Restriction Fragments for MAM1/YER106W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

MAM1/YER106W on chromosome V from coordinates 372326 to 373234.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.13295.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 14 23 + XmnI GAANNNNTTC 18 18 . . 18 21 + TspEI AATT 17 21 . . 46 51 + ApoI RAATTY 46 50 . . 47 50 + TspEI AATT 46 50 . . 73 78 + Hpy178III* TCNNGA 74 76 . . 77 81 + TfiI GAWTC 77 80 . . 77 81 + HinfI GANTC 77 80 . . 80 85 + Hpy178III* TCNNGA 81 83 . . 81 86 + SmlI CTYRAG 81 85 . . 81 86 + Bce83I* CTTGAG 102 100 . . 90 94 + MboII GAAGA 102 101 . . 93 98 + EcoRV GATATC 95 95 . . 106 109 + CviJI RGCY 107 107 . . 123 127 + MboII GAAGA 135 134 . . 126 130 + MboII GAAGA 138 137 . . 142 148 + TspRI CASTGNN 148 139 . . 143 147 + Tsp4CI* ACNGT 145 144 . . 151 154 + TaqI TCGA 151 153 . . 193 198 + BetI* WCCGGW 193 197 . . 194 197 + HpaII CCGG 194 196 . . 204 207 + CviRI* TGCA 205 205 . . 205 209 + BlsI GCNGC 207 206 . . 205 209 + BisI GCNGC 206 207 . . 205 209 + BbvI GCAGC 217 221 . . 205 209 + TseI GCWGC 205 208 . . 214 218 + MboII GAAGA 226 225 . . 214 219 + BbvII* GAAGAC 221 225 . . 228 232 + BlsI GCNGC 230 229 . . 228 232 + BisI GCNGC 229 230 . . 228 232 + BbvI GCAGC 240 244 . . 228 232 + TseI GCWGC 228 231 . . 254 258 + TspDTI ATGAA 269 267 . . 256 260 + MboII GAAGA 268 267 . . 265 269 + TspGWI ACGGA 280 278 . . 286 289 + TspEI AATT 285 289 . . 297 301 + MaeIII GTNAC 296 301 . . 306 315 + XmnI GAANNNNTTC 310 310 . . 308 313 + ApoI RAATTY 308 312 . . 309 312 + TspEI AATT 308 312 . . 314 317 + TaqI TCGA 314 316 . . 326 329 + MseI TTAA 326 328 . . 342 347 + BclI TGATCA 342 346 . . 343 346 + DpnI GATC 344 344 . . 343 346 + BstKTI GATC 345 343 . . 343 346 + MboI GATC 342 346 . . 371 375 + MboII GAAGA 383 382 . . 376 381 + EcoRV GATATC 378 378 . . 371 376 - Ksp632I* CTCTTC 366 369 . . 388 393 + Hpy178III* TCNNGA 389 391 . . 398 403 + NlaIV GGNNCC 400 400 . . 411 416 - MmeI TCCRAC 392 390 . . 428 432 + BsrI ACTGG 433 431 . . 434 438 + TspDTI ATGAA 449 447 . . 444 447 + AciI CCGC 444 446 . . 446 456 + MwoI GCNNNNNNNGC 452 449 . . 443 448 - EciI GGCGGA 433 431 . . 448 452 + DdeI CTNAG 448 451 . . 476 479 + AciI CCGC 476 478 . . 479 484 + MfeI CAATTG 479 483 . . 480 483 + TspEI AATT 479 483 . . 487 491 + PleI GAGTC 495 496 . . 487 491 + MlyI GAGTC 496 496 . . 487 491 + HinfI GANTC 487 490 . . 505 510 + SspI AATATT 507 507 . . 515 518 + MseI TTAA 515 517 . . 536 540 + TspDTI ATGAA 551 549 . . 538 547 + XmnI GAANNNNTTC 542 542 . . 539 544 + SspI AATATT 541 541 . . 557 560 + TspEI AATT 556 560 . . 574 579 + SspI AATATT 576 576 . . 603 607 - Hin4II* CCTTC 597 596 . . 616 620 + MboII GAAGA 628 627 . . 616 621 + BbvII* GAAGAC 623 627 . . 622 625 + MseI TTAA 622 624 . . 626 631 + MfeI CAATTG 626 630 . . 627 630 + TspEI AATT 626 630 . . 633 636 + TspEI AATT 632 636 . . 651 661 + Hin4I GAYNNNNNVTC 642 637 674 669 649 653 - BsmAI GTCTC 643 647 . . 657 661 + BccI CCATC 665 666 . . 651 661 - Hin4I GAYNNNNNVTC 674 669 642 637 678 682 + TauI GCSGC 681 678 . . 678 682 + BlsI GCNGC 680 679 . . 678 682 + BisI GCNGC 679 680 . . 679 682 + AciI CCGC 679 681 . . 689 694 + Hpy178III* TCNNGA 690 692 . . 705 709 + FokI GGATG 718 722 . . 705 709 + BseGI GGATG 711 709 . . 703 706 - MnlI CCTC 696 695 . . 707 711 + TspDTI ATGAA 722 720 . . 711 714 + TspEI AATT 710 714 . . 714 717 + CviRI* TGCA 715 715 . . 720 731 + BdaI TGANNNNNNTCA 709 707 743 741 730 739 + MslI CAYNNNNRTG 734 734 . . 720 731 - BdaI TGANNNNNNTCA 743 741 709 707 728 732 - TspDTI ATGAA 718 716 . . 735 739 + FokI GGATG 748 752 . . 