Restriction Fragments for GPA2/YER020W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

GPA2/YER020W on chromosome V from coordinates 195168 to 196517.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.27036.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 4 9 + Eco31I GGTCTC 10 14 . . 5 9 + BsmAI GTCTC 10 14 . . 10 15 + MstI* TGCGCA 12 12 . . 11 14 + Hin6I GCGC 11 13 . . 11 14 + GlaI GCGC 12 12 . . 11 14 + HhaI GCGC 13 11 . . 13 17 + SfaNI GCATC 22 26 . . 19 23 + Hpy188I TCNGA 21 20 . . 16 20 - MboII GAAGA 8 7 . . 29 33 + BceAI ACGGC 45 47 . . 32 36 + BlsI GCNGC 34 33 . . 32 36 + BisI GCNGC 33 34 . . 32 36 + BbvI GCAGC 44 48 . . 32 36 + TseI GCWGC 32 35 . . 39 44 + Hpy178III* TCNNGA 40 42 . . 53 56 + AciI CCGC 53 55 . . 55 60 + Cac8I GCNNGC 57 57 . . 58 63 + HaeII RGCGCY 62 58 . . 58 63 + Eco47III AGCGCT 60 60 . . 59 62 + Hin6I GCGC 59 61 . . 59 62 + GlaI GCGC 60 60 . . 59 62 + HhaI GCGC 61 59 . . 68 72 + Tsp45I GTSAC 67 72 . . 68 72 + MaeIII GTNAC 67 72 . . 73 78 + AclI AACGTT 74 76 . . 74 77 + TaiI ACGT 77 73 . . 74 77 + SetI ASST 77 73 . . 74 77 + MaeII ACGT 74 76 . . 89 92 + SetI ASST 92 88 . . 90 95 + KpnI GGTACC 94 90 . . 90 95 + NlaIV GGNNCC 92 92 . . 90 95 + HgiCI* GGYRCC 90 94 . . 90 95 + Acc65I GGTACC 90 94 . . 87 91 - Hin4II* CCTTC 81 80 . . 91 94 + Csp6I GTAC 91 93 . . 91 94 + RsaI GTAC 92 92 . . 105 110 + NlaIV GGNNCC 107 107 . . 107 110 + CviJI RGCY 108 108 . . 114 118 + MboII GAAGA 126 125 . . 114 119 + BbvII* GAAGAC 121 125 . . 118 122 + Tsp4CI* ACNGT 120 119 . . 122 133 + BdaI TGANNNNNNTCA 111 109 145 143 127 131 + Tsp4CI* ACNGT 129 128 . . 130 135 + Hpy166II GTNNAC 132 132 . . 130 135 + HindII GTYRAC 132 132 . . 122 133 - BdaI TGANNNNNNTCA 145 143 111 109 165 168 - MnlI CCTC 158 157 . . 168 178 + MwoI GCNNNNNNNGC 174 171 . . 168 172 + BlsI GCNGC 170 169 . . 168 172 + BisI GCNGC 169 170 . . 168 172 + BbvI GCAGC 180 184 . . 168 172 + TseI GCWGC 168 171 . . 169 174 + EcoP15I CAGCAG 199 201 . . 171 175 + BlsI GCNGC 173 172 . . 171 175 + BisI GCNGC 172 173 . . 171 175 + BbvI GCAGC 183 187 . . 171 175 + TseI GCWGC 171 174 . . 172 177 + EcoP15I CAGCAG 202 204 . . 174 178 + BlsI GCNGC 176 175 . . 174 178 + BisI GCNGC 175 176 . . 174 178 + BbvI GCAGC 186 190 . . 174 178 + TseI GCWGC 174 177 . . 176 179 + CviJI RGCY 177 177 . . 177 187 + MwoI GCNNNNNNNGC 183 180 . . 179 184 + Esp3I CGTCTC 185 189 . . 180 184 + BsmAI GTCTC 185 189 . . 177 181 - BceAI ACGGC 162 164 . . 184 187 + AciI CCGC 184 186 . . 194 197 + MseI TTAA 194 196 . . 198 202 + BmgT120I GGNCC 199 200 . . 198 202 + AvaII GGWCC 198 201 . . 198 202 + AsuI* GGNCC 198 201 . . 