Restriction Fragments for ZRT1/YGL255W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

ZRT1/YGL255W on chromosome VII from coordinates 20978 to 22108.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.28386.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 12 + AclI AACGTT 8 10 . . 8 11 + TaiI ACGT 11 7 . . 8 11 + SetI ASST 11 7 . . 8 11 + MaeII ACGT 8 10 . . 10 14 + MaeIII GTNAC 9 14 . . 19 24 + DsaI* CCRYGG 19 23 . . 19 24 + SecI* CCNNGG 19 23 . . 18 22 - BceAI ACGGC 3 5 . . 35 41 + SanDI GGGWCCC 36 39 . . 35 41 + PpuMI RGGWCCY 36 39 . . 35 41 + DraII RGGNCCY 36 39 . . 35 39 + BslFI GGGAC 49 53 . . 35 40 + NlaIV GGNNCC 37 37 . . 36 41 + NlaIV GGNNCC 38 38 . . 36 40 + BmgT120I GGNCC 37 38 . . 36 40 + AvaII GGWCC 36 39 . . 36 40 + AsuI* GGNCC 36 39 . . 40 44 + Hin4II* CCTTC 50 49 . . 43 47 + Hpy188I TCNGA 45 44 . . 44 49 + Eco57MI CTGRAG 65 63 . . 44 49 + Eco57I CTGAAG 65 63 . . 47 57 + FalI AAGNNNNNCTT 38 33 70 65 49 53 + MaeIII GTNAC 48 53 . . 47 57 - FalI AAGNNNNNCTT 70 65 38 33 84 88 + MboII GAAGA 96 95 . . 84 89 + BbvII* GAAGAC 91 95 . . 88 91 + SetI ASST 91 87 . . 83 94 - TstI CACNNNNNNTCC 107 102 75 70 98 101 + CviRI* TGCA 99 99 . . 102 105 + SetI ASST 105 101 . . 106 111 + Hpy166II GTNNAC 108 108 . . 125 130 + TatI WGTACW 125 129 . . 126 129 + Csp6I GTAC 126 128 . . 126 129 + RsaI GTAC 127 127 . . 134 138 + MaeIII GTNAC 133 138 . . 138 142 + DdeI CTNAG 138 141 . . 141 144 + SetI ASST 144 140 . . 142 147 + NlaIV GGNNCC 144 144 . . 142 147 + HgiCI* GGYRCC 142 146 . . 149 158 + XmnI GAANNNNTTC 153 153 . . 154 158 - MboII GAAGA 146 145 . . 157 161 - TspGWI ACGGA 147 145 . . 176 181 + Hpy178III* TCNNGA 177 179 . . 181 186 + TatI WGTACW 181 185 . . 181 186 + ScaI AGTACT 183 183 . . 182 185 + Csp6I GTAC 182 184 . . 182 185 + RsaI GTAC 183 183 . . 195 198 + FatI CATG 194 198 . . 195 198 + NlaIII CATG 198 194 . . 195 198 + CviAII CATG 195 197 . . 191 195 - HphI GGTGA 183 182 . . 204 209 + VspI ATTAAT 205 207 . . 205 208 + MseI TTAA 205 207 . . 231 241 + BplI GAGNNNNNCTC 222 217 254 249 233 238 + EcoRI GAATTC 233 237 . . 233 238 + ApoI RAATTY 233 237 . . 234 237 + TspEI AATT 233 237 . . 238 241 + MnlI CCTC 248 247 . . 231 241 - BplI GAGNNNNNCTC 254 249 222 217 247 252 + Hpy166II GTNNAC 249 249 . . 251 254 + SetI ASST 254 250 . . 271 276 + NlaIV GGNNCC 273 273 . . 274 279 + BetI* WCCGGW 274 278 . . 275 278 + HpaII CCGG 275 277 . . 288 291 + CviRI* TGCA 289 289 . . 