Restriction Fragments for SUT1/YGL162W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SUT1/YGL162W on chromosome VII from coordinates 198138 to 199037.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.30967.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 16 20 + Tsp4CI* ACNGT 18 17 . . 31 34 + DpnI GATC 32 32 . . 31 34 + BstKTI GATC 33 31 . . 31 34 + MboI GATC 30 34 . . 32 37 + ClaI ATCGAT 33 35 . . 33 36 + TaqI TCGA 33 35 . . 34 39 + McrI* CGRYCG 37 35 . . 34 39 + PvuI CGATCG 37 35 . . 35 38 + DpnI GATC 36 36 . . 35 38 + BstKTI GATC 37 35 . . 35 38 + MboI GATC 34 38 . . 39 49 + MwoI GCNNNNNNNGC 45 42 . . 46 49 + AciI CCGC 46 48 . . 43 54 - CspCI CAANNNNNGTGG 67 65 32 30 73 76 + MaeI CTAG 73 75 . . 87 91 + BceAI ACGGC 103 105 . . 97 102 + NlaIV GGNNCC 99 99 . . 97 102 + HgiCI* GGYRCC 97 101 . . 99 104 + TaqII CACCCA 115 113 . . 102 112 + BsiYI* CCNNNNNNNGG 108 105 . . 102 112 + PflMI CCANNNNNTGG 108 105 . . 113 117 + SfaNI GCATC 122 126 . . 116 120 - HphI GGTGA 108 107 . . 121 124 + DpnI GATC 122 122 . . 121 124 + BstKTI GATC 123 121 . . 121 124 + MboI GATC 120 124 . . 123 128 + Hpy178III* TCNNGA 124 126 . . 121 125 - BinI* GGATC 115 116 . . 135 140 + SalI GTCGAC 135 139 . . 135 140 + Hpy166II GTNNAC 137 137 . . 135 140 + HindII GTYRAC 137 137 . . 135 140 + AccI GTMKAC 136 138 . . 136 139 + TaqI TCGA 136 138 . . 150 153 - MnlI CCTC 143 142 . . 156 161 + Cac8I GCNNGC 158 158 . . 159 162 + CviJI RGCY 160 160 . . 156 159 - AciI CCGC 156 158 . . 156 160 - FauI CCCGC 149 151 . . 165 170 + MmeI TCCRAC 190 188 . . 165 169 + Hpy188I TCNGA 167 166 . . 176 179 + SetI ASST 179 175 . . 176 179 + CviJI RGCY 177 177 . . 176 179 + AluI AGCT 177 177 . . 187 190 + CviJI RGCY 188 188 . . 185 188 - MnlI CCTC 178 177 . . 199 202 + MseI TTAA 199 201 . . 203 206 + CviJI RGCY 204 204 . . 204 209 + Cac8I GCNNGC 206 206 . . 208 212 + BlsI GCNGC 210 209 . . 208 212 + BisI GCNGC 209 210 . . 208 212 + TseI GCWGC 208 211 . . 211 215 + TauI GCSGC 214 211 . . 206 211 - EcoP15I CAGCAG 178 180 . . 211 215 + BlsI GCNGC 213 212 . . 211 215 + BisI GCNGC 212 213 . . 212 217 + NspBII* CMGCKG 214 214 . . 208 212 - BbvI GCAGC 195 199 . . 212 215 + AciI CCGC 212 214 . . 218 222 + Tsp4CI* ACNGT 220 219 . . 230 233 + Hin6I GCGC 230 232 . . 230 233 + GlaI GCGC 231 231 . . 230 233 + HhaI GCGC 232 230 . . 245 250 + DsaI* CCRYGG 245 249 . . 245 250 + SecI* CCNNGG 245 249 . . 247 251 + Tsp4CI* ACNGT 249 248 . . 249 253 + HphI GGTGA 261 260 . . 250 254 + Tsp45I GTSAC 249 254 . . 