Restriction Fragments for YFLWtau1



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YFLWtau1 on chromosome VI from coordinates 144203 to 144523.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.3920.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + MfeI CAATTG 6 10 . . 7 10 + TspEI AATT 6 10 . . 17 21 + MaeIII GTNAC 16 21 . . 27 32 + BsmI GAATGC 33 31 . . 31 36 + Cac8I GCNNGC 33 33 . . 34 37 + CviRI* TGCA 35 35 . . 35 39 + SfaNI GCATC 44 48 . . 41 44 + CviRI* TGCA 42 42 . . 43 46 + FatI CATG 42 46 . . 43 46 + NlaIII CATG 46 42 . . 43 46 + CviAII CATG 43 45 . . 44 48 + TspDTI ATGAA 59 57 . . 55 58 + AciI CCGC 55 57 . . 67 70 + MseI TTAA 67 69 . . 73 77 + TspDTI ATGAA 88 86 . . 81 84 + SetI ASST 84 80 . . 86 89 + MseI TTAA 86 88 . . 98 102 + TspDTI ATGAA 113 111 . . 100 104 + TfiI GAWTC 100 103 . . 100 104 + HinfI GANTC 100 103 . . 111 115 + MboII GAAGA 123 122 . . 116 120 + Tsp4CI* ACNGT 118 117 . . 121 124 + SetI ASST 124 120 . . 129 132 + FatI CATG 128 132 . . 129 132 + NlaIII CATG 132 128 . . 129 132 + CviAII CATG 129 131 . . 132 135 + DpnI GATC 133 133 . . 132 135 + BstKTI GATC 134 132 . . 132 135 + MboI GATC 131 135 . . 150 155 + MluI ACGCGT 150 154 . . 150 155 + AflIII ACRYGT 150 154 . . 151 154 + FnuDII* CGCG 152 152 . . 162 167 + SpeI ACTAGT 162 166 . . 163 166 + MaeI CTAG 163 165 . . 189 194 + Hpy178III* TCNNGA 190 192 . . 178 190 - PsrI GAACNNNNNNTAC 202 197 170 165 193 205 + PsrI GAACNNNNNNTAC 185 180 217 212 208 213 + BclI TGATCA 208 212 . . 209 212 + DpnI GATC 210 210 . . 209 212 + BstKTI GATC 211 209 . . 209 212 + MboI GATC 208 212 . . 268 272 + MboII GAAGA 280 279 . . 270 275 + XhoII RGATCY 270 274 . . 271 274 + DpnI GATC 272 272 . . 271 274 + BstKTI GATC 273 271 . . 271 274 + MboI GATC 270 274 . . 271 275 - BinI* GGATC 265 266 . . 280 283 + MseI TTAA 280 282 . . 284 288 - MboII GAAGA 276 275 . . 286 290 - TspDTI ATGAA 276 274 . . 290 293 + MseI TTAA 290 292 . . 303 306 + MseI TTAA 303 305 . . 306 309 + SetI ASST 309 305 . . 307 310 + MnlI CCTC 317 316 . . 312 315 + TspEI AATT 311 315 . . #--------------------------------------- # # # AciI: # [CCGC] # 55 266 # # AflIII: # [ACRYGT] # 150 171 # # BclI: # [TGATCA] # 113 208 # # BinI*: # [GGATC] # 56 265 # # BsmI: # [GAATGC] # 33 288 # # BstKTI: # [GATC] # 48 62 77 134 # # Cac8I: # [GCNNGC] # 33 288 # # CviAII: # [CATG] # 43 86 192 # # CviRI*: # [TGCA] # 7 35 279 # # DpnI: # [GATC] # 49 62 77 133 # # FatI: # [CATG] # 42 86 193 # # FnuDII*: # [CGCG] # 152 169 # # HinfI: # [GANTC] # 100 221 # # Hpy178III*: # [TCNNGA] # 131 190 # # MaeI: # [CTAG] # 158 163 # # MaeIII: # [GTNAC] # 16 305 # # MboI: # [GATC] # 51 62 77 131 # # MboII: # [GAAGA] # 4 41 123 153 # # MfeI: # [CAATTG] # 6 315 # # MluI: # [ACGCGT] # 150 171 # # MnlI: # [CCTC] # 4 317 # # MseI: # [TTAA] # 10 13 18 19 67 194 # # NlaIII: # [CATG] # 46 86 189 # # PsrI: # [GAACNNNNNNTAC] # 15 15 17 104 170 # # SetI: # [ASST] # 12 40 84 185 # # SfaNI: # [GCATC] # 44 277 # # SpeI: # [ACTAGT] # 159 162 # # TfiI: # [GAWTC] # 100 221 # # Tsp4CI*: # [ACNGT] # 118 203 # # TspDTI: # [ATGAA] # 25 29 45 59 163 # # TspEI: # [AATT] # 6 10 305 # # XhoII: # [RGATCY] # 51 270 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 321 # Reported_sequences: 1 # Reported_hitcount: 55 #---------------------------------------