Restriction Fragments for ACT1/YFL039C



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The currently selected gene/sequence is :

ACT1/YFL039C on chromosome VI from coordinates 54696 to 53260.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.25541.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 8 + TfiI GAWTC 4 7 . . 4 8 + HinfI GANTC 4 7 . . 18 21 + MaeI CTAG 18 20 . . 20 25 + HaeII RGCGCY 24 20 . . 20 25 + Eco47III AGCGCT 22 22 . . 21 24 + Hin6I GCGC 21 23 . . 21 24 + GlaI GCGC 22 22 . . 21 24 + HhaI GCGC 23 21 . . 23 28 + Cac8I GCNNGC 25 25 . . 26 29 + CviRI* TGCA 27 27 . . 30 34 + BccI CCATC 38 39 . . 31 35 - FokI GGATG 17 21 . . 31 35 - BseGI GGATG 30 28 . . 39 42 + MseI TTAA 39 41 . . 42 46 + Tsp4CI* ACNGT 44 43 . . 49 53 + MboII GAAGA 61 60 . . 53 56 + TspEI AATT 52 56 . . 56 59 + CviRI* TGCA 57 57 . . 59 63 + Tsp4CI* ACNGT 61 60 . . 61 66 + NlaIV GGNNCC 63 63 . . 61 65 + BmgT120I GGNCC 62 63 . . 61 65 + AvaII GGWCC 61 64 . . 61 65 + AsuI* GGNCC 61 64 . . 66 71 + MfeI CAATTG 66 70 . . 62 66 - BslFI GGGAC 47 51 . . 67 70 + TspEI AATT 66 70 . . 72 77 + SmlI CTYRAG 72 76 . . 72 77 + XhoI CTCGAG 72 76 . . 72 77 + BmeT110I CYCGRG 73 75 . . 72 77 + AvaI CYCGRG 72 76 . . 73 76 + TaqI TCGA 73 75 . . 73 78 + Hpy178III* TCNNGA 74 76 . . 91 94 + SetI ASST 94 90 . . 122 125 + SetI ASST 125 121 . . 123 126 + MnlI CCTC 133 132 . . 138 141 + DpnI GATC 139 139 . . 138 141 + BstKTI GATC 140 138 . . 138 141 + MboI GATC 137 141 . . 147 152 + TsoI TARCCA 163 161 . . 150 164 + XcmI CCANNNNNNNNNTGG 157 156 . . 175 178 + CviJI RGCY 176 176 . . 181 186 + ApoI RAATTY 181 185 . . 182 185 + TspEI AATT 181 185 . . 207 212 + SspI AATATT 209 209 . . 214 219 + Hpy178III* TCNNGA 215 217 . . 236 240 - MboII GAAGA 228 227 . . 252 256 + Tsp4CI* ACNGT 254 253 . . 255 259 + MaeIII GTNAC 254 259 . . 259 263 + DdeI CTNAG 259 262 . . 263 267 + BsmAI GTCTC 268 272 . . 267 270 + FatI CATG 266 270 . . 267 270 + NlaIII CATG 270 266 . . 267 270 + CviAII CATG 267 269 . . 269 274 + TatI WGTACW 269 273 . . 270 273 + Csp6I GTAC 270 272 . . 270 273 + RsaI GTAC 271 271 . . 278 283 + ClaI ATCGAT 279 281 . . 279 282 + TaqI TCGA 279 281 . . 287 291 - TspDTI ATGAA 277 275 . . 320 323 + SetI ASST 323 319 . . 319 322 - MnlI CCTC 312 311 . . 325 329 + BlsI GCNGC 327 326 . . 325 329 + BisI GCNGC 326 327 . . 325 329 + TseI GCWGC 325 328 . . 325 329 - BbvI GCAGC 312 316 . . 332 337 - TsoI TARCCA 322 320 . . 344 348 + Tsp4CI* ACNGT 346 345 . . 363 366 + CviJI RGCY 364 364 . . 