Restriction Fragments for SEH1/YGL100W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SEH1/YGL100W on chromosome VII from coordinates 313234 to 314283.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25724.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 22 25 + FatI CATG 21 25 . . 22 25 + NlaIII CATG 25 21 . . 22 25 + CviAII CATG 22 24 . . 27 38 + BdaI TGANNNNNNTCA 16 14 50 48 36 41 + Hpy178III* TCNNGA 37 39 . . 36 41 + BspHI TCATGA 36 40 . . 37 40 + FatI CATG 36 40 . . 37 40 + NlaIII CATG 40 36 . . 37 40 + CviAII CATG 37 39 . . 27 38 - BdaI TGANNNNNNTCA 50 48 16 14 35 39 - TspDTI ATGAA 25 23 . . 59 63 + TspGWI ACGGA 74 72 . . 62 66 + MboII GAAGA 74 73 . . 62 67 + BbvII* GAAGAC 69 73 . . 66 71 + BspLU11I* ACATGT 66 70 . . 66 71 + NspI RCATGY 70 66 . . 66 71 + AflIII ACRYGT 66 70 . . 67 70 + FatI CATG 66 70 . . 67 70 + NlaIII CATG 70 66 . . 67 70 + CviAII CATG 67 69 . . 61 72 - TstI CACNNNNNNTCC 85 80 53 48 76 81 + NspI RCATGY 80 76 . . 77 80 + FatI CATG 76 80 . . 77 80 + NlaIII CATG 80 76 . . 77 80 + CviAII CATG 77 79 . . 80 86 + SapI GCTCTTC 87 90 . . 81 86 + Ksp632I* CTCTTC 87 90 . . 85 89 + Hpy188I TCNGA 87 86 . . 87 92 + BclI TGATCA 87 91 . . 88 91 + DpnI GATC 89 89 . . 88 91 + BstKTI GATC 90 88 . . 88 91 + MboI GATC 87 91 . . 98 101 + MseI TTAA 98 100 . . 106 111 + AhaIII* TTTAAA 108 108 . . 107 110 + MseI TTAA 107 109 . . 110 113 + TspEI AATT 109 113 . . 123 128 + BsiI* CACGAG 123 127 . . 130 133 + TspEI AATT 129 133 . . 139 143 + DdeI CTNAG 139 142 . . 139 143 + BspCNI CTCAG 152 150 . . 139 143 + BseMII CTCAG 153 151 . . 141 147 + TspRI CASTGNN 147 138 . . 143 147 + Tsp45I GTSAC 142 147 . . 143 147 + MaeIII GTNAC 142 147 . . 145 149 + HinfI GANTC 145 148 . . 145 149 - PleI GAGTC 139 140 . . 145 149 - MlyI GAGTC 139 139 . . 149 152 + FatI CATG 148 152 . . 149 152 + NlaIII CATG 152 148 . . 149 152 + CviAII CATG 149 151 . . 155 160 + SacI GAGCTC 159 155 . . 155 160 + Ecl136II GAGCTC 157 157 . . 155 160 + SduI GDGCHC 159 155 . . 155 160 + HgiJII* GRGCYC 159 155 . . 155 160 + HgiAI* GWGCWC 159 155 . . 156 159 + SetI ASST 159 155 . . 156 159 + CviJI RGCY 157 157 . . 156 159 + AluI AGCT 157 157 . . 159 164 + Hpy178III* TCNNGA 160 162 . . 164 168 + Tsp4CI* ACNGT 166 165 . . 164 174 + BaeI ACNNNNGTAYC 153 148 186 181 176 181 + BalI TGGCCA 178 178 . . 176 181 + CfrI YGGCCR 176 180 . . 177 180 + CviJI RGCY 178 178 . . 177 180 + HaeIII GGCC 178 178 . . 179 189 + BsiYI* CCNNNNNNNGG 185 182 . . 179 189 + PflMI CCANNNNNTGG 185 182 . . 188 192 + BmgT120I GGNCC 189 190 . . 188 192 + AsuI* GGNCC 188 191 . . 