Restriction Fragments for RSC8/YFR037C



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The currently selected gene/sequence is :

RSC8/YFR037C on chromosome VI from coordinates 229186 to 227513.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.5693.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 15 + TspRI CASTGNN 15 6 . . 24 28 + FokI GGATG 37 41 . . 24 28 + BseGI GGATG 30 28 . . 28 32 - BslFI GGGAC 13 17 . . 37 42 + Hpy166II GTNNAC 39 39 . . 37 42 + AccI GTMKAC 38 40 . . 40 44 + HinfI GANTC 40 43 . . 40 44 - PleI GAGTC 34 35 . . 40 44 - MlyI GAGTC 34 34 . . 53 58 + McrI* CGRYCG 56 54 . . 54 59 + TaqII GACCGA 70 68 . . 56 61 + SecI* CCNNGG 56 60 . . 58 63 + BseRI GAGGAG 73 71 . . 60 65 + NlaIV GGNNCC 62 62 . . 58 61 - MnlI CCTC 51 50 . . 62 65 + CviJI RGCY 63 63 . . 65 70 + TaqII CACCCA 81 79 . . 88 92 + BccI CCATC 96 97 . . 97 100 + MnlI CCTC 107 106 . . 102 106 + DdeI CTNAG 102 105 . . 120 124 - Hin4II* CCTTC 114 113 . . 127 131 + TfiI GAWTC 127 130 . . 124 127 - MnlI CCTC 117 116 . . 127 131 + HinfI GANTC 127 130 . . 145 155 + MwoI GCNNNNNNNGC 151 148 . . 142 152 - BsaXI ACNNNNNCTCC 164 161 134 131 153 156 + SetI ASST 156 152 . . 153 156 + CviJI RGCY 154 154 . . 153 156 + AluI AGCT 154 154 . . 169 172 + TspEI AATT 168 172 . . 187 195 + AlwNI CAGNNNCTG 192 189 . . 192 196 + BsrI ACTGG 197 195 . . 193 198 + Eco57MI CTGRAG 214 212 . . 193 198 + GsuI CTGGAG 214 212 . . 196 201 + BseRI GAGGAG 211 209 . . 196 199 - MnlI CCTC 189 188 . . 203 209 + DraII RGGNCCY 204 207 . . 204 207 + CviJI RGCY 205 205 . . 204 207 + HaeIII GGCC 205 205 . . 204 208 + BmgT120I GGNCC 205 206 . . 204 208 + AsuI* GGNCC 204 207 . . 201 205 - Hin4II* CCTTC 195 194 . . 207 213 + SauI* CCTNAGG 208 211 . . 208 212 + DdeI CTNAG 208 211 . . 211 214 + SetI ASST 214 210 . . 212 217 + NlaIV GGNNCC 214 214 . . 216 220 + BssKI CCNGG 215 220 . . 216 220 + BseBI CCWGG 217 218 . . 216 220 + ScrFI CCNGG 217 218 . . 216 220 + EcoRII CCWGG 215 220 . . 232 240 + DraIII CACNNNGTG 237 234 . . 234 238 + BssKI CCNGG 233 238 . . 234 238 + ScrFI CCNGG 235 236 . . 234 238 + CauII* CCSGG 235 236 . . 235 238 + HpaII CCGG 235 237 . . 231 235 - HphI GGTGA 223 222 . . 237 241 + HphI GGTGA 249 248 . . 248 252 + Hpy99I CGWCG 252 247 . . 246 250 - TspGWI ACGGA 236 234 . . 257 260 + MnlI CCTC 267 266 . . 273 278 + AsuII TTCGAA 274 276 . . 274 277 + TaqI TCGA 274 276 . . 276 280 + MboII GAAGA 288 287 . . 278 283 + XhoII RGATCY 278 282 . . 279 282 + DpnI GATC 280 280 . . 279 282 + BstKTI GATC 281 279 . . 279 282 + MboI GATC 278 282 . . 283 288 + BsiI* CACGAG 283 287 . . 279 283 - BinI* GGATC 273 274 . . 288 291 + DpnI GATC 289 289 . . 288 291 + BstKTI GATC 290 288 . . 288 291 + MboI GATC 287 291 . . 