Restriction Fragments for PHM8/YER037W



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

PHM8/YER037W on chromosome V from coordinates 225889 to 226854.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.22360.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 27 30 + TspEI AATT 26 30 . . 66 71 + Hpy178III* TCNNGA 67 69 . . 77 80 + CviRI* TGCA 78 78 . . 81 86 + HindIII AAGCTT 81 85 . . 82 85 + SetI ASST 85 81 . . 82 85 + CviJI RGCY 83 83 . . 82 85 + AluI AGCT 83 83 . . 94 97 + MaeI CTAG 94 96 . . 97 100 + CviJI RGCY 98 98 . . 109 112 + TspEI AATT 108 112 . . 113 116 + TaqI TCGA 113 115 . . 116 119 + SetI ASST 119 115 . . 115 118 - MnlI CCTC 108 107 . . 120 125 + XhoII RGATCY 120 124 . . 120 125 + NlaIV GGNNCC 122 122 . . 120 125 + BamHI GGATCC 120 124 . . 120 124 + BinI* GGATC 128 129 . . 121 124 + DpnI GATC 122 122 . . 121 124 + BstKTI GATC 123 121 . . 121 124 + MboI GATC 120 124 . . 124 127 + MnlI CCTC 134 133 . . 121 125 - BinI* GGATC 115 116 . . 131 134 + TspEI AATT 130 134 . . 135 140 + BetI* WCCGGW 135 139 . . 136 139 + HpaII CCGG 136 138 . . 138 143 + XhoII RGATCY 138 142 . . 138 143 + NlaIV GGNNCC 140 140 . . 138 143 + BamHI GGATCC 138 142 . . 138 142 + BinI* GGATC 146 147 . . 139 142 + DpnI GATC 140 140 . . 139 142 + BstKTI GATC 141 139 . . 139 148 + BsaBI GATNNNNATC 143 143 . . 139 142 + MboI GATC 138 142 . . 141 146 + Hpy178III* TCNNGA 142 144 . . 141 146 + BetI* WCCGGW 141 145 . . 141 146 + BspMII* TCCGGA 141 145 . . 142 145 + HpaII CCGG 142 144 . . 139 143 - BinI* GGATC 133 134 . . 144 149 + XhoII RGATCY 144 148 . . 144 149 + NlaIV GGNNCC 146 146 . . 144 149 + BamHI GGATCC 144 148 . . 144 148 + BinI* GGATC 152 153 . . 145 148 + DpnI GATC 146 146 . . 145 148 + BstKTI GATC 147 145 . . 145 148 + MboI GATC 144 148 . . 145 149 - BinI* GGATC 139 140 . . 153 156 + TaqI TCGA 153 155 . . 172 177 + EcoRV GATATC 174 174 . . 175 180 + ClaI ATCGAT 176 178 . . 176 179 + TaqI TCGA 176 178 . . 188 193 + TatI WGTACW 188 192 . . 188 193 + Bsp1407I TGTACA 188 192 . . 189 192 + Csp6I GTAC 189 191 . . 189 192 + RsaI GTAC 190 190 . . 206 209 + SetI ASST 209 205 . . 204 208 - Hin4II* CCTTC 198 197 . . 208 211 + Csp6I GTAC 208 210 . . 208 211 + RsaI GTAC 209 209 . . 211 216 + MfeI CAATTG 211 215 . . 212 215 + TspEI AATT 211 215 . . 219 222 + FatI CATG 218 222 . . 219 222 + NlaIII CATG 222 218 . . 219 222 + CviAII CATG 219 221 . . 221 224 + CviRI* TGCA 222 222 . . 237 242 + ApoI RAATTY 237 241 . . 238 241 + TspEI AATT 237 241 . . 244 249 + AhaIII* TTTAAA 246 246 . . 245 248 + MseI TTAA 245 247 . . 254 257 + TspEI AATT 253 257 . . 267 271 + Hpy99I CGWCG 271 266 . . 274 277 - MnlI CCTC 267 266 . . 290 293 + TaqI TCGA 290 292 . . 292 296 + TfiI GAWTC 292 295 . . 292 296 + HinfI GANTC 292 295 . . 303 308 + Hpy178III* TCNNGA 304 306 . . 319 323 - TspGWI ACGGA 309 307 . . 313 324 - TstI CACNNNNNNTCC 337 332 305 300 327 330 + SetI ASST 330 326 . . 331 334 + MseI TTAA 331 333 . . 348 351 + TspEI AATT 347 351 . . 355 360 + ZraI GACGTC 357 357 . . 