Restriction Fragments for VTC1/YER072W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

VTC1/YER072W on chromosome V from coordinates 302806 to 303195.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.5414.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 30 33 + SetI ASST 33 29 . . 31 35 + BssKI CCNGG 30 35 . . 31 35 + BseBI CCWGG 32 33 . . 31 36 + SecI* CCNNGG 31 35 . . 31 35 + ScrFI CCNGG 32 33 . . 31 35 + EcoRII CCWGG 30 35 . . 42 45 + DpnI GATC 43 43 . . 42 45 + BstKTI GATC 44 42 . . 42 45 + MboI GATC 41 45 . . 56 61 + BsiI* CACGAG 56 60 . . 53 64 - CspCI CAANNNNNGTGG 77 75 42 40 65 68 + CviJI RGCY 66 66 . . 92 95 + Csp6I GTAC 92 94 . . 92 95 + RsaI GTAC 93 93 . . 94 97 + SetI ASST 97 93 . . 118 122 + Tsp4CI* ACNGT 120 119 . . 126 131 - GsaI CCCAGC 130 126 . . 126 131 - BseYI CCCAGC 126 130 . . 132 137 + StuI AGGCCT 134 134 . . 133 136 + CviJI RGCY 134 134 . . 133 136 + HaeIII GGCC 134 134 . . 131 134 - MnlI CCTC 124 123 . . 135 140 + StyI CCWWGG 135 139 . . 135 140 + SecI* CCNNGG 135 139 . . 144 147 + SetI ASST 147 143 . . 146 151 + Hpy166II GTNNAC 148 148 . . 153 158 + ApoI RAATTY 153 157 . . 154 157 + TspEI AATT 153 157 . . 160 164 + HphI GGTGA 172 171 . . 161 165 + Tsp45I GTSAC 160 165 . . 161 165 + MaeIII GTNAC 160 165 . . 171 174 + SetI ASST 174 170 . . 180 186 + TspRI CASTGNN 186 177 . . 182 187 + BsgI GTGCAG 203 201 . . 183 186 + CviRI* TGCA 184 184 . . 206 211 + DsaI* CCRYGG 206 210 . . 206 211 + StyI CCWWGG 206 210 . . 206 211 + NcoI CCATGG 206 210 . . 206 211 + SecI* CCNNGG 206 210 . . 207 210 + FatI CATG 206 210 . . 207 210 + NlaIII CATG 210 206 . . 207 210 + CviAII CATG 207 209 . . 212 215 + Csp6I GTAC 212 214 . . 212 215 + RsaI GTAC 213 213 . . 225 228 + FnuDII* CGCG 226 226 . . 226 229 + Hin6I GCGC 226 228 . . 226 229 + GlaI GCGC 227 227 . . 226 229 + HhaI GCGC 228 226 . . 232 236 + MaeIII GTNAC 231 236 . . 241 247 + TspRI CASTGNN 247 238 . . 242 246 + BsrI ACTGG 247 245 . . 243 248 + Eco57MI CTGRAG 264 262 . . 243 248 + GsuI CTGGAG 264 262 . . 249 252 + SetI ASST 252 248 . . 249 252 + CviJI RGCY 250 250 . . 249 252 + AluI AGCT 250 250 . . 250 254 + BlsI GCNGC 252 251 . . 250 254 + BisI GCNGC 251 252 . . 250 254 + TseI GCWGC 250 253 . . 253 257 + BlsI GCNGC 255 254 . . 253 257 + BisI GCNGC 254 255 . . 253 257 + TseI GCWGC 253 256 . . 250 254 - BbvI GCAGC 237 241 . . 251 256 - EcoP15I CAGCAG 223 225 . . 253 257 - BbvI GCAGC 240 244 . . 264 267 + TaiI ACGT 267 263 . . 264 267 + SetI ASST 267 263 . . 264 267 + MaeII ACGT 264 266 . . 271 275 + BinI* GGATC 279 280 . . 269 272 - MnlI CCTC 262 261 . . 272 275 + DpnI GATC 273 273 . . 272 275 + BstKTI GATC 274 272 . . 272 275 + MboI GATC 271 275 . . 276 279 + SetI ASST 279 275 . . 276 282 + PpuMI RGGWCCY 277 280 . . 276 282 + DraII RGGNCCY 277 280 . . 277 281 + BmgT120I GGNCC 278 279 . . 277 281 + AvaII GGWCC 277 280 . . 277 281 + AsuI* GGNCC 277 280 . . 297 303 + DraII RGGNCCY 298 301 . . 297 302 + NlaIV GGNNCC 299 299 . . 