290 293 + TaqI TCGA 290 292 . . 298 303 + XhoII RGATCY 298 302 . . 299 302 + DpnI GATC 300 300 . . 299 302 + BstKTI GATC 301 299 . . 299 302 + MboI GATC 298 302 . . 299 303 - BinI* GGATC 293 294 . . 306 311 + Hpy178III* TCNNGA 307 309 . . 322 326 + TfiI GAWTC 322 325 . . 322 326 + HinfI GANTC 322 325 . . 324 328 - TspDTI ATGAA 314 312 . . 331 334 + SetI ASST 334 330 . . 335 340 + Hpy178III* TCNNGA 336 338 . . 366 371 + ApoI RAATTY 366 370 . . 367 370 + TspEI AATT 366 370 . . 380 385 + AflIII ACRYGT 380 384 . . 381 386 + BsaAI YACGTR 383 383 . . 382 385 + TaiI ACGT 385 381 . . 382 385 + SetI ASST 385 381 . . 382 385 + MaeII ACGT 382 384 . . 384 387 + Csp6I GTAC 384 386 . . 384 387 + RsaI GTAC 385 385 . . 386 390 + Tsp4CI* ACNGT 388 387 . . 398 403 + SplI* CGTACG 398 402 . . 399 402 + Csp6I GTAC 399 401 . . 399 402 + RsaI GTAC 400 400 . . 396 400 - BceAI ACGGC 381 383 . . 404 409 + SspI AATATT 406 406 . . 419 424 + NspBII* CMGCKG 421 421 . . 419 422 + AciI CCGC 419 421 . . 428 432 + MboII GAAGA 440 439 . . 440 445 + DsaI* CCRYGG 440 444 . . 440 445 + StyI CCWWGG 440 444 . . 440 445 + NcoI CCATGG 440 444 . . 440 445 + SecI* CCNNGG 440 444 . . 441 444 + FatI CATG 440 444 . . 441 444 + NlaIII CATG 444 440 . . 441 444 + CviAII CATG 441 443 . . 444 448 + FokI GGATG 457 461 . . 444 448 + BseGI GGATG 450 448 . . 445 455 + Hin4I GAYNNNNNVTC 436 431 468 463 452 455 + MnlI CCTC 462 461 . . 454 457 + TaqI TCGA 454 456 . . 462 466 + MboII GAAGA 474 473 . . 465 469 + TfiI GAWTC 465 468 . . 465 469 + HinfI GANTC 465 468 . . 470 475 + MluI ACGCGT 470 474 . . 470 475 + AflIII ACRYGT 470 474 . . 471 474 + FnuDII* CGCG 472 472 . . 490 493 + CviJI RGCY 491 491 . . 493 496 + MseI TTAA 493 495 . . 499 502 + TspEI AATT 498 502 . . 504 508 + Hpy188I TCNGA 506 505 . . 514 519 + SecI* CCNNGG 514 518 . . 515 519 + BssKI CCNGG 514 519 . . 515 519 + BseBI CCWGG 516 517 . . 515 519 + ScrFI CCNGG 516 517 . . 515 519 + EcoRII CCWGG 514 519 . . 518 522 + BmgT120I GGNCC 519 520 . . 518 522 + AvaII GGWCC 518 521 . . 518 522 + AsuI* GGNCC 518 521 . . 524 527 + CviJI RGCY 525 525 . . 527 531 - BsrI ACTGG 527 525 . . 526 531 - BfiI ACTGGG 521 520 . . 538 542 + BmgT120I GGNCC 539 540 . . 538 542 + AsuI* GGNCC 538 541 . . 539 542 + CviJI RGCY 540 540 . . 539 542 + HaeIII GGCC 540 540 . . 552 556 + BmgT120I GGNCC 553 554 . . 552 556 + AsuI* GGNCC 552 555 . . 553 556 + CviJI RGCY 554 554 . . 553 556 + HaeIII GGCC 554 554 . . 570 575 + Hpy178III* TCNNGA 571 573 . . 580 583 + AciI CCGC 580 582 . . 589 592 + MseI TTAA 589 591 . . 593 596 + CviJI RGCY 594 594 . . 614 619 + BclI TGATCA 614 618 . . 615 618 + DpnI GATC 616 616 . . 615 618 + BstKTI GATC 617 615 . . 615 618 + MboI GATC 614 618 . . 