293 296 + AciI CCGC 293 295 . . 303 313 + BsiYI* CCNNNNNNNGG 309 306 . . 303 313 + PflMI CCANNNNNTGG 309 306 . . 307 310 + MseI TTAA 307 309 . . 312 317 + NlaIV GGNNCC 314 314 . . 312 316 + BmgT120I GGNCC 313 314 . . 312 316 + AvaII GGWCC 312 315 . . 312 316 + AsuI* GGNCC 312 315 . . 316 326 + BsiYI* CCNNNNNNNGG 322 319 . . 319 329 + MwoI GCNNNNNNNGC 325 322 . . 337 342 + KpnI GGTACC 341 337 . . 337 342 + NlaIV GGNNCC 339 339 . . 337 342 + HgiCI* GGYRCC 337 341 . . 337 342 + Acc65I GGTACC 337 341 . . 338 341 + Csp6I GTAC 338 340 . . 338 341 + RsaI GTAC 339 339 . . 358 363 + Cfr10I RCCGGY 358 362 . . 358 363 + BetI* WCCGGW 358 362 . . 358 363 + AgeI ACCGGT 358 362 . . 359 362 + HpaII CCGG 359 361 . . 359 369 + BsiYI* CCNNNNNNNGG 365 362 . . 362 366 + MaeIII GTNAC 361 366 . . 365 369 + BsrI ACTGG 370 368 . . 365 370 + BfiI ACTGGG 375 374 . . 380 383 + FatI CATG 379 383 . . 380 383 + NlaIII CATG 383 379 . . 380 383 + CviAII CATG 380 382 . . 378 382 - TspDTI ATGAA 368 366 . . 412 415 + SetI ASST 415 411 . . 413 417 + Hin4II* CCTTC 423 422 . . 430 433 + DpnI GATC 431 431 . . 430 433 + BstKTI GATC 432 430 . . 430 433 + MboI GATC 429 433 . . 443 447 - HgaI GACGC 432 437 . . 475 478 + FatI CATG 474 478 . . 475 478 + NlaIII CATG 478 474 . . 475 478 + CviAII CATG 475 477 . . 491 495 + TspDTI ATGAA 506 504 . . 495 498 + TspEI AATT 494 498 . . 497 500 + MseI TTAA 497 499 . . 504 510 + TspRI CASTGNN 510 501 . . 504 513 + MslI CAYNNNNRTG 508 508 . . 504 513 + OliI CACNNNNGTG 508 508 . . 505 509 + Tsp4CI* ACNGT 507 506 . . 519 525 + TspRI CASTGNN 525 516 . . 521 526 + PstI CTGCAG 525 521 . . 521 526 + SfeI* CTRYAG 521 525 . . 522 525 + CviRI* TGCA 523 523 . . 523 527 + BlsI GCNGC 525 524 . . 523 527 + BisI GCNGC 524 525 . . 523 527 + BbvI GCAGC 535 539 . . 523 527 + TseI GCWGC 523 526 . . 524 529 + PvuII CAGCTG 526 526 . . 524 529 + NspBII* CMGCKG 526 526 . . 519 524 - BtsI GCAGTG 518 516 . . 525 528 + SetI ASST 528 524 . . 525 528 + CviJI RGCY 526 526 . . 525 528 + AluI AGCT 526 526 . . 557 560 + Csp6I GTAC 557 559 . . 557 560 + RsaI GTAC 558 558 . . 561 564 + CviRI* TGCA 562 562 . . 568 573 + XhoII RGATCY 568 572 . . 568 572 + BinI* GGATC 576 577 . . 569 572 + DpnI GATC 570 570 . . 569 572 + BstKTI GATC 571 569 . . 569 572 + MboI GATC 568 572 . . 573 578 + Hpy178III* TCNNGA 574 576 . . 573 578 + BspHI TCATGA 573 577 . . 574 577 + FatI CATG 573 577 . . 574 577 + NlaIII CATG 577 573 . . 