Restriction Fragments for EAF5/YEL018W



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

EAF5/YEL018W on chromosome V from coordinates 121471 to 122310.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.26250.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 11 14 + SetI ASST 14 10 . . 10 13 - MnlI CCTC 3 2 . . 20 23 + TspEI AATT 19 23 . . 32 35 + CviRI* TGCA 33 33 . . 33 37 + BlsI GCNGC 35 34 . . 33 37 + BisI GCNGC 34 35 . . 33 37 + BbvI GCAGC 45 49 . . 33 37 + TseI GCWGC 33 36 . . 35 38 + SetI ASST 38 34 . . 35 38 + CviJI RGCY 36 36 . . 35 38 + AluI AGCT 36 36 . . 49 52 + MseI TTAA 49 51 . . 51 54 + TspEI AATT 50 54 . . 55 60 + MmeI TCCRAC 80 78 . . 65 69 + TspDTI ATGAA 80 78 . . 67 71 + MboII GAAGA 79 78 . . 72 76 + BsrI ACTGG 77 75 . . 74 79 - TsoI TARCCA 64 62 . . 92 96 + TfiI GAWTC 92 95 . . 89 92 - MnlI CCTC 82 81 . . 92 96 + HinfI GANTC 92 95 . . 99 102 + FatI CATG 98 102 . . 99 102 + NlaIII CATG 102 98 . . 99 102 + CviAII CATG 99 101 . . 116 121 + AsuII TTCGAA 117 119 . . 117 120 + TaqI TCGA 117 119 . . 114 118 - MboII GAAGA 106 105 . . 127 130 + MaeI CTAG 127 129 . . 135 140 + Hpy166II GTNNAC 137 137 . . 135 140 + HindII GTYRAC 137 137 . . 157 160 + TspEI AATT 156 160 . . 173 176 + SetI ASST 176 172 . . 173 176 + CviJI RGCY 174 174 . . 173 176 + AluI AGCT 174 174 . . 180 185 + ApoI RAATTY 180 184 . . 181 184 + TspEI AATT 180 184 . . 184 187 + MseI TTAA 184 186 . . 188 192 - Hin4II* CCTTC 182 181 . . 200 203 + TaiI ACGT 203 199 . . 200 203 + SetI ASST 203 199 . . 200 203 + MaeII ACGT 200 202 . . 222 226 + BsrI ACTGG 227 225 . . 222 227 + BfiI ACTGGG 232 231 . . 227 231 + SfaNI GCATC 236 240 . . 235 240 + EcoRV GATATC 237 237 . . 239 244 + Hpy178III* TCNNGA 240 242 . . 251 254 + MseI TTAA 251 253 . . 255 264 + XmnI GAANNNNTTC 259 259 . . 255 260 + ApoI RAATTY 255 259 . . 256 259 + TspEI AATT 255 259 . . 269 274 + TatI WGTACW 269 273 . . 270 273 + Csp6I GTAC 270 272 . . 270 273 + RsaI GTAC 271 271 . . 289 293 - BccI CCATC 283 284 . . 293 304 + DrdI GACNNNNNNGTC 299 297 . . 298 302 + DdeI CTNAG 298 301 . . 302 307 + Hpy166II GTNNAC 304 304 . . 302 307 + AccI GTMKAC 303 305 . . 308 312 + Tsp4CI* ACNGT 310 309 . . 319 324 + EcoT22I ATGCAT 323 319 . . 320 323 + CviRI* TGCA 321 321 . . 322 325 + FatI CATG 321 325 . . 322 325 + NlaIII CATG 325 321 . . 322 325 + CviAII CATG 322 324 . . 325 330 + EcoRV GATATC 327 327 . . 329 332 + TaqI TCGA 329 331 . . 329 334 + Hpy178III* TCNNGA 330 332 . . 335 338 + SetI ASST 338 334 . . 335 338 + CviJI RGCY 336 336 . . 335 338 + AluI AGCT 336 336 . . 351 354 + Csp6I GTAC 351 353 . . 351 354 + RsaI GTAC 352 352 . . 354 359 + McrI* CGRYCG 357 355 . . 354 359 + PvuI CGATCG 357 355 . . 355 358 + DpnI GATC 356 356 . . 355 358 + BstKTI GATC 357 355 . . 355 358 + MboI GATC 354 358 . . 356 361 + ClaI ATCGAT 357 359 . . 357 360 + TaqI TCGA 357 359 . . 