seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.8136EMBOSS_001 DrdI | Hin4I | Hin4I | | AclI | | MaeII | | | BccI MaeIII CviRI* | | | |SetI Tsp45I | CviJI | | | |TaiI | Hin4II* \ \ \ \ \ \\ \ \ ATGCAAAGCCCATATCCAATGACACAAGTGTCTAACGTTGATGATGGGTCACTATTGAAG 10 20 30 40 50 60 ----:----|----:----|----:----|----:----|----:----|----:----| TACGTTTCGGGTATAGGTTACTGTGTTCACAGATTGCAACTACTACCCAGTGATAACTTC / / // / // / / / | CviJI |Hin4I | |BccI | | Hin4I CviRI* |Hin4I | MaeII | | Hin4I DrdI | AclI | Tsp45I TaiI | MaeIII SetI Hin4II* M Q S P Y P M T Q V S N V D D G S L L K C K A H I Q * H K C L T L M M G H Y * R A K P I S N D T S V * R * * W V T I E G ----:----|----:----|----:----|----:----|----:----|----:----| X C L G Y G I V C T D L T S S P D S N F X A F G M D L S V L T * R Q H H T V I S H L A W I W H C L H R V N I I P * * Q L Hpy188I | KasI | HgiCI* | |AcyI TseI | |NarI AluI | |Hin6I CviJI | ||GlaI |BisI | ||DinI FatI ||BlsI | ||NlaIV |CviAII Hin4I ||SetI | |||HhaI || NspI Hin4I BbvI ||| MnlI | ||||HaeII || NlaIII \ \ \\\ \ \ \\\\\ \\ \ GAGAGTAAAAGCAAGTCCAAAGTAGCTGCGAAGTCAGAGGCGCCAAGACCACATGCTTGT 70 80 90 100 110 120 ----:----|----:----|----:----|----:----|----:----|----:----| CTCTCATTTTCGTTCAGGTTTCATCGACGCTTCAGTCTCCGCGGTTCTGGTGTACGAACA / / ///// / ///// / // BbvI | ||||MnlI | ||||HgiCI* | |FatI | |||TseI | ||||KasI | CviAII | ||BisI | |||Hin6I NlaIII | |BlsI | |||NarI NspI | CviJI | |||AcyI | AluI | ||NlaIV SetI | ||DinI | ||GlaI | |HhaI | HaeII Hpy188I E S K S K S K V A A K S E A P R P H A C R V K A S P K * L R S Q R R Q D H M L V E * K Q V Q S S C E V R G A K T T C L S ----:----|----:----|----:----|----:----|----:----|----:----| S L L L L D L T A A F D S A G L G C A Q P S Y F C T W L L Q S T L P A L V V H K L T F A L G F Y S R L * L R W S W M S T TspDTI |FatI ||CviAII ||| NlaIII ||| | ApoI ||| | TspEI AluI BsrI ||| | EcoRI CviJI | BsmAI ||| | | BdaI | SetI | | Hpy188I ||| | | BdaI \ \ \ \ \ \\\ \ \ \ CCTATCTGTCATAGAGCTTTTCACAGACTGGAACATCAGACGAGACACATGAGAATTCAT 130 140 150 160 170 180 ----:----|----:----|----:----|----:----|----:----|----:----| GGATAGACAGTATCTCGAAAAGTGTCTGACCTTGTAGTCTGCTCTGTGTACTCTTAAGTA / / / / / / / // / / | CviJI BsrI | | | | || | EcoRI | AluI | | | | || | TspEI SetI | | | | || | ApoI | | | | || BdaI | | | | || BdaI | | | | |FatI | | | | CviAII | | | NlaIII | | TspDTI | BsmAI Hpy188I P I C H R A F H R L E H Q T R H M R I H L S V I E L F T D W N I R R D T * E F I Y L S * S F S Q T G T S D E T H E N S Y ----:----|----:----|----:----|----:----|----:----|----:----| G I Q * L A K * L S S C * V L C M L I * D * R D Y L K E C V P V D S S V C S F E R D T M S S K V S Q F M L R S V H S N M CviJI | HphI BdaI | |MluI BdaI | |AflIII |BdaI | || FnuDII* |BdaI | || |OliI |BssKI | || |MslI |SecI* | || || MaeIII || HpaII XmnI MslI | || || Tsp45I || ScrFI |FokI | SetI | || || |MnlI || CauII* BseGI || SetI \ \ \ \\ \\ \\ \\ \ \ \\ \ ACAGGTGAGAAGCCTCACGCGTGTGACTTCCCCGGATGTGTGAAAAGGTTCAGTAGAAGC 190 200 210 220 230 240 ----:----|----:----|----:----|----:----|----:----|----:----| TGTCCACTCTTCGGAGTGCGCACACTGAAGGGGCCTACACACTTTTCCAAGTCATCTTCG / / / //// // /// / // / MslI | | |||MnlI |BdaI ||| BseGI |XmnI FokI SetI | | ||| |BdaI ||BssKI SetI | | ||| | |SecI* | | ||| | |HpaII | | ||| | CauII* | | ||| | ScrFI | | ||| Tsp45I | | ||| MaeIII | | ||| BdaI | | ||| BdaI | | ||AflIII | | ||MluI | | |MslI | | |OliI | | FnuDII* | HphI CviJI T G E K P H A C D F P G C V K R F S R S Q V R S L T R V T S P D V * K G S V E A R * E A S R V * L P R M C E K V Q * K R ----:----|----:----|----:----|----:----|----:----|----:----| V P S F G * A H S K G P H T F L N L L L Y L H S A E R T H S G R I H S F T * Y F C T L L R V R T V E G S T H F P E T S A MnlI | AvaI | XhoI | SmlI | AbsI BarI | BarI | BtgZI | PspXI | | TspDTI | |TaqI | | |TspDTI | |BmeT110I BdaI | | || ApoI | || MnlI BdaI | | || TspEI | || | SetI | BsmAI | | || EcoRI MboII | || | | MnlI \ \ \ \ \\ \ \ \ \\ \ \ \ GATGAACTGACGAGACACAGAAGAATTCATACAAACTCCCACCCTCGAGGTAAAAGAGGC 250 260 270 280 290 300 ----:----|----:----|----:----|----:----|----:----|----:----| CTACTTGACTGCTCTGTGTCTTCTTAAGTATGTTTGAGGGTGGGAGCTCCATTTTCTCCG / / / // / / / / // / BdaI | | || BtgZI | MboII BarI || MnlI BdaI | | |TspDTI EcoRI MnlI |PspXI | | TspDTI TspEI |AbsI | BsmAI ApoI |SmlI BarI |XhoI |AvaI |MnlI BmeT110I TaqI SetI D E L T R H R R I H T N S H P R G K R G M N * R D T E E F I Q T P T L E V K E A * T D E T Q K N S Y K L P P S R * K R Q ----:----|----:----|----:----|----:----|----:----|----:----| S S S V L C L L I * V F E W G R P L L P R H V S S V C F F E Y L S G G E L Y F L I F Q R S V S S N M C V G V R S T F S A SetI | MboII BsrI | | CviJI | AccI | | | SduI | |BssNAI Hin4II* | | | HgiJII* MaeI MaeI | |Hpy166II \ \ \ \ \ \ \ \ \\ AGAAAGAAGAAGGTTGTGGGCTCTCCAATAAATAGTGCTAGTTCTAGTGCTACCAGTATA 310 320 330 340 350 360 ----:----|----:----|----:----|----:----|----:----|----:----| TCTTTCTTCTTCCAACACCCGAGAGGTTATTTATCACGATCAAGATCACGATGGTCATAT / / // / / / / // Hin4II* SetI || CviJI MaeI MaeI BsrI |AccI |HgiJII* Hpy166II |SduI BssNAI MboII R K K K V V G S P I N S A S S S A T S I E R R R L W A L Q * I V L V L V L P V Y K E E G C G L S N K * C * F * C Y Q Y T ----:----|----:----|----:----|----:----|----:----|----:----| L F F F T T P E G I F L A L E L A V L I C F S S P Q P S E L L Y H * N * H * W Y S L L L N H A R W Y I T S T R T S G T Y MseI HphI |SwaI |ApoI AciI MseI |AhaIII* |TspEI |BceAI SetI |TspEI \\ \\ \\ \ \\ CCAGATTTAAATACGGCAAATTTTTCACCGCCATTACCACAGCAACACCTATCGCCTTTA 370 380 390 400 410 420 ----:----|----:----|----:----|----:----|----:----|----:----| GGTCTAAATTTATGCCGTTTAAAAAGTGGCGGTAATGGTGTCGTTGTGGATAGCGGAAAT // / / // / / |MseI HphI TspEI |BceAI SetI MseI AhaIII* ApoI AciI SwaI P D L N T A N F S P P L P Q Q H L S P L Q I * I R Q I F H R H Y H S N T Y R L * R F K Y G K F F T A I T T A T P I A F N ----:----|----:----|----:----|----:----|----:----|----:----| G S K F V A F K E G G N G C C C R D G K V L N L Y P L N K V A M V V A V G I A K W I * I R C I K * R W * W L L V * R R * Hpy188I | FalI | FalI | | ApoI | | TspEI | | | MboII | | | | TaqI | | | | |MboI FalI | | | | || DpnI FalI | | | | || |BstKTI |SetI \ \ \ \ \\ \\ \\ ATTCCTATTGCTATTGCTCCGAAAGAAAATTCAAGTCGATCTTCTACAAGAAAAGGTAGA 430 440 450 460 470 480 ----:----|----:----|----:----|----:----|----:----|----:----| TAAGGATAACGATAACGAGGCTTTCTTTTAAGTTCAGCTAGAAGATGTTCTTTTCCATCT / / / // / / / TspEI Hpy188I TspEI || MboI | SetI FalI MboII |DpnI FalI FalI ApoI BstKTI FalI TaqI I P I A I A P K E N S S R S S T R K G R F L L L L L R K K I Q V D L L Q E K V E S Y C Y C S E R K F K S I F Y K K R * K ----:----|----:----|----:----|----:----|----:----|----:----| I G I A I A G F S F E L R D E V L F P L L E * Q * Q E S L F N L D I K * L F L Y N R N S N S R F F I * T S R R C S F T S ApoI TspEI | TaqI MboII TsoI | AsuII |BsiYI* | BccI \ \ \\ \ \ AAAACCAAATTCGAAATCGGCGAAAGTGGTGGGAATGACCCATATATGGTTTCTTCTCCC 490 500 510 520 530 540 ----:----|----:----|----:----|----:----|----:----|----:----| TTTTGGTTTAAGCTTTAGCCGCTTTCACCACCCTTACTGGGTATATACCAAAGAAGAGGG / / // / | AsuII |MboII TsoI | TaqI BsiYI* TspEI ApoI K T K F E I G E S G G N D P Y M V S S P K P N S K S A K V V G M T H I W F L L P N Q I R N R R K W W E * P I Y G F F S Q ----:----|----:----|----:----|----:----|----:----|----:----| F V L N S I P S L P P F S G Y I T E E G F F W I R F R R F H H S H G M Y P K K E F G F E F D A F T T P I V W I H N R R G BstXI | CviJI | |DdeI HphI | || TfiI BsmAI | SetI Hin4II* | || HinfI Esp3I CviJI | | MnlI | TspRI \ \\ \ \ \ \ \ \ \ \ AAAACGATGGCTAAGATTCCCGTCTCGGTGAAGCCTCCACCTTCTTTAGCACTGAATAAT 550 560 570 580 590 600 ----:----|----:----|----:----|----:----|----:----|----:----| TTTTGCTACCGATTCTAAGGGCAGAGCCACTTCGGAGGTGGAAGAAATCGTGACTTATTA // / / / / / / / / / |BstXI | | HinfI | | HphI | | Hin4II* BccI | | TfiI | | SetI | TspRI | DdeI | CviJI MnlI CviJI Esp3I BsmAI K T M A K I P V S V K P P P S L A L N N K R W L R F P S R * S L H L L * H * I I N D G * D S R L G E A S T F F S T E * Y ----:----|----:----|----:----|----:----|----:----|----:----| L V I A L I G T E T F G G G E K A S F L W F S P * S E R R P S A E V K K L V S Y F R H S L N G D R H L R W R R * C Q I I BtsI | MwoI BseGI | |MboII FokI TspDTI | |BbvII* BglI | TspDTI | AciI | || TspRI CviJI MwoI \ \ \ \ \ \\ \ \ \ ATGAACTACCAAACTTCATCCGCTTCCACTGCTTTGTCTTCGTTGAGCAATAGCCATAGT 610 620 630 640 650 660 ----:----|----:----|----:----|----:----|----:----|----:----| TACTTGATGGTTTGAAGTAGGCGAAGGTGACGAAACAGAAGCAACTCGTTATCGGTATCA / / / / // / / / / | FokI TspDTI | || | BbvII* | MwoI TspDTI BseGI | || MboII | BglI | |MwoI CviJI | TspRI | BtsI AciI M N Y Q T S S A S T A L S S L S N S H S * T T K L H P L P L L C L R * A I A I V E L P N F I R F H C F V F V E Q * P * W ----:----|----:----|----:----|----:----|----:----|----:----| I