Restriction Fragments for HAC1/YFL031W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

HAC1/YFL031W on chromosome VI from coordinates 75179 to 76147.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.25391.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 25 30 + SpeI ACTAGT 25 29 . . 26 29 + MaeI CTAG 26 28 . . 31 34 + TspEI AATT 30 34 . . 40 43 + TaqI TCGA 40 42 . . 48 51 + CviJI RGCY 49 49 . . 47 52 - TsoI TARCCA 37 35 . . 69 74 + SalI GTCGAC 69 73 . . 69 74 + Hpy166II GTNNAC 71 71 . . 69 74 + HindII GTYRAC 71 71 . . 69 74 + AccI GTMKAC 70 72 . . 70 73 + TaqI TCGA 70 72 . . 72 76 + HinfI GANTC 72 75 . . 72 82 + Hin4I GAYNNNNNVTC 63 58 95 90 73 83 + BsaXI ACNNNNNCTCC 63 60 93 90 72 76 - PleI GAGTC 66 67 . . 72 76 - MlyI GAGTC 66 66 . . 79 82 + MnlI CCTC 89 88 . . 82 87 + StyI CCWWGG 82 86 . . 82 87 + SecI* CCNNGG 82 86 . . 93 96 + CviJI RGCY 94 94 . . 106 109 - MnlI CCTC 99 98 . . 125 129 + BinI* GGATC 133 134 . . 122 126 - Hin4II* CCTTC 116 115 . . 126 129 + DpnI GATC 127 127 . . 126 129 + BstKTI GATC 128 126 . . 126 129 + MboI GATC 125 129 . . 128 131 + TaqI TCGA 128 130 . . 149 153 + MboII GAAGA 161 160 . . 153 156 + SetI ASST 156 152 . . 153 156 + CviJI RGCY 154 154 . . 153 156 + AluI AGCT 154 154 . . 149 154 - Ksp632I* CTCTTC 144 147 . . 154 158 + BlsI GCNGC 156 155 . . 154 158 + BisI GCNGC 155 156 . . 154 158 + TseI GCWGC 154 157 . . 149 155 - SapI GCTCTTC 144 147 . . 154 158 - BbvI GCAGC 141 145 . . 159 163 - HphI GGTGA 151 150 . . 190 195 + PstI CTGCAG 194 190 . . 190 195 + SfeI* CTRYAG 190 194 . . 191 194 + CviRI* TGCA 192 192 . . 198 203 + Hpy178III* TCNNGA 199 201 . . 199 204 + SmlI CTYRAG 199 203 . . 199 204 + XhoI CTCGAG 199 203 . . 199 204 + BmeT110I CYCGRG 200 202 . . 199 204 + AvaI CYCGRG 199 203 . . 200 203 + TaqI TCGA 200 202 . . 200 205 + Hpy178III* TCNNGA 201 203 . . 206 215 + XmnI GAANNNNTTC 210 210 . . 204 215 - PpiI GAACNNNNNCTC 227 222 195 190 225 230 + ApoI RAATTY 225 229 . . 226 229 + TspEI AATT 225 229 . . 233 244 + BdaI TGANNNNNNTCA 222 220 256 254 241 246 + Hpy166II GTNNAC 243 243 . . 241 246 + HindII GTYRAC 243 243 . . 239 243 - HgaI GACGC 228 233 . . 233 244 - BdaI TGANNNNNNTCA 256 254 222 220 245 248 + SetI ASST 248 244 . . 255 259 + BsrI ACTGG 260 258 . . 258 261 + CviJI RGCY 259 259 . . 268 272 + MboII GAAGA 280 279 . . 268 273 + BbvII* GAAGAC 275 279 . . 271 275 + HgaI GACGC 280 285 . . 272 277 + MluI ACGCGT 272 276 . . 272 277 + AflIII ACRYGT 272 276 . . 273 276 + FnuDII* CGCG 274 274 . . 276 281 + Hpy166II GTNNAC 278 278 . . 276 281 + HindII GTYRAC 278 278 . . 283 286 + CviRI* TGCA 284 284 . . 284 288 + BlsI GCNGC 286 285 . . 284 288 + BisI GCNGC 285 286 . . 