Restriction Fragments for RPS2/YGL123W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

RPS2/YGL123W on chromosome VII from coordinates 277617 to 278381.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27175.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 7 17 + MwoI GCNNNNNNNGC 13 10 . . 9 14 + Hpy178III* TCNNGA 10 12 . . 15 18 + SetI ASST 18 14 . . 15 18 + CviJI RGCY 16 16 . . 15 18 + AluI AGCT 16 16 . . 33 36 + SetI ASST 36 32 . . 32 35 - MnlI CCTC 25 24 . . 45 50 + CfrI YGGCCR 45 49 . . 46 49 + CviJI RGCY 47 47 . . 46 49 + HaeIII GGCC 47 47 . . 50 54 + MaeIII GTNAC 49 54 . . 47 51 - BceAI ACGGC 32 34 . . 58 61 + CviJI RGCY 59 59 . . 58 61 + HaeIII GGCC 59 59 . . 56 59 - MnlI CCTC 49 48 . . 59 63 - BceAI ACGGC 44 46 . . 71 75 + MboII GAAGA 83 82 . . 71 76 - Ksp632I* CTCTTC 66 69 . . 76 80 + BmgT120I GGNCC 77 78 . . 76 80 + AvaII GGWCC 76 79 . . 76 80 + AsuI* GGNCC 76 79 . . 74 77 - MnlI CCTC 67 66 . . 87 93 + TspRI CASTGNN 93 84 . . 89 94 + Eco57MI CTGRAG 110 108 . . 89 94 + Eco57I CTGAAG 110 108 . . 91 95 + MboII GAAGA 103 102 . . 100 104 + FokI GGATG 113 117 . . 100 104 + BseGI GGATG 106 104 . . 105 110 + NlaIV GGNNCC 107 107 . . 101 105 - BccI CCATC 95 96 . . 112 116 + MaeIII GTNAC 111 116 . . 109 113 - BsrI ACTGG 109 107 . . 121 124 + MaeI CTAG 121 123 . . 123 126 + SetI ASST 126 122 . . 138 141 + CviJI RGCY 139 139 . . 160 164 + MboII GAAGA 172 171 . . 167 171 - MboII GAAGA 159 158 . . 173 176 + CviRI* TGCA 174 174 . . 174 184 + MwoI GCNNNNNNNGC 180 177 . . 174 184 + BstAPI GCANNNNNTGC 180 177 . . 184 188 - BsrI ACTGG 184 182 . . 193 198 + EcoRI GAATTC 193 197 . . 193 198 + ApoI RAATTY 193 197 . . 194 197 + TspEI AATT 193 197 . . 219 229 + MwoI GCNNNNNNNGC 225 222 . . 220 224 + BssKI CCNGG 219 224 . . 220 224 + BseBI CCWGG 221 222 . . 220 224 + ScrFI CCNGG 221 222 . . 220 224 + EcoRII CCWGG 219 224 . . 222 225 + SetI ASST 225 221 . . 227 230 + CviRI* TGCA 228 228 . . 232 240 + Tth111I GACNNNGTC 235 236 . . 239 244 + Hpy178III* TCNNGA 240 242 . . 239 244 + BspHI TCATGA 239 243 . . 240 243 + FatI CATG 239 243 . . 240 243 + NlaIII CATG 243 239 . . 240 243 + CviAII CATG 240 242 . . 241 245 + TspDTI ATGAA 256 254 . . 251 254 + CviJI RGCY 252 252 . . 253 257 - BsrI ACTGG 253 251 . . 273 276 + CviJI RGCY 274 274 . . 274 279 + Cfr10I RCCGGY 274 278 . . 275 278 + HpaII CCGG 275 277 . . 293 296 + MseI TTAA 293 295 . . 297 300 + CviJI RGCY 298 298 . . 313 317 + HphI GGTGA 325 324 . . 314 318 + Tsp45I GTSAC 313 318 . . 314 318 + MaeIII GTNAC 313 318 . . 