631 635 - Hin4II* CCTTC 625 624 . . 635 640 + BseRI GAGGAG 650 648 . . 635 638 - MnlI CCTC 628 627 . . 640 644 - BccI CCATC 634 635 . . 648 653 + NlaIV GGNNCC 650 650 . . 646 649 - AciI CCGC 646 648 . . 644 649 - BsrBI CCGCTC 646 646 . . 659 664 + Hpy178III* TCNNGA 660 662 . . 663 667 - Hin4II* CCTTC 657 656 . . 667 672 + ApoI RAATTY 667 671 . . 668 671 + TspEI AATT 667 671 . . 677 680 + MseI TTAA 677 679 . . 689 694 + Hpy178III* TCNNGA 690 692 . . 693 697 + MboII GAAGA 705 704 . . 693 705 + BarI GAAGNNNNNNTAC 685 680 717 712 697 702 + AclI AACGTT 698 700 . . 698 701 + TaiI ACGT 701 697 . . 698 701 + SetI ASST 701 697 . . 698 701 + MaeII ACGT 698 700 . . 700 705 + Hpy166II GTNNAC 702 702 . . 706 710 + TfiI GAWTC 706 709 . . 706 710 + HinfI GANTC 706 709 . . 711 714 + CviRI* TGCA 712 712 . . 710 715 - BsgI GTGCAG 695 693 . . 725 730 + EcoT22I ATGCAT 729 725 . . 726 729 + CviRI* TGCA 727 727 . . 752 756 + BssKI CCNGG 751 756 . . 752 756 + BseBI CCWGG 753 754 . . 752 756 + ScrFI CCNGG 753 754 . . 752 756 + EcoRII CCWGG 751 756 . . 750 755 - Eco57MI CTGRAG 735 733 . . 750 755 - GsuI CTGGAG 735 733 . . 759 763 + TfiI GAWTC 759 762 . . 759 763 + HinfI GANTC 759 762 . . 769 774 + Hpy166II GTNNAC 771 771 . . 779 789 + MwoI GCNNNNNNNGC 785 782 . . 784 789 + NspI RCATGY 788 784 . . 785 788 + FatI CATG 784 788 . . 785 788 + NlaIII CATG 788 784 . . 785 788 + CviAII CATG 785 787 . . 791 795 + MaeIII GTNAC 790 795 . . 788 791 - AciI CCGC 788 790 . . 796 800 + PleI GAGTC 804 805 . . 796 800 + MlyI GAGTC 805 805 . . 796 800 + HinfI GANTC 796 799 . . 810 813 + Hin6I GCGC 810 812 . . 810 813 + GlaI GCGC 811 811 . . 810 813 + HhaI GCGC 812 810 . . 820 823 + TspEI AATT 819 823 . . 825 830 + Cac8I GCNNGC 827 827 . . 827 832 + Hpy166II GTNNAC 829 829 . . 827 832 + SduI GDGCHC 831 827 . . 827 832 + BseSI GKGCMC 831 827 . . 827 832 + ApaLI GTGCAC 827 831 . . 827 832 + HgiAI* GWGCWC 831 827 . . 828 831 + CviRI* TGCA 829 829 . . 833 837 + BslFI GGGAC 847 851 . . 842 845 + SetI ASST 845 841 . . 842 852 + BsaXI ACNNNNNCTCC 832 829 862 859 846 851 + SduI GDGCHC 850 846 . . 846 851 + HgiAI* GWGCWC 850 846 . . 865 868 - MnlI CCTC 858 857 . . 871 880 + MslI CAYNNNNRTG 875 875 . . 877 882 + NlaIV GGNNCC 879 879 . . 877 882 + HgiCI* GGYRCC 877 881 . . 895 899 + Hpy188I TCNGA 897 896 . . 892 896 - MboII GAAGA 884 883 . . 893 898 - Eco57MI CTGRAG 878 876 . . 893 898 - Eco57I CTGAAG 878 876 . . 901 905 - TspDTI ATGAA 891 889 . . 905 909 + Hpy188I TCNGA 907 906 . . 920 924 + TspGWI ACGGA 935 933 . . 932 935 + MseI TTAA 932 934 . . 941 945 + BsrI ACTGG 946 944 . . 946 950 + Hpy188I TCNGA 948 947 . . 951 955 + BssKI CCNGG 950 955 . . 951 955 + BseBI CCWGG 952 953 . . 951 955 + ScrFI CCNGG 952 953 . . 