Restriction Fragments for THI5/YFL058W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

THI5/YFL058W on chromosome VI from coordinates 12929 to 13951.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.21698.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 3 8 + Hpy166II GTNNAC 5 5 . . 3 8 + AccI GTMKAC 4 6 . . 5 10 + SfeI* CTRYAG 5 9 . . 15 18 + DpnI GATC 16 16 . . 15 18 + BstKTI GATC 17 15 . . 15 18 + MboI GATC 14 18 . . 32 36 + BsrI ACTGG 37 35 . . 60 63 + TspEI AATT 59 63 . . 69 72 + CviJI RGCY 70 70 . . 81 84 + SetI ASST 84 80 . . 83 87 + MaeIII GTNAC 82 87 . . 77 90 - AjuI GAANNNNNNNTTGG 102 97 70 65 99 102 + SetI ASST 102 98 . . 102 107 + XbaI TCTAGA 102 106 . . 102 107 + Hpy178III* TCNNGA 103 105 . . 103 106 + MaeI CTAG 103 105 . . 108 111 + FatI CATG 107 111 . . 108 111 + NlaIII CATG 111 107 . . 108 111 + CviAII CATG 108 110 . . 110 115 + BalI TGGCCA 112 112 . . 110 115 + CfrI YGGCCR 110 114 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + HaeIII GGCC 112 112 . . 113 117 + BccI CCATC 121 122 . . 114 118 - FokI GGATG 100 104 . . 114 118 - BseGI GGATG 113 111 . . 118 121 + MaeI CTAG 118 120 . . 133 137 + Hin4II* CCTTC 143 142 . . 136 140 + Hpy188I TCNGA 138 137 . . 124 137 - AjuI GAANNNNNNNTTGG 149 144 117 112 142 146 + Tsp45I GTSAC 141 146 . . 142 146 + MaeIII GTNAC 141 146 . . 144 150 + TspRI CASTGNN 150 141 . . 146 150 + DdeI CTNAG 146 149 . . 146 150 - BspCNI CTCAG 138 136 . . 146 150 - BseMII CTCAG 137 135 . . 151 154 + MseI TTAA 151 153 . . 153 156 + TspEI AATT 152 156 . . 157 162 + XhoII RGATCY 157 161 . . 157 161 + BinI* GGATC 165 166 . . 158 161 + DpnI GATC 159 159 . . 158 161 + BstKTI GATC 160 158 . . 158 161 + MboI GATC 157 161 . . 167 170 + SetI ASST 170 166 . . 169 174 + SalI GTCGAC 169 173 . . 169 174 + Hpy166II GTNNAC 171 171 . . 169 174 + HindII GTYRAC 171 171 . . 169 174 + AccI GTMKAC 170 172 . . 170 173 + TaqI TCGA 170 172 . . 174 177 + FatI CATG 173 177 . . 174 177 + NlaIII CATG 177 173 . . 174 177 + CviAII CATG 174 176 . . 186 189 + CviJI RGCY 187 187 . . 189 192 + FatI CATG 188 192 . . 189 192 + NlaIII CATG 192 188 . . 189 192 + CviAII CATG 189 191 . . 192 195 + DpnI GATC 193 193 . . 192 195 + BstKTI GATC 194 192 . . 192 195 + MboI GATC 191 195 . . 195 205 + BsiYI* CCNNNNNNNGG 201 198 . . 195 205 + PflMI CCANNNNNTGG 201 198 . . 192 196 - BinI* GGATC 186 187 . . 199 202 + SetI ASST 202 198 . . 200 205 + StyI CCWWGG 200 204 . . 200 205 + SecI* CCNNGG 200 204 . . 204 207 + CviJI RGCY 205 205 . . 205 215 + MwoI GCNNNNNNNGC 211 208 . . 205 209 + BlsI GCNGC 207 206 . . 205 209 + BisI GCNGC 206 207 . . 205 209 + TseI GCWGC 205 208 . . 209 214 + StyI CCWWGG 209 213 . . 209 214 + SecI* CCNNGG 209 213 . . 205 209 - BbvI GCAGC 192 196 . . 210 221 + CspCI CAANNNNNGTGG 198 196 233 231 213 216 + CviJI RGCY 214 214 . . 213 216 + HaeIII GGCC 214 214 . . 213 217 + BmgT120I GGNCC 214 215 . . 