284 288 + BbvI GCAGC 296 300 . . 284 288 + TseI GCWGC 284 287 . . 286 289 + CviJI RGCY 287 287 . . 291 295 + Hpy99I CGWCG 295 290 . . 292 296 + HgaI GACGC 301 306 . . 295 305 + MwoI GCNNNNNNNGC 301 298 . . 309 314 + Hpy178III* TCNNGA 310 312 . . 317 322 + TatI WGTACW 317 321 . . 318 321 + Csp6I GTAC 318 320 . . 318 321 + RsaI GTAC 319 319 . . 329 334 + Hpy178III* TCNNGA 330 332 . . 333 338 + SduI GDGCHC 337 333 . . 333 338 + HgiAI* GWGCWC 337 333 . . 335 345 + MwoI GCNNNNNNNGC 341 338 . . 336 341 + BsiI* CACGAG 336 340 . . 339 342 - MnlI CCTC 332 331 . . 343 348 + HaeII RGCGCY 347 343 . . 344 347 + Hin6I GCGC 344 346 . . 344 347 + GlaI GCGC 345 345 . . 344 347 + HhaI GCGC 346 344 . . 350 356 + TspRI CASTGNN 356 347 . . 351 355 + BsrI ACTGG 356 354 . . 359 363 + BssKI CCNGG 358 363 . . 359 363 + BseBI CCWGG 360 361 . . 359 364 + SecI* CCNNGG 359 363 . . 359 363 + ScrFI CCNGG 360 361 . . 359 363 + EcoRII CCWGG 358 363 . . 362 366 + BmgT120I GGNCC 363 364 . . 362 366 + AsuI* GGNCC 362 365 . . 363 366 + CviJI RGCY 364 364 . . 363 366 + HaeIII GGCC 364 364 . . 365 369 - BsrI ACTGG 365 363 . . 377 381 + Hpy99I CGWCG 381 376 . . 382 386 + Hpy188I TCNGA 384 383 . . 388 391 + TaiI ACGT 391 387 . . 388 391 + SetI ASST 391 387 . . 388 391 + MaeII ACGT 388 390 . . 390 395 + Hpy166II GTNNAC 392 392 . . 396 399 + SetI ASST 399 395 . . 397 401 + Hin4II* CCTTC 407 406 . . 402 405 + SetI ASST 405 401 . . 403 406 + MnlI CCTC 413 412 . . 400 404 - HphI GGTGA 392 391 . . 405 409 + Hpy188I TCNGA 407 406 . . 410 414 + Tsp4CI* ACNGT 412 411 . . 412 417 + TatI WGTACW 412 416 . . 412 417 + Bsp1407I TGTACA 412 416 . . 413 416 + Csp6I GTAC 413 415 . . 413 416 + RsaI GTAC 414 414 . . 420 425 + NlaIV GGNNCC 422 422 . . 422 425 + CviJI RGCY 423 423 . . 423 428 + Cac8I GCNNGC 425 425 . . 429 437 + Tth111I GACNNNGTC 432 433 . . 450 453 + Hin6I GCGC 450 452 . . 450 453 + GlaI GCGC 451 451 . . 450 453 + HhaI GCGC 452 450 . . 451 454 + FnuDII* CGCG 452 452 . . 454 458 + TfiI GAWTC 454 457 . . 454 458 + HinfI GANTC 454 457 . . 454 464 + Hin4I GAYNNNNNVTC 445 440 477 472 458 461 + AciI CCGC 458 460 . . 459 462 + FnuDII* CGCG 460 460 . . 461 465 + Hpy99I CGWCG 465 460 . . 463 467 + Hpy188I TCNGA 465 464 . . 454 464 - Hin4I GAYNNNNNVTC 477 472 445 440 460 464 - HgaI GACGC 449 454 . . 465 469 + BmgT120I GGNCC 466 467 . . 465 469 + AvaII GGWCC 465 468 . . 465 469 + AsuI* GGNCC 465 468 . . 462 467 - MmeI TCCRAC 443 441 . . 472 476 - BsmAI GTCTC 466 470 . . 479 482 + FatI CATG 478 482 . . 479 482 + NlaIII CATG 482 478 . . 479 482 + CviAII CATG 479 481 . . 477 481 - TspDTI ATGAA 467 465 . . 485 488 + SetI ASST 488 484 . . 485 488 + CviJI RGCY 486 486 . . 485 488 + AluI AGCT 486 486 . . 