201 204 + AciI CCGC 201 203 . . 223 228 + VspI ATTAAT 224 226 . . 224 227 + MseI TTAA 224 226 . . 231 234 + FnuDII* CGCG 232 232 . . 234 239 + EcoRV GATATC 236 236 . . 242 247 + DsaI* CCRYGG 242 246 . . 242 247 + SecI* CCNNGG 242 246 . . 244 248 + BceAI ACGGC 260 262 . . 246 249 + CviJI RGCY 247 247 . . 250 254 + BceAI ACGGC 266 268 . . 265 269 + BinI* GGATC 273 274 . . 266 269 + DpnI GATC 267 267 . . 266 269 + BstKTI GATC 268 266 . . 266 269 + MboI GATC 265 269 . . 263 266 - AciI CCGC 263 265 . . 276 279 + DpnI GATC 277 277 . . 276 279 + BstKTI GATC 278 276 . . 276 285 + BsaBI GATNNNNATC 280 280 . . 276 279 + MboI GATC 275 279 . . 284 287 + TaqI TCGA 284 286 . . 286 290 + HinfI GANTC 286 289 . . 286 290 - PleI GAGTC 280 281 . . 286 290 - MlyI GAGTC 280 280 . . 295 299 + DdeI CTNAG 295 298 . . 295 299 - BspCNI CTCAG 287 285 . . 295 299 - BseMII CTCAG 286 284 . . 299 303 - BsmAI GTCTC 293 297 . . 304 307 + SetI ASST 307 303 . . 307 310 + TaqI TCGA 307 309 . . 311 315 + MaeIII GTNAC 310 315 . . 313 318 + AclI AACGTT 314 316 . . 314 317 + TaiI ACGT 317 313 . . 314 317 + SetI ASST 317 313 . . 314 317 + MaeII ACGT 314 316 . . 318 321 + CviRI* TGCA 319 319 . . 319 323 + BlsI GCNGC 321 320 . . 319 323 + BisI GCNGC 320 321 . . 319 323 + BbvI GCAGC 331 335 . . 319 323 + TseI GCWGC 319 322 . . 328 332 + BccI CCATC 336 337 . . 337 341 + Hpy188I TCNGA 339 338 . . 340 344 + HgaI GACGC 349 354 . . 336 341 - MmeI TCCRAC 317 315 . . 353 357 + BlsI GCNGC 355 354 . . 353 357 + BisI GCNGC 354 355 . . 353 357 + BbvI GCAGC 365 369 . . 353 357 + TseI GCWGC 353 356 . . 382 387 - EcoP15I CAGCAG 354 356 . . 388 393 + NlaIV GGNNCC 390 390 . . 388 393 + HgiCI* GGYRCC 388 392 . . 384 389 - GsaI CCCAGC 388 384 . . 384 389 - BseYI CCCAGC 384 388 . . 386 391 - TaqII CACCCA 376 374 . . 391 396 + Cfr10I RCCGGY 391 395 . . 392 395 + HpaII CCGG 392 394 . . 394 398 + HphI GGTGA 406 405 . . 408 413 + Hpy166II GTNNAC 410 410 . . 410 415 + DsaI* CCRYGG 410 414 . . 410 415 + SecI* CCNNGG 410 414 . . 412 416 + Tsp4CI* ACNGT 414 413 . . 419 422 + CviRI* TGCA 420 420 . . 420 424 + BlsI GCNGC 422 421 . . 420 424 + BisI GCNGC 421 422 . . 420 424 + BbvI GCAGC 432 436 . . 420 424 + TseI GCWGC 420 423 . . 421 426 + EcoP15I CAGCAG 451 453 . . 429 433 + MboII GAAGA 441 440 . . 455 460 + Cac8I GCNNGC 457 457 . . 465 468 + TspEI AATT 464 468 . . 467 470 + MseI TTAA 467 469 . . 473 478 + TatI WGTACW 473 477 . . 474 477 + Csp6I GTAC 474 476 . . 474 477 + RsaI GTAC 475 475 . . 477 481 - FokI GGATG 463 467 . . 477 481 - BseGI GGATG 476 474 . . 486 489 + DpnI GATC 487 487 . . 486 489 + BstKTI GATC 488 486 . . 