Restriction Fragments for YGLCtau3



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YGLCtau3 on chromosome VII from coordinates 320707 to 320355.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.23009.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 12 15 - AciI CCGC 12 14 . . 10 15 - BsrBI CCGCTC 12 12 . . 19 22 + MseI TTAA 19 21 . . 25 29 + MaeIII GTNAC 24 29 . . 29 32 + FatI CATG 28 32 . . 29 32 + NlaIII CATG 32 28 . . 29 32 + CviAII CATG 29 31 . . 34 38 + MaeIII GTNAC 33 38 . . 43 47 + MaeIII GTNAC 42 47 . . 48 51 + DpnI GATC 49 49 . . 48 51 + BstKTI GATC 50 48 . . 48 51 + MboI GATC 47 51 . . 58 63 + AhaIII* TTTAAA 60 60 . . 59 62 + MseI TTAA 59 61 . . 73 77 + TspDTI ATGAA 88 86 . . 83 95 + BarI GAAGNNNNNNTAC 75 70 107 102 114 119 + NspI RCATGY 118 114 . . 115 118 + FatI CATG 114 118 . . 115 118 + NlaIII CATG 118 114 . . 115 118 + CviAII CATG 115 117 . . 119 123 + Hpy188I TCNGA 121 120 . . 125 130 + TatI WGTACW 125 129 . . 125 130 + ScaI AGTACT 127 127 . . 126 129 + Csp6I GTAC 126 128 . . 126 129 + RsaI GTAC 127 127 . . 131 136 + ApoI RAATTY 131 135 . . 132 135 + TspEI AATT 131 135 . . 140 143 + MseI TTAA 140 142 . . 144 148 + PleI GAGTC 152 153 . . 144 148 + MlyI GAGTC 153 153 . . 144 148 + HinfI GANTC 144 147 . . 150 155 + VspI ATTAAT 151 153 . . 151 154 + MseI TTAA 151 153 . . 153 156 + TspEI AATT 152 156 . . 162 167 + XhoII RGATCY 162 166 . . 162 167 + BglII AGATCT 162 166 . . 163 166 + DpnI GATC 164 164 . . 163 166 + BstKTI GATC 165 163 . . 163 166 + MboI GATC 162 166 . . 165 169 + Hpy188I TCNGA 167 166 . . 172 176 + Tsp45I GTSAC 171 176 . . 172 176 + MaeIII GTNAC 171 176 . . 192 196 + PleI GAGTC 200 201 . . 192 196 + MlyI GAGTC 201 201 . . 192 196 + HinfI GANTC 192 195 . . 197 201 + Tsp45I GTSAC 196 201 . . 197 201 + MaeIII GTNAC 196 201 . . 203 206 + MseI TTAA 203 205 . . 211 215 + TspDTI ATGAA 226 224 . . 215 218 + SetI ASST 218 214 . . 215 218 + CviJI RGCY 216 216 . . 215 218 + AluI AGCT 216 216 . . 216 220 + BlsI GCNGC 218 217 . . 216 220 + BisI GCNGC 217 218 . . 216 220 + TseI GCWGC 216 219 . . 220 223 + HpaII CCGG 220 222 . . 216 220 - BbvI GCAGC 203 207 . . 223 227 + TfiI GAWTC 223 226 . . 223 227 + HinfI GANTC 223 226 . . 226 230 - HphI GGTGA 218 217 . . 238 243 + TsoI TARCCA 254 252 . . 239 242 + CviJI RGCY 240 240 . . 253 257 + TspDTI ATGAA 268 266 . . 265 270 + NspI RCATGY 269 265 . . 266 269 + FatI CATG 265 269 . . 266 269 + NlaIII CATG 269 265 . . 266 269 + CviAII CATG 266 268 . . 278 281 + SetI ASST 281 277 . . 279 282 + MnlI CCTC 289 288 . . 292 297 + TatI WGTACW 292 296 . . 293 296 + Csp6I GTAC 293 295 . . 293 296 + RsaI GTAC 294 294 . . 301 306 + XhoII RGATCY 301 305 . . 302 305 + DpnI GATC 303 303 . . 302 305 + BstKTI GATC 304 302 . . 302 305 + MboI GATC 301 305 . . 302 306 - BinI* GGATC 296 297 . . 310 314 - TspDTI ATGAA 300 298 . . 315 319 - MboII GAAGA 307 306 . . 320 325 + VspI ATTAAT 321 323 . . 317 321 - TspDTI ATGAA 307 305 . . 321 324 + MseI TTAA 321 323 . . 329 332 + SetI ASST 332 328 . . 333 338 + Hpy166II GTNNAC 335 335 . . 333 338 + HpaI GTTAAC 335 335 . . 333 338 + HindII GTYRAC 335 335 . . 334 337 + MseI TTAA 334 336 . . 341 344 + MseI TTAA 341 343 . . #--------------------------------------- # # # AciI: # [CCGC] # 12 341 # # AhaIII*: # [TTTAAA] # 60 293 # # AluI: # [AGCT] # 137 216 # # ApoI: # [RAATTY] # 131 222 # # BarI: # [GAAGNNNNNNTAC] # 32 75 246 # # BbvI: # [GCAGC] # 150 203 # # BglII: # [AGATCT] # 162 191 # # BinI*: # [GGATC] # 57 296 # # BisI: # [GCNGC] # 136 217 # # BlsI: # [GCNGC] # 135 218 # # BsrBI: # [CCGCTC] # 12 341 # # BstKTI: # [GATC] # 49 50 115 139 # # Csp6I: # [GTAC] # 60 126 167 # # CviAII: # [CATG] # 29 86 87 151 # # CviJI: # [RGCY] # 24 113 216 # # DpnI: # [GATC] # 49 50 115 139 # # FatI: # [CATG] # 28 86 88 151 # # HindII: # [GTYRAC] # 18 335 # # HinfI: # [GANTC] # 31 48 130 144 # # HpaI: # [GTTAAC] # 18 335 # # HpaII: # [CCGG] # 133 220 # # HphI: # [GGTGA] # 135 218 # # Hpy166II: # [GTNNAC] # 18 335 # # Hpy188I: # [TCNGA] # 46 121 186 # # MaeIII: # [GTNAC] # 9 9 24 25 129 157 # # MboI: # [GATC] # 47 52 115 139 # # MboII: # [GAAGA] # 46 307 # # MlyI: # [GAGTC] # 48 152 153 # # MnlI: # [CCTC] # 64 289 # # MseI: # [TTAA] # 7 11 12 13 19 40 # 52 81 118 # # NlaIII: # [CATG] # 32 84 86 151 # # NspI: # [RCATGY] # 84 118 151 # # PleI: # [GAGTC] # 48 152 153 # # RsaI: # [GTAC] # 59 127 167 # # ScaI: # [AGTACT] # 127 226 # # SetI: # [ASST] # 21 51 63 218 # # TatI: # [WGTACW] # 61 125 167 # # TfiI: # [GAWTC] # 130 223 # # TseI: # [GCWGC] # 137 216 # # TsoI: # [TARCCA] # 99 254 # # Tsp45I: # [GTSAC] # 25 157 171 # # TspDTI: # [ATGAA] # 7 32 42 46 88 138 # # TspEI: # [AATT] # 21 131 201 # # VspI: # [ATTAAT] # 32 151 170 # # XhoII: # [RGATCY] # 52 139 162 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 353 # Reported_sequences: 1 # Reported_hitcount: 88 #---------------------------------------