Restriction Fragments for GPG1/YGL121C



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The currently selected gene/sequence is :

GPG1/YGL121C on chromosome VII from coordinates 281157 to 280777.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.8025.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 10 14 + DdeI CTNAG 10 13 . . 14 18 + Tsp45I GTSAC 13 18 . . 14 18 + MaeIII GTNAC 13 18 . . 20 23 + TaqI TCGA 20 22 . . 22 25 - MnlI CCTC 15 14 . . 25 29 + MboII GAAGA 37 36 . . 30 33 + CviJI RGCY 31 31 . . 32 35 + MnlI CCTC 42 41 . . 32 37 + SecI* CCNNGG 32 36 . . 32 42 + BsiYI* CCNNNNNNNGG 38 35 . . 36 39 + CviJI RGCY 37 37 . . 33 38 - NmeAIII GCCGAG 13 11 . . 37 42 - GsaI CCCAGC 41 37 . . 42 45 + CviJI RGCY 43 43 . . 37 42 - BseYI CCCAGC 37 41 . . 54 59 + PsiI TTATAA 56 56 . . 58 61 + TspEI AATT 57 61 . . 89 94 + SmlI CTYRAG 89 93 . . 90 95 + Hpy178III* TCNNGA 91 93 . . 89 94 - Bce83I* CTTGAG 74 72 . . 98 101 + SetI ASST 101 97 . . 99 102 + MnlI CCTC 109 108 . . 103 107 + Tsp45I GTSAC 102 107 . . 103 107 + MaeIII GTNAC 102 107 . . 106 110 + Tsp4CI* ACNGT 108 107 . . 104 108 - HphI GGTGA 96 95 . . 113 117 + Hpy188I TCNGA 115 114 . . 112 117 - MmeI TCCRAC 93 91 . . 122 125 + CviRI* TGCA 123 123 . . 135 140 + McrI* CGRYCG 138 136 . . 135 140 + PvuI CGATCG 138 136 . . 136 139 + DpnI GATC 137 137 . . 136 139 + BstKTI GATC 138 136 . . 136 139 + MboI GATC 135 139 . . 146 150 - TspDTI ATGAA 136 134 . . 156 159 + TspEI AATT 155 159 . . 161 164 + CviJI RGCY 162 162 . . 166 170 + TfiI GAWTC 166 169 . . 166 170 + HinfI GANTC 166 169 . . 160 170 - Hin4I GAYNNNNNVTC 183 178 151 146 178 182 + MaeIII GTNAC 177 182 . . 191 197 + TspRI CASTGNN 197 188 . . 191 196 - BtsI GCAGTG 190 188 . . 196 200 + BccI CCATC 204 205 . . 217 220 + MaeI CTAG 217 219 . . 227 232 + NlaIV GGNNCC 229 229 . . 231 235 + BssKI CCNGG 230 235 . . 231 235 + BseBI CCWGG 232 233 . . 231 235 + ScrFI CCNGG 232 233 . . 231 235 + EcoRII CCWGG 230 235 . . 240 243 + TaiI ACGT 243 239 . . 240 243 + SetI ASST 243 239 . . 240 243 + MaeII ACGT 240 242 . . 237 241 - BsmAI GTCTC 231 235 . . 237 242 - Esp3I CGTCTC 231 235 . . 252 256 + FokI GGATG 265 269 . . 252 256 + BseGI GGATG 258 256 . . 265 269 + TspDTI ATGAA 280 278 . . 274 278 + Hpy188I TCNGA 276 275 . . 279 283 + BsrI ACTGG 284 282 . . 284 288 + BsrI ACTGG 289 287 . . 284 289 + BfiI ACTGGG 294 293 . . 287 291 + BslFI GGGAC 301 305 . . 293 296 + TaqI TCGA 293 295 . . 294 298 + Hpy99I CGWCG 298 293 . . 303 308 + Hpy166II GTNNAC 305 305 . . 309 313 + Tsp4CI* ACNGT 311 310 . . 312 316 + MaeIII GTNAC 311 316 . . 