Restriction Fragments for YFL051C



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The currently selected gene/sequence is :

YFL051C on chromosome VI from coordinates 30540 to 30058.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.21725.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 20 - TspGWI ACGGA 6 4 . . 38 42 - MboII GAAGA 30 29 . . 37 42 - BbvII* GAAGAC 30 34 . . 45 50 + HaeII RGCGCY 49 45 . . 46 49 + Hin6I GCGC 46 48 . . 46 49 + GlaI GCGC 47 47 . . 46 49 + HhaI GCGC 48 46 . . 48 53 + Cac8I GCNNGC 50 50 . . 48 53 + BmtI GCTAGC 52 48 . . 48 53 + NheI GCTAGC 48 52 . . 49 52 + MaeI CTAG 49 51 . . 51 54 + SetI ASST 54 50 . . 51 54 + CviJI RGCY 52 52 . . 51 54 + AluI AGCT 52 52 . . 61 64 + SetI ASST 64 60 . . 62 68 + Bpu10I CCTNAGC 63 66 . . 63 67 + DdeI CTNAG 63 66 . . 65 70 + TsoI TARCCA 81 79 . . 66 69 + CviJI RGCY 67 67 . . 70 75 + TatI WGTACW 70 74 . . 71 74 + Csp6I GTAC 71 73 . . 71 74 + RsaI GTAC 72 72 . . 68 72 - BsrI ACTGG 68 66 . . 76 88 + BarI GAAGNNNNNNTAC 68 63 100 95 77 82 + HindIII AAGCTT 77 81 . . 78 81 + SetI ASST 81 77 . . 78 81 + CviJI RGCY 79 79 . . 78 81 + AluI AGCT 79 79 . . 79 84 + Cac8I GCNNGC 81 81 . . 103 107 + MboII GAAGA 115 114 . . 106 110 - BccI CCATC 100 101 . . 112 116 + TspDTI ATGAA 127 125 . . 115 120 + SspI AATATT 117 117 . . 118 123 + VspI ATTAAT 119 121 . . 119 122 + MseI TTAA 119 121 . . 121 124 + TspEI AATT 120 124 . . 131 136 + TatI WGTACW 131 135 . . 132 137 + Hpy166II GTNNAC 134 134 . . 132 135 + Csp6I GTAC 132 134 . . 132 135 + RsaI GTAC 133 133 . . 152 163 + BstXI CCANNNNNNTGG 159 155 . . 162 167 + NlaIV GGNNCC 164 164 . . 164 167 + CviJI RGCY 165 165 . . 178 181 + CviJI RGCY 179 179 . . 182 186 + TspDTI ATGAA 197 195 . . 207 210 + SetI ASST 210 206 . . 208 213 + NlaIV GGNNCC 210 210 . . 211 215 - TspGWI ACGGA 201 199 . . 218 221 - AciI CCGC 218 220 . . 243 248 + SfeI* CTRYAG 243 247 . . 250 253 + AciI CCGC 250 252 . . 249 254 - EciI GGCGGA 239 237 . . 261 266 + SduI GDGCHC 265 261 . . 261 266 + HgiAI* GWGCWC 265 261 . . 271 274 + SetI ASST 274 270 . . 277 281 + Tsp45I GTSAC 276 281 . . 277 281 + MaeIII GTNAC 276 281 . . 285 288 + CviRI* TGCA 286 286 . . 306 310 + FokI GGATG 319 323 . . 306 310 + BseGI GGATG 312 310 . . 304 307 - MnlI CCTC 297 296 . . 307 311 - BccI CCATC 301 302 . . 316 319 + DpnI GATC 317 317 . . 316 319 + BstKTI GATC 318 316 . . 316 319 + MboI GATC 315 319 . . 316 320 - BinI* GGATC 310 311 . . 325 329 + BmgT120I GGNCC 326 327 . . 325 329 + AvaII GGWCC 325 328 . . 325 329 + AsuI* GGNCC 325 328 . . 323 326 - AciI CCGC 323 325 . . 327 330 + SetI ASST 330 326 . . 328 331 + MnlI CCTC 338 337 . . 335 339 + TspDTI ATGAA 350 348 . . 348 351 + TaiI ACGT 351 347 . . 348 351 + SetI ASST 351 347 . . 348 351 + MaeII ACGT 348 350 . . 350 354 + Tsp45I GTSAC 349 354 . . 350 354 + MaeIII GTNAC 349 354 . . 