359 363 + TfiI GAWTC 359 362 . . 359 363 + HinfI GANTC 359 362 . . 362 367 + Hpy178III* TCNNGA 363 365 . . 374 377 + SetI ASST 377 373 . . 377 380 + TaqI TCGA 377 379 . . 372 377 - BspMI ACCTGC 363 367 . . 389 393 + FokI GGATG 402 406 . . 389 393 + BseGI GGATG 395 393 . . 393 397 - BccI CCATC 387 388 . . 399 403 + FokI GGATG 412 416 . . 399 403 + BseGI GGATG 405 403 . . 397 400 - MnlI CCTC 390 389 . . 411 415 + MboII GAAGA 423 422 . . 416 420 + Tsp4CI* ACNGT 418 417 . . 427 432 + Cfr10I RCCGGY 427 431 . . 427 432 + BetI* WCCGGW 427 431 . . 427 432 + AgeI ACCGGT 427 431 . . 428 431 + HpaII CCGG 428 430 . . 432 435 + CviRI* TGCA 433 433 . . 433 437 + SfaNI GCATC 442 446 . . 434 439 - BtgZI GCGATG 419 423 . . 443 446 + CviRI* TGCA 444 444 . . 444 455 + AlfI GCANNNNNNTGC 433 431 467 465 450 455 + NspI RCATGY 454 450 . . 451 460 + MslI CAYNNNNRTG 455 455 . . 451 454 + FatI CATG 450 454 . . 451 454 + NlaIII CATG 454 450 . . 451 454 + CviAII CATG 451 453 . . 453 456 + CviRI* TGCA 454 454 . . 444 455 - AlfI GCANNNNNNTGC 467 465 433 431 460 465 + Cac8I GCNNGC 462 462 . . 472 477 + XhoII RGATCY 472 476 . . 472 476 + BinI* GGATC 480 481 . . 473 476 + DpnI GATC 474 474 . . 473 476 + BstKTI GATC 475 473 . . 473 476 + MboI GATC 472 476 . . 481 486 + KpnI GGTACC 485 481 . . 476 481 - EcoP15I CAGCAG 448 450 . . 481 486 + NlaIV GGNNCC 483 483 . . 481 486 + HgiCI* GGYRCC 481 485 . . 481 486 + Acc65I GGTACC 481 485 . . 482 485 + Csp6I GTAC 482 484 . . 482 485 + RsaI GTAC 483 483 . . 486 492 + TspRI CASTGNN 492 483 . . 485 489 - BsrI ACTGG 485 483 . . 516 519 + FatI CATG 515 519 . . 516 519 + NlaIII CATG 519 515 . . 516 519 + CviAII CATG 516 518 . . 520 523 + Hin6I GCGC 520 522 . . 520 523 + GlaI GCGC 521 521 . . 520 523 + HhaI GCGC 522 520 . . 522 527 - BsmI GAATGC 522 520 . . 531 536 + NlaIV GGNNCC 533 533 . . 531 535 + BmgT120I GGNCC 532 533 . . 531 535 + AvaII GGWCC 531 534 . . 531 535 + AsuI* GGNCC 531 534 . . 557 560 + TspEI AATT 556 560 . . 571 575 + Tsp4CI* ACNGT 573 572 . . 576 580 + Hpy188I TCNGA 578 577 . . 566 577 - PpiI GAACNNNNNCTC 589 584 557 552 584 587 + TspEI AATT 583 587 . . 598 602 + BsrI ACTGG 603 601 . . 598 603 + BfiI ACTGGG 608 607 . . 603 608 + ApoI RAATTY 603 607 . . 604 607 + TspEI AATT 603 607 . . 609 613 + Hpy188I TCNGA 611 610 . . 612 621 + XmnI GAANNNNTTC 616 616 . . 614 617 + SetI ASST 617 613 . . 614 617 + CviJI RGCY 615 615 . . 614 617 + AluI AGCT 615 615 . . 620 625 + Hpy178III* TCNNGA 621 623 . . 624 628 + PleI GAGTC 632 633 . . 624 628 + MlyI GAGTC 633 633 . . 624 628 + HinfI GANTC 624 627 . . 627 632 + Hpy178III* TCNNGA 628 630 . . 626 630 - BslFI GGGAC 611 615 . . 631 635 - BccI CCATC 625 626 . . 644 649 + Hpy178III* TCNNGA 645 647 . . 658 661 + TspEI AATT 657 661 . . 664 668 + MboII GAAGA 676 675 . . 664 669 + BbvII* GAAGAC 671 675 . . 685 689 + BssKI CCNGG 684 689 . . 