Restriction Fragments for RPS24A/YER074W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

RPS24A/YER074W on chromosome V from coordinates 306323 to 307196.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.12954.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 9 12 + MseI TTAA 9 11 . . 16 21 + SduI GDGCHC 20 16 . . 16 21 + HgiAI* GWGCWC 20 16 . . 19 23 + DdeI CTNAG 19 22 . . 19 23 + BspCNI CTCAG 32 30 . . 19 23 + BseMII CTCAG 33 31 . . 20 24 + Hpy188I TCNGA 22 21 . . 24 28 + TspDTI ATGAA 39 37 . . 33 38 + ZraI GACGTC 35 35 . . 33 38 + AcyI GRCGYC 34 36 . . 33 38 + AatII GACGTC 37 33 . . 34 37 + TaiI ACGT 37 33 . . 34 37 + SetI ASST 37 33 . . 34 37 + MaeII ACGT 34 36 . . 36 40 - BslFI GGGAC 21 25 . . 47 50 + FatI CATG 46 50 . . 47 50 + NlaIII CATG 50 46 . . 47 50 + CviAII CATG 47 49 . . 51 54 + MseI TTAA 51 53 . . 61 64 + FatI CATG 60 64 . . 61 64 + NlaIII CATG 64 60 . . 61 64 + CviAII CATG 61 63 . . 86 89 + SetI ASST 89 85 . . 87 92 + KpnI GGTACC 91 87 . . 87 92 + NlaIV GGNNCC 89 89 . . 87 92 + HgiCI* GGYRCC 87 91 . . 87 92 + Acc65I GGTACC 87 91 . . 88 91 + Csp6I GTAC 88 90 . . 88 91 + RsaI GTAC 89 89 . . 115 119 + MboII GAAGA 127 126 . . 127 130 + CviRI* TGCA 128 128 . . 130 135 + ApoI RAATTY 130 134 . . 131 134 + TspEI AATT 130 134 . . 134 137 + MseI TTAA 134 136 . . 137 140 + SetI ASST 140 136 . . 144 148 + MaeIII GTNAC 143 148 . . 159 162 + CviJI RGCY 160 160 . . 158 163 - TsoI TARCCA 148 146 . . 168 173 + VspI ATTAAT 169 171 . . 169 172 + MseI TTAA 169 171 . . 202 206 - TspGWI ACGGA 192 190 . . 212 215 + TspEI AATT 211 215 . . 218 221 + MseI TTAA 218 220 . . 215 220 - TsoI TARCCA 205 203 . . 220 225 + ApoI RAATTY 220 224 . . 221 224 + TspEI AATT 220 224 . . 224 229 + PsiI TTATAA 226 226 . . 233 238 + ApoI RAATTY 233 237 . . 234 237 + TspEI AATT 233 237 . . 236 241 + AhaIII* TTTAAA 238 238 . . 237 240 + MseI TTAA 237 239 . . 245 248 + TaqI TCGA 245 247 . . 251 254 + SetI ASST 254 250 . . 251 254 + CviJI RGCY 252 252 . . 251 254 + AluI AGCT 252 252 . . 262 267 + SspI AATATT 264 264 . . 272 276 + FauI CCCGC 280 282 . . 273 276 + AciI CCGC 273 275 . . 278 281 + MseI TTAA 278 280 . . 301 306 + EcoRV GATATC 303 303 . . 306 309 + MaeI CTAG 306 308 . . 308 311 + SetI ASST 311 307 . . 308 311 + CviJI RGCY 309 309 . . 308 311 + AluI AGCT 309 309 . . 311 316 + TsoI TARCCA 327 325 . . 322 327 + SfeI* CTRYAG 322 326 . . 327 332 + NlaIV GGNNCC 329 329 . . 330 333 + SetI ASST 333 329 . . 354 359 + Hpy178III* TCNNGA 355 357 . . 354 359 + BspHI TCATGA 354 358 . . 355 358 + FatI CATG 354 358 . . 