364 374 + MwoI GCNNNNNNNGC 370 367 . . 364 369 + Cfr10I RCCGGY 364 368 . . 365 368 + HpaII CCGG 365 367 . . 373 378 + Cfr10I RCCGGY 373 377 . . 374 377 + HpaII CCGG 374 376 . . 376 380 + HphI GGTGA 388 387 . . 377 381 + Tsp45I GTSAC 376 381 . . 377 381 + MaeIII GTNAC 376 381 . . 381 385 + Hpy99I CGWCG 385 380 . . 382 386 + HgaI GACGC 391 396 . . 385 395 + MwoI GCNNNNNNNGC 391 388 . . 388 391 + MnlI CCTC 398 397 . . 386 391 - BseRI GAGGAG 377 375 . . 389 394 - BsiI* CACGAG 389 393 . . 398 402 - MboII GAAGA 390 389 . . 397 402 - BbvII* GAAGAC 390 394 . . 403 407 + BccI CCATC 411 412 . . 411 415 + Hpy99I CGWCG 415 410 . . 416 421 + Hpy166II GTNNAC 418 418 . . 416 421 + AccI GTMKAC 417 419 . . 428 438 + BaeI ACNNNNGTAYC 417 412 450 445 429 434 + StyI CCWWGG 429 433 . . 429 434 + SecI* CCNNGG 429 433 . . 432 435 + SetI ASST 435 431 . . 438 441 + FatI CATG 437 441 . . 438 441 + NlaIII CATG 441 437 . . 438 441 + CviAII CATG 438 440 . . 460 464 + HinfI GANTC 460 463 . . 460 464 - PleI GAGTC 454 455 . . 460 464 - MlyI GAGTC 454 454 . . 467 470 + TaiI ACGT 470 466 . . 467 470 + SetI ASST 470 466 . . 467 470 + MaeII ACGT 467 469 . . 472 476 + HphI GGTGA 484 483 . . 474 485 + BdaI TGANNNNNNTCA 463 461 497 495 476 480 + TspDTI ATGAA 491 489 . . 480 483 + SetI ASST 483 479 . . 480 483 + CviJI RGCY 481 481 . . 480 483 + AluI AGCT 481 481 . . 474 485 - BdaI TGANNNNNNTCA 497 495 463 461 495 498 + SetI ASST 498 494 . . 494 497 - MnlI CCTC 487 486 . . 500 505 + Hpy178III* TCNNGA 501 503 . . 510 513 + TaiI ACGT 513 509 . . 510 513 + SetI ASST 513 509 . . 510 513 + MaeII ACGT 510 512 . . 512 516 + MaeIII GTNAC 511 516 . . 518 523 + MfeI CAATTG 518 522 . . 519 522 + TspEI AATT 518 522 . . 527 531 + Tsp4CI* ACNGT 529 528 . . 535 539 + Tsp45I GTSAC 534 539 . . 535 539 + MaeIII GTNAC 534 539 . . 536 540 - HphI GGTGA 528 527 . . 542 546 + BsrI ACTGG 547 545 . . 542 547 + BfiI ACTGGG 552 551 . . 545 549 + BslFI GGGAC 559 563 . . 560 565 + XhoII RGATCY 560 564 . . 560 565 + BglII AGATCT 560 564 . . 561 571 + Hin4I GAYNNNNNVTC 552 547 584 579 561 564 + DpnI GATC 562 562 . . 561 564 + BstKTI GATC 563 561 . . 561 564 + MboI GATC 560 564 . . 567 571 + SfaNI GCATC 576 580 . . 561 571 - Hin4I GAYNNNNNVTC 584 579 552 547 574 577 + SetI ASST 577 573 . . 575 579 + Hin4II* CCTTC 585 584 . . 587 590 + TspEI AATT 586 590 . . 604 608 + MboII GAAGA 616 615 . . 624 634 + Hin4I GAYNNNNNVTC 615 610 647 642 625 635 + BsaXI ACNNNNNCTCC 615 612 645 642 626 631 + Eco57MI CTGRAG 647 645 . . 