189 192 + CviJI RGCY 190 190 . . 189 192 + HaeIII GGCC 190 190 . . 195 200 + Hpy178III* TCNNGA 196 198 . . 191 195 - BsrI ACTGG 191 189 . . 196 206 + BsiYI* CCNNNNNNNGG 202 199 . . 196 206 + PflMI CCANNNNNTGG 202 199 . . 207 210 + Hin6I GCGC 207 209 . . 207 210 + GlaI GCGC 208 208 . . 207 210 + HhaI GCGC 209 207 . . 209 213 + SfaNI GCATC 218 222 . . 213 218 - BsrDI GCAATG 212 210 . . 222 225 + CviJI RGCY 223 223 . . 218 223 - Eco57MI CTGRAG 203 201 . . 218 223 - Eco57I CTGAAG 203 201 . . 224 227 + MnlI CCTC 234 233 . . 238 242 + Tsp4CI* ACNGT 240 239 . . 245 248 + TspEI AATT 244 248 . . 253 257 + MboII GAAGA 265 264 . . 256 260 + MboII GAAGA 268 267 . . 258 263 + XhoII RGATCY 258 262 . . 259 262 + DpnI GATC 260 260 . . 259 262 + BstKTI GATC 261 259 . . 259 262 + MboI GATC 258 262 . . 261 266 + Hpy178III* TCNNGA 262 264 . . 259 263 - BinI* GGATC 253 254 . . 271 275 + MboII GAAGA 283 282 . . 274 279 + BsmI GAATGC 280 278 . . 282 287 + CfrI YGGCCR 282 286 . . 283 286 + CviJI RGCY 284 284 . . 283 286 + HaeIII GGCC 284 284 . . 286 290 + Hpy99I CGWCG 290 285 . . 284 288 - BceAI ACGGC 269 271 . . 290 295 - MmeI TCCRAC 271 269 . . 299 302 + SetI ASST 302 298 . . 299 302 + CviJI RGCY 300 300 . . 299 302 + AluI AGCT 300 300 . . 303 308 + Hpy166II GTNNAC 305 305 . . 305 308 + Csp6I GTAC 305 307 . . 305 308 + RsaI GTAC 306 306 . . 316 320 + TfiI GAWTC 316 319 . . 316 320 + HinfI GANTC 316 319 . . 323 333 + FalI AAGNNNNNCTT 314 309 346 341 325 329 + BinI* GGATC 333 334 . . 326 329 + DpnI GATC 327 327 . . 326 329 + BstKTI GATC 328 326 . . 326 329 + MboI GATC 325 329 . . 323 333 - FalI AAGNNNNNCTT 346 341 314 309 343 348 + ApoI RAATTY 343 347 . . 344 347 + TspEI AATT 343 347 . . 349 352 + Hin6I GCGC 349 351 . . 349 352 + GlaI GCGC 350 350 . . 349 352 + HhaI GCGC 351 349 . . 351 356 + Cac8I GCNNGC 353 353 . . 355 358 + Hin6I GCGC 355 357 . . 355 358 + GlaI GCGC 356 356 . . 355 358 + HhaI GCGC 357 355 . . 364 367 + CviJI RGCY 365 365 . . 367 370 + MseI TTAA 367 369 . . 371 374 + TspEI AATT 370 374 . . 375 378 + SetI ASST 378 374 . . 375 378 + CviJI RGCY 376 376 . . 375 378 + AluI AGCT 376 376 . . 386 390 + MaeIII GTNAC 385 390 . . 395 399 + TfiI GAWTC 395 398 . . 395 399 + HinfI GANTC 395 398 . . 391 395 - BccI CCATC 385 386 . . 399 402 + MnlI CCTC 409 408 . . 400 404 + DdeI CTNAG 400 403 . . 400 404 + BspCNI CTCAG 413 411 . . 400 404 + BseMII CTCAG 414 412 . . 401 405 + Hpy188I TCNGA 403 402 . . 412 416 - SfaNI GCATC 402 406 . . 417 421 + DdeI CTNAG 417 420 . . 423 426 + SetI ASST 426 422 . . 424 428 + Hin4II* CCTTC 434 433 . . 