Restriction Fragments for SPT15/YER148W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

SPT15/YER148W on chromosome V from coordinates 465303 to 466025.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.23333.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 7 + CfrI YGGCCR 2 6 . . 3 6 + CviJI RGCY 4 4 . . 3 6 + HaeIII GGCC 4 4 . . 10 13 - MnlI CCTC 3 2 . . 14 19 + AclI AACGTT 15 17 . . 15 18 + TaiI ACGT 18 14 . . 15 18 + SetI ASST 18 14 . . 15 18 + MaeII ACGT 15 17 . . 18 23 + AhaIII* TTTAAA 20 20 . . 19 22 + MseI TTAA 19 21 . . 28 33 + AhaIII* TTTAAA 30 30 . . 29 32 + MseI TTAA 29 31 . . 34 37 - MnlI CCTC 27 26 . . 55 58 + DpnI GATC 56 56 . . 55 58 + BstKTI GATC 57 55 . . 55 58 + MboI GATC 54 58 . . 55 59 - BinI* GGATC 49 50 . . 84 98 + XcmI CCANNNNNNNNNTGG 91 90 . . 87 91 + TfiI GAWTC 87 90 . . 87 91 + HinfI GANTC 87 90 . . 90 93 + TaqI TCGA 90 92 . . 90 95 + Hpy178III* TCNNGA 91 93 . . 98 101 + Csp6I GTAC 98 100 . . 94 98 - BccI CCATC 88 89 . . 98 101 + RsaI GTAC 99 99 . . 119 124 + Hpy178III* TCNNGA 120 122 . . 127 131 + MboII GAAGA 139 138 . . 127 132 - Ksp632I* CTCTTC 122 125 . . 130 133 - MnlI CCTC 123 122 . . 144 147 + SetI ASST 147 143 . . 144 147 + CviJI RGCY 145 145 . . 144 147 + AluI AGCT 145 145 . . 145 149 + BlsI GCNGC 147 146 . . 145 149 + BisI GCNGC 146 147 . . 145 149 + TseI GCWGC 145 148 . . 145 149 - BbvI GCAGC 132 136 . . 152 160 + AlwNI CAGNNNCTG 157 154 . . 154 158 + TfiI GAWTC 154 157 . . 154 158 + HinfI GANTC 154 157 . . 157 161 + Hpy188I TCNGA 159 158 . . 169 172 + SetI ASST 172 168 . . 170 173 + MnlI CCTC 180 179 . . 173 176 + AciI CCGC 173 175 . . 172 177 - EciI GGCGGA 162 160 . . 183 186 + SetI ASST 186 182 . . 192 197 + MmeI TCCRAC 217 215 . . 217 221 + Tsp4CI* ACNGT 219 218 . . 220 224 + Tsp45I GTSAC 219 224 . . 220 224 + MaeIII GTNAC 219 224 . . 231 236 + BsgI GTGCAG 252 250 . . 232 235 + CviRI* TGCA 233 233 . . 235 238 + SetI ASST 238 234 . . 233 238 - BspMI ACCTGC 224 228 . . 240 245 + XhoII RGATCY 240 244 . . 240 245 + BglII AGATCT 240 244 . . 241 244 + DpnI GATC 242 242 . . 241 244 + BstKTI GATC 243 241 . . 241 244 + MboI GATC 240 244 . . 243 247 + Hpy188I TCNGA 245 244 . . 250 254 + Tsp4CI* ACNGT 252 251 . . 256 259 + Hin6I GCGC 256 258 . . 256 259 + GlaI GCGC 257 257 . . 256 266 + MwoI GCNNNNNNNGC 262 259 . . 256 259 + HhaI GCGC 258 256 . . 261 266 + NspI RCATGY 265 261 . . 262 265 + FatI CATG 261 265 . . 262 265 + NlaIII CATG 265 261 . . 262 265 + CviAII CATG 262 264 . . 265 275 + MwoI GCNNNNNNNGC 271 268 . . 273 276 + CviRI* TGCA 274 274 . . 298 302 + BlsI GCNGC 300 299 . . 298 302 + BisI GCNGC 299 300 . . 298 302 + TseI GCWGC 298 301 . . 298 302 - BbvI GCAGC 285 289 . . 299 304 - EcoP15I CAGCAG 271 273 . . 309 312 + FatI CATG 308 312 . . 309 312 + NlaIII CATG 312 308 . . 309 312 + CviAII CATG 309 311 . . 323 326 + CviJI RGCY 324 324 . . 332 337 + SfeI* CTRYAG 332 336 . . 336 339 + SetI ASST 339 335 . . 336 339 + CviJI RGCY 337 337 . . 336 339 + AluI AGCT 337 337 . . 340 343 + MseI TTAA 340 342 . . 342 345 + TspEI AATT 341 345 . . 350 353 + MnlI CCTC 360 359 . . 350 356 + SauI* CCTNAGG 351 354 . . 