316 320 + HinfI GANTC 316 319 . . 316 320 - PleI GAGTC 310 311 . . 316 320 - MlyI GAGTC 310 310 . . 323 327 + Tsp4CI* ACNGT 325 324 . . 326 330 + Tsp45I GTSAC 325 330 . . 326 330 + MaeIII GTNAC 325 330 . . 329 332 + TaiI ACGT 332 328 . . 329 332 + SetI ASST 332 328 . . 329 332 + MaeII ACGT 329 331 . . 344 349 + Hpy178III* TCNNGA 345 347 . . 350 353 + AciI CCGC 350 352 . . 353 358 + StyI CCWWGG 353 357 . . 353 358 + SecI* CCNNGG 353 357 . . 367 372 + NlaIV GGNNCC 369 369 . . 367 372 + HgiCI* GGYRCC 367 371 . . 370 380 + MwoI GCNNNNNNNGC 376 373 . . 371 375 + BccI CCATC 379 380 . . 374 378 + Hpy188I TCNGA 376 375 . . 378 381 + SetI ASST 381 377 . . 378 381 + CviJI RGCY 379 379 . . 378 381 + AluI AGCT 379 379 . . 377 387 - Hin4I GAYNNNNNVTC 400 395 368 363 403 407 - TspGWI ACGGA 393 391 . . 416 420 + Hpy188I TCNGA 418 417 . . 419 423 + MboII GAAGA 431 430 . . 423 426 + SetI ASST 426 422 . . 419 424 - Ksp632I* CTCTTC 414 417 . . 422 425 - MnlI CCTC 415 414 . . 425 429 + MaeIII GTNAC 424 429 . . 428 432 + BsrI ACTGG 433 431 . . 428 433 + BfiI ACTGGG 438 437 . . 428 438 + BaeI ACNNNNGTAYC 417 412 450 445 433 438 + KpnI GGTACC 437 433 . . 433 438 + NlaIV GGNNCC 435 435 . . 433 438 + HgiCI* GGYRCC 433 437 . . 433 438 + Acc65I GGTACC 433 437 . . 434 437 + Csp6I GTAC 434 436 . . 434 437 + RsaI GTAC 435 435 . . 446 451 + Hpy166II GTNNAC 448 448 . . 446 451 + HindII GTYRAC 448 448 . . 461 466 + BalI TGGCCA 463 463 . . 461 466 + CfrI YGGCCR 461 465 . . 462 465 + CviJI RGCY 463 463 . . 451 462 - CspCI CAANNNNNGTGG 475 473 440 438 462 465 + HaeIII GGCC 463 463 . . 474 480 + TspRI CASTGNN 480 471 . . 475 479 + BsrI ACTGG 480 478 . . 487 492 + NlaIV GGNNCC 489 489 . . 479 491 - PsrI GAACNNNNNNTAC 503 498 471 466 493 497 + Tsp45I GTSAC 492 497 . . 493 497 + MaeIII GTNAC 492 497 . . 490 494 - TspGWI ACGGA 480 478 . . 495 501 + TspRI CASTGNN 501 492 . . 496 500 + Tsp4CI* ACNGT 498 497 . . 507 510 + DpnI GATC 508 508 . . 507 510 + BstKTI GATC 509 507 . . 507 510 + MboI GATC 506 510 . . 510 515 + GsaI CCCAGC 514 510 . . 510 515 + BseYI CCCAGC 510 514 . . 507 511 - BinI* GGATC 501 502 . . 513 516 + CviJI RGCY 514 514 . . 522 525 + SetI ASST 525 521 . . 521 524 - MnlI CCTC 514 513 . . 534 538 + Hpy99I CGWCG 538 533 . . 538 548 + MwoI GCNNNNNNNGC 544 541 . . 530 542 - BarI GAAGNNNNNNTAC 554 549 522 517 545 550 + PvuII CAGCTG 547 547 . . 545 550 + NspBII* CMGCKG 547 547 . . 546 549 + SetI ASST 549 545 . . 546 549 + CviJI RGCY 547 547 . . 546 549 + AluI AGCT 547 547 . . 