Restriction Fragments for YDRCsigma6



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The currently selected gene/sequence is :

YDRCsigma6 on chromosome IV from coordinates 1175812 to 1175134.

The sequence you have requested is oriented with respect to the Crick (bottom) strand. Thus, the chromosomal coordinate of the beginning of the gene or sequence is larger than that of the end. The sequence shown here is the 5'-3' direction of the Crick strand and is the reverse complement of the Watson (top) strand.

seqfile=/share/crumb/www-data/html/tmp/gcgseq.tmp.2.26983.2

Start End Strand Enzyme_name Restriction_site 5prime 3prime 5primerev 3primerev 8 12 + DdeI CTNAG 8 11 . . 8 12 + BspCNI CTCAG 21 19 . . 8 12 + BseMII CTCAG 22 20 . . 9 13 + Hpy188I TCNGA 11 10 . . 21 24 + TaiI ACGT 24 20 . . 21 24 + SetI ASST 24 20 . . 21 24 + MaeII ACGT 21 23 . . 17 22 - BciVI GTATCC 11 10 . . 28 31 + FatI CATG 27 31 . . 28 31 + NlaIII CATG 31 27 . . 28 31 + CviAII CATG 28 30 . . 43 48 + Hpy178III* TCNNGA 44 46 . . 48 53 + AclI AACGTT 49 51 . . 49 52 + TaiI ACGT 52 48 . . 49 52 + SetI ASST 52 48 . . 49 52 + MaeII ACGT 49 51 . . 52 56 - TspDTI ATGAA 42 40 . . 63 68 + NdeI CATATG 64 66 . . 66 70 + TspDTI ATGAA 81 79 . . 71 74 + SetI ASST 74 70 . . 73 77 + DdeI CTNAG 73 76 . . 98 101 + FatI CATG 97 101 . . 98 101 + NlaIII CATG 101 97 . . 98 101 + CviAII CATG 98 100 . . 102 105 + SetI ASST 105 101 . . 101 104 - MnlI CCTC 94 93 . . 112 115 + AciI CCGC 112 114 . . 115 119 + Hin4II* CCTTC 125 124 . . 111 116 - EciI GGCGGA 101 99 . . 119 124 + SfeI* CTRYAG 119 123 . . 123 126 + SetI ASST 126 122 . . 123 126 + CviJI RGCY 124 124 . . 123 126 + AluI AGCT 124 124 . . 128 131 + CviJI RGCY 129 129 . . 124 129 - GsaI CCCAGC 128 124 . . 124 129 - BseYI CCCAGC 124 128 . . 138 141 + TaiI ACGT 141 137 . . 138 141 + SetI ASST 141 137 . . 138 141 + MaeII ACGT 138 140 . . 155 160 + MfeI CAATTG 155 159 . . 156 159 + TspEI AATT 155 159 . . 173 178 - TaqII CACCCA 163 161 . . 187 191 - BsmAI GTCTC 181 185 . . 199 203 + TfiI GAWTC 199 202 . . 199 203 + HinfI GANTC 199 202 . . 217 220 + CviJI RGCY 218 218 . . 223 228 + SspI AATATT 225 225 . . 229 232 + SetI ASST 232 228 . . 231 236 + Hpy166II GTNNAC 233 233 . . 232 237 + TatI WGTACW 232 236 . . 232 237 + Bsp1407I TGTACA 232 236 . . 233 236 + Csp6I GTAC 233 235 . . 233 236 + RsaI GTAC 234 234 . . 245 248 + MaeI CTAG 245 247 . . 256 259 + MnlI CCTC 266 265 . . 258 261 + TaqI TCGA 258 260 . . 254 259 - BseRI GAGGAG 245 243 . . 270 274 + TfiI GAWTC 270 273 . . 270 274 + HinfI GANTC 270 273 . . 