574 577 + CviAII CATG 574 576 . . 587 591 + TspGWI ACGGA 602 600 . . 602 606 + TspDTI ATGAA 617 615 . . 604 608 + MboII GAAGA 616 615 . . 607 611 + TfiI GAWTC 607 610 . . 607 611 + HinfI GANTC 607 610 . . 610 614 + Hpy188I TCNGA 612 611 . . 613 623 + Hin4I GAYNNNNNVTC 604 599 636 631 613 617 + HgaI GACGC 622 627 . . 613 623 - Hin4I GAYNNNNNVTC 636 631 604 599 623 628 + DsaI* CCRYGG 623 627 . . 623 628 + StyI CCWWGG 623 627 . . 623 628 + NcoI CCATGG 623 627 . . 623 628 + SecI* CCNNGG 623 627 . . 620 624 - FokI GGATG 606 610 . . 624 627 + FatI CATG 623 627 . . 624 627 + NlaIII CATG 627 623 . . 624 627 + CviAII CATG 624 626 . . 620 624 - BseGI GGATG 619 617 . . 627 631 + FokI GGATG 640 644 . . 627 631 + BseGI GGATG 633 631 . . 653 656 + TspEI AATT 652 656 . . 667 670 + MseI TTAA 667 669 . . 669 672 + TspEI AATT 668 672 . . 676 681 + EcoRI GAATTC 676 680 . . 676 681 + ApoI RAATTY 676 680 . . 677 680 + TspEI AATT 676 680 . . 697 701 - TspGWI ACGGA 687 685 . . 705 708 + DpnI GATC 706 706 . . 705 708 + BstKTI GATC 707 705 . . 705 708 + MboI GATC 704 708 . . 707 712 - TaqII GACCGA 697 695 . . 716 719 + SetI ASST 719 715 . . 717 721 + BssKI CCNGG 716 721 . . 717 721 + BseBI CCWGG 718 719 . . 717 722 + SecI* CCNNGG 717 721 . . 717 721 + ScrFI CCNGG 718 719 . . 717 721 + EcoRII CCWGG 716 721 . . 730 734 + HphI GGTGA 742 741 . . 733 743 + Hin4I GAYNNNNNVTC 724 719 756 751 736 746 + BplI GAGNNNNNCTC 727 722 759 754 743 746 + MnlI CCTC 753 752 . . 741 746 - BseRI GAGGAG 732 730 . . 736 746 - BplI GAGNNNNNCTC 759 754 727 722 759 763 + DdeI CTNAG 759 762 . . 768 772 + BccI CCATC 776 777 . . 783 786 + SetI ASST 786 782 . . 781 785 - Hin4II* CCTTC 775 774 . . 789 792 + SetI ASST 792 788 . . 798 801 + CviRI* TGCA 799 799 . . 810 813 + CviJI RGCY 811 811 . . 817 822 + EcoRI GAATTC 817 821 . . 817 822 + ApoI RAATTY 817 821 . . 818 821 + TspEI AATT 817 821 . . 824 827 + MaeI CTAG 824 826 . . 827 830 + DpnI GATC 828 828 . . 827 830 + BstKTI GATC 829 827 . . 827 830 + MboI GATC 826 830 . . 836 840 - BccI CCATC 830 831 . . 841 846 + BalI TGGCCA 843 843 . . 841 846 + CfrI YGGCCR 841 845 . . 842 845 + CviJI RGCY 843 843 . . 842 845 + HaeIII GGCC 843 843 . . 844 849 + DsaI* CCRYGG 844 848 . . 844 849 + StyI CCWWGG 844 848 . . 844 849 + NcoI CCATGG 844 848 . . 844 849 + SecI* CCNNGG 844 848 . . 845 848 + FatI CATG 844 848 . . 845 848 + NlaIII CATG 848 844 . . 845 848 + CviAII CATG 845 847 . . 