486 490 + BlsI GCNGC 488 487 . . 486 490 + BisI GCNGC 487 488 . . 486 490 + TseI GCWGC 486 489 . . 487 492 + PstI CTGCAG 491 487 . . 487 492 + SfeI* CTRYAG 487 491 . . 488 491 + CviRI* TGCA 489 489 . . 486 490 - BbvI GCAGC 473 477 . . 497 500 + MseI TTAA 497 499 . . 502 506 + TspGWI ACGGA 517 515 . . 511 514 + Csp6I GTAC 511 513 . . 511 514 + RsaI GTAC 512 512 . . 517 521 + PleI GAGTC 525 526 . . 517 521 + MlyI GAGTC 526 526 . . 517 521 + HinfI GANTC 517 520 . . 519 524 + SalI GTCGAC 519 523 . . 519 524 + Hpy166II GTNNAC 521 521 . . 519 524 + HindII GTYRAC 521 521 . . 519 524 + AccI GTMKAC 520 522 . . 520 523 + TaqI TCGA 520 522 . . 521 525 + Hpy99I CGWCG 525 520 . . 524 528 + Hpy99I CGWCG 528 523 . . 525 529 + HgaI GACGC 534 539 . . 531 534 + SetI ASST 534 530 . . 531 536 + BspMI ACCTGC 540 544 . . 538 543 + Hpy166II GTNNAC 540 540 . . 538 543 + AccI GTMKAC 539 541 . . 535 539 - BceAI ACGGC 520 522 . . 550 553 + TspEI AATT 549 553 . . 559 563 - SfaNI GCATC 549 553 . . 562 565 - AciI CCGC 562 564 . . 567 570 + CviJI RGCY 568 568 . . 567 570 + HaeIII GGCC 568 568 . . 569 572 + MnlI CCTC 579 578 . . 573 577 - BceAI ACGGC 558 560 . . 594 598 + Hpy99I CGWCG 598 593 . . 604 607 - AciI CCGC 604 606 . . 604 608 - FauI CCCGC 597 599 . . 611 615 + Tsp4CI* ACNGT 613 612 . . 614 619 + Hpy166II GTNNAC 616 616 . . 614 619 + AccI GTMKAC 615 617 . . 628 631 + TspEI AATT 627 631 . . 632 637 + MfeI CAATTG 632 636 . . 633 636 + TspEI AATT 632 636 . . 637 640 + DpnI GATC 638 638 . . 637 640 + BstKTI GATC 639 637 . . 637 640 + MboI GATC 636 640 . . 639 644 + Hpy178III* TCNNGA 640 642 . . 645 650 + MfeI CAATTG 645 649 . . 646 649 + TspEI AATT 645 649 . . 660 663 + CviJI RGCY 661 661 . . 661 665 - BceAI ACGGC 646 648 . . 677 681 + BssKI CCNGG 676 681 . . 677 681 + BseBI CCWGG 678 679 . . 677 681 + ScrFI CCNGG 678 679 . . 677 681 + EcoRII CCWGG 676 681 . . 685 690 + SfeI* CTRYAG 685 689 . . 687 691 + Tsp4CI* ACNGT 689 688 . . 688 694 + TspRI CASTGNN 694 685 . . 690 695 + Hpy166II GTNNAC 692 692 . . 703 706 + MaeI CTAG 703 705 . . 705 708 + CviJI RGCY 706 706 . . 708 713 + GsaI CCCAGC 712 708 . . 708 713 + BseYI CCCAGC 708 712 . . 711 714 + SetI ASST 714 710 . . 711 714 + CviJI RGCY 712 712 . . 711 714 + AluI AGCT 712 712 . . 761 765 + Hpy188I TCNGA 763 762 . . 764 768 - BccI CCATC 758 759 . . 771 776 + BseRI GAGGAG 786 784 . . 773 778 + NlaIV GGNNCC 775 775 . . 771 774 - MnlI CCTC 764 763 . . 775 778 + CviJI RGCY 776 776 . . 776 781 + Cfr10I RCCGGY 776 780 . . 777 780 + HpaII CCGG 777 779 . . 781 784 + MseI TTAA 781 783 . . 786 789 + Csp6I GTAC 786 788 . . 786 789 + RsaI GTAC 787 787 . . 788 791 + SetI ASST 791 787 . . 