTGG 177 173 . . 172 175 + SetI ASST 175 171 . . 173 176 + MnlI CCTC 183 182 . . 181 185 + MaeIII GTNAC 180 185 . . 186 189 + CviJI RGCY 187 187 . . 194 199 + SmlI CTYRAG 194 198 . . 198 201 + SetI ASST 201 197 . . 194 199 - Bce83I* CTTGAG 179 177 . . 203 207 - TspDTI ATGAA 193 191 . . 210 221 + AlfI GCANNNNNNTGC 199 197 233 231 213 217 - BsrI ACTGG 213 211 . . 219 222 + CviRI* TGCA 220 220 . . 210 221 - AlfI GCANNNNNNTGC 233 231 199 197 243 248 + Ksp632I* CTCTTC 249 252 . . 244 248 - MboII GAAGA 236 235 . . 249 252 + MnlI CCTC 259 258 . . 253 264 + BstXI CCANNNNNNTGG 260 256 . . 253 257 + BccI CCATC 261 262 . . 268 278 + Hin4I GAYNNNNNVTC 259 254 291 286 271 276 + Cac8I GCNNGC 273 273 . . 268 272 - SfaNI GCATC 258 262 . . 273 278 + SacI GAGCTC 277 273 . . 273 278 + Ecl136II GAGCTC 275 275 . . 273 278 + SduI GDGCHC 277 273 . . 273 278 + HgiJII* GRGCYC 277 273 . . 273 278 + HgiAI* GWGCWC 277 273 . . 274 277 + SetI ASST 277 273 . . 274 277 + CviJI RGCY 275 275 . . 274 277 + AluI AGCT 275 275 . . 288 299 + DrdI GACNNNNNNGTC 294 292 . . 299 304 + NdeI CATATG 300 302 . . 309 314 + Hpy178III* TCNNGA 310 312 . . 305 309 - BsrI ACTGG 305 303 . . 313 317 + TfiI GAWTC 313 316 . . 313 317 + HinfI GANTC 313 316 . . 316 321 + MmeI TCCRAC 341 339 . . 316 320 + Hpy188I TCNGA 318 317 . . 329 332 + MseI TTAA 329 331 . . 325 329 - BceAI ACGGC 310 312 . . 347 350 + Hin6I GCGC 347 349 . . 347 350 + GlaI GCGC 348 348 . . 347 350 + HhaI GCGC 349 347 . . 348 351 + FnuDII* CGCG 349 349 . . 351 356 + Hpy166II GTNNAC 353 353 . . 351 356 + AccI GTMKAC 352 354 . . 349 353 - HgaI GACGC 338 343 . . 367 371 + BccI CCATC 375 376 . . 377 380 + Csp6I GTAC 377 379 . . 377 380 + RsaI GTAC 378 378 . . 374 378 - BsrI ACTGG 374 372 . . 385 388 + SetI ASST 388 384 . . 385 388 + CviJI RGCY 386 386 . . 385 388 + AluI AGCT 386 386 . . 394 397 + Hin6I GCGC 394 396 . . 394 397 + GlaI GCGC 395 395 . . 394 397 + HhaI GCGC 396 394 . . 400 403 + SetI ASST 403 399 . . 401 405 + Hin4II* CCTTC 411 410 . . 406 411 + TatI WGTACW 406 410 . . 402 407 - Eco57MI CTGRAG 387 385 . . 407 410 + Csp6I GTAC 407 409 . . 407 410 + RsaI GTAC 408 408 . . 402 407 - Eco57I CTGAAG 387 385 . . 413 418 + McrI* CGRYCG 416 414 . . 413 418 + XmaIII* CGGCCG 413 417 . . 413 418 + CfrI YGGCCR 413 417 . . 414 417 + CviJI RGCY 415 415 . . 414 417 + HaeIII GGCC 415 415 . . 415 425 + MwoI GCNNNNNNNGC 421 418 . . 417 428 + BcgI CGANNNNNNTGC 406 404 440 438 424 428 + BlsI GCNGC 426 425 . . 424 428 + BisI GCNGC 425 426 . . 424 428 + TseI GCWGC 424 427 . . 424 428 - BbvI GCAGC 411 415 . . 431 434 + MaeI CTAG 431 433 . . 441 444 + CviRI* TGCA 442 442 . . 442 446 + BlsI GCNGC 444 443 . . 442 446 + BisI GCNGC 443 444 . . 442 446 + BbvI GCAGC 454 458 . . 442 446 + TseI GCWGC 442 445 . . 443 451 + AlwNI CAGNNNCTG 448 445 . . 444 447 + SetI ASST 447 443 . . 444 447 + CviJI RGCY 445 445 . . 444 447 + AluI AGCT 445 445 . . 448 452 + Hpy188I TCNGA 450 449 . . 454 458 + BsmAI GTCTC 459 463 . . 459 465 + TspRI CASTGNN 465 456 . . 459 464 - BtsI GCAGTG 458 456 . . 465 471 + TspRI CASTGNN 471 462 . . 464 468 - BsrI ACTGG 464 462 . . 490 493 + TspEI AATT 489 493 . . 515 519 + MaeIII GTNAC 514 519 . . 523 527 - BsmAI GTCTC 517 521 . . 534 544 + BplI GAGNNNNNCTC 525 520 557 552 536 541 + Hpy166II GTNNAC 538 538 . . 536 541 + SduI GDGCHC 540 536 . . 536 541 + BseSI GKGCMC 540 536 . . 536 541 + ApaLI GTGCAC 536 540 . . 536 541 + HgiAI* GWGCWC 540 536 . . 537 540 + CviRI* TGCA 538 538 . . 540 543 + SetI ASST 543 539 . . 541 544 + MnlI CCTC 551 550 . . 534 544 - BplI GAGNNNNNCTC 557 552 525 520 550 554 + Hpy188I TCNGA 552 551 . . 571 576 + SpeI ACTAGT 571 575 . . 572 575 + MaeI CTAG 572 574 . . 575 578 + Csp6I GTAC 575 577 . . 575 578 + RsaI GTAC 576 576 . . 584 587 + AciI CCGC 584 586 . . 586 590 + BlsI GCNGC 588 587 . . 586 590 + BisI GCNGC 587 588 . . 586 590 + BbvI GCAGC 598 602 . . 586 590 + TseI GCWGC 586 589 . . 587 592 + PvuII CAGCTG 589 589 . . 587 592 + NspBII* CMGCKG 589 589 . . 588 591 + SetI ASST 591 587 . . 588 591 + CviJI RGCY 589 589 . . 588 591 + AluI AGCT 589 589 . . 596 599 + TaiI ACGT 599 595 . . 596 599 + SetI ASST 599 595 . . 596 599 + MaeII ACGT 596 598 . . 626 629 + MnlI CCTC 636 635 . . 649 653 + Hpy188I TCNGA 651 650 . . 670 673 + TaqI TCGA 670 672 . . 674 679 - EcoP15I CAGCAG 646 648 . . 679 684 + NlaIV GGNNCC 681 681 . . 679 684 + HgiCI* GGYRCC 679 683 . . 684 693 + MslI CAYNNNNRTG 688 688 . . 687 692 + BetI* WCCGGW 687 691 . . 688 691 + HpaII CCGG 688 690 . . 692 695 + CviRI* TGCA 693 693 . . 701 706 + EcoP15I CAGCAG 731 733 . . 719 722 + MaeI CTAG 719 721 . . 725 730 + ApoI RAATTY 725 729 . . 726 729 + TspEI AATT 725 729 . . 732 737 + TatI WGTACW 732 736 . . 733 736 + Csp6I GTAC 733 735 . . 733 736 + RsaI GTAC 734 734 . . 744 749 + EciI GGCGGA 760 758 . . 745 748 - AciI CCGC 745 747 . . 757 760 + CviJI RGCY 758 758 . . 761 764 + MaeI CTAG 761 763 . . 755 767 - PsrI GAACNNNNNNTAC 779 774 747 742 768 773 + MmeI TCCRAC 793 791 . . 794 799 + PvuII CAGCTG 796 796 . . 794 799 + NspBII* CMGCKG 796 796 . . 795 798 + SetI ASST 798 794 . . 795 798 + CviJI RGCY 796 796 . . 795 798 + AluI AGCT 796 796 . . 796 800 + BlsI GCNGC 798 797 . . 796 800 + BisI GCNGC 797 798 . . 796 800 + TseI GCWGC 796 799 . . 796 800 - BbvI GCAGC 783 787 . . 803 809 + TspRI CASTGNN 809 800 . . 806 809 + CviRI* TGCA 807 807 . . 803 808 - BtsI GCAGTG 802 800 . . 817 821 + TfiI GAWTC 817 820 . . 817 821 + HinfI GANTC 817 820 . . 834 840 + TspRI CASTGNN 840 831 . . 837 840 + CviRI* TGCA 838 838 . . 838 842 + SfaNI GCATC 847 851 . . 838 848 + MwoI GCNNNNNNNGC 844 841 . . 834 839 - BtsI GCAGTG 833 831 . . 842 847 + EcoP15I CAGCAG 872 874 . . 844 848 + BlsI GCNGC 846 845 . . 844 848 + BisI GCNGC 845 846 . . 844 848 + BbvI GCAGC 856 860 . . 844 848 + TseI GCWGC 844 847 . . 846 849 + SetI ASST 849 845 . . 846 849 + CviJI RGCY 847 847 . . 846 849 + AluI AGCT 847 847 . . 853 857 - BsrI ACTGG 853 851 . . 869 872 + TaiI ACGT 872 868 . . 869 872 + SetI ASST 872 868 . . 