Restriction Fragments for YDRWdelta7



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BLAST search | Genome Restriction Map | Design Primers for this sequence


The currently selected gene/sequence is :

YDRWdelta7 on chromosome IV from coordinates 520477 to 520805.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.14936.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 32 37 + SpeI ACTAGT 32 36 . . 33 36 + MaeI CTAG 33 35 . . 44 48 + MaeIII GTNAC 43 48 . . 47 52 + SpeI ACTAGT 47 51 . . 48 51 + MaeI CTAG 48 50 . . 65 69 + Tsp4CI* ACNGT 67 66 . . 74 78 + MboII GAAGA 86 85 . . 92 102 - Hin4I GAYNNNNNVTC 115 110 83 78 108 111 + TspEI AATT 107 111 . . 153 157 + TspDTI ATGAA 168 166 . . 174 177 - MnlI CCTC 167 166 . . 181 184 + DpnI GATC 182 182 . . 181 184 + BstKTI GATC 183 181 . . 181 184 + MboI GATC 180 184 . . 174 184 - Hin4I GAYNNNNNVTC 197 192 165 160 187 192 + SspI AATATT 189 189 . . 194 199 + NspI RCATGY 198 194 . . 195 198 + FatI CATG 194 198 . . 195 198 + NlaIII CATG 198 194 . . 195 198 + CviAII CATG 195 197 . . 209 213 + TfiI GAWTC 209 212 . . 209 213 + HinfI GANTC 209 212 . . 213 223 + BsiYI* CCNNNNNNNGG 219 216 . . 223 227 + TfiI GAWTC 223 226 . . 223 227 + HinfI GANTC 223 226 . . 233 239 + PfoI TCCNGGA 233 238 . . 234 238 + BssKI CCNGG 233 238 . . 234 238 + BseBI CCWGG 235 236 . . 234 238 + ScrFI CCNGG 235 236 . . 234 238 + EcoRII CCWGG 233 238 . . 235 240 + Eco57MI CTGRAG 256 254 . . 235 240 + GsuI CTGGAG 256 254 . . 238 243 + BseRI GAGGAG 253 251 . . 238 241 - MnlI CCTC 231 230 . . 249 252 + MaeI CTAG 249 251 . . 264 267 + SetI ASST 267 263 . . 268 273 + SspI AATATT 270 270 . . 283 286 + MseI TTAA 283 285 . . 292 296 + TfiI GAWTC 292 295 . . 292 296 + HinfI GANTC 292 295 . . 302 305 + TspEI AATT 301 305 . . 309 313 + Hpy188I TCNGA 311 310 . . 312 316 + TfiI GAWTC 312 315 . . 312 316 + HinfI GANTC 312 315 . . 316 319 + AciI CCGC 316 318 . . 322 325 + TspEI AATT 321 325 . . #--------------------------------------- # # # AciI: # [CCGC] # 13 316 # # BseBI: # [CCWGG] # 94 235 # # BseRI: # [GAGGAG] # 76 253 # # BsiYI*: # [CCNNNNNNNGG] # 110 219 # # BssKI: # [CCNGG] # 96 233 # # BstKTI: # [GATC] # 146 183 # # CviAII: # [CATG] # 134 195 # # DpnI: # [GATC] # 147 182 # # Eco57MI: # [CTGRAG] # 73 256 # # EcoRII: # [CCWGG] # 96 233 # # FatI: # [CATG] # 135 194 # # GsuI: # [CTGGAG] # 73 256 # # Hin4I: # [GAYNNNNNVTC] # 32 32 50 83 132 # # HinfI: # [GANTC] # 14 17 20 69 209 # # Hpy188I: # [TCNGA] # 18 311 # # MaeI: # [CTAG] # 15 33 80 201 # # MaeIII: # [GTNAC] # 43 286 # # MboI: # [GATC] # 149 180 # # MboII: # [GAAGA] # 86 243 # # MnlI: # [CCTC] # 64 98 167 # # MseI: # [TTAA] # 46 283 # # NlaIII: # [CATG] # 131 198 # # NspI: # [RCATGY] # 131 198 # # PfoI: # [TCCNGGA] # 96 233 # # ScrFI: # [CCNGG] # 94 235 # # SetI: # [ASST] # 62 267 # # SpeI: # [ACTAGT] # 15 32 282 # # SspI: # [AATATT] # 59 81 189 # # TfiI: # [GAWTC] # 14 17 20 69 209 # # Tsp4CI*: # [ACNGT] # 67 262 # # TspDTI: # [ATGAA] # 161 168 # # TspEI: # [AATT] # 8 20 107 194 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 329 # Reported_sequences: 1 # Reported_hitcount: 46 #---------------------------------------