486 489 + MboI GATC 485 489 . . 492 496 + Hpy188I TCNGA 494 493 . . 495 499 + TfiI GAWTC 495 498 . . 495 499 + HinfI GANTC 495 498 . . 507 510 + TspEI AATT 506 510 . . 515 520 + NlaIV GGNNCC 517 517 . . 518 521 + SetI ASST 521 517 . . 519 522 + MnlI CCTC 529 528 . . 519 529 + EcoNI CCTNNNNNAGG 523 524 . . 519 529 + BsiYI* CCNNNNNNNGG 525 522 . . 525 529 + BssKI CCNGG 524 529 . . 525 529 + BseBI CCWGG 526 527 . . 525 529 + ScrFI CCNGG 526 527 . . 525 529 + EcoRII CCWGG 524 529 . . 522 526 - FokI GGATG 508 512 . . 522 526 - BseGI GGATG 521 519 . . 538 541 + SetI ASST 541 537 . . 542 545 + MseI TTAA 542 544 . . 545 548 + TaiI ACGT 548 544 . . 545 548 + SetI ASST 548 544 . . 545 548 + MaeII ACGT 545 547 . . 547 552 + Hpy166II GTNNAC 549 549 . . 547 552 + HindII GTYRAC 549 549 . . 555 560 + NlaIV GGNNCC 557 557 . . 558 563 + BetI* WCCGGW 558 562 . . 559 562 + HpaII CCGG 559 561 . . 555 566 - TstI CACNNNNNNTCC 579 574 547 542 566 571 + Hpy166II GTNNAC 568 568 . . 573 577 + HgaI GACGC 582 587 . . 584 587 + SetI ASST 587 583 . . 589 592 + TaqI TCGA 589 591 . . 589 594 + Hpy178III* TCNNGA 590 592 . . 597 600 + FatI CATG 596 600 . . 597 600 + NlaIII CATG 600 596 . . 597 600 + CviAII CATG 597 599 . . 605 609 + TspDTI ATGAA 620 618 . . 627 631 + BmgT120I GGNCC 628 629 . . 627 631 + AsuI* GGNCC 627 630 . . 628 631 + CviJI RGCY 629 629 . . 628 631 + HaeIII GGCC 629 629 . . 630 640 + BsiYI* CCNNNNNNNGG 636 633 . . 632 635 + TspEI AATT 631 635 . . 635 641 + PfoI TCCNGGA 635 640 . . 636 640 + BssKI CCNGG 635 640 . . 636 640 + ScrFI CCNGG 637 638 . . 636 640 + CauII* CCSGG 637 638 . . 637 640 + HpaII CCGG 637 639 . . 640 644 + MboII GAAGA 652 651 . . 643 648 + EcoRV GATATC 645 645 . . 648 651 + FnuDII* CGCG 649 649 . . 649 654 + Cac8I GCNNGC 651 651 . . 649 652 - AciI CCGC 649 651 . . 649 653 - FauI CCCGC 642 644 . . 664 667 + TaiI ACGT 667 663 . . 664 667 + SetI ASST 667 663 . . 664 667 + MaeII ACGT 664 666 . . 671 675 + BmgT120I GGNCC 672 673 . . 671 675 + AsuI* GGNCC 671 674 . . 672 675 + CviJI RGCY 673 673 . . 672 675 + HaeIII GGCC 673 673 . . 680 683 + MnlI CCTC 690 689 . . 703 707 + BmgT120I GGNCC 704 705 . . 703 707 + AsuI* GGNCC 703 706 . . 704 707 + CviJI RGCY 705 705 . . 704 707 + HaeIII GGCC 705 705 . . 712 716 + SfaNI GCATC 721 725 . . 715 718 + TaqI TCGA 715 717 . . 733 737 + HinfI GANTC 733 736 . . 736 739 + TaqI TCGA 736 738 . . 733 737 - PleI GAGTC 727 728 . . 733 737 - MlyI GAGTC 727 727 . . 738 742 + HinfI GANTC 738 741 . . 738 742 - PleI GAGTC 732 733 . . 738 742 - MlyI GAGTC 732 732 . . 750 753 + FatI CATG 749 753 . . 750 753 + NlaIII CATG 753 749 . . 