274 277 + MnlI CCTC 284 283 . . 274 284 + BsiYI* CCNNNNNNNGG 280 277 . . 284 288 + TfiI GAWTC 284 287 . . 284 288 + HinfI GANTC 284 287 . . 305 310 + SspI AATATT 307 307 . . 313 323 + Hin4I GAYNNNNNVTC 304 299 336 331 320 323 + MnlI CCTC 330 329 . . 326 330 - TspGWI ACGGA 316 314 . . 340 344 - TspDTI ATGAA 330 328 . . 368 371 + CviRI* TGCA 369 369 . . 376 379 + SetI ASST 379 375 . . 376 379 + CviJI RGCY 377 377 . . 376 379 + AluI AGCT 377 377 . . 372 377 - Eco57MI CTGRAG 357 355 . . 372 377 - Eco57I CTGAAG 357 355 . . 381 384 + MnlI CCTC 391 390 . . 390 393 + TaqI TCGA 390 392 . . 395 405 + Hin4I GAYNNNNNVTC 386 381 418 413 398 401 + SetI ASST 401 397 . . 398 401 + CviJI RGCY 399 399 . . 398 401 + AluI AGCT 399 399 . . 424 427 + MseI TTAA 424 426 . . 429 433 + Tsp4CI* ACNGT 431 430 . . 452 457 + TatI WGTACW 452 456 . . 453 456 + Csp6I GTAC 453 455 . . 453 456 + RsaI GTAC 454 454 . . 482 486 + TfiI GAWTC 482 485 . . 482 486 + HinfI GANTC 482 485 . . 491 496 + MmeI TCCRAC 516 514 . . 495 499 + Tsp4CI* ACNGT 497 496 . . 502 507 + XhoII RGATCY 502 506 . . 503 506 + DpnI GATC 504 504 . . 503 506 + BstKTI GATC 505 503 . . 503 506 + MboI GATC 502 506 . . 506 511 + StyI CCWWGG 506 510 . . 506 511 + SecI* CCNNGG 506 510 . . 503 507 - BinI* GGATC 497 498 . . 509 512 + SetI ASST 512 508 . . 510 515 + NlaIV GGNNCC 512 512 . . 514 517 + AciI CCGC 514 516 . . 515 518 + FnuDII* CGCG 516 516 . . 516 519 + Hin6I GCGC 516 518 . . 516 519 + GlaI GCGC 517 517 . . 516 519 + HhaI GCGC 518 516 . . 536 539 + DpnI GATC 537 537 . . 536 539 + BstKTI GATC 538 536 . . 536 539 + MboI GATC 535 539 . . 536 540 - BinI* GGATC 530 531 . . 566 570 + MaeIII GTNAC 565 570 . . 573 577 - TspGWI ACGGA 563 561 . . 579 582 + TspEI AATT 578 582 . . 580 585 + VspI ATTAAT 581 583 . . 581 584 + MseI TTAA 581 583 . . 585 589 + Hpy188I TCNGA 587 586 . . 593 597 + Tsp4CI* ACNGT 595 594 . . 605 609 + BsrI ACTGG 610 608 . . 609 613 + MaeIII GTNAC 608 613 . . 607 612 - TsoI TARCCA 597 595 . . 618 622 + DdeI CTNAG 618 621 . . 623 627 + Tsp4CI* ACNGT 625 624 . . 642 645 + TaqI TCGA 642 644 . . 642 647 + Hpy178III* TCNNGA 643 645 . . 644 648 - BsmAI GTCTC 638 642 . . 651 656 + BetI* WCCGGW 651 655 . . 652 655 + HpaII CCGG 652 654 . . 661 666 + SmlI CTYRAG 661 665 . . 664 669 + SduI GDGCHC 668 664 . . 664 669 + HgiJII* GRGCYC 668 664 . . 665 668 + CviJI RGCY 666 666 . . 672 677 + TatI WGTACW 672 676 . . 673 676 + Csp6I GTAC 673 675 . . 