626 631 + Eco57I CTGAAG 647 645 . . 630 633 + SetI ASST 633 629 . . 630 633 + CviJI RGCY 631 631 . . 630 633 + AluI AGCT 631 631 . . 637 641 + TspDTI ATGAA 652 650 . . 666 670 + HinfI GANTC 666 669 . . 666 670 - PleI GAGTC 660 661 . . 666 670 - MlyI GAGTC 660 660 . . 672 675 + TspEI AATT 671 675 . . 691 696 + AclI AACGTT 692 694 . . 692 695 + TaiI ACGT 695 691 . . 692 695 + SetI ASST 695 691 . . 692 695 + MaeII ACGT 692 694 . . 699 702 + CviJI RGCY 700 700 . . 701 705 + Hin4II* CCTTC 711 710 . . 707 710 + TaiI ACGT 710 706 . . 707 710 + SetI ASST 710 706 . . 707 710 + MaeII ACGT 707 709 . . 713 717 + BccI CCATC 721 722 . . 714 718 - FokI GGATG 700 704 . . 714 718 - BseGI GGATG 713 711 . . 720 723 + CviJI RGCY 721 721 . . 721 725 - BceAI ACGGC 706 708 . . 734 739 + TatI WGTACW 734 738 . . 735 738 + Csp6I GTAC 735 737 . . 735 738 + RsaI GTAC 736 736 . . 738 743 + Ksp632I* CTCTTC 744 747 . . 742 747 + BetI* WCCGGW 742 746 . . 739 743 - MboII GAAGA 731 730 . . 743 746 + HpaII CCGG 743 745 . . 754 758 + BsrI ACTGG 759 757 . . 769 773 + TfiI GAWTC 769 772 . . 769 773 + HinfI GANTC 769 772 . . 772 777 + BetI* WCCGGW 772 776 . . 773 776 + HpaII CCGG 773 775 . . 775 779 + HphI GGTGA 787 786 . . 778 782 - BccI CCATC 772 773 . . 784 788 + MaeIII GTNAC 783 788 . . 790 795 + BtrI CACGTC 792 792 . . 791 794 + TaiI ACGT 794 790 . . 791 794 + SetI ASST 794 790 . . 791 794 + MaeII ACGT 791 793 . . 792 796 + Hpy99I CGWCG 796 791 . . 801 804 + TspEI AATT 800 804 . . 822 825 + SetI ASST 825 821 . . 823 826 + MnlI CCTC 833 832 . . 839 843 + TfiI GAWTC 839 842 . . 839 843 + HinfI GANTC 839 842 . . 841 846 + ClaI ATCGAT 842 844 . . 842 845 + TaqI TCGA 842 844 . . 848 853 + CfrI YGGCCR 848 852 . . 849 852 + CviJI RGCY 850 850 . . 849 852 + HaeIII GGCC 850 850 . . 850 855 + Cfr10I RCCGGY 850 854 . . 851 854 + HpaII CCGG 851 853 . . 876 885 + BsaBI GATNNNNATC 880 880 . . 877 881 + TspDTI ATGAA 892 890 . . 879 883 + MboII GAAGA 891 890 . . 881 886 + XhoII RGATCY 881 885 . . 881 886 + BglII AGATCT 881 885 . . 882 885 + DpnI GATC 883 883 . . 882 885 + BstKTI GATC 884 882 . . 882 885 + MboI GATC 881 885 . . 884 889 + Hpy178III* TCNNGA 885 887 . . 885 890 + SmlI CTYRAG 885 889 . . 885 890 + Bce83I* CTTGAG 906 904 . . 890 895 + Hpy166II GTNNAC 892 892 . . 894 897 + TaiI ACGT 897 893 . . 894 897 + SetI ASST 897 893 . . 894 897 + MaeII ACGT 894 896 . . 899 903 + MaeIII GTNAC 898 903 . . 897 902 - TsoI TARCCA 887 885 . . 902 912 + BsaXI ACNNNNNCTCC 892 889 922 919 915 921 + TspRI CASTGNN 921 912 . . 