351 355 + DdeI CTNAG 351 354 . . 351 355 + BspCNI CTCAG 364 362 . . 351 355 + BseMII CTCAG 365 363 . . 367 371 + MaeIII GTNAC 366 371 . . 370 375 + Cfr10I RCCGGY 370 374 . . 370 375 + BetI* WCCGGW 370 374 . . 370 375 + AgeI ACCGGT 370 374 . . 371 374 + HpaII CCGG 371 373 . . 375 378 + CviRI* TGCA 376 376 . . 385 395 + BplI GAGNNNNNCTC 376 371 408 403 387 391 + FokI GGATG 400 404 . . 387 391 + BseGI GGATG 393 391 . . 385 388 - MnlI CCTC 378 377 . . 391 395 + HinfI GANTC 391 394 . . 391 395 - PleI GAGTC 385 386 . . 391 395 - MlyI GAGTC 385 385 . . 385 395 - BplI GAGNNNNNCTC 408 403 376 371 398 401 + SetI ASST 401 397 . . 398 401 + CviJI RGCY 399 399 . . 398 401 + AluI AGCT 399 399 . . 399 404 + Cac8I GCNNGC 401 401 . . 401 406 + BalI TGGCCA 403 403 . . 401 406 + CfrI YGGCCR 401 405 . . 402 405 + CviJI RGCY 403 403 . . 402 405 + HaeIII GGCC 403 403 . . 404 408 - BsrI ACTGG 404 402 . . 417 420 + CviRI* TGCA 418 418 . . 423 426 + TspEI AATT 422 426 . . 445 449 + BlsI GCNGC 447 446 . . 445 449 + BisI GCNGC 446 447 . . 445 449 + TseI GCWGC 445 448 . . 445 449 - BbvI GCAGC 432 436 . . 451 456 + ApoI RAATTY 451 455 . . 452 455 + TspEI AATT 451 455 . . 475 480 + SspI AATATT 477 477 . . 491 495 + Tsp45I GTSAC 490 495 . . 491 495 + MaeIII GTNAC 490 495 . . 494 497 + TaiI ACGT 497 493 . . 494 497 + SetI ASST 497 493 . . 494 497 + MaeII ACGT 494 496 . . 497 500 + MseI TTAA 497 499 . . 499 504 + ApoI RAATTY 499 503 . . 500 503 + TspEI AATT 499 503 . . 510 513 + TaiI ACGT 513 509 . . 510 513 + SetI ASST 513 509 . . 510 513 + MaeII ACGT 510 512 . . 513 518 + XbaI TCTAGA 513 517 . . 513 518 + Hpy178III* TCNNGA 514 516 . . 514 517 + MaeI CTAG 514 516 . . 517 521 - Hin4II* CCTTC 511 510 . . 526 531 - BsmI GAATGC 526 524 . . 535 538 + FatI CATG 534 538 . . 535 538 + NlaIII CATG 538 534 . . 535 538 + CviAII CATG 535 537 . . 539 542 + Csp6I GTAC 539 541 . . 539 542 + RsaI GTAC 540 540 . . 548 551 + MnlI CCTC 558 557 . . 546 551 - BseRI GAGGAG 537 535 . . 557 560 + CviJI RGCY 558 558 . . 562 571 + XmnI GAANNNNTTC 566 566 . . 563 566 + TspEI AATT 562 566 . . 571 575 + BssKI CCNGG 570 575 . . 571 575 + BseBI CCWGG 572 573 . . 571 575 + ScrFI CCNGG 572 573 . . 571 575 + EcoRII CCWGG 570 575 . . 579 582 + DpnI GATC 580 580 . . 579 582 + BstKTI GATC 581 579 . . 579 582 + MboI GATC 578 582 . . 582 587 + SfeI* CTRYAG 582 586 . . 591 595 + HphI GGTGA 603 602 . . 596 599 + CviJI RGCY 597 597 . . 603 606 + TspEI AATT 602 606 . . 613 616 + MseI TTAA 613 615 . . 615 618 + TspEI AATT 614 618 . . 625 630 + Hpy178III* TCNNGA 626 628 . . 628 640 - AloI GAACNNNNNNTCC 652 647 620 615 643 647 + BsrI ACTGG 648 646 . . 648 651 + CviRI* TGCA 649 649 . . 661 665 + MboII GAAGA 673 672 . . 665 670 + ApoI RAATTY 665 669 . . 666 669 + TspEI AATT 665 669 . . 674 679 + HindIII AAGCTT 674 678 . . 675 678 + SetI ASST 678 674 . . 675 678 + CviJI RGCY 676 676 . . 675 678 + AluI AGCT 676 676 . . 676 686 + MwoI GCNNNNNNNGC 682 679 . . 684 687 + SetI ASST 687 683 . . 684 687 + CviJI RGCY 685 685 . . 684 687 + AluI AGCT 685 685 . . 700 704 + DdeI CTNAG 700 703 . . 706 711 + ApoI RAATTY 706 710 . . 