427 431 + Hpy188I TCNGA 429 428 . . 430 440 + Hin4I GAYNNNNNVTC 421 416 453 448 430 440 + Eam1105I GACNNNNNGTC 435 434 . . 431 434 + SetI ASST 434 430 . . 432 438 + SauI* CCTNAGG 433 436 . . 433 437 + DdeI CTNAG 433 436 . . 436 439 + SetI ASST 439 435 . . 430 440 - Hin4I GAYNNNNNVTC 453 448 421 416 440 443 + FatI CATG 439 443 . . 440 443 + NlaIII CATG 443 439 . . 440 443 + CviAII CATG 440 442 . . 446 452 + TspRI CASTGNN 452 443 . . 454 458 + Hpy188I TCNGA 456 455 . . 455 459 + DdeI CTNAG 455 458 . . 455 459 - BspCNI CTCAG 447 445 . . 455 459 - BseMII CTCAG 446 444 . . 460 464 + TspDTI ATGAA 475 473 . . 495 499 + Tsp4CI* ACNGT 497 496 . . 499 503 + Hpy188I TCNGA 501 500 . . 509 513 + BsmAI GTCTC 514 518 . . 523 527 + Hin4II* CCTTC 533 532 . . 526 529 + TaqI TCGA 526 528 . . 526 531 + Hpy178III* TCNNGA 527 529 . . 528 538 + BplI GAGNNNNNCTC 519 514 551 546 537 542 + Hpy178III* TCNNGA 538 540 . . 528 538 - BplI GAGNNNNNCTC 551 546 519 514 545 548 + SetI ASST 548 544 . . 545 548 + CviJI RGCY 546 546 . . 545 548 + AluI AGCT 546 546 . . 546 551 + Cac8I GCNNGC 548 548 . . 550 560 + MwoI GCNNNNNNNGC 556 553 . . 550 560 + BstAPI GCANNNNNTGC 556 553 . . 551 559 + AlwNI CAGNNNCTG 556 553 . . 553 557 + BsmAI GTCTC 558 562 . . 558 561 + CviRI* TGCA 559 559 . . 596 600 + Tsp4CI* ACNGT 598 597 . . 599 604 + Hpy166II GTNNAC 601 601 . . 608 611 + TaiI ACGT 611 607 . . 608 611 + SetI ASST 611 607 . . 608 611 + MaeII ACGT 608 610 . . 612 615 + CviRI* TGCA 613 613 . . 613 617 + BlsI GCNGC 615 614 . . 613 617 + BisI GCNGC 614 615 . . 613 617 + BbvI GCAGC 625 629 . . 613 617 + TseI GCWGC 613 616 . . 625 629 + BssKI CCNGG 624 629 . . 625 629 + ScrFI CCNGG 626 627 . . 625 629 + CauII* CCSGG 626 627 . . 626 629 + HpaII CCGG 626 628 . . 640 643 + MseI TTAA 640 642 . . 654 659 + PvuII CAGCTG 656 656 . . 654 659 + NspBII* CMGCKG 656 656 . . 655 658 + SetI ASST 658 654 . . 655 658 + CviJI RGCY 656 656 . . 655 658 + AluI AGCT 656 656 . . 659 664 + SduI GDGCHC 663 659 . . 659 664 + HgiJII* GRGCYC 663 659 . . 660 663 + CviJI RGCY 661 661 . . 660 665 + NlaIV GGNNCC 662 662 . . 656 661 - GsaI CCCAGC 660 656 . . 656 661 - BseYI CCCAGC 656 660 . . 664 668 + Hin4II* CCTTC 674 673 . . 664 674 + BsiYI* CCNNNNNNNGG 670 667 . . 677 681 - BccI CCATC 671 672 . . 693 696 + CviRI* TGCA 694 694 . . 694 705 + AlfI GCANNNNNNTGC 683 681 717 715 690 695 - BsrDI GCAATG 689 687 . . 699 702 + SetI ASST 702 698 . . 703 706 + CviRI* TGCA 704 704 . . 694 705 - AlfI GCANNNNNNTGC 717 715 683 681 709 713 - BccI CCATC 703 704 . . 717 720 + TspEI AATT 716 720 . . 722 727 + ApoI RAATTY 722 726 . . 