685 689 + ScrFI CCNGG 686 687 . . 685 689 + CauII* CCSGG 686 687 . . 686 689 + HpaII CCGG 686 688 . . 689 693 + BlsI GCNGC 691 690 . . 689 693 + BisI GCNGC 690 691 . . 689 693 + BbvI GCAGC 701 705 . . 689 693 + TseI GCWGC 689 692 . . 696 701 + Hpy178III* TCNNGA 697 699 . . 696 701 + BspHI TCATGA 696 700 . . 697 700 + FatI CATG 696 700 . . 697 700 + NlaIII CATG 700 696 . . 697 700 + CviAII CATG 697 699 . . 698 702 + TspDTI ATGAA 713 711 . . 695 699 - TspDTI ATGAA 685 683 . . 703 708 + PstI CTGCAG 707 703 . . 703 708 + SfeI* CTRYAG 703 707 . . 704 707 + CviRI* TGCA 705 705 . . 705 709 + BlsI GCNGC 707 706 . . 705 709 + BisI GCNGC 706 707 . . 705 709 + BbvI GCAGC 717 721 . . 705 709 + TseI GCWGC 705 708 . . 706 711 + PvuII CAGCTG 708 708 . . 706 711 + NspBII* CMGCKG 708 708 . . 707 710 + SetI ASST 710 706 . . 707 710 + CviJI RGCY 708 708 . . 707 710 + AluI AGCT 708 708 . . 711 715 + TfiI GAWTC 711 714 . . 711 715 + HinfI GANTC 711 714 . . 714 718 - MboII GAAGA 706 705 . . 730 734 + TspGWI ACGGA 745 743 . . 734 738 + Tsp4CI* ACNGT 736 735 . . 749 752 + SetI ASST 752 748 . . 758 761 + Csp6I GTAC 758 760 . . 758 761 + RsaI GTAC 759 759 . . 760 764 + TspGWI ACGGA 775 773 . . 767 771 + BsrI ACTGG 772 770 . . 773 776 + TspEI AATT 772 776 . . 777 780 + CviJI RGCY 778 778 . . 779 782 + MaeI CTAG 779 781 . . 776 781 - TsoI TARCCA 766 764 . . 787 792 + BsgI GTGCAG 808 806 . . 788 791 + CviRI* TGCA 789 789 . . 797 800 + TspEI AATT 796 800 . . 801 805 + FokI GGATG 814 818 . . 801 805 + BseGI GGATG 807 805 . . 808 812 + Tsp4CI* ACNGT 810 809 . . 811 816 + BsgI GTGCAG 832 830 . . 812 815 + CviRI* TGCA 813 813 . . 818 823 + Hpy178III* TCNNGA 819 821 . . 818 823 + BspHI TCATGA 818 822 . . 819 822 + FatI CATG 818 822 . . 819 822 + NlaIII CATG 822 818 . . 819 822 + CviAII CATG 819 821 . . 817 821 - TspDTI ATGAA 807 805 . . 824 828 + MboII GAAGA 836 835 . . 827 832 + SduI GDGCHC 831 827 . . 827 832 + HgiAI* GWGCWC 831 827 . . 824 829 - Ksp632I* CTCTTC 819 822 . . 824 830 - SapI GCTCTTC 819 822 . . 832 835 + MaeI CTAG 832 834 . . #--------------------------------------- # # # Acc65I: # [GGTACC] # 359 481 # # AccI: # [GTMKAC] # 303 537 # # AgeI: # [ACCGGT] # 413 427 # # AlfI: # [GCANNNNNNTGC] # 0 0 34 373 433 # # AluI: # [AGCT] # 36 93 132 138 162 279 # # ApoI: # [RAATTY] # 75 180 237 348 # # AsuI*: # [GGNCC] # 309 531 # # AsuII: # [TTCGAA] # 117 723 # # AvaII: # [GGWCC] # 309 531 # # BbvI: # [GCAGC] # 16 45 123 656 # # BbvII*: # [GAAGAC] # 169 671 # # BccI: # [CCATC] # 104 215 238 283 # # BetI*: # [WCCGGW] # 413 427 # # BfiI: # [ACTGGG] # 232 232 376 # # BinI*: # [GGATC] # 360 480 # # BisI: # [GCNGC] # 16 34 134 656 # # BlsI: # [GCNGC] # 16 35 133 656 # # BmgT120I: # [GGNCC] # 308 532 # # BseGI: # [GGATG] # 10 33 395 402 # # BsgI: # [GTGCAG] # 8 24 808 # # BslFI: # [GGGAC] # 229 611 # # BsmI: # [GAATGC] # 318 522 # # BspHI: # [TCATGA] # 22 122 696 # # BspMI: # [ACCTGC] # 363 477 # # BsrI: # [ACTGG] # 68 77 118 150 169 258 # # BssKI: # [CCNGG] # 156 684 # # BstKTI: # [GATC] # 118 357 365 # # BtgZI: # [GCGATG] # 419 421 # # Cac8I: # [GCNNGC] # 378 462 # # CauII*: # [CCSGG] # 154 686 # # Cfr10I: # [RCCGGY] # 413 427 # # ClaI: # [ATCGAT] # 357 483 # # Csp6I: # [GTAC] # 81 82 131 270 276 # # CviAII: # [CATG] # 21 65 99 122 129 181 # 223 # # CviJI: # [RGCY] # 36 62 70 93 138 162 # 279 # # CviRI*: # [TGCA] # 10 11 24 27 33 84 # 112 251 288 # # DdeI: # [CTNAG] # 298 542 # # DpnI: # [GATC] # 118 356 366 # # DrdI: # [GACNNNNNNGTC] # 299 541 # # EcoP15I: # [CAGCAG] # 392 448 # # EcoRV: # [GATATC] # 90 237 513 # # EcoT22I: # [ATGCAT] # 323 517 # # FatI: # [CATG] # 22 65 98 122 129 181 # 223 # # FokI: # [GGATG] # 10 26 402 402 # # GlaI: # [GCGC] # 319 521 # # HgiAI*: # [GWGCWC] # 9 831 # # HgiCI*: # [GGYRCC] # 359 481 # # HhaI: # [GCGC] # 318 522 # # Hin4II*: # [CCTTC] # 182 658 # # Hin6I: # [GCGC] # 320 520 # # HindII: # [GTYRAC] # 137 703 # # HinfI: # [GANTC] # 87 92 129 265 267 # # HpaII: # [CCGG] # 154 258 428 # # Hpy166II: # [GTNNAC] # 137 167 536 # # Hpy178III*: # [TCNNGA] # 7 17 21 33 52 90 # 122 240 258 # # Hpy188I: # [TCNGA] # 33 229 578 # # KpnI: # [GGTACC] # 355 485 # # Ksp632I*: # [CTCTTC] # 21 819 # # MaeI: # [CTAG] # 8 53 127 652 # # MaeII: # [ACGT] # 200 640 # # MboI: # [GATC] # 118 354 368 # # MboII: # [GAAGA] # 4 27 30 79 130 253 # 317 # # McrI*: # [CGRYCG] # 357 483 # # MlyI: # [GAGTC] # 207 633 # # MmeI: # [TCCRAC] # 80 760 # # MnlI: # [CCTC] # 3 79 308 450 # # MseI: # [TTAA] # 49 67 135 589 # # MslI: # [CAYNNNNRTG] # 385 455 # # NlaIII: # [CATG] # 18 65 102 122 129 181 # 223 # # NlaIV: # [GGNNCC] # 50 307 483 # # NspBII*: # [CMGCKG] # 132 708 # # NspI: # [RCATGY] # 386 454 # # PleI: # [GAGTC] # 208 632 # # PpiI: # [GAACNNNNNCTC] # 32 251 557 # # PstI: # [CTGCAG] # 133 707 # # PvuI: # [CGATCG] # 357 483 # # PvuII: # [CAGCTG] # 132 708 # # RsaI: # [GTAC] # 81 81 131 271 276 # # SapI: # [GCTCTTC] # 21 819 # # ScrFI: # [CCNGG] # 154 686 # # SduI: # [GDGCHC] # 9 831 # # SetI: # [ASST] # 14 24 27 39 42 88 # 93 135 138 240 # # SfaNI: # [GCATC] # 206 236 398 # # SfeI*: # [CTRYAG] # 137 703 # # TaiI: # [ACGT] # 203 637 # # TaqI: # [TCGA] # 20 28 117 212 463 # # TatI: # [WGTACW] # 269 571 # # TfiI: # [GAWTC] # 92 129 267 352 # # TseI: # [GCWGC] # 16 33 135 656 # # TsoI: # [TARCCA] # 64 74 702 # # Tsp4CI*: # [ACNGT] # 30 74 108 155 163 310 # # TspDTI: # [ATGAA] # 28 33 80 94 605 # # TspEI: # [AATT] # 19 20 24 24 27 31 # 44 54 75 106 115 301 # # TspGWI: # [ACGGA] # 30 65 745 # # TspRI: # [CASTGNN] # 348 492 # # XhoII: # [RGATCY] # 368 472 # # XmnI: # [GAANNNNTTC] # 224 259 357 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 840 # Reported_sequences: 1 # Reported_hitcount: 221 #---------------------------------------