915 920 - BtsI GCAGTG 914 912 . . 917 922 - EcoP15I CAGCAG 889 891 . . 930 933 + TspEI AATT 929 933 . . 926 936 - Hin4I GAYNNNNNVTC 949 944 917 912 938 942 + Tsp45I GTSAC 937 942 . . 938 942 + MaeIII GTNAC 937 942 . . 935 939 - TspGWI ACGGA 925 923 . . 959 963 + MaeIII GTNAC 958 963 . . 962 965 + TaiI ACGT 965 961 . . 962 965 + SetI ASST 965 961 . . 962 965 + MaeII ACGT 962 964 . . 963 967 + Hpy99I CGWCG 967 962 . . 968 973 + StyI CCWWGG 968 972 . . 968 973 + SecI* CCNNGG 968 972 . . 976 981 + AsuII TTCGAA 977 979 . . 977 980 + TaqI TCGA 977 979 . . 989 992 + CviRI* TGCA 990 990 . . 990 1001 + AlfI GCANNNNNNTGC 979 977 1013 1011 995 1000 + NspBII* CMGCKG 997 997 . . 995 998 + AciI CCGC 995 997 . . 997 1001 + BlsI GCNGC 999 998 . . 997 1001 + BisI GCNGC 998 999 . . 997 1001 + TseI GCWGC 997 1000 . . 997 1001 - BbvI GCAGC 984 988 . . 990 1001 - AlfI GCANNNNNNTGC 1013 1011 979 977 1006 1010 - MboII GAAGA 998 997 . . 1013 1018 + MfeI CAATTG 1013 1017 . . 1009 1013 - MboII GAAGA 1001 1000 . . 1014 1017 + TspEI AATT 1013 1017 . . 1035 1040 + Hpy178III* TCNNGA 1036 1038 . . 1039 1043 - BccI CCATC 1033 1034 . . 1061 1065 + MaeIII GTNAC 1060 1065 . . 1070 1074 + TfiI GAWTC 1070 1073 . . 1070 1074 + HinfI GANTC 1070 1073 . . 1073 1077 + Hpy188I TCNGA 1075 1074 . . 1076 1081 + SduI GDGCHC 1080 1076 . . 1076 1081 + HgiJII* GRGCYC 1080 1076 . . 1077 1080 + CviJI RGCY 1078 1078 . . 1078 1088 + MwoI GCNNNNNNNGC 1084 1081 . . 1085 1090 + HindIII AAGCTT 1085 1089 . . 1086 1089 + SetI ASST 1089 1085 . . 1086 1089 + CviJI RGCY 1087 1087 . . 1086 1089 + AluI AGCT 1087 1087 . . 1095 1099 + BccI CCATC 1103 1104 . . 1099 1103 + Hin4II* CCTTC 1109 1108 . . 1096 1100 - FokI GGATG 1082 1086 . . 1096 1100 - BseGI GGATG 1095 1093 . . 1117 1121 + TfiI GAWTC 1117 1120 . . 1117 1121 + HinfI GANTC 1117 1120 . . 1123 1128 + Cfr10I RCCGGY 1123 1127 . . 1124 1127 + HpaII CCGG 1124 1126 . . 1151 1155 + BccI CCATC 1159 1160 . . 1154 1159 + Hpy178III* TCNNGA 1155 1157 . . 1154 1159 + BspHI TCATGA 1154 1158 . . 1155 1158 + FatI CATG 1154 1158 . . 1155 1158 + NlaIII CATG 1158 1154 . . 1155 1158 + CviAII CATG 1155 1157 . . 1156 1160 + TspDTI ATGAA 1171 1169 . . 1169 1172 + TaqI TCGA 1169 1171 . . 1175 1179 - TspGWI ACGGA 1165 1163 . . 1184 1187 + TspEI AATT 1183 1187 . . 1190 1194 + Tsp4CI* ACNGT 1192 1191 . . 1193 1197 + MaeIII GTNAC 1192 1197 . . 1197 1206 + MslI CAYNNNNRTG 1201 1201 . . 1207 1212 + BetI* WCCGGW 1207 1211 . . 1208 1211 + HpaII CCGG 1208 1210 . . 