723 726 + TspEI AATT 722 726 . . 745 749 + DdeI CTNAG 745 748 . . 752 757 + SecI* CCNNGG 752 756 . . 753 757 + BssKI CCNGG 752 757 . . 749 753 - BslFI GGGAC 734 738 . . 753 757 + BseBI CCWGG 754 755 . . 753 757 + ScrFI CCNGG 754 755 . . 753 757 + EcoRII CCWGG 752 757 . . 756 759 + CviJI RGCY 757 757 . . 756 759 + HaeIII GGCC 757 757 . . 758 761 + MnlI CCTC 768 767 . . 759 763 + DdeI CTNAG 759 762 . . 759 763 + BspCNI CTCAG 772 770 . . 759 763 + BseMII CTCAG 773 771 . . 760 764 + Hpy188I TCNGA 762 761 . . 763 768 + BseRI GAGGAG 778 776 . . 766 770 + PleI GAGTC 774 775 . . 763 766 - MnlI CCTC 756 755 . . 766 770 + MlyI GAGTC 775 775 . . 766 770 + HinfI GANTC 766 769 . . 772 775 + MseI TTAA 772 774 . . 808 813 + Hpy166II GTNNAC 810 810 . . 808 813 + HindII GTYRAC 810 810 . . 813 816 + FatI CATG 812 816 . . 813 816 + NlaIII CATG 816 812 . . 813 816 + CviAII CATG 813 815 . . 816 820 + FokI GGATG 829 833 . . 816 820 + BseGI GGATG 822 820 . . 819 822 + CviRI* TGCA 820 820 . . 817 821 - SfaNI GCATC 807 811 . . 830 833 + DpnI GATC 831 831 . . 830 833 + BstKTI GATC 832 830 . . 830 833 + MboI GATC 829 833 . . 832 836 + Hpy188I TCNGA 834 833 . . 835 839 + HinfI GANTC 835 838 . . 835 839 - PleI GAGTC 829 830 . . 835 839 - MlyI GAGTC 829 829 . . 847 851 + MboII GAAGA 859 858 . . 847 852 - Ksp632I* CTCTTC 842 845 . . 855 858 + SetI ASST 858 854 . . 855 858 + CviJI RGCY 856 856 . . 855 858 + AluI AGCT 856 856 . . 856 862 + EspI* GCTNAGC 857 860 . . 857 861 + DdeI CTNAG 857 860 . . 860 863 + SetI ASST 863 859 . . 860 863 + CviJI RGCY 861 861 . . 860 863 + AluI AGCT 861 861 . . 857 861 - BspCNI CTCAG 849 847 . . 857 861 - BseMII CTCAG 848 846 . . 859 869 - Hin4I GAYNNNNNVTC 882 877 850 845 875 878 + CviRI* TGCA 876 876 . . 878 888 + FalI AAGNNNNNCTT 869 864 901 896 878 888 - FalI AAGNNNNNCTT 901 896 869 864 889 893 + DdeI CTNAG 889 892 . . 893 898 + Cac8I GCNNGC 895 895 . . 898 901 + FatI CATG 897 901 . . 898 901 + NlaIII CATG 901 897 . . 898 901 + CviAII CATG 898 900 . . 923 927 + BmgT120I GGNCC 924 925 . . 923 927 + AvaII GGWCC 923 926 . . 923 927 + AsuI* GGNCC 923 926 . . 926 936 + BsiYI* CCNNNNNNNGG 932 929 . . 925 929 - TspGWI ACGGA 915 913 . . 936 941 + ApoI RAATTY 936 940 . . 937 940 + TspEI AATT 936 940 . . 940 943 + MseI TTAA 940 942 . . 947 950 + Csp6I GTAC 947 949 . . 947 950 + RsaI GTAC 948 948 . . 954 959 + SmlI CTYRAG 954 958 . . 954 959 + AflII CTTAAG 954 958 . . 955 958 + MseI TTAA 955 957 . . 959 964 + BtsI GCAGTG 966 964 . . 960 966 + TspRI CASTGNN 966 957 . . 964 969 - GsaI CCCAGC 968 964 . . 969 973 + FokI GGATG 982 986 . . 