789 793 + Hin4II* CCTTC 799 798 . . 801 806 + BsmI GAATGC 807 805 . . 804 807 + CviRI* TGCA 805 805 . . 818 823 + HindIII AAGCTT 818 822 . . 819 822 + SetI ASST 822 818 . . 819 822 + CviJI RGCY 820 820 . . 819 822 + AluI AGCT 820 820 . . 841 844 + MaeI CTAG 841 843 . . 849 854 + Hpy178III* TCNNGA 850 852 . . 863 866 + CviJI RGCY 864 864 . . 882 886 + DdeI CTNAG 882 885 . . 893 897 + BceAI ACGGC 909 911 . . 895 898 + CviJI RGCY 896 896 . . 896 901 + Cac8I GCNNGC 898 898 . . 903 908 + TatI WGTACW 903 907 . . 904 907 + Csp6I GTAC 904 906 . . 904 907 + RsaI GTAC 905 905 . . 906 910 + Tsp4CI* ACNGT 908 907 . . 910 914 + Hpy188I TCNGA 912 911 . . 916 919 + Hin6I GCGC 916 918 . . 916 919 + GlaI GCGC 917 917 . . 916 919 + HhaI GCGC 918 916 . . 924 927 + SetI ASST 927 923 . . 930 935 + EcoRI GAATTC 930 934 . . 930 935 + ApoI RAATTY 930 934 . . 931 934 + TspEI AATT 930 934 . . 933 937 - TspDTI ATGAA 923 921 . . 941 944 + TspEI AATT 940 944 . . 945 954 + XmnI GAANNNNTTC 949 949 . . 953 957 - MboII GAAGA 945 944 . . 955 959 - TspDTI ATGAA 945 943 . . 964 969 + Hpy178III* TCNNGA 965 967 . . 964 969 + BspHI TCATGA 964 968 . . 965 968 + FatI CATG 964 968 . . 965 968 + NlaIII CATG 968 964 . . 965 968 + CviAII CATG 965 967 . . 963 967 - TspDTI ATGAA 953 951 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 19 70 76 354 450 # # AciI: # [CCGC] # 42 104 365 458 # # AflIII: # [ACRYGT] # 272 697 # # AluI: # [AGCT] # 108 149 154 226 332 # # ApoI: # [RAATTY] # 39 225 705 # # AsuI*: # [GGNCC] # 103 362 504 # # AvaI: # [CYCGRG] # 199 770 # # AvaII: # [GGWCC] # 465 504 # # BbvI: # [GCAGC] # 141 155 177 496 # # BbvII*: # [GAAGAC] # 275 694 # # BccI: # [CCATC] # 211 758 # # BceAI: # [ACGGC] # 38 60 88 263 520 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 222 713 # # BinI*: # [GGATC] # 133 836 # # BisI: # [GCNGC] # 130 155 202 482 # # BlsI: # [GCNGC] # 130 156 202 481 # # BmeT110I: # [CYCGRG] # 200 769 # # BmgT120I: # [GGNCC] # 103 363 503 # # BsaXI: # [ACNNNNNCTCC] # 30 63 876 # # BseBI: # [CCWGG] # 291 318 360 # # BseRI: # [GAGGAG] # 183 786 # # BseYI: # [CCCAGC] # 261 708 # # BsiI*: # [CACGAG] # 336 633 # # BsmAI: # [GTCTC] # 466 503 # # BsmI: # [GAATGC] # 162 807 # # Bsp1407I: # [TGTACA] # 412 557 # # BspHI: # [TCATGA] # 5 964 # # BspMI: # [ACCTGC] # 429 540 # # BsrI: # [ACTGG] # 9 96 260 604 # # BssKI: # [CCNGG] # 293 318 358 # # BstKTI: # [GATC] # 128 330 511 # # Cac8I: # [GCNNGC] # 71 425 473 # # Cfr10I: # [RCCGGY] # 193 776 # # Csp6I: # [GTAC] # 65 95 98 118 275 318 # # CviAII: # [CATG] # 4 479 486 # # CviJI: # [RGCY] # 6 28 32 44 44 45 # 45 49 59 60 63 64 # 73 77 82 93 105 # # CviRI*: # [TGCA] # 92 164 192 205 316 # # DdeI: # [CTNAG] # 87 882 # # DpnI: # [GATC] # 127 331 511 # # EcoRI: # [GAATTC] # 39 930 # # EcoRII: # [CCWGG] # 293 318 358 # # FatI: # [CATG] # 5 478 486 # # FauI: # [CCCGC] # 372 597 # # FnuDII*: # [CGCG] # 8 178 274 509 # # GlaI: # [GCGC] # 52 106 345 466 # # GsaI: # [CCCAGC] # 257 712 # # HaeII: # [RGCGCY] # 347 622 # # HaeIII: # [GGCC] # 204 364 401 # # HgaI: # [GACGC] # 21 52 85 148 228 435 # # HgiAI*: # [GWGCWC] # 337 632 # # HhaI: # [GCGC] # 51 106 346 466 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 32 63 350 # 492 # # Hin4II*: # [CCTTC] # 116 170 291 392 # # Hin6I: # [GCGC] # 53 106 344 466 # # HindII: # [GTYRAC] # 35 71 172 243 448 # # HindIII: # [AAGCTT] # 151 818 # # HinfI: # [GANTC] # 63 72 382 452 # # HpaII: # [CCGG] # 192 777 # # HphI: # [GGTGA] # 151 241 577 # # Hpy166II: # [GTNNAC] # 19 35 71 76 76 114 # 129 172 277 # # Hpy178III*: # [TCNNGA] # 2 4 20 109 115 199 # 210 310 # # Hpy188I: # [TCNGA] # 23 57 58 149 298 384 # # Hpy99I: # [CGWCG] # 3 60 70 84 86 295 # 371 # # Ksp632I*: # [CTCTTC] # 144 825 # # MaeI: # [CTAG] # 26 128 138 677 # # MaeII: # [ACGT] # 388 581 # # MboI: # [GATC] # 125 333 511 # # MboII: # [GAAGA] # 24 119 161 665 # # MfeI: # [CAATTG] # 13 324 632 # # MluI: # [ACGCGT] # 272 697 # # MlyI: # [GAGTC] # 66 443 460 # # MmeI: # [TCCRAC] # 443 526 # # MnlI: # [CCTC] # 10 81 89 166 185 205 # 233 # # MseI: # [TTAA] # 188 284 497 # # MwoI: # [GCNNNNNNNGC] # 40 301 628 # # NlaIII: # [CATG] # 1 482 486 # # NlaIV: # [GGNNCC] # 194 353 422 # # PleI: # [GAGTC] # 66 444 459 # # PpiI: # [GAACNNNNNCTC] # 32 195 742 # # PstI: # [CTGCAG] # 194 297 478 # # RsaI: # [GTAC] # 64 95 98 118 275 319 # # SalI: # [GTCGAC] # 69 450 450 # # SapI: # [GCTCTTC] # 144 825 # # ScrFI: # [CCNGG] # 291 318 360 # # SduI: # [GDGCHC] # 337 632 # # SecI*: # [CCNNGG] # 82 277 610 # # SetI: # [ASST] # 6 8 31 42 46 77 # 83 92 105 143 156 180 # # SfaNI: # [GCATC] # 420 549 # # SfeI*: # [CTRYAG] # 190 198 284 297 # # SmlI: # [CTYRAG] # 199 770 # # SpeI: # [ACTAGT] # 25 944 # # StyI: # [CCWWGG] # 82 887 # # TaiI: # [ACGT] # 391 578 # # TaqI: # [TCGA] # 30 40 58 72 320 449 # # TatI: # [WGTACW] # 66 95 317 491 # # TfiI: # [GAWTC] # 454 515 # # TseI: # [GCWGC] # 130 154 202 483 # # TsoI: # [TARCCA] # 37 932 # # Tsp4CI*: # [ACNGT] # 61 76 201 219 412 # # TspDTI: # [ATGAA] # 8 16 22 456 467 # # TspEI: # [AATT] # 5 10 13 29 30 78 # 195 285 324 # # TspGWI: # [ACGGA] # 452 517 # # TspRI: # [CASTGNN] # 275 338 356 # # Tth111I: # [GACNNNGTC] # 432 537 # # XhoI: # [CTCGAG] # 199 770 # # XmnI: # [GAANNNNTTC] # 20 210 739 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 969 # Reported_sequences: 1 # Reported_hitcount: 264 #---------------------------------------