869 872 + MaeII ACGT 869 871 . . 877 880 + CviJI RGCY 878 878 . . 874 878 - BccI CCATC 868 869 . . 879 884 + SmlI CTYRAG 879 883 . . 883 888 + HaeII RGCGCY 887 883 . . 884 887 + Hin6I GCGC 884 886 . . 884 887 + GlaI GCGC 885 885 . . 879 884 - Bce83I* CTTGAG 864 862 . . 884 887 + HhaI GCGC 886 884 . . 887 890 + MnlI CCTC 897 896 . . 891 894 + SetI ASST 894 890 . . 893 898 + SfeI* CTRYAG 893 897 . . 889 893 - HphI GGTGA 881 880 . . 903 906 + FatI CATG 902 906 . . 903 906 + NlaIII CATG 906 902 . . 903 906 + CviAII CATG 903 905 . . 907 912 + HaeII RGCGCY 911 907 . . 908 911 + Hin6I GCGC 908 910 . . 908 911 + GlaI GCGC 909 909 . . 908 911 + HhaI GCGC 910 908 . . 910 914 + TauI GCSGC 913 910 . . 910 914 + BlsI GCNGC 912 911 . . 910 914 + BisI GCNGC 911 912 . . 911 914 + AciI CCGC 911 913 . . 924 927 + SetI ASST 927 923 . . 924 927 + CviJI RGCY 925 925 . . 924 927 + AluI AGCT 925 925 . . 925 930 + Cac8I GCNNGC 927 927 . . 925 930 + BmtI GCTAGC 929 925 . . 925 930 + NheI GCTAGC 925 929 . . 926 929 + MaeI CTAG 926 928 . . 928 931 + SetI ASST 931 927 . . 928 931 + CviJI RGCY 929 929 . . 928 931 + AluI AGCT 929 929 . . 929 939 + MwoI GCNNNNNNNGC 935 932 . . 930 935 + SmlI CTYRAG 930 934 . . 930 935 - Bce83I* CTTGAG 915 913 . . 935 939 + BlsI GCNGC 937 936 . . 935 939 + BisI GCNGC 936 937 . . 935 939 + BbvI GCAGC 947 951 . . 935 939 + TseI GCWGC 935 938 . . 939 950 + TstI CACNNNNNNTCC 930 925 962 957 948 953 + MmeI TCCRAC 973 971 . . 958 962 + Hpy188I TCNGA 960 959 . . 964 967 + SetI ASST 967 963 . . 972 977 + Hpy166II GTNNAC 974 974 . . 972 977 + HpaI GTTAAC 974 974 . . 972 977 + HindII GTYRAC 974 974 . . 973 976 + MseI TTAA 973 975 . . 980 985 + SfeI* CTRYAG 980 984 . . 994 999 + NlaIV GGNNCC 996 996 . . 991 995 - BccI CCATC 985 986 . . 997 1001 + Hpy188I TCNGA 999 998 . . 1000 1010 + Hin4I GAYNNNNNVTC 991 986 1023 1018 1005 1009 - TspDTI ATGAA 995 993 . . 1000 1010 - Hin4I GAYNNNNNVTC 1023 1018 991 986 1012 1016 + Tsp4CI* ACNGT 1014 1013 . . 1013 1019 + TspRI CASTGNN 1019 1010 . . 1019 1022 + Hin6I GCGC 1019 1021 . . 1019 1022 + GlaI GCGC 1020 1020 . . 1019 1022 + HhaI GCGC 1021 1019 . . 1032 1035 + CviJI RGCY 1033 1033 . . 1032 1037 + NlaIV GGNNCC 1034 1034 . . 1021 1032 - BcgI CGANNNNNNTGC 1044 1042 1010 1008 1051 1056 + TatI WGTACW 1051 1055 . . 1051 1056 + ScaI AGTACT 1053 1053 . . 1052 1055 + Csp6I GTAC 1052 1054 . . 1052 1055 + RsaI GTAC 1053 1053 . . 1057 1062 + MmeI TCCRAC 1082 1080 . . 1061 1065 + Tsp4CI* ACNGT 1063 1062 . . 1070 1080 + MwoI GCNNNNNNNGC 1076 1073 . . 1078 1081 + CviJI RGCY 1079 1079 . . 1081 1085 + BccI CCATC 1089 1090 . . 1087 1099 - PsrI GAACNNNNNNTAC 1111 1106 1079 1074 1099 1103 + BccI CCATC 1107 1108 . . 1112 1115 + MnlI CCTC 1122 1121 . . 1113 1117 + DdeI CTNAG 1113 1116 . . 1113 1117 + BspCNI CTCAG 1126 1124 . . 1113 1117 + BseMII CTCAG 1127 1125 . . 1114 1118 + Hpy188I TCNGA 1116 1115 . . 1118 1121 + TaiI ACGT 1121 1117 . . 1118 1121 + SetI ASST 1121 1117 . . 1118 1121 + MaeII ACGT 1118 1120 . . 1123 1128 + Cac8I GCNNGC 1125 1125 . . 1123 1128 + BmtI GCTAGC 1127 1123 . . 1123 1128 + NheI GCTAGC 1123 1127 . . 1124 1127 + MaeI CTAG 1124 1126 . . 1126 1129 + SetI ASST 1129 1125 . . 1126 1129 + CviJI RGCY 1127 1127 . . 1126 1129 + AluI AGCT 1127 1127 . . 1132 1137 + TatI WGTACW 1132 1136 . . 1132 1137 + ScaI AGTACT 1134 1134 . . 1128 1133 - Eco57MI CTGRAG 1113 1111 . . 1133 1136 + Csp6I GTAC 1133 1135 . . 1128 1133 - GsuI CTGGAG 1113 1111 . . 1133 1136 + RsaI GTAC 1134 1134 . . 1130 1134 - BsrI ACTGG 1130 1128 . . 1137 1140 + CviRI* TGCA 1138 1138 . . 1152 1156 - TspDTI ATGAA 1142 1140 . . 1154 1158 - FokI GGATG 1140 1144 . . 1154 1158 - BseGI GGATG 1153 1151 . . 1162 1166 + Hpy188I TCNGA 1164 1163 . . 1174 1177 + AciI CCGC 1174 1176 . . 1180 1185 + SpeI ACTAGT 1180 1184 . . 1181 1184 + MaeI CTAG 1181 1183 . . 1184 1187 + Csp6I GTAC 1184 1186 . . 1184 1187 + RsaI GTAC 1185 1185 . . 1186 1189 + SetI ASST 1189 1185 . . 1187 1190 + MnlI CCTC 1197 1196 . . 1187 1193 + SauI* CCTNAGG 1188 1191 . . 1188 1192 + DdeI CTNAG 1188 1191 . . 1188 1192 + BspCNI CTCAG 1201 1199 . . 1188 1192 + BseMII CTCAG 1202 1200 . . 1210 1213 + Hin6I GCGC 1210 1212 . . 1210 1213 + GlaI GCGC 1211 1211 . . 1210 1220 + MwoI GCNNNNNNNGC 1216 1213 . . 1210 1213 + HhaI GCGC 1212 1210 . . 1217 1220 + AciI CCGC 1217 1219 . . 1219 1223 + SfaNI GCATC 1228 1232 . . 1227 1230 + MnlI CCTC 1237 1236 . . 1250 1253 + SetI ASST 1253 1249 . . 1249 1252 - MnlI CCTC 1242 1241 . . 1258 1263 + NlaIV GGNNCC 1260 1260 . . 1258 1263 + HgiCI* GGYRCC 1258 1262 . . 1263 1269 + TspRI CASTGNN 1269 1260 . . 1262 1266 - BsrI ACTGG 1262 1260 . . 1268 1275 + PspXI VCTCGAGB 1269 1273 . . 1268 1271 + MnlI CCTC 1278 1277 . . 1269 1274 + SmlI CTYRAG 1269 1273 . . 1269 1274 + XhoI CTCGAG 1269 1273 . . 1269 1274 + BmeT110I CYCGRG 1270 1272 . . 1269 1274 + AvaI CYCGRG 1269 1273 . . 1270 1273 + TaqI TCGA 1270 1272 . . 1272 1277 + SduI GDGCHC 1276 1272 . . 1272 1277 + HgiAI* GWGCWC 1276 1272 . . 1284 1288 - TspDTI ATGAA 1274 1272 . . 1306 1311 + SfeI* CTRYAG 1306 1310 . . 1308 1312 + Tsp4CI* ACNGT 1310 1309 . . 1311 1316 + Hpy166II GTNNAC 1313 1313 . . 1311 1316 + AccI GTMKAC 1312 1314 . . 1327 1330 + SetI ASST 1330 1326 . . 1327 1330 + CviJI RGCY 1328 1328 . . 1327 1330 + AluI AGCT 1328 1328 . . 1329 1334 + SmlI CTYRAG 1329 1333 . . 1329 1334 - Bce83I* CTTGAG 1314 1312 . . 1338 1341 + SetI ASST 1341 1337 . . 1338 1341 + CviJI RGCY 1339 1339 . . 1338 1341 + AluI AGCT 1339 1339 . . 1339 1344 + Cac8I GCNNGC 1341 1341 . . 1339 1344 + BmtI GCTAGC 1343 1339 . . 1339 1344 + NheI GCTAGC 1339 1343 . . 1340 1343 + MaeI CTAG 1340 1342 . . 1342 1345 + SetI ASST 1345 1341 . . 1342 1345 + CviJI RGCY 1343 1343 . . 1342 1345 + AluI AGCT 1343 1343 . . 1346 1349 + MnlI CCTC 1356 1355 . . 1344 1349 - BseRI GAGGAG 1335 1333 . . 1352 1356 + Hpy99I CGWCG 1356 1351 . . 1354 1357 + TaqI TCGA 1354 1356 . . 1351 1355 - HgaI GACGC 1340 1345 . . 1360 1364 + Hpy188I TCNGA 1362 1361 . . 