298 303 + SduI GDGCHC 302 298 . . 298 303 + Bsp120I GGGCCC 298 302 . . 298 303 + NlaIV GGNNCC 300 300 . . 298 302 + BmgT120I GGNCC 299 300 . . 298 303 + HgiJII* GRGCYC 302 298 . . 298 303 + BseSI GKGCMC 302 298 . . 298 302 + AsuI* GGNCC 298 301 . . 298 303 + ApaI GGGCCC 302 298 . . 299 302 + CviJI RGCY 300 300 . . 299 302 + HaeIII GGCC 300 300 . . 299 303 + BmgT120I GGNCC 300 301 . . 299 303 + AsuI* GGNCC 299 302 . . 352 355 + MseI TTAA 352 354 . . 362 367 + TatI WGTACW 362 366 . . 363 366 + Csp6I GTAC 363 365 . . 363 366 + RsaI GTAC 364 364 . . 370 374 + HgaI GACGC 379 384 . . 380 384 + DdeI CTNAG 380 383 . . #--------------------------------------- # # # AluI: # [AGCT] # 140 250 # # ApaI: # [GGGCCC] # 88 302 # # ApoI: # [RAATTY] # 153 237 # # AsuI*: # [GGNCC] # 1 21 91 277 # # AvaII: # [GGWCC] # 113 277 # # BbvI: # [GCAGC] # 3 150 237 # # BinI*: # [GGATC] # 111 279 # # BisI: # [GCNGC] # 3 136 251 # # BlsI: # [GCNGC] # 3 135 252 # # BmgT120I: # [GGNCC] # 1 21 90 278 # # BseBI: # [CCWGG] # 32 358 # # BseSI: # [GKGCMC] # 88 302 # # BseYI: # [CCCAGC] # 126 264 # # BsgI: # [GTGCAG] # 187 203 # # BsiI*: # [CACGAG] # 56 334 # # Bsp120I: # [GGGCCC] # 92 298 # # BsrI: # [ACTGG] # 143 247 # # BssKI: # [CCNGG] # 30 360 # # BstKTI: # [GATC] # 44 116 230 # # Csp6I: # [GTAC] # 27 92 120 151 # # CspCI: # [CAANNNNNGTGG] # 35 42 313 # # CviAII: # [CATG] # 183 207 # # CviJI: # [RGCY] # 50 66 68 90 116 # # CviRI*: # [TGCA] # 184 206 # # DdeI: # [CTNAG] # 10 380 # # DpnI: # [GATC] # 43 117 230 # # DraII: # [RGGNCCY] # 21 92 277 # # DsaI*: # [CCRYGG] # 184 206 # # Eco57MI: # [CTGRAG] # 126 264 # # EcoP15I: # [CAGCAG] # 167 223 # # EcoRII: # [CCWGG] # 30 360 # # FatI: # [CATG] # 184 206 # # FnuDII*: # [CGCG] # 164 226 # # GlaI: # [GCGC] # 163 227 # # GsaI: # [CCCAGC] # 130 260 # # GsuI: # [CTGGAG] # 126 264 # # HaeIII: # [GGCC] # 90 134 166 # # HgaI: # [GACGC] # 11 379 # # HgiJII*: # [GRGCYC] # 88 302 # # HhaI: # [GCGC] # 162 228 # # Hin6I: # [GCGC] # 164 226 # # HphI: # [GGTGA] # 172 218 # # Hpy166II: # [GTNNAC] # 148 242 # # MaeII: # [ACGT] # 126 264 # # MaeIII: # [GTNAC] # 71 159 160 # # MboI: # [GATC] # 41 119 230 # # MnlI: # [CCTC] # 124 128 138 # # MseI: # [TTAA] # 38 352 # # NcoI: # [CCATGG] # 184 206 # # NlaIII: # [CATG] # 180 210 # # NlaIV: # [GGNNCC] # 1 90 299 # # PpuMI: # [RGGWCCY] # 113 277 # # RsaI: # [GTAC] # 26 93 120 151 # # ScrFI: # [CCNGG] # 32 358 # # SduI: # [GDGCHC] # 88 302 # # SecI*: # [CCNNGG] # 31 71 104 184 # # SetI: # [ASST] # 12 15 27 33 50 64 # 78 111 # # StuI: # [AGGCCT] # 134 256 # # StyI: # [CCWWGG] # 71 135 184 # # TaiI: # [ACGT] # 123 267 # # TatI: # [WGTACW] # 28 362 # # TseI: # [GCWGC] # 3 137 250 # # Tsp45I: # [GTSAC] # 160 230 # # Tsp4CI*: # [ACNGT] # 120 270 # # TspEI: # [AATT] # 153 237 # # TspRI: # [CASTGNN] # 61 143 186 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 390 # Reported_sequences: 1 # Reported_hitcount: 99 #---------------------------------------