Restriction Fragments for YELCtau1



Send questions or suggestions to SGD

BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YELCtau1 on chromosome V from coordinates 118551 to 118230.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.6681.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 16 25 + MslI CAYNNNNRTG 20 20 . . 19 24 + VspI ATTAAT 20 22 . . 20 23 + MseI TTAA 20 22 . . 23 27 + TspDTI ATGAA 38 36 . . 29 32 + SetI ASST 32 28 . . 30 33 + MnlI CCTC 40 39 . . 34 39 + Hpy166II GTNNAC 36 36 . . 34 39 + BssNAI GTATAC 36 36 . . 34 39 + AccI GTMKAC 35 37 . . 38 41 + TaiI ACGT 41 37 . . 38 41 + SetI ASST 41 37 . . 38 41 + MaeII ACGT 38 40 . . 42 45 + MseI TTAA 42 44 . . 53 58 + TatI WGTACW 53 57 . . 54 57 + Csp6I GTAC 54 56 . . 54 57 + RsaI GTAC 55 55 . . 65 69 - Hin4II* CCTTC 59 58 . . 71 76 + SspI AATATT 73 73 . . 76 79 + CviRI* TGCA 77 77 . . 81 85 + MaeIII GTNAC 80 85 . . 98 101 + CviJI RGCY 99 99 . . 99 104 + NmeAIII GCCGAG 125 123 . . 105 108 + MseI TTAA 105 107 . . 109 114 + XhoII RGATCY 109 113 . . 109 114 + BglII AGATCT 109 113 . . 110 113 + DpnI GATC 111 111 . . 110 113 + BstKTI GATC 112 110 . . 110 113 + MboI GATC 109 113 . . 117 122 + MfeI CAATTG 117 121 . . 118 121 + TspEI AATT 117 121 . . 130 133 + FatI CATG 129 133 . . 130 133 + NlaIII CATG 133 129 . . 130 133 + CviAII CATG 130 132 . . 137 141 + MaeIII GTNAC 136 141 . . 146 151 + SpeI ACTAGT 146 150 . . 147 150 + MaeI CTAG 147 149 . . 170 173 + MseI TTAA 170 172 . . 188 192 + Hpy188I TCNGA 190 189 . . 192 195 + TspEI AATT 191 195 . . 199 202 + TspEI AATT 198 202 . . 249 253 + BsmAI GTCTC 254 258 . . 258 262 + DdeI CTNAG 258 261 . . 280 283 + MseI TTAA 280 282 . . 282 287 + SspI AATATT 284 284 . . 298 301 + SetI ASST 301 297 . . 303 306 + MseI TTAA 303 305 . . 306 309 + SetI ASST 309 305 . . #--------------------------------------- # # # AccI: # [GTMKAC] # 35 287 # # BglII: # [AGATCT] # 109 213 # # BsmAI: # [GTCTC] # 68 254 # # BssNAI: # [GTATAC] # 36 286 # # BstKTI: # [GATC] # 112 210 # # Csp6I: # [GTAC] # 54 268 # # CviAII: # [CATG] # 130 192 # # CviJI: # [RGCY] # 99 223 # # CviRI*: # [TGCA] # 77 245 # # DdeI: # [CTNAG] # 64 258 # # DpnI: # [GATC] # 111 211 # # FatI: # [CATG] # 129 193 # # Hin4II*: # [CCTTC] # 59 263 # # Hpy166II: # [GTNNAC] # 36 286 # # Hpy188I: # [TCNGA] # 132 190 # # MaeI: # [CTAG] # 147 175 # # MaeII: # [ACGT] # 38 284 # # MaeIII: # [GTNAC] # 56 80 186 # # MboI: # [GATC] # 109 213 # # MfeI: # [CAATTG] # 117 205 # # MnlI: # [CCTC] # 40 282 # # MseI: # [TTAA] # 19 20 22 23 63 65 # 110 # # MslI: # [CAYNNNNRTG] # 20 302 # # NlaIII: # [CATG] # 133 189 # # NmeAIII: # [GCCGAG] # 125 197 # # RsaI: # [GTAC] # 55 267 # # SetI: # [ASST] # 8 9 13 32 260 # # SpeI: # [ACTAGT] # 146 176 # # SspI: # [AATATT] # 38 73 211 # # TaiI: # [ACGT] # 41 281 # # TatI: # [WGTACW] # 53 269 # # TspDTI: # [ATGAA] # 38 284 # # TspEI: # [AATT] # 7 74 117 124 # # VspI: # [ATTAAT] # 20 302 # # XhoII: # [RGATCY] # 109 213 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 322 # Reported_sequences: 1 # Reported_hitcount: 47 #---------------------------------------