1364 1367 + SetI ASST 1367 1363 . . 1378 1383 + AcyI GRCGYC 1379 1381 . . 1380 1385 + Esp3I CGTCTC 1386 1390 . . 1381 1385 + BsmAI GTCTC 1386 1390 . . 1379 1383 - HgaI GACGC 1368 1373 . . 1387 1392 + TatI WGTACW 1387 1391 . . 1383 1388 - Eco57MI CTGRAG 1368 1366 . . 1388 1391 + Csp6I GTAC 1388 1390 . . 1383 1388 - GsuI CTGGAG 1368 1366 . . 1388 1391 + RsaI GTAC 1389 1389 . . 1385 1389 - BsrI ACTGG 1385 1383 . . 1393 1398 + Cac8I GCNNGC 1395 1395 . . 1402 1407 + NspBII* CMGCKG 1404 1404 . . 1402 1405 + AciI CCGC 1402 1404 . . 1413 1416 + CviJI RGCY 1414 1414 . . 1415 1418 + MnlI CCTC 1425 1424 . . 1416 1420 + DdeI CTNAG 1416 1419 . . 1416 1420 + BspCNI CTCAG 1429 1427 . . 1416 1420 + BseMII CTCAG 1430 1428 . . 1417 1421 + Hpy188I TCNGA 1419 1418 . . 1429 1434 + TatI WGTACW 1429 1433 . . 1429 1434 + ScaI AGTACT 1431 1431 . . 1430 1433 + Csp6I GTAC 1430 1432 . . 1430 1433 + RsaI GTAC 1431 1431 . . 1437 1442 + EcoP15I CAGCAG 1467 1469 . . 1439 1449 + MwoI GCNNNNNNNGC 1445 1442 . . 1448 1458 + MwoI GCNNNNNNNGC 1454 1451 . . 1456 1459 + CviJI RGCY 1457 1457 . . 1459 1463 + Hin4II* CCTTC 1469 1468 . . 1465 1477 - PsrI GAACNNNNNNTAC 1489 1484 1457 1452 1477 1481 + BccI CCATC 1485 1486 . . 1490 1493 + MnlI CCTC 1500 1499 . . 1491 1495 + DdeI CTNAG 1491 1494 . . 1491 1495 + BspCNI CTCAG 1504 1502 . . 1491 1495 + BseMII CTCAG 1505 1503 . . 1492 1496 + Hpy188I TCNGA 1494 1493 . . 1501 1506 + Cac8I GCNNGC 1503 1503 . . 1501 1506 + BmtI GCTAGC 1505 1501 . . 1501 1506 + NheI GCTAGC 1501 1505 . . 1502 1505 + MaeI CTAG 1502 1504 . . 1506 1517 + TstI CACNNNNNNTCC 1497 1492 1529 1524 1507 1517 + BsaXI ACNNNNNCTCC 1497 1494 1527 1524 1509 1515 + TspRI CASTGNN 1515 1506 . . 1511 1516 + SduI GDGCHC 1515 1511 . . 1511 1516 + HgiAI* GWGCWC 1515 1511 . . 1508 1512 - BsrI ACTGG 1508 1506 . . 1530 1534 - TspDTI ATGAA 1520 1518 . . 1532 1536 - FokI GGATG 1518 1522 . . 1532 1536 - BseGI GGATG 1531 1529 . . 1550 1556 + TspRI CASTGNN 1556 1547 . . 1553 1556 + CviRI* TGCA 1554 1554 . . 1554 1559 + Cac8I GCNNGC 1556 1556 . . 1550 1555 - BtsI GCAGTG 1549 1547 . . 1560 1563 + SetI ASST 1563 1559 . . 1558 1563 - BspMI ACCTGC 1549 1553 . . 1564 1567 + CviJI RGCY 1565 1565 . . 1558 1564 - AarI CACCTGC 1549 1553 . . 1568 1571 + MaeI CTAG 1568 1570 . . 1572 1575 + FatI CATG 1571 1575 . . 1572 1575 + NlaIII CATG 1575 1571 . . 1572 1575 + CviAII CATG 1572 1574 . . 1583 1586 + MnlI CCTC 1593 1592 . . 1584 1589 + Ksp632I* CTCTTC 1590 1593 . . 1585 1589 - MboII GAAGA 1577 1576 . . 1587 1591 - TspDTI ATGAA 1577 1575 . . 1595 1600 + SfeI* CTRYAG 1595 1599 . . 1612 1617 + TatI WGTACW 1612 1616 . . 1612 1617 + ScaI AGTACT 1614 1614 . . 1613 1616 + Csp6I GTAC 1613 1615 . . 1613 1616 + RsaI GTAC 1614 1614 . . 1615 1619 + BsrI ACTGG 1620 1618 . . 1624 1628 + Hpy188I TCNGA 1626 1625 . . 1613 1625 - PsrI GAACNNNNNNTAC 1637 1632 1605 1600 1625 1629 + DdeI CTNAG 1625 1628 . . 1625 1629 - BspCNI CTCAG 1617 1615 . . 1625 1629 - BseMII CTCAG 1616 1614 . . 1630 1634 + TfiI GAWTC 1630 1633 . . 1627 1630 - MnlI CCTC 1620 1619 . . 1630 1634 + HinfI GANTC 1630 1633 . . 1634 1639 + NspBII* CMGCKG 1636 1636 . . 1634 1637 + AciI CCGC 1634 1636 . . 1636 1640 + BlsI GCNGC 1638 1637 . . 1636 1640 + BisI GCNGC 1637 1638 . . 1636 1640 + TseI GCWGC 1636 1639 . . 1638 1641 + CviRI* TGCA 1639 1639 . . 1639 1643 + SfaNI GCATC 1648 1652 . . 1639 1649 + MwoI GCNNNNNNNGC 1645 1642 . . 1636 1640 - BbvI GCAGC 1623 1627 . . 1643 1648 + SfeI* CTRYAG 1643 1647 . . 1646 1654 + AlwNI CAGNNNCTG 1651 1648 . . 1647 1650 + SetI ASST 1650 1646 . . 1647 1650 + CviJI RGCY 1648 1648 . . 1647 1650 + AluI AGCT 1648 1648 . . 1653 1656 + CviRI* TGCA 1654 1654 . . 1658 1664 + TspRI CASTGNN 1664 1655 . . 1659 1663 + Tsp4CI* ACNGT 1661 1660 . . 1665 1670 + SduI GDGCHC 1669 1665 . . 1654 1665 - BcgI CGANNNNNNTGC 1677 1675 1643 1641 1665 1670 + HgiJII* GRGCYC 1669 1665 . . 1666 1669 + CviJI RGCY 1667 1667 . . 1666 1671 + NlaIV GGNNCC 1668 1668 . . 1670 1673 + MnlI CCTC 1680 1679 . . 1668 1673 - BseRI GAGGAG 1659 1657 . . 1674 1679 + Hpy166II GTNNAC 1676 1676 . . 1674 1679 + AccI GTMKAC 1675 1677 . . 1692 1697 + ApoI RAATTY 1692 1696 . . 1693 1696 + TspEI AATT 1692 1696 . . 1697 1700 + CviRI* TGCA 1698 1698 . . 1698 1708 + MwoI GCNNNNNNNGC 1704 1701 . . 1706 1709 + CviJI RGCY 1707 1707 . . 1708 1711 + MnlI CCTC 1718 1717 . . 1711 1715 + BccI CCATC 1719 1720 . . 1712 1716 - FokI GGATG 1698 1702 . . 1712 1716 - BseGI GGATG 1711 1709 . . 1732 1736 + Hpy188I TCNGA 1734 1733 . . 1735 1739 + PleI GAGTC 1743 1744 . . 1735 1739 + MlyI GAGTC 1744 1744 . . 1735 1739 + HinfI GANTC 1735 1738 . . 1737 1741 + BsmAI GTCTC 1742 1746 . . 1739 1744 + SmlI CTYRAG 1739 1743 . . 1743 1746 + SetI ASST 1746 1742 . . 1743 1746 + CviJI RGCY 1744 1744 . . 1743 1746 + AluI AGCT 1744 1744 . . 1739 1744 - Bce83I* CTTGAG 1724 1722 . . 1757 1762 + PvuII CAGCTG 1759 1759 . . 1757 1762 + NspBII* CMGCKG 1759 1759 . . 1758 1761 + SetI ASST 1761 1757 . . 1758 1761 + CviJI RGCY 1759 1759 . . 1758 1761 + AluI AGCT 1759 1759 . . 1754 1759 - Eco57MI CTGRAG 1739 1737 . . 1759 1769 + MwoI GCNNNNNNNGC 1765 1762 . . 1754 1759 - Eco57I CTGAAG 1739 1737 . . 1764 1769 + Cac8I GCNNGC 1766 1766 . . 1764 1769 + BmtI GCTAGC 1768 1764 . . 1764 1769 + NheI GCTAGC 1764 1768 . . 1765 1768 + MaeI CTAG 1765 1767 . . 1767 1770 + SetI ASST 1770 1766 . . 1767 1770 + CviJI RGCY 1768 1768 . . 1767 1770 + AluI AGCT 1768 1768 . . 1768 1773 + Cac8I GCNNGC 1770 1770 . . 1768 1773 + BmtI GCTAGC 1772 1768 . . 1768 1773 + NheI GCTAGC 1768 1772 . . 1769 1772 + MaeI CTAG 1769 1771 . . 1792 1796 - HphI GGTGA 1784 1783 . . 1803 1809 + TspRI CASTGNN 1809 1800 . . 1805 1810 + PstI CTGCAG 1809 1805 . . 1805 1810 + SfeI* CTRYAG 1805 1809 . . 1806 1809 + CviRI* TGCA 1807 1807 . . 1807 1817 + MwoI GCNNNNNNNGC 1813 1810 . . 1807 1817 + BstAPI GCANNNNNTGC 1813 1810 . . 1803 1808 - BtsI GCAGTG 1802 1800 . . 1809 1812 + SetI ASST 1812 1808 . . 1807 1812 - BspMI ACCTGC 1798 1802 . . 1807 1813 - AarI CACCTGC 1798 1802 . . 