250 254 + MaeIII GTNAC 249 254 . . 252 256 + HgaI GACGC 261 266 . . 252 257 + AcyI GRCGYC 253 255 . . 255 259 + TauI GCSGC 258 255 . . 255 259 + BlsI GCNGC 257 256 . . 255 259 + BisI GCNGC 256 257 . . 256 259 + AciI CCGC 256 258 . . 258 262 + TauI GCSGC 261 258 . . 258 268 + MwoI GCNNNNNNNGC 264 261 . . 258 262 + BlsI GCNGC 260 259 . . 258 262 + BisI GCNGC 259 260 . . 259 262 + AciI CCGC 259 261 . . 292 295 + MnlI CCTC 302 301 . . 301 304 + TspEI AATT 300 304 . . 328 337 + MslI CAYNNNNRTG 332 332 . . 331 334 + MnlI CCTC 341 340 . . 328 332 - FokI GGATG 314 318 . . 328 332 - BseGI GGATG 327 325 . . 334 337 + FatI CATG 333 337 . . 334 337 + NlaIII CATG 337 333 . . 334 337 + CviAII CATG 334 336 . . 347 352 + DsaI* CCRYGG 347 351 . . 347 352 + StyI CCWWGG 347 351 . . 347 352 + NcoI CCATGG 347 351 . . 347 352 + SecI* CCNNGG 347 351 . . 348 351 + FatI CATG 347 351 . . 348 351 + NlaIII CATG 351 347 . . 348 351 + CviAII CATG 348 350 . . 356 360 + TspDTI ATGAA 371 369 . . 358 362 + MboII GAAGA 370 369 . . 358 363 + BbvII* GAAGAC 365 369 . . 361 365 + HgaI GACGC 370 375 . . 374 377 + FatI CATG 373 377 . . 374 377 + NlaIII CATG 377 373 . . 374 377 + CviAII CATG 374 376 . . 370 374 - HgaI GACGC 359 364 . . 376 379 + CviRI* TGCA 377 377 . . 378 387 + MslI CAYNNNNRTG 382 382 . . 393 396 + TspEI AATT 392 396 . . 398 401 + FnuDII* CGCG 399 399 . . 399 402 + Hin6I GCGC 399 401 . . 399 402 + GlaI GCGC 400 400 . . 399 402 + HhaI GCGC 401 399 . . 401 411 + MwoI GCNNNNNNNGC 407 404 . . 415 418 + TaqI TCGA 415 417 . . 421 424 + SetI ASST 424 420 . . 422 425 + MnlI CCTC 432 431 . . 425 428 + MnlI CCTC 435 434 . . 423 428 - BseRI GAGGAG 414 412 . . 439 444 + SpeI ACTAGT 439 443 . . 440 443 + MaeI CTAG 440 442 . . 447 450 + TspEI AATT 446 450 . . 456 460 - BsmAI GTCTC 450 454 . . 469 472 + CviJI RGCY 470 470 . . 469 472 + HaeIII GGCC 470 470 . . 469 473 + BmgT120I GGNCC 470 471 . . 469 473 + AsuI* GGNCC 469 472 . . 479 483 + Tsp45I GTSAC 478 483 . . 479 483 + MaeIII GTNAC 478 483 . . 481 490 + PshAI GACNNNNGTC 485 485 . . 519 522 + TspEI AATT 518 522 . . 534 539 + Hpy178III* TCNNGA 535 537 . . 558 561 + DpnI GATC 559 559 . . 558 561 + BstKTI GATC 560 558 . . 558 561 + MboI GATC 557 561 . . 560 565 + Hpy178III* TCNNGA 561 563 . . 562 565 + TaqI TCGA 562 564 . . 563 574 + BcgI CGANNNNNNTGC 552 550 586 584 576 579 + Csp6I GTAC 576 578 . . 576 579 + RsaI GTAC 577 577 . . 573 576 - AciI CCGC 573 575 . . 578 581 + TaiI ACGT 581 577 . . 578 581 + SetI ASST 581 577 . . 578 581 + MaeII ACGT 578 580 . . 