Restriction Fragments for YER145C-A



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The currently selected gene/sequence is :

YER145C-A on chromosome V from coordinates 462823 to 462386.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.10435.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 6 11 + SspI AATATT 8 8 . . 15 19 + TauI GCSGC 18 15 . . 15 19 + BlsI GCNGC 17 16 . . 15 19 + BisI GCNGC 16 17 . . 16 19 + AciI CCGC 16 18 . . 20 23 + MseI TTAA 20 22 . . 26 31 - BsmI GAATGC 26 24 . . 34 39 + DsaI* CCRYGG 34 38 . . 34 39 + StyI CCWWGG 34 38 . . 34 39 + NcoI CCATGG 34 38 . . 34 39 + SecI* CCNNGG 34 38 . . 35 38 + FatI CATG 34 38 . . 35 38 + NlaIII CATG 38 34 . . 35 38 + CviAII CATG 35 37 . . 41 45 + BlsI GCNGC 43 42 . . 41 45 + BisI GCNGC 42 43 . . 41 45 + TseI GCWGC 41 44 . . 43 46 + CviRI* TGCA 44 44 . . 41 45 - BbvI GCAGC 28 32 . . 71 74 + MnlI CCTC 81 80 . . 71 77 + SauI* CCTNAGG 72 75 . . 72 76 + DdeI CTNAG 72 75 . . 72 76 + BspCNI CTCAG 85 83 . . 72 76 + BseMII CTCAG 86 84 . . 73 78 + Hpy178III* TCNNGA 74 76 . . 76 80 + BinI* GGATC 84 85 . . 77 80 + DpnI GATC 78 78 . . 77 80 + BstKTI GATC 79 77 . . 77 80 + MboI GATC 76 80 . . 78 83 + ClaI ATCGAT 79 81 . . 79 82 + TaqI TCGA 79 81 . . 85 88 + CviJI RGCY 86 86 . . 91 94 + TaqI TCGA 91 93 . . 93 101 + Tth111I GACNNNGTC 96 97 . . 97 101 - HgaI GACGC 86 91 . . 100 104 - MboII GAAGA 92 91 . . 99 104 - BbvII* GAAGAC 92 96 . . 104 108 - BsrI ACTGG 104 102 . . 111 116 + AclI AACGTT 112 114 . . 112 115 + TaiI ACGT 115 111 . . 112 115 + SetI ASST 115 111 . . 112 115 + MaeII ACGT 112 114 . . 114 119 + Hpy166II GTNNAC 116 116 . . 114 119 + HindII GTYRAC 116 116 . . 117 125 + Tth111I GACNNNGTC 120 121 . . 117 128 + DrdI GACNNNNNNGTC 123 121 . . 125 129 + Hpy99I CGWCG 129 124 . . 127 130 + TaqI TCGA 127 129 . . 140 143 + TaiI ACGT 143 139 . . 140 143 + SetI ASST 143 139 . . 140 143 + MaeII ACGT 140 142 . . 142 147 + SduI GDGCHC 146 142 . . 142 147 + BseSI GKGCMC 146 142 . . 146 149 + MnlI CCTC 156 155 . . 148 151 + TaqI TCGA 148 150 . . 155 158 + FnuDII* CGCG 156 156 . . 156 159 - AciI CCGC 156 158 . . 161 164 + TaqI TCGA 161 163 . . 165 170 + PstI CTGCAG 169 165 . . 165 170 + SfeI* CTRYAG 165 169 . . 166 169 + CviRI* TGCA 167 167 . . 167 171 + BlsI GCNGC 169 168 . . 167 171 + BisI GCNGC 168 169 . . 167 171 + BbvI GCAGC 179 183 . . 167 171 + TseI GCWGC 167 170 . . 190 193 + MseI TTAA 190 192 . . 187 192 - TsoI TARCCA 177 175 . . 192 195 + TspEI AATT 191 195 . . 226 231 + BetI* WCCGGW 226 230 . . 227 230 + HpaII CCGG 227 229 . . 230 235 + Hpy166II GTNNAC 232 232 . . 230 235 + AccI GTMKAC 231 233 . . 