1815 1818 + CviRI* TGCA 1816 1816 . . 1816 1820 + SfaNI GCATC 1825 1829 . . 1820 1825 + SfeI* CTRYAG 1820 1824 . . 1826 1831 + StuI AGGCCT 1828 1828 . . 1827 1830 + CviJI RGCY 1828 1828 . . 1827 1830 + HaeIII GGCC 1828 1828 . . 1825 1828 - MnlI CCTC 1818 1817 . . 1829 1832 + MnlI CCTC 1839 1838 . . 1825 1835 - Hin4I GAYNNNNNVTC 1848 1843 1816 1811 1836 1839 + MnlI CCTC 1846 1845 . . 1832 1836 - FokI GGATG 1818 1822 . . 1832 1836 - BseGI GGATG 1831 1829 . . 1855 1858 - AciI CCGC 1855 1857 . . 1865 1870 + SecI* CCNNGG 1865 1869 . . 1866 1870 + BssKI CCNGG 1865 1870 . . 1866 1870 + BseBI CCWGG 1867 1868 . . 1866 1870 + ScrFI CCNGG 1867 1868 . . 1866 1870 + EcoRII CCWGG 1865 1870 . . 1868 1871 + SetI ASST 1871 1867 . . 1893 1896 + CviRI* TGCA 1894 1894 . . 1898 1901 + CviRI* TGCA 1899 1899 . . 1895 1900 - BsrDI GCAATG 1894 1892 . . 1907 1910 + MnlI CCTC 1917 1916 . . 1915 1919 + SfaNI GCATC 1924 1928 . . 1921 1925 - MboII GAAGA 1913 1912 . . 1920 1925 - BbvII* GAAGAC 1913 1917 . . 1933 1937 - Hin4II* CCTTC 1927 1926 . . 1949 1952 + MaeI CTAG 1949 1951 . . 1956 1959 + SetI ASST 1959 1955 . . 1969 1972 + SetI ASST 1972 1968 . . 1969 1972 + CviJI RGCY 1970 1970 . . 1969 1972 + AluI AGCT 1970 1970 . . 1990 1994 + Hin4II* CCTTC 2000 1999 . . 1996 1999 + TaqI TCGA 1996 1998 . . 1993 1997 - MboII GAAGA 1985 1984 . . 2008 2013 + EcoRI GAATTC 2008 2012 . . 2008 2013 + ApoI RAATTY 2008 2012 . . 2009 2012 + TspEI AATT 2008 2012 . . 2018 2021 + MnlI CCTC 2028 2027 . . 2014 2018 - HphI GGTGA 2006 2005 . . 2021 2025 + DdeI CTNAG 2021 2024 . . 2021 2025 + BspCNI CTCAG 2034 2032 . . 2021 2025 + BseMII CTCAG 2035 2033 . . 2022 2026 + Hpy188I TCNGA 2024 2023 . . 2025 2035 + Hin4I GAYNNNNNVTC 2016 2011 2048 2043 2038 2042 + TspDTI ATGAA 2053 2051 . . 2040 2045 + EcoRI GAATTC 2040 2044 . . 2040 2045 + ApoI RAATTY 2040 2044 . . 2041 2044 + TspEI AATT 2040 2044 . . 2046 2049 + CviRI* TGCA 2047 2047 . . 2047 2052 + Cac8I GCNNGC 2049 2049 . . 2050 2053 + SetI ASST 2053 2049 . . 2050 2053 + CviJI RGCY 2051 2051 . . 2050 2053 + AluI AGCT 2051 2051 . . 2060 2064 + DdeI CTNAG 2060 2063 . . 2060 2064 + BspCNI CTCAG 2073 2071 . . 2060 2064 + BseMII CTCAG 2074 2072 . . 2063 2068 + TatI WGTACW 2063 2067 . . 2064 2069 + Hpy166II GTNNAC 2066 2066 . . 2064 2067 + Csp6I GTAC 2064 2066 . . 2064 2067 + RsaI GTAC 2065 2065 . . 2079 2085 + TspRI CASTGNN 2085 2076 . . 2075 2079 - FokI GGATG 2061 2065 . . 2075 2079 - BseGI GGATG 2074 2072 . . 2080 2084 + BsrI ACTGG 2085 2083 . . 2084 2089 + Hpy166II GTNNAC 2086 2086 . . 2084 2089 + BssNAI GTATAC 2086 2086 . . 2084 2089 + AccI GTMKAC 2085 2087 . . 2093 2097 + DdeI CTNAG 2093 2096 . . 2093 2097 + BspCNI CTCAG 2106 2104 . . 2093 2097 + BseMII CTCAG 2107 2105 . . 2096 2099 + SetI ASST 2099 2095 . . 2101 2105 + Hpy188I TCNGA 2103 2102 . . 2106 2115 + MslI CAYNNNNRTG 2110 2110 . . 2106 2115 + OliI CACNNNNGTG 2110 2110 . . 2144 2149 + SmlI CTYRAG 2144 2148 . . 2144 2149 - Bce83I* CTTGAG 2129 2127 . . 2152 2156 + Hpy188I TCNGA 2154 2153 . . 2161 2165 - BsrI ACTGG 2161 2159 . . 2195 2200 + SmlI CTYRAG 2195 2199 . . 2195 2200 - Bce83I* CTTGAG 2180 2178 . . 2203 2207 + Hpy188I TCNGA 2205 2204 . . 2211 2216 + Cfr10I RCCGGY 2211 2215 . . 2211 2216 + BetI* WCCGGW 2211 2215 . . 2211 2216 + AgeI ACCGGT 2211 2215 . . 2212 2215 + HpaII CCGG 2212 2214 . . 2246 2251 + SmlI CTYRAG 2246 2250 . . 2246 2251 - Bce83I* CTTGAG 2231 2229 . . 2254 2258 + Hpy188I TCNGA 2256 2255 . . 2263 2267 - BsrI ACTGG 2263 2261 . . 2286 2290 - TspDTI ATGAA 2276 2274 . . 2297 2302 + SmlI CTYRAG 2297 2301 . . 2297 2302 - Bce83I* CTTGAG 2282 2280 . . 2305 2309 + Hpy188I TCNGA 2307 2306 . . 2313 2318 + Cfr10I RCCGGY 2313 2317 . . 2313 2318 + BetI* WCCGGW 2313 2317 . . 2313 2318 + AgeI ACCGGT 2313 2317 . . 2314 2317 + HpaII CCGG 2314 2316 . . 2341 2345 - TspGWI ACGGA 2331 2329 . . 2348 2353 + SmlI CTYRAG 2348 2352 . . 2348 2353 - Bce83I* CTTGAG 2333 2331 . . 2356 2360 + Hpy188I TCNGA 2358 2357 . . 2360 2365 + AflIII ACRYGT 2360 2364 . . 2361 2366 + BtrI CACGTC 2363 2363 . . 2362 2365 + TaiI ACGT 2365 2361 . . 2362 2365 + SetI ASST 2365 2361 . . 2362 2365 + MaeII ACGT 2362 2364 . . 2362 2372 + BaeI ACNNNNGTAYC 2351 2346 2384 2379 2368 2371 + Csp6I GTAC 2368 2370 . . 2368 2371 + RsaI GTAC 2369 2369 . . 2370 2373 + SetI ASST 2373 2369 . . 2371 2375 + Hin4II* CCTTC 2381 2380 . . 2383 2388 + Hpy178III* TCNNGA 2384 2386 . . 2387 2390 + DpnI GATC 2388 2388 . . 2387 2390 + BstKTI GATC 2389 2387 . . 2387 2390 + MboI GATC 2386 2390 . . 2392 2396 - TspGWI ACGGA 2382 2380 . . 2399 2404 + SmlI CTYRAG 2399 2403 . . 2399 2404 - Bce83I* CTTGAG 2384 2382 . . 2404 2408 + BsmAI GTCTC 2409 2413 . . 2406 2410 + DdeI CTNAG 2406 2409 . . 2406 2410 + BspCNI CTCAG 2419 2417 . . 2406 2410 + BseMII CTCAG 2420 2418 . . 2407 2411 + Hpy188I TCNGA 2409 2408 . . 2412 2421 + MslI CAYNNNNRTG 2416 2416 . . 2412 2421 + OliI CACNNNNGTG 2416 2416 . . 2415 2420 + BetI* WCCGGW 2415 2419 . . 2416 2419 + HpaII CCGG 2416 2418 . . 2418 2423 + NlaIV GGNNCC 2420 2420 . . 2418 2423 + HgiCI* GGYRCC 2418 2422 . . 2422 2426 + Hin4II* CCTTC 2432 2431 . . 2437 2440 + SetI ASST 2440 2436 . . 2443 2447 - TspGWI ACGGA 2433 2431 . . 2459 2462 + MaeI CTAG 2459 2461 . . 2461 2464 + SetI ASST 2464 2460 . . 2461 2464 + CviJI RGCY 2462 2462 . . 2461 2464 + AluI AGCT 2462 2462 . . 2467 2472 + SfeI* CTRYAG 2467 2471 . . 2471 2474 + CviJI RGCY 2472 2472 . . 2486 2489 + MnlI CCTC 2496 2495 . . 2483 2487 - FokI GGATG 2469 2473 . . 2483 2487 - BseGI GGATG 2482 2480 . . 2492 2497 + AclI AACGTT 2493 2495 . . 2493 2496 + TaiI ACGT 2496 2492 . . 2493 2496 + SetI ASST 2496 2492 . . 2493 2496 + MaeII ACGT 2493 2495 . . 2498 2502 - HphI GGTGA 2490 2489 . . 2512 2515 + FatI CATG 2511 2515 . . 2512 2515 + NlaIII CATG 2515 2511 . . 2512 2515 + CviAII CATG 2512 2514 . . 2517 2522 + HaeII RGCGCY 2521 2517 . . 2517 2522 + Eco47III AGCGCT 2519 2519 . . 2518 2521 + Hin6I GCGC 2518 2520 . . 2518 2521 + GlaI GCGC 2519 2519 . . 2518 2521 + HhaI GCGC 2520 2518 . . 