1213 1218 + KpnI GGTACC 1217 1213 . . 1213 1218 + NlaIV GGNNCC 1215 1215 . . 1213 1218 + HgiCI* GGYRCC 1213 1217 . . 1213 1218 + Acc65I GGTACC 1213 1217 . . 1214 1217 + Csp6I GTAC 1214 1216 . . 1214 1217 + RsaI GTAC 1215 1215 . . 1221 1224 + FatI CATG 1220 1224 . . 1221 1224 + NlaIII CATG 1224 1220 . . 1221 1224 + CviAII CATG 1221 1223 . . 1227 1232 + SecI* CCNNGG 1227 1231 . . 1228 1232 + BssKI CCNGG 1227 1232 . . 1228 1232 + BseBI CCWGG 1229 1230 . . 1228 1232 + ScrFI CCNGG 1229 1230 . . 1228 1232 + EcoRII CCWGG 1227 1232 . . 1230 1233 + SetI ASST 1233 1229 . . 1244 1249 + BsmI GAATGC 1250 1248 . . 1247 1250 + CviRI* TGCA 1248 1248 . . 1262 1265 + AciI CCGC 1262 1264 . . 1259 1263 - HphI GGTGA 1251 1250 . . 1269 1272 + CviJI RGCY 1270 1270 . . 1269 1274 + NlaIV GGNNCC 1271 1271 . . 1273 1277 + BccI CCATC 1281 1282 . . 1276 1280 - MboII GAAGA 1268 1267 . . 1281 1284 + FatI CATG 1280 1284 . . 1281 1284 + NlaIII CATG 1284 1280 . . 1281 1284 + CviAII CATG 1281 1283 . . 1282 1286 + TspDTI ATGAA 1297 1295 . . 1286 1289 + SetI ASST 1289 1285 . . 1284 1288 - Hin4II* CCTTC 1278 1277 . . 1289 1294 + Hpy178III* TCNNGA 1290 1292 . . 1293 1303 + Hin4I GAYNNNNNVTC 1284 1279 1316 1311 1293 1296 + DpnI GATC 1294 1294 . . 1293 1296 + BstKTI GATC 1295 1293 . . 1293 1296 + MboI GATC 1292 1296 . . 1296 1301 - BsrDI GCAATG 1295 1293 . . 1303 1306 + MnlI CCTC 1313 1312 . . 1305 1310 + Hpy178III* TCNNGA 1306 1308 . . 1301 1306 - BseRI GAGGAG 1292 1290 . . 1304 1309 - Eco57MI CTGRAG 1289 1287 . . 1304 1309 - GsuI CTGGAG 1289 1287 . . 1316 1321 + TatI WGTACW 1316 1320 . . 1316 1321 + ScaI AGTACT 1318 1318 . . 1317 1320 + Csp6I GTAC 1317 1319 . . 1317 1320 + RsaI GTAC 1318 1318 . . 1321 1325 - TspGWI ACGGA 1311 1309 . . 1325 1330 + Hpy178III* TCNNGA 1326 1328 . . 1328 1340 - AloI GAACNNNNNNTCC 1352 1347 1320 1315 1347 1350 + CviJI RGCY 1348 1348 . . 1360 1363 + SetI ASST 1363 1359 . . 1361 1365 + Hin4II* CCTTC 1371 1370 . . 1364 1369 + MmeI TCCRAC 1389 1387 . . 1366 1377 + CspCI CAANNNNNGTGG 1354 1352 1389 1387 1376 1381 + XhoII RGATCY 1376 1380 . . 1376 1380 + BinI* GGATC 1384 1385 . . 1377 1380 + DpnI GATC 1378 1378 . . 1377 1380 + BstKTI GATC 1379 1377 . . 1377 1380 + MboI GATC 1376 1380 . . 1395 1399 + Hpy99I CGWCG 1399 1394 . . 1405 1409 + BmgT120I GGNCC 1406 1407 . . 1405 1409 + AvaII GGWCC 1405 1408 . . 1405 1409 + AsuI* GGNCC 1405 1408 . . 1408 1412 + BccI CCATC 1416 1417 . . 1417 1422 + Hpy166II GTNNAC 1419 1419 . . 