750 753 + CviAII CATG 750 752 . . 748 752 - TspDTI ATGAA 738 736 . . 756 761 + ApoI RAATTY 756 760 . . 757 760 + TspEI AATT 756 760 . . 764 768 + BssKI CCNGG 763 768 . . 764 768 + BseBI CCWGG 765 766 . . 764 768 + ScrFI CCNGG 765 766 . . 764 768 + EcoRII CCWGG 763 768 . . 761 765 - HphI GGTGA 753 752 . . 767 771 + BinI* GGATC 775 776 . . 768 771 + DpnI GATC 769 769 . . 768 771 + BstKTI GATC 770 768 . . 768 771 + MboI GATC 767 771 . . 781 784 + TspEI AATT 780 784 . . 792 797 + TaqII CACCCA 808 806 . . 794 799 + GsaI CCCAGC 798 794 . . 794 799 + BseYI CCCAGC 794 798 . . 796 801 + EcoP15I CAGCAG 826 828 . . 808 811 + MseI TTAA 808 810 . . 812 815 + DpnI GATC 813 813 . . 812 815 + BstKTI GATC 814 812 . . 812 815 + MboI GATC 811 815 . . 814 817 + TaqI TCGA 814 816 . . 814 819 + Hpy178III* TCNNGA 815 817 . . 816 820 - BsmAI GTCTC 810 814 . . 825 830 + ZraI GACGTC 827 827 . . 825 830 + AcyI GRCGYC 826 828 . . 825 830 + AatII GACGTC 829 825 . . 826 829 + TaiI ACGT 829 825 . . 826 829 + SetI ASST 829 825 . . 826 829 + MaeII ACGT 826 828 . . 841 849 + Tth111I GACNNNGTC 844 845 . . 844 848 + Tsp4CI* ACNGT 846 845 . . 849 858 + MslI CAYNNNNRTG 853 853 . . 853 863 + Hin4I GAYNNNNNVTC 844 839 876 871 862 866 + Hpy188I TCNGA 864 863 . . 853 863 - Hin4I GAYNNNNNVTC 876 871 844 839 865 870 + EcoRV GATATC 867 867 . . 861 866 - MmeI TCCRAC 842 840 . . 869 874 + Hpy178III* TCNNGA 870 872 . . 874 879 + EcoT22I ATGCAT 878 874 . . 875 878 + CviRI* TGCA 876 876 . . 873 877 - SfaNI GCATC 863 867 . . 885 889 + Hpy99I CGWCG 889 884 . . 887 890 + TaiI ACGT 890 886 . . 887 890 + SetI ASST 890 886 . . 887 890 + MaeII ACGT 887 889 . . 886 891 - BtrI CACGTC 888 888 . . 893 898 + Hpy166II GTNNAC 895 895 . . 890 895 - TaqII CACCCA 880 878 . . 904 908 + Hpy188I TCNGA 906 905 . . 925 931 + TspRI CASTGNN 931 922 . . 920 925 - BciVI GTATCC 914 913 . . 925 930 - BtsI GCAGTG 924 922 . . 932 935 + TaqI TCGA 932 934 . . 934 942 + Tth111I GACNNNGTC 937 938 . . 940 944 + Tsp45I GTSAC 939 944 . . 940 944 + MaeIII GTNAC 939 944 . . 970 974 + Hpy188I TCNGA 972 971 . . 975 978 + Csp6I GTAC 975 977 . . 975 978 + RsaI GTAC 976 976 . . 982 990 + AlwNI CAGNNNCTG 987 984 . . 984 988 + HgaI GACGC 993 998 . . 990 994 - BccI CCATC 984 985 . . 994 997 - MnlI CCTC 987 986 . . 1012 1015 + SetI ASST 1015 1011 . . 1017 1022 + Hpy178III* TCNNGA 1018 1020 . . 1021 1025 + TfiI GAWTC 1021 1024 . . 1021 1025 + HinfI GANTC 1021 1024 . . 1037 1040 + TaqI TCGA 1037 1039 . . 1042 1047 + SspI AATATT 1044 1044 . . 1048 1053 + Hpy166II GTNNAC 1050 1050 . . 1048 1053 + HindII GTYRAC 1050 1050 . . 