F * W V E D A E V A K D E N L L L W L Y S S G F K M R K W Q K T K T S C Y G Y H V V L S * G S G S S Q R R Q A I A M T AccI AcyI |TsoI | BsrDI |Hpy166II | | HgaI || BslFI | | |NheI || | MluI | | ||MaeI || | AflIII | | |||Cac8I || | | FnuDII* | | |||| BmtI \\ \ \ \ \ \ \\\\ \ GGCAGTAGACTGAAACTGAACGCGTTATCGTCCCTACAAATGATGACGCCCATTGCTAGC 670 680 690 700 710 720 ----:----|----:----|----:----|----:----|----:----|----:----| CCGTCATCTGACTTTGACTTGCGCAATAGCAGGGATGTTTACTACTGCGGGTAACGATCG / // / / / / / /// | |AccI | | AflIII BsrDI | ||HgaI | Hpy166II | | MluI AcyI | ||NheI TsoI | FnuDII* | |MwoI BslFI | |MaeI | Cac8I | TspRI BmtI G S R L K L N A L S S L Q M M T P I A S A V D * N * T R Y R P Y K * * R P L L A Q * T E T E R V I V P T N D D A H C * Q ----:----|----:----|----:----|----:----|----:----|----:----| P L L S F S F A N D D R C I I V G M A L H C Y V S V S R T I T G V F S S A W Q * A T S Q F Q V R * R G * L H H R G N S A MwoI | Hin6I | |GlaI | ||HhaI | ||BtsI | ||TspRI AsuI* | |||StyI AvaII | |||SecI* Tsp4CI* | |||| TspDTI |BmgT120I ApoI | |||| | Tsp4CI* || Hpy178III* TspEI \ \\\\ \ \ \\ \ \ AGTGCGCCAAGGACTGTTTTCATAGACGGTCCTGAACAGAAACAACTACAACAACAACAA 730 740 750 760 770 780 ----:----|----:----|----:----|----:----|----:----|----:----| TCACGCGGTTCCTGACAAAAGTATCTGCCAGGACTTGTCTTTGTTGATGTTGTTGTTGTT //// / / / // / |||| | Tsp4CI* | || Hpy178III* |||| SecI* | |AvaII |||| StyI | |AsuI* |||TspDTI | BmgT120I ||Hin6I Tsp4CI* |GlaI BtsI HhaI S A P R T V F I D G P E Q K Q L Q Q Q Q V R Q G L F S * T V L N R N N Y N N N K C A K D C F H R R S * T E T T T T T T K ----:----|----:----|----:----|----:----|----:----|----:----| L A G L V T K M S P G S C F C S C C C C C H A L S Q K * L R D Q V S V V V V V V T R W P S N E Y V T R F L F L * L L L L StyI SecI* | CviJI | HaeIII | |AciI | |BisI | ||BlsI | ||MmeI | |||TauI | |||FnuDII* | ||||MboI MaeII | ||||| DpnI | SetI Tsp4CI* | ||||| |BstKTI HphI | TaiI | TspRI | ||||| || MseI \ \ \ \ \ \ \\\\\ \\ \ AATTCTCTTTCACCACGTTATTCCAACACTGTTATATTACCAAGGCCGCGATCTTTAACG 790 800 810 820 830 840 ----:----|----:----|----:----|----:----|----:----|----:----| TTAAGAGAAAGTGGTGCAATAAGGTTGTGACAATATAATGGTTCCGGCGCTAGAAATTGC / / / / / / ///// // / / | TspEI | MaeII | Tsp4CI* ||||| || | MseI | ApoI TaiI TspRI ||||| || MboI HphI SetI ||||| |DpnI ||||| BstKTI ||||FnuDII* ||||AciI |||BisI ||BlsI |HaeIII |CviJI |MmeI |TauI SecI* StyI N S L S P R Y S N T V I L P R P R S L T I L F H H V I P T L L Y Y Q G R D L * R F S F T T L F Q H C Y I T K A A I F N G ----:----|----:----|----:----|----:----|----:----|----:----| F E R E G R * E L V T I N G L G R D K V F N E K V V N N W C Q * I V L A A I K L I R K * W T I G V S N Y * W P R S R * R DdeI | BsmAI | Hpy188I | | SduI | | HgiAI* | | | TspGWI | | | | DdeI | | | | BspCNI TspGWI CviRI* | | | | |BseMII \ \ \ \ \ \ \\ GATTTTCAAGGATTGAACAATGCAAATCCAAACAACAATGGAAGTCTCAGAGCACAAACT 850 860 870 880 890 900 ----:----|----:----|----:----|----:----|----:----|----:----| CTAAAAGTTCCTAACTTGTTACGTTTAGGTTTGTTGTTACCTTCAGAGTCTCGTGTTTGA / / /// / / // TspGWI CviRI* ||| | | |BseMII ||| | | BspCNI ||| | TspGWI ||| BsmAI ||HgiAI* ||SduI |DdeI Hpy188I D F Q G L N N A N P N N N G S L R A Q T I F K D * T M Q I Q T T M E V S E H K L F S R I E Q C K S K Q Q W K S Q S T N S ----:----|----:----|----:----|----:----|----:----|----:----| S K * P N F L A F G F L L P L R L A C V P N E L I S C H L D L C C H F D * L V F I K L S Q V I C I W V V I S T E S C L S Csp6I |RsaI ||PpiI ||BspCNI |||BseMII |||Ksp632I* ||||Tsp4CI* |||||BsmAI AjuI Hpy188I |||||Eco31I MboII MseI PpiI CfrI \ \\\\\\ \ \ \ \ CAGAGTTCCGTACAGTTGAAGAGACCAAGTTCAGTTTTAAGTTTGAACGACTTGTTGGTT 910 920 930 940 950 960 ----:----|----:----|----:----|----:----|----:----|----:----| GTCTCAAGGCATGTCAACTTCTCTGGTTCAAGTCAAAATTCAAACTTGCTGAACAACCAA // / //// / / / / / |DdeI | |||| | Eco31I | AjuI PpiI | | |||| | BsmAI MboII MseI | | |||| Ksp632I* | | |||Tsp4CI* | | ||Csp6I | | |BseMII | | |RsaI | | BspCNI | PpiI Hpy188I Q S S V Q L K R P S S V L S L N D L L V R V P Y S * R D Q V Q F * V * T T C W L E F R T V E E T K F S F K F E R L V G W ----:----|----:----|----:----|----:----|----:----|----:----| * L E T C N F L G L E T K L K F S K N T E S N R V T S S V L N L K L N S R S T P L T G Y L Q L S W T * N * T Q V V Q Q N MlyI PleI TfiI HinfI BalI | Hpy188I CviJI | |HinfI MnlI BseGI HaeIII AjuI | || Hpy188I | BsrI |HphI \ \ \ \\ \ \ \ \\ GGCCAAAGAAATACCAACGAATCTGACTCTGATTTTACTACTGGTGGTGAGGATGAAGAA 970 980 990 1000 1010 1020 ----:----|----:----|----:----|----:----|----:----|----:----| CCGGTTTCTTTATGGTTGCTTAGACTGAGACTAAAATGATGACCACCACTCCTACTTCTT / / // // // / / / / | AjuI || || |Hpy188I | BsrI | HphI | CfrI || || HinfI MnlI BseGI HaeIII || |Hpy188I CviJI || HinfI BalI || TfiI |PleI MlyI G Q R N T N E S D S D F T T G G E D E E A K E I P T N L T L I L L L V V R M K K P K K Y Q R I * L * F Y Y W W * G * R R ----:----|----:----|----:----|----:----|----:----|----:----| P W L F V L S D S E S K V V P P S S S S Q G F F Y W R I Q S Q N * * Q H H P H L A L S I G V F R V R I K S S T T L I F F FokI |BbvII* || MboII || |TspDTI || || MboII || || | BaeI || || | AsuI* || || | AvaII || || | DraII || || | PpuMI || || | |BmgT120I SmlI || || | ||NlaIV TaqI |SetI || || | ||| TspGWI MaeI ClaI || BaeI CviRI* \\ \\ \ \\\ \ \ \ \\ \ \ GACGGACTAAAGGACCCGTCTAACTCTAGTATCGATAACCTTGAGCAAGACTATTTGCAA 1030 1040 1050 1060 1070 1080 ----:----|----:----|----:----|----:----|----:----|----:----| CTGCCTGATTTCCTGGGCAGATTGAGATCATAGCTATTGGAACTCGTTCTGATAAACGTT //// // / / / / / / / |||| |TspGWI MaeI | | | SmlI | Bce83I* |||| |PpuMI | | BaeI CviRI* |||| |DraII | SetI |||| |AvaII ClaI |||| |AsuI* TaqI |||| BmgT120I |||| NlaIV |||BbvII* |||MboII ||FokI |BaeI TspDTI MboII D G L K D P S N S S I D N L E Q D Y L Q T D * R T R L T L V S I T L S K T I C K R T K G P V * L * Y R * P * A R L F A R ----:----|----:----|----:----|----:----|----:----|----:----| S