355 358 + NlaIII CATG 358 354 . . 355 358 + CviAII CATG 355 357 . . 356 360 + TspDTI ATGAA 371 369 . . 362 366 + BsrI ACTGG 367 365 . . 369 374 + XhoII RGATCY 369 373 . . 369 373 + BinI* GGATC 377 378 . . 366 370 - Hin4II* CCTTC 360 359 . . 370 373 + DpnI GATC 371 371 . . 370 373 + BstKTI GATC 372 370 . . 370 373 + MboI GATC 369 373 . . 381 385 + HgaI GACGC 390 395 . . 381 386 + AcyI GRCGYC 382 384 . . 402 412 + BsiYI* CCNNNNNNNGG 408 405 . . 407 413 + TspRI CASTGNN 413 404 . . 408 412 + BsrI ACTGG 413 411 . . 411 416 + NlaIV GGNNCC 413 413 . . 412 424 + PsrI GAACNNNNNNTAC 404 399 436 431 414 417 + SetI ASST 417 413 . . 429 432 + TspEI AATT 428 432 . . 433 438 + AhaIII* TTTAAA 435 435 . . 434 437 + MseI TTAA 434 436 . . 449 452 + SetI ASST 452 448 . . 454 457 + TspEI AATT 453 457 . . 465 469 + DdeI CTNAG 465 468 . . 465 469 + BspCNI CTCAG 478 476 . . 465 469 + BseMII CTCAG 479 477 . . 470 474 + Hpy188I TCNGA 472 471 . . 473 477 + HgaI GACGC 482 487 . . 479 483 + MaeIII GTNAC 478 483 . . 489 492 + Csp6I GTAC 489 491 . . 489 492 + RsaI GTAC 490 490 . . 486 490 - TspGWI ACGGA 476 474 . . 492 495 + MaeI CTAG 492 494 . . 498 501 + SetI ASST 501 497 . . 506 511 + MmeI TCCRAC 531 529 . . 507 521 + XcmI CCANNNNNNNNNTGG 514 513 . . 511 521 + BsiYI* CCNNNNNNNGG 517 514 . . 519 524 + BalI TGGCCA 521 521 . . 519 524 + CfrI YGGCCR 519 523 . . 520 523 + CviJI RGCY 521 521 . . 520 523 + HaeIII GGCC 521 521 . . 531 534 + TspEI AATT 530 534 . . 535 539 + Hpy99I CGWCG 539 534 . . 538 545 + SgrDI CGTCGACG 539 543 . . 538 542 + Hpy99I CGWCG 542 537 . . 539 544 + SalI GTCGAC 539 543 . . 539 544 + Hpy166II GTNNAC 541 541 . . 539 544 + HindII GTYRAC 541 541 . . 539 544 + AccI GTMKAC 540 542 . . 540 543 + TaqI TCGA 540 542 . . 541 545 + Hpy99I CGWCG 545 540 . . 542 547 + ZraI GACGTC 544 544 . . 542 547 + AcyI GRCGYC 543 545 . . 542 547 + AatII GACGTC 546 542 . . 543 546 + TaiI ACGT 546 542 . . 543 546 + SetI ASST 546 542 . . 543 546 + MaeII ACGT 543 545 . . 549 552 + CviRI* TGCA 550 550 . . 551 556 + TaqII CACCCA 567 565 . . 562 565 + SetI ASST 565 561 . . 562 565 + CviJI RGCY 563 563 . . 562 565 + AluI AGCT 563 563 . . 569 573 + BsmAI GTCTC 574 578 . . 561 571 - Hin4I GAYNNNNNVTC 584 579 552 547 573 578 + StyI CCWWGG 573 577 . . 573 578 + SecI* CCNNGG 573 577 . . 577 581 + FokI GGATG 590 594 . . 577 581 + BseGI GGATG 583 581 . . 579 583 + TspDTI ATGAA 594 592 . . 582 585 + TspEI AATT 581 585 . . 594 597 + TspEI AATT 593 597 . . 598 601 + SetI ASST 601 597 . . 598 601 + CviJI RGCY 599 599 . . 