1052 1056 + Tsp4CI* ACNGT 1054 1053 . . 1058 1062 - BccI CCATC 1052 1053 . . 1065 1068 + FnuDII* CGCG 1066 1066 . . 1066 1069 + Hin6I GCGC 1066 1068 . . 1066 1069 + GlaI GCGC 1067 1067 . . 1066 1071 + Cac8I GCNNGC 1068 1068 . . 1066 1071 + BsePI GCGCGC 1066 1070 . . 1066 1069 + HhaI GCGC 1068 1066 . . 1067 1070 + FnuDII* CGCG 1068 1068 . . 1068 1071 + Hin6I GCGC 1068 1070 . . 1068 1071 + GlaI GCGC 1069 1069 . . 1068 1071 + HhaI GCGC 1070 1068 . . 1071 1076 + BtrI CACGTC 1073 1073 . . 1072 1075 + TaiI ACGT 1075 1071 . . 1072 1075 + SetI ASST 1075 1071 . . 1072 1075 + MaeII ACGT 1072 1074 . . 1081 1084 + Csp6I GTAC 1081 1083 . . 1070 1081 - BcgI CGANNNNNNTGC 1093 1091 1059 1057 1081 1084 + RsaI GTAC 1082 1082 . . 1089 1093 + Hpy188I TCNGA 1091 1090 . . 1100 1103 + TaqI TCGA 1100 1102 . . 1101 1112 + BcgI CGANNNNNNTGC 1090 1088 1124 1122 1103 1106 + SetI ASST 1106 1102 . . 1120 1124 + DdeI CTNAG 1120 1123 . . 1141 1144 + TspEI AATT 1140 1144 . . 1158 1165 + SgrAI CRCCGGYG 1159 1163 . . 1159 1164 + Cfr10I RCCGGY 1159 1163 . . 1160 1163 + HpaII CCGG 1160 1162 . . 1163 1166 - AciI CCGC 1163 1165 . . 1163 1167 - FauI CCCGC 1156 1158 . . 1168 1172 + Hpy188I TCNGA 1170 1169 . . 1182 1185 + CviJI RGCY 1183 1183 . . 1183 1187 + BlsI GCNGC 1185 1184 . . 1183 1187 + BisI GCNGC 1184 1185 . . 1183 1187 + TseI GCWGC 1183 1186 . . 1187 1191 + DdeI CTNAG 1187 1190 . . 1183 1187 - BbvI GCAGC 1170 1174 . . 1190 1195 + TatI WGTACW 1190 1194 . . 1191 1194 + Csp6I GTAC 1191 1193 . . 1191 1194 + RsaI GTAC 1192 1192 . . 1199 1204 + Hpy178III* TCNNGA 1200 1202 . . 1200 1205 + Eco57MI CTGRAG 1221 1219 . . 1200 1205 + GsuI CTGGAG 1221 1219 . . 1204 1207 + SetI ASST 1207 1203 . . 1203 1206 - MnlI CCTC 1196 1195 . . 1216 1219 + MseI TTAA 1216 1218 . . 1227 1231 + TfiI GAWTC 1227 1230 . . 1227 1231 + HinfI GANTC 1227 1230 . . 1231 1235 + DdeI CTNAG 1231 1234 . . 1243 1246 + MnlI CCTC 1253 1252 . . 1246 1251 + BsaAI YACGTR 1248 1248 . . 1246 1251 + PmaCI CACGTG 1248 1248 . . 1247 1250 + TaiI ACGT 1250 1246 . . 1247 1250 + SetI ASST 1250 1246 . . 1247 1250 + MaeII ACGT 1247 1249 . . 1249 1253 + Tsp45I GTSAC 1248 1253 . . 1249 1253 + MaeIII GTNAC 1248 1253 . . 1257 1260 + CviJI RGCY 1258 1258 . . 1257 1260 + HaeIII GGCC 1258 1258 . . 1265 1270 + AflIII ACRYGT 1265 1269 . . 1266 1271 + BtrI CACGTC 1268 1268 . . 1267 1270 + TaiI ACGT 1270 1266 . . 1267 1270 + SetI ASST 1270 1266 . . 1267 1270 + MaeII ACGT 1267 1269 . . 1268 1272 + Hpy99I CGWCG 1272 1267 . . 1270 1273 + TaqI TCGA 1270 1272 . . 1291 1295 + TauI GCSGC 1294 1291 . . 1291 1295 + BlsI GCNGC 1293 1292 . . 1291 1295 + BisI GCNGC 1292 1293 . . 