Restriction Fragments for YELCdelta4



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The currently selected gene/sequence is :

YELCdelta4 on chromosome V from coordinates 135939 to 135612.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.30420.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 42 45 + TaiI ACGT 45 41 . . 42 45 + SetI ASST 45 41 . . 42 45 + MaeII ACGT 42 44 . . 44 48 + MaeIII GTNAC 43 48 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 74 78 + MboII GAAGA 86 85 . . 80 84 + HgaI GACGC 89 94 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 108 111 + TspEI AATT 107 111 . . 118 121 + SetI ASST 121 117 . . 118 121 + CviJI RGCY 119 119 . . 118 121 + AluI AGCT 119 119 . . 148 152 + BinI* GGATC 156 157 . . 149 152 + DpnI GATC 150 150 . . 149 152 + BstKTI GATC 151 149 . . 149 152 + MboI GATC 148 152 . . 154 158 + TspDTI ATGAA 169 167 . . 157 162 + SspI AATATT 159 159 . . 161 164 + MseI TTAA 161 163 . . 184 189 + SspI AATATT 186 186 . . 195 198 + TspEI AATT 194 198 . . 205 208 + TspEI AATT 204 208 . . 208 211 + CviRI* TGCA 209 209 . . 212 216 + TfiI GAWTC 212 215 . . 212 216 + HinfI GANTC 212 215 . . 216 226 + BsiYI* CCNNNNNNNGG 222 219 . . 226 230 + TfiI GAWTC 226 229 . . 226 230 + HinfI GANTC 226 229 . . 237 244 + PspXI VCTCGAGB 238 242 . . 237 240 + MnlI CCTC 247 246 . . 237 244 + AbsI CCTCGAGG 238 242 . . 238 243 + SmlI CTYRAG 238 242 . . 238 243 + XhoI CTCGAG 238 242 . . 238 243 + BmeT110I CYCGRG 239 241 . . 238 243 + AvaI CYCGRG 238 242 . . 239 242 + TaqI TCGA 239 241 . . 241 246 + BseRI GAGGAG 256 254 . . 241 244 - MnlI CCTC 234 233 . . 252 255 + MaeI CTAG 252 254 . . 267 270 + SetI ASST 270 266 . . 271 276 + SspI AATATT 273 273 . . 279 282 + CviJI RGCY 280 280 . . 283 286 + MseI TTAA 283 285 . . 295 299 + TfiI GAWTC 295 298 . . 295 299 + HinfI GANTC 295 298 . . 305 308 + TspEI AATT 304 308 . . #--------------------------------------- # # # AbsI: # [CCTCGAGG] # 90 238 # # AluI: # [AGCT] # 119 209 # # AvaI: # [CYCGRG] # 90 238 # # BinI*: # [GGATC] # 156 172 # # BmeT110I: # [CYCGRG] # 89 239 # # BseRI: # [GAGGAG] # 72 256 # # BsiYI*: # [CCNNNNNNNGG] # 106 222 # # BstKTI: # [GATC] # 151 177 # # CviJI: # [RGCY] # 48 119 161 # # CviRI*: # [TGCA] # 119 209 # # DpnI: # [GATC] # 150 178 # # HgaI: # [GACGC] # 89 239 # # Hin4I: # [GAYNNNNNVTC] # 32 83 213 # # HinfI: # [GANTC] # 14 33 69 212 # # MaeI: # [CTAG] # 15 33 76 204 # # MaeII: # [ACGT] # 42 286 # # MaeIII: # [GTNAC] # 43 285 # # MboI: # [GATC] # 148 180 # # MboII: # [GAAGA] # 86 242 # # MnlI: # [CCTC] # 13 81 234 # # MseI: # [TTAA] # 45 122 161 # # PspXI: # [VCTCGAGB] # 90 238 # # SetI: # [ASST] # 45 58 76 149 # # SmlI: # [CTYRAG] # 90 238 # # SpeI: # [ACTAGT] # 15 32 281 # # SspI: # [AATATT] # 27 55 87 159 # # TaiI: # [ACGT] # 45 283 # # TaqI: # [TCGA] # 89 239 # # TfiI: # [GAWTC] # 14 33 69 212 # # Tsp4CI*: # [ACNGT] # 67 261 # # TspDTI: # [ATGAA] # 159 169 # # TspEI: # [AATT] # 10 24 87 100 107 # # XhoI: # [CTCGAG] # 90 238 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 328 # Reported_sequences: 1 # Reported_hitcount: 50 #---------------------------------------