598 601 + AluI AGCT 599 599 . . 600 605 + Eco57MI CTGRAG 621 619 . . 600 605 + Eco57I CTGAAG 621 619 . . 605 610 + Hpy166II GTNNAC 607 607 . . 605 610 + AccI GTMKAC 606 608 . . 613 616 + CviJI RGCY 614 614 . . 623 633 + Hin4I GAYNNNNNVTC 614 609 646 641 623 627 + HgaI GACGC 632 637 . . 623 631 + Tth111I GACNNNGTC 626 627 . . 624 634 + BsaXI ACNNNNNCTCC 614 611 644 641 629 633 + BsmAI GTCTC 634 638 . . 632 636 - TspGWI ACGGA 622 620 . . 645 649 + Hpy188I TCNGA 647 646 . . 652 666 + XcmI CCANNNNNNNNNTGG 659 658 . . 652 663 + BstXI CCANNNNNNTGG 659 655 . . 654 657 + TspEI AATT 653 657 . . 689 694 + Hpy166II GTNNAC 691 691 . . 689 694 + AccI GTMKAC 690 692 . . 709 712 + SetI ASST 712 708 . . 709 712 + CviJI RGCY 710 710 . . 709 712 + AluI AGCT 710 710 . . 711 715 + DdeI CTNAG 711 714 . . 719 724 + AsuII TTCGAA 720 722 . . 720 723 + TaqI TCGA 720 722 . . 741 745 + Hpy188I TCNGA 743 742 . . 747 751 + Tsp4CI* ACNGT 749 748 . . 754 757 + CviJI RGCY 755 755 . . 762 765 + SetI ASST 765 761 . . 772 775 + CviJI RGCY 773 773 . . 776 781 + Hpy178III* TCNNGA 777 779 . . 799 803 + MboII GAAGA 811 810 . . 807 811 - BsmAI GTCTC 801 805 . . 812 822 + FalI AAGNNNNNCTT 803 798 835 830 814 818 + MboII GAAGA 826 825 . . 814 823 + XmnI GAANNNNTTC 818 818 . . 816 821 + XhoII RGATCY 816 820 . . 816 821 + BglII AGATCT 816 820 . . 817 820 + DpnI GATC 818 818 . . 817 820 + BstKTI GATC 819 817 . . 817 820 + MboI GATC 816 820 . . 812 822 - FalI AAGNNNNNCTT 835 830 803 798 825 828 + Csp6I GTAC 825 827 . . 825 828 + RsaI GTAC 826 826 . . 827 831 + BsrI ACTGG 832 830 . . 841 844 + CviJI RGCY 842 842 . . 843 847 + DdeI CTNAG 843 846 . . 840 845 - TsoI TARCCA 830 828 . . 849 852 + SetI ASST 852 848 . . 847 851 - Hin4II* CCTTC 841 840 . . 857 861 + Hpy99I CGWCG 861 856 . . 861 865 + MaeIII GTNAC 860 865 . . 871 874 + MseI TTAA 871 873 . . #--------------------------------------- # # # AatII: # [GACGTC] # 37 328 509 # # Acc65I: # [GGTACC] # 87 787 # # AccI: # [GTMKAC] # 66 84 184 540 # # AciI: # [CCGC] # 273 601 # # AcyI: # [GRCGYC] # 34 161 331 348 # # AhaIII*: # [TTTAAA] # 197 238 439 # # AluI: # [AGCT] # 36 57 111 164 252 254 # # ApoI: # [RAATTY] # 13 90 130 641 # # AsuII: # [TTCGAA] # 154 720 # # BalI: # [TGGCCA] # 353 521 # # BglII: # [AGATCT] # 58 816 # # BinI*: # [GGATC] # 377 497 # # BsaXI: # [ACNNNNNCTCC] # 30 230 614 # # BseGI: # [GGATG] # 291 583 # # BseMII: # [CTCAG] # 33 395 446 # # BsiYI*: # [CCNNNNNNNGG] # 109 357 408 # # BslFI: # [GGGAC] # 21 853 # # BsmAI: # [GTCTC] # 60 73 167 574 # # BspCNI: # [CTCAG] # 32 396 446 # # BspHI: # [TCATGA] # 354 520 # # BsrI: # [ACTGG] # 42 46 367 419 # # BstKTI: # [GATC] # 55 372 447 # # BstXI: # [CCANNNNNNTGG] # 215 659 # # CfrI: # [YGGCCR] # 355 519 # # Csp6I: # [GTAC] # 49 88 336 401 # # CviAII: # [CATG] # 14 47 294 519 # # CviJI: # [RGCY] # 15 18 32 36 42 45 # 57 69 92 96 160 212 # # CviRI*: # [TGCA] # 128 324 422 # # DdeI: # [CTNAG] # 19 31 132 246 446 # # DpnI: # [GATC] # 56 371 447 # # Eco57I: # [CTGAAG] # 253 621 # # Eco57MI: # [CTGRAG] # 253 621 # # EcoRV: # [GATATC] # 303 571 # # FalI: # [AAGNNNNNCTT] # 0 0 32 39 803 # # FatI: # [CATG] # 14 46 294 520 # # FauI: # [CCCGC] # 280 594 # # FokI: # [GGATG] # 284 590 # # HaeIII: # [GGCC] # 353 521 # # HgaI: # [GACGC] # 92 150 242 390 # # HgiAI*: # [GWGCWC] # 20 854 # # HgiCI*: # [GGYRCC] # 87 787 # # Hin4I: # [GAYNNNNNVTC] # 30 32 32 228 552 # # Hin4II*: # [CCTTC] # 33 360 481 # # HindII: # [GTYRAC] # 333 541 # # Hpy166II: # [GTNNAC] # 66 84 183 541 # # Hpy178III*: # [TCNNGA] # 97 355 422 # # Hpy188I: # [TCNGA] # 22 96 131 175 450 # # Hpy99I: # [CGWCG] # 3 3 13 316 539 # # KpnI: # [GGTACC] # 91 783 # # MaeI: # [CTAG] # 186 306 382 # # MaeII: # [ACGT] # 34 331 509 # # MaeIII: # [GTNAC] # 14 143 335 382 # # MboI: # [GATC] # 58 369 447 # # MboII: # [GAAGA] # 15 48 127 684 # # MmeI: # [TCCRAC] # 343 531 # # MseI: # [TTAA] # 3 9 19 35 41 42 # 49 83 156 437 # # NlaIII: # [CATG] # 14 50 294 516 # # NlaIV: # [GGNNCC] # 84 89 240 461 # # PsiI: # [TTATAA] # 226 648 # # PsrI: # [GAACNNNNNNTAC] # 32 404 438 # # RsaI: # [GTAC] # 48 89 336 401 # # SalI: # [GTCGAC] # 335 539 # # SduI: # [GDGCHC] # 20 854 # # SecI*: # [CCNNGG] # 301 573 # # SetI: # [ASST] # 19 22 22 35 36 37 # 45 49 51 52 53 57 # 84 87 111 114 # # SfeI*: # [CTRYAG] # 322 552 # # SgrDI: # [CGTCGACG] # 335 539 # # SspI: # [AATATT] # 264 610 # # StyI: # [CCWWGG] # 301 573 # # TaiI: # [ACGT] # 37 328 509 # # TaqI: # [TCGA] # 154 180 245 295 # # TaqII: # [CACCCA] # 307 567 # # TsoI: # [TARCCA] # 44 57 122 148 503 # # Tsp4CI*: # [ACNGT] # 125 749 # # TspDTI: # [ATGAA] # 39 223 280 332 # # TspEI: # [AATT] # 9 12 13 25 51 60 # 77 81 130 195 221 # # TspGWI: # [ACGGA] # 146 192 252 284 # # TspRI: # [CASTGNN] # 413 461 # # Tth111I: # [GACNNNGTC] # 248 626 # # VspI: # [ATTAAT] # 169 705 # # XcmI: # [CCANNNNNNNNNTGG] # 145 215 514 # # XhoII: # [RGATCY] # 58 369 447 # # XmnI: # [GAANNNNTTC] # 56 818 # # ZraI: # [GACGTC] # 35 330 509 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 874 # Reported_sequences: 1 # Reported_hitcount: 196 #---------------------------------------