1419 1423 - HphI GGTGA 1411 1410 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 224 1213 # # AccI: # [GTMKAC] # 417 1020 # # AciI: # [CCGC] # 175 267 995 # # AclI: # [AACGTT] # 692 745 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 424 979 # # AloI: # [GAACNNNNNNTCC] # 32 85 1320 # # AluI: # [AGCT] # 150 350 456 481 # # ApoI: # [RAATTY] # 181 1256 # # AsuI*: # [GGNCC] # 32 61 1344 # # AsuII: # [TTCGAA] # 460 977 # # AvaI: # [CYCGRG] # 72 1365 # # AvaII: # [GGWCC] # 32 61 1344 # # BaeI: # [ACNNNNGTAYC] # 33 417 987 # # BbvI: # [GCAGC] # 312 453 672 # # BbvII*: # [GAAGAC] # 390 1047 # # BccI: # [CCATC] # 21 38 51 56 70 122 # 135 261 310 373 # # Bce83I*: # [CTTGAG] # 531 906 # # BceAI: # [ACGGC] # 706 731 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 463 940 # # BetI*: # [WCCGGW] # 30 230 435 742 # # BfiI: # [ACTGGG] # 552 885 # # BglII: # [AGATCT] # 321 556 560 # # BinI*: # [GGATC] # 53 1384 # # BisI: # [GCNGC] # 326 439 672 # # BlsI: # [GCNGC] # 327 438 672 # # BmeT110I: # [CYCGRG] # 73 1364 # # BmgT120I: # [GGNCC] # 31 62 1344 # # BsaBI: # [GATNNNNATC] # 557 880 # # BsaXI: # [ACNNNNNCTCC] # 30 30 247 515 615 # # BseBI: # [CCWGG] # 208 1229 # # BseGI: # [GGATG] # 30 342 382 683 # # BseRI: # [GAGGAG] # 145 377 915 # # BsiI*: # [CACGAG] # 389 1048 # # BslFI: # [GGGAC] # 47 512 878 # # BsmAI: # [GTCTC] # 268 1169 # # BsmI: # [GAATGC] # 187 1250 # # BspHI: # [TCATGA] # 283 1154 # # BsrDI: # [GCAATG] # 142 1295 # # BsrI: # [ACTGG] # 212 547 678 # # BssKI: # [CCNGG] # 210 1227 # # BstKTI: # [GATC] # 58 84 140 321 411 423 # # BtrI: # [CACGTC] # 645 792 # # BtsI: # [GCAGTG] # 523 914 # # Cac8I: # [GCNNGC] # 25 1412 # # Cfr10I: # [RCCGGY] # 9 273 314 364 477 # # CfrI: # [YGGCCR] # 589 848 # # ClaI: # [ATCGAT] # 279 563 595 # # Csp6I: # [GTAC] # 103 120 270 465 479 # # CspCI: # [CAANNNNNGTGG] # 35 48 1354 # # CviAII: # [CATG] # 60 66 156 171 267 717 # # CviJI: # [RGCY] # 9 21 69 78 89 117 # 129 150 176 183 188 228 # # CviRI*: # [TGCA] # 27 30 189 258 933 # # DdeI: # [CTNAG] # 259 1178 # # DpnI: # [GATC] # 59 84 139 321 411 423 # # Eco47III: # [AGCGCT] # 22 1415 # # Eco57I: # [CTGAAG] # 647 790 # # Eco57MI: # [CTGRAG] # 148 642 647 # # EcoP15I: # [CAGCAG] # 548 889 # # EcoRII: # [CCWGG] # 210 1227 # # FatI: # [CATG] # 60 66 157 171 266 717 # # FokI: # [GGATG] # 17 355 382 683 # # GlaI: # [GCGC] # 22 1415 # # GsuI: # [CTGGAG] # 148 1289 # # HaeII: # [RGCGCY] # 24 1413 # # HaeIII: # [GGCC] # 587 850 # # HgaI: # [GACGC] # 391 1046 # # HgiCI*: # [GGYRCC] # 224 1213 # # HgiJII*: # [GRGCYC] # 