213 217 + AsuI* GGNCC 213 216 . . 216 221 + DsaI* CCRYGG 216 220 . . 216 221 + SecI* CCNNGG 216 220 . . 227 233 + TspRI CASTGNN 233 224 . . 229 233 + Tsp45I GTSAC 228 233 . . 229 233 + MaeIII GTNAC 228 233 . . 226 230 - BsrI ACTGG 226 224 . . 225 230 - BfiI ACTGGG 220 219 . . 232 235 + SetI ASST 235 231 . . 233 236 + MnlI CCTC 243 242 . . 242 245 + MnlI CCTC 252 251 . . 249 254 - MmeI TCCRAC 230 228 . . 259 269 + BsiYI* CCNNNNNNNGG 265 262 . . 265 270 + Cfr10I RCCGGY 265 269 . . 265 270 + BetI* WCCGGW 265 269 . . 265 270 + AgeI ACCGGT 265 269 . . 266 269 + HpaII CCGG 266 268 . . 275 280 + TatI WGTACW 275 279 . . 276 279 + Csp6I GTAC 276 278 . . 276 279 + RsaI GTAC 277 277 . . 280 283 + MseI TTAA 280 282 . . 287 292 + BtsI GCAGTG 294 292 . . 288 294 + TspRI CASTGNN 294 285 . . 297 303 + TspRI CASTGNN 303 294 . . 299 304 + Eco57MI CTGRAG 320 318 . . 299 304 + Eco57I CTGAAG 320 318 . . 301 305 + MboII GAAGA 313 312 . . 301 306 + BbvII* GAAGAC 308 312 . . 304 314 + Hin4I GAYNNNNNVTC 295 290 327 322 304 314 + Eam1105I GACNNNNNGTC 309 308 . . 309 313 - BsrI ACTGG 309 307 . . 304 314 - Hin4I GAYNNNNNVTC 327 322 295 290 312 316 - BslFI GGGAC 297 301 . . 327 331 + MboII GAAGA 339 338 . . 327 339 + BarI GAAGNNNNNNTAC 319 314 351 346 330 333 + DpnI GATC 331 331 . . 330 333 + BstKTI GATC 332 330 . . 330 333 + MboI GATC 329 333 . . 335 339 + MaeIII GTNAC 334 339 . . 338 341 + TaiI ACGT 341 337 . . 338 341 + SetI ASST 341 337 . . 338 341 + MaeII ACGT 338 340 . . 343 347 + HphI GGTGA 355 354 . . 346 351 + EcoRI GAATTC 346 350 . . 346 351 + ApoI RAATTY 346 350 . . 347 350 + TspEI AATT 346 350 . . 356 361 + XhoII RGATCY 356 360 . . 357 360 + DpnI GATC 358 358 . . 357 360 + BstKTI GATC 359 357 . . 357 366 + BsaBI GATNNNNATC 361 361 . . 357 360 + MboI GATC 356 360 . . 357 361 - BinI* GGATC 351 352 . . 364 369 + ClaI ATCGAT 365 367 . . 365 368 + TaqI TCGA 365 367 . . 368 372 + TspDTI ATGAA 383 381 . . 371 374 + TspEI AATT 370 374 . . 386 390 + Tsp4CI* ACNGT 388 387 . . 391 395 + TspDTI ATGAA 406 404 . . 395 398 + CviJI RGCY 396 396 . . 400 404 + MboII GAAGA 412 411 . . 400 405 + BbvII* GAAGAC 407 411 . . 410 413 + AciI CCGC 410 412 . . 416 420 + Hpy188I TCNGA 418 417 . . 412 416 - BceAI ACGGC 397 399 . . 427 431 + TspDTI ATGAA 442 440 . . 440 445 + TatI WGTACW 440 444 . . 441 444 + Csp6I GTAC 441 443 . . 441 444 + RsaI GTAC 442 442 . . 449 452 + TaqI TCGA 449 451 . . 453 456 + SetI ASST 456 452 . . 451 455 - Hin4II* CCTTC 445 444 . . 466 471 + Cfr10I RCCGGY 466 470 . . 463 467 - SfaNI GCATC 453 457 . . 467 470 + HpaII CCGG 467 469 . . 479 482 + TaqI TCGA 479 481 . . 488 491 + CviRI* TGCA 489 489 . . 497 500 + TaqI TCGA 497 499 . . 500 503 + TspEI AATT 499 503 . . 505 509 + MboII GAAGA 517 516 . . 509 514 + TatI WGTACW 509 513 . . 509 514 + ScaI AGTACT 511 511 . . 510 513 + Csp6I GTAC 510 512 . . 505 510 - Ksp632I* CTCTTC 500 503 . . 