2524 2528 + Hpy188I TCNGA 2526 2525 . . 2541 2546 + TsoI TARCCA 2557 2555 . . 2542 2545 + CviJI RGCY 2543 2543 . . 2549 2554 + HindIII AAGCTT 2549 2553 . . 2550 2553 + SetI ASST 2553 2549 . . 2550 2553 + CviJI RGCY 2551 2551 . . 2550 2553 + AluI AGCT 2551 2551 . . 2554 2558 + Hpy188I TCNGA 2556 2555 . . 2555 2559 + DdeI CTNAG 2555 2558 . . 2555 2559 - BspCNI CTCAG 2547 2545 . . 2555 2559 - BseMII CTCAG 2546 2544 . . 2564 2567 + MaeI CTAG 2564 2566 . . 2566 2569 + SetI ASST 2569 2565 . . 2566 2569 + CviJI RGCY 2567 2567 . . 2566 2569 + AluI AGCT 2567 2567 . . 2577 2580 + CviRI* TGCA 2578 2578 . . 2583 2586 + SetI ASST 2586 2582 . . 2583 2586 + CviJI RGCY 2584 2584 . . 2583 2586 + AluI AGCT 2584 2584 . . 2587 2591 + Hpy188I TCNGA 2589 2588 . . 2600 2604 + SfaNI GCATC 2609 2613 . . 2603 2608 + Hpy178III* TCNNGA 2604 2606 . . 2601 2605 - FokI GGATG 2587 2591 . . 2601 2605 - BseGI GGATG 2600 2598 . . 2612 2617 + NspBII* CMGCKG 2614 2614 . . 2612 2617 + SacII CCGCGG 2615 2613 . . 2612 2617 + DsaI* CCRYGG 2612 2616 . . 2612 2617 + SecI* CCNNGG 2612 2616 . . 2612 2615 + AciI CCGC 2612 2614 . . 2613 2616 + FnuDII* CGCG 2614 2614 . . 2614 2618 + TauI GCSGC 2617 2614 . . 2614 2618 + BlsI GCNGC 2616 2615 . . 2614 2618 + BisI GCNGC 2615 2616 . . 2616 2619 + CviJI RGCY 2617 2617 . . 2614 2617 - AciI CCGC 2614 2616 . . 2646 2650 + Hpy99I CGWCG 2650 2645 . . 2647 2651 + HgaI GACGC 2656 2661 . . 2655 2660 + EcoP15I CAGCAG 2685 2687 . . 2669 2672 + AciI CCGC 2669 2671 . . 2669 2674 + BsrBI CCGCTC 2671 2671 . . 2689 2692 + TaiI ACGT 2692 2688 . . 2689 2692 + SetI ASST 2692 2688 . . 2689 2692 + MaeII ACGT 2689 2691 . . 2691 2696 + Hpy166II GTNNAC 2693 2693 . . 2691 2696 + AccI GTMKAC 2692 2694 . . 2704 2708 + BccI CCATC 2712 2713 . . 2707 2711 - MboII GAAGA 2699 2698 . . 2698 2711 - AjuI GAANNNNNNNTTGG 2723 2718 2691 2686 2716 2720 + SfaNI GCATC 2725 2729 . . 2720 2723 + MnlI CCTC 2730 2729 . . 2717 2721 - FokI GGATG 2703 2707 . . 2717 2721 - BseGI GGATG 2716 2714 . . 2737 2741 + BccI CCATC 2745 2746 . . 2747 2750 + MnlI CCTC 2757 2756 . . 2744 2748 - FokI GGATG 2730 2734 . . 2744 2748 - BseGI GGATG 2743 2741 . . 2752 2755 + MseI TTAA 2752 2754 . . 2759 2762 + SetI ASST 2762 2758 . . 2778 2783 + TatI WGTACW 2778 2782 . . 2778 2783 + Bsp1407I TGTACA 2778 2782 . . 2779 2782 + Csp6I GTAC 2779 2781 . . 2779 2782 + RsaI GTAC 2780 2780 . . 2791 2794 + MseI TTAA 2791 2793 . . 2797 2801 + TspGWI ACGGA 2812 2810 . . 2800 2804 + TfiI GAWTC 2800 2803 . . 2800 2804 + HinfI GANTC 2800 2803 . . 2803 2806 + TaqI TCGA 2803 2805 . . 2804 2808 + Hpy99I CGWCG 2808 2803 . . 2807 2812 + McrI* CGRYCG 2810 2808 . . 2809 2813 + Tsp4CI* ACNGT 2811 2810 . . 2821 2825 + BccI CCATC 2829 2830 . . 2834 2838 + Tsp4CI* ACNGT 2836 2835 . . 2843 2846 + Csp6I GTAC 2843 2845 . . 2843 2846 + RsaI GTAC 2844 2844 . . 2840 2844 - BsrI ACTGG 2840 2838 . . 2845 2848 + TaiI ACGT 2848 2844 . . 2845 2848 + SetI ASST 2848 2844 . . 2845 2848 + MaeII ACGT 2845 2847 . . 2857 2861 + Tsp45I GTSAC 2856 2861 . . 2857 2861 + MaeIII GTNAC 2856 2861 . . 2867 2871 + Tsp45I GTSAC 2866 2871 . . 2867 2871 + MaeIII GTNAC 2866 2871 . . 2874 2879 + MfeI CAATTG 2874 2878 . . 2875 2878 + TspEI AATT 2874 2878 . . 2883 2887 + TfiI GAWTC 2883 2886 . . 2883 2887 + HinfI GANTC 2883 2886 . . 2886 2891 + MmeI TCCRAC 2911 2909 . . 2891 2895 + DdeI CTNAG 2891 2894 . . 2891 2895 - BspCNI CTCAG 2883 2881 . . 2891 2895 - BseMII CTCAG 2882 2880 . . 2896 2899 + MseI TTAA 2896 2898 . . 2904 2909 + Cac8I GCNNGC 2906 2906 . . 2907 2912 + NlaIV GGNNCC 2909 2909 . . 2907 2912 + HgiCI* GGYRCC 2907 2911 . . 2908 2918 + MwoI GCNNNNNNNGC 2914 2911 . . 2912 2920 + AlwNI CAGNNNCTG 2917 2914 . . 2916 2919 + SetI ASST 2919 2915 . . 2916 2919 + CviJI RGCY 2917 2917 . . 2916 2919 + AluI AGCT 2917 2917 . . 2917 2921 + BlsI GCNGC 2919 2918 . . 2917 2921 + BisI GCNGC 2918 2919 . . 2917 2921 + TseI GCWGC 2917 2920 . . 2919 2922 + CviRI* TGCA 2920 2920 . . 2920 2924 + SfaNI GCATC 2929 2933 . . 2920 2930 + MwoI GCNNNNNNNGC 2926 2923 . . 2920 2930 + BstAPI GCANNNNNTGC 2926 2923 . . 2917 2921 - BbvI GCAGC 2904 2908 . . 2925 2931 + TspRI CASTGNN 2931 2922 . . 2921 2925 - FokI GGATG 2907 2911 . . 2921 2925 - BseGI GGATG 2920 2918 . . 2928 2931 + CviRI* TGCA 2929 2929 . . 2929 2933 + SfaNI GCATC 2938 2942 . . 2925 2930 - BtsI GCAGTG 2924 2922 . . 2932 2936 - MboII GAAGA 2924 2923 . . 2938 2942 + Tsp4CI* ACNGT 2940 2939 . . 2941 2946 - MmeI TCCRAC 2922 2920 . . 2945 2948 - MnlI CCTC 2938 2937 . . 2971 2974 + FatI CATG 2970 2974 . . 2971 2974 + NlaIII CATG 2974 2970 . . 2971 2974 + CviAII CATG 2971 2973 . . 2973 2976 + CviRI* TGCA 2974 2974 . . 2974 2984 + MwoI GCNNNNNNNGC 2980 2977 . . 2975 2980 + MfeI CAATTG 2975 2979 . . 2976 2979 + TspEI AATT 2975 2979 . . 2979 2982 + CviRI* TGCA 2980 2980 . . 2980 2984 + BlsI GCNGC 2982 2981 . . 2980 2984 + BisI GCNGC 2981 2982 . . 2980 2984 + BbvI GCAGC 2992 2996 . . 2980 2984 + TseI GCWGC 2980 2983 . . 2982 2985 + CviJI RGCY 2983 2983 . . 2983 2987 + TauI GCSGC 2986 2983 . . 2983 2987 + BlsI GCNGC 2985 2984 . . 2983 2987 + BisI GCNGC 2984 2985 . . 2984 2987 + AciI CCGC 2984 2986 . . 2985 2988 + FnuDII* CGCG 2986 2986 . . 2997 3002 + Hpy178III* TCNNGA 2998 3000 . . 2998 3003 + BmeT110I CYCGRG 2999 3001 . . 2998 3003 + AvaI CYCGRG 2998 3002 . . 3002 3005 + CviJI RGCY 3003 3003 . . 3004 3010 + SauI* CCTNAGG 3005 3008 . . 3005 3009 + DdeI CTNAG 3005 3008 . . 3005 3009 - BspCNI CTCAG 2997 2995 . . 3005 3009 - BseMII CTCAG 2996 2994 . . 3007 3010 - MnlI CCTC 3000 2999 . . 3010 3013 + CviJI RGCY 3011 3011 . . 3041 3046 + HindIII AAGCTT 3041 3045 . . 3042 3045 + SetI ASST 3045 3041 . . 3042 3045 + CviJI RGCY 3043 3043 . . 3042 3045 + AluI AGCT 3043 3043 . . 3055 3060 + ApoI RAATTY 3055 3059 . . 3056 3059 + TspEI AATT 3055 3059 . . 3067 3071 + Hpy188I TCNGA 3069 3068 . . 3084 3087 + CviJI RGCY 3085 3085 . . 3095 3099 + Hpy188I TCNGA 3097 3096 . . 3092 3096 - MboII GAAGA 3084 3083 . . 3097 3102 - BciVI GTATCC 3091 3090 . . 