357 1080 # # HhaI: # [GCGC] # 23 1414 # # Hin4I: # [GAYNNNNNVTC] # 0 0 31 32 32 32 # 32 121 270 335 552 # # Hin4II*: # [CCTTC] # 66 93 126 169 398 585 # # Hin6I: # [GCGC] # 21 1416 # # HindIII: # [AAGCTT] # 352 1085 # # HinfI: # [GANTC] # 4 47 70 103 206 231 # 320 456 # # HpaII: # [CCGG] # 9 30 78 84 229 273 # 365 369 # # HphI: # [GGTGA] # 26 44 96 160 259 388 # 464 # # Hpy166II: # [GTNNAC] # 18 418 474 527 # # Hpy178III*: # [TCNNGA] # 16 20 74 111 119 135 # 141 151 286 384 # # Hpy188I: # [TCNGA] # 362 1075 # # Hpy99I: # [CGWCG] # 30 38 171 381 385 432 # # KpnI: # [GGTACC] # 220 1217 # # Ksp632I*: # [CTCTTC] # 693 744 # # MaeI: # [CTAG] # 18 1419 # # MaeII: # [ACGT] # 15 43 68 84 103 182 # 467 475 # # MaeIII: # [GTNAC] # 21 23 39 102 115 122 # 132 135 245 249 254 # # MboI: # [GATC] # 61 84 137 321 411 423 # # MboII: # [GAAGA] # 3 61 107 115 160 162 # 167 169 226 267 # # MfeI: # [CAATTG] # 66 424 452 495 # # MlyI: # [GAGTC] # 206 454 777 # # MmeI: # [TCCRAC] # 48 1389 # # MnlI: # [CCTC] # 86 89 124 133 179 346 # 480 # # MseI: # [TTAA] # 39 1398 # # MslI: # [CAYNNNNRTG] # 236 1201 # # MwoI: # [GCNNNNNNNGC] # 21 353 370 693 # # NlaIII: # [CATG] # 60 66 153 171 270 717 # # NlaIV: # [GGNNCC] # 56 63 166 1152 # # NspBII*: # [CMGCKG] # 440 997 # # PleI: # [GAGTC] # 206 454 777 # # RsaI: # [GTAC] # 103 119 271 465 479 # # ScaI: # [AGTACT] # 119 1318 # # ScrFI: # [CCNGG] # 208 1229 # # SduI: # [GDGCHC] # 357 1080 # # SecI*: # [CCNNGG] # 210 259 429 539 # # SetI: # [ASST] # 13 15 15 15 31 31 # 35 56 56 62 64 68 # 72 74 74 84 94 112 # 124 144 198 # # SfaNI: # [GCATC] # 576 861 # # SmlI: # [CTYRAG] # 72 552 813 # # SspI: # [AATATT] # 209 1228 # # StyI: # [CCWWGG] # 429 469 539 # # TaiI: # [ACGT] # 15 43 68 84 103 182 # 470 472 # # TaqI: # [TCGA] # 73 135 192 206 268 563 # # TatI: # [WGTACW] # 121 269 465 582 # # TfiI: # [GAWTC] # 4 47 70 231 320 765 # # TseI: # [GCWGC] # 325 440 672 # # TsoI: # [TARCCA] # 159 163 550 565 # # Tsp45I: # [GTSAC] # 158 376 403 500 # # Tsp4CI*: # [ACNGT] # 17 44 92 183 193 245 # 663 # # TspDTI: # [ATGAA] # 126 140 161 214 240 277 # 279 # # TspEI: # [AATT] # 14 52 68 84 85 115 # 129 129 170 254 337 # # TspGWI: # [ACGGA] # 126 146 240 925 # # TspRI: # [CASTGNN] # 516 921 # # XcmI: # [CCANNNNNNNNNTGG] # 157 1280 # # XhoI: # [CTCGAG] # 72 1365 # # XhoII: # [RGATCY] # 61 321 495 560 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1437 # Reported_sequences: 1 # Reported_hitcount: 343 #---------------------------------------