354 360 + TspRI CASTGNN 360 351 . . 362 366 + Tsp4CI* ACNGT 364 363 . . 365 369 + Tsp45I GTSAC 364 369 . . 365 369 + MaeIII GTNAC 364 369 . . 391 395 + Hpy188I TCNGA 393 392 . . 394 397 + DpnI GATC 395 395 . . 394 397 + BstKTI GATC 396 394 . . 394 397 + MboI GATC 393 397 . . 387 397 - Hin4I GAYNNNNNVTC 410 405 378 373 417 422 + MmeI TCCRAC 442 440 . . 427 431 + MaeIII GTNAC 426 431 . . 430 434 + Tsp4CI* ACNGT 432 431 . . 447 450 + Csp6I GTAC 447 449 . . 447 450 + RsaI GTAC 448 448 . . 451 454 + MseI TTAA 451 453 . . 467 477 + MwoI GCNNNNNNNGC 473 470 . . 472 476 + Hpy99I CGWCG 476 471 . . 473 478 + AcyI GRCGYC 474 476 . . 476 480 - BceAI ACGGC 461 463 . . #--------------------------------------- # # # AciI: # [CCGC] # 32 73 160 218 # # AcyI: # [GRCGYC] # 9 474 # # AluI: # [AGCT] # 27 52 404 # # AsuI*: # [GGNCC] # 158 325 # # AvaII: # [GGWCC] # 158 325 # # BarI: # [GAAGNNNNNNTAC] # 32 68 383 # # BbvII*: # [GAAGAC] # 30 453 # # BccI: # [CCATC] # 100 182 201 # # BceAI: # [ACGGC] # 22 461 # # BinI*: # [GGATC] # 173 310 # # BmgT120I: # [GGNCC] # 157 326 # # BmtI: # [GCTAGC] # 52 431 # # Bpu10I: # [CCTNAGC] # 63 420 # # BseGI: # [GGATG] # 171 312 # # BsrI: # [ACTGG] # 68 415 # # BstKTI: # [GATC] # 78 87 318 # # BstXI: # [CCANNNNNNTGG] # 159 324 # # Cac8I: # [GCNNGC] # 31 50 402 # # Csp6I: # [GTAC] # 36 61 71 315 # # CviJI: # [RGCY] # 12 14 15 52 86 304 # # CviRI*: # [TGCA] # 197 286 # # DdeI: # [CTNAG] # 63 420 # # DpnI: # [GATC] # 78 88 317 # # EciI: # [GGCGGA] # 239 244 # # FokI: # [GGATG] # 164 319 # # GlaI: # [GCGC] # 47 436 # # HaeII: # [RGCGCY] # 49 434 # # HgiAI*: # [GWGCWC] # 218 265 # # HhaI: # [GCGC] # 48 435 # # Hin4I: # [GAYNNNNNVTC] # 32 73 378 # # Hin6I: # [GCGC] # 46 437 # # HindIII: # [AAGCTT] # 77 406 # # Hpy166II: # [GTNNAC] # 134 349 # # Hpy188I: # [TCNGA] # 90 393 # # Hpy99I: # [CGWCG] # 7 476 # # MaeI: # [CTAG] # 49 434 # # MaeII: # [ACGT] # 135 348 # # MaeIII: # [GTNAC] # 15 57 62 73 276 # # MboI: # [GATC] # 78 90 315 # # MboII: # [GAAGA] # 30 85 368 # # MmeI: # [TCCRAC] # 41 442 # # MnlI: # [CCTC] # 41 145 297 # # MseI: # [TTAA] # 32 119 332 # # MwoI: # [GCNNNNNNNGC] # 10 473 # # NheI: # [GCTAGC] # 48 435 # # NlaIV: # [GGNNCC] # 46 164 273 # # RsaI: # [GTAC] # 35 61 72 315 # # SduI: # [GDGCHC] # 218 265 # # SetI: # [ASST] # 10 17 21 54 56 64 # 129 132 # # SfeI*: # [CTRYAG] # 240 243 # # SspI: # [AATATT] # 117 366 # # TaiI: # [ACGT] # 132 351 # # TatI: # [WGTACW] # 61 70 352 # # TsoI: # [TARCCA] # 81 402 # # Tsp45I: # [GTSAC] # 15 73 119 276 # # Tsp4CI*: # [ACNGT] # 51 68 364 # # TspDTI: # [ATGAA] # 70 127 133 153 # # TspEI: # [AATT] # 120 363 # # TspGWI: # [ACGGA] # 6 195 282 # # TspRI: # [CASTGNN] # 123 360 # # VspI: # [ATTAAT] # 119 364 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 483 # Reported_sequences: 1 # Reported_hitcount: 97 #---------------------------------------