355 360 + AcyI GRCGYC 356 358 . . 355 360 + AatII GACGTC 359 355 . . 356 359 + TaiI ACGT 359 355 . . 356 359 + SetI ASST 359 355 . . 356 359 + MaeII ACGT 356 358 . . 379 384 + ClaI ATCGAT 380 382 . . 376 380 - TspDTI ATGAA 366 364 . . 380 383 + TaqI TCGA 380 382 . . 385 389 + TfiI GAWTC 385 388 . . 385 389 + HinfI GANTC 385 388 . . 393 396 + SetI ASST 396 392 . . 398 401 + CviRI* TGCA 399 399 . . 408 413 + AhaIII* TTTAAA 410 410 . . 409 412 + MseI TTAA 409 411 . . 413 416 + CviJI RGCY 414 414 . . 427 430 + MseI TTAA 427 429 . . 434 437 + SetI ASST 437 433 . . 434 437 + CviJI RGCY 435 435 . . 434 437 + AluI AGCT 435 435 . . 435 439 + BlsI GCNGC 437 436 . . 435 439 + BisI GCNGC 436 437 . . 435 439 + TseI GCWGC 435 438 . . 435 439 - BbvI GCAGC 422 426 . . 440 445 + BclI TGATCA 440 444 . . 436 441 - EcoP15I CAGCAG 408 410 . . 441 444 + DpnI GATC 442 442 . . 441 444 + BstKTI GATC 443 441 . . 441 444 + MboI GATC 440 444 . . 445 448 + TspEI AATT 444 448 . . 447 452 + AhaIII* TTTAAA 449 449 . . 448 451 + MseI TTAA 448 450 . . 461 464 + SetI ASST 464 460 . . 461 464 + CviJI RGCY 462 462 . . 461 464 + AluI AGCT 462 462 . . 463 468 - NmeAIII GCCGAG 443 441 . . 469 474 + ApoI RAATTY 469 473 . . 470 473 + TspEI AATT 469 473 . . 485 488 + CviJI RGCY 486 486 . . 489 494 + Hpy166II GTNNAC 491 491 . . 501 504 + Csp6I GTAC 501 503 . . 501 504 + RsaI GTAC 502 502 . . 511 514 + FatI CATG 510 514 . . 511 514 + NlaIII CATG 514 510 . . 511 514 + CviAII CATG 511 513 . . 515 519 + BccI CCATC 523 524 . . 518 522 + Hpy188I TCNGA 520 519 . . 527 530 + MseI TTAA 527 529 . . 531 536 + SspI AATATT 533 533 . . 537 540 + SetI ASST 540 536 . . 547 550 + DpnI GATC 548 548 . . 547 550 + BstKTI GATC 549 547 . . 547 550 + MboI GATC 546 550 . . 557 561 + BceAI ACGGC 573 575 . . 565 568 + SetI ASST 568 564 . . 587 591 + BccI CCATC 595 596 . . 590 593 + TaqI TCGA 590 592 . . 592 595 - MnlI CCTC 585 584 . . 595 599 + MboII GAAGA 607 606 . . 598 603 + EcoRI GAATTC 598 602 . . 598 603 + ApoI RAATTY 598 602 . . 599 602 + TspEI AATT 598 602 . . 601 605 - TspDTI ATGAA 591 589 . . 607 610 + CviRI* TGCA 608 608 . . 608 613 + Cac8I GCNNGC 610 610 . . 611 614 + CviJI RGCY 612 612 . . 615 620 + XhoII RGATCY 615 619 . . 616 619 + DpnI GATC 617 617 . . 616 619 + BstKTI GATC 618 616 . . 616 619 + MboI GATC 615 619 . . 616 620 - BinI* GGATC 610 611 . . 622 627 + ApoI RAATTY 622 626 . . 623 626 + TspEI AATT 622 626 . . 628 633 + AsuII TTCGAA 629 631 . . 629 632 + TaqI TCGA 629 631 . . 626 630 - MboII GAAGA 618 617 . . 636 639 + SetI ASST 639 635 . . 636 639 + CviJI RGCY 637 637 . . 636 639 + AluI AGCT 637 637 . . 641 644 + TspEI AATT 640 644 . . 644 647 + CviRI* TGCA 645 645 . . 658 661 + TaqI TCGA 658 660 . . 661 664 + SetI ASST 664 660 . . 661 664 + CviJI RGCY 662 662 . . 661 664 + AluI AGCT 662 662 . . 666 669 + CviRI* TGCA 667 667 . . 674 678 + BssKI CCNGG 673 678 . . 674 678 + BseBI CCWGG 675 676 . . 674 678 + ScrFI CCNGG 675 676 . . 674 678 + EcoRII CCWGG 673 678 . . 