848 854 + DraII RGGNCCY 849 852 . . 848 853 + SduI GDGCHC 852 848 . . 848 853 + Bsp120I GGGCCC 848 852 . . 848 853 + NlaIV GGNNCC 850 850 . . 848 852 + BmgT120I GGNCC 849 850 . . 848 853 + HgiJII* GRGCYC 852 848 . . 848 853 + BseSI GKGCMC 852 848 . . 848 852 + AsuI* GGNCC 848 851 . . 848 853 + ApaI GGGCCC 852 848 . . 849 852 + CviJI RGCY 850 850 . . 849 852 + HaeIII GGCC 850 850 . . 849 853 + BmgT120I GGNCC 850 851 . . 849 853 + AsuI* GGNCC 849 852 . . 870 875 + Hpy166II GTNNAC 872 872 . . 870 875 + HpaI GTTAAC 872 872 . . 870 875 + HindII GTYRAC 872 872 . . 871 874 + MseI TTAA 871 873 . . 872 877 + TsoI TARCCA 888 886 . . 881 892 + CspCI CAANNNNNGTGG 869 867 904 902 890 895 + BalI TGGCCA 892 892 . . 890 895 + CfrI YGGCCR 890 894 . . 891 894 + CviJI RGCY 892 892 . . 891 894 + HaeIII GGCC 892 892 . . 893 904 + BstXI CCANNNNNNTGG 900 896 . . 893 897 + BccI CCATC 901 902 . . 902 907 - TaqII CACCCA 892 890 . . 911 914 + Csp6I GTAC 911 913 . . 911 914 + RsaI GTAC 912 912 . . 920 923 + TaiI ACGT 923 919 . . 920 923 + SetI ASST 923 919 . . 920 923 + MaeII ACGT 920 922 . . 924 929 + NspBII* CMGCKG 926 926 . . 926 929 - AciI CCGC 926 928 . . 936 942 + TspRI CASTGNN 942 933 . . 940 943 + Hin6I GCGC 940 942 . . 940 943 + GlaI GCGC 941 941 . . 940 943 + HhaI GCGC 942 940 . . 936 941 - BtsI GCAGTG 935 933 . . 963 967 + FokI GGATG 976 980 . . 963 967 + BseGI GGATG 969 967 . . 967 977 + MwoI GCNNNNNNNGC 973 970 . . 964 968 - SfaNI GCATC 954 958 . . 974 979 - EcoP15I CAGCAG 946 948 . . 989 994 + TatI WGTACW 989 993 . . 989 994 + Bsp1407I TGTACA 989 993 . . 990 995 + Hpy166II GTNNAC 992 992 . . 990 993 + Csp6I GTAC 990 992 . . 990 993 + RsaI GTAC 991 991 . . 993 999 + TspRI CASTGNN 999 990 . . 994 998 + BsrI ACTGG 999 997 . . 1012 1015 + MaeI CTAG 1012 1014 . . 1019 1023 - BsmAI GTCTC 1013 1017 . . 1036 1039 + MnlI CCTC 1046 1045 . . 1050 1055 + XhoII RGATCY 1050 1054 . . 1050 1054 + BinI* GGATC 1058 1059 . . 1051 1054 + DpnI GATC 1052 1052 . . 1051 1054 + BstKTI GATC 1053 1051 . . 1051 1054 + MboI GATC 1050 1054 . . 1054 1058 + DdeI CTNAG 1054 1057 . . 1061 1064 + TspEI AATT 1060 1064 . . 1067 1071 + Hin4II* CCTTC 1077 1076 . . 1072 1077 + AclI AACGTT 1073 1075 . . 1073 1076 + TaiI ACGT 1076 1072 . . 1073 1076 + SetI ASST 1076 1072 . . 1073 1076 + MaeII ACGT 1073 1075 . . 1081 1084 + CviRI* TGCA 1082 1082 . . 1082 1093 - BcgI CGANNNNNNTGC 1105 1103 1071 1069 1104 1107 + FatI CATG 1103 1107 . . 