951 955 + EcoRII CCWGG 950 955 . . 960 970 + MwoI GCNNNNNNNGC 966 963 . . 957 961 - SfaNI GCATC 947 951 . . 970 975 + Eco57MI CTGRAG 991 989 . . 970 975 + GsuI CTGGAG 991 989 . . 973 976 - MnlI CCTC 966 965 . . 989 993 + TspDTI ATGAA 1004 1002 . . 991 995 + MboII GAAGA 1003 1002 . . 991 996 + BbvII* GAAGAC 998 1002 . . 997 1003 + TspRI CASTGNN 1003 994 . . 996 1000 - BsrI ACTGG 996 994 . . 1005 1009 + MboII GAAGA 1017 1016 . . 1017 1028 + BstXI CCANNNNNNTGG 1024 1020 . . 1018 1027 + MslI CAYNNNNRTG 1022 1022 . . 1018 1023 + BsaAI YACGTR 1020 1020 . . 1018 1027 + OliI CACNNNNGTG 1022 1022 . . 1018 1023 + PmaCI CACGTG 1020 1020 . . 1019 1022 + TaiI ACGT 1022 1018 . . 1019 1022 + SetI ASST 1022 1018 . . 1019 1022 + MaeII ACGT 1019 1021 . . 1022 1027 - TaqII CACCCA 1012 1010 . . 1027 1032 + SduI GDGCHC 1031 1027 . . 1027 1032 + BseSI GKGCMC 1031 1027 . . 1030 1039 + MslI CAYNNNNRTG 1034 1034 . . 1030 1039 + OliI CACNNNNGTG 1034 1034 . . 1042 1046 + MboII GAAGA 1054 1053 . . 1042 1047 + BbvII* GAAGAC 1049 1053 . . 1048 1051 + CviRI* TGCA 1049 1049 . . 1057 1062 + ApoI RAATTY 1057 1061 . . 1058 1061 + TspEI AATT 1057 1061 . . 1062 1068 + Bpu10I CCTNAGC 1063 1066 . . 1063 1067 + DdeI CTNAG 1063 1066 . . 1065 1070 + HindIII AAGCTT 1065 1069 . . 1066 1069 + SetI ASST 1069 1065 . . 1066 1069 + CviJI RGCY 1067 1067 . . 1066 1069 + AluI AGCT 1067 1067 . . 1073 1078 + McrI* CGRYCG 1076 1074 . . 1073 1078 + PvuI CGATCG 1076 1074 . . 1074 1077 + DpnI GATC 1075 1075 . . 1074 1077 + BstKTI GATC 1076 1074 . . 1074 1077 + MboI GATC 1073 1077 . . 1076 1079 + TaqI TCGA 1076 1078 . . 1078 1081 - MnlI CCTC 1071 1070 . . 1091 1095 + Hpy188I TCNGA 1093 1092 . . 1089 1093 - FokI GGATG 1075 1079 . . 1089 1093 - BseGI GGATG 1088 1086 . . 1130 1140 + MwoI GCNNNNNNNGC 1136 1133 . . 1135 1138 + CviJI RGCY 1136 1136 . . 1136 1140 + TauI GCSGC 1139 1136 . . 1136 1140 + BlsI GCNGC 1138 1137 . . 1136 1140 + BisI GCNGC 1137 1138 . . 1137 1140 + AciI CCGC 1137 1139 . . 1138 1141 + FnuDII* CGCG 1139 1139 . . 1139 1144 + BtgZI GCGATG 1154 1158 . . 1141 1145 - BccI CCATC 1135 1136 . . 1153 1158 + BetI* WCCGGW 1153 1157 . . 1154 1157 + HpaII CCGG 1154 1156 . . 1158 1163 + AsuII TTCGAA 1159 1161 . . 1159 1162 + TaqI TCGA 1159 1161 . . 1171 1176 + BsmI GAATGC 1177 1175 . . 1167 1171 - BccI CCATC 1161 1162 . . 1183 1187 - SfaNI GCATC 1173 1177 . . 1190 1195 + Hpy178III* TCNNGA 1191 1193 . . 1194 1198 + HgaI GACGC 1203 1208 . . 1202 1205 + CviRI* TGCA 1203 1203 . . 1209 1213 + Hpy99I CGWCG 1213 1208 . . 1217 1220 + TspEI AATT 1216 1220 . . 1226 1229 + SetI ASST 1229 1225 . . 1227 1232 + Eco31I GGTCTC 1233 1237 . . 1228 1232 + BsmAI GTCTC 1233 1237 . . 1231 1235 + Hpy188I TCNGA 1233 1232 . . 