698 703 + BsrDI GCAATG 705 703 . . 705 708 - AciI CCGC 705 707 . . 710 713 + Hin6I GCGC 710 712 . . 710 713 + GlaI GCGC 711 711 . . 710 720 + MwoI GCNNNNNNNGC 716 713 . . 710 713 + HhaI GCGC 712 710 . . 711 714 + FnuDII* CGCG 712 712 . . 720 729 + MslI CAYNNNNRTG 724 724 . . 720 723 + FatI CATG 719 723 . . 720 723 + NlaIII CATG 723 719 . . 720 723 + CviAII CATG 720 722 . . 725 729 + FokI GGATG 738 742 . . 725 729 + BseGI GGATG 731 729 . . 729 733 + BmgT120I GGNCC 730 731 . . 729 733 + AsuI* GGNCC 729 732 . . 730 733 + CviJI RGCY 731 731 . . 730 733 + HaeIII GGCC 731 731 . . 726 730 - BccI CCATC 720 721 . . 733 736 + FatI CATG 732 736 . . 733 736 + NlaIII CATG 736 732 . . 733 736 + CviAII CATG 733 735 . . 751 754 - MnlI CCTC 744 743 . . 766 770 + PleI GAGTC 774 775 . . 766 770 + MlyI GAGTC 775 775 . . 766 770 + HinfI GANTC 766 769 . . 769 773 + Hpy188I TCNGA 771 770 . . 775 780 + SspI AATATT 777 777 . . 781 785 + MaeIII GTNAC 780 785 . . 785 789 + DdeI CTNAG 785 788 . . 789 793 + BmgT120I GGNCC 790 791 . . 789 793 + AvaII GGWCC 789 792 . . 789 793 + AsuI* GGNCC 789 792 . . 809 812 + TspEI AATT 808 812 . . 824 834 + FalI AAGNNNNNCTT 815 810 847 842 826 831 + EcoRV GATATC 828 828 . . 832 837 + SmlI CTYRAG 832 836 . . 832 837 + Bce83I* CTTGAG 853 851 . . 824 834 - FalI AAGNNNNNCTT 847 842 815 810 837 841 + TfiI GAWTC 837 840 . . 837 841 + HinfI GANTC 837 840 . . 854 857 + TaiI ACGT 857 853 . . 854 857 + SetI ASST 857 853 . . 854 857 + MaeII ACGT 854 856 . . 862 867 + Hpy166II GTNNAC 864 864 . . 866 870 + Tsp4CI* ACNGT 868 867 . . 871 875 + BccI CCATC 879 880 . . 875 878 + MnlI CCTC 885 884 . . 872 876 - FokI GGATG 858 862 . . 872 876 - BseGI GGATG 871 869 . . 884 898 + XcmI CCANNNNNNNNNTGG 891 890 . . 898 902 + MboII GAAGA 910 909 . . 898 903 - Ksp632I* CTCTTC 893 896 . . 907 911 + MboII GAAGA 919 918 . . 903 908 - MmeI TCCRAC 884 882 . . 907 912 - Ksp632I* CTCTTC 902 905 . . 910 913 - MnlI CCTC 903 902 . . 913 917 - Hin4II* CCTTC 907 906 . . 925 930 + Hpy166II GTNNAC 927 927 . . 925 930 + HindII GTYRAC 927 927 . . 929 933 + BsrI ACTGG 934 932 . . 945 948 + DpnI GATC 946 946 . . 945 948 + BstKTI GATC 947 945 . . 945 948 + MboI GATC 944 948 . . 961 966 + Hpy178III* TCNNGA 962 964 . . 961 966 + BspHI TCATGA 961 965 . . 962 965 + FatI CATG 961 965 . . 962 965 + NlaIII CATG 965 961 . . 962 965 + CviAII CATG 962 964 . . #--------------------------------------- # # # AatII: # [GACGTC] # 359 607 # # AciI: # [CCGC] # 261 705 # # AcyI: # [GRCGYC] # 356 610 # # AhaIII*: # [TTTAAA] # 39 164 246 517 # # AluI: # [AGCT] # 25 27 83 175 304 352 # # ApoI: # [RAATTY] # 24 129 232 237 344 # # AsuI*: # [GGNCC] # 60 177 729 # # AsuII: # [TTCGAA] # 337 629 # # AvaII: # [GGWCC] # 177 789 # # BamHI: # [GGATCC] # 6 18 120 822 # # BbvI: # [GCAGC] # 422 544 # # BccI: # [CCATC] # 72 87 125 159 523 # # Bce83I*: # [CTTGAG] # 113 853 # # BceAI: # [ACGGC] # 393 573 # # BclI: # [TGATCA] # 440 526 # # BetI*: # [WCCGGW] # 6 135 825 # # BinI*: # [GGATC] # 5 6 6 7 13 115 # 356 458 # # BisI: # [GCNGC] # 436 530 # # BlsI: # [GCNGC] # 437 529 # # BmgT120I: # [GGNCC] # 60 176 730 # # BsaBI: # [GATNNNNATC] # 143 823 # # BseBI: # [CCWGG] # 291 675 # # BseGI: # [GGATG] # 95 140 731 # # Bsp1407I: # [TGTACA] # 188 778 # # BspHI: # [TCATGA] # 5 961 # # BspMII*: # [TCCGGA] # 141 825 # # BsrDI: # [GCAATG] # 261 705 # # BsrI: # [ACTGG] # 32 934 # # BssKI: # [CCNGG] # 293 673 # # BstKTI: # [GATC] # 6 18 19 69 106 123 # 296 329 # # Cac8I: # [GCNNGC] # 356 610 # # ClaI: # [ATCGAT] # 176 204 586 # # Csp6I: # [GTAC] # 19 189 293 465 # # CviAII: # [CATG] # 4 13 209 219 229 292 # # CviJI: # [RGCY] # 15 21 24 25 25 27 # 69 83 126 235 316 # # CviRI*: # [TGCA] # 22 37 78 144 177 209 # 299 # # DdeI: # [CTNAG] # 181 785 # # DpnI: # [GATC] # 6 18 20 69 106 122 # 296 329 # # EcoP15I: # [CAGCAG] # 408 558 # # EcoRI: # [GAATTC] # 368 598 # # EcoRII: # [CCWGG] # 293 673 # # EcoRV: # [GATATC] # 138 174 654 # # FalI: # [AAGNNNNNCTT] # 0 0 32 119 815 # # FatI: # [CATG] # 5 13 209 218 229 292 # # FnuDII*: # [CGCG] # 254 712 # # FokI: # [GGATG] # 108 120 738 # # GlaI: # [GCGC] # 255 711 # # HaeIII: # [GGCC] # 235 731 # # HhaI: # [GCGC] # 254 712 # # Hin4II*: # [CCTTC] # 59 198 709 # # Hin6I: # [GCGC] # 256 710 # # HindII: # [GTYRAC] # 39 927 # # HindIII: # [AAGCTT] # 81 885 # # HinfI: # [GANTC] # 71 93 129 292 381 # # HpaII: # [CCGG] # 6 136 824 # # Hpy166II: # [GTNNAC] # 39 63 373 491 # # Hpy178III*: # [TCNNGA] # 4 67 75 162 658 # # Hpy188I: # [TCNGA] # 195 251 520 # # Hpy99I: # [CGWCG] # 271 695 # # Ksp632I*: # [CTCTTC] # 9 64 893 # # MaeI: # [CTAG] # 94 872 # # MaeII: # [ACGT] # 112 356 498 # # MaeIII: # [GTNAC] # 186 780 # # MboI: # [GATC] # 6 18 22 69 106 120 # 296 329 # # MboII: # [GAAGA] # 9 11 47 292 607 # # MfeI: # [CAATTG] # 211 755 # # MlyI: # [GAGTC] # 191 775 # # MmeI: # [TCCRAC] # 82 884 # # MnlI: # [CCTC] # 18 26 63 108 133 141 # 159 318 # # MseI: # [TTAA] # 18 21 78 79 86 245 # 439 # # MslI: # [CAYNNNNRTG] # 242 724 # # MwoI: # [GCNNNNNNNGC] # 250 716 # # NlaIII: # [CATG] # 1 13 209 222 229 292 # # NlaIV: # [GGNNCC] # 6 18 122 820 # # NmeAIII: # [GCCGAG] # 443 523 # # PleI: # [GAGTC] # 192 774 # # RsaI: # [GTAC] # 19 190 293 464 # # ScrFI: # [CCNGG] # 291 675 # # SetI: # [ASST] # 25 27 28 29 34 37 # 41 71 76 85 90 109 # 121 193 # # SmlI: # [CTYRAG] # 134 832 # # SspI: # [AATATT] # 189 244 533 # # TaiI: # [ACGT] # 109 359 498 # # TaqI: # [TCGA] # 23 29 39 40 90 113 # 114 210 308 # # TatI: # [WGTACW] # 188 778 # # TfiI: # [GAWTC] # 93 129 292 452 # # TseI: # [GCWGC] # 435 531 # # Tsp4CI*: # [ACNGT] # 98 868 # # TspDTI: # [ATGAA] # 225 366 375 # # TspEI: # [AATT] # 16 18 22 24 25 26 # 26 81 82 94 97 129 # 158 168 # # TspGWI: # [ACGGA] # 309 657 # # TstI: # [CACNNNNNNTCC] # 32 305 629 # # XcmI: # [CCANNNNNNNNNTGG] # 75 891 # # XhoII: # [RGATCY] # 6 18 120 351 471 # # ZraI: # [GACGTC] # 357 609 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 966 # Reported_sequences: 1 # Reported_hitcount: 238 #---------------------------------------