P S F S G D L E L I S L R S C S * K C L R V L P G T * S * Y R Y G Q A L S N A V S * L V R R V R T D I V K L L V I Q L DdeI |BinI* || MboI || XhoII || | DpnI || | |BstKTI Bce83I* || | || SpeI | Hpy178III* DdeI MslI || | || |MaeI \ \ \ \ \\ \ \\ \\ GAGCAATCAAGAAAGAAATCTAAGACTTCCACGCCCACGACAATGCTAAGTAGATCCACT 1090 1100 1110 1120 1130 1140 ----:----|----:----|----:----|----:----|----:----|----:----| CTCGTTAGTTCTTTCTTTAGATTCTGAAGGTGCGGGTGCTGTTACGATTCATCTAGGTGA / / / // // / Hpy178III* DdeI MslI || || XhoII || || MboI || |DpnI || BstKTI |DdeI BinI* E Q S R K K S K T S T P T T M L S R S T S N Q E R N L R L P R P R Q C * V D P L A I K K E I * D F H A H D N A K * I H * ----:----|----:----|----:----|----:----|----:----|----:----| S C D L F F D L V E V G V V I S L L D V L A I L F S I * S K W A W S L A L Y I W L L * S L F R L S G R G R C H * T S G S Csp6I |RsaI BseRI || ApoI |TspDTI || TspEI CviRI* || CviRI* \\ \ \ \\ \ AGTGGTACGAATTTGCACACTTTGGGGTATGTAATGAACCAAAATCACTTGCATTTCTCC 1150 1160 1170 1180 1190 1200 ----:----|----:----|----:----|----:----|----:----|----:----| TCACCATGCTTAAACGTGTGAAACCCCATACATTACTTGGTTTTAGTGAACGTAAAGAGG // // / / // / || |Csp6I | CviRI* || CviRI* || RsaI TspEI |TspDTI |SpeI ApoI BseRI MaeI S G T N L H T L G Y V M N Q N H L H F S V V R I C T L W G M * * T K I T C I S P W Y E F A H F G V C N E P K S L A F L L ----:----|----:----|----:----|----:----|----:----|----:----| L P V F K C V K P Y T I F W F * K C K E * H Y S N A C K P T H L S G F D S A N R T T R I Q V S Q P I Y H V L I V Q M E G AclI MaeII MnlI | SetI |Hpy178III* | TaiI TseI \\ \ \ \ TCATCATCTCCTGATTTCCAAAAGGAGTTGAACAACAGATTACTGAACGTTCAACAACAG 1210 1220 1230 1240 1250 1260 ----:----|----:----|----:----|----:----|----:----|----:----| AGTAGTAGAGGACTAAAGGTTTTCCTCAACTTGTTGTCTAATGACTTGCAAGTTGTTGTC / / / / | Hpy178III* | MaeII MnlI | AclI TaiI SetI S S S P D F Q K E L N N R L L N V Q Q Q H H L L I S K R S * T T D Y * T F N N S I I S * F P K G V E Q Q I T E R S T T A ----:----|----:----|----:----|----:----|----:----|----:----| E D D G S K W F S N F L L N S F T * C C R M M E Q N G F P T S C C I V S R E V V * * R R I E L L L Q V V S * Q V N L L L BisI MaeII |BlsI CviRI* | SetI || MwoI BbvI | EcoP15I | TaiI \\ \ \ \ \ \ \ CAGCAAGAGCAACATACCCTACTGCAATCACAAAATACGTCAAACCAAAGTCAAAATCAA 1270 1280 1290 1300 1310 1320 ----:----|----:----|----:----|----:----|----:----|----:----| GTCGTTCTCGTTGTATGGGATGACGTTAGTGTTTTATGCAGTTTGGTTTCAGTTTTAGTT /// / / / / / ||MwoI BbvI | | | MaeII ||TseI | | TaiI |BisI | | SetI BlsI | EcoP15I CviRI* Q Q E Q H T L L Q S Q N T S N Q S Q N Q S K S N I P Y C N H K I R Q T K V K I K A R A T Y P T A I T K Y V K P K S K S K ----:----|----:----|----:----|----:----|----:----|----:----| C C S C C V R S C D C F V D F W L * F * A A L A V Y G V A I V F Y T L G F D F D L L L L M G * Q L * L I R * V L T L I L MseI | TatI CviJI | |Csp6I MaeIII BccI | BsrI | ||RsaI HphI