3107 3112 + Eco57MI CTGRAG 3128 3126 . . 3107 3112 + Eco57I CTGAAG 3128 3126 . . 3111 3114 + SetI ASST 3114 3110 . . 3111 3114 + CviJI RGCY 3112 3112 . . 3111 3114 + AluI AGCT 3112 3112 . . 3114 3118 + Hpy188I TCNGA 3116 3115 . . 3123 3126 + SetI ASST 3126 3122 . . 3128 3131 + MseI TTAA 3128 3130 . . 3132 3141 + XmnI GAANNNNTTC 3136 3136 . . 3134 3137 + TaiI ACGT 3137 3133 . . 3134 3137 + SetI ASST 3137 3133 . . 3134 3137 + MaeII ACGT 3134 3136 . . 3151 3155 + Tsp4CI* ACNGT 3153 3152 . . 3154 3157 + Csp6I GTAC 3154 3156 . . 3154 3157 + RsaI GTAC 3155 3155 . . 3177 3181 + FokI GGATG 3190 3194 . . 3177 3181 + BseGI GGATG 3183 3181 . . 3181 3185 + HinfI GANTC 3181 3184 . . 3181 3185 - PleI GAGTC 3175 3176 . . 3181 3185 - MlyI GAGTC 3175 3175 . . 3195 3199 + DdeI CTNAG 3195 3198 . . 3209 3214 + Eco57MI CTGRAG 3230 3228 . . 3209 3214 + Eco57I CTGAAG 3230 3228 . . 3214 3219 + Hpy166II GTNNAC 3216 3216 . . 3214 3219 + BssNAI GTATAC 3216 3216 . . 3214 3219 + AccI GTMKAC 3215 3217 . . 3222 3227 + MmeI TCCRAC 3247 3245 . . 3227 3232 + PstI CTGCAG 3231 3227 . . 3227 3232 + SfeI* CTRYAG 3227 3231 . . 3228 3231 + CviRI* TGCA 3229 3229 . . 3238 3243 + BseRI GAGGAG 3253 3251 . . 3238 3241 - MnlI CCTC 3231 3230 . . 3242 3245 + SetI ASST 3245 3241 . . 3242 3245 + CviJI RGCY 3243 3243 . . 3242 3245 + AluI AGCT 3243 3243 . . 3238 3248 - Hin4I GAYNNNNNVTC 3261 3256 3229 3224 3255 3258 + TspEI AATT 3254 3258 . . 3264 3269 + Ksp632I* CTCTTC 3270 3273 . . 3265 3269 - MboII GAAGA 3257 3256 . . 3271 3276 + HaeII RGCGCY 3275 3271 . . 3271 3276 + Eco47III AGCGCT 3273 3273 . . 3272 3275 + Hin6I GCGC 3272 3274 . . 3272 3275 + GlaI GCGC 3273 3273 . . 3272 3275 + HhaI GCGC 3274 3272 . . 3283 3287 + TspGWI ACGGA 3298 3296 . . 3285 3289 + FokI GGATG 3298 3302 . . 3285 3289 + BseGI GGATG 3291 3289 . . 3286 3290 - BccI CCATC 3280 3281 . . 3296 3299 + Csp6I GTAC 3296 3298 . . 3296 3299 + RsaI GTAC 3297 3297 . . 3312 3315 + CviJI RGCY 3313 3313 . . 3313 3317 + BlsI GCNGC 3315 3314 . . 3313 3317 + BisI GCNGC 3314 3315 . . 3313 3317 + TseI GCWGC 3313 3316 . . 3315 3318 + CviRI* TGCA 3316 3316 . . 3316 3321 + BsrDI GCAATG 3323 3321 . . 3313 3317 - BbvI GCAGC 3300 3304 . . 3325 3329 + TfiI GAWTC 3325 3328 . . 3325 3329 + HinfI GANTC 3325 3328 . . 3329 3332 + MnlI CCTC 3339 3338 . . 3337 3347 + BsiYI* CCNNNNNNNGG 3343 3340 . . 3337 3340 + AciI CCGC 3337 3339 . . 3345 3349 + BmgT120I GGNCC 3346 3347 . . 3345 3349 + AsuI* GGNCC 3345 3348 . . 3346 3349 + CviJI RGCY 3347 3347 . . 3346 3349 + HaeIII GGCC 3347 3347 . . 3348 3351 + MnlI CCTC 3358 3357 . . 3362 3366 + MaeIII GTNAC 3361 3366 . . 3368 3378 + MwoI GCNNNNNNNGC 3374 3371 . . 3376 3381 + EciI GGCGGA 3392 3390 . . 3379 3384 + XhoII RGATCY 3379 3383 . . 3379 3383 + BinI* GGATC 3387 3388 . . 3380 3383 + DpnI GATC 3381 3381 . . 3380 3383 + BstKTI GATC 3382 3380 . . 3380 3383 + MboI GATC 3379 3383 . . 3377 3380 - AciI CCGC 3377 3379 . . 3385 3389 + Hpy188I TCNGA 3387 3386 . . 3382 3386 - MboII GAAGA 3374 3373 . . 3388 3398 + Hin4I GAYNNNNNVTC 3379 3374 3411 3406 3389 3393 + TspGWI ACGGA 3404 3402 . . 3389 3399 + BsaXI ACNNNNNCTCC 3379 3376 3409 3406 3391 3396 + XhoII RGATCY 3391 3395 . . 3391 3396 + NlaIV GGNNCC 3393 3393 . . 3391 3396 + BamHI GGATCC 3391 3395 . . 3391 3395 + BinI* GGATC 3399 3400 . . 3392 3395 + DpnI GATC 3393 3393 . . 3392 3395 + BstKTI GATC 3394 3392 . . 3392 3395 + MboI GATC 3391 3395 . . 3395 3398 + MnlI CCTC 3405 3404 . . 3392 3396 - BinI* GGATC 3386 3387 . . 3398 3401 + MnlI CCTC 3408 3407 . . 3396 3401 - BseRI GAGGAG 3387 3385 . . 3399 3404 - Eco57MI CTGRAG 3384 3382 . . 3399 3404 - GsuI CTGGAG 3384 3382 . . 3401 3405 - BsrI ACTGG 3401 3399 . . 3406 3409 + TspEI AATT 3405 3409 . . 3408 3413 + AsuII TTCGAA 3409 3411 . . 3409 3412 + TaqI TCGA 3409 3411 . . 3414 3418 + DdeI CTNAG 3414 3417 . . 3414 3418 + BspCNI CTCAG 3427 3425 . . 3414 3418 + BseMII CTCAG 3428 3426 . . 3415 3420 + Hpy178III* TCNNGA 3416 3418 . . 3418 3423 + XhoII RGATCY 3418 3422 . . 3418 3422 + BinI* GGATC 3426 3427 . . 3419 3422 + DpnI GATC 3420 3420 . . 3419 3422 + BstKTI GATC 3421 3419 . . 3419 3422 + MboI GATC 3418 3422 . . 3421 3425 - MboII GAAGA 3413 3412 . . 3426 3429 + SetI ASST 3429 3425 . . 3422 3427 - Eco57MI CTGRAG 3407 3405 . . 3422 3427 - Eco57I CTGAAG 3407 3405 . . 3430 3435 + Hpy178III* TCNNGA 3431 3433 . . 3433 3438 + XhoII RGATCY 3433 3437 . . 3433 3437 + BinI* GGATC 3441 3442 . . 3434 3437 + DpnI GATC 3435 3435 . . 3434 3437 + BstKTI GATC 3436 3434 . . 3434 3437 + MboI GATC 3433 3437 . . 3439 3442 + TspEI AATT 3438 3442 . . 3441 3446 + AsuII TTCGAA 3442 3444 . . 3442 3445 + TaqI TCGA 3442 3444 . . 3448 3453 + BetI* WCCGGW 3448 3452 . . 3449 3452 + HpaII CCGG 3449 3451 . . 3457 3461 - MboII GAAGA 3449 3448 . . 3456 3461 - BbvII* GAAGAC 3449 3453 . . 3459 3463 - TspDTI ATGAA 3449 3447 . . 3466 3471 + Hpy178III* TCNNGA 3467 3469 . . 3466 3471 + BetI* WCCGGW 3466 3470 . . 3466 3471 + BspMII* TCCGGA 3466 3470 . . 3463 3467 - MboII GAAGA 3455 3454 . . 3467 3470 + HpaII CCGG 3467 3469 . . 3471 3476 + ApoI RAATTY 3471 3475 . . 3472 3475 + TspEI AATT 3471 3475 . . 3480 3485 + Hpy178III* TCNNGA 3481 3483 . . 3487 3492 + Cfr10I RCCGGY 3487 3491 . . 3484 3488 - SfaNI GCATC 3474 3478 . . 3488 3491 + HpaII CCGG 3488 3490 . . 3491 3494 + Csp6I GTAC 3491 3493 . . 3491 3494 + RsaI GTAC 3492 3492 . . 3500 3505 + SspI AATATT 3502 3502 . . 3504 3508 - TspDTI ATGAA 3494 3492 . . 3506 3510 - FokI GGATG 3492 3496 . . 3506 3510 - BseGI GGATG 3505 3503 . . 3520 3525 + MmeI TCCRAC 3545 3543 . . 3524 3527 + TaiI ACGT 3527 3523 . . 3524 3527 + SetI ASST 3527 3523 . . 3524 3527 + MaeII ACGT 3524 3526 . . 3526 3531 + BciVI GTATCC 3537 3536 . . 3536 3539 + MaeI CTAG 3536 3538 . . 3589 3593 + FokI GGATG 3602 3606 . . 3589 3593 + BseGI GGATG 3595 3593 . . 3590 3594 - SfaNI GCATC 3580 3584 . . 3608 3613 + Hpy178III* TCNNGA 3609 3611 . . 3608 3613 + BspHI TCATGA 3608 3612 . . 3609 3612 + FatI CATG 3608 3612 . . 3609 3612 + NlaIII CATG 3612 3608 . . 