Restriction Fragments for YER046W-A



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YER046W-A on chromosome V from coordinates 243700 to 244029.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.10263.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 2 5 + CviRI* TGCA 3 3 . . 17 22 + ClaI ATCGAT 18 20 . . 18 21 + TaqI TCGA 18 20 . . 20 23 + DpnI GATC 21 21 . . 20 23 + BstKTI GATC 22 20 . . 20 23 + MboI GATC 19 23 . . 23 26 + AciI CCGC 23 25 . . 20 24 - BinI* GGATC 14 15 . . 31 36 + Hpy166II GTNNAC 33 33 . . 31 36 + SduI GDGCHC 35 31 . . 31 36 + BseSI GKGCMC 35 31 . . 31 36 + ApaLI GTGCAC 31 35 . . 31 36 + HgiAI* GWGCWC 35 31 . . 32 35 + CviRI* TGCA 33 33 . . 36 47 + TstI CACNNNNNNTCC 27 22 59 54 37 41 + Tsp4CI* ACNGT 39 38 . . 48 51 + SetI ASST 51 47 . . 48 51 + CviJI RGCY 49 49 . . 48 51 + AluI AGCT 49 49 . . 50 53 + MaeI CTAG 50 52 . . 53 56 + CviJI RGCY 54 54 . . 55 58 + MaeI CTAG 55 57 . . 59 64 + SspI AATATT 61 61 . . 106 115 + MslI CAYNNNNRTG 110 110 . . 111 116 + Hpy178III* TCNNGA 112 114 . . 111 116 + BspHI TCATGA 111 115 . . 112 115 + FatI CATG 111 115 . . 112 115 + NlaIII CATG 115 111 . . 112 115 + CviAII CATG 112 114 . . 113 117 + TspDTI ATGAA 128 126 . . 117 120 + SetI ASST 120 116 . . 124 127 + DpnI GATC 125 125 . . 124 127 + BstKTI GATC 126 124 . . 124 127 + MboI GATC 123 127 . . 126 130 + Hpy188I TCNGA 128 127 . . 124 128 - BinI* GGATC 118 119 . . 130 133 + TspEI AATT 129 133 . . 138 141 + CviJI RGCY 139 139 . . 150 153 + Csp6I GTAC 150 152 . . 150 153 + RsaI GTAC 151 151 . . 162 166 - TspGWI ACGGA 152 150 . . 167 171 + BssKI CCNGG 166 171 . . 167 171 + BseBI CCWGG 168 169 . . 167 172 + SecI* CCNNGG 167 171 . . 167 171 + ScrFI CCNGG 168 169 . . 167 171 + EcoRII CCWGG 166 171 . . 171 176 - BciVI GTATCC 165 164 . . 178 181 - MnlI CCTC 171 170 . . 181 184 + CviJI RGCY 182 182 . . 198 202 + Tsp4CI* ACNGT 200 199 . . 205 208 + MseI TTAA 205 207 . . 212 226 + XcmI CCANNNNNNNNNTGG 219 218 . . 217 222 + StuI AGGCCT 219 219 . . 218 221 + CviJI RGCY 219 219 . . 218 221 + HaeIII GGCC 219 219 . . 240 243 + MnlI CCTC 250 249 . . 263 268 + TaqII CACCCA 279 277 . . 262 266 - HphI GGTGA 254 253 . . 268 273 + XhoII RGATCY 268 272 . . 268 273 + BglII AGATCT 268 272 . . 269 272 + DpnI GATC 270 270 . . 269 272 + BstKTI GATC 271 269 . . 269 272 + MboI GATC 268 272 . . 280 285 + SduI GDGCHC 284 280 . . 280 285 + Bsp120I GGGCCC 280 284 . . 280 285 + NlaIV GGNNCC 282 282 . . 280 284 + BmgT120I GGNCC 281 282 . . 280 285 + HgiJII* GRGCYC 284 280 . . 280 285 + BseSI GKGCMC 284 280 . . 280 284 + AsuI* GGNCC 280 283 . . 280 285 + ApaI GGGCCC 284 280 . . 281 284 + CviJI RGCY 282 282 . . 281 284 + HaeIII GGCC 282 282 . . 281 285 + BmgT120I GGNCC 282 283 . . 281 285 + AsuI* GGNCC 281 284 . . 284 289 + DsaI* CCRYGG 284 288 . . 284 289 + StyI CCWWGG 284 288 . . 284 289 + NcoI CCATGG 284 288 . . 284 289 + SecI* CCNNGG 284 288 . . 285 288 + FatI CATG 284 288 . . 