707 710 + TspEI AATT 706 710 . . #--------------------------------------- # # # AciI: # [CCGC] # 173 550 # # AclI: # [AACGTT] # 15 708 # # AgeI: # [ACCGGT] # 353 370 # # AhaIII*: # [TTTAAA] # 10 20 693 # # AloI: # [GAACNNNNNNTCC] # 32 71 620 # # AluI: # [AGCT] # 9 38 62 145 192 277 # # AlwNI: # [CAGNNNCTG] # 157 566 # # ApoI: # [RAATTY] # 17 41 48 166 451 # # BalI: # [TGGCCA] # 320 403 # # BbvI: # [GCAGC] # 132 147 153 291 # # BccI: # [CCATC] # 88 635 # # BetI*: # [WCCGGW] # 353 370 # # BglII: # [AGATCT] # 240 483 # # BinI*: # [GGATC] # 49 674 # # BisI: # [GCNGC] # 146 147 153 277 # # BlsI: # [GCNGC] # 147 147 153 276 # # BplI: # [GAGNNNNNCTC] # 0 0 32 315 376 # # BseBI: # [CCWGG] # 151 572 # # BseGI: # [GGATG] # 330 393 # # BseMII: # [CTCAG] # 358 365 # # BseRI: # [GAGGAG] # 186 537 # # BsgI: # [GTGCAG] # 252 471 # # BsmI: # [GAATGC] # 197 526 # # BspCNI: # [CTCAG] # 359 364 # # BspMI: # [ACCTGC] # 224 499 # # BsrI: # [ACTGG] # 75 244 404 # # BssKI: # [CCNGG] # 153 570 # # BstKTI: # [GATC] # 57 142 186 338 # # Cac8I: # [GCNNGC] # 322 401 # # Cfr10I: # [RCCGGY] # 353 370 # # CfrI: # [YGGCCR] # 2 322 399 # # Csp6I: # [GTAC] # 98 184 441 # # CviAII: # [CATG] # 47 188 226 262 # # CviJI: # [RGCY] # 4 4 9 13 38 39 # 62 79 141 155 179 # # CviRI*: # [TGCA] # 41 42 74 102 231 233 # # DdeI: # [CTNAG] # 23 349 351 # # DpnI: # [GATC] # 56 143 186 338 # # EciI: # [GGCGGA] # 162 561 # # EcoP15I: # [CAGCAG] # 271 452 # # EcoRII: # [CCWGG] # 153 570 # # FatI: # [CATG] # 47 189 226 261 # # FokI: # [GGATG] # 323 400 # # GlaI: # [GCGC] # 257 466 # # HaeIII: # [GGCC] # 4 320 399 # # HhaI: # [GCGC] # 258 465 # # Hin4II*: # [CCTTC] # 212 511 # # Hin6I: # [GCGC] # 256 467 # # HindIII: # [AAGCTT] # 49 674 # # HinfI: # [GANTC] # 67 87 237 332 # # HpaII: # [CCGG] # 352 371 # # HphI: # [GGTGA] # 120 603 # # Hpy178III*: # [TCNNGA] # 29 91 97 112 394 # # Hpy188I: # [TCNGA] # 86 159 478 # # Ksp632I*: # [CTCTTC] # 122 601 # # MaeI: # [CTAG] # 209 514 # # MaeII: # [ACGT] # 15 16 213 479 # # MaeIII: # [GTNAC] # 124 147 219 233 # # MboI: # [GATC] # 54 145 186 338 # # MboII: # [GAAGA] # 50 139 534 # # MlyI: # [GAGTC] # 338 385 # # MmeI: # [TCCRAC] # 217 506 # # MnlI: # [CCTC] # 3 18 24 57 96 165 # 180 180 # # MseI: # [TTAA] # 10 19 110 116 157 311 # # MwoI: # [GCNNNNNNNGC] # 9 41 262 411 # # NlaIII: # [CATG] # 47 185 226 265 # # NspI: # [RCATGY] # 265 458 # # PleI: # [GAGTC] # 338 385 # # RsaI: # [GTAC] # 99 183 441 # # SauI*: # [CCTNAGG] # 351 372 # # ScrFI: # [CCNGG] # 151 572 # # SetI: # [ASST] # 9 14 16 18 25 36 # 52 62 96 101 129 165 # # SfeI*: # [CTRYAG] # 141 250 332 # # SspI: # [AATATT] # 246 477 # # TaiI: # [ACGT] # 16 18 210 479 # # TaqI: # [TCGA] # 90 633 # # TfiI: # [GAWTC] # 67 87 569 # # TseI: # [GCWGC] # 145 147 153 278 # # Tsp45I: # [GTSAC] # 219 233 271 # # Tsp4CI*: # [ACNGT] # 33 219 471 # # TspEI: # [AATT] # 12 17 29 40 41 48 # 51 63 81 341 # # XbaI: # [TCTAGA] # 210 513 # # XcmI: # [CCANNNNNNNNNTGG] # 91 632 # # XhoII: # [RGATCY] # 240 483 # # XmnI: # [GAANNNNTTC] # 157 566 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 723 # Reported_sequences: 1 # Reported_hitcount: 179 #---------------------------------------