233 237 + HinfI GANTC 233 236 . . 233 237 - PleI GAGTC 227 228 . . 233 237 - MlyI GAGTC 227 227 . . 243 246 + Hin6I GCGC 243 245 . . 243 246 + GlaI GCGC 244 244 . . 243 248 + Cac8I GCNNGC 245 245 . . 243 248 + BsePI GCGCGC 243 247 . . 243 246 + HhaI GCGC 245 243 . . 244 247 + FnuDII* CGCG 245 245 . . 245 248 + Hin6I GCGC 245 247 . . 245 248 + GlaI GCGC 246 246 . . 245 248 + HhaI GCGC 247 245 . . 267 272 + Hpy166II GTNNAC 269 269 . . 267 272 + SduI GDGCHC 271 267 . . 267 272 + BseSI GKGCMC 271 267 . . 267 272 + ApaLI GTGCAC 267 271 . . 267 272 + HgiAI* GWGCWC 271 267 . . 268 271 + CviRI* TGCA 269 269 . . 270 275 + BsaAI YACGTR 272 272 . . 270 275 + PmaCI CACGTG 272 272 . . 271 274 + TaiI ACGT 274 270 . . 271 274 + SetI ASST 274 270 . . 271 274 + MaeII ACGT 271 273 . . 271 276 + AflIII ACRYGT 271 275 . . 275 280 + Hpy166II GTNNAC 277 277 . . 275 280 + BssNAI GTATAC 277 277 . . 275 280 + AccI GTMKAC 276 278 . . 297 307 + Hin4I GAYNNNNNVTC 288 283 320 315 299 303 + BccI CCATC 307 308 . . 295 299 - BsmAI GTCTC 289 293 . . 295 300 - Eco31I GGTCTC 289 293 . . 297 307 - Hin4I GAYNNNNNVTC 320 315 288 283 307 310 + AciI CCGC 307 309 . . 309 319 + MwoI GCNNNNNNNGC 315 312 . . 310 315 + StyI CCWWGG 310 314 . . 310 315 + SecI* CCNNGG 310 314 . . 306 311 - EciI GGCGGA 296 294 . . 312 322 + FalI AAGNNNNNCTT 303 298 335 330 317 320 + CviJI RGCY 318 318 . . 318 328 + MwoI GCNNNNNNNGC 324 321 . . 312 322 - FalI AAGNNNNNCTT 335 330 303 298 381 385 + BssKI CCNGG 380 385 . . 381 385 + ScrFI CCNGG 382 383 . . 381 385 + CauII* CCSGG 382 383 . . 382 385 + HpaII CCGG 382 384 . . 384 388 + FokI GGATG 397 401 . . 384 388 + BseGI GGATG 390 388 . . 387 390 + CviRI* TGCA 388 388 . . 385 389 - SfaNI GCATC 375 379 . . 400 405 + BmeT110I CYCGRG 401 403 . . 400 405 + AvaI CYCGRG 400 404 . . 405 409 + SfaNI GCATC 414 418 . . 409 413 + BssKI CCNGG 408 413 . . 409 412 + HpaII CCGG 409 411 . . 409 413 + ScrFI CCNGG 410 411 . . 409 413 + CauII* CCSGG 410 411 . . 406 410 - FokI GGATG 392 396 . . 406 410 - BseGI GGATG 405 403 . . 427 432 + NlaIV GGNNCC 429 429 . . 428 432 + BmgT120I GGNCC 429 430 . . 428 432 + AvaII GGWCC 428 431 . . 428 432 + AsuI* GGNCC 428 431 . . 431 434 + HpaII CCGG 431 433 . . 433 436 + CviJI RGCY 434 434 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 45 162 231 # # AciI: # [CCGC] # 16 131 140 151 # # AclI: # [AACGTT] # 112 326 # # AflIII: # [ACRYGT] # 167 271 # # ApaLI: # [GTGCAC] # 171 267 # # AsuI*: # [GGNCC] # 10 428 # # AvaI: # [CYCGRG] # 38 400 # # AvaII: # [GGWCC] # 10 428 # # BbvI: # [GCAGC] # 28 151 259 # # BbvII*: # [GAAGAC] # 92 346 # # BccI: # [CCATC] # 131 307 # # BetI*: # [WCCGGW] # 