510 513 + RsaI GTAC 511 511 . . 515 520 + BalI TGGCCA 517 517 . . 515 520 + CfrI YGGCCR 515 519 . . 516 519 + CviJI RGCY 517 517 . . 516 519 + HaeIII GGCC 517 517 . . 533 541 + AlwNI CAGNNNCTG 538 535 . . 534 537 + SetI ASST 537 533 . . 534 537 + CviJI RGCY 535 535 . . 534 537 + AluI AGCT 535 535 . . 535 545 + MwoI GCNNNNNNNGC 541 538 . . 538 542 + Hpy188I TCNGA 540 539 . . 541 545 - SfaNI GCATC 531 535 . . 558 561 + TspEI AATT 557 561 . . 570 573 + CviJI RGCY 571 571 . . 571 576 + Cac8I GCNNGC 573 573 . . 575 585 + MwoI GCNNNNNNNGC 581 578 . . 596 599 + Csp6I GTAC 596 598 . . 596 599 + RsaI GTAC 597 597 . . 598 602 + Tsp4CI* ACNGT 600 599 . . 613 616 + CviRI* TGCA 614 614 . . 620 624 + TspDTI ATGAA 635 633 . . 622 627 + ApoI RAATTY 622 626 . . 623 626 + TspEI AATT 622 626 . . 630 634 + MboII GAAGA 642 641 . . 633 637 + MboII GAAGA 645 644 . . 639 649 + BsiYI* CCNNNNNNNGG 645 642 . . 647 650 + SetI ASST 650 646 . . 650 654 + Hpy188I TCNGA 652 651 . . 659 664 + Hpy178III* TCNNGA 660 662 . . 662 673 + BdaI TGANNNNNNTCA 651 649 685 683 666 669 + CviJI RGCY 667 667 . . 668 672 + BccI CCATC 676 677 . . 671 676 + Hpy178III* TCNNGA 672 674 . . 662 673 - BdaI TGANNNNNNTCA 685 683 651 649 675 679 - Hin4II* CCTTC 669 668 . . 689 692 + TaiI ACGT 692 688 . . 689 692 + SetI ASST 692 688 . . 689 692 + MaeII ACGT 689 691 . . 694 697 + MaeI CTAG 694 696 . . 696 699 + CviJI RGCY 697 697 . . 702 712 + BsiYI* CCNNNNNNNGG 708 705 . . 711 714 + CviJI RGCY 712 712 . . 708 712 - Hin4II* CCTTC 702 701 . . 728 731 + TaqI TCGA 728 730 . . 737 740 + CviJI RGCY 738 738 . . 739 742 + MnlI CCTC 749 748 . . 742 747 + MfeI CAATTG 742 746 . . 743 746 + TspEI AATT 742 746 . . 754 757 + DpnI GATC 755 755 . . 754 757 + BstKTI GATC 756 754 . . 754 757 + MboI GATC 753 757 . . 782 786 + MaeIII GTNAC 781 786 . . 795 800 + Ksp632I* CTCTTC 801 804 . . 796 800 - MboII GAAGA 788 787 . . 798 802 - TspDTI ATGAA 788 786 . . 806 811 + TatI WGTACW 806 810 . . 806 811 + Bsp1407I TGTACA 806 810 . . 807 810 + Csp6I GTAC 807 809 . . 807 810 + RsaI GTAC 808 808 . . 814 819 + Hpy166II GTNNAC 816 816 . . 818 822 + Tsp4CI* ACNGT 820 819 . . 816 820 - HphI GGTGA 808 807 . . 821 825 + Tsp45I GTSAC 820 825 . . 821 825 + MaeIII GTNAC 820 825 . . 824 828 + BsrI ACTGG 829 827 . . 828 832 + MboII GAAGA 840 839 . . 833 836 + SetI ASST 836 832 . . 834 840 + BstEII GGTNACC 834 839 . . 835 839 + MaeIII GTNAC 834 839 . . 831 835 - Hin4II* CCTTC 825 824 . . 838 843 + Cfr10I RCCGGY 838 842 . . 838 843 + BetI* WCCGGW 838 842 . . 838 843 + AgeI ACCGGT 838 842 . . 839 842 + HpaII CCGG 839 841 . . 842 846 + MaeIII GTNAC 841 846 . . 845 849 + Tsp4CI* ACNGT 847 846 . . 854 864 + Hin4I GAYNNNNNVTC 845 840 877 872 857 862 + TsoI TARCCA 873 871 . . 858 861 + CviJI RGCY 859 859 . . 860 864 + BccI CCATC 868 869 . . 