1240 1243 + TaqI TCGA 1240 1242 . . 1240 1245 + Hpy178III* TCNNGA 1241 1243 . . 1247 1252 + XhoII RGATCY 1247 1251 . . 1247 1252 + BglII AGATCT 1247 1251 . . 1248 1251 + DpnI GATC 1249 1249 . . 1248 1251 + BstKTI GATC 1250 1248 . . 1248 1251 + MboI GATC 1247 1251 . . 1252 1256 + Hpy188I TCNGA 1254 1253 . . 1259 1264 + TatI WGTACW 1259 1263 . . 1260 1263 + Csp6I GTAC 1260 1262 . . 1260 1263 + RsaI GTAC 1261 1261 . . 1267 1271 + MboII GAAGA 1279 1278 . . 1273 1276 + CviJI RGCY 1274 1274 . . 1301 1304 + SetI ASST 1304 1300 . . 1301 1304 + CviJI RGCY 1302 1302 . . 1301 1304 + AluI AGCT 1302 1302 . . 1307 1312 + DsaI* CCRYGG 1307 1311 . . 1307 1312 + StyI CCWWGG 1307 1311 . . 1307 1312 + NcoI CCATGG 1307 1311 . . 1307 1312 + SecI* CCNNGG 1307 1311 . . 1308 1311 + FatI CATG 1307 1311 . . 1308 1311 + NlaIII CATG 1311 1307 . . 1308 1311 + CviAII CATG 1308 1310 . . 1316 1319 + TspEI AATT 1315 1319 . . 1329 1334 + Hpy166II GTNNAC 1331 1331 . . 1337 1340 + SetI ASST 1340 1336 . . 1337 1340 + CviJI RGCY 1338 1338 . . 1337 1340 + AluI AGCT 1338 1338 . . 1345 1348 + DpnI GATC 1346 1346 . . 1345 1348 + BstKTI GATC 1347 1345 . . 1345 1348 + MboI GATC 1344 1348 . . 1347 1352 + XbaI TCTAGA 1347 1351 . . 1347 1352 + Hpy178III* TCNNGA 1348 1350 . . 1348 1351 + MaeI CTAG 1348 1350 . . 1359 1363 + BsrI ACTGG 1364 1362 . . 1360 1365 + Eco57MI CTGRAG 1381 1379 . . 1360 1365 + GsuI CTGGAG 1381 1379 . . 1365 1369 - BccI CCATC 1359 1360 . . 1390 1394 + TfiI GAWTC 1390 1393 . . 1386 1390 - Hin4II* CCTTC 1380 1379 . . 1390 1394 + HinfI GANTC 1390 1393 . . 1393 1397 + Hpy188I TCNGA 1395 1394 . . 1401 1405 - SfaNI GCATC 1391 1395 . . 1410 1415 + McrI* CGRYCG 1413 1411 . . 1411 1416 + TaqII GACCGA 1427 1425 . . 1411 1421 + Hin4I GAYNNNNNVTC 1402 1397 1434 1429 1421 1427 + TspRI CASTGNN 1427 1418 . . 1411 1421 - Hin4I GAYNNNNNVTC 1434 1429 1402 1397 1423 1427 + DdeI CTNAG 1423 1426 . . 1427 1432 + Hpy166II GTNNAC 1429 1429 . . 1423 1427 - BspCNI CTCAG 1415 1413 . . 1423 1427 - BseMII CTCAG 1414 1412 . . 1434 1438 + TfiI GAWTC 1434 1437 . . 1434 1438 + HinfI GANTC 1434 1437 . . 1437 1442 + Hpy178III* TCNNGA 1438 1440 . . 1442 1445 + SetI ASST 1445 1441 . . 1456 1461 + Eco57MI CTGRAG 1477 1475 . . 1456 1461 + GsuI CTGGAG 1477 1475 . . 1459 1464 + BseRI GAGGAG 1474 1472 . . 1459 1462 - MnlI CCTC 1452 1451 . . 1474 1477 + TaqI TCGA 1474 1476 . . 1476 1480 + MboII GAAGA 1488 1487 . . 1482 1486 + BsrI ACTGG 1487 1485 . . 1486 1489 + Csp6I GTAC 1486 1488 . . 1486 1489 + RsaI GTAC 1487 1487 . . 1491 1494 + DpnI GATC 1492 1492 . . 1491 1494 + BstKTI GATC 1493 1491 . . 1491 1494 + MboI GATC 1490 1494 . . 1495 1499 + Hpy188I TCNGA 1497 1496 . . 1504 1509 + Hpy166II GTNNAC 1506 1506 . . 