212 226 # # BinI*: # [GGATC] # 84 354 # # BisI: # [GCNGC] # 16 26 126 270 # # BlsI: # [GCNGC] # 17 26 126 269 # # BmeT110I: # [CYCGRG] # 37 401 # # BmgT120I: # [GGNCC] # 9 429 # # BsaAI: # [YACGTR] # 166 272 # # BseGI: # [GGATG] # 15 33 390 # # BseMII: # [CTCAG] # 86 352 # # BsePI: # [GCGCGC] # 195 243 # # BseSI: # [GKGCMC] # 125 146 167 # # BsmAI: # [GTCTC] # 149 289 # # BsmI: # [GAATGC] # 26 412 # # BspCNI: # [CTCAG] # 85 353 # # BsrI: # [ACTGG] # 104 334 # # BssKI: # [CCNGG] # 28 30 380 # # BssNAI: # [GTATAC] # 161 277 # # BstKTI: # [GATC] # 79 359 # # Cac8I: # [GCNNGC] # 193 245 # # CauII*: # [CCSGG] # 28 28 382 # # ClaI: # [ATCGAT] # 79 359 # # CviAII: # [CATG] # 35 403 # # CviJI: # [RGCY] # 4 86 116 232 # # CviRI*: # [TGCA] # 44 50 102 119 123 # # DdeI: # [CTNAG] # 72 366 # # DpnI: # [GATC] # 78 360 # # DrdI: # [GACNNNNNNGTC] # 123 315 # # DsaI*: # [CCRYGG] # 34 404 # # EciI: # [GGCGGA] # 142 296 # # Eco31I: # [GGTCTC] # 149 289 # # FalI: # [AAGNNNNNCTT] # 0 0 32 103 303 # # FatI: # [CATG] # 34 404 # # FnuDII*: # [CGCG] # 89 156 193 # # FokI: # [GGATG] # 5 41 392 # # GlaI: # [GCGC] # 2 192 244 # # HgaI: # [GACGC] # 86 352 # # HgiAI*: # [GWGCWC] # 167 271 # # HhaI: # [GCGC] # 2 191 245 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 118 288 # # Hin6I: # [GCGC] # 2 193 243 # # HindII: # [GTYRAC] # 116 322 # # HinfI: # [GANTC] # 205 233 # # HpaII: # [CCGG] # 7 22 27 155 227 # # Hpy166II: # [GTNNAC] # 8 37 116 116 161 # # Hpy178III*: # [TCNNGA] # 74 364 # # Hpy99I: # [CGWCG] # 129 309 # # MaeII: # [ACGT] # 28 112 131 167 # # MboI: # [GATC] # 76 362 # # MboII: # [GAAGA] # 92 346 # # MlyI: # [GAGTC] # 211 227 # # MnlI: # [CCTC] # 75 81 282 # # MseI: # [TTAA] # 20 170 248 # # MwoI: # [GCNNNNNNNGC] # 9 114 315 # # NcoI: # [CCATGG] # 34 404 # # NlaIII: # [CATG] # 38 400 # # NlaIV: # [GGNNCC] # 9 429 # # PleI: # [GAGTC] # 211 227 # # PmaCI: # [CACGTG] # 166 272 # # PstI: # [CTGCAG] # 169 269 # # SauI*: # [CCTNAGG] # 72 366 # # ScrFI: # [CCNGG] # 28 28 382 # # SduI: # [GDGCHC] # 125 146 167 # # SecI*: # [CCNNGG] # 34 128 276 # # SetI: # [ASST] # 28 115 131 164 # # SfaNI: # [GCATC] # 24 39 375 # # SfeI*: # [CTRYAG] # 165 273 # # SspI: # [AATATT] # 8 430 # # StyI: # [CCWWGG] # 34 128 276 # # TaiI: # [ACGT] # 28 115 131 164 # # TaqI: # [TCGA] # 12 13 21 36 79 277 # # TauI: # [GCSGC] # 18 420 # # TseI: # [GCWGC] # 41 126 271 # # TsoI: # [TARCCA] # 177 261 # # TspEI: # [AATT] # 191 247 # # Tth111I: # [GACNNNGTC] # 24 96 318 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 438 # Reported_sequences: 1 # Reported_hitcount: 136 #---------------------------------------