Tsp45I \ \ \ \ \\\ \ \ AATCAAAATCAAATGATGGCTTCCAGTAGTTCGTTAAGTACAACCCCGTTATTATTGTCA 1330 1340 1350 1360 1370 1380 ----:----|----:----|----:----|----:----|----:----|----:----| TTAGTTTTAGTTTACTACCGAAGGTCATCAAGCAATTCATGTTGGGGCAATAATAACAGT / / / / /// / BccI | BsrI | ||TatI HphI CviJI | |Csp6I | RsaI MseI N Q N Q M M A S S S S L S T T P L L L S I K I K * W L P V V R * V Q P R Y Y C H S K S N D G F Q * F V K Y N P V I I V T ----:----|----:----|----:----|----:----|----:----|----:----| F * F * I I A E L L E N L V V G N N N D F D F D F S P K W Y N T L Y L G T I I T I L I L H H S G T T R * T C G R * * Q * MseI StyI VspI TaqII SecI* |HphI MmeI | DdeI Hpy188I \ \\ \ \ \ \ CCAAGGGTGAATATGATTAATACTGCTATATCCACCCAACAAACCCCCATTTCTCAGTCG 1390 1400 1410 1420 1430 1440 ----:----|----:----|----:----|----:----|----:----|----:----| GGTTCCCACTTATACTAATTATGACGATATAGGTGGGTTGTTTGGGGGTAAAGAGTCAGC / / / / / / / / | SecI* | VspI MmeI TaqII | Hpy188I | StyI | MseI DdeI Tsp45I HphI MaeIII P R V N M I N T A I S T Q Q T P I S Q S Q G * I * L I L L Y P P N K P P F L S R K G E Y D * Y C Y I H P T N P H F S V G ----:----|----:----|----:----|----:----|----:----|----:----| G L T F I I L V A I D V W C V G M E * D V L P S Y S * Y Q * I W G V F G W K E T W P H I H N I S S Y G G L L G G N R L R TfiI HinfI | BspCNI | |BseMII Hpy166II | || Hpy178III* | TaqII | || | BsrI | | Tsp4CI* \ \\ \ \ \ \ \ GATTCACAAGTTCAAGAACTGGAAACATTACCACCCATAAGAAGTTTACCGTTGCCCTTC 1450 1460 1470 1480 1490 1500 ----:----|----:----|----:----|----:----|----:----|----:----| CTAAGTGTTCAAGTTCTTGACCTTTGTAATGGTGGGTATTCTTCAAATGGCAACGGGAAG // / / / / |BseMII | BsrI | Tsp4CI* BspCNI Hpy178III* Hpy166II HinfI TaqII TfiI D S Q V Q E L E T L P P I R S L P L P F I H K F K N W K H Y H P * E V Y R C P S F T S S R T G N I T T H K K F T V A L P ----:----|----:----|----:----|----:----|----:----|----:----| S E C T * S S S V N G G M L L K G N G K P N V L E L V P F M V V W L F N V T A R I * L N L F Q F C * W G Y S T * R Q G E FatI |CviAII |BsiYI* |Hin4II* || NlaIII \\ \ CCACACATGGACTGA 1510 ----:----|----: GGTGTGTACCTGACT / / // | | |FatI | | CviAII | Hin4II* | NlaIII BsiYI* P H M D * H T W T X T H G L X ----:----|----: G C M S Q G V C P S W V H V S # Enzymes that cut Frequency Isoschizomers AbsI 1 AccI 2 FblI,XmiI AciI 3 BspACI,SsiI AclI 2 Psp1406I AcyI 2 BsaHI,BssNI,BstACI,Hin1I,Hsp92I AflIII 2 AhaIII* 1 DraI AjuI 1 AluI 2 AluBI ApoI 7 AcsI,XapI AsuI* 2 Cfr13I,PspPI,Sau96I,AspS9I AsuII 1 Bpu14I,Bsp119I,BspT104I,BstBI,Csp45I,NspV,SfuI AvaI 1 Ama87I,BsiHKCI,BsoBI,Eco88I AvaII 2 Bme18I,Eco47I,SinI,VpaK11BI BaeI 1 BalI 1 MlsI,MluNI,MscI,Msp20I BarI 1 BbvI 2 BseXI,BstV1I,Lsp1109I BbvII* 2 BpiI,BpuAI,BstV2I,BbsI BccI 3 Bce83I* 1 BpuEI BceAI 1 BdaI 4 BglI 1 BinI* 1 AlwI,BspPI,AclWI BisI 3 Fnu4HI,Fsp4HI,GluI,ItaI,SatI BlsI 3 BmeT110I 1 BmgT120I 2 BmtI 1 BspOI BseGI 3 BstF5I,BtsCI BseMII 3 BseRI 1 BsiYI* 2 Bsc4I,BseLI,BslI,AfiI BslFI 1 BsmFI,FaqI BsmAI 5 