735 739 + BseGI GGATG 741 739 . . 738 741 + CviRI* TGCA 739 739 . . 736 740 - SfaNI GCATC 726 730 . . 746 749 + SetI ASST 749 745 . . 763 768 + HaeII RGCGCY 767 763 . . 764 767 + Hin6I GCGC 764 766 . . 764 767 + GlaI GCGC 765 765 . . 764 767 + HhaI GCGC 766 764 . . 772 776 + Hpy188I TCNGA 774 773 . . 787 790 + SetI ASST 790 786 . . 787 790 + CviJI RGCY 788 788 . . 787 790 + AluI AGCT 788 788 . . 788 794 + SapI GCTCTTC 795 798 . . 789 794 + Ksp632I* CTCTTC 795 798 . . 790 794 - MboII GAAGA 782 781 . . 793 797 - HphI GGTGA 785 784 . . 810 813 + TspEI AATT 809 813 . . 812 815 + MseI TTAA 812 814 . . 829 832 + SetI ASST 832 828 . . 830 835 + StyI CCWWGG 830 834 . . 830 835 + SecI* CCNNGG 830 834 . . 835 844 + XmnI GAANNNNTTC 839 839 . . 840 845 + NlaIV GGNNCC 842 842 . . 864 867 + DpnI GATC 865 865 . . 864 867 + BstKTI GATC 866 864 . . 864 867 + MboI GATC 863 867 . . 870 873 + SetI ASST 873 869 . . 887 890 + SetI ASST 890 886 . . 887 890 + CviJI RGCY 888 888 . . 887 890 + AluI AGCT 888 888 . . 892 901 + MslI CAYNNNNRTG 896 896 . . 904 907 + TspEI AATT 903 907 . . 906 909 + MseI TTAA 906 908 . . #--------------------------------------- # # # AciI: # [CCGC] # 32 203 230 444 # # AluI: # [AGCT] # 21 100 788 # # ApoI: # [RAATTY] # 46 262 601 # # BbvI: # [GCAGC] # 23 217 669 # # BbvII*: # [GAAGAC] # 221 286 402 # # BccI: # [CCATC] # 244 665 # # Bce83I*: # [CTTGAG] # 102 807 # # BclI: # [TGATCA] # 342 567 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 166 709 # # BetI*: # [WCCGGW] # 193 716 # # BisI: # [GCNGC] # 23 206 230 450 # # BlsI: # [GCNGC] # 23 207 229 450 # # BseGI: # [GGATG] # 30 168 711 # # BsmAI: # [GTCTC] # 266 643 # # BsrI: # [ACTGG] # 433 476 # # BstKTI: # [GATC] # 43 345 521 # # CviJI: # [RGCY] # 21 100 107 681 # # CviRI*: # [TGCA] # 24 170 205 510 # # DdeI: # [CTNAG] # 448 461 # # DpnI: # [GATC] # 44 344 521 # # EciI: # [GGCGGA] # 433 476 # # EcoRV: # [GATATC] # 95 283 531 # # FokI: # [GGATG] # 30 161 718 # # GlaI: # [GCGC] # 144 765 # # HaeII: # [RGCGCY] # 142 767 # # HhaI: # [GCGC] # 143 766 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 235 642 # # Hin4II*: # [CCTTC] # 312 597 # # Hin6I: # [GCGC] # 145 764 # # HinfI: # [GANTC] # 77 410 422 # # HpaII: # [CCGG] # 194 715 # # HphI: # [GGTGA] # 124 785 # # Hpy178III*: # [TCNNGA] # 7 74 219 301 308 # # Hpy188I: # [TCNGA] # 135 774 # # Ksp632I*: # [CTCTTC] # 114 366 429 # # MaeIII: # [GTNAC] # 296 613 # # MboI: # [GATC] # 46 342 521 # # MboII: # [GAAGA] # 3 33 42 88 102 115 # 127 154 245 # # MfeI: # [CAATTG] # 147 283 479 # # MlyI: # [GAGTC] # 413 496 # # MmeI: # [TCCRAC] # 392 517 # # MnlI: # [CCTC] # 213 696 # # MseI: # [TTAA] # 3 94 107 189 190 326 # # MslI: # [CAYNNNNRTG] # 13 162 734 # # MwoI: # [GCNNNNNNNGC] # 452 457 # # NlaIV: # [GGNNCC] # 67 400 442 # # PleI: # [GAGTC] # 414 495 # # SapI: # [GCTCTTC] # 114 795 # # SecI*: # [CCNNGG] # 79 830 # # SetI: # [ASST] # 17 19 41 41 42 749 # # SfaNI: # [GCATC] # 183 726 # # SmlI: # [CTYRAG] # 81 828 # # SspI: # [AATATT] # 34 35 333 507 # # StyI: # [CCWWGG] # 79 830 # # TaqI: # [TCGA] # 151 163 595 # # TauI: # [GCSGC] # 228 681 # # TfiI: # [GAWTC] # 77 832 # # TseI: # [GCWGC] # 23 205 681 # # Tsp4CI*: # [ACNGT] # 145 764 # # TspDTI: # [ATGAA] # 4 102 167 180 187 269 # # TspEI: # [AATT] # 6 6 17 23 29 70 # 77 78 94 99 171 239 # # TspGWI: # [ACGGA] # 280 629 # # TspRI: # [CASTGNN] # 148 761 # # XmnI: # [GAANNNNTTC] # 18 70 232 292 297 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 909 # Reported_sequences: 1 # Reported_hitcount: 130 #---------------------------------------