1292 1295 + AciI CCGC 1292 1294 . . 1295 1299 + BccI CCATC 1303 1304 . . 1308 1311 + TspEI AATT 1307 1311 . . 1330 1334 + HinfI GANTC 1330 1333 . . 1333 1338 + Hpy178III* TCNNGA 1334 1336 . . 1330 1334 - PleI GAGTC 1324 1325 . . 1330 1334 - MlyI GAGTC 1324 1324 . . 1341 1345 + MaeIII GTNAC 1340 1345 . . #--------------------------------------- # # # AatII: # [GACGTC] # 521 829 # # Acc65I: # [GGTACC] # 90 1260 # # AciI: # [CCGC] # 17 53 58 62 129 131 # 386 514 # # AclI: # [AACGTT] # 74 240 1036 # # AcyI: # [GRCGYC] # 524 826 # # AflIII: # [ACRYGT] # 85 1265 # # AlwNI: # [CAGNNNCTG] # 363 987 # # ApoI: # [RAATTY] # 594 756 # # AsuI*: # [GGNCC] # 32 44 198 429 647 # # AvaII: # [GGWCC] # 198 1152 # # BbvI: # [GCAGC] # 3 3 34 44 67 136 # 145 180 738 # # BbvII*: # [GAAGAC] # 121 1229 # # BccI: # [CCATC] # 47 68 251 336 648 # # BceAI: # [ACGGC] # 6 45 98 117 1084 # # BcgI: # [CGANNNNNNTGC] # 3 31 31 226 1059 # # BciVI: # [GTATCC] # 436 914 # # BdaI: # [TGANNNNNNTCA] # 0 0 34 111 1205 # # BetI*: # [WCCGGW] # 558 792 # # BinI*: # [GGATC] # 273 502 575 # # BisI: # [GCNGC] # 3 3 33 34 58 67 # 108 136 145 763 # # BlsI: # [GCNGC] # 3 3 34 34 57 67 # 108 136 145 763 # # BmgT120I: # [GGNCC] # 32 44 199 429 646 # # BsaAI: # [YACGTR] # 102 1248 # # BsaBI: # [GATNNNNATC] # 280 1070 # # BseBI: # [CCWGG] # 239 526 585 # # BseGI: # [GGATG] # 45 476 829 # # BseMII: # [CTCAG] # 286 1064 # # BsePI: # [GCGCGC] # 284 1066 # # BseYI: # [CCCAGC] # 384 410 556 # # BsiYI*: # [CCNNNNNNNGG] # 111 525 714 # # BsmAI: # [GTCTC] # 10 108 175 517 540 # # BspCNI: # [CTCAG] # 287 1063 # # BssKI: # [CCNGG] # 111 128 524 587 # # BstKTI: # [GATC] # 10 44 210 268 282 536 # # BtrI: # [CACGTC] # 82 185 195 888 # # BtsI: # [GCAGTG] # 426 924 # # Cac8I: # [GCNNGC] # 57 194 282 400 417 # # CauII*: # [CCSGG] # 637 713 # # Cfr10I: # [RCCGGY] # 191 391 768 # # Csp6I: # [GTAC] # 91 106 110 159 383 501 # # CviAII: # [CATG] # 153 597 600 # # CviJI: # [RGCY] # 32 44 69 70 75 92 # 108 382 478 # # CviRI*: # [TGCA] # 101 319 456 474 # # DdeI: # [CTNAG] # 44 67 119 295 825 # # DpnI: # [GATC] # 10 44 210 267 282 537 # # DsaI*: # [CCRYGG] # 168 242 940 # # Eco31I: # [GGTCTC] # 10 1340 # # Eco47III: # [AGCGCT] # 60 1290 # # Eco57MI: # [CTGRAG] # 129 1221 # # EcoNI: # [CCTNNNNNAGG] # 523 827 # # EcoP15I: # [CAGCAG] # 3 97 152 199 375 524 # # EcoRII: # [CCWGG] # 239 524 587 # # EcoRV: # [GATATC] # 222 236 409 483 # # EcoT22I: # [ATGCAT] # 472 878 # # Esp3I: # [CGTCTC] # 185 1165 # # FatI: # [CATG] # 153 596 601 # # FauI: # [CCCGC] # 194 514 642 # # FnuDII*: # [CGCG] # 2 232 282 417 417 # # FokI: # [GGATG] # 45 