542 547 - Eco57MI CTGRAG 527 525 . . 542 547 - GsuI CTGGAG 527 525 . . 563 566 + CviRI* TGCA 564 564 . . 565 570 + MfeI CAATTG 565 569 . . 566 569 + TspEI AATT 565 569 . . 570 573 + CviJI RGCY 571 571 . . 580 584 + MboII GAAGA 592 591 . . 586 591 + Hpy166II GTNNAC 588 588 . . 586 591 + AccI GTMKAC 587 589 . . 591 596 + TaqII CACCCA 607 605 . . 602 606 + Tsp4CI* ACNGT 604 603 . . 611 614 + MaeI CTAG 611 613 . . 628 631 + SetI ASST 631 627 . . 636 639 + CviJI RGCY 637 637 . . 633 637 - Hin4II* CCTTC 627 626 . . 637 641 + BlsI GCNGC 639 638 . . 637 641 + BisI GCNGC 638 639 . . 637 641 + TseI GCWGC 637 640 . . 640 650 + MwoI GCNNNNNNNGC 646 643 . . 637 641 - BbvI GCAGC 624 628 . . 656 660 + MaeIII GTNAC 655 660 . . 665 669 + Tsp4CI* ACNGT 667 666 . . 671 676 + Hpy178III* TCNNGA 672 674 . . 675 679 + HinfI GANTC 675 678 . . 675 679 - PleI GAGTC 669 670 . . 675 679 - MlyI GAGTC 669 669 . . 679 690 + BstXI CCANNNNNNTGG 686 682 . . 689 693 + BmgT120I GGNCC 690 691 . . 689 693 + AsuI* GGNCC 689 692 . . 690 693 + CviJI RGCY 691 691 . . 690 693 + HaeIII GGCC 691 691 . . 701 706 - BsrDI GCAATG 700 698 . . 706 710 - BsrI ACTGG 706 704 . . 721 731 + Hin4I GAYNNNNNVTC 712 707 744 739 722 732 + BsaXI ACNNNNNCTCC 712 709 742 739 730 734 + Hpy188I TCNGA 732 731 . . 734 738 + TspDTI ATGAA 749 747 . . 737 742 + HindIII AAGCTT 737 741 . . 738 741 + SetI ASST 741 737 . . 738 741 + CviJI RGCY 739 739 . . 738 741 + AluI AGCT 739 739 . . 753 762 + XmnI GAANNNNTTC 757 757 . . 758 762 + TfiI GAWTC 758 761 . . 758 762 + HinfI GANTC 758 761 . . 753 758 - Ksp632I* CTCTTC 748 751 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 332 433 # # AccI: # [GTMKAC] # 178 587 # # AciI: # [CCGC] # 350 415 # # AluI: # [AGCT] # 16 26 168 192 363 # # ApoI: # [RAATTY] # 193 572 # # AsuI*: # [GGNCC] # 76 76 613 # # AvaII: # [GGWCC] # 76 689 # # BaeI: # [ACNNNNGTAYC] # 33 315 417 # # BalI: # [TGGCCA] # 302 463 # # BarI: # [GAAGNNNNNNTAC] # 32 211 522 # # BbvI: # [GCAGC] # 141 624 # # BccI: # [CCATC] # 95 284 386 # # BceAI: # [ACGGC] # 12 32 721 # # BfiI: # [ACTGGG] # 327 438 # # BinI*: # [GGATC] # 264 501 # # BisI: # [GCNGC] # 127 638 # # BlsI: # [GCNGC] # 126 639 # # BmgT120I: # [GGNCC] # 75 77 613 # # BsaXI: # [ACNNNNNCTCC] # 23 30 712 # # BseBI: # [CCWGG] # 221 544 # # BseGI: # [GGATG] # 106 659 # # BseYI: # [CCCAGC] # 255 510 # # BspHI: # [TCATGA] # 239 526 # # BsrDI: # [GCAATG] # 65 700 # # BsrI: # [ACTGG] # 47 59 69 75 109 180 # 226 # # BssKI: # [CCNGG] # 219 546 # # BstAPI: # [GCANNNNNTGC] # 180 585 # # BstKTI: # [GATC] # 256 509 # # BstXI: # [CCANNNNNNTGG] # 79 686 # # Cfr10I: # [RCCGGY] # 274 491 # # CfrI: # [YGGCCR] # 45 304 416 # # Csp6I: # [GTAC] # 331 434 # # CspCI: # [CAANNNNNGTGG] # 35 290 440 # # CviAII: # [CATG] # 240 525 # # CviJI: # [RGCY] # 12 16 22 24 24 26 # 31 33 48 51 54 66 # 80 81 84 113 # # CviRI*: # [TGCA] # 54 174 201 336 # # DpnI: # [GATC] # 257 508 # # Eco57I: # [CTGAAG] # 110 655 # # Eco57MI: # [CTGRAG] # 110 238 417 # # EcoRI: # [GAATTC] # 193 572 # # EcoRII: # [CCWGG] # 219 546 # # FatI: # [CATG] # 239 526 # # FokI: # [GGATG] # 113 652 # # GsaI: # [CCCAGC] # 251 514 # # GsuI: # [CTGGAG] # 238 527 # # HaeIII: # [GGCC] # 12 47 74 228 404 # # HgiCI*: # [GGYRCC] # 66 332 367 # # Hin4I: # [GAYNNNNNVTC] # 21 32 32 312 368 # # Hin4II*: # [CCTTC] # 138 627 # # HindII: # [GTYRAC] # 317 448 # # HindIII: # [AAGCTT] # 28 737 # # HinfI: # [GANTC] # 7 83 316 359 # # HpaII: # [CCGG] # 275 490 # # HphI: # [GGTGA] # 325 440 # # Hpy166II: # [GTNNAC] # 140 177 448 # # Hpy178III*: # [TCNNGA] # 10 93 105 230 327 # # Hpy188I: # [TCNGA] # 33 42 314 376 # # Hpy99I: # [CGWCG] # 227 538 # # KpnI: # [GGTACC] # 328 437 # # Ksp632I*: # [CTCTTC] # 17 66 334 348 # # MaeI: # [CTAG] # 121 154 490 # # MaeII: # [ACGT] # 329 436 # # MaeIII: # [GTNAC] # 12 49 62 68 99 110 # 163 202 # # MboI: # [GATC] # 259 506 # # MboII: # [GAAGA] # 13 20 56 83 161 173 # 259 # # MfeI: # [CAATTG] # 200 565 # # MlyI: # [GAGTC] # 96 310 359 # # MnlI: # [CCTC] # 18 24 25 99 251 348 # # MseI: # [TTAA] # 293 472 # # MwoI: # [GCNNNNNNNGC] # 13 45 102 119 151 167 # 168 # # NlaIII: # [CATG] # 243 522 # # NlaIV: # [GGNNCC] # 54 66 107 262 276 # # NspBII*: # [CMGCKG] # 218 547 # # PleI: # [GAGTC] # 96 310 359 # # PsrI: # [GAACNNNNNNTAC] # 32 262 471 # # PvuII: # [CAGCTG] # 218 547 # # RsaI: # [GTAC] # 330 435 # # ScrFI: # [CCNGG] # 221 544 # # SecI*: # [CCNNGG] # 353 412 # # SetI: # [ASST] # 18 18 24 24 45 49 # 82 90 99 99 107 110 # # StyI: # [CCWWGG] # 353 412 # # TaiI: # [ACGT] # 332 433 # # TaqII: # [CACCCA] # 158 607 # # TfiI: # [GAWTC] # 7 758 # # TseI: # [GCWGC] # 128 637 # # Tsp45I: # [GTSAC] # 12 167 273 313 # # Tsp4CI*: # [ACNGT] # 63 98 106 173 325 # # TspDTI: # [ATGAA] # 16 256 493 # # TspEI: # [AATT] # 193 200 372 # # TspGWI: # [ACGGA] # 87 285 393 # # TspRI: # [CASTGNN] # 21 93 264 387 # # Tth111I: # [GACNNNGTC] # 235 530 # # XmnI: # [GAANNNNTTC] # 8 757 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 765 # Reported_sequences: 1 # Reported_hitcount: 184 #---------------------------------------