1104 1107 + NlaIII CATG 1107 1103 . . 1104 1107 + CviAII CATG 1104 1106 . . 1107 1110 + CviJI RGCY 1108 1108 . . 1113 1116 + DpnI GATC 1114 1114 . . 1113 1116 + BstKTI GATC 1115 1113 . . 1113 1116 + MboI GATC 1112 1116 . . 1125 1128 + CviJI RGCY 1126 1126 . . 1128 1131 + MseI TTAA 1128 1130 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 337 794 # # AciI: # [CCGC] # 205 293 633 # # AclI: # [AACGTT] # 8 58 1065 # # AgeI: # [ACCGGT] # 358 773 # # AluI: # [AGCT] # 526 605 # # ApaI: # [GGGCCC] # 279 852 # # ApoI: # [RAATTY] # 141 233 314 443 # # AsuI*: # [GGNCC] # 1 36 276 282 536 # # AvaII: # [GGWCC] # 36 276 819 # # BalI: # [TGGCCA] # 49 239 843 # # BbvI: # [GCAGC] # 535 596 # # BbvII*: # [GAAGAC] # 91 1040 # # BccI: # [CCATC] # 54 71 230 776 # # BceAI: # [ACGGC] # 3 1128 # # BcgI: # [CGANNNNNNTGC] # 26 34 1071 # # BetI*: # [WCCGGW] # 84 274 773 # # BfiI: # [ACTGGG] # 375 756 # # BinI*: # [GGATC] # 73 482 576 # # BisI: # [GCNGC] # 524 607 # # BlsI: # [GCNGC] # 525 606 # # BmgT120I: # [GGNCC] # 1 37 276 281 536 # # BplI: # [GAGNNNNNCTC] # 0 0 0 0 32 32 # 222 372 473 # # BseBI: # [CCWGG] # 413 718 # # BseGI: # [GGATG] # 14 162 336 619 # # BseRI: # [GAGGAG] # 399 732 # # BseSI: # [GKGCMC] # 279 852 # # BsiYI*: # [CCNNNNNNNGG] # 13 43 309 766 # # BslFI: # [GGGAC] # 49 1082 # # BsmAI: # [GTCTC] # 118 1013 # # Bsp120I: # [GGGCCC] # 283 848 # # Bsp1407I: # [TGTACA] # 142 989 # # BspHI: # [TCATGA] # 558 573 # # BsrI: # [ACTGG] # 132 370 629 # # BssKI: # [CCNGG] # 415 716 # # BstKTI: # [GATC] # 16 62 122 136 139 224 # 432 # # BstXI: # [CCANNNNNNTGG] # 231 900 # # BtsI: # [GCAGTG] # 196 417 518 # # Cfr10I: # [RCCGGY] # 358 773 # # CfrI: # [YGGCCR] # 49 241 841 # # Csp6I: # [GTAC] # 56 79 126 141 156 219 # 354 # # CspCI: # [CAANNNNNGTGG] # 35 227 869 # # CviAII: # [CATG] # 27 50 95 99 185 195 # 221 259 # # CviJI: # [RGCY] # 5 7 18 32 42 216 # 285 526 # # CviRI*: # [TGCA] # 39 49 99 190 234 237 # 283 # # DdeI: # [CTNAG] # 77 138 295 621 # # DpnI: # [GATC] # 17 62 122 136 139 224 # 431 # # DraII: # [RGGNCCY] # 36 282 813 # # DsaI*: # [CCRYGG] # 19 221 287 604 # # Eco57I: # [CTGAAG] # 65 1066 # # Eco57MI: # [CTGRAG] # 65 1066 # # EcoP15I: # [CAGCAG] # 185 946 # # EcoRI: # [GAATTC] # 141 233 314 443 # # EcoRII: # [CCWGG] # 415 716 # # FalI: # [AAGNNNNNCTT] # 0 0 32 38 1061 # # FatI: # [CATG] # 28 50 95 99 185 194 # 221 259 # # FokI: # [GGATG] # 34 155 336 606 # # GlaI: # [GCGC] # 