854 864 - Hin4I GAYNNNNNVTC 877 872 845 840 874 884 + Hin4I GAYNNNNNVTC 865 860 897 892 874 882 + Tth111I GACNNNGTC 877 878 . . 880 884 + BsmAI GTCTC 885 889 . . 881 886 + Hpy178III* TCNNGA 882 884 . . 883 886 + TaqI TCGA 883 885 . . 896 900 + TspDTI ATGAA 911 909 . . 908 912 + BssKI CCNGG 907 912 . . 908 912 + BseBI CCWGG 909 910 . . 908 912 + ScrFI CCNGG 909 910 . . 908 912 + EcoRII CCWGG 907 912 . . 910 915 + BalI TGGCCA 912 912 . . 910 915 + CfrI YGGCCR 910 914 . . 911 914 + CviJI RGCY 912 912 . . 911 914 + HaeIII GGCC 912 912 . . 922 926 + MboII GAAGA 934 933 . . 926 929 + SetI ASST 929 925 . . 922 927 - Ksp632I* CTCTTC 917 920 . . 925 928 - MnlI CCTC 918 917 . . 931 935 + Hpy188I TCNGA 933 932 . . 934 937 + DpnI GATC 935 935 . . 934 937 + BstKTI GATC 936 934 . . 934 937 + MboI GATC 933 937 . . 934 938 - BinI* GGATC 928 929 . . 945 948 + SetI ASST 948 944 . . 945 948 + CviJI RGCY 946 946 . . 945 948 + AluI AGCT 946 946 . . 960 963 + CviJI RGCY 961 961 . . 957 961 - BccI CCATC 951 952 . . 959 964 - TsoI TARCCA 949 947 . . 965 969 - TspDTI ATGAA 955 953 . . 969 974 + Hpy178III* TCNNGA 970 972 . . 979 982 + CviRI* TGCA 980 980 . . 990 993 + SetI ASST 993 989 . . 988 992 - Hin4II* CCTTC 982 981 . . 992 995 + Csp6I GTAC 992 994 . . 992 995 + RsaI GTAC 993 993 . . 998 1003 + Hpy178III* TCNNGA 999 1001 . . 1002 1012 + BplI GAGNNNNNCTC 993 988 . . 1008 1011 + CviJI RGCY 1009 1009 . . 1009 1019 + MwoI GCNNNNNNNGC 1015 1012 . . 1009 1015 + SapI GCTCTTC 1016 1019 . . 1010 1015 + Ksp632I* CTCTTC 1016 1019 . . 1011 1015 - MboII GAAGA 1003 1002 . . 1017 1020 + SetI ASST 1020 1016 . . 1017 1020 + CviJI RGCY 1018 1018 . . 1017 1020 + AluI AGCT 1018 1018 . . 1020 1023 + MseI TTAA 1020 1022 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 4 166 853 # # AciI: # [CCGC] # 410 613 # # AgeI: # [ACCGGT] # 185 265 573 # # AjuI: # [GAANNNNNNNTTGG] # 15 32 32 70 874 # # AluI: # [AGCT] # 5 72 411 535 # # AlwNI: # [CAGNNNCTG] # 485 538 # # ApoI: # [RAATTY] # 276 346 401 # # AsuI*: # [GGNCC] # 213 810 # # BalI: # [TGGCCA] # 111 112 395 405 # # BarI: # [GAAGNNNNNNTAC] # 32 319 672 # # BbvI: # [GCAGC] # 192 831 # # BbvII*: # [GAAGAC] # 99 308 616 # # BccI: # [CCATC] # 72 83 121 192 555 # # BceAI: # [ACGGC] # 397 626 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 338 651 # # BetI*: # [WCCGGW] # 185 265 573 # # BfiI: # [ACTGGG] # 220 803 # # BinI*: # [GGATC] # 21 95 165 165 577 # # BisI: # [GCNGC] # 206 817 # # BlsI: # [GCNGC] # 207 816 # # BmgT120I: # [GGNCC] # 214 809 # # BplI: # [GAGNNNNNCTC] # 30 993 # # BsaBI: # [GATNNNNATC] # 361 662 # # BseBI: # [CCWGG] # 114 909 # # BseGI: # [GGATG] # 113 910 # # BseMII: # [CTCAG] # 137 886 # # BsiYI*: # [CCNNNNNNNGG] # 63 64 201 315 380 # # BslFI: # [GGGAC] # 297 726 # # BsmAI: # [GTCTC] # 138 885 # # Bsp1407I: # [TGTACA] # 217 806 # # BspCNI: # [CTCAG] # 138 885 # # BsrI: # [ACTGG] # 37 83 189 194 520 # # BssKI: # [CCNGG] # 116 907 # # BstEII: # [GGTNACC] # 189 834 # # BstKTI: # [GATC] # 17 27 34 87 138 143 # 180 397 # # BtsI: # [GCAGTG] # 294 729 # # Cac8I: # [GCNNGC] # 450 573 # # Cfr10I: # [RCCGGY] # 185 201 265 372 # # CfrI: # [YGGCCR] # 110 113 395 405 # # ClaI: # [ATCGAT] # 365 658 # # Csp6I: # [GTAC] # 31 69 86 165 185 211 # 276 # # CspCI: # [CAANNNNNGTGG] # 35 198 790 # # CviAII: # [CATG] # 15 66 108 834 # # CviJI: # [RGCY] # 5 9 9 15 15 18 # 18 26 30 34 36 42 # 48 53 70 75 96 121 # 121 182 # # CviRI*: # [TGCA] # 43 125 366 489 # # DdeI: # [CTNAG] # 146 877 # # DpnI: # [GATC] # 16 27 34 88 138 143 # 180 397 # # DsaI*: # [CCRYGG] # 216 807 # # Eam1105I: # [GACNNNNNGTC] # 309 714 # # Eco57I: # [CTGAAG] # 320 703 # # Eco57MI: # [CTGRAG] # 320 703 # # EcoRI: # [GAATTC] # 346 677 # # EcoRII: # [CCWGG] # 116 907 # # FatI: # [CATG] # 15 66 107 835 # # FokI: # [GGATG] # 100 923 # # HaeIII: # [GGCC] # 102 111 112 303 395 # # Hin4I: # [GAYNNNNNVTC] # 0 0 0 0 12 20 # 20 32 126 295 518 # # Hin4II*: # [CCTTC] # 33 41 123 143 157 224 # 302 # # HindII: # [GTYRAC] # 171 852 # # HpaII: # [CCGG] # 184 201 266 372 # # HphI: # [GGTGA] # 215 355 453 # # Hpy166II: # [GTNNAC] # 5 166 207 645 # # Hpy178III*: # [TCNNGA] # 12 24 29 88 103 210 # 557 # # Hpy188I: # [TCNGA] # 90 112 122 138 280 281 # # Ksp632I*: # [CTCTTC] # 7 99 116 301 500 # # MaeI: # [CTAG] # 15 103 329 576 # # MaeII: # [ACGT] # 334 338 351 # # MaeIII: # [GTNAC] # 7 14 39 59 82 87 # 106 182 447 # # MboI: # [GATC] # 14 27 34 90 138 143 # 180 397 # # MboII: # [GAAGA] # 3 20 26 52 69 73 # 94 105 125 143 313 # # MfeI: # [CAATTG] # 281 742 # # MmeI: # [TCCRAC] # 230 793 # # MnlI: # [CCTC] # 9 105 169 243 497 # # MseI: # [TTAA] # 3 129 151 740 # # MwoI: # [GCNNNNNNNGC] # 8 40 211 330 434 # # NlaIII: # [CATG] # 15 66 111 831 # # PflMI: # [CCANNNNNTGG] # 201 822 # # RsaI: # [GTAC] # 30 69 86 165 185 211 # 277 # # SalI: # [GTCGAC] # 169 854 # # SapI: # [GCTCTTC] # 7 1016 # # ScaI: # [AGTACT] # 511 512 # # ScrFI: # [CCNGG] # 114 909 # # SecI*: # [CCNNGG] # 7 9 200 807 # # SetI: # [ASST] # 3 18 19 27 32 33 # 42 45 68 81 84 93 # 106 113 115 144 # # SfaNI: # [GCATC] # 78 453 492 # # SfeI*: # [CTRYAG] # 5 1018 # # StyI: # [CCWWGG] # 9 200 814 # # TaiI: # [ACGT] # 331 341 351 # # TaqI: # [TCGA] # 18 30 84 140 155 170 # 195 231 # # TatI: # [WGTACW] # 69 165 217 275 297 # # TseI: # [GCWGC] # 205 818 # # TsoI: # [TARCCA] # 74 76 873 # # Tsp45I: # [GTSAC] # 87 141 203 592 # # Tsp4CI*: # [ACNGT] # 27 176 212 220 388 # # TspDTI: # [ATGAA] # 23 36 44 68 123 153 # 193 383 # # TspEI: # [AATT] # 24 58 59 65 93 120 # 129 194 281 # # TspRI: # [CASTGNN] # 9 61 83 150 720 # # Tth111I: # [GACNNNGTC] # 146 877 # # XbaI: # [TCTAGA] # 102 921 # # XhoII: # [RGATCY] # 157 199 667 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1023 # Reported_sequences: 1 # Reported_hitcount: 288 #---------------------------------------