463 842 # # GlaI: # [GCGC] # 2 12 48 281 1007 # # GsaI: # [CCCAGC] # 388 410 552 # # GsuI: # [CTGGAG] # 129 1221 # # HaeII: # [RGCGCY] # 62 1288 # # HaeIII: # [GGCC] # 32 44 92 553 629 # # HgaI: # [GACGC] # 233 349 357 411 # # HgiCI*: # [GGYRCC] # 90 298 962 # # HhaI: # [GCGC] # 2 13 48 280 1007 # # Hin4I: # [GAYNNNNNVTC] # 0 0 32 474 844 # # Hin4II*: # [CCTTC] # 81 1269 # # Hin6I: # [GCGC] # 2 11 48 282 1007 # # HindII: # [GTYRAC] # 132 300 417 501 # # HinfI: # [GANTC] # 5 20 103 206 209 238 # 283 286 # # HpaII: # [CCGG] # 78 167 190 392 523 # # HphI: # [GGTGA] # 347 406 597 # # Hpy166II: # [GTNNAC] # 19 132 139 155 278 300 # 327 # # Hpy178III*: # [TCNNGA] # 16 40 55 134 148 182 # 225 550 # # Hpy188I: # [TCNGA] # 21 42 66 79 119 155 # 180 318 370 # # Hpy99I: # [CGWCG] # 78 383 889 # # KpnI: # [GGTACC] # 94 1256 # # MaeII: # [ACGT] # 20 61 74 83 119 162 # 175 185 231 240 # # MaeIII: # [GTNAC] # 10 67 92 243 309 629 # # MboI: # [GATC] # 10 44 210 265 282 539 # # MboII: # [GAAGA] # 8 118 211 315 698 # # MlyI: # [GAGTC] # 5 26 280 447 592 # # MmeI: # [TCCRAC] # 317 508 525 # # MnlI: # [CCTC] # 57 97 158 161 209 297 # 371 # # MseI: # [TTAA] # 30 75 134 194 243 266 # 408 # # MslI: # [CAYNNNNRTG] # 497 853 # # MstI*: # [TGCGCA] # 12 1338 # # MwoI: # [GCNNNNNNNGC] # 9 174 1167 # # NlaIII: # [CATG] # 153 597 600 # # NlaIV: # [GGNNCC] # 15 40 92 127 283 793 # # PfoI: # [TCCNGGA] # 635 715 # # PleI: # [GAGTC] # 5 26 280 447 592 # # PmaCI: # [CACGTG] # 102 1248 # # RsaI: # [GTAC] # 92 106 110 158 383 501 # # ScrFI: # [CCNGG] # 111 128 526 585 # # SecI*: # [CCNNGG] # 168 242 940 # # SetI: # [ASST] # 7 10 15 20 20 31 # 39 43 60 61 77 80 # 80 101 125 162 204 215 # # SfaNI: # [GCATC] # 22 142 487 699 # # SgrAI: # [CRCCGGYG] # 191 1159 # # SspI: # [AATATT] # 306 1044 # # TaiI: # [ACGT] # 20 61 77 80 119 162 # 175 185 231 240 # # TaqI: # [TCGA] # 21 23 63 78 80 105 # 118 126 170 282 284 # # TaqII: # [CACCCA] # 72 376 432 470 # # TatI: # [WGTACW] # 160 473 717 # # TauI: # [GCSGC] # 56 1294 # # TfiI: # [GAWTC] # 123 206 495 526 # # TseI: # [GCWGC] # 3 3 32 34 67 136 # 145 167 763 # # Tsp45I: # [GTSAC] # 67 102 309 872 # # Tsp4CI*: # [ACNGT] # 9 120 208 285 296 432 # # TspDTI: # [ATGAA] # 118 612 620 # # TspEI: # [AATT] # 24 42 43 125 125 167 # 360 464 # # TspRI: # [CASTGNN] # 419 931 # # TstI: # [CACNNNNNNTCC] # 32 547 771 # # Tth111I: # [GACNNNGTC] # 93 413 844 # # VspI: # [ATTAAT] # 224 1126 # # ZraI: # [GACGTC] # 523 827 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 1350 # Reported_sequences: 1 # Reported_hitcount: 375 #---------------------------------------