581 586 + Hpy178III* TCNNGA 582 584 . . 585 589 + HinfI GANTC 585 588 . . 588 593 + Hpy178III* TCNNGA 589 591 . . 585 589 - PleI GAGTC 579 580 . . 585 589 - MlyI GAGTC 579 579 . . 602 605 + CviJI RGCY 603 603 . . 609 612 + TaqI TCGA 609 611 . . 611 614 - MnlI CCTC 604 603 . . 614 619 + Cac8I GCNNGC 616 616 . . 614 625 + AlfI GCANNNNNNTGC 603 601 637 635 614 625 - AlfI GCANNNNNNTGC 637 635 603 601 624 627 - AciI CCGC 624 626 . . 628 633 + SspI AATATT 630 630 . . 639 644 + BetI* WCCGGW 639 643 . . 640 643 + HpaII CCGG 640 642 . . 642 646 + FokI GGATG 655 659 . . 642 646 + BseGI GGATG 648 646 . . 655 658 - MnlI CCTC 648 647 . . 659 662 + Csp6I GTAC 659 661 . . 659 662 + RsaI GTAC 660 660 . . 664 667 + CviJI RGCY 665 665 . . 680 683 + MseI TTAA 680 682 . . 711 714 + SetI ASST 714 710 . . 716 721 + Hpy166II GTNNAC 718 718 . . 722 725 + FatI CATG 721 725 . . 722 725 + NlaIII CATG 725 721 . . 722 725 + CviAII CATG 722 724 . . 723 728 + EcoT22I ATGCAT 727 723 . . 724 727 + CviRI* TGCA 725 725 . . 728 731 + MseI TTAA 728 730 . . 738 743 - BsmI GAATGC 738 736 . . 742 746 - MboII GAAGA 734 733 . . 745 749 - MboII GAAGA 737 736 . . 748 752 - MboII GAAGA 740 739 . . 757 760 + TspEI AATT 756 760 . . 778 783 + TatI WGTACW 778 782 . . 779 782 + Csp6I GTAC 779 781 . . 779 782 + RsaI GTAC 780 780 . . 793 796 + CviJI RGCY 794 794 . . 804 807 + SetI ASST 807 803 . . 804 807 + CviJI RGCY 805 805 . . 804 807 + AluI AGCT 805 805 . . 819 823 - TspDTI ATGAA 809 807 . . 821 825 - FokI GGATG 807 811 . . 821 825 - BseGI GGATG 820 818 . . 835 838 + MseI TTAA 835 837 . . 838 843 + ApoI RAATTY 838 842 . . 839 842 + TspEI AATT 838 842 . . 842 845 + MseI TTAA 842 844 . . 868 871 + SetI ASST 871 867 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 136 764 # # AciI: # [CCGC] # 3 44 46 51 56 110 # 276 314 # # AcyI: # [GRCGYC] # 253 647 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 263 603 # # AluI: # [AGCT] # 95 177 628 # # ApoI: # [RAATTY] # 62 838 # # AsuI*: # [GGNCC] # 431 469 # # BbvI: # [GCAGC] # 195 705 # # BbvII*: # [GAAGAC] # 365 535 # # BceAI: # [ACGGC] # 103 797 # # BcgI: # [CGANNNNNNTGC] # 34 314 552 # # BetI*: # [WCCGGW] # 261 639 # # BinI*: # [GGATC] # 115 785 # # BisI: # [GCNGC] # 3 3 44 209 641 # # BlsI: # [GCNGC] # 3 3 44 210 640 # # BmgT120I: # [GGNCC] # 430 470 # # BseGI: # [GGATG] # 80 172 321 327 # # BseRI: # [GAGGAG] # 414 486 # # BsiYI*: # [CCNNNNNNNGG] # 108 792 # # BsmAI: # [GTCTC] # 450 450 # # BsmI: # [GAATGC] # 162 738 # # BstKTI: # [GATC] # 4 33 86 340 