964 969 - BseYI CCCAGC 964 968 . . 969 973 + BseGI GGATG 975 973 . . 973 977 - BccI CCATC 967 968 . . 980 983 + SetI ASST 983 979 . . 978 982 - Hin4II* CCTTC 972 971 . . 982 985 + Csp6I GTAC 982 984 . . 982 985 + RsaI GTAC 983 983 . . 984 987 + TaiI ACGT 987 983 . . 984 987 + SetI ASST 987 983 . . 984 987 + MaeII ACGT 984 986 . . 996 999 + CviJI RGCY 997 997 . . 1010 1014 + TspDTI ATGAA 1025 1023 . . 1012 1017 + ApoI RAATTY 1012 1016 . . 1013 1016 + TspEI AATT 1012 1016 . . 1016 1019 + MseI TTAA 1016 1018 . . 1032 1035 + TspEI AATT 1031 1035 . . #--------------------------------------- # # # AflII: # [CTTAAG] # 96 954 # # AflIII: # [ACRYGT] # 66 984 # # AhaIII*: # [TTTAAA] # 108 942 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 333 683 # # AluI: # [AGCT] # 5 76 110 143 157 170 # 189 200 # # AlwNI: # [CAGNNNCTG] # 494 556 # # ApoI: # [RAATTY] # 38 76 214 343 379 # # AsuI*: # [GGNCC] # 127 188 735 # # AvaII: # [GGWCC] # 127 923 # # BaeI: # [ACNNNNGTAYC] # 33 153 864 # # BalI: # [TGGCCA] # 178 872 # # BbvI: # [GCAGC] # 425 625 # # BbvII*: # [GAAGAC] # 69 981 # # BccI: # [CCATC] # 32 83 264 286 385 # # BceAI: # [ACGGC] # 269 781 # # BclI: # [TGATCA] # 87 963 # # BdaI: # [TGANNNNNNTCA] # 0 0 16 34 1000 # # BinI*: # [GGATC] # 80 253 717 # # BisI: # [GCNGC] # 436 614 # # BlsI: # [GCNGC] # 435 615 # # BmgT120I: # [GGNCC] # 126 189 735 # # BplI: # [GAGNNNNNCTC] # 0 0 32 499 519 # # BseBI: # [CCWGG] # 296 754 # # BseGI: # [GGATG] # 75 153 822 # # BseMII: # [CTCAG] # 32 75 153 202 261 327 # # BseRI: # [GAGGAG] # 272 778 # # BseYI: # [CCCAGC] # 86 308 656 # # BsiI*: # [CACGAG] # 123 927 # # BsiYI*: # [CCNNNNNNNGG] # 17 118 185 262 468 # # BslFI: # [GGGAC] # 316 734 # # BsmAI: # [GTCTC] # 44 492 514 # # BsmI: # [GAATGC] # 280 770 # # BspCNI: # [CTCAG] # 34 77 152 201 261 325 # # BspHI: # [TCATGA] # 36 1014 # # BspLU11I*: # [ACATGT] # 66 984 # # BsrDI: # [GCAATG] # 212 361 477 # # BsrI: # [ACTGG] # 191 859 # # BssKI: # [CCNGG] # 128 298 624 # # BstAPI: # [GCANNNNNTGC] # 494 556 # # BstKTI: # [GATC] # 67 90 171 218 504 # # BtsI: # [GCAGTG] # 84 966 # # Cac8I: # [GCNNGC] # 155 195 347 353 # # CauII*: # [CCSGG] # 424 626 # # CfrI: # [YGGCCR] # 106 176 768 # # Csp6I: # [GTAC] # 35 68 305 642 # # CviAII: # [CATG] # 10 15 22 30 72 85 # 152 291 373 # # CviJI: # [RGCY] # 5 5 11 12 16 21 # 33 53 61 65 96 99 # 110 136 157 170 # # CviRI*: # [TGCA] # 3 10 54 56 81 116 # 174 556 # # DdeI: # [CTNAG] # 14 16 17 22 32 98 # 139 161 261 290 # # DpnI: # [GATC] # 67 89 171 219 504 # # Eam1105I: # [GACNNNNNGTC] # 435 615 # # Ecl136II: # [GAGCTC] # 157 893 # # Eco57I: # [CTGAAG] # 203 847 # # Eco57MI: # [CTGRAG] # 203 847 # # EcoRII: # [CCWGG] # 298 752 # # EspI*: # [GCTNAGC] # 193 857 # # FalI: # [AAGNNNNNCTT] # 0 0 0 0 32 32 # 149 314 523 # # FatI: # [CATG] # 10 15 21 30 72 85 # 153 291 373 # # FokI: # [GGATG] # 68 153 829 # # GlaI: # [GCGC] # 6 142 208 694 # # GsaI: # [CCCAGC] # 82 308 660 # # HaeIII: # [GGCC] # 12 94 178 293 473 # # HgiAI*: # [GWGCWC] # 159 891 # # HgiJII*: # [GRGCYC] # 159 387 504 # # HhaI: # [GCGC] # 6 142 209 693 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 168 397 # 421 # # Hin4II*: # [CCTTC] # 78 99 141 298 434 # # Hin6I: # [GCGC] # 6 142 207 695 # # HindII: # [GTYRAC] # 240 810 # # HinfI: # [GANTC] # 69 79 145 171 215 371 # # HpaII: # [CCGG] # 424 626 # # Hpy166II: # [GTNNAC] # 209 240 296 305 # # Hpy178III*: # [TCNNGA] # 11 36 37 66 123 265 # 512 # # Hpy188I: # [TCNGA] # 26 27 45 72 87 216 # 261 316 # # Hpy99I: # [CGWCG] # 290 760 # # Ksp632I*: # [CTCTTC] # 87 208 755 # # MaeII: # [ACGT] # 66 376 608 # # MaeIII: # [GTNAC] # 142 243 665 # # MboI: # [GATC] # 67 87 171 221 504 # # MboII: # [GAAGA] # 3 15 74 191 191 576 # # MlyI: # [GAGTC] # 54 139 221 636 # # MmeI: # [TCCRAC] # 271 779 # # MnlI: # [CCTC] # 12 175 234 282 347 # # MseI: # [TTAA] # 9 15 34 61 98 132 # 168 260 273 # # MwoI: # [GCNNNNNNNGC] # 494 556 # # NlaIII: # [CATG] # 10 15 25 30 72 85 # 149 291 373 # # NlaIV: # [GGNNCC] # 388 662 # # NspBII*: # [CMGCKG] # 394 656 # # NspI: # [RCATGY] # 10 70 970 # # PflMI: # [CCANNNNNTGG] # 17 185 848 # # PleI: # [GAGTC] # 55 139 221 635 # # PvuII: # [CAGCTG] # 394 656 # # RsaI: # [GTAC] # 35 67 306 642 # # SacI: # [GAGCTC] # 159 891 # # SapI: # [GCTCTTC] # 87 963 # # SauI*: # [CCTNAGG] # 433 617 # # ScrFI: # [CCNGG] # 128 296 626 # # SduI: # [GDGCHC] # 159 387 504 # # SecI*: # [CCNNGG] # 298 752 # # SetI: # [ASST] # 4 5 5 8 44 47 # 48 63 63 76 109 120 # 143 156 159 # # SfaNI: # [GCATC] # 184 218 243 405 # # SmlI: # [CTYRAG] # 96 954 # # TaiI: # [ACGT] # 63 376 611 # # TaqI: # [TCGA] # 524 526 # # TfiI: # [GAWTC] # 79 316 655 # # TseI: # [GCWGC] # 437 613 # # Tsp45I: # [GTSAC] # 142 908 # # Tsp4CI*: # [ACNGT] # 74 101 166 257 452 # # TspDTI: # [ATGAA] # 25 25 450 550 # # TspEI: # [AATT] # 6 19 19 20 27 76 # 99 109 115 214 346 # # TspGWI: # [ACGGA] # 74 135 841 # # TspRI: # [CASTGNN] # 84 147 305 514 # # TstI: # [CACNNNNNNTCC] # 32 53 965 # # XhoII: # [RGATCY] # 258 792 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1050 # Reported_sequences: 1 # Reported_hitcount: 309 #---------------------------------------