190 941 # # HaeIII: # [GGCC] # 7 42 239 843 # # HgaI: # [GACGC] # 190 432 509 # # HgiCI*: # [GGYRCC] # 142 195 794 # # HgiJII*: # [GRGCYC] # 279 852 # # HhaI: # [GCGC] # 189 942 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 88 375 # 604 # # Hin4II*: # [CCTTC] # 50 54 302 352 373 # # Hin6I: # [GCGC] # 191 940 # # HindII: # [GTYRAC] # 259 872 # # HinfI: # [GANTC] # 524 607 # # HpaI: # [GTTAAC] # 259 872 # # HpaII: # [CCGG] # 84 275 772 # # HphI: # [GGTGA] # 183 389 559 # # Hpy166II: # [GTNNAC] # 108 120 139 141 623 # # Hpy178III*: # [TCNNGA] # 177 397 557 # # Hpy188I: # [TCNGA] # 45 519 567 # # KpnI: # [GGTACC] # 341 790 # # MaeI: # [CTAG] # 119 188 824 # # MaeII: # [ACGT] # 8 58 153 912 # # MaeIII: # [GTNAC] # 9 39 85 228 770 # # MboI: # [GATC] # 19 62 122 136 139 224 # 429 # # MboII: # [GAAGA] # 50 96 470 515 # # MnlI: # [CCTC] # 85 248 293 505 # # MseI: # [TTAA] # 3 102 170 190 204 205 # 257 # # MslI: # [CAYNNNNRTG] # 508 623 # # MwoI: # [GCNNNNNNNGC] # 158 325 648 # # NcoI: # [CCATGG] # 221 287 623 # # NlaIII: # [CATG] # 24 50 95 99 185 198 # 221 259 # # NlaIV: # [GGNNCC] # 1 25 37 41 106 129 # 281 511 # # NspBII*: # [CMGCKG] # 205 400 526 # # OliI: # [CACNNNNGTG] # 508 623 # # PflMI: # [CCANNNNNTGG] # 309 822 # # PpuMI: # [RGGWCCY] # 36 1095 # # PstI: # [CTGCAG] # 525 606 # # PvuII: # [CAGCTG] # 526 605 # # RsaI: # [GTAC] # 56 79 127 140 156 219 # 354 # # SanDI: # [GGGWCCC] # 36 1095 # # ScaI: # [AGTACT] # 183 948 # # ScrFI: # [CCNGG] # 413 718 # # SduI: # [GDGCHC] # 279 852 # # SecI*: # [CCNNGG] # 19 94 127 287 604 # # SetI: # [ASST] # 6 11 14 39 55 67 # 80 110 113 131 153 161 # 191 # # SfaNI: # [GCATC] # 177 954 # # SfeI*: # [CTRYAG] # 521 610 # # StyI: # [CCWWGG] # 221 287 623 # # TaiI: # [ACGT] # 11 55 153 912 # # TaqII: # [GACCGA] # 195 239 697 # # TatI: # [WGTACW] # 56 125 142 808 # # TfiI: # [GAWTC] # 524 607 # # TseI: # [GCWGC] # 523 608 # # TsoI: # [TARCCA] # 243 888 # # Tsp4CI*: # [ACNGT] # 507 624 # # TspDTI: # [ATGAA] # 111 138 368 514 # # TspEI: # [AATT] # 8 16 71 141 158 233 # 243 261 # # TspGWI: # [ACGGA] # 85 147 444 455 # # TspRI: # [CASTGNN] # 15 57 132 417 510 # # TstI: # [CACNNNNNNTCC] # 32 75 1024 # # VspI: # [ATTAAT] # 205 926 # # XhoII: # [RGATCY] # 81 482 568 # # XmnI: # [GAANNNNTTC] # 153 978 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1131 # Reported_sequences: 1 # Reported_hitcount: 281 #---------------------------------------