Alw26I,BstMAI BspCNI 3 BsrDI 1 BseMI,Bse3DI BsrI 5 BseNI,Bse1I,BsrSI BssKI 1 BstSCI,StyD4I BssNAI 1 Bst1107I,BstZ17I BstKTI 3 BstXI 1 BtgZI 1 BtsI 2 Cac8I 1 BstC8I CauII* 1 BcnI,BpuMI,NciI,AsuC2I CfrI 1 AcoI,EaeI ClaI 1 Bsa29I,BseCI,BshVI,BspDI,BspXI,Bsu15I,BsuTUI,BanIII Csp6I 3 CviQI,RsaNI CviAII 3 CviJI 11 CviKI-1 CviRI* 6 HpyCH4V DdeI 6 BstDEI,HpyF3I DinI 1 EgeI,EheI,SfoI DpnI 3 MalI DraII 1 EcoO109I DrdI 1 AasI,DseDI Eco31I 1 Bso31I,BspTNI,BsaI EcoP15I 1 EcoRI 2 Esp3I 1 BsmBI FalI 2 FatI 3 FnuDII* 3 Bsh1236I,BspFNI,BstFNI,BstUI,MvnI,AccII FokI 3 GlaI 2 HaeII 1 BstH2I HaeIII 2 BsnI,BsuRI,BshFI,PhoI HgaI 1 CseI HgiAI* 1 Bbv12I,BsiHKAI,Alw21I HgiCI* 1 BanI,BshNI,BspT107I,AccB1I HgiJII* 1 Eco24I,EcoT38I,FriOI,BanII HhaI 2 BstHHI,CfoI,AspLEI Hin4I 2 Hin4II* 4 HpyAV Hin6I 2 HinP1I,HspAI HinfI 4 HpaII 1 HapII,BsiSI,MspI HphI 7 AsuHPI Hpy166II 3 Hpy8I Hpy178III* 4 Hpy188III Hpy188I 8 KasI 1 Ksp632I* 1 Eam1104I,EarI,Bst6I MaeI 5 FspBI,BfaI,XspI MaeII 4 HpyCH4IV MaeIII 3 MboI 3 Bsp143I,BssMI,BstMBI,DpnII,Kzo9I,BfuCI,NdeII,Sau3AI MboII 8 MluI 2 MlyI 1 SchI MmeI 2 MnlI 8 MseI 6 Tru1I,Tru9I MslI 3 RseI,SmiMI MwoI 4 HpyF10VI,BstMWI NarI 1 Mly113I NheI 1 AsuNHI NlaIII 3 Hin1II,Hsp92II,FaeI NlaIV 2 BspLI,BmiI,PspN4I NspI 1 BstNSI,XceI OliI 1 AleI PleI 1 PpsI PpiI 1 PpuMI 1 Psp5II,PspPPI PspXI 1 RsaI 3 AfaI ScrFI 1 BmrFI,MspR9I,Bme1390I SduI 2 MhlI,Bsp1286I SecI* 4 BseDI,BssECI,BsaJI SetI 14 SmlI 2 SmoI SpeI 1 BcuI,AhlI StyI 3 Eco130I,EcoT14I,ErhI,BssT1I SwaI 1 SmiI TaiI 4 TaqI 4 TaqII 2 TatI 1 TauI 1 TfiI 3 PfeI TseI 2 ApeKI TsoI 2 Tsp45I 3 NmuCI Tsp4CI* 5 HpyCH4III,TaaI,Bst4CI TspDTI 8 TspEI 8 TasI,Tsp509I,Sse9I TspGWI 3 TspRI 4 TscAI VspI 1 PshBI,AseI XhoI 1 Sfr274I,SlaI,StrI,TliI,PaeR7I XhoII 1 BstYI,MflI,PsuI,BstX2I XmnI 1 MroXI,PdmI,Asp700I # Enzymes which cut less frequently than the MINCUTS criterion # Enzymes < MINCUTS Frequency Isoschizomers # Enzymes which cut more frequently than the MAXCUTS criterion # Enzymes > MAXCUTS Frequency Isoschizomers # Enzymes that do not cut AarI AatII Acc65I AflII AgeI AlfI AloI AlwNI ApaI ApaLI AscI Asp718I AvrII BamHI BbvCI BcgI BciVI BclI BetI* BfiI BglII BplI Bpu10I BsaAI BsaBI BsaXI BseBI BsePI BseSI BseYI BsgI BsiI* BsmI Bsp120I Bsp1407I BspHI BspLU11I* BspMI BspMII* BsrBI Bst2UI BstAPI BstEII BstNI BstOI BtrI Cfr10I Cfr9I CspCI DraIII DsaI* Eam1105I EciI Ecl136II Eco47III Eco57I Eco57MI EcoICRI EcoNI EcoRII EcoRV EcoT22I EspI* FauI FseI FspAI GsaI GsuI HindII HindIII HpaI Hpy99I KpnI MauBI McrI* MfeI Mph1103I MroNI MstI* MvaI NaeI NcoI NdeI NgoMIV NmeAIII NotI NruI NsiI NspBII* PacI PasI PflMI PfoI PmaCI PmeI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SauI* ScaI SexAI SfaNI SfeI* SfiI SgfI SgrAI SgrDI SmaI SnaBI SphI SplI* SrfI Sse232I* Sse8387I SspI StuI TspMI TstI Tth111I XbaI XcmI XmaCI XmaI XmaIII* ZraI Zsp2I # No. of cutting enzymes which do not match the # SITELEN, BLUNT, STICKY, COMMERCIAL, AMBIGUOUS criteria 769