3609 3612 + CviAII CATG 3609 3611 . . 3607 3611 - TspDTI ATGAA 3597 3595 . . 3641 3646 + EciI GGCGGA 3657 3655 . . 3638 3642 - Hin4II* CCTTC 3632 3631 . . 3645 3649 + TfiI GAWTC 3645 3648 . . 3645 3649 + HinfI GANTC 3645 3648 . . 3645 3655 + Hin4I GAYNNNNNVTC 3636 3631 3668 3663 3642 3645 - AciI CCGC 3642 3644 . . 3654 3659 + Hpy178III* TCNNGA 3655 3657 . . 3645 3655 - Hin4I GAYNNNNNVTC 3668 3663 3636 3631 3660 3663 + TspEI AATT 3659 3663 . . 3665 3670 + Hpy178III* TCNNGA 3666 3668 . . 3677 3682 + ApoI RAATTY 3677 3681 . . 3678 3681 + TspEI AATT 3677 3681 . . 3683 3687 - BsrI ACTGG 3683 3681 . . 3700 3705 + ApoI RAATTY 3700 3704 . . 3701 3704 + TspEI AATT 3700 3704 . . 3718 3721 + TspEI AATT 3717 3721 . . 3728 3732 + TspDTI ATGAA 3743 3741 . . 3747 3752 + Hpy178III* TCNNGA 3748 3750 . . 3751 3755 + TfiI GAWTC 3751 3754 . . 3751 3755 + HinfI GANTC 3751 3754 . . 3773 3778 + EcoRI GAATTC 3773 3777 . . 3773 3786 + AjuI GAANNNNNNNTTGG 3765 3760 3797 3792 3773 3778 + ApoI RAATTY 3773 3777 . . 3774 3777 + TspEI AATT 3773 3777 . . 3781 3784 + TspEI AATT 3780 3784 . . 3785 3789 + FokI GGATG 3798 3802 . . 3785 3789 + BseGI GGATG 3791 3789 . . 3789 3793 + FokI GGATG 3802 3806 . . 3789 3793 + BseGI GGATG 3795 3793 . . 3786 3790 - BccI CCATC 3780 3781 . . 3797 3801 + MaeIII GTNAC 3796 3801 . . 3806 3810 + Tsp45I GTSAC 3805 3810 . . 3806 3810 + MaeIII GTNAC 3805 3810 . . 3813 3818 + Hpy166II GTNNAC 3815 3815 . . 3813 3818 + HindII GTYRAC 3815 3815 . . 3826 3830 + HinfI GANTC 3826 3829 . . 3826 3830 - PleI GAGTC 3820 3821 . . 3826 3830 - MlyI GAGTC 3820 3820 . . 3834 3839 + MmeI TCCRAC 3859 3857 . . 3839 3843 + BssKI CCNGG 3838 3843 . . 3839 3843 + BseBI CCWGG 3840 3841 . . 3839 3843 + ScrFI CCNGG 3840 3841 . . 3839 3843 + EcoRII CCWGG 3838 3843 . . 3853 3856 + TaqI TCGA 3853 3855 . . 3869 3874 + ApoI RAATTY 3869 3873 . . 3870 3873 + TspEI AATT 3869 3873 . . 3874 3879 + Hpy178III* TCNNGA 3875 3877 . . 3881 3886 + MfeI CAATTG 3881 3885 . . 3882 3885 + TspEI AATT 3881 3885 . . 3886 3891 + Cac8I GCNNGC 3888 3888 . . 3886 3891 + BmtI GCTAGC 3890 3886 . . 3886 3891 + NheI GCTAGC 3886 3890 . . 3887 3890 + MaeI CTAG 3887 3889 . . 3888 3893 + TsoI TARCCA 3904 3902 . . 3889 3892 + CviJI RGCY 3890 3890 . . 3899 3905 + PasI CCCWGGG 3900 3903 . . 3899 3904 + SecI* CCNNGG 3899 3903 . . 3899 3909 + BsiYI* CCNNNNNNNGG 3905 3902 . . 3900 3904 + BssKI CCNGG 3899 3904 . . 3900 3904 + BseBI CCWGG 3901 3902 . . 3900 3905 + SecI* CCNNGG 3900 3904 . . 3900 3904 + ScrFI CCNGG 3901 3902 . . 3900 3904 + EcoRII CCWGG 3899 3904 . . 3905 3910 - MmeI TCCRAC 3886 3884 . . #--------------------------------------- # # # AarI: # [CACCTGC] # 249 1549 2123 # # AccI: # [GTMKAC] # 352 363 410 523 607 706 # 960 # # AciI: # [CCGC] # 2 40 43 55 161 166 # 185 221 232 263 265 279 # 315 353 584 757 # # AclI: # [AACGTT] # 1428 2493 # # AcyI: # [GRCGYC] # 1379 2542 # # AflIII: # [ACRYGT] # 1561 2360 # # AgeI: # [ACCGGT] # 102 1608 2211 # # AjuI: # [GAANNNNNNNTTGG] # 32 32 124 1042 2691 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 199 3688 # # AluI: # [AGCT] # 4 4 9 11 15 16 # 17 48 51 59 69 78 # 81 89 96 103 111 124 # 126 131 144 198 201 202 # 207 305 333 411 678 # # AlwNI: # [CAGNNNCTG] # 448 1004 1203 1266 # # ApaLI: # [GTGCAC] # 536 3385 # # ApoI: # [RAATTY] # 23 32 52 73 96 206 # 316 416 725 967 1015 # # AsuI*: # [GGNCC] # 5 54 576 3286 # # AsuII: # [TTCGAA] # 33 479 3409 # # AvaI: # [CYCGRG] # 56 923 1213 1729 # # BaeI: # [ACNNNNGTAYC] # 33 1537 2351 # # BamHI: # [GGATCC] # 530 3391 # # BbvI: # [GCAGC] # 43 73 88 91 144 185 # 308 411 621 676 1281 # # BbvII*: # [GAAGAC] # 472 1536 1913 # # BccI: # [CCATC] # 18 33 84 104 114 117 # 141 234 261 378 451 493 # 500 993 # # Bce83I*: # [CTTGAG] # 51 51 51 51 51 51 # 179 399 405 410 685 1537 # # BceAI: # [ACGGC] # 310 3611 # # BcgI: # [CGANNNNNNTGC] # 34 34 34 406 570 599 # 2244 # # BciVI: # [GTATCC] # 384 446 3091 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 136 3751 # # BetI*: # [WCCGGW] # 18 102 102 455 687 1033 # 1524 # # BinI*: # [GGATC] # 1 12 15 27 480 3386 # # BisI: # [GCNGC] # 3 18 25 48 63 66 # 144 210 303 330 425 607 # 701 978 # # BlsI: # [GCNGC] # 3 18 25 48 63 66 # 144 210 303 330 426 606 # 701 978 # # BmeT110I: # [CYCGRG] # 57 922 1213 1729 # # BmgT120I: # [GGNCC] # 5 55 575 3286 # # BmtI: # [GCTAGC] # 4 31 162 198 216 263 # 929 2118 # # BplI: # [GAGNNNNNCTC] # 0 0 32 525 3364 # # BsaXI: # [ACNNNNNCTCC] # 30 30 512 1497 1852 # # BseBI: # [CCWGG] # 20 61 1867 1973 # # BseGI: # [GGATG] # 4 27 90 108 116 118 # 120 126 177 180 196 214 # 243 263 378 408 1153 # # BseMII: # [CTCAG] # 33 39 51 75 75 111 # 114 118 126 228 313 336 # 419 432 493 958 # # BseRI: # [GAGGAG] # 134 324 534 1335 1594 # # BseSI: # [GKGCMC] # 540 3381 # # BsiYI*: # [CCNNNNNNNGG] # 16 562 3343 # # BsmAI: # [GTCTC] # 25 58 356 434 667 869 # 1512 # # Bsp1407I: # [TGTACA] # 1143 2778 # # BspCNI: # [CTCAG] # 33 39 51 75 75 113 # 114 117 128 228 313 336 # 417 430 494 958 # # BspHI: # [TCATGA] # 313 3608 # # BspMI: # [ACCTGC] # 249 1549 2123 # # BspMII*: # [TCCGGA] # 455 3466 # # BsrBI: # [CCGCTC] # 1250 2671 # # BsrDI: # [GCAATG] # 93 598 1429 1801 # # BsrI: # [ACTGG] # 69 76 90 92 102 112 # 123 123 132 213 238 277 # 282 389 465 561 577 # # BssKI: # [CCNGG] # 1 22 55 61 1809 1973 # # BssNAI: # [GTATAC] # 705 1130 2086 # # BstAPI: # [GCANNNNNTGC] # 995 1113 1813 # # BstKTI: # [GATC] # 12 15 27 485 993 2389 # # BstXI: # [CCANNNNNNTGG] # 83 177 3661 # # BtrI: # [CACGTC] # 1558 2363 # # BtsI: # [GCAGTG] # 31 253 344 458 716 997 # 1122 # # Cac8I: # [GCNNGC] # 4 33 53 54 63 108 # 198 210 210 216 279 654 # 857 982 # # CauII*: # [CCSGG] # 1 57 3863 # # Cfr10I: # [RCCGGY] # 102 434 1174 2211 # # Cfr9I: # [CCCGGG] # 56 3865 # # CfrI: # [YGGCCR] # 413 3508 # # Csp6I: # [GTAC] # 30 42 51 64 81 142 # 158 168 183 195 204 304 # 311 319 377 411 430 451 # # CviAII: # [CATG] # 312 459 638 669 903 940 # # CviJI: # [RGCY] # 4 4 4 6 7 8 # 8 9 10 11 15 16 # 17 19 20 27 29 30 # 31 31 32 33 34 36 # 37 38 38 40 41 42 # 43 46 47 48 51 55 # 60 66 70 71 71 81 # 83 88 104 108 111 131 # 142 144 169 201 300 411 # 543 # # CviRI*: # [TGCA] # 3 5 6 9 9 15 # 31 44 45 78 85 87 # 96 109 114 148 155 217 # 222 249 300 342 416 531 # 605 # # DdeI: # [CTNAG] # 33 39 51 75 75 104 # 114 134 149 190 219 228 # 313 336 396 507 958 # # DpnI: # [GATC] # 12 15 27 486 993 2388 # # DrdI: # [GACNNNNNNGTC] # 294 3627 # # DsaI*: # [CCRYGG] # 1309 2612 # # EciI: # [GGCGGA] # 264 265 760 2632 # # Ecl136II: # [GAGCTC] # 275 3646 # # Eco47III: # [AGCGCT] # 648 754 2519 # # Eco57I: # [CTGAAG] # 102 177 387 514 1352 1389 # # Eco57MI: # [CTGRAG] # 23 102 154 255 371 387 # 514 726 1389 # # EcoP15I: # [CAGCAG] # 85 141 595 646 1218 1236 # # EcoRI: # [GAATTC] # 32 148 1733 2008 # # EcoRII: # [CCWGG] # 22 61 1865 1973 # # Esp3I: # [CGTCTC] # 1386 2535 # # FatI: # [CATG] # 313 459 638 669 902 940 # # FnuDII*: # [CGCG] # 349 372 935 2265 # # FokI: # [GGATG] # 4 27 108 110 116 118 # 119 120 177 180 194 196 # 243 283 378 408 1140 # # GlaI: # [GCGC] # 24 47 111 191 348 490 # 648 754 1308 # # GsuI: # [CTGGAG] # 255 537 1113 2016 # # HaeII: # [RGCGCY] # 24 646 754 887 1610 # # HaeIII: # [GGCC] # 6 55 354 574 1413 1519 # # HgaI: # [GACGC] # 28 338 1002 1265 1288 # # HgiAI*: # [GWGCWC] # 239 263 277 736 2406 # # HgiCI*: # [GGYRCC] # 489 579 679 1014 1160 # # HgiJII*: # [GRGCYC] # 277 1392 2252 # # HhaI: # [GCGC] # 24 47 111 191 349 490 # 647 754 1308 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 32 32 32 32 32 32 # 111 116 118 168 225 253 # 700 793 1181 # # Hin4II*: # [CCTTC] # 51 73 78 289 333 381 # 458 1058 1200 # # Hin6I: # [GCGC] # 24 47 111 191 347 490 # 649 754 1308 # # HindII: # [GTYRAC] # 106 974 2841 # # HindIII: # [AAGCTT] # 149 492 880 2400 # # HinfI: # [GANTC] # 75 83 95 105 106 120 # 144 193 298 320 504 813 # 1065 # # HpaI: # [GTTAAC] # 974 2947 # # HpaII: # [CCGG] # 18 21 57 102 102 433 # 631 1033 1524 # # HphI: # [GGTGA] # 132 222 484 749 903 1431 # # Hpy166II: # [GTNNAC] # 20 106 185 339 353 363 # 390 436 523 599 607 # # Hpy178III*: # [TCNNGA] # 11 14 15 36 46 46 # 82 127 128 153 157 220 # 394 418 2074 # # Hpy188I: # [TCNGA] # 19 28 30 33 39 48 # 51 51 51 51 51 51 # 57 75 79 99 102 107 # 108 117 117 132 132 198 # 211 271 290 309 480 534 # # Hpy99I: # [CGWCG] # 158 1113 1294 1356 # # Ksp632I*: # [CTCTTC] # 249 651 1341 1680 # # MaeI: # [CTAG] # 4 34 42 57 66 105 # 141 147 159 162 165 180 # 197 198 351 431 510 972 # # MaeII: # [ACGT] # 131 156 196 249 273 289 # 390 397 596 1244 # # MaeIII: # [GTNAC] # 9 10 116 180 334 435 # 495 2342 # # MboI: # [GATC] # 12 15 27 488 993 2386 # # MboII: # [GAAGA] # 6 36 39 72 117 160 # 173 225 236 336 466 714 # 1341 # # McrI*: # [CGRYCG] # 416 1111 2394 # # MfeI: # [CAATTG] # 40 101 906 2874 # # MlyI: # [GAGTC] # 101 129 645 1431 1615 # # MmeI: # [TCCRAC] # 11 27 35 109 180 298 # 314 325 341 452 1829 # # MnlI: # [CCTC] # 3 5 7 19 21 27 # 27 36 38 40 47 60 # 62 69 71 75 75 76 # 78 85 93 100 108 111 # 181 183 225 231 234 261 # 292 468 513 # # MseI: # [TTAA] # 39 105 232 329 644 793 # 1779 # # MslI: # [CAYNNNNRTG] # 306 688 1422 1505 # # MwoI: # [GCNNNNNNNGC] # 9 12 48 54 59 61 # 91 140 141 191 229 394 # 421 423 547 1101 # # NdeI: # [CATATG] # 300 3621 # # NheI: # [GCTAGC] # 4 35 162 198 216 263 # 925 2118 # # NlaIII: # [CATG] # 309 459 638 669 906 940 # # NlaIV: # [GGNNCC] # 38 226 315 408 484 489 # 528 681 752 # # NspBII*: # [CMGCKG] # 123 207 232 589 608 855 # 1307 # # OliI: # [CACNNNNGTG] # 306 1505 2110 # # PasI: # [CCCWGGG] # 21 3900 # # PleI: # [GAGTC] # 101 128 645 1432 1615 # # PspXI: # [VCTCGAGB] # 1269 2652 # # PsrI: # [GAACNNNNNNTAC] # 32 32 32 32 116 300 # 346 747 2284 # # PstI: # [CTGCAG] # 690 1422 1809 # # PvuII: # [CAGCTG] # 207 589 963 2162 # # RsaI: # [GTAC] # 30 42 51 64 81 142 # 158 168 183 195 204 304 # 311 319 378 411 429 451 # # SacI: # [GAGCTC] # 277 3644 # # SacII: # [CCGCGG] # 1306 2615 # # SauI*: # [CCTNAGG] # 916 1188 1817 # # ScaI: # [AGTACT] # 81 183 297 1053 2307 # # ScrFI: # [CCNGG] # 1 20 57 61 1809 1973 # # SduI: # [GDGCHC] # 154 239 263 277 736 2252 # # SecI*: # [CCNNGG] # 1 21 56 747 1287 1809 # # SetI: # [ASST] # 4 4 8 8 8 9 # 11 11 12 13 15 15 # 16 17 22 22 22 23 # 24 24 24 26 32 33 # 36 42 44 46 48 51 # 57 59 60 64 67 69 # 70 71 76 77 81 86 # 87 88 96 96 98 105 # 106 108 111 126 154 184 # 196 199 266 394 # # SfaNI: # [GCATC] # 9 99 106 116 177 204 # 258 341 381 420 536 589 # 685 # # SfeI*: # [CTRYAG] # 15 48 87 162 289 326 # 647 694 760 893 # # SmaI: # [CCCGGG] # 58 3863 # # SmlI: # [CTYRAG] # 51 51 51 51 51 51 # 60 194 339 405 410 685 # 1522 # # SpeI: # [ACTAGT] # 571 609 2741 # # SrfI: # [GCCCGGGC] # 58 3863 # # SspI: # [AATATT] # 419 3502 # # StuI: # [AGGCCT] # 1828 2093 # # TaiI: # [ACGT] # 131 156 196 249 273 289 # 390 394 599 1244 # # TaqI: # [TCGA] # 28 33 43 68 84 411 # 599 600 606 642 807 # # TatI: # [WGTACW] # 42 81 183 255 319 326 # 406 451 715 1143 # # TauI: # [GCSGC] # 369 913 935 1704 # # TfiI: # [GAWTC] # 83 106 170 313 320 442 # 504 813 1170 # # TseI: # [GCWGC] # 18 48 63 91 144 210 # 333 424 608 701 1281 # # TsoI: # [TARCCA] # 17 1347 2557 # # Tsp45I: # [GTSAC] # 10 116 939 2856 # # Tsp4CI*: # [ACNGT] # 25 49 104 213 247 351 # 768 1014 1150 # # TspDTI: # [ATGAA] # 32 45 57 103 132 146 # 147 161 178 223 246 476 # 802 1173 # # TspEI: # [AATT] # 7 12 17 18 23 32 # 33 33 38 40 56 80 # 89 101 151 188 199 236 # 316 451 834 967 # # TspGWI: # [ACGGA] # 51 51 106 379 486 517 # 2331 # # TspRI: # [CASTGNN] # 6 31 41 108 145 179 # 246 250 276 338 465 846 # 990 # # TstI: # [CACNNNNNNTCC] # 32 32 535 930 2392 # # XcmI: # [CCANNNNNNNNNTGG] # 174 3747 # # XhoI: # [CTCGAG] # 1269 2652 # # XhoII: # [RGATCY] # 12 15 27 488 3379 # # XmaIII*: # [CGGCCG] # 413 3508 # # XmnI: # [GAANNNNTTC] # 785 3136 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 3921 # Reported_sequences: 1 # Reported_hitcount: 1077 #---------------------------------------