673 676 + RsaI GTAC 674 674 . . #--------------------------------------- # # # AciI: # [CCGC] # 112 165 402 # # AclI: # [AACGTT] # 49 630 # # AluI: # [AGCT] # 22 124 253 280 # # BciVI: # [GTATCC] # 11 668 # # BetI*: # [WCCGGW] # 28 651 # # BinI*: # [GGATC] # 33 149 497 # # BseMII: # [CTCAG] # 22 657 # # BseRI: # [GAGGAG] # 245 434 # # BseYI: # [CCCAGC] # 124 555 # # BsiYI*: # [CCNNNNNNNGG] # 280 399 # # BsmAI: # [GTCTC] # 41 181 457 # # Bsp1407I: # [TGTACA] # 232 447 # # BspCNI: # [CTCAG] # 21 658 # # BsrI: # [ACTGG] # 69 610 # # BstKTI: # [GATC] # 33 141 505 # # Csp6I: # [GTAC] # 6 220 220 233 # # CviAII: # [CATG] # 28 70 581 # # CviJI: # [RGCY] # 5 13 22 89 124 159 # 267 # # CviRI*: # [TGCA] # 310 369 # # DdeI: # [CTNAG] # 8 61 65 545 # # DpnI: # [GATC] # 33 142 504 # # EciI: # [GGCGGA] # 101 578 # # Eco57I: # [CTGAAG] # 322 357 # # Eco57MI: # [CTGRAG] # 322 357 # # FatI: # [CATG] # 27 70 582 # # FnuDII*: # [CGCG] # 163 516 # # GlaI: # [GCGC] # 162 517 # # GsaI: # [CCCAGC] # 128 551 # # HgiJII*: # [GRGCYC] # 11 668 # # HhaI: # [GCGC] # 161 518 # # Hin4I: # [GAYNNNNNVTC] # 32 32 50 261 304 # # Hin4II*: # [CCTTC] # 125 554 # # Hin6I: # [GCGC] # 163 516 # # HinfI: # [GANTC] # 14 71 197 198 199 # # HpaII: # [CCGG] # 27 652 # # Hpy166II: # [GTNNAC] # 233 446 # # Hpy178III*: # [TCNNGA] # 36 44 599 # # Hpy188I: # [TCNGA] # 11 92 576 # # MaeI: # [CTAG] # 245 434 # # MaeII: # [ACGT] # 21 28 89 541 # # MaeIII: # [GTNAC] # 43 71 565 # # MboI: # [GATC] # 33 144 502 # # MfeI: # [CAATTG] # 155 524 # # MmeI: # [TCCRAC] # 163 516 # # MnlI: # [CCTC] # 18 46 61 94 172 288 # # MseI: # [TTAA] # 98 157 424 # # NdeI: # [CATATG] # 64 615 # # NlaIII: # [CATG] # 31 70 578 # # NlaIV: # [GGNNCC] # 167 512 # # RsaI: # [GTAC] # 5 220 220 234 # # SduI: # [GDGCHC] # 11 668 # # SecI*: # [CCNNGG] # 173 506 # # SetI: # [ASST] # 15 21 22 22 24 28 # 31 91 111 147 167 # # SfeI*: # [CTRYAG] # 119 560 # # SmlI: # [CTYRAG] # 18 661 # # SspI: # [AATATT] # 82 225 372 # # StyI: # [CCWWGG] # 173 506 # # TaiI: # [ACGT] # 24 28 89 538 # # TaqI: # [TCGA] # 37 132 252 258 # # TaqII: # [CACCCA] # 163 516 # # TatI: # [WGTACW] # 7 220 220 232 # # TfiI: # [GAWTC] # 14 71 197 198 199 # # TsoI: # [TARCCA] # 82 597 # # Tsp4CI*: # [ACNGT] # 30 54 66 98 431 # # TspDTI: # [ATGAA] # 39 42 249 349 # # TspEI: # [AATT] # 101 155 423 # # TspGWI: # [ACGGA] # 116 247 316 # # VspI: # [ATTAAT] # 98 581 # # XhoII: # [RGATCY] # 177 502 # #--------------------------------------- #--------------------------------------- # Total_sequences: 1 # Total_length: 679 # Reported_sequences: 1 # Reported_hitcount: 131 #---------------------------------------