1504 1509 + AccI GTMKAC 1505 1507 . . 1507 1511 + HinfI GANTC 1507 1510 . . 1509 1514 + BmeT110I CYCGRG 1510 1512 . . 1509 1514 + AvaI CYCGRG 1509 1513 . . 1507 1511 - PleI GAGTC 1501 1502 . . 1507 1511 - MlyI GAGTC 1501 1501 . . 1521 1526 + SpeI ACTAGT 1521 1525 . . 1522 1525 + MaeI CTAG 1522 1524 . . 1533 1537 + BmgT120I GGNCC 1534 1535 . . 1533 1537 + AvaII GGWCC 1533 1536 . . 1533 1537 + AsuI* GGNCC 1533 1536 . . 1530 1534 - Hin4II* CCTTC 1524 1523 . . 1536 1540 + BssKI CCNGG 1535 1540 . . 1536 1540 + BseBI CCWGG 1537 1538 . . 1536 1540 + ScrFI CCNGG 1537 1538 . . 1536 1540 + EcoRII CCWGG 1535 1540 . . 1540 1543 - MnlI CCTC 1533 1532 . . 1544 1549 + BtgZI GCGATG 1559 1563 . . 1552 1556 + MboII GAAGA 1564 1563 . . 1552 1557 + BbvII* GAAGAC 1559 1563 . . 1556 1560 + BceAI ACGGC 1572 1574 . . 1564 1568 + BceAI ACGGC 1580 1582 . . 1565 1570 + CfrI YGGCCR 1565 1569 . . 1566 1569 + CviJI RGCY 1567 1567 . . 1566 1569 + HaeIII GGCC 1567 1567 . . 1576 1579 + FatI CATG 1575 1579 . . 1576 1579 + NlaIII CATG 1579 1575 . . 1576 1579 + CviAII CATG 1576 1578 . . 1573 1584 - TstI CACNNNNNNTCC 1597 1592 1565 1560 1587 1590 + TaiI ACGT 1590 1586 . . 1587 1590 + SetI ASST 1590 1586 . . 1587 1590 + MaeII ACGT 1587 1589 . . 1587 1592 + AflIII ACRYGT 1587 1591 . . 1595 1605 + MwoI GCNNNNNNNGC 1601 1598 . . 1600 1604 - Hin4II* CCTTC 1594 1593 . . 1606 1609 - MnlI CCTC 1599 1598 . . 1618 1622 - Hin4II* CCTTC 1612 1611 . . 1623 1634 + BcgI CGANNNNNNTGC 1612 1610 1646 1644 1624 1628 + HinfI GANTC 1624 1627 . . 1624 1628 - PleI GAGTC 1618 1619 . . 1624 1628 - MlyI GAGTC 1618 1618 . . 1632 1635 + CviRI* TGCA 1633 1633 . . 1629 1634 - BsrDI GCAATG 1628 1626 . . 1637 1640 + TspEI AATT 1636 1640 . . 1640 1643 + CviRI* TGCA 1641 1641 . . 1641 1645 + BlsI GCNGC 1643 1642 . . 1641 1645 + BisI GCNGC 1642 1643 . . 1641 1645 + BbvI GCAGC 1653 1657 . . 1641 1645 + TseI GCWGC 1641 1644 . . 1643 1646 + CviJI RGCY 1644 1644 . . 1646 1651 + SecI* CCNNGG 1646 1650 . . 1647 1651 + BssKI CCNGG 1646 1651 . . 1647 1651 + BseBI CCWGG 1648 1649 . . 1647 1651 + ScrFI CCNGG 1648 1649 . . 1647 1651 + EcoRII CCWGG 1646 1651 . . 1649 1652 + SetI ASST 1652 1648 . . 1651 1656 + Hpy166II GTNNAC 1653 1653 . . 1652 1657 + TatI WGTACW 1652 1656 . . 1652 1657 + Bsp1407I TGTACA 1652 1656 . . 1653 1656 + Csp6I GTAC 1653 1655 . . 1653 1656 + RsaI GTAC 1654 1654 . . 1659 1663 + Tsp4CI* ACNGT 1661 1660 . . 1660 1665 + DsaI* CCRYGG 1660 1664 . . 