437 # # Cac8I: # [GCNNGC] # 48 158 284 410 # # ClaI: # [ATCGAT] # 33 867 # # Csp6I: # [GTAC] # 83 120 121 576 # # CspCI: # [CAANNNNNGTGG] # 32 35 833 # # CviAII: # [CATG] # 14 26 178 334 348 # # CviJI: # [RGCY] # 11 11 16 17 62 95 # 129 133 160 266 # # CviRI*: # [TGCA] # 175 348 377 # # DpnI: # [GATC] # 4 32 86 341 437 # # DsaI*: # [CCRYGG] # 102 245 553 # # EcoP15I: # [CAGCAG] # 178 722 # # EcoT22I: # [ATGCAT] # 173 727 # # FatI: # [CATG] # 14 26 179 333 348 # # FauI: # [CCCGC] # 149 751 # # FnuDII*: # [CGCG] # 399 501 # # FokI: # [GGATG] # 93 152 314 341 # # GlaI: # [GCGC] # 169 231 500 # # HaeIII: # [GGCC] # 430 470 # # HgaI: # [GACGC] # 11 98 261 530 # # HgiCI*: # [GGYRCC] # 97 803 # # HhaI: # [GCGC] # 169 232 499 # # Hin6I: # [GCGC] # 169 230 501 # # HindII: # [GTYRAC] # 137 763 # # HinfI: # [GANTC] # 315 585 # # HpaII: # [CCGG] # 260 640 # # HphI: # [GGTGA] # 108 153 639 # # Hpy166II: # [GTNNAC] # 5 132 182 581 # # Hpy178III*: # [TCNNGA] # 7 21 26 124 311 411 # # Hpy188I: # [TCNGA] # 167 733 # # MaeI: # [CTAG] # 73 367 460 # # MaeII: # [ACGT] # 322 578 # # MaeIII: # [GTNAC] # 229 249 422 # # MboI: # [GATC] # 4 30 86 343 437 # # MboII: # [GAAGA] # 3 3 160 364 370 # # McrI*: # [CGRYCG] # 37 863 # # MlyI: # [GAGTC] # 321 579 # # MmeI: # [TCCRAC] # 190 710 # # MnlI: # [CCTC] # 3 35 39 44 91 124 # 143 169 252 # # MseI: # [TTAA] # 7 48 58 107 199 481 # # MslI: # [CAYNNNNRTG] # 50 332 518 # # MwoI: # [GCNNNNNNNGC] # 45 143 219 493 # # NcoI: # [CCATGG] # 347 553 # # NlaIII: # [CATG] # 14 26 175 337 348 # # NlaIV: # [GGNNCC] # 99 801 # # NspBII*: # [CMGCKG] # 214 686 # # PflMI: # [CCANNNNNTGG] # 108 792 # # PleI: # [GAGTC] # 321 579 # # PshAI: # [GACNNNNGTC] # 415 485 # # PvuI: # [CGATCG] # 37 863 # # RsaI: # [GTAC] # 83 120 120 577 # # SalI: # [GTCGAC] # 135 765 # # SecI*: # [CCNNGG] # 102 245 553 # # SetI: # [ASST] # 29 64 93 133 157 179 # 245 # # SfaNI: # [GCATC] # 122 778 # # SpeI: # [ACTAGT] # 439 461 # # SspI: # [AATATT] # 270 630 # # StyI: # [CCWWGG] # 347 553 # # TaiI: # [ACGT] # 319 581 # # TaqI: # [TCGA] # 33 47 103 147 279 291 # # TaqII: # [CACCCA] # 115 785 # # TatI: # [WGTACW] # 122 778 # # TauI: # [GCSGC] # 3 44 214 639 # # TseI: # [GCWGC] # 208 692 # # Tsp45I: # [GTSAC] # 229 249 422 # # Tsp4CI*: # [ACNGT] # 18 29 202 651 # # TspDTI: # [ATGAA] # 91 371 438 # # TspEI: # [AATT] # 54 62 72 82 92 238 # 300 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 900 # Reported_sequences: 1 # Reported_hitcount: 192 #---------------------------------------