Restriction Fragments for YFLWdelta2



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YFLWdelta2 on chromosome VI from coordinates 137663 to 137912.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.14944.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 58 62 + Tsp4CI* ACNGT 60 59 . . 59 65 + TspRI CASTGNN 65 56 . . 89 92 + SetI ASST 92 88 . . 89 92 + CviJI RGCY 90 90 . . 89 92 + AluI AGCT 90 90 . . 85 95 - Hin4I GAYNNNNNVTC 108 103 76 71 97 100 + TaqI TCGA 97 99 . . 106 110 + MboII GAAGA 118 117 . . 111 114 + SetI ASST 114 110 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + AluI AGCT 112 112 . . 119 122 + CviRI* TGCA 120 120 . . 146 150 + TspDTI ATGAA 161 159 . . 160 164 + TspGWI ACGGA 175 173 . . 174 183 + MslI CAYNNNNRTG 178 178 . . 194 198 + TfiI GAWTC 194 197 . . 194 198 + HinfI GANTC 194 197 . . 207 212 + EcoRI GAATTC 207 211 . . 207 212 + ApoI RAATTY 207 211 . . 208 211 + TspEI AATT 207 211 . . 219 226 + PspXI VCTCGAGB 220 224 . . 219 222 + MnlI CCTC 229 228 . . 219 226 + AbsI CCTCGAGG 220 224 . . 220 225 + SmlI CTYRAG 220 224 . . 220 225 + XhoI CTCGAG 220 224 . . 220 225 + BmeT110I CYCGRG 221 223 . . 220 225 + AvaI CYCGRG 220 224 . . 221 224 + TaqI TCGA 221 223 . . 223 228 + BseRI GAGGAG 238 236 . . 223 226 - MnlI CCTC 216 215 . . 234 237 + MaeI CTAG 234 236 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 30 220 # # AluI: # [AGCT] # 22 90 138 # # ApoI: # [RAATTY] # 43 207 # # AvaI: # [CYCGRG] # 30 220 # # BmeT110I: # [CYCGRG] # 29 221 # # BseRI: # [GAGGAG] # 12 238 # # CviJI: # [RGCY] # 22 90 138 # # CviRI*: # [TGCA] # 120 130 # # EcoRI: # [GAATTC] # 43 207 # # Hin4I: # [GAYNNNNNVTC] # 32 76 142 # # HinfI: # [GANTC] # 56 194 # # MaeI: # [CTAG] # 16 234 # # MboII: # [GAAGA] # 118 132 # # MnlI: # [CCTC] # 13 21 216 # # MslI: # [CAYNNNNRTG] # 72 178 # # PspXI: # [VCTCGAGB] # 30 220 # # SetI: # [ASST] # 22 92 136 # # SmlI: # [CTYRAG] # 30 220 # # TaqI: # [TCGA] # 29 97 124 # # TfiI: # [GAWTC] # 56 194 # # Tsp4CI*: # [ACNGT] # 60 190 # # TspDTI: # [ATGAA] # 89 161 # # TspEI: # [AATT] # 43 207 # # TspGWI: # [ACGGA] # 75 175 # # TspRI: # [CASTGNN] # 65 185 # # XhoI: # [CTCGAG] # 30 220 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 250 # Reported_sequences: 1 # Reported_hitcount: 31 #---------------------------------------