Restriction Fragments for YGLWsigma2



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGLWsigma2 on chromosome VII from coordinates 401616 to 401894.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.22680.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 27 + BdaI TGANNNNNNTCA 5 3 39 37 16 27 - BdaI TGANNNNNNTCA 39 37 5 3 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 42 + Eam1105I GACNNNNNGTC 37 36 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 42 46 - FokI GGATG 28 32 . . 42 46 - BseGI GGATG 41 39 . . 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 53 57 - TspDTI ATGAA 43 41 . . 64 69 + NdeI CATATG 65 67 . . 67 71 + TspDTI ATGAA 82 80 . . 75 78 + SetI ASST 78 74 . . 96 99 + FatI CATG 95 99 . . 96 99 + NlaIII CATG 99 95 . . 96 99 + CviAII CATG 96 98 . . 108 112 + BssKI CCNGG 107 112 . . 108 112 + ScrFI CCNGG 109 110 . . 108 112 + CauII* CCSGG 109 110 . . 109 112 + HpaII CCGG 109 111 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + HaeIII GGCC 112 112 . . 113 117 + Hin4II* CCTTC 123 122 . . 119 122 + MaeI CTAG 119 121 . . 121 124 + SetI ASST 124 120 . . 121 124 + CviJI RGCY 122 122 . . 121 124 + AluI AGCT 122 122 . . 154 157 + TspEI AATT 153 157 . . 163 168 + XhoII RGATCY 163 167 . . 164 167 + DpnI GATC 165 165 . . 164 167 + BstKTI GATC 166 164 . . 164 167 + MboI GATC 163 167 . . 166 170 + Hpy188I TCNGA 168 167 . . 164 168 - BinI* GGATC 158 159 . . 171 175 + TfiI GAWTC 171 174 . . 171 175 + HinfI GANTC 171 174 . . 175 178 + AciI CCGC 175 177 . . 176 179 + FnuDII* CGCG 177 177 . . 177 180 + Hin6I GCGC 177 179 . . 177 180 + GlaI GCGC 178 178 . . 177 180 + HhaI GCGC 179 177 . . 189 193 + DdeI CTNAG 189 192 . . 197 201 + TfiI GAWTC 197 200 . . 197 201 + HinfI GANTC 197 200 . . 199 203 - TspDTI ATGAA 189 187 . . 227 231 + MaeIII GTNAC 226 231 . . 234 238 - TspGWI ACGGA 224 222 . . 240 243 + TspEI AATT 239 243 . . 241 246 + VspI ATTAAT 242 244 . . 242 245 + MseI TTAA 242 244 . . 246 250 + Hpy188I TCNGA 248 247 . . 254 258 + Tsp4CI* ACNGT 256 255 . . 266 270 + BsrI ACTGG 271 269 . . 270 274 + MaeIII GTNAC 269 274 . . 268 273 - TsoI TARCCA 258 256 . . #--------------------------------------- # # # AciI: # [CCGC] # 104 175 # # AclI: # [AACGTT] # 50 229 # # AluI: # [AGCT] # 122 157 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 240 # # BinI*: # [GGATC] # 121 158 # # BseGI: # [GGATG] # 41 238 # # BsrI: # [ACTGG] # 8 271 # # BssKI: # [CCNGG] # 107 172 # # BstKTI: # [GATC] # 113 166 # # CauII*: # [CCSGG] # 109 170 # # CviAII: # [CATG] # 96 183 # # CviJI: # [RGCY] # 10 112 157 # # DdeI: # [CTNAG] # 90 189 # # DpnI: # [GATC] # 114 165 # # Eam1105I: # [GACNNNNNGTC] # 37 242 # # FatI: # [CATG] # 95 184 # # FnuDII*: # [CGCG] # 102 177 # # FokI: # [GGATG] # 28 251 # # GlaI: # [GCGC] # 101 178 # # HaeIII: # [GGCC] # 112 167 # # HhaI: # [GCGC] # 100 179 # # Hin4I: # [GAYNNNNNVTC] # 0 0 23 32 224 # # Hin4II*: # [CCTTC] # 123 156 # # Hin6I: # [GCGC] # 102 177 # # HinfI: # [GANTC] # 26 82 171 # # HpaII: # [CCGG] # 109 170 # # Hpy188I: # [TCNGA] # 31 80 168 # # MaeI: # [CTAG] # 119 160 # # MaeII: # [ACGT] # 12 38 229 # # MaeIII: # [GTNAC] # 10 43 226 # # MboI: # [GATC] # 116 163 # # MseI: # [TTAA] # 37 242 # # NdeI: # [CATATG] # 65 214 # # NlaIII: # [CATG] # 99 180 # # ScrFI: # [CCNGG] # 109 170 # # SetI: # [ASST] # 12 25 41 46 155 # # TaiI: # [ACGT] # 12 41 226 # # TfiI: # [GAWTC] # 26 82 171 # # TsoI: # [TARCCA] # 21 258 # # Tsp4CI*: # [ACNGT] # 23 256 # # TspDTI: # [ATGAA] # 39 43 90 107 # # TspEI: # [AATT] # 40 86 153 # # TspGWI: # [ACGGA] # 55 224 # # VspI: # [ATTAAT] # 37 242 # # XhoII: # [RGATCY] # 116 163 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 279 # Reported_sequences: 1 # Reported_hitcount: 60 #---------------------------------------