285 288 + NlaIII CATG 288 284 . . 285 288 + CviAII CATG 285 287 . . 289 293 + BlsI GCNGC 291 290 . . 289 293 + BisI GCNGC 290 291 . . 289 293 + BbvI GCAGC 301 305 . . 289 293 + TseI GCWGC 289 292 . . 292 296 + SfaNI GCATC 301 305 . . 292 302 + MwoI GCNNNNNNNGC 298 295 . . 296 300 + Hin4II* CCTTC 306 305 . . 293 297 - FokI GGATG 279 283 . . 293 297 - BseGI GGATG 292 290 . . 307 317 + Hin4I GAYNNNNNVTC 298 293 330 325 308 311 + TaiI ACGT 311 307 . . 308 311 + SetI ASST 311 307 . . 308 311 + MaeII ACGT 308 310 . . 321 326 + Hpy178III* TCNNGA 322 324 . . #--------------------------------------- # # # AciI: # [CCGC] # 23 307 # # AluI: # [AGCT] # 49 281 # # ApaI: # [GGGCCC] # 46 284 # # ApaLI: # [GTGCAC] # 31 299 # # AsuI*: # [GGNCC] # 1 49 280 # # BbvI: # [GCAGC] # 29 301 # # BciVI: # [GTATCC] # 165 165 # # BglII: # [AGATCT] # 62 268 # # BinI*: # [GGATC] # 14 104 212 # # BisI: # [GCNGC] # 40 290 # # BlsI: # [GCNGC] # 39 291 # # BmgT120I: # [GGNCC] # 1 48 281 # # BseBI: # [CCWGG] # 162 168 # # BseGI: # [GGATG] # 38 292 # # BseSI: # [GKGCMC] # 35 46 249 # # Bsp120I: # [GGGCCC] # 50 280 # # BspHI: # [TCATGA] # 111 219 # # BssKI: # [CCNGG] # 164 166 # # BstKTI: # [GATC] # 22 59 104 145 # # ClaI: # [ATCGAT] # 18 312 # # Csp6I: # [GTAC] # 150 180 # # CviAII: # [CATG] # 45 112 173 # # CviJI: # [RGCY] # 5 37 43 48 49 63 # 85 # # CviRI*: # [TGCA] # 3 30 297 # # DpnI: # [GATC] # 21 60 104 145 # # DsaI*: # [CCRYGG] # 46 284 # # EcoRII: # [CCWGG] # 164 166 # # FatI: # [CATG] # 46 111 173 # # FokI: # [GGATG] # 51 279 # # HaeIII: # [GGCC] # 48 63 219 # # HgiAI*: # [GWGCWC] # 35 295 # # HgiJII*: # [GRGCYC] # 46 284 # # Hin4I: # [GAYNNNNNVTC] # 0 32 298 # # Hin4II*: # [CCTTC] # 24 306 # # HphI: # [GGTGA] # 76 254 # # Hpy166II: # [GTNNAC] # 33 297 # # Hpy178III*: # [TCNNGA] # 8 112 210 # # Hpy188I: # [TCNGA] # 128 202 # # MaeI: # [CTAG] # 5 50 275 # # MaeII: # [ACGT] # 22 308 # # MboI: # [GATC] # 19 62 104 145 # # MnlI: # [CCTC] # 79 80 171 # # MseI: # [TTAA] # 125 205 # # MslI: # [CAYNNNNRTG] # 110 220 # # MwoI: # [GCNNNNNNNGC] # 32 298 # # NcoI: # [CCATGG] # 46 284 # # NlaIII: # [CATG] # 42 115 173 # # NlaIV: # [GGNNCC] # 48 282 # # RsaI: # [GTAC] # 151 179 # # ScrFI: # [CCNGG] # 162 168 # # SduI: # [GDGCHC] # 35 46 249 # # SecI*: # [CCNNGG] # 46 117 167 # # SetI: # [ASST] # 19 51 69 191 # # SfaNI: # [GCATC] # 29 301 # # SspI: # [AATATT] # 61 269 # # StuI: # [AGGCCT] # 111 219 # # StyI: # [CCWWGG] # 46 284 # # TaiI: # [ACGT] # 19 311 # # TaqI: # [TCGA] # 18 312 # # TaqII: # [CACCCA] # 51 279 # # TseI: # [GCWGC] # 41 289 # # Tsp4CI*: # [ACNGT] # 39 130 161 # # TspDTI: # [ATGAA] # 128 202 # # TspEI: # [AATT] # 129 201 # # TspGWI: # [ACGGA] # 152 178 # # TstI: # [CACNNNNNNTCC] # 27 32 271 # # XcmI: # [CCANNNNNNNNNTGG] # 111 219 # # XhoII: # [RGATCY] # 62 268 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 330 # Reported_sequences: 1 # Reported_hitcount: 96 #---------------------------------------