319 323 + MboII GAAGA 331 330 . . 323 326 + SetI ASST 326 322 . . 323 326 + CviJI RGCY 324 324 . . 323 326 + AluI AGCT 324 324 . . 319 324 - Ksp632I* CTCTTC 314 317 . . 319 325 - SapI GCTCTTC 314 317 . . 354 359 + BciVI GTATCC 365 364 . . 358 363 + StyI CCWWGG 358 362 . . 358 363 + SecI* CCNNGG 358 362 . . 358 363 + AvrII CCTAGG 358 362 . . 359 362 + MaeI CTAG 359 361 . . 367 370 + SetI ASST 370 366 . . 365 369 - Hin4II* CCTTC 359 358 . . 378 381 + MaeI CTAG 378 380 . . #--------------------------------------- # # # AluI: # [AGCT] # 57 324 # # AvrII: # [CCTAGG] # 23 358 # # BccI: # [CCATC] # 177 204 # # Bce83I*: # [CTTGAG] # 74 307 # # BciVI: # [GTATCC] # 16 365 # # BfiI: # [ACTGGG] # 87 294 # # BseBI: # [CCWGG] # 149 232 # # BseGI: # [GGATG] # 123 258 # # BseYI: # [CCCAGC] # 37 344 # # BsiYI*: # [CCNNNNNNNGG] # 38 343 # # BslFI: # [GGGAC] # 80 301 # # BsmAI: # [GTCTC] # 150 231 # # BsrI: # [ACTGG] # 5 92 284 # # BssKI: # [CCNGG] # 151 230 # # BstKTI: # [GATC] # 138 243 # # BtsI: # [GCAGTG] # 190 191 # # CviJI: # [RGCY] # 6 6 31 57 119 162 # # CviRI*: # [TGCA] # 123 258 # # DdeI: # [CTNAG] # 10 371 # # DpnI: # [GATC] # 137 244 # # EcoRII: # [CCWGG] # 151 230 # # Esp3I: # [CGTCTC] # 150 231 # # FokI: # [GGATG] # 116 265 # # GsaI: # [CCCAGC] # 41 340 # # Hin4I: # [GAYNNNNNVTC] # 32 151 198 # # Hin4II*: # [CCTTC] # 22 359 # # HinfI: # [GANTC] # 166 215 # # HphI: # [GGTGA] # 96 285 # # Hpy166II: # [GTNNAC] # 76 305 # # Hpy178III*: # [TCNNGA] # 91 290 # # Hpy188I: # [TCNGA] # 105 115 161 # # Hpy99I: # [CGWCG] # 83 298 # # Ksp632I*: # [CTCTTC] # 67 314 # # MaeI: # [CTAG] # 3 19 142 217 # # MaeII: # [ACGT] # 141 240 # # MaeIII: # [GTNAC] # 13 70 75 89 134 # # MboI: # [GATC] # 135 246 # # MboII: # [GAAGA] # 37 50 294 # # McrI*: # [CGRYCG] # 138 243 # # MmeI: # [TCCRAC] # 93 288 # # MnlI: # [CCTC] # 15 27 67 272 # # NlaIV: # [GGNNCC] # 152 229 # # NmeAIII: # [GCCGAG] # 13 368 # # PsiI: # [TTATAA] # 56 325 # # PvuI: # [CGATCG] # 138 243 # # SapI: # [GCTCTTC] # 67 314 # # ScrFI: # [CCNGG] # 149 232 # # SecI*: # [CCNNGG] # 23 32 326 # # SetI: # [ASST] # 11 44 83 101 142 # # SmlI: # [CTYRAG] # 89 292 # # StyI: # [CCWWGG] # 23 358 # # TaiI: # [ACGT] # 138 243 # # TaqI: # [TCGA] # 20 88 273 # # TfiI: # [GAWTC] # 166 215 # # Tsp45I: # [GTSAC] # 13 89 279 # # Tsp4CI*: # [ACNGT] # 70 108 203 # # TspDTI: # [ATGAA] # 101 136 144 # # TspEI: # [AATT] # 57 98 226 # # TspRI: # [CASTGNN] # 184 197 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 381 # Reported_sequences: 1 # Reported_hitcount: 82 #---------------------------------------