Restriction Fragments for YERCsigma3



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YERCsigma3 on chromosome V from coordinates 434971 to 434631.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.11350.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 27 + BdaI TGANNNNNNTCA 5 3 39 37 22 25 + TaiI ACGT 25 21 . . 22 25 + SetI ASST 25 21 . . 22 25 + MaeII ACGT 22 24 . . 16 27 - BdaI TGANNNNNNTCA 39 37 5 3 19 29 - BaeI ACNNNNGTAYC 44 39 11 6 32 42 + Hin4I GAYNNNNNVTC 23 18 55 50 32 42 + Eam1105I GACNNNNNGTC 37 36 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 32 42 - Hin4I GAYNNNNNVTC 55 50 23 18 42 46 - FokI GGATG 28 32 . . 42 46 - BseGI GGATG 41 39 . . 49 54 + AclI AACGTT 50 52 . . 50 53 + TaiI ACGT 53 49 . . 50 53 + SetI ASST 53 49 . . 50 53 + MaeII ACGT 50 52 . . 53 57 - TspDTI ATGAA 43 41 . . 64 69 + NdeI CATATG 65 67 . . 67 71 + TspDTI ATGAA 82 80 . . 75 78 + SetI ASST 78 74 . . 78 83 + PsiI TTATAA 80 80 . . 96 99 + FatI CATG 95 99 . . 96 99 + NlaIII CATG 99 95 . . 96 99 + CviAII CATG 96 98 . . 108 112 + BssKI CCNGG 107 112 . . 108 112 + ScrFI CCNGG 109 110 . . 108 112 + CauII* CCSGG 109 110 . . 109 112 + HpaII CCGG 109 111 . . 111 114 + CviJI RGCY 112 112 . . 111 114 + HaeIII GGCC 112 112 . . 113 117 + Hin4II* CCTTC 123 122 . . 119 122 + MaeI CTAG 119 121 . . 121 124 + SetI ASST 124 120 . . 121 124 + CviJI RGCY 122 122 . . 121 124 + AluI AGCT 122 122 . . 154 157 + TspEI AATT 153 157 . . 163 168 + XhoII RGATCY 163 167 . . 164 167 + DpnI GATC 165 165 . . 164 167 + BstKTI GATC 166 164 . . 164 167 + MboI GATC 163 167 . . 166 170 + Hpy188I TCNGA 168 167 . . 164 168 - BinI* GGATC 158 159 . . 171 175 + TfiI GAWTC 171 174 . . 171 175 + HinfI GANTC 171 174 . . 175 178 + AciI CCGC 175 177 . . 176 179 + FnuDII* CGCG 177 177 . . 177 180 + Hin6I GCGC 177 179 . . 177 180 + GlaI GCGC 178 178 . . 177 180 + HhaI GCGC 179 177 . . 189 193 + DdeI CTNAG 189 192 . . 197 201 + TfiI GAWTC 197 200 . . 197 201 + HinfI GANTC 197 200 . . 199 203 - TspDTI ATGAA 189 187 . . 227 231 + MaeIII GTNAC 226 231 . . 234 238 - TspGWI ACGGA 224 222 . . 240 243 + TspEI AATT 239 243 . . 241 246 + VspI ATTAAT 242 244 . . 242 245 + MseI TTAA 242 244 . . 246 250 + Hpy188I TCNGA 248 247 . . 254 258 + Tsp4CI* ACNGT 256 255 . . 266 270 + BsrI ACTGG 271 269 . . 270 274 + MaeIII GTNAC 269 274 . . 268 273 - TsoI TARCCA 258 256 . . 279 283 + DdeI CTNAG 279 282 . . 284 288 + Tsp4CI* ACNGT 286 285 . . 303 308 + Hpy178III* TCNNGA 304 306 . . 312 317 + BetI* WCCGGW 312 316 . . 313 316 + HpaII CCGG 313 315 . . 321 328 + PspXI VCTCGAGB 322 326 . . 322 327 + SmlI CTYRAG 322 326 . . 322 327 + XhoI CTCGAG 322 326 . . 322 327 + BmeT110I CYCGRG 323 325 . . 322 327 + AvaI CYCGRG 322 326 . . 323 326 + TaqI TCGA 323 325 . . 325 330 + SduI GDGCHC 329 325 . . 325 330 + HgiJII* GRGCYC 329 325 . . 326 329 + CviJI RGCY 327 327 . . #--------------------------------------- # # # AciI: # [CCGC] # 166 175 # # AclI: # [AACGTT] # 50 291 # # AluI: # [AGCT] # 122 219 # # AvaI: # [CYCGRG] # 19 322 # # BaeI: # [ACNNNNGTAYC] # 11 33 297 # # BdaI: # [TGANNNNNNTCA] # 0 0 5 34 302 # # BetI*: # [WCCGGW] # 29 312 # # BinI*: # [GGATC] # 158 183 # # BmeT110I: # [CYCGRG] # 18 323 # # BseGI: # [GGATG] # 41 300 # # BsrI: # [ACTGG] # 70 271 # # BssKI: # [CCNGG] # 107 234 # # BstKTI: # [GATC] # 166 175 # # CauII*: # [CCSGG] # 109 232 # # CviAII: # [CATG] # 96 245 # # CviJI: # [RGCY] # 10 14 112 205 # # DdeI: # [CTNAG] # 62 90 189 # # DpnI: # [GATC] # 165 176 # # Eam1105I: # [GACNNNNNGTC] # 37 304 # # FatI: # [CATG] # 95 246 # # FnuDII*: # [CGCG] # 164 177 # # FokI: # [GGATG] # 28 313 # # GlaI: # [GCGC] # 163 178 # # HaeIII: # [GGCC] # 112 229 # # HgiJII*: # [GRGCYC] # 12 329 # # HhaI: # [GCGC] # 162 179 # # Hin4I: # [GAYNNNNNVTC] # 0 0 23 32 286 # # Hin4II*: # [CCTTC] # 123 218 # # Hin6I: # [GCGC] # 164 177 # # HinfI: # [GANTC] # 26 144 171 # # HpaII: # [CCGG] # 28 109 204 # # Hpy178III*: # [TCNNGA] # 37 304 # # Hpy188I: # [TCNGA] # 80 93 168 # # MaeI: # [CTAG] # 119 222 # # MaeII: # [ACGT] # 12 16 22 291 # # MaeIII: # [GTNAC] # 43 72 226 # # MboI: # [GATC] # 163 178 # # MseI: # [TTAA] # 99 242 # # NdeI: # [CATATG] # 65 276 # # NlaIII: # [CATG] # 99 242 # # PsiI: # [TTATAA] # 80 261 # # PspXI: # [VCTCGAGB] # 19 322 # # ScrFI: # [CCNGG] # 109 232 # # SduI: # [GDGCHC] # 12 329 # # SetI: # [ASST] # 12 16 25 25 46 217 # # SmlI: # [CTYRAG] # 19 322 # # TaiI: # [ACGT] # 12 16 25 288 # # TaqI: # [TCGA] # 18 323 # # TfiI: # [GAWTC] # 26 144 171 # # TsoI: # [TARCCA] # 83 258 # # Tsp4CI*: # [ACNGT] # 30 55 256 # # TspDTI: # [ATGAA] # 39 43 107 152 # # TspEI: # [AATT] # 86 102 153 # # TspGWI: # [ACGGA] # 117 224 # # VspI: # [ATTAAT] # 99 242 # # XhoI: # [CTCGAG] # 19 322 # # XhoII: # [RGATCY] # 163 178 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 341 # Reported_sequences: 1 # Reported_hitcount: 79 #---------------------------------------