Restriction Fragments for RAD6/YGL058W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

RAD6/YGL058W on chromosome VII from coordinates 393986 to 394504.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.17570.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 12 15 + SetI ASST 15 11 . . 12 15 + CviJI RGCY 13 13 . . 12 15 + AluI AGCT 13 13 . . 14 17 + MaeI CTAG 14 16 . . 17 21 + MboII GAAGA 29 28 . . 22 25 + SetI ASST 25 21 . . 20 24 - Hin4II* CCTTC 14 13 . . 37 42 + AhaIII* TTTAAA 39 39 . . 38 41 + MseI TTAA 38 40 . . 42 45 + TaiI ACGT 45 41 . . 42 45 + SetI ASST 45 41 . . 42 45 + MaeII ACGT 42 44 . . 46 50 + TspDTI ATGAA 61 59 . . 52 56 + MboII GAAGA 64 63 . . 48 52 - Hin4II* CCTTC 42 41 . . 55 59 - SfaNI GCATC 45 49 . . 62 70 + DraIII CACNNNGTG 67 64 . . 64 68 + BssKI CCNGG 63 68 . . 64 67 + HpaII CCGG 64 66 . . 64 68 + ScrFI CCNGG 65 66 . . 64 68 + CauII* CCSGG 65 66 . . 79 83 - HphI GGTGA 71 70 . . 87 90 + SetI ASST 90 86 . . 95 98 + TaiI ACGT 98 94 . . 95 98 + SetI ASST 98 94 . . 95 98 + MaeII ACGT 95 97 . . 99 102 + FatI CATG 98 102 . . 99 102 + NlaIII CATG 102 98 . . 99 102 + CviAII CATG 99 101 . . 114 117 + FatI CATG 113 117 . . 114 117 + NlaIII CATG 117 113 . . 114 117 + CviAII CATG 114 116 . . 124 128 + BmgT120I GGNCC 125 126 . . 124 128 + AsuI* GGNCC 124 127 . . 125 128 + CviJI RGCY 126 126 . . 125 128 + HaeIII GGCC 126 126 . . 126 131 + Cac8I GCNNGC 128 128 . . 129 132 + CviJI RGCY 130 130 . . 140 145 + NdeI CATATG 141 143 . . 143 147 + TspDTI ATGAA 158 156 . . 145 149 + MboII GAAGA 157 156 . . 145 150 + BbvII* GAAGAC 152 156 . . 149 153 + TspGWI ACGGA 164 162 . . 160 163 + SetI ASST 163 159 . . 168 173 - MmeI TCCRAC 149 147 . . 179 183 + TspDTI ATGAA 194 192 . . 181 185 + MboII GAAGA 193 192 . . 197 200 + CviJI RGCY 198 198 . . 202 205 + AciI CCGC 202 204 . . 204 209 + NspI RCATGY 208 204 . . 205 208 + FatI CATG 204 208 . . 205 208 + NlaIII CATG 208 204 . . 205 208 + CviAII CATG 205 207 . . 211 216 + ApoI RAATTY 211 215 . . 212 215 + TspEI AATT 211 215 . . 222 233 + BdaI TGANNNNNNTCA 211 209 245 243 223 232 + XmnI GAANNNNTTC 227 227 . . 232 241 + MslI CAYNNNNRTG 236 236 . . 222 233 - BdaI TGANNNNNNTCA 245 243 211 209 230 234 - TspDTI ATGAA 220 218 . . 232 236 - FokI GGATG 218 222 . . 232 236 - BseGI GGATG 231 229 . . 246 249 + CviRI* TGCA 247 247 . . 253 257 + HphI GGTGA 265 264 . . 257 262 + ApoI RAATTY 257 261 . . 258 261 + TspEI AATT 257 261 . . 275 278 + CviRI* TGCA 276 276 . . 288 292 + HinfI GANTC 288 291 . . 284 288 - BccI CCATC 278 279 . . 291 296 + MmeI TCCRAC 316 314 . . 288 292 - PleI GAGTC 282 283 . . 288 292 - MlyI GAGTC 282 282 . . 296 301 + NdeI CATATG 297 299 . . 301 311 + Hin4I GAYNNNNNVTC 292 287 324 319 307 311 + SfaNI GCATC 316 320 . . 301 311 - Hin4I GAYNNNNNVTC 324 319 292 287 308 312 - FokI GGATG 294 298 . . 308 312 - BseGI GGATG 307 305 . . 320 324 - FokI GGATG 306 310 . . 320 324 - BseGI GGATG 319 317 . . 343 346 + DpnI GATC 344 344 . . 343 346 + BstKTI GATC 345 343 . . 343 352 + BsaBI GATNNNNATC 347 347 . . 343 346 + MboI GATC 342 346 . . 343 347 - BinI* GGATC 337 338 . . 354 357 + SetI ASST 357 353 . . 354 357 + CviJI RGCY 355 355 . . 354 357 + AluI AGCT 355 355 . . 355 365 + MwoI GCNNNNNNNGC 361 358 . . 346 359 - AjuI GAANNNNNNNTTGG 371 366 339 334 360 365 + Cac8I GCNNGC 362 362 . . 367 372 + AclI AACGTT 368 370 . . 368 371 + TaiI ACGT 371 367 . . 368 371 + SetI ASST 371 367 . . 368 371 + MaeII ACGT 368 370 . . 375 378 + SetI ASST 378 374 . . 