1660 1665 + SecI* CCNNGG 1660 1664 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 38 169 1467 # # AciI: # [CCGC] # 66 142 161 349 419 537 # # AclI: # [AACGTT] # 698 976 # # AflIII: # [ACRYGT] # 87 90 380 1117 # # AluI: # [AGCT] # 36 154 235 336 913 # # AlwNI: # [CAGNNNCTG] # 192 1482 # # ApaLI: # [GTGCAC] # 827 847 # # ApoI: # [RAATTY] # 301 366 390 617 # # AsuI*: # [GGNCC] # 14 20 141 204 314 981 # # AsuII: # [TTCGAA] # 274 515 885 # # AvaI: # [CYCGRG] # 165 1509 # # AvaII: # [GGWCC] # 141 518 1015 # # BarI: # [GAAGNNNNNNTAC] # 32 685 957 # # BbvI: # [GCAGC] # 21 1653 # # BbvII*: # [GAAGAC] # 51 115 510 998 # # BccI: # [CCATC] # 26 96 198 315 501 538 # # BceAI: # [ACGGC] # 8 94 381 1191 # # BcgI: # [CGANNNNNNTGC] # 28 34 1612 # # BclI: # [TGATCA] # 614 1060 # # BetI*: # [WCCGGW] # 521 1153 # # BfiI: # [ACTGGG] # 521 1153 # # BglII: # [AGATCT] # 427 1247 # # BinI*: # [GGATC] # 20 273 1381 # # BisI: # [GCNGC] # 32 505 1137 # # BlsI: # [GCNGC] # 31 505 1138 # # BmeT110I: # [CYCGRG] # 164 1510 # # BmgT120I: # [GGNCC] # 14 20 140 205 314 981 # # Bpu10I: # [CCTNAGC] # 611 1063 # # BsaAI: # [YACGTR] # 383 637 654 # # BsaXI: # [ACNNNNNCTCC] # 30 30 134 668 812 # # BseBI: # [CCWGG] # 26 111 199 217 237 299 # 585 # # BseGI: # [GGATG] # 30 420 586 638 # # BseMII: # [CTCAG] # 260 1414 # # BseRI: # [GAGGAG] # 73 138 200 439 824 # # BseSI: # [GKGCMC] # 200 643 831 # # BsgI: # [GTGCAG] # 695 979 # # BsiI*: # [CACGAG] # 283 1391 # # BslFI: # [GGGAC] # 13 827 834 # # BsmAI: # [GTCTC] # 441 1233 # # BsmI: # [GAATGC] # 497 1177 # # Bsp1407I: # [TGTACA] # 22 1652 # # BspCNI: # [CTCAG] # 259 1415 # # BsrBI: # [CCGCTC] # 646 1028 # # BsrDI: # [GCAATG] # 46 1628 # # BsrI: # [ACTGG] # 50 123 187 197 330 368 # 419 # # BssKI: # [CCNGG] # 18 28 111 199 215 237 # 281 585 # # BstKTI: # [GATC] # 9 11 97 146 174 181 # 281 316 459 # # BstXI: # [CCANNNNNNTGG] # 650 1024 # # BtgZI: # [GCGATG] # 115 405 1154 # # Cac8I: # [GCNNGC] # 827 847 # # CauII*: # [CCSGG] # 235 1439 # # CfrI: # [YGGCCR] # 109 1565 # # Csp6I: # [GTAC] # 15 21 167 226 384 861 # # CviAII: # [CATG] # 98 268 344 441 523 # # CviJI: # [RGCY] # 14 15 28 30 34 36 # 40 51 63 69 77 91 # 138 229 286 473 # # CviRI*: # [TGCA] # 8 15 33 102 154 220 # 430 712 # # DdeI: # [CTNAG] # 102 106 251 360 855 # # DpnI: # [GATC] # 9 11 97 146 174 182 # 280 316 459 # # DraII: # [RGGNCCY] # 204 1470 # # DraIII: # [CACNNNGTG] # 237 1437 # # DsaI*: # [CCRYGG] # 14 353 440 867 # # Eco31I: # [GGTCTC] # 441 1233 # # Eco57I: # [CTGAAG] # 796 878 # # Eco57MI: # [CTGRAG] # 96 113 143 197 214 390 # 521 # # EcoRII: # [CCWGG] # 28 111 199 215 237 299 # 585 # # EcoT22I: # [ATGCAT] # 729 945 # # FatI: # [CATG] # 99 268 344 440 523 # # FnuDII*: # [CGCG] # 472 535 667 # # FokI: # [GGATG] # 37 420 599 618 # # GlaI: # [GCGC] # 811 863 # # GsuI: # [CTGGAG] # 96 197 214 256 390 521 # # HaeIII: # [GGCC] # 14 107 205 335 1013 # # HgaI: # [GACGC] # 471 1203 # # HgiAI*: # [GWGCWC] # 19 824 831 # # HgiCI*: # [GGYRCC] # 797 877 # # HhaI: # [GCGC] # 812 862 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 240 436 # 934 # # Hin4II*: # [CCTTC] # 18 32 62 70 81 114 # 144 430 723 # # Hin6I: # [GCGC] # 810 864 # # HindIII: # [AAGCTT] # 609 1065 # # HinfI: # [GANTC] # 37 40 44 50 53 73 # 87 117 143 195 241 594 # # HpaII: # [CCGG] # 235 520 919 # # HphI: # [GGTGA] # 26 223 1425 # # Hpy166II: # [GTNNAC] # 21 39 58 69 77 98 # 147 502 663 # # Hpy178III*: # [TCNNGA] # 29 30 50 89 90 107 # 235 236 307 501 # # Hpy188I: # [TCNGA] # 10 21 41 102 140 141 # 145 177 391 506 # # Hpy99I: # [CGWCG] # 252 461 961 # # MaeI: # [CTAG] # 152 174 1348 # # MaeII: # [ACGT] # 87 316 321 382 568 # # MaeIII: # [GTNAC] # 790 884 # # MboI: # [GATC] # 9 11 97 146 174 184 # 278 316 459 # # MboII: # [GAAGA] # 14 34 37 76 110 119 # 152 179 209 225 231 288 # # McrI*: # [CGRYCG] # 56 261 337 1020 # # MluI: # [ACGCGT] # 470 1204 # # MlyI: # [GAGTC] # 34 56 117 696 771 # # MnlI: # [CCTC] # 10 51 56 66 72 75 # 78 81 105 108 166 195 # 230 381 # # MseI: # [TTAA] # 88 96 255 493 742 # # MslI: # [CAYNNNNRTG] # 12 147 640 875 # # MwoI: # [GCNNNNNNNGC] # 73 151 170 181 465 634 # # NcoI: # [CCATGG] # 367 440 867 # # NlaIII: # [CATG] # 95 268 344 444 523 # # NlaIV: # [GGNNCC] # 62 152 229 436 795 # # NspBII*: # [CMGCKG] # 421 1253 # # NspI: # [RCATGY] # 788 886 # # OliI: # [CACNNNNGTG] # 12 640 1022 # # PleI: # [GAGTC] # 34 56 117 697 770 # # PmaCI: # [CACGTG] # 654 1020 # # PvuI: # [CGATCG] # 598 1076 # # RsaI: # [GTAC] # 15 20 167 226 385 861 # # SauI*: # [CCTNAGG] # 208 1466 # # ScrFI: # [CCNGG] # 18 26 111 199 217 237 # 281 585 # # SduI: # [GDGCHC] # 19 181 643 831 # # SecI*: # [CCNNGG] # 14 14 56 74 339 384 # 793 # # SetI: # [ASST] # 22 36 47 51 58 62 # 75 105 120 144 145 156 # 160 177 316 # # SfaNI: # [GCATC] # 218 226 283 947 # # SpeI: # [ACTAGT] # 153 1521 # # SplI*: # [CGTACG] # 398 1276 # # SspI: # [AATATT] # 406 1268 # # StyI: # [CCWWGG] # 367 440 867 # # TaiI: # [ACGT] # 84 316 321 385 568 # # TaqI: # [TCGA] # 16 81 83 164 200 234 # 274 622 # # TaqII: # [GACCGA] # 11 70 247 415 931 # # TatI: # [WGTACW] # 22 393 1259 # # TauI: # [GCSGC] # 535 1139 # # TfiI: # [GAWTC] # 44 53 127 143 195 240 # 241 631 # # TseI: # [GCWGC] # 33 1641 # # Tsp4CI*: # [ACNGT] # 13 388 1273 # # TspDTI: # [ATGAA] # 113 314 577 670 # # TspEI: # [AATT] # 38 99 132 152 159 168 # 169 198 238 321 # # TspGWI: # [ACGGA] # 236 699 739 # # TspRI: # [CASTGNN] # 15 247 424 988 # # TstI: # [CACNNNNNNTCC] # 32 77 1565 # # XbaI: # [TCTAGA] # 327 1347 # # XhoII: # [RGATCY] # 20 278 427 949 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1674 # Reported_sequences: 1 # Reported_hitcount: 434 #---------------------------------------