Restriction Fragments for YGLWdelta3



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGLWdelta3 on chromosome VII from coordinates 114939 to 115269.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30025.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 39 43 + Tsp45I GTSAC 38 43 . . 39 43 + MaeIII GTNAC 38 43 . . 48 52 - BsrI ACTGG 48 46 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 86 89 + SetI ASST 89 85 . . 97 101 + Tsp4CI* ACNGT 99 98 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 104 107 + TaqI TCGA 104 106 . . 113 116 - MnlI CCTC 106 105 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 120 125 + Eco57MI CTGRAG 141 139 . . 120 125 + Eco57I CTGAAG 141 139 . . 126 129 + CviRI* TGCA 127 127 . . 153 157 + TspDTI ATGAA 168 166 . . 167 171 + TspGWI ACGGA 182 180 . . 174 177 - MnlI CCTC 167 166 . . 174 184 - Hin4I GAYNNNNNVTC 197 192 165 160 190 195 + SspI AATATT 192 192 . . 213 223 + BsiYI* CCNNNNNNNGG 219 216 . . 220 230 + BplI GAGNNNNNCTC 211 206 243 238 223 227 + TfiI GAWTC 223 226 . . 220 223 - MnlI CCTC 213 212 . . 223 227 + HinfI GANTC 223 226 . . 227 230 + MnlI CCTC 237 236 . . 220 230 - BplI GAGNNNNNCTC 243 238 211 206 234 241 + PspXI VCTCGAGB 235 239 . . 234 237 + MnlI CCTC 244 243 . . 234 241 + AbsI CCTCGAGG 235 239 . . 235 240 + SmlI CTYRAG 235 239 . . 235 240 + XhoI CTCGAG 235 239 . . 235 240 + BmeT110I CYCGRG 236 238 . . 235 240 + AvaI CYCGRG 235 239 . . 236 239 + TaqI TCGA 236 238 . . 238 243 + BseRI GAGGAG 253 251 . . 238 241 - MnlI CCTC 231 230 . . 261 266 + Hpy166II GTNNAC 263 263 . . 261 266 + BssNAI GTATAC 263 263 . . 261 266 + AccI GTMKAC 262 264 . . 269 274 + SspI AATATT 271 271 . . 277 280 + CviJI RGCY 278 278 . . 282 287 + BclI TGATCA 282 286 . . 283 286 + DpnI GATC 284 284 . . 283 286 + BstKTI GATC 285 283 . . 283 286 + MboI GATC 282 286 . . 291 295 + TspDTI ATGAA 306 304 . . 311 314 + MseI TTAA 311 313 . . 314 319 + ApoI RAATTY 314 318 . . 315 318 + TspEI AATT 314 318 . . 318 322 - HphI GGTGA 310 309 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 96 235 # # AccI: # [GTMKAC] # 69 262 # # AluI: # [AGCT] # 119 212 # # ApoI: # [RAATTY] # 17 314 # # AvaI: # [CYCGRG] # 96 235 # # BclI: # [TGATCA] # 49 282 # # BmeT110I: # [CYCGRG] # 95 236 # # BplI: # [GAGNNNNNCTC] # 0 0 32 88 211 # # BseRI: # [GAGGAG] # 78 253 # # BsiYI*: # [CCNNNNNNNGG] # 112 219 # # BsrI: # [ACTGG] # 48 283 # # BssNAI: # [GTATAC] # 68 263 # # BstKTI: # [GATC] # 46 285 # # CviJI: # [RGCY] # 53 119 159 # # CviRI*: # [TGCA] # 127 204 # # DpnI: # [GATC] # 47 284 # # Eco57I: # [CTGAAG] # 141 190 # # Eco57MI: # [CTGRAG] # 141 190 # # Hin4I: # [GAYNNNNNVTC] # 32 32 50 83 134 # # HinfI: # [GANTC] # 108 223 # # HphI: # [GGTGA] # 21 310 # # Hpy166II: # [GTNNAC] # 68 263 # # MaeI: # [CTAG] # 33 298 # # MaeIII: # [GTNAC] # 38 293 # # MboI: # [GATC] # 49 282 # # MnlI: # [CCTC] # 6 7 18 46 61 87 # 106 # # MseI: # [TTAA] # 20 311 # # PspXI: # [VCTCGAGB] # 96 235 # # SetI: # [ASST] # 32 89 210 # # SmlI: # [CTYRAG] # 96 235 # # SpeI: # [ACTAGT] # 32 299 # # SspI: # [AATATT] # 60 79 192 # # TaqI: # [TCGA] # 95 104 132 # # TfiI: # [GAWTC] # 108 223 # # Tsp45I: # [GTSAC] # 38 293 # # Tsp4CI*: # [ACNGT] # 32 67 232 # # TspDTI: # [ATGAA] # 25 138 168 # # TspEI: # [AATT] # 17 314 # # TspGWI: # [ACGGA] # 149 182 # # XhoI: # [CTCGAG] # 96 235 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 331 # Reported_sequences: 1 # Reported_hitcount: 53 #---------------------------------------