375 378 + CviJI RGCY 376 376 . . 375 378 + AluI AGCT 376 376 . . 376 380 + BlsI GCNGC 378 377 . . 376 380 + BisI GCNGC 377 378 . . 376 380 + TseI GCWGC 376 379 . . 378 381 + CviRI* TGCA 379 379 . . 376 380 - BbvI GCAGC 363 367 . . 394 404 + Hin4I GAYNNNNNVTC 385 380 417 412 394 397 + DpnI GATC 395 395 . . 394 397 + BstKTI GATC 396 394 . . 394 397 + MboI GATC 393 397 . . 394 404 - Hin4I GAYNNNNNVTC 417 412 385 380 405 409 + Tsp4CI* ACNGT 407 406 . . 408 411 + Csp6I GTAC 408 410 . . 408 411 + RsaI GTAC 409 409 . . 410 413 + TaiI ACGT 413 409 . . 410 413 + SetI ASST 413 409 . . 410 413 + MaeII ACGT 410 412 . . 422 425 + MseI TTAA 422 424 . . 430 434 + Tsp4CI* ACNGT 432 431 . . 427 431 - BsmAI GTCTC 421 425 . . 427 432 - Esp3I CGTCTC 421 425 . . 450 454 + FokI GGATG 463 467 . . 450 454 + BseGI GGATG 456 454 . . 448 451 - MnlI CCTC 441 440 . . 492 496 + Hpy99I CGWCG 496 491 . . 495 499 + Hpy99I CGWCG 499 494 . . 498 502 + Hpy99I CGWCG 502 497 . . 501 505 + Hpy99I CGWCG 505 500 . . 504 508 + Hpy99I CGWCG 508 503 . . #--------------------------------------- # # # AciI: # [CCGC] # 202 317 # # AclI: # [AACGTT] # 151 368 # # AhaIII*: # [TTTAAA] # 39 480 # # AjuI: # [GAANNNNNNNTTGG] # 32 148 339 # # AluI: # [AGCT] # 13 21 143 342 # # ApoI: # [RAATTY] # 46 211 262 # # AsuI*: # [GGNCC] # 124 395 # # BbvI: # [GCAGC] # 156 363 # # BbvII*: # [GAAGAC] # 152 367 # # BccI: # [CCATC] # 241 278 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 211 274 # # BinI*: # [GGATC] # 182 337 # # BisI: # [GCNGC] # 142 377 # # BlsI: # [GCNGC] # 141 378 # # BmgT120I: # [GGNCC] # 125 394 # # BsaBI: # [GATNNNNATC] # 172 347 # # BseGI: # [GGATG] # 12 63 76 137 231 # # BsmAI: # [GTCTC] # 98 421 # # BssKI: # [CCNGG] # 63 456 # # BstKTI: # [GATC] # 51 123 345 # # Cac8I: # [GCNNGC] # 128 157 234 # # CauII*: # [CCSGG] # 65 454 # # Csp6I: # [GTAC] # 111 408 # # CviAII: # [CATG] # 15 91 99 314 # # CviJI: # [RGCY] # 4 13 21 68 113 143 # 157 # # CviRI*: # [TGCA] # 29 103 140 247 # # DpnI: # [GATC] # 51 124 344 # # DraIII: # [CACNNNGTG] # 67 452 # # Esp3I: # [CGTCTC] # 98 421 # # FatI: # [CATG] # 15 91 98 315 # # FokI: # [GGATG] # 12 56 76 157 218 # # HaeIII: # [GGCC] # 126 393 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 32 32 # 61 102 292 # # Hin4II*: # [CCTTC] # 14 28 477 # # HinfI: # [GANTC] # 231 288 # # HpaII: # [CCGG] # 64 455 # # HphI: # [GGTGA] # 71 194 254 # # Hpy166II: # [GTNNAC] # 5 514 # # Hpy99I: # [CGWCG] # 3 3 3 3 11 496 # # MaeI: # [CTAG] # 14 505 # # MaeII: # [ACGT] # 42 42 53 109 273 # # MboI: # [GATC] # 51 126 342 # # MboII: # [GAAGA] # 29 35 36 93 326 # # MlyI: # [GAGTC] # 237 282 # # MmeI: # [TCCRAC] # 149 167 203 # # MnlI: # [CCTC] # 78 441 # # MseI: # [TTAA] # 38 97 384 # # MslI: # [CAYNNNNRTG] # 236 283 # # MwoI: # [GCNNNNNNNGC] # 158 361 # # NdeI: # [CATATG] # 141 156 222 # # NlaIII: # [CATG] # 15 91 102 311 # # NspI: # [RCATGY] # 208 311 # # PleI: # [GAGTC] # 237 282 # # RsaI: # [GTAC] # 110 409 # # ScrFI: # [CCNGG] # 65 454 # # SetI: # [ASST] # 7 8 10 14 15 20 # 35 45 65 106 194 # # SfaNI: # [GCATC] # 45 203 271 # # TaiI: # [ACGT] # 42 45 53 106 273 # # TseI: # [GCWGC] # 143 376 # # Tsp4CI*: # [ACNGT] # 25 87 407 # # TspDTI: # [ATGAA] # 26 36 61 97 299 # # TspEI: # [AATT] # 46 211 262 # # TspGWI: # [ACGGA] # 